n protein  (Sino Biological)


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    Name:
    Influenza B Nucleoprotein NP Protein
    Description:
    A DNA sequence encoding the Influenza B B Florida 4 2006 nucleoprotein ACF54251 1 Met1 Tyr560 was expressed with a polyhistidine tag at the C terminus
    Catalog Number:
    40438-v08b
    Product Aliases:
    NP Protein Influenza B, Nucleoprotein Protein Influenza B
    Price:
    None
    Host:
    Baculovirus-Insect Cells
    Category:
    recombinant protein
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    Structured Review

    Sino Biological n protein
    A DNA sequence encoding the Influenza B B Florida 4 2006 nucleoprotein ACF54251 1 Met1 Tyr560 was expressed with a polyhistidine tag at the C terminus
    https://www.bioz.com/result/n protein/product/Sino Biological
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    n protein - by Bioz Stars, 2021-02
    93/100 stars

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    Related Articles

    Amplification:

    Article Title: An in vivo cell-based assay for investigating the specific interaction between the SARS-CoV N-protein and its viral RNA packaging sequence.
    Article Snippet: .. Packaging signal sequences for all reporter plasmids are listed in Supplementary Table 2. ii) Construction of protein expression plasmids The N-protein sequence was amplified via PCR from pCMV-SARS-N (Sino Biological Inc., Beijing, China), a vector encoding the full-length human SARS coronavirus, following codon-optimization to increase the level of protein expression in mammalian cells. .. The amplicon was then digested with Xba I and Hind III prior to insertion in a pTAC plasmid (Sigma Aldrich, MO, USA) to generate pTAC-SARS-N.

    Binding Assay:

    Article Title: Bioassay Development for Ultrasensitive Detection of Influenza A Nucleoprotein Using Digital ELISA.
    Article Snippet: .. Seven commercially available antibodies were selected for testing their binding affinity toward recombinant influenza A nucleoprotein: one monoclonal (11675-MM03, referred to as SinoM) and one polyclonal (11675-RP01, referred to as SinoP) antibody were purchased from SinoBiological Inc. (China); four monoclonal antibodies (ab110661, ab128193, ab66191, ab67428, in this paper referred to as Ab11, Ab12, Ab66, and Ab67, respectively) were purchased from Abcam plc. (United Kingdom); a polyclonal antibody (IMG-5134A, referred to as ImP) was purchased from Imgenex (United States). .. The selection of antibodies was based on a high variety of possible applications stated in the datasheet (ELISA, Western blot, flow cytometry, ...).

    Produced:

    Article Title: Kinetics of SARS-CoV-2 specific IgM and IgG responses in COVID-19 patients
    Article Snippet: .. N protein (residue 1–419) was produced from Baculovirus-Insect Cells (Cat. # 40588-V08B, Sino biological, Beijing, China). .. S protein (residue 16–685) was produced from HEK293 Cells (Cat. #40591-V08H, Sino biological, Beijing, China).

    Sequencing:

    Article Title: An in vivo cell-based assay for investigating the specific interaction between the SARS-CoV N-protein and its viral RNA packaging sequence.
    Article Snippet: .. Packaging signal sequences for all reporter plasmids are listed in Supplementary Table 2. ii) Construction of protein expression plasmids The N-protein sequence was amplified via PCR from pCMV-SARS-N (Sino Biological Inc., Beijing, China), a vector encoding the full-length human SARS coronavirus, following codon-optimization to increase the level of protein expression in mammalian cells. .. The amplicon was then digested with Xba I and Hind III prior to insertion in a pTAC plasmid (Sigma Aldrich, MO, USA) to generate pTAC-SARS-N.

    Generated:

    Article Title: Heterogeneous antibodies against SARS-CoV-2 spike receptor binding domain and nucleocapsid with implications on COVID-19 immunity.
    Article Snippet: .. ELISAMaxiSorp C-shaped 96-wells plates were coated with S-RBD, S-RBD1 or N-protein generated from plasmids from Sino Biologicals and Addgene. ..

    Article Title: Heterogeneous antibodies against SARS-CoV-2 spike receptor binding domain and nucleocapsid with implications for COVID-19 immunity
    Article Snippet: .. MaxiSorp C-shaped 96-well plates were coated with S-RBD, S-RBD1, or N-protein generated from plasmids from Sino Biological and Addgene. ..

    Expressing:

    Article Title: An in vivo cell-based assay for investigating the specific interaction between the SARS-CoV N-protein and its viral RNA packaging sequence.
    Article Snippet: .. Packaging signal sequences for all reporter plasmids are listed in Supplementary Table 2. ii) Construction of protein expression plasmids The N-protein sequence was amplified via PCR from pCMV-SARS-N (Sino Biological Inc., Beijing, China), a vector encoding the full-length human SARS coronavirus, following codon-optimization to increase the level of protein expression in mammalian cells. .. The amplicon was then digested with Xba I and Hind III prior to insertion in a pTAC plasmid (Sigma Aldrich, MO, USA) to generate pTAC-SARS-N.

    Polymerase Chain Reaction:

    Article Title: An in vivo cell-based assay for investigating the specific interaction between the SARS-CoV N-protein and its viral RNA packaging sequence.
    Article Snippet: .. Packaging signal sequences for all reporter plasmids are listed in Supplementary Table 2. ii) Construction of protein expression plasmids The N-protein sequence was amplified via PCR from pCMV-SARS-N (Sino Biological Inc., Beijing, China), a vector encoding the full-length human SARS coronavirus, following codon-optimization to increase the level of protein expression in mammalian cells. .. The amplicon was then digested with Xba I and Hind III prior to insertion in a pTAC plasmid (Sigma Aldrich, MO, USA) to generate pTAC-SARS-N.

    Recombinant:

    Article Title: Bioassay Development for Ultrasensitive Detection of Influenza A Nucleoprotein Using Digital ELISA.
    Article Snippet: .. Seven commercially available antibodies were selected for testing their binding affinity toward recombinant influenza A nucleoprotein: one monoclonal (11675-MM03, referred to as SinoM) and one polyclonal (11675-RP01, referred to as SinoP) antibody were purchased from SinoBiological Inc. (China); four monoclonal antibodies (ab110661, ab128193, ab66191, ab67428, in this paper referred to as Ab11, Ab12, Ab66, and Ab67, respectively) were purchased from Abcam plc. (United Kingdom); a polyclonal antibody (IMG-5134A, referred to as ImP) was purchased from Imgenex (United States). .. The selection of antibodies was based on a high variety of possible applications stated in the datasheet (ELISA, Western blot, flow cytometry, ...).

    Plasmid Preparation:

    Article Title: An in vivo cell-based assay for investigating the specific interaction between the SARS-CoV N-protein and its viral RNA packaging sequence.
    Article Snippet: .. Packaging signal sequences for all reporter plasmids are listed in Supplementary Table 2. ii) Construction of protein expression plasmids The N-protein sequence was amplified via PCR from pCMV-SARS-N (Sino Biological Inc., Beijing, China), a vector encoding the full-length human SARS coronavirus, following codon-optimization to increase the level of protein expression in mammalian cells. .. The amplicon was then digested with Xba I and Hind III prior to insertion in a pTAC plasmid (Sigma Aldrich, MO, USA) to generate pTAC-SARS-N.

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    Sino Biological hemagglutinin ha1 ha2
    Hemagglutinin Ha1 Ha2, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hemagglutinin ha1 ha2/product/Sino Biological
    Average 94 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    hemagglutinin ha1 ha2 - by Bioz Stars, 2021-02
    94/100 stars
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