sars cov 2 nucleocapsid antibody  (Sino Biological)


Bioz Verified Symbol Sino Biological is a verified supplier
Bioz Manufacturer Symbol Sino Biological manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95

    Structured Review

    Sino Biological sars cov 2 nucleocapsid antibody
    Mouse-adapted WBP-1 virus RBD enhance interactive affinities with mACE2. (a-d) Modelling of <t>SARS-CoV-2</t> RBD–ACE2 interface. (a) Wuhan-Hu-1 RBD interacts with of human ACE2. (b) WBP-1 RBD (Q493K/Q498H-RBD) interacts human ACE2. (c) Modelling of Wuhan-Hu-1 RBD and mouse ACE2. (d) Modelling of WBP-1 RBD (Q493K/Q498H-RBD) shows interaction with mouse ACE2. (e-l) The binding affinities of the RBD of WBP-1 and mACE2 or hACE2 were determined through BLI experiments. The sensors were dipped in mACE2-hFC and functionalized sensorgrams captured upon incubation of Q493K-RBD (e), Q498H-RBD (f), Q493K/Q498H-RBD (g), and WT-RBD (h) at 6.25 (black), 12.5 (blue), 25 (green), and 50 nM (red). The sensors were dipped in hACE2-hFC and functionalized sensorgrams captured upon incubation of Q493K-RBD (i), Q498H-RBD (j), Q493K/Q498H-RBD (k), and WT-RBD (l) at 50 (black), 100 (blue), 200 (green), and 400 nM (red).
    Sars Cov 2 Nucleocapsid Antibody, supplied by Sino Biological, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sars cov 2 nucleocapsid antibody/product/Sino Biological
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sars cov 2 nucleocapsid antibody - by Bioz Stars, 2021-06
    95/100 stars

    Images

    1) Product Images from "Q493K and Q498H substitutions in Spike promote adaptation of SARS-CoV-2 in mice"

    Article Title: Q493K and Q498H substitutions in Spike promote adaptation of SARS-CoV-2 in mice

    Journal: EBioMedicine

    doi: 10.1016/j.ebiom.2021.103381

    Mouse-adapted WBP-1 virus RBD enhance interactive affinities with mACE2. (a-d) Modelling of SARS-CoV-2 RBD–ACE2 interface. (a) Wuhan-Hu-1 RBD interacts with of human ACE2. (b) WBP-1 RBD (Q493K/Q498H-RBD) interacts human ACE2. (c) Modelling of Wuhan-Hu-1 RBD and mouse ACE2. (d) Modelling of WBP-1 RBD (Q493K/Q498H-RBD) shows interaction with mouse ACE2. (e-l) The binding affinities of the RBD of WBP-1 and mACE2 or hACE2 were determined through BLI experiments. The sensors were dipped in mACE2-hFC and functionalized sensorgrams captured upon incubation of Q493K-RBD (e), Q498H-RBD (f), Q493K/Q498H-RBD (g), and WT-RBD (h) at 6.25 (black), 12.5 (blue), 25 (green), and 50 nM (red). The sensors were dipped in hACE2-hFC and functionalized sensorgrams captured upon incubation of Q493K-RBD (i), Q498H-RBD (j), Q493K/Q498H-RBD (k), and WT-RBD (l) at 50 (black), 100 (blue), 200 (green), and 400 nM (red).
    Figure Legend Snippet: Mouse-adapted WBP-1 virus RBD enhance interactive affinities with mACE2. (a-d) Modelling of SARS-CoV-2 RBD–ACE2 interface. (a) Wuhan-Hu-1 RBD interacts with of human ACE2. (b) WBP-1 RBD (Q493K/Q498H-RBD) interacts human ACE2. (c) Modelling of Wuhan-Hu-1 RBD and mouse ACE2. (d) Modelling of WBP-1 RBD (Q493K/Q498H-RBD) shows interaction with mouse ACE2. (e-l) The binding affinities of the RBD of WBP-1 and mACE2 or hACE2 were determined through BLI experiments. The sensors were dipped in mACE2-hFC and functionalized sensorgrams captured upon incubation of Q493K-RBD (e), Q498H-RBD (f), Q493K/Q498H-RBD (g), and WT-RBD (h) at 6.25 (black), 12.5 (blue), 25 (green), and 50 nM (red). The sensors were dipped in hACE2-hFC and functionalized sensorgrams captured upon incubation of Q493K-RBD (i), Q498H-RBD (j), Q493K/Q498H-RBD (k), and WT-RBD (l) at 50 (black), 100 (blue), 200 (green), and 400 nM (red).

    Techniques Used: Binding Assay, Incubation

    Characterization of mouse-adapted SARS-CoV-2 WBP-1 in mice. (a, b) Groups of mice ( n = 5) were intranasally infected with 10-fold serial dilutions of WBP-1 virus. The infected mice were observed for weight loss and survival for 10 dpi. (c- f) Mice ( n = 6) were intranasally infected with 2 LD 50 of the WBP-1 virus and mice in the control group were mock-infected with DMEM. (c) Viral RNA copies were determined through qRT-PCR at 3 and 5 dpi in the heart, liver, lung, kidney, brain, intestine, trachea, turbinate, and spleen of WBP-1. (d) Viral titers in the lung were determined through plaque assays. (e) Cytokine (IL1β, TNFα, MCP1, and IL10) mRNA levels in the lung were evaluated at 3 and 5 dpi. Statistical significance was analyzed by unpaired Student's t tests. * p
    Figure Legend Snippet: Characterization of mouse-adapted SARS-CoV-2 WBP-1 in mice. (a, b) Groups of mice ( n = 5) were intranasally infected with 10-fold serial dilutions of WBP-1 virus. The infected mice were observed for weight loss and survival for 10 dpi. (c- f) Mice ( n = 6) were intranasally infected with 2 LD 50 of the WBP-1 virus and mice in the control group were mock-infected with DMEM. (c) Viral RNA copies were determined through qRT-PCR at 3 and 5 dpi in the heart, liver, lung, kidney, brain, intestine, trachea, turbinate, and spleen of WBP-1. (d) Viral titers in the lung were determined through plaque assays. (e) Cytokine (IL1β, TNFα, MCP1, and IL10) mRNA levels in the lung were evaluated at 3 and 5 dpi. Statistical significance was analyzed by unpaired Student's t tests. * p

    Techniques Used: Mouse Assay, Infection, Quantitative RT-PCR

    Generation of mouse-adapted SARS-CoV-2 WBP-1. Wild type (WT) Wuhan-Hu-1 was passaged in old and young mice to obtain WBP-1 that was adapted to cause respiratory disease in mice. (a) Hematoxylin and eosin (H E) staining of lung sections from mice infected with virus obtained through different passages (P2, P5, P8, P11) of SARS-CoV-2. Scale bars, 100 μm. (b) Viral copies were detected through qRT-PCR at three days post infection (dpi) in the lungs. (c) Five clonal isolates were obtained from the P11 virus pool by three rounds of plaque purification in Vero cells. The strain #2 that showed a high mortality rate in mice was defined as WBP-1. (d) Location of the mutations and deletion in the genome of WBP-1. WBP-1 obtained five synonymous (purple triangles), five nonsynonymous (red triangles), and a 740 bp deletion (black line). (e) Mutational frequency during SARS-CoV-2 experimental passage in pooled mouse groups. Single nucleotide variants are represented along the genome for the mice of passage 5, passage 8, passage 11 and WBP-1 virus.
    Figure Legend Snippet: Generation of mouse-adapted SARS-CoV-2 WBP-1. Wild type (WT) Wuhan-Hu-1 was passaged in old and young mice to obtain WBP-1 that was adapted to cause respiratory disease in mice. (a) Hematoxylin and eosin (H E) staining of lung sections from mice infected with virus obtained through different passages (P2, P5, P8, P11) of SARS-CoV-2. Scale bars, 100 μm. (b) Viral copies were detected through qRT-PCR at three days post infection (dpi) in the lungs. (c) Five clonal isolates were obtained from the P11 virus pool by three rounds of plaque purification in Vero cells. The strain #2 that showed a high mortality rate in mice was defined as WBP-1. (d) Location of the mutations and deletion in the genome of WBP-1. WBP-1 obtained five synonymous (purple triangles), five nonsynonymous (red triangles), and a 740 bp deletion (black line). (e) Mutational frequency during SARS-CoV-2 experimental passage in pooled mouse groups. Single nucleotide variants are represented along the genome for the mice of passage 5, passage 8, passage 11 and WBP-1 virus.

    Techniques Used: Mouse Assay, Staining, Infection, Quantitative RT-PCR, Purification

    R848 inhibits SARS-CoV-2 in vitro and in vivo . (a) Caco2 cells were treated with R848 (0-20 μM) for 24 h before infection with viruses at a multiplicity of infection (MOI) of 1. Infected cells were further incubated in the R848. Samples were collected at 48 and 72 h post infection (hpi) and viral titers were determined using plaque assays. (b-e) ( n = 10) Mice were intraperitoneally injected with R848 after immediately intranasal infection with the WBP-1 virus. Infected mice were further treated with the R848 or PBS once a day for four consecutive days. Mice were monitored for weight loss (b) and survival (c) for 10 days. (d) Viral loads in mice lung, trachea and turbinate were determined at 5 dpi. (e) Hematoxylin and eosin (H E) staining of lung sections from PBS or R848 treated mice. Scale bars (black), 100 μm. Scale bars (green), 50 μm. (a) and (d), Log transformed data analyzed by Two-way ANOVA followed by Sidak's multiple comparisons. The line represents the mean and error bars represent standard deviation. * p
    Figure Legend Snippet: R848 inhibits SARS-CoV-2 in vitro and in vivo . (a) Caco2 cells were treated with R848 (0-20 μM) for 24 h before infection with viruses at a multiplicity of infection (MOI) of 1. Infected cells were further incubated in the R848. Samples were collected at 48 and 72 h post infection (hpi) and viral titers were determined using plaque assays. (b-e) ( n = 10) Mice were intraperitoneally injected with R848 after immediately intranasal infection with the WBP-1 virus. Infected mice were further treated with the R848 or PBS once a day for four consecutive days. Mice were monitored for weight loss (b) and survival (c) for 10 days. (d) Viral loads in mice lung, trachea and turbinate were determined at 5 dpi. (e) Hematoxylin and eosin (H E) staining of lung sections from PBS or R848 treated mice. Scale bars (black), 100 μm. Scale bars (green), 50 μm. (a) and (d), Log transformed data analyzed by Two-way ANOVA followed by Sidak's multiple comparisons. The line represents the mean and error bars represent standard deviation. * p

    Techniques Used: In Vitro, In Vivo, Infection, Incubation, Mouse Assay, Injection, Staining, Transformation Assay, Standard Deviation

    WBP-1 virus using mouse ACE2 to gain access to cells was determined by immunofluorescence. Hela cells with transfection of empty vector (Flag), hACE2, or mACE2 plasmids were infected with WT Wuhan-Hu-1 or mouse-adapted WBP-1. ACE2 expression was detcted using rabbit anti-ACE2 IgG followed by CoraLite594-conjugated goat anti-rabbit IgG (H+L). SARS-CoV-2 nucleocapsid expression was detected using mouse anti-NP IgG followed by CoraLite488-conjugated Affinipure goat anti-mouse IgG(H+L). Nuclei were stained with DAPI. Scale bars, 200 μm.
    Figure Legend Snippet: WBP-1 virus using mouse ACE2 to gain access to cells was determined by immunofluorescence. Hela cells with transfection of empty vector (Flag), hACE2, or mACE2 plasmids were infected with WT Wuhan-Hu-1 or mouse-adapted WBP-1. ACE2 expression was detcted using rabbit anti-ACE2 IgG followed by CoraLite594-conjugated goat anti-rabbit IgG (H+L). SARS-CoV-2 nucleocapsid expression was detected using mouse anti-NP IgG followed by CoraLite488-conjugated Affinipure goat anti-mouse IgG(H+L). Nuclei were stained with DAPI. Scale bars, 200 μm.

    Techniques Used: Immunofluorescence, Transfection, Plasmid Preparation, Infection, Expressing, Staining

    Related Articles

    Incubation:

    Article Title: Rapid development of SARS-CoV-2 receptor binding domain-conjugated nanoparticle vaccine candidate
    Article Snippet: After incubation for 1 h at 37 °C, 5 % CO2, mixtures were removed and replaced with 100 μL MEM containing 1.2 % carboxymethylcellulose pre-warmed to 37 °C for an additional 24 hours culture. .. Thereafter, cells were fixed with 4% paraformaldehyde and permeabilized with 0.2% Triton X-100 in PBS, and then incubated with rabbit anti-SARS-CoV-2 nucleocapsid protein antibody (Sino Biological, Inc) for 1 hour at ambient temperature, followed by adding of a 1:4000 dilution of HRP-conjugated goat anti-rabbit IgG antibody (Jackson ImmunoResearch Laboratories, Inc. West Grove, PA). ..

    Article Title: SARS-CoV-2–Specific Neutralizing Antibody Responses in Norwegian Health Care Workers After the First Wave of COVID-19 Pandemic: A Prospective Cohort Study
    Article Snippet: The mixtures were incubated for 1 hour at 37°C before transferring to 96-well plates preseeded with Vero cells for 24-hour incubation at 37°C. .. Cells were fixed and permeabilized with methanol and 0.6% H2 O2 and incubated with the anti-SARS-CoV-2 nucleoprotein rabbit monoclonal IgG (Sino Biological), then anti-rabbit biotinylated goat IgG (H+L) (Southern Biotech), extravidin-peroxidase (Sigma-Aldrich) and substrate o-phenylenediamine dihydrochloride (Sigma-Aldrich). .. The MN titer was the reciprocal of the serum dilution giving 50% inhibition of virus infectivity.

    Immunohistochemistry:

    Article Title: Q493K and Q498H substitutions in Spike promote adaptation of SARS-CoV-2 in mice
    Article Snippet: Sections (4 μm thick) were stained with hematoxylin and eosin (H & E) and examined through light microscopy. .. For immunohistochemistry, fixed tissues were paraffin-embedded, sectioned, and stained with rabbit-anti ACE2 (Abcam, ab108252, 1:200), SARS-CoV-2 nucleocapsid antibody (Sino Biological, China, 40588-R001, 1:200). ..

    Article Title: Collapsing Glomerulopathy in a Patient With Coronavirus Disease 2019 (COVID-19)
    Article Snippet: A recent report of postmortem kidney tissue from 6 patients who died of COVID-19 showed acute tubular injury without glomerular abnormalities. .. The SARS-CoV-2 NP antigen was described in renal tubules by immunohistochemical analysis in this report when the renal tissue was reacted with a rabbit monoclonal antibody directed against the SARS-CoV-2 nucleoprotein (Clone ID: 019; Sino Biological, Beijing, China). .. In our laboratory, immunohistochemical analysis of renal tissue using this antibody under numerous conditions showed nonspecific positive staining in the renal parenchyma of all kidneys.

    Staining:

    Article Title: Q493K and Q498H substitutions in Spike promote adaptation of SARS-CoV-2 in mice
    Article Snippet: Sections (4 μm thick) were stained with hematoxylin and eosin (H & E) and examined through light microscopy. .. For immunohistochemistry, fixed tissues were paraffin-embedded, sectioned, and stained with rabbit-anti ACE2 (Abcam, ab108252, 1:200), SARS-CoV-2 nucleocapsid antibody (Sino Biological, China, 40588-R001, 1:200). ..

    Article Title: Recombinant chimpanzee adenovirus AdC7 expressing dimeric tandem-repeat RBD of SARS-CoV-2 spike protein protects mice against COVID-19
    Article Snippet: Tissue sections (4 μm) were deparaffinized in xylene and stained with hematoxylin and eosin (H & E) for pathological examination, such as peribronchiolitis, interstitial pneumonitis and alveolitis. .. Besides, tissue sections were stained with anti-SARS-CoV-2 nucleoprotein antibody (Sino Biological, China) to detect virus infection. ..

    Infection:

    Article Title: Recombinant chimpanzee adenovirus AdC7 expressing dimeric tandem-repeat RBD of SARS-CoV-2 spike protein protects mice against COVID-19
    Article Snippet: Tissue sections (4 μm) were deparaffinized in xylene and stained with hematoxylin and eosin (H & E) for pathological examination, such as peribronchiolitis, interstitial pneumonitis and alveolitis. .. Besides, tissue sections were stained with anti-SARS-CoV-2 nucleoprotein antibody (Sino Biological, China) to detect virus infection. ..

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95
    Sino Biological sars cov 2 nucleocapsid antibody
    Mouse-adapted WBP-1 virus RBD enhance interactive affinities with mACE2. (a-d) Modelling of <t>SARS-CoV-2</t> RBD–ACE2 interface. (a) Wuhan-Hu-1 RBD interacts with of human ACE2. (b) WBP-1 RBD (Q493K/Q498H-RBD) interacts human ACE2. (c) Modelling of Wuhan-Hu-1 RBD and mouse ACE2. (d) Modelling of WBP-1 RBD (Q493K/Q498H-RBD) shows interaction with mouse ACE2. (e-l) The binding affinities of the RBD of WBP-1 and mACE2 or hACE2 were determined through BLI experiments. The sensors were dipped in mACE2-hFC and functionalized sensorgrams captured upon incubation of Q493K-RBD (e), Q498H-RBD (f), Q493K/Q498H-RBD (g), and WT-RBD (h) at 6.25 (black), 12.5 (blue), 25 (green), and 50 nM (red). The sensors were dipped in hACE2-hFC and functionalized sensorgrams captured upon incubation of Q493K-RBD (i), Q498H-RBD (j), Q493K/Q498H-RBD (k), and WT-RBD (l) at 50 (black), 100 (blue), 200 (green), and 400 nM (red).
    Sars Cov 2 Nucleocapsid Antibody, supplied by Sino Biological, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sars cov 2 nucleocapsid antibody/product/Sino Biological
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sars cov 2 nucleocapsid antibody - by Bioz Stars, 2021-06
    95/100 stars
      Buy from Supplier

    Image Search Results


    Mouse-adapted WBP-1 virus RBD enhance interactive affinities with mACE2. (a-d) Modelling of SARS-CoV-2 RBD–ACE2 interface. (a) Wuhan-Hu-1 RBD interacts with of human ACE2. (b) WBP-1 RBD (Q493K/Q498H-RBD) interacts human ACE2. (c) Modelling of Wuhan-Hu-1 RBD and mouse ACE2. (d) Modelling of WBP-1 RBD (Q493K/Q498H-RBD) shows interaction with mouse ACE2. (e-l) The binding affinities of the RBD of WBP-1 and mACE2 or hACE2 were determined through BLI experiments. The sensors were dipped in mACE2-hFC and functionalized sensorgrams captured upon incubation of Q493K-RBD (e), Q498H-RBD (f), Q493K/Q498H-RBD (g), and WT-RBD (h) at 6.25 (black), 12.5 (blue), 25 (green), and 50 nM (red). The sensors were dipped in hACE2-hFC and functionalized sensorgrams captured upon incubation of Q493K-RBD (i), Q498H-RBD (j), Q493K/Q498H-RBD (k), and WT-RBD (l) at 50 (black), 100 (blue), 200 (green), and 400 nM (red).

    Journal: EBioMedicine

    Article Title: Q493K and Q498H substitutions in Spike promote adaptation of SARS-CoV-2 in mice

    doi: 10.1016/j.ebiom.2021.103381

    Figure Lengend Snippet: Mouse-adapted WBP-1 virus RBD enhance interactive affinities with mACE2. (a-d) Modelling of SARS-CoV-2 RBD–ACE2 interface. (a) Wuhan-Hu-1 RBD interacts with of human ACE2. (b) WBP-1 RBD (Q493K/Q498H-RBD) interacts human ACE2. (c) Modelling of Wuhan-Hu-1 RBD and mouse ACE2. (d) Modelling of WBP-1 RBD (Q493K/Q498H-RBD) shows interaction with mouse ACE2. (e-l) The binding affinities of the RBD of WBP-1 and mACE2 or hACE2 were determined through BLI experiments. The sensors were dipped in mACE2-hFC and functionalized sensorgrams captured upon incubation of Q493K-RBD (e), Q498H-RBD (f), Q493K/Q498H-RBD (g), and WT-RBD (h) at 6.25 (black), 12.5 (blue), 25 (green), and 50 nM (red). The sensors were dipped in hACE2-hFC and functionalized sensorgrams captured upon incubation of Q493K-RBD (i), Q498H-RBD (j), Q493K/Q498H-RBD (k), and WT-RBD (l) at 50 (black), 100 (blue), 200 (green), and 400 nM (red).

    Article Snippet: For immunohistochemistry, fixed tissues were paraffin-embedded, sectioned, and stained with rabbit-anti ACE2 (Abcam, ab108252, 1:200), SARS-CoV-2 nucleocapsid antibody (Sino Biological, China, 40588-R001, 1:200).

    Techniques: Binding Assay, Incubation

    Characterization of mouse-adapted SARS-CoV-2 WBP-1 in mice. (a, b) Groups of mice ( n = 5) were intranasally infected with 10-fold serial dilutions of WBP-1 virus. The infected mice were observed for weight loss and survival for 10 dpi. (c- f) Mice ( n = 6) were intranasally infected with 2 LD 50 of the WBP-1 virus and mice in the control group were mock-infected with DMEM. (c) Viral RNA copies were determined through qRT-PCR at 3 and 5 dpi in the heart, liver, lung, kidney, brain, intestine, trachea, turbinate, and spleen of WBP-1. (d) Viral titers in the lung were determined through plaque assays. (e) Cytokine (IL1β, TNFα, MCP1, and IL10) mRNA levels in the lung were evaluated at 3 and 5 dpi. Statistical significance was analyzed by unpaired Student's t tests. * p

    Journal: EBioMedicine

    Article Title: Q493K and Q498H substitutions in Spike promote adaptation of SARS-CoV-2 in mice

    doi: 10.1016/j.ebiom.2021.103381

    Figure Lengend Snippet: Characterization of mouse-adapted SARS-CoV-2 WBP-1 in mice. (a, b) Groups of mice ( n = 5) were intranasally infected with 10-fold serial dilutions of WBP-1 virus. The infected mice were observed for weight loss and survival for 10 dpi. (c- f) Mice ( n = 6) were intranasally infected with 2 LD 50 of the WBP-1 virus and mice in the control group were mock-infected with DMEM. (c) Viral RNA copies were determined through qRT-PCR at 3 and 5 dpi in the heart, liver, lung, kidney, brain, intestine, trachea, turbinate, and spleen of WBP-1. (d) Viral titers in the lung were determined through plaque assays. (e) Cytokine (IL1β, TNFα, MCP1, and IL10) mRNA levels in the lung were evaluated at 3 and 5 dpi. Statistical significance was analyzed by unpaired Student's t tests. * p

    Article Snippet: For immunohistochemistry, fixed tissues were paraffin-embedded, sectioned, and stained with rabbit-anti ACE2 (Abcam, ab108252, 1:200), SARS-CoV-2 nucleocapsid antibody (Sino Biological, China, 40588-R001, 1:200).

    Techniques: Mouse Assay, Infection, Quantitative RT-PCR

    Generation of mouse-adapted SARS-CoV-2 WBP-1. Wild type (WT) Wuhan-Hu-1 was passaged in old and young mice to obtain WBP-1 that was adapted to cause respiratory disease in mice. (a) Hematoxylin and eosin (H E) staining of lung sections from mice infected with virus obtained through different passages (P2, P5, P8, P11) of SARS-CoV-2. Scale bars, 100 μm. (b) Viral copies were detected through qRT-PCR at three days post infection (dpi) in the lungs. (c) Five clonal isolates were obtained from the P11 virus pool by three rounds of plaque purification in Vero cells. The strain #2 that showed a high mortality rate in mice was defined as WBP-1. (d) Location of the mutations and deletion in the genome of WBP-1. WBP-1 obtained five synonymous (purple triangles), five nonsynonymous (red triangles), and a 740 bp deletion (black line). (e) Mutational frequency during SARS-CoV-2 experimental passage in pooled mouse groups. Single nucleotide variants are represented along the genome for the mice of passage 5, passage 8, passage 11 and WBP-1 virus.

    Journal: EBioMedicine

    Article Title: Q493K and Q498H substitutions in Spike promote adaptation of SARS-CoV-2 in mice

    doi: 10.1016/j.ebiom.2021.103381

    Figure Lengend Snippet: Generation of mouse-adapted SARS-CoV-2 WBP-1. Wild type (WT) Wuhan-Hu-1 was passaged in old and young mice to obtain WBP-1 that was adapted to cause respiratory disease in mice. (a) Hematoxylin and eosin (H E) staining of lung sections from mice infected with virus obtained through different passages (P2, P5, P8, P11) of SARS-CoV-2. Scale bars, 100 μm. (b) Viral copies were detected through qRT-PCR at three days post infection (dpi) in the lungs. (c) Five clonal isolates were obtained from the P11 virus pool by three rounds of plaque purification in Vero cells. The strain #2 that showed a high mortality rate in mice was defined as WBP-1. (d) Location of the mutations and deletion in the genome of WBP-1. WBP-1 obtained five synonymous (purple triangles), five nonsynonymous (red triangles), and a 740 bp deletion (black line). (e) Mutational frequency during SARS-CoV-2 experimental passage in pooled mouse groups. Single nucleotide variants are represented along the genome for the mice of passage 5, passage 8, passage 11 and WBP-1 virus.

    Article Snippet: For immunohistochemistry, fixed tissues were paraffin-embedded, sectioned, and stained with rabbit-anti ACE2 (Abcam, ab108252, 1:200), SARS-CoV-2 nucleocapsid antibody (Sino Biological, China, 40588-R001, 1:200).

    Techniques: Mouse Assay, Staining, Infection, Quantitative RT-PCR, Purification

    R848 inhibits SARS-CoV-2 in vitro and in vivo . (a) Caco2 cells were treated with R848 (0-20 μM) for 24 h before infection with viruses at a multiplicity of infection (MOI) of 1. Infected cells were further incubated in the R848. Samples were collected at 48 and 72 h post infection (hpi) and viral titers were determined using plaque assays. (b-e) ( n = 10) Mice were intraperitoneally injected with R848 after immediately intranasal infection with the WBP-1 virus. Infected mice were further treated with the R848 or PBS once a day for four consecutive days. Mice were monitored for weight loss (b) and survival (c) for 10 days. (d) Viral loads in mice lung, trachea and turbinate were determined at 5 dpi. (e) Hematoxylin and eosin (H E) staining of lung sections from PBS or R848 treated mice. Scale bars (black), 100 μm. Scale bars (green), 50 μm. (a) and (d), Log transformed data analyzed by Two-way ANOVA followed by Sidak's multiple comparisons. The line represents the mean and error bars represent standard deviation. * p

    Journal: EBioMedicine

    Article Title: Q493K and Q498H substitutions in Spike promote adaptation of SARS-CoV-2 in mice

    doi: 10.1016/j.ebiom.2021.103381

    Figure Lengend Snippet: R848 inhibits SARS-CoV-2 in vitro and in vivo . (a) Caco2 cells were treated with R848 (0-20 μM) for 24 h before infection with viruses at a multiplicity of infection (MOI) of 1. Infected cells were further incubated in the R848. Samples were collected at 48 and 72 h post infection (hpi) and viral titers were determined using plaque assays. (b-e) ( n = 10) Mice were intraperitoneally injected with R848 after immediately intranasal infection with the WBP-1 virus. Infected mice were further treated with the R848 or PBS once a day for four consecutive days. Mice were monitored for weight loss (b) and survival (c) for 10 days. (d) Viral loads in mice lung, trachea and turbinate were determined at 5 dpi. (e) Hematoxylin and eosin (H E) staining of lung sections from PBS or R848 treated mice. Scale bars (black), 100 μm. Scale bars (green), 50 μm. (a) and (d), Log transformed data analyzed by Two-way ANOVA followed by Sidak's multiple comparisons. The line represents the mean and error bars represent standard deviation. * p

    Article Snippet: For immunohistochemistry, fixed tissues were paraffin-embedded, sectioned, and stained with rabbit-anti ACE2 (Abcam, ab108252, 1:200), SARS-CoV-2 nucleocapsid antibody (Sino Biological, China, 40588-R001, 1:200).

    Techniques: In Vitro, In Vivo, Infection, Incubation, Mouse Assay, Injection, Staining, Transformation Assay, Standard Deviation

    WBP-1 virus using mouse ACE2 to gain access to cells was determined by immunofluorescence. Hela cells with transfection of empty vector (Flag), hACE2, or mACE2 plasmids were infected with WT Wuhan-Hu-1 or mouse-adapted WBP-1. ACE2 expression was detcted using rabbit anti-ACE2 IgG followed by CoraLite594-conjugated goat anti-rabbit IgG (H+L). SARS-CoV-2 nucleocapsid expression was detected using mouse anti-NP IgG followed by CoraLite488-conjugated Affinipure goat anti-mouse IgG(H+L). Nuclei were stained with DAPI. Scale bars, 200 μm.

    Journal: EBioMedicine

    Article Title: Q493K and Q498H substitutions in Spike promote adaptation of SARS-CoV-2 in mice

    doi: 10.1016/j.ebiom.2021.103381

    Figure Lengend Snippet: WBP-1 virus using mouse ACE2 to gain access to cells was determined by immunofluorescence. Hela cells with transfection of empty vector (Flag), hACE2, or mACE2 plasmids were infected with WT Wuhan-Hu-1 or mouse-adapted WBP-1. ACE2 expression was detcted using rabbit anti-ACE2 IgG followed by CoraLite594-conjugated goat anti-rabbit IgG (H+L). SARS-CoV-2 nucleocapsid expression was detected using mouse anti-NP IgG followed by CoraLite488-conjugated Affinipure goat anti-mouse IgG(H+L). Nuclei were stained with DAPI. Scale bars, 200 μm.

    Article Snippet: For immunohistochemistry, fixed tissues were paraffin-embedded, sectioned, and stained with rabbit-anti ACE2 (Abcam, ab108252, 1:200), SARS-CoV-2 nucleocapsid antibody (Sino Biological, China, 40588-R001, 1:200).

    Techniques: Immunofluorescence, Transfection, Plasmid Preparation, Infection, Expressing, Staining