sars cov 2 spike (Sino Biological)


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Sars Cov 2 Spike, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 0 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Major role of IgM in the neutralizing activity of convalescent plasma against SARS-CoV-2"
Article Title: Major role of IgM in the neutralizing activity of convalescent plasma against SARS-CoV-2
Journal: Cell Reports
doi: 10.1016/j.celrep.2021.108790

Figure Legend Snippet: IgM, IgA, and IgG depletion in plasma samples from convalescent donors (A–C) Efficacy of the specific isotype depletion assessed by ELISA for total IgM, IgA, and IgG. All plasma samples were diluted 5-fold before depletion. (A) IgM concentration in non-depleted, IgM-depleted, IgA-depleted, and IgG-depleted plasma samples, measured with an anti-human IgM (μ-chain specific) as the capture antibody. (B) IgA concentration measured on the same plasma samples with an anti-human IgA (α-chain specific). (C) IgG concentration measured with anti-human an IgG (γ-chain specific). (D–G) Efficacy of SARS-CoV-2-specific antibody depletion as assessed by SARS-CoV-2 RBD ELISA. (D) Level of total (pan-Ig) anti-SARS-CoV-2, RBD-specific antibodies in non-depleted, IgM-depleted, IgA-depleted, and IgG-depleted plasma samples. (E) Level of IgM-specific anti-RBD. (F) Level of IgA-specific anti-RBD. (G) Level of IgG-specific anti-RBD. (H–K) Efficacy of full S glycoprotein-specific antibody depletion measured by flow cytometry. (H) Level of total (pan-Ig) anti-SARS-CoV-2 S-specific antibodies in non-depleted, IgM-depleted, IgA-depleted, and IgG-depleted plasma samples. (I) Level of IgM-specific anti-S. (J) Level of IgA-specific anti-S. (K) Level of IgG-specific anti-S. Red dashed lines represent the average signal given by negative controls taken from non-infected patients. Asterisks indicate the level of statistical significance obtained by a Dunn’s test. ∗∗∗∗ p
Techniques Used: Enzyme-linked Immunosorbent Assay, Concentration Assay, Flow Cytometry, Infection

Figure Legend Snippet: Role of IgM, IgA, and IgG in neutralization (A) Comparison of the SARS-CoV-2 pseudoviral inhibitory dilution (ID 50 ) of all plasma samples. (B–D) ID 50 of plasma from each convalescent donor before and after IgM (B), IgA (C), and IgG (D) depletion. (E) Fold decrease (isotype-depleted versus non-depleted plasma) in ID 50 measured by SARS-CoV-2 pseudoviral particle neutralization. (F and G) Microneutralization assay with infectious wild-type SARS-CoV-2 performed on non-depleted and isotype-depleted plasma from 10 donors. Mean percentage of infection (F) and ID 50 observed from plasma from the 10 donors (G). (H) ID 50 obtained with the pseudoviral particle neutralization assay for the samples in (F)–(G). Asterisks indicate the level of statistical significance obtained by a Wilcoxon signed rank test. n.s., not significant. ∗ p
Techniques Used: Neutralization, Microneutralization Assay, Infection
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