sars cov 2 spike  (Sino Biological)


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    Sino Biological sars cov 2 spike
    IgM, IgA, and IgG depletion in plasma samples from convalescent donors (A–C) Efficacy of the specific isotype depletion assessed by ELISA for total IgM, IgA, and IgG. All plasma samples were diluted 5-fold before depletion. (A) IgM concentration in non-depleted, IgM-depleted, IgA-depleted, and IgG-depleted plasma samples, measured with an anti-human IgM (μ-chain specific) as the capture antibody. (B) IgA concentration measured on the same plasma samples with an anti-human IgA (α-chain specific). (C) IgG concentration measured with anti-human an IgG (γ-chain specific). (D–G) Efficacy of <t>SARS-CoV-2-specific</t> antibody depletion as assessed by SARS-CoV-2 RBD ELISA. (D) Level of total (pan-Ig) anti-SARS-CoV-2, RBD-specific antibodies in non-depleted, IgM-depleted, IgA-depleted, and IgG-depleted plasma samples. (E) Level of IgM-specific anti-RBD. (F) Level of IgA-specific anti-RBD. (G) Level of IgG-specific anti-RBD. (H–K) Efficacy of full S glycoprotein-specific antibody depletion measured by flow cytometry. (H) Level of total (pan-Ig) anti-SARS-CoV-2 S-specific antibodies in non-depleted, IgM-depleted, IgA-depleted, and IgG-depleted plasma samples. (I) Level of IgM-specific anti-S. (J) Level of IgA-specific anti-S. (K) Level of IgG-specific anti-S. Red dashed lines represent the average signal given by negative controls taken from non-infected patients. Asterisks indicate the level of statistical significance obtained by a Dunn’s test. ∗∗∗∗ p
    Sars Cov 2 Spike, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 0 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sars cov 2 spike/product/Sino Biological
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sars cov 2 spike - by Bioz Stars, 2021-02
    93/100 stars

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    1) Product Images from "Major role of IgM in the neutralizing activity of convalescent plasma against SARS-CoV-2"

    Article Title: Major role of IgM in the neutralizing activity of convalescent plasma against SARS-CoV-2

    Journal: Cell Reports

    doi: 10.1016/j.celrep.2021.108790

    IgM, IgA, and IgG depletion in plasma samples from convalescent donors (A–C) Efficacy of the specific isotype depletion assessed by ELISA for total IgM, IgA, and IgG. All plasma samples were diluted 5-fold before depletion. (A) IgM concentration in non-depleted, IgM-depleted, IgA-depleted, and IgG-depleted plasma samples, measured with an anti-human IgM (μ-chain specific) as the capture antibody. (B) IgA concentration measured on the same plasma samples with an anti-human IgA (α-chain specific). (C) IgG concentration measured with anti-human an IgG (γ-chain specific). (D–G) Efficacy of SARS-CoV-2-specific antibody depletion as assessed by SARS-CoV-2 RBD ELISA. (D) Level of total (pan-Ig) anti-SARS-CoV-2, RBD-specific antibodies in non-depleted, IgM-depleted, IgA-depleted, and IgG-depleted plasma samples. (E) Level of IgM-specific anti-RBD. (F) Level of IgA-specific anti-RBD. (G) Level of IgG-specific anti-RBD. (H–K) Efficacy of full S glycoprotein-specific antibody depletion measured by flow cytometry. (H) Level of total (pan-Ig) anti-SARS-CoV-2 S-specific antibodies in non-depleted, IgM-depleted, IgA-depleted, and IgG-depleted plasma samples. (I) Level of IgM-specific anti-S. (J) Level of IgA-specific anti-S. (K) Level of IgG-specific anti-S. Red dashed lines represent the average signal given by negative controls taken from non-infected patients. Asterisks indicate the level of statistical significance obtained by a Dunn’s test. ∗∗∗∗ p
    Figure Legend Snippet: IgM, IgA, and IgG depletion in plasma samples from convalescent donors (A–C) Efficacy of the specific isotype depletion assessed by ELISA for total IgM, IgA, and IgG. All plasma samples were diluted 5-fold before depletion. (A) IgM concentration in non-depleted, IgM-depleted, IgA-depleted, and IgG-depleted plasma samples, measured with an anti-human IgM (μ-chain specific) as the capture antibody. (B) IgA concentration measured on the same plasma samples with an anti-human IgA (α-chain specific). (C) IgG concentration measured with anti-human an IgG (γ-chain specific). (D–G) Efficacy of SARS-CoV-2-specific antibody depletion as assessed by SARS-CoV-2 RBD ELISA. (D) Level of total (pan-Ig) anti-SARS-CoV-2, RBD-specific antibodies in non-depleted, IgM-depleted, IgA-depleted, and IgG-depleted plasma samples. (E) Level of IgM-specific anti-RBD. (F) Level of IgA-specific anti-RBD. (G) Level of IgG-specific anti-RBD. (H–K) Efficacy of full S glycoprotein-specific antibody depletion measured by flow cytometry. (H) Level of total (pan-Ig) anti-SARS-CoV-2 S-specific antibodies in non-depleted, IgM-depleted, IgA-depleted, and IgG-depleted plasma samples. (I) Level of IgM-specific anti-S. (J) Level of IgA-specific anti-S. (K) Level of IgG-specific anti-S. Red dashed lines represent the average signal given by negative controls taken from non-infected patients. Asterisks indicate the level of statistical significance obtained by a Dunn’s test. ∗∗∗∗ p

    Techniques Used: Enzyme-linked Immunosorbent Assay, Concentration Assay, Flow Cytometry, Infection

    Role of IgM, IgA, and IgG in neutralization (A) Comparison of the SARS-CoV-2 pseudoviral inhibitory dilution (ID 50 ) of all plasma samples. (B–D) ID 50 of plasma from each convalescent donor before and after IgM (B), IgA (C), and IgG (D) depletion. (E) Fold decrease (isotype-depleted versus non-depleted plasma) in ID 50 measured by SARS-CoV-2 pseudoviral particle neutralization. (F and G) Microneutralization assay with infectious wild-type SARS-CoV-2 performed on non-depleted and isotype-depleted plasma from 10 donors. Mean percentage of infection (F) and ID 50 observed from plasma from the 10 donors (G). (H) ID 50 obtained with the pseudoviral particle neutralization assay for the samples in (F)–(G). Asterisks indicate the level of statistical significance obtained by a Wilcoxon signed rank test. n.s., not significant. ∗ p
    Figure Legend Snippet: Role of IgM, IgA, and IgG in neutralization (A) Comparison of the SARS-CoV-2 pseudoviral inhibitory dilution (ID 50 ) of all plasma samples. (B–D) ID 50 of plasma from each convalescent donor before and after IgM (B), IgA (C), and IgG (D) depletion. (E) Fold decrease (isotype-depleted versus non-depleted plasma) in ID 50 measured by SARS-CoV-2 pseudoviral particle neutralization. (F and G) Microneutralization assay with infectious wild-type SARS-CoV-2 performed on non-depleted and isotype-depleted plasma from 10 donors. Mean percentage of infection (F) and ID 50 observed from plasma from the 10 donors (G). (H) ID 50 obtained with the pseudoviral particle neutralization assay for the samples in (F)–(G). Asterisks indicate the level of statistical significance obtained by a Wilcoxon signed rank test. n.s., not significant. ∗ p

    Techniques Used: Neutralization, Microneutralization Assay, Infection

    Related Articles

    Incubation:

    Article Title: In vitro efficacy of Artemisinin-based treatments against SARS-CoV-2
    Article Snippet: .. Following removal of blocking solution, plates were incubated with primary antibody SARS-CoV-2 spike chimeric monoclonal antibody (Sino Biological #40150-D004, Beijing, China) diluted 1:5000 in PBS containing 1% bovine serum albumin and 0.2% skim milk powder overnight at 4 C. Following two washes with PBS containing 0.1% Tween-20, plates were incubated with secondary antibody F(ab’)2-Goat anti-Human IgG Fc Cross-Adsorbed Secondary Antibody, HRP (Invitrogen #A24476, Carlsbad, CA, USA) or Goat F(ab’)2 Anti-Human IgG - Fc (HRP), pre-adsorbed (Abcamab#98595, Cambridge, UK) diluted 1:2000 in PBS containing 1% bovine serum albumin and 0.2% skim milk powder for 2 h. Following two washes with PBS containing 0.1% Tween-20, SARS-CoV-2 spike glycoprotein was visualized using DAB substrate (Immunologic # BS04-110, Duiven, Netherlands). .. Spike protein positive cells were counted automatically using an ImmunoSpot series 5 UV analyzer (CTL Europe GmbH, Bonn, Germany) as described., , The average count of 12 noninfected nontreated control wells, which was usually < 50, was subtracted from the count of each infected well.

    Article Title: Efficient culture of SARS-CoV-2 in human hepatoma cells enhances viability of the virus in human lung cancer cell lines permitting the screening of antiviral compounds
    Article Snippet: .. Cells were then incubated with primary antibody SARS-CoV-2 spike chimeric monoclonal antibody (Sino Biological #40150-D004) diluted 1:5000 in PBS with 1% bovine serum albumin and 0.2% skim milk powder (PBSK) overnight at 4 ℃. .. Afterwards, cells were washed twice with PBST followed by 1-hour incubation with secondary antibody F(ab’)2-Goat anti-Human IgG Fc Cross-Adsorbed Secondary Antibody, HRP (Invitrogen #A24476) diluted 1:2000 in PBSK.

    Article Title: Hepatitis C Virus Protease Inhibitors Show Differential Efficacy and Interactions with Remdesivir for Treatment of SARS-CoV-2 in Vitro
    Article Snippet: .. Next, plates were emptied and incubated with primary antibody SARS-CoV-2 spike chimeric monoclonal antibody (Sino Biological #40150-D004, Beijing, China) diluted 1:5,000 in PBSK for 2 hours at room temperature. .. Then plates were washed 2 times with PBS-tween and incubated for 1 hour at room temperature with secondary antibody F(ab’)2-Goat anti-human IgG Fc Cross-Adsorbed Secondary Antibody, HRP (Invitrogen#A24476, Carlsbad, CA, USA) or Goat F(ab’)2 Anti-Human IgG – Fc (HRP), preadsorbed (Abcamab#98595, Cambridge, UK), diluted 1:2,000 in PBSK.

    Expressing:

    Article Title: A human coronavirus evolves antigenically to escape antibody immunity
    Article Snippet: .. The only modifications for this study are that we used the plasmids expressing the 229E spike described above rather than plasmids expressing the SARS-CoV-2 spike, and that after producing the pseudotyped lentiviral particles we infected them into target cells engineered to be infectable by the 229E spike. .. Specifically, the 229E spike binds to human aminopeptidase N (APN) to initiate viral entry ( ).

    Infection:

    Article Title: A human coronavirus evolves antigenically to escape antibody immunity
    Article Snippet: .. The only modifications for this study are that we used the plasmids expressing the 229E spike described above rather than plasmids expressing the SARS-CoV-2 spike, and that after producing the pseudotyped lentiviral particles we infected them into target cells engineered to be infectable by the 229E spike. .. Specifically, the 229E spike binds to human aminopeptidase N (APN) to initiate viral entry ( ).

    Blocking Assay:

    Article Title: In vitro efficacy of Artemisinin-based treatments against SARS-CoV-2
    Article Snippet: .. Following removal of blocking solution, plates were incubated with primary antibody SARS-CoV-2 spike chimeric monoclonal antibody (Sino Biological #40150-D004, Beijing, China) diluted 1:5000 in PBS containing 1% bovine serum albumin and 0.2% skim milk powder overnight at 4 C. Following two washes with PBS containing 0.1% Tween-20, plates were incubated with secondary antibody F(ab’)2-Goat anti-Human IgG Fc Cross-Adsorbed Secondary Antibody, HRP (Invitrogen #A24476, Carlsbad, CA, USA) or Goat F(ab’)2 Anti-Human IgG - Fc (HRP), pre-adsorbed (Abcamab#98595, Cambridge, UK) diluted 1:2000 in PBS containing 1% bovine serum albumin and 0.2% skim milk powder for 2 h. Following two washes with PBS containing 0.1% Tween-20, SARS-CoV-2 spike glycoprotein was visualized using DAB substrate (Immunologic # BS04-110, Duiven, Netherlands). .. Spike protein positive cells were counted automatically using an ImmunoSpot series 5 UV analyzer (CTL Europe GmbH, Bonn, Germany) as described., , The average count of 12 noninfected nontreated control wells, which was usually < 50, was subtracted from the count of each infected well.

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    Sino Biological sars cov sars cov 2 spike antibody chimeric mab
    Sars Cov Sars Cov 2 Spike Antibody Chimeric Mab, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sars cov sars cov 2 spike antibody chimeric mab/product/Sino Biological
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sars cov sars cov 2 spike antibody chimeric mab - by Bioz Stars, 2021-02
    93/100 stars
      Buy from Supplier

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    Sino Biological sars cov 2 spike
    IgM, IgA, and IgG depletion in plasma samples from convalescent donors (A–C) Efficacy of the specific isotype depletion assessed by ELISA for total IgM, IgA, and IgG. All plasma samples were diluted 5-fold before depletion. (A) IgM concentration in non-depleted, IgM-depleted, IgA-depleted, and IgG-depleted plasma samples, measured with an anti-human IgM (μ-chain specific) as the capture antibody. (B) IgA concentration measured on the same plasma samples with an anti-human IgA (α-chain specific). (C) IgG concentration measured with anti-human an IgG (γ-chain specific). (D–G) Efficacy of <t>SARS-CoV-2-specific</t> antibody depletion as assessed by SARS-CoV-2 RBD ELISA. (D) Level of total (pan-Ig) anti-SARS-CoV-2, RBD-specific antibodies in non-depleted, IgM-depleted, IgA-depleted, and IgG-depleted plasma samples. (E) Level of IgM-specific anti-RBD. (F) Level of IgA-specific anti-RBD. (G) Level of IgG-specific anti-RBD. (H–K) Efficacy of full S glycoprotein-specific antibody depletion measured by flow cytometry. (H) Level of total (pan-Ig) anti-SARS-CoV-2 S-specific antibodies in non-depleted, IgM-depleted, IgA-depleted, and IgG-depleted plasma samples. (I) Level of IgM-specific anti-S. (J) Level of IgA-specific anti-S. (K) Level of IgG-specific anti-S. Red dashed lines represent the average signal given by negative controls taken from non-infected patients. Asterisks indicate the level of statistical significance obtained by a Dunn’s test. ∗∗∗∗ p
    Sars Cov 2 Spike, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 0 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sars cov 2 spike/product/Sino Biological
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sars cov 2 spike - by Bioz Stars, 2021-02
    93/100 stars
      Buy from Supplier

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    IgM, IgA, and IgG depletion in plasma samples from convalescent donors (A–C) Efficacy of the specific isotype depletion assessed by ELISA for total IgM, IgA, and IgG. All plasma samples were diluted 5-fold before depletion. (A) IgM concentration in non-depleted, IgM-depleted, IgA-depleted, and IgG-depleted plasma samples, measured with an anti-human IgM (μ-chain specific) as the capture antibody. (B) IgA concentration measured on the same plasma samples with an anti-human IgA (α-chain specific). (C) IgG concentration measured with anti-human an IgG (γ-chain specific). (D–G) Efficacy of SARS-CoV-2-specific antibody depletion as assessed by SARS-CoV-2 RBD ELISA. (D) Level of total (pan-Ig) anti-SARS-CoV-2, RBD-specific antibodies in non-depleted, IgM-depleted, IgA-depleted, and IgG-depleted plasma samples. (E) Level of IgM-specific anti-RBD. (F) Level of IgA-specific anti-RBD. (G) Level of IgG-specific anti-RBD. (H–K) Efficacy of full S glycoprotein-specific antibody depletion measured by flow cytometry. (H) Level of total (pan-Ig) anti-SARS-CoV-2 S-specific antibodies in non-depleted, IgM-depleted, IgA-depleted, and IgG-depleted plasma samples. (I) Level of IgM-specific anti-S. (J) Level of IgA-specific anti-S. (K) Level of IgG-specific anti-S. Red dashed lines represent the average signal given by negative controls taken from non-infected patients. Asterisks indicate the level of statistical significance obtained by a Dunn’s test. ∗∗∗∗ p

    Journal: Cell Reports

    Article Title: Major role of IgM in the neutralizing activity of convalescent plasma against SARS-CoV-2

    doi: 10.1016/j.celrep.2021.108790

    Figure Lengend Snippet: IgM, IgA, and IgG depletion in plasma samples from convalescent donors (A–C) Efficacy of the specific isotype depletion assessed by ELISA for total IgM, IgA, and IgG. All plasma samples were diluted 5-fold before depletion. (A) IgM concentration in non-depleted, IgM-depleted, IgA-depleted, and IgG-depleted plasma samples, measured with an anti-human IgM (μ-chain specific) as the capture antibody. (B) IgA concentration measured on the same plasma samples with an anti-human IgA (α-chain specific). (C) IgG concentration measured with anti-human an IgG (γ-chain specific). (D–G) Efficacy of SARS-CoV-2-specific antibody depletion as assessed by SARS-CoV-2 RBD ELISA. (D) Level of total (pan-Ig) anti-SARS-CoV-2, RBD-specific antibodies in non-depleted, IgM-depleted, IgA-depleted, and IgG-depleted plasma samples. (E) Level of IgM-specific anti-RBD. (F) Level of IgA-specific anti-RBD. (G) Level of IgG-specific anti-RBD. (H–K) Efficacy of full S glycoprotein-specific antibody depletion measured by flow cytometry. (H) Level of total (pan-Ig) anti-SARS-CoV-2 S-specific antibodies in non-depleted, IgM-depleted, IgA-depleted, and IgG-depleted plasma samples. (I) Level of IgM-specific anti-S. (J) Level of IgA-specific anti-S. (K) Level of IgG-specific anti-S. Red dashed lines represent the average signal given by negative controls taken from non-infected patients. Asterisks indicate the level of statistical significance obtained by a Dunn’s test. ∗∗∗∗ p

    Article Snippet: For the generation of 293T cells stably expressing SARS-CoV-2 Spike the same technique than previously described has been used ( ): VSV-G pseudotyped lentivirus packaging the SARS-CoV-2 Spike gene was produced in 293T using a third-generation lentiviral vector system.

    Techniques: Enzyme-linked Immunosorbent Assay, Concentration Assay, Flow Cytometry, Infection

    Role of IgM, IgA, and IgG in neutralization (A) Comparison of the SARS-CoV-2 pseudoviral inhibitory dilution (ID 50 ) of all plasma samples. (B–D) ID 50 of plasma from each convalescent donor before and after IgM (B), IgA (C), and IgG (D) depletion. (E) Fold decrease (isotype-depleted versus non-depleted plasma) in ID 50 measured by SARS-CoV-2 pseudoviral particle neutralization. (F and G) Microneutralization assay with infectious wild-type SARS-CoV-2 performed on non-depleted and isotype-depleted plasma from 10 donors. Mean percentage of infection (F) and ID 50 observed from plasma from the 10 donors (G). (H) ID 50 obtained with the pseudoviral particle neutralization assay for the samples in (F)–(G). Asterisks indicate the level of statistical significance obtained by a Wilcoxon signed rank test. n.s., not significant. ∗ p

    Journal: Cell Reports

    Article Title: Major role of IgM in the neutralizing activity of convalescent plasma against SARS-CoV-2

    doi: 10.1016/j.celrep.2021.108790

    Figure Lengend Snippet: Role of IgM, IgA, and IgG in neutralization (A) Comparison of the SARS-CoV-2 pseudoviral inhibitory dilution (ID 50 ) of all plasma samples. (B–D) ID 50 of plasma from each convalescent donor before and after IgM (B), IgA (C), and IgG (D) depletion. (E) Fold decrease (isotype-depleted versus non-depleted plasma) in ID 50 measured by SARS-CoV-2 pseudoviral particle neutralization. (F and G) Microneutralization assay with infectious wild-type SARS-CoV-2 performed on non-depleted and isotype-depleted plasma from 10 donors. Mean percentage of infection (F) and ID 50 observed from plasma from the 10 donors (G). (H) ID 50 obtained with the pseudoviral particle neutralization assay for the samples in (F)–(G). Asterisks indicate the level of statistical significance obtained by a Wilcoxon signed rank test. n.s., not significant. ∗ p

    Article Snippet: For the generation of 293T cells stably expressing SARS-CoV-2 Spike the same technique than previously described has been used ( ): VSV-G pseudotyped lentivirus packaging the SARS-CoV-2 Spike gene was produced in 293T using a third-generation lentiviral vector system.

    Techniques: Neutralization, Microneutralization Assay, Infection