sars cov spike s1 subunit antibody mouse mab  (Sino Biological)


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    Name:
    SARS CoV Spike S1 Subunit Antibody Mouse MAb
    Description:
    This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified recombinant SARS CoV Spike S1 Subunit Catalog 40150 V08B1 AAX16192 1 Met1 Arg667 The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography
    Catalog Number:
    40150-mm02
    Product Aliases:
    Anti-coronavirus s1 Antibody, Anti-coronavirus s2 Antibody, Anti-coronavirus spike Antibody, Anti-cov spike Antibody, Anti-ncov RBD Antibody, Anti-ncov s1 Antibody, Anti-ncov s2 Antibody, Anti-ncov spike Antibody, Anti-novel coronavirus RBD Antibody, Anti-novel coronavirus s1 Antibody, Anti-novel coronavirus s2 Antibody, Anti-novel coronavirus spike Antibody, Anti-RBD Antibody, Anti-S1 Antibody, Anti-s2 Antibody, Anti-Spike RBD Antibody
    Price:
    None
    Applications:
    ELISA
    Host:
    Mouse
    Immunogen:
    Recombinant SARS-CoV Spike S1 Subunit Protein (Catalog#40150-V08B1)
    Category:
    Primary Antibody
    Antibody Type:
    MAb
    Isotype:
    Mouse IgG1
    Reactivity:
    SARS
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    Structured Review

    Sino Biological sars cov spike s1 subunit antibody mouse mab
    Pellets of HEK293 cells transfected with SARS-CoV2: S1 and S2 subunit of spike protein, nucleoprotein and untransfected cells. A–D, Hematoxylin eosin stain/H  E. E–H, mAb FIPV3-20 to nucleoprotein, no immunolabeling of any pellet. I–L, mAb 019, intense immunostaining of all cell pellets. M–P, mAb 1A9, exclusive immunoreactivity of HEK293 cells transfected with spike protein S2 subunit. Q–T, mAb 001, homogeneous staining of HEK293 cells expressing nucleoprotein. U–X. In situ hybridization with probe to S1 subunit positive in corresponding HEK293 cells.
    This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified recombinant SARS CoV Spike S1 Subunit Catalog 40150 V08B1 AAX16192 1 Met1 Arg667 The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography
    https://www.bioz.com/result/sars cov spike s1 subunit antibody mouse mab/product/Sino Biological
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    sars cov spike s1 subunit antibody mouse mab - by Bioz Stars, 2021-02
    93/100 stars

    Images

    1) Product Images from "Identification of Immunohistochemical Reagents for In Situ Protein Expression Analysis of Coronavirus-associated Changes in Human Tissues"

    Article Title: Identification of Immunohistochemical Reagents for In Situ Protein Expression Analysis of Coronavirus-associated Changes in Human Tissues

    Journal: Applied Immunohistochemistry & Molecular Morphology

    doi: 10.1097/PAI.0000000000000878

    Pellets of HEK293 cells transfected with SARS-CoV2: S1 and S2 subunit of spike protein, nucleoprotein and untransfected cells. A–D, Hematoxylin eosin stain/H  E. E–H, mAb FIPV3-20 to nucleoprotein, no immunolabeling of any pellet. I–L, mAb 019, intense immunostaining of all cell pellets. M–P, mAb 1A9, exclusive immunoreactivity of HEK293 cells transfected with spike protein S2 subunit. Q–T, mAb 001, homogeneous staining of HEK293 cells expressing nucleoprotein. U–X. In situ hybridization with probe to S1 subunit positive in corresponding HEK293 cells.
    Figure Legend Snippet: Pellets of HEK293 cells transfected with SARS-CoV2: S1 and S2 subunit of spike protein, nucleoprotein and untransfected cells. A–D, Hematoxylin eosin stain/H E. E–H, mAb FIPV3-20 to nucleoprotein, no immunolabeling of any pellet. I–L, mAb 019, intense immunostaining of all cell pellets. M–P, mAb 1A9, exclusive immunoreactivity of HEK293 cells transfected with spike protein S2 subunit. Q–T, mAb 001, homogeneous staining of HEK293 cells expressing nucleoprotein. U–X. In situ hybridization with probe to S1 subunit positive in corresponding HEK293 cells.

    Techniques Used: Transfection, Staining, Immunolabeling, Immunostaining, Expressing, In Situ Hybridization

    2) Product Images from "Single-cell analyses reveal SARS-CoV-2 interference with intrinsic immune response in the human gut"

    Article Title: Single-cell analyses reveal SARS-CoV-2 interference with intrinsic immune response in the human gut

    Journal: bioRxiv

    doi: 10.1101/2020.10.21.348854

    SARS-CoV-2 cell tropism in human colon- and ileum-derived organoids. A. UMAP visualization of scRNAseq data of SARS-CoV-2 infected colon-(left) and ileum-derived organoids (right). Triangles represent infected cells and colors represent the corrected targeted normalized expression of SARS-CoV-2 determined using the targeted scRNAseq data. B. Proportion of cells infected with SARS-CoV-2 for each cell type and corresponding normalized expression values of SARS-CoV-2 in each individual cell type in colon (left) and ileum (right) organoids. Data are color coded for mock, 12 and 24 hpi. C. Dot plots of known entry determinants across cell types. The dot size represents the percentage of cells expressing the gene; the color represents the average expression across the cell type. Data are from mock-infected colon (left) and ileum organoids (right).
    Figure Legend Snippet: SARS-CoV-2 cell tropism in human colon- and ileum-derived organoids. A. UMAP visualization of scRNAseq data of SARS-CoV-2 infected colon-(left) and ileum-derived organoids (right). Triangles represent infected cells and colors represent the corrected targeted normalized expression of SARS-CoV-2 determined using the targeted scRNAseq data. B. Proportion of cells infected with SARS-CoV-2 for each cell type and corresponding normalized expression values of SARS-CoV-2 in each individual cell type in colon (left) and ileum (right) organoids. Data are color coded for mock, 12 and 24 hpi. C. Dot plots of known entry determinants across cell types. The dot size represents the percentage of cells expressing the gene; the color represents the average expression across the cell type. Data are from mock-infected colon (left) and ileum organoids (right).

    Techniques Used: Derivative Assay, Infection, Expressing

    Schematic of SARS-CoV-2 infection of human intestinal epithelial cells. SARS-CoV-2 infects a subpopulation of enterocytes. Upon infection, enterocytes mount a pro-inflammatory response characterized by the upregulation of NFκB and TNF. Bystander cells respond to secreted IFN and upregulate the expression of ISGs. SARS-CoV-2 infection induces the downregulation of ACE2 expression and interferes with IFN-mediated signalling in infected cells.
    Figure Legend Snippet: Schematic of SARS-CoV-2 infection of human intestinal epithelial cells. SARS-CoV-2 infects a subpopulation of enterocytes. Upon infection, enterocytes mount a pro-inflammatory response characterized by the upregulation of NFκB and TNF. Bystander cells respond to secreted IFN and upregulate the expression of ISGs. SARS-CoV-2 infection induces the downregulation of ACE2 expression and interferes with IFN-mediated signalling in infected cells.

    Techniques Used: Infection, Expressing

    Differential signaling activity of infected vs. bystander cells upon SARS-CoV-2 infection. A-B. Heatmaps of pathway signaling activity inferred by PROGENy for A. colon and B. ileum organoids. C-D. Diffusion maps embeddings showing mock, bystander and infected cells (big panels) and activities for selected transcription factors STAT1, IRF1 and JUN (small panels) for C. colon and D. ileum organoids. E-F. Heatmaps of transcription factor activities along bifurcating trajectories of the corresponding diffusion maps for E. colon and F. ileum organoids. The dimensions DC1 and DC2 represent the first two eigenvectors of the Markovian transition matrix and were calculated separately for either colon or ileum organoids.
    Figure Legend Snippet: Differential signaling activity of infected vs. bystander cells upon SARS-CoV-2 infection. A-B. Heatmaps of pathway signaling activity inferred by PROGENy for A. colon and B. ileum organoids. C-D. Diffusion maps embeddings showing mock, bystander and infected cells (big panels) and activities for selected transcription factors STAT1, IRF1 and JUN (small panels) for C. colon and D. ileum organoids. E-F. Heatmaps of transcription factor activities along bifurcating trajectories of the corresponding diffusion maps for E. colon and F. ileum organoids. The dimensions DC1 and DC2 represent the first two eigenvectors of the Markovian transition matrix and were calculated separately for either colon or ileum organoids.

    Techniques Used: Activity Assay, Infection, Diffusion-based Assay

    Downregulation of ACE2 upon SARS-CoV-2 infection. A. Volcano plots of genes that are differentially expressed in infected cells relative to mock-infected cells at 12 hpi and 24 hpi in ileum organoids. The statistical significance (−log10 of the adjusted p-value) is shown as a function of the log2 fold change. B. Dot plots displaying the expression changes of known SARS-CoV-2 entry determinants for both infected and bystander cells during the course of infection (mock, 12 and 24 hpi) in colon (left) and ileum organoids (right). The dot size represents the percentage of cells expressing the gene; the color represents the average expression across the cell type excluding zeros. C. Correlation of gene expression values with the amount of SARS-CoV-2 genome (y-axis) vs the maximal log2 fold change (x-axis) across conditions. This plot is generated by comparing both 12 and 24 hpi to mock. D. Correlation of SARS-CoV-2 expression with ACE2 expression across all cell types at 24 hpi. E. Expression values of ACE2 in each cell type for mock infected and SARS-CoV-2 infected cells in ileum organoids at 12 hpi and 24 hpi. F. Multiplex in situ RNA hybridization of ACE2 and SARS-CoV-2 of mock-infected and infected 2D ileum organoids at 12 hpi and 24 hpi. White arrows point at SARS-CoV-2-infected cells. Representative image is shown. G. Correlation of the relative expression SARS-CoV-2 and ACE2 for infected cells from the multiplex in situ RNA hybridization shown in F. Each dot represents a cell.
    Figure Legend Snippet: Downregulation of ACE2 upon SARS-CoV-2 infection. A. Volcano plots of genes that are differentially expressed in infected cells relative to mock-infected cells at 12 hpi and 24 hpi in ileum organoids. The statistical significance (−log10 of the adjusted p-value) is shown as a function of the log2 fold change. B. Dot plots displaying the expression changes of known SARS-CoV-2 entry determinants for both infected and bystander cells during the course of infection (mock, 12 and 24 hpi) in colon (left) and ileum organoids (right). The dot size represents the percentage of cells expressing the gene; the color represents the average expression across the cell type excluding zeros. C. Correlation of gene expression values with the amount of SARS-CoV-2 genome (y-axis) vs the maximal log2 fold change (x-axis) across conditions. This plot is generated by comparing both 12 and 24 hpi to mock. D. Correlation of SARS-CoV-2 expression with ACE2 expression across all cell types at 24 hpi. E. Expression values of ACE2 in each cell type for mock infected and SARS-CoV-2 infected cells in ileum organoids at 12 hpi and 24 hpi. F. Multiplex in situ RNA hybridization of ACE2 and SARS-CoV-2 of mock-infected and infected 2D ileum organoids at 12 hpi and 24 hpi. White arrows point at SARS-CoV-2-infected cells. Representative image is shown. G. Correlation of the relative expression SARS-CoV-2 and ACE2 for infected cells from the multiplex in situ RNA hybridization shown in F. Each dot represents a cell.

    Techniques Used: Infection, Expressing, Generated, Multiplex Assay, In Situ, Hybridization

    Intrinsic innate immune response generated by immature enterocytes 2 upon SARS-CoV-2 infection. (A-C) Volcano plots displaying the genes that are differentially expressed in immature enterocytes 2 upon SARS-CoV-2 infection of colon organoids. A. infected vs. mock infected cells, B. bystander vs. mock infected cells and C. infected vs. bystander cells. The statistical significance (−log10 adjusted p-value) is shown as a function of the log2 fold change. D. Dot plot of the top 42 most differentially expressed genes upon SARS-CoV-2 infection in mock, infected and bystander cells at 12 and 24 hpi. The dot size represents the percentage of cells expressing the gene; the color represents the average expression across the cell type. E. Same as in D but for the top 30 most differentially expressed ISGs. F-G. Same as A-E but for ileum organoids.
    Figure Legend Snippet: Intrinsic innate immune response generated by immature enterocytes 2 upon SARS-CoV-2 infection. (A-C) Volcano plots displaying the genes that are differentially expressed in immature enterocytes 2 upon SARS-CoV-2 infection of colon organoids. A. infected vs. mock infected cells, B. bystander vs. mock infected cells and C. infected vs. bystander cells. The statistical significance (−log10 adjusted p-value) is shown as a function of the log2 fold change. D. Dot plot of the top 42 most differentially expressed genes upon SARS-CoV-2 infection in mock, infected and bystander cells at 12 and 24 hpi. The dot size represents the percentage of cells expressing the gene; the color represents the average expression across the cell type. E. Same as in D but for the top 30 most differentially expressed ISGs. F-G. Same as A-E but for ileum organoids.

    Techniques Used: Generated, Infection, Expressing

    Single cell sequencing of SARS-CoV-2 infected colon- and ileum-derived human organoids. A. Schematic representation of the experimental workflow. B. Uniform manifold approximation and projection (UMAP) embedding of single-cell RNA-Seq data from mock and SARS-CoV-2 infected colon-derived (left panels) and ileum-derived (right panels) organoids colored according to the cell type. Small insets represent the UMAP for mock and infected organoids at 12 and 24 hpi. C. Dot plot of the top marker genes for each cell type for (left) colon- and (right) ileum-derived organoids. The dot size represents the percentage of cells expressing the gene; the color represents the average expression across the cell type. D. Bar plot displaying the proportion of each cell type in mock and infected organoids (12 and 24 hpi).
    Figure Legend Snippet: Single cell sequencing of SARS-CoV-2 infected colon- and ileum-derived human organoids. A. Schematic representation of the experimental workflow. B. Uniform manifold approximation and projection (UMAP) embedding of single-cell RNA-Seq data from mock and SARS-CoV-2 infected colon-derived (left panels) and ileum-derived (right panels) organoids colored according to the cell type. Small insets represent the UMAP for mock and infected organoids at 12 and 24 hpi. C. Dot plot of the top marker genes for each cell type for (left) colon- and (right) ileum-derived organoids. The dot size represents the percentage of cells expressing the gene; the color represents the average expression across the cell type. D. Bar plot displaying the proportion of each cell type in mock and infected organoids (12 and 24 hpi).

    Techniques Used: Sequencing, Infection, Derivative Assay, RNA Sequencing Assay, Marker, Expressing

    SARS-CoV-2 infection impairs interferon-mediated signaling. A. Human ileum-derived organoids were seeded in 2D on iBIDi chamber slides. 12 and 24 hpi cells were fixed and the amount of SARS-CoV-2 infected cells (red) and the induction of ISG15 (white) was analyzed by single molecule RNA FISH. Nuclei were visualized with DAPI (blue). Representative image is shown. B. Quantification of the mean fluorescence intensity of samples in A. Each dot represents a cell. C. Schematic depicting the infection and interferon addition to evaluate ISG15 induction following SARS-CoV-2 and astrovirus infection. D. T84 IRF3 knock-out cells were infected with SARS-CoV-2 or human astrovirus 1 at an MOI=3. 24 hpi, cells were incubated in the presence or absence of 2000 IU/mL of IFNβ1. 12 h post-treatment RNA was harvested and the induction of ISG15 was analyzed by q-RT-PCR. Three biological replicates were performed. Error bar indicates standard deviation. Statistics were determined by unpaired t-test. E. T84 IRF3 knock-out cells were infected with SARS-CoV-2 or human astrovirus 1 at an MOI=3. 24 hpi, cells were fixed and stained for either the SARS-CoV-2 N protein or for human astrovirus 1 (HAstV1) capsid protein. Experiments were performed in triplicate. Representative image is shown. F. Schematic depicting the T84 MX-1 mcherry expressing cells and their response to IFN or SARS-CoV-2 infection. G. T84 MX-1 mcherry were infected with SARS-CoV-2 at an MOI=3 or MOI=1. 24 hpi cells were incubated in the presence or absence of 2000 IU/mL of IFNβ1. 12 h post-treatment cells were fixed and stained for SARS-CoV-2 N protein. The number of MX-1 positive, SARS-CoV-2 positive and double positive cells was quantified. Three biological replicates were performed. Error bar indicates standard deviation.
    Figure Legend Snippet: SARS-CoV-2 infection impairs interferon-mediated signaling. A. Human ileum-derived organoids were seeded in 2D on iBIDi chamber slides. 12 and 24 hpi cells were fixed and the amount of SARS-CoV-2 infected cells (red) and the induction of ISG15 (white) was analyzed by single molecule RNA FISH. Nuclei were visualized with DAPI (blue). Representative image is shown. B. Quantification of the mean fluorescence intensity of samples in A. Each dot represents a cell. C. Schematic depicting the infection and interferon addition to evaluate ISG15 induction following SARS-CoV-2 and astrovirus infection. D. T84 IRF3 knock-out cells were infected with SARS-CoV-2 or human astrovirus 1 at an MOI=3. 24 hpi, cells were incubated in the presence or absence of 2000 IU/mL of IFNβ1. 12 h post-treatment RNA was harvested and the induction of ISG15 was analyzed by q-RT-PCR. Three biological replicates were performed. Error bar indicates standard deviation. Statistics were determined by unpaired t-test. E. T84 IRF3 knock-out cells were infected with SARS-CoV-2 or human astrovirus 1 at an MOI=3. 24 hpi, cells were fixed and stained for either the SARS-CoV-2 N protein or for human astrovirus 1 (HAstV1) capsid protein. Experiments were performed in triplicate. Representative image is shown. F. Schematic depicting the T84 MX-1 mcherry expressing cells and their response to IFN or SARS-CoV-2 infection. G. T84 MX-1 mcherry were infected with SARS-CoV-2 at an MOI=3 or MOI=1. 24 hpi cells were incubated in the presence or absence of 2000 IU/mL of IFNβ1. 12 h post-treatment cells were fixed and stained for SARS-CoV-2 N protein. The number of MX-1 positive, SARS-CoV-2 positive and double positive cells was quantified. Three biological replicates were performed. Error bar indicates standard deviation.

    Techniques Used: Infection, Derivative Assay, Fluorescence In Situ Hybridization, Fluorescence, Knock-Out, Incubation, Reverse Transcription Polymerase Chain Reaction, Standard Deviation, Staining, Expressing

    3) Product Images from "“Acute Respiratory Distress and Cytokine Storm in Aged, SARS-CoV-2 Infected African Green Monkeys, but not in Rhesus Macaques”"

    Article Title: “Acute Respiratory Distress and Cytokine Storm in Aged, SARS-CoV-2 Infected African Green Monkeys, but not in Rhesus Macaques”

    Journal: bioRxiv

    doi: 10.1101/2020.06.18.157933

    Histopathology and fluorescent immunohistochemistry in AGM1. ( A ) The right lower lung lobe is filled with fibrin and edema with areas of hemorrhage and necrosis (arrows); Bar = 5 mm. ( B ) Alveoli are variably lined by hyaline membranes (arrows) and type II pneumocytes (arrowheads); Bar = 100 um. ( C ) Rare multinucleated syncytia (arrow) are scattered throughout the affected lungs; Bar = 50 um. ( D ) Fluorescent immunohistochemistry for COV-2 nucleoprotein (green, arrows) and ACE2 (red) identified low numbers of CoV-2 positive cells within the affected lung lobes; Bar = 100 um. White: DAPI/nuclei; Green: CoV-2; Red: ACE2 Blue: Empty.
    Figure Legend Snippet: Histopathology and fluorescent immunohistochemistry in AGM1. ( A ) The right lower lung lobe is filled with fibrin and edema with areas of hemorrhage and necrosis (arrows); Bar = 5 mm. ( B ) Alveoli are variably lined by hyaline membranes (arrows) and type II pneumocytes (arrowheads); Bar = 100 um. ( C ) Rare multinucleated syncytia (arrow) are scattered throughout the affected lungs; Bar = 50 um. ( D ) Fluorescent immunohistochemistry for COV-2 nucleoprotein (green, arrows) and ACE2 (red) identified low numbers of CoV-2 positive cells within the affected lung lobes; Bar = 100 um. White: DAPI/nuclei; Green: CoV-2; Red: ACE2 Blue: Empty.

    Techniques Used: Histopathology, Immunohistochemistry

    Related Articles

    Clone Assay:

    Article Title: Identification of Immunohistochemical Reagents for In Situ Protein Expression Analysis of Coronavirus-associated Changes in Human Tissues
    Article Snippet: .. Our study demonstrates that rigid specificity testing is mandatory for the evaluation of mAbs to SARS-CoV2 and that clones 001 to nucleoprotein and 1A9 to S2 subunit spike protein are useful for the in situ detection of SARS-CoV2. ..

    Transfection:

    Article Title: Identification of Immunohistochemical Reagents for In Situ Protein Expression Analysis of Coronavirus-associated Changes in Human Tissues
    Article Snippet: .. Antibodies were screened on HEK293 cells transfected with viral nucleoprotein, S1 subunit and S2 subunit of spike protein and on untransfected cells, as well as a panel of normal tissue. .. Lung tissue with presence of SARS-CoV2 confirmed by in situ hybridization (ISH) was also used.

    Article Title: Inhibition of Proprotein Convertases Abrogates Processing of the Middle Eastern Respiratory Syndrome Coronavirus Spike Protein in Infected Cells but Does Not Reduce Viral Infectivity
    Article Snippet: .. Western blot analysis of S protein transfected 293T cells and MERS-CoV–infected Vero B4 cells with an antibody specific to the S2 subunit of MERS-S revealed 2 prominent S protein bands with molecular weights of 170 kDa and 90 kDa (Figure ), in keeping with our previous results [ ]. ..

    In Situ:

    Article Title: Identification of Immunohistochemical Reagents for In Situ Protein Expression Analysis of Coronavirus-associated Changes in Human Tissues
    Article Snippet: .. Our study demonstrates that rigid specificity testing is mandatory for the evaluation of mAbs to SARS-CoV2 and that clones 001 to nucleoprotein and 1A9 to S2 subunit spike protein are useful for the in situ detection of SARS-CoV2. ..

    other:

    Article Title: Identification of Immunohistochemical Reagents for In Situ Protein Expression Analysis of Coronavirus-associated Changes in Human Tissues
    Article Snippet: Only 2 mAbs, clone 001 to the nucleoprotein and clone 1A9 to the S2 subunit spike protein displayed specific immunoreactivity.

    Expressing:

    Article Title: Inhibition of Proprotein Convertases Abrogates Processing of the Middle Eastern Respiratory Syndrome Coronavirus Spike Protein in Infected Cells but Does Not Reduce Viral Infectivity
    Article Snippet: .. MERS-S expression was detected using a monoclonal antibody directed against the V5 tag (Invitrogen) or a polyclonal antibody directed against the S2 subunit of the MERS-S protein (Sino Biological). .. For detection of MERS-S protein in infected cells, Caco-2 and Vero B4 cells were infected with MERS-CoV (human betacoronavirus 2c EMC/2012) at a multiplicity of infection (MOI) of 0.01 and 5, respectively.

    Article Title: Inhibition of Proprotein Convertases Abrogates Processing of the Middle Eastern Respiratory Syndrome Coronavirus Spike Protein in Infected Cells but Does Not Reduce Viral Infectivity
    Article Snippet: .. S protein expression in lysates was detected by Western blot, using a polyclonal antibody directed against the S2 subunit of MERS-S (Sino Biological). .. In parallel, for quantification of viral RNA, 50 µL of the cell supernatant was dissolved in RAV1 buffer (Macherey-Nagel) for RNA extraction, followed by quantitative reverse-transcription PCR analysis, using the upE assay as previously described [ ].

    Western Blot:

    Article Title: Inhibition of Proprotein Convertases Abrogates Processing of the Middle Eastern Respiratory Syndrome Coronavirus Spike Protein in Infected Cells but Does Not Reduce Viral Infectivity
    Article Snippet: .. S protein expression in lysates was detected by Western blot, using a polyclonal antibody directed against the S2 subunit of MERS-S (Sino Biological). .. In parallel, for quantification of viral RNA, 50 µL of the cell supernatant was dissolved in RAV1 buffer (Macherey-Nagel) for RNA extraction, followed by quantitative reverse-transcription PCR analysis, using the upE assay as previously described [ ].

    Article Title: Inhibition of Proprotein Convertases Abrogates Processing of the Middle Eastern Respiratory Syndrome Coronavirus Spike Protein in Infected Cells but Does Not Reduce Viral Infectivity
    Article Snippet: .. Western blot analysis of S protein transfected 293T cells and MERS-CoV–infected Vero B4 cells with an antibody specific to the S2 subunit of MERS-S revealed 2 prominent S protein bands with molecular weights of 170 kDa and 90 kDa (Figure ), in keeping with our previous results [ ]. ..

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  • 93
    Sino Biological mouse monoclonal antibody against sars cov np
    <t>SARS-CoV-2</t> cell tropism in human colon- and ileum-derived organoids. A. UMAP visualization of scRNAseq data of SARS-CoV-2 infected colon-(left) and ileum-derived organoids (right). Triangles represent infected cells and colors represent the corrected targeted normalized expression of SARS-CoV-2 determined using the targeted scRNAseq data. B. Proportion of cells infected with SARS-CoV-2 for each cell type and corresponding normalized expression values of SARS-CoV-2 in each individual cell type in colon (left) and ileum (right) organoids. Data are color coded for mock, 12 and 24 hpi. C. Dot plots of known entry determinants across cell types. The dot size represents the percentage of cells expressing the gene; the color represents the average expression across the cell type. Data are from mock-infected colon (left) and ileum organoids (right).
    Mouse Monoclonal Antibody Against Sars Cov Np, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse monoclonal antibody against sars cov np/product/Sino Biological
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse monoclonal antibody against sars cov np - by Bioz Stars, 2021-02
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    Sino Biological antibodies
    <t>SARS-CoV-2</t> cell tropism in human colon- and ileum-derived organoids. A. UMAP visualization of scRNAseq data of SARS-CoV-2 infected colon-(left) and ileum-derived organoids (right). Triangles represent infected cells and colors represent the corrected targeted normalized expression of SARS-CoV-2 determined using the targeted scRNAseq data. B. Proportion of cells infected with SARS-CoV-2 for each cell type and corresponding normalized expression values of SARS-CoV-2 in each individual cell type in colon (left) and ileum (right) organoids. Data are color coded for mock, 12 and 24 hpi. C. Dot plots of known entry determinants across cell types. The dot size represents the percentage of cells expressing the gene; the color represents the average expression across the cell type. Data are from mock-infected colon (left) and ileum organoids (right).
    Antibodies, supplied by Sino Biological, used in various techniques. Bioz Stars score: 92/100, based on 0 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibodies/product/Sino Biological
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antibodies - by Bioz Stars, 2021-02
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    Sino Biological mouse monoclonal gb specific igg1 antibody
    <t>SARS-CoV-2</t> cell tropism in human colon- and ileum-derived organoids. A. UMAP visualization of scRNAseq data of SARS-CoV-2 infected colon-(left) and ileum-derived organoids (right). Triangles represent infected cells and colors represent the corrected targeted normalized expression of SARS-CoV-2 determined using the targeted scRNAseq data. B. Proportion of cells infected with SARS-CoV-2 for each cell type and corresponding normalized expression values of SARS-CoV-2 in each individual cell type in colon (left) and ileum (right) organoids. Data are color coded for mock, 12 and 24 hpi. C. Dot plots of known entry determinants across cell types. The dot size represents the percentage of cells expressing the gene; the color represents the average expression across the cell type. Data are from mock-infected colon (left) and ileum organoids (right).
    Mouse Monoclonal Gb Specific Igg1 Antibody, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse monoclonal gb specific igg1 antibody/product/Sino Biological
    Average 93 stars, based on 1 article reviews
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    Sino Biological anti mouse igg
    <t>SARS-CoV-2</t> cell tropism in human colon- and ileum-derived organoids. A. UMAP visualization of scRNAseq data of SARS-CoV-2 infected colon-(left) and ileum-derived organoids (right). Triangles represent infected cells and colors represent the corrected targeted normalized expression of SARS-CoV-2 determined using the targeted scRNAseq data. B. Proportion of cells infected with SARS-CoV-2 for each cell type and corresponding normalized expression values of SARS-CoV-2 in each individual cell type in colon (left) and ileum (right) organoids. Data are color coded for mock, 12 and 24 hpi. C. Dot plots of known entry determinants across cell types. The dot size represents the percentage of cells expressing the gene; the color represents the average expression across the cell type. Data are from mock-infected colon (left) and ileum organoids (right).
    Anti Mouse Igg, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 0 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti mouse igg/product/Sino Biological
    Average 93 stars, based on 1 article reviews
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    SARS-CoV-2 cell tropism in human colon- and ileum-derived organoids. A. UMAP visualization of scRNAseq data of SARS-CoV-2 infected colon-(left) and ileum-derived organoids (right). Triangles represent infected cells and colors represent the corrected targeted normalized expression of SARS-CoV-2 determined using the targeted scRNAseq data. B. Proportion of cells infected with SARS-CoV-2 for each cell type and corresponding normalized expression values of SARS-CoV-2 in each individual cell type in colon (left) and ileum (right) organoids. Data are color coded for mock, 12 and 24 hpi. C. Dot plots of known entry determinants across cell types. The dot size represents the percentage of cells expressing the gene; the color represents the average expression across the cell type. Data are from mock-infected colon (left) and ileum organoids (right).

    Journal: bioRxiv

    Article Title: Single-cell analyses reveal SARS-CoV-2 interference with intrinsic immune response in the human gut

    doi: 10.1101/2020.10.21.348854

    Figure Lengend Snippet: SARS-CoV-2 cell tropism in human colon- and ileum-derived organoids. A. UMAP visualization of scRNAseq data of SARS-CoV-2 infected colon-(left) and ileum-derived organoids (right). Triangles represent infected cells and colors represent the corrected targeted normalized expression of SARS-CoV-2 determined using the targeted scRNAseq data. B. Proportion of cells infected with SARS-CoV-2 for each cell type and corresponding normalized expression values of SARS-CoV-2 in each individual cell type in colon (left) and ileum (right) organoids. Data are color coded for mock, 12 and 24 hpi. C. Dot plots of known entry determinants across cell types. The dot size represents the percentage of cells expressing the gene; the color represents the average expression across the cell type. Data are from mock-infected colon (left) and ileum organoids (right).

    Article Snippet: Mouse monoclonal antibody against SARS-CoV NP (Sino biologicals MM05) and mouse monoclonal against J2 (scions) were diluted in phosphate-buffered saline (PBS) at 1/1000 dilution and incubated for 1h at RT.

    Techniques: Derivative Assay, Infection, Expressing

    Schematic of SARS-CoV-2 infection of human intestinal epithelial cells. SARS-CoV-2 infects a subpopulation of enterocytes. Upon infection, enterocytes mount a pro-inflammatory response characterized by the upregulation of NFκB and TNF. Bystander cells respond to secreted IFN and upregulate the expression of ISGs. SARS-CoV-2 infection induces the downregulation of ACE2 expression and interferes with IFN-mediated signalling in infected cells.

    Journal: bioRxiv

    Article Title: Single-cell analyses reveal SARS-CoV-2 interference with intrinsic immune response in the human gut

    doi: 10.1101/2020.10.21.348854

    Figure Lengend Snippet: Schematic of SARS-CoV-2 infection of human intestinal epithelial cells. SARS-CoV-2 infects a subpopulation of enterocytes. Upon infection, enterocytes mount a pro-inflammatory response characterized by the upregulation of NFκB and TNF. Bystander cells respond to secreted IFN and upregulate the expression of ISGs. SARS-CoV-2 infection induces the downregulation of ACE2 expression and interferes with IFN-mediated signalling in infected cells.

    Article Snippet: Mouse monoclonal antibody against SARS-CoV NP (Sino biologicals MM05) and mouse monoclonal against J2 (scions) were diluted in phosphate-buffered saline (PBS) at 1/1000 dilution and incubated for 1h at RT.

    Techniques: Infection, Expressing

    Differential signaling activity of infected vs. bystander cells upon SARS-CoV-2 infection. A-B. Heatmaps of pathway signaling activity inferred by PROGENy for A. colon and B. ileum organoids. C-D. Diffusion maps embeddings showing mock, bystander and infected cells (big panels) and activities for selected transcription factors STAT1, IRF1 and JUN (small panels) for C. colon and D. ileum organoids. E-F. Heatmaps of transcription factor activities along bifurcating trajectories of the corresponding diffusion maps for E. colon and F. ileum organoids. The dimensions DC1 and DC2 represent the first two eigenvectors of the Markovian transition matrix and were calculated separately for either colon or ileum organoids.

    Journal: bioRxiv

    Article Title: Single-cell analyses reveal SARS-CoV-2 interference with intrinsic immune response in the human gut

    doi: 10.1101/2020.10.21.348854

    Figure Lengend Snippet: Differential signaling activity of infected vs. bystander cells upon SARS-CoV-2 infection. A-B. Heatmaps of pathway signaling activity inferred by PROGENy for A. colon and B. ileum organoids. C-D. Diffusion maps embeddings showing mock, bystander and infected cells (big panels) and activities for selected transcription factors STAT1, IRF1 and JUN (small panels) for C. colon and D. ileum organoids. E-F. Heatmaps of transcription factor activities along bifurcating trajectories of the corresponding diffusion maps for E. colon and F. ileum organoids. The dimensions DC1 and DC2 represent the first two eigenvectors of the Markovian transition matrix and were calculated separately for either colon or ileum organoids.

    Article Snippet: Mouse monoclonal antibody against SARS-CoV NP (Sino biologicals MM05) and mouse monoclonal against J2 (scions) were diluted in phosphate-buffered saline (PBS) at 1/1000 dilution and incubated for 1h at RT.

    Techniques: Activity Assay, Infection, Diffusion-based Assay

    Downregulation of ACE2 upon SARS-CoV-2 infection. A. Volcano plots of genes that are differentially expressed in infected cells relative to mock-infected cells at 12 hpi and 24 hpi in ileum organoids. The statistical significance (−log10 of the adjusted p-value) is shown as a function of the log2 fold change. B. Dot plots displaying the expression changes of known SARS-CoV-2 entry determinants for both infected and bystander cells during the course of infection (mock, 12 and 24 hpi) in colon (left) and ileum organoids (right). The dot size represents the percentage of cells expressing the gene; the color represents the average expression across the cell type excluding zeros. C. Correlation of gene expression values with the amount of SARS-CoV-2 genome (y-axis) vs the maximal log2 fold change (x-axis) across conditions. This plot is generated by comparing both 12 and 24 hpi to mock. D. Correlation of SARS-CoV-2 expression with ACE2 expression across all cell types at 24 hpi. E. Expression values of ACE2 in each cell type for mock infected and SARS-CoV-2 infected cells in ileum organoids at 12 hpi and 24 hpi. F. Multiplex in situ RNA hybridization of ACE2 and SARS-CoV-2 of mock-infected and infected 2D ileum organoids at 12 hpi and 24 hpi. White arrows point at SARS-CoV-2-infected cells. Representative image is shown. G. Correlation of the relative expression SARS-CoV-2 and ACE2 for infected cells from the multiplex in situ RNA hybridization shown in F. Each dot represents a cell.

    Journal: bioRxiv

    Article Title: Single-cell analyses reveal SARS-CoV-2 interference with intrinsic immune response in the human gut

    doi: 10.1101/2020.10.21.348854

    Figure Lengend Snippet: Downregulation of ACE2 upon SARS-CoV-2 infection. A. Volcano plots of genes that are differentially expressed in infected cells relative to mock-infected cells at 12 hpi and 24 hpi in ileum organoids. The statistical significance (−log10 of the adjusted p-value) is shown as a function of the log2 fold change. B. Dot plots displaying the expression changes of known SARS-CoV-2 entry determinants for both infected and bystander cells during the course of infection (mock, 12 and 24 hpi) in colon (left) and ileum organoids (right). The dot size represents the percentage of cells expressing the gene; the color represents the average expression across the cell type excluding zeros. C. Correlation of gene expression values with the amount of SARS-CoV-2 genome (y-axis) vs the maximal log2 fold change (x-axis) across conditions. This plot is generated by comparing both 12 and 24 hpi to mock. D. Correlation of SARS-CoV-2 expression with ACE2 expression across all cell types at 24 hpi. E. Expression values of ACE2 in each cell type for mock infected and SARS-CoV-2 infected cells in ileum organoids at 12 hpi and 24 hpi. F. Multiplex in situ RNA hybridization of ACE2 and SARS-CoV-2 of mock-infected and infected 2D ileum organoids at 12 hpi and 24 hpi. White arrows point at SARS-CoV-2-infected cells. Representative image is shown. G. Correlation of the relative expression SARS-CoV-2 and ACE2 for infected cells from the multiplex in situ RNA hybridization shown in F. Each dot represents a cell.

    Article Snippet: Mouse monoclonal antibody against SARS-CoV NP (Sino biologicals MM05) and mouse monoclonal against J2 (scions) were diluted in phosphate-buffered saline (PBS) at 1/1000 dilution and incubated for 1h at RT.

    Techniques: Infection, Expressing, Generated, Multiplex Assay, In Situ, Hybridization

    Intrinsic innate immune response generated by immature enterocytes 2 upon SARS-CoV-2 infection. (A-C) Volcano plots displaying the genes that are differentially expressed in immature enterocytes 2 upon SARS-CoV-2 infection of colon organoids. A. infected vs. mock infected cells, B. bystander vs. mock infected cells and C. infected vs. bystander cells. The statistical significance (−log10 adjusted p-value) is shown as a function of the log2 fold change. D. Dot plot of the top 42 most differentially expressed genes upon SARS-CoV-2 infection in mock, infected and bystander cells at 12 and 24 hpi. The dot size represents the percentage of cells expressing the gene; the color represents the average expression across the cell type. E. Same as in D but for the top 30 most differentially expressed ISGs. F-G. Same as A-E but for ileum organoids.

    Journal: bioRxiv

    Article Title: Single-cell analyses reveal SARS-CoV-2 interference with intrinsic immune response in the human gut

    doi: 10.1101/2020.10.21.348854

    Figure Lengend Snippet: Intrinsic innate immune response generated by immature enterocytes 2 upon SARS-CoV-2 infection. (A-C) Volcano plots displaying the genes that are differentially expressed in immature enterocytes 2 upon SARS-CoV-2 infection of colon organoids. A. infected vs. mock infected cells, B. bystander vs. mock infected cells and C. infected vs. bystander cells. The statistical significance (−log10 adjusted p-value) is shown as a function of the log2 fold change. D. Dot plot of the top 42 most differentially expressed genes upon SARS-CoV-2 infection in mock, infected and bystander cells at 12 and 24 hpi. The dot size represents the percentage of cells expressing the gene; the color represents the average expression across the cell type. E. Same as in D but for the top 30 most differentially expressed ISGs. F-G. Same as A-E but for ileum organoids.

    Article Snippet: Mouse monoclonal antibody against SARS-CoV NP (Sino biologicals MM05) and mouse monoclonal against J2 (scions) were diluted in phosphate-buffered saline (PBS) at 1/1000 dilution and incubated for 1h at RT.

    Techniques: Generated, Infection, Expressing

    Single cell sequencing of SARS-CoV-2 infected colon- and ileum-derived human organoids. A. Schematic representation of the experimental workflow. B. Uniform manifold approximation and projection (UMAP) embedding of single-cell RNA-Seq data from mock and SARS-CoV-2 infected colon-derived (left panels) and ileum-derived (right panels) organoids colored according to the cell type. Small insets represent the UMAP for mock and infected organoids at 12 and 24 hpi. C. Dot plot of the top marker genes for each cell type for (left) colon- and (right) ileum-derived organoids. The dot size represents the percentage of cells expressing the gene; the color represents the average expression across the cell type. D. Bar plot displaying the proportion of each cell type in mock and infected organoids (12 and 24 hpi).

    Journal: bioRxiv

    Article Title: Single-cell analyses reveal SARS-CoV-2 interference with intrinsic immune response in the human gut

    doi: 10.1101/2020.10.21.348854

    Figure Lengend Snippet: Single cell sequencing of SARS-CoV-2 infected colon- and ileum-derived human organoids. A. Schematic representation of the experimental workflow. B. Uniform manifold approximation and projection (UMAP) embedding of single-cell RNA-Seq data from mock and SARS-CoV-2 infected colon-derived (left panels) and ileum-derived (right panels) organoids colored according to the cell type. Small insets represent the UMAP for mock and infected organoids at 12 and 24 hpi. C. Dot plot of the top marker genes for each cell type for (left) colon- and (right) ileum-derived organoids. The dot size represents the percentage of cells expressing the gene; the color represents the average expression across the cell type. D. Bar plot displaying the proportion of each cell type in mock and infected organoids (12 and 24 hpi).

    Article Snippet: Mouse monoclonal antibody against SARS-CoV NP (Sino biologicals MM05) and mouse monoclonal against J2 (scions) were diluted in phosphate-buffered saline (PBS) at 1/1000 dilution and incubated for 1h at RT.

    Techniques: Sequencing, Infection, Derivative Assay, RNA Sequencing Assay, Marker, Expressing

    SARS-CoV-2 infection impairs interferon-mediated signaling. A. Human ileum-derived organoids were seeded in 2D on iBIDi chamber slides. 12 and 24 hpi cells were fixed and the amount of SARS-CoV-2 infected cells (red) and the induction of ISG15 (white) was analyzed by single molecule RNA FISH. Nuclei were visualized with DAPI (blue). Representative image is shown. B. Quantification of the mean fluorescence intensity of samples in A. Each dot represents a cell. C. Schematic depicting the infection and interferon addition to evaluate ISG15 induction following SARS-CoV-2 and astrovirus infection. D. T84 IRF3 knock-out cells were infected with SARS-CoV-2 or human astrovirus 1 at an MOI=3. 24 hpi, cells were incubated in the presence or absence of 2000 IU/mL of IFNβ1. 12 h post-treatment RNA was harvested and the induction of ISG15 was analyzed by q-RT-PCR. Three biological replicates were performed. Error bar indicates standard deviation. Statistics were determined by unpaired t-test. E. T84 IRF3 knock-out cells were infected with SARS-CoV-2 or human astrovirus 1 at an MOI=3. 24 hpi, cells were fixed and stained for either the SARS-CoV-2 N protein or for human astrovirus 1 (HAstV1) capsid protein. Experiments were performed in triplicate. Representative image is shown. F. Schematic depicting the T84 MX-1 mcherry expressing cells and their response to IFN or SARS-CoV-2 infection. G. T84 MX-1 mcherry were infected with SARS-CoV-2 at an MOI=3 or MOI=1. 24 hpi cells were incubated in the presence or absence of 2000 IU/mL of IFNβ1. 12 h post-treatment cells were fixed and stained for SARS-CoV-2 N protein. The number of MX-1 positive, SARS-CoV-2 positive and double positive cells was quantified. Three biological replicates were performed. Error bar indicates standard deviation.

    Journal: bioRxiv

    Article Title: Single-cell analyses reveal SARS-CoV-2 interference with intrinsic immune response in the human gut

    doi: 10.1101/2020.10.21.348854

    Figure Lengend Snippet: SARS-CoV-2 infection impairs interferon-mediated signaling. A. Human ileum-derived organoids were seeded in 2D on iBIDi chamber slides. 12 and 24 hpi cells were fixed and the amount of SARS-CoV-2 infected cells (red) and the induction of ISG15 (white) was analyzed by single molecule RNA FISH. Nuclei were visualized with DAPI (blue). Representative image is shown. B. Quantification of the mean fluorescence intensity of samples in A. Each dot represents a cell. C. Schematic depicting the infection and interferon addition to evaluate ISG15 induction following SARS-CoV-2 and astrovirus infection. D. T84 IRF3 knock-out cells were infected with SARS-CoV-2 or human astrovirus 1 at an MOI=3. 24 hpi, cells were incubated in the presence or absence of 2000 IU/mL of IFNβ1. 12 h post-treatment RNA was harvested and the induction of ISG15 was analyzed by q-RT-PCR. Three biological replicates were performed. Error bar indicates standard deviation. Statistics were determined by unpaired t-test. E. T84 IRF3 knock-out cells were infected with SARS-CoV-2 or human astrovirus 1 at an MOI=3. 24 hpi, cells were fixed and stained for either the SARS-CoV-2 N protein or for human astrovirus 1 (HAstV1) capsid protein. Experiments were performed in triplicate. Representative image is shown. F. Schematic depicting the T84 MX-1 mcherry expressing cells and their response to IFN or SARS-CoV-2 infection. G. T84 MX-1 mcherry were infected with SARS-CoV-2 at an MOI=3 or MOI=1. 24 hpi cells were incubated in the presence or absence of 2000 IU/mL of IFNβ1. 12 h post-treatment cells were fixed and stained for SARS-CoV-2 N protein. The number of MX-1 positive, SARS-CoV-2 positive and double positive cells was quantified. Three biological replicates were performed. Error bar indicates standard deviation.

    Article Snippet: Mouse monoclonal antibody against SARS-CoV NP (Sino biologicals MM05) and mouse monoclonal against J2 (scions) were diluted in phosphate-buffered saline (PBS) at 1/1000 dilution and incubated for 1h at RT.

    Techniques: Infection, Derivative Assay, Fluorescence In Situ Hybridization, Fluorescence, Knock-Out, Incubation, Reverse Transcription Polymerase Chain Reaction, Standard Deviation, Staining, Expressing