anti h7n9 ha mouse monoclonal antibody  (Sino Biological)


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    Sino Biological anti h7n9 ha mouse monoclonal antibody
    Robust HA-specific IgG antibody titers and hemagglutination-inhibition titers in the sera of the immunized mice. (A) IgG antibodies against <t>H7N9</t> A/Shanghai/1/2013 influenza HA protein. (B) IgG antibodies against H7N9 A/Anhui/1/2013 influenza HA protein.
    Anti H7n9 Ha Mouse Monoclonal Antibody, supplied by Sino Biological, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti h7n9 ha mouse monoclonal antibody/product/Sino Biological
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti h7n9 ha mouse monoclonal antibody - by Bioz Stars, 2021-02
    85/100 stars

    Images

    1) Product Images from "Protective Immunity to H7N9 Influenza viruses elicited by synthetic DNA Vaccine"

    Article Title: Protective Immunity to H7N9 Influenza viruses elicited by synthetic DNA Vaccine

    Journal: Vaccine

    doi: 10.1016/j.vaccine.2014.02.038

    Robust HA-specific IgG antibody titers and hemagglutination-inhibition titers in the sera of the immunized mice. (A) IgG antibodies against H7N9 A/Shanghai/1/2013 influenza HA protein. (B) IgG antibodies against H7N9 A/Anhui/1/2013 influenza HA protein.
    Figure Legend Snippet: Robust HA-specific IgG antibody titers and hemagglutination-inhibition titers in the sera of the immunized mice. (A) IgG antibodies against H7N9 A/Shanghai/1/2013 influenza HA protein. (B) IgG antibodies against H7N9 A/Anhui/1/2013 influenza HA protein.

    Techniques Used: HI Assay, Mouse Assay

    Protection from H7N9 A/Anhui/1/2013 virus challenge in the immunized mice. (A) Experimental schedule of challenge study. The mice (n=10) were immunized with 25 μg of pH7HA twice, three weeks apart. Four weeks after the second immunization, the
    Figure Legend Snippet: Protection from H7N9 A/Anhui/1/2013 virus challenge in the immunized mice. (A) Experimental schedule of challenge study. The mice (n=10) were immunized with 25 μg of pH7HA twice, three weeks apart. Four weeks after the second immunization, the

    Techniques Used: Mouse Assay

    The H7N9 HA DNA vaccine design and its expression (A) Phylogenetic tree based on neighbor-joining evaluation of H7HA alignment. Asterisks indicate location of consensus sequence. The strains used to generate the consensus HA are indicated. (B) The plasmid
    Figure Legend Snippet: The H7N9 HA DNA vaccine design and its expression (A) Phylogenetic tree based on neighbor-joining evaluation of H7HA alignment. Asterisks indicate location of consensus sequence. The strains used to generate the consensus HA are indicated. (B) The plasmid

    Techniques Used: Expressing, Sequencing, Plasmid Preparation

    Related Articles

    Expressing:

    Article Title: Protective Immunity to H7N9 Influenza viruses elicited by synthetic DNA Vaccine
    Article Snippet: The synthetic H7HA gene, which is 1683 bp in length, was synthesized, sequence verified, and was subcloned into the expression vector pGX0001 at BamHI and NotI sites and named pH7HA ( ) in just a few days. .. To determine the expression of the H7HA construct, an indirect immunofluorescent assay was carried out using an anti-H7N9 HA mouse monoclonal antibody. .. High membrane expression was observed by fluorescent microscopy in the pH7HA-transfected cells , supporting the idea that the expressed HA protein was a surface protein and the protein exhibited a relatively native conformation.

    Article Title: Antiviral activity of carnosic acid against respiratory syncytial virus
    Article Snippet: Final concentration of DMSO was 2% in all samples. .. After blocking with 5% skim milk, the expression of hRSV fusion (F) protein was assessed by enzyme-linked immune sorbent assay (ELISA) using mouse anti-F monoclonal antibody (Sino Biological) and horseradish peroxidase (HRP)-conjugated anti-mouse IgG. .. Extraction and isolation of compounds Air-dried, powdered aerial parts of R. officinalis (12.5 g) were extracted three times with 70% ethanol (EtOH) by maceration.

    Construct:

    Article Title: Protective Immunity to H7N9 Influenza viruses elicited by synthetic DNA Vaccine
    Article Snippet: The synthetic H7HA gene, which is 1683 bp in length, was synthesized, sequence verified, and was subcloned into the expression vector pGX0001 at BamHI and NotI sites and named pH7HA ( ) in just a few days. .. To determine the expression of the H7HA construct, an indirect immunofluorescent assay was carried out using an anti-H7N9 HA mouse monoclonal antibody. .. High membrane expression was observed by fluorescent microscopy in the pH7HA-transfected cells , supporting the idea that the expressed HA protein was a surface protein and the protein exhibited a relatively native conformation.

    other:

    Article Title: Rapid and Quantitative Detection of Avian Influenza A(H7N9) Virions in Complex Matrices Based on Combined Magnetic Capture and Quantum Dot Labeling.
    Article Snippet: Avian influenza A(H7N9) virus, which emerged in China in the spring of 2013, has infected hundreds of people and resulted in many deaths.

    Incubation:

    Article Title: Preclinical and Clinical Demonstration of Immunogenicity by mRNA Vaccines against H10N8 and H7N9 Influenza Viruses
    Article Snippet: Blots were incubated with either anti-H10 HA polyclonal antibody (rabbit, 11693; Sino Biological) or anti-H7 HA monoclonal antibody (mouse, 11082-MM04; Sino Biological) overnight at 4°C. .. Blots were incubated with either anti-H10 HA polyclonal antibody (rabbit, 11693; Sino Biological) or anti-H7 HA monoclonal antibody (mouse, 11082-MM04; Sino Biological) overnight at 4°C. .. Included as positive controls were 0.5 μg recombinant H10 HA protein (1505-001; IBT) and recombinant H7 HA protein (1502-001; IBT).

    Article Title: Nanodiamond enhances immune responses in mice against recombinant HA/H7N9 protein
    Article Snippet: ELISA For testing mouse sera, microtiter plates (ImmunoPlate Maxisorp, Nalgen Nunc International, Roskilde, Denmark) were coated with 100 µL of 50 ng H7 (Influenza Antigen A/Anhui/1/2013 (H7N9), NIBSC) in PBS (100 mM NaCl, 32 mM Na2 HPO4 , 17 mM Na2 HPO4, pH 7.2) and incubated overnight. .. After blocking with 3% (w/v) bovine serum albumin (BSA), 0.05% (v/v) Tween20 in PBS (PBST) for 2 h, 100 µL of the specific mouse sera dilution [5000 times in 1% (w/v) BSA in PBST] and an anti H7N9 haemagglutinin/HA monoclonal mouse antibody (SinoBiological InC.) at concentrations of 0.5, 0.75, 1, 1.25, 2.5, 5, 12.5, 25, 50, 100, 150 μg/mL was applied on each ELISA plate and incubated at 25 °C for 1 h. Each serum dilution was measured in triplicate. .. The plates were washed 5 times with PBST, followed by the addition of 100 µl of a goat anti-mouse IgG conjugate horseradish peroxidase (HRP) dilution (2000 times in 1% (w/v) BSA in PBST and then incubated at 25 °C for 1 h. The enzymatic substrate, 1-Step™ Ultra TMB-ELISA Substrate Solution (Thermo Fisher Scientific, Lithuania) was added.

    Blocking Assay:

    Article Title: Nanodiamond enhances immune responses in mice against recombinant HA/H7N9 protein
    Article Snippet: ELISA For testing mouse sera, microtiter plates (ImmunoPlate Maxisorp, Nalgen Nunc International, Roskilde, Denmark) were coated with 100 µL of 50 ng H7 (Influenza Antigen A/Anhui/1/2013 (H7N9), NIBSC) in PBS (100 mM NaCl, 32 mM Na2 HPO4 , 17 mM Na2 HPO4, pH 7.2) and incubated overnight. .. After blocking with 3% (w/v) bovine serum albumin (BSA), 0.05% (v/v) Tween20 in PBS (PBST) for 2 h, 100 µL of the specific mouse sera dilution [5000 times in 1% (w/v) BSA in PBST] and an anti H7N9 haemagglutinin/HA monoclonal mouse antibody (SinoBiological InC.) at concentrations of 0.5, 0.75, 1, 1.25, 2.5, 5, 12.5, 25, 50, 100, 150 μg/mL was applied on each ELISA plate and incubated at 25 °C for 1 h. Each serum dilution was measured in triplicate. .. The plates were washed 5 times with PBST, followed by the addition of 100 µl of a goat anti-mouse IgG conjugate horseradish peroxidase (HRP) dilution (2000 times in 1% (w/v) BSA in PBST and then incubated at 25 °C for 1 h. The enzymatic substrate, 1-Step™ Ultra TMB-ELISA Substrate Solution (Thermo Fisher Scientific, Lithuania) was added.

    Article Title: Antiviral activity of carnosic acid against respiratory syncytial virus
    Article Snippet: Final concentration of DMSO was 2% in all samples. .. After blocking with 5% skim milk, the expression of hRSV fusion (F) protein was assessed by enzyme-linked immune sorbent assay (ELISA) using mouse anti-F monoclonal antibody (Sino Biological) and horseradish peroxidase (HRP)-conjugated anti-mouse IgG. .. Extraction and isolation of compounds Air-dried, powdered aerial parts of R. officinalis (12.5 g) were extracted three times with 70% ethanol (EtOH) by maceration.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Nanodiamond enhances immune responses in mice against recombinant HA/H7N9 protein
    Article Snippet: ELISA For testing mouse sera, microtiter plates (ImmunoPlate Maxisorp, Nalgen Nunc International, Roskilde, Denmark) were coated with 100 µL of 50 ng H7 (Influenza Antigen A/Anhui/1/2013 (H7N9), NIBSC) in PBS (100 mM NaCl, 32 mM Na2 HPO4 , 17 mM Na2 HPO4, pH 7.2) and incubated overnight. .. After blocking with 3% (w/v) bovine serum albumin (BSA), 0.05% (v/v) Tween20 in PBS (PBST) for 2 h, 100 µL of the specific mouse sera dilution [5000 times in 1% (w/v) BSA in PBST] and an anti H7N9 haemagglutinin/HA monoclonal mouse antibody (SinoBiological InC.) at concentrations of 0.5, 0.75, 1, 1.25, 2.5, 5, 12.5, 25, 50, 100, 150 μg/mL was applied on each ELISA plate and incubated at 25 °C for 1 h. Each serum dilution was measured in triplicate. .. The plates were washed 5 times with PBST, followed by the addition of 100 µl of a goat anti-mouse IgG conjugate horseradish peroxidase (HRP) dilution (2000 times in 1% (w/v) BSA in PBST and then incubated at 25 °C for 1 h. The enzymatic substrate, 1-Step™ Ultra TMB-ELISA Substrate Solution (Thermo Fisher Scientific, Lithuania) was added.

    Article Title: Antiviral activity of carnosic acid against respiratory syncytial virus
    Article Snippet: Final concentration of DMSO was 2% in all samples. .. After blocking with 5% skim milk, the expression of hRSV fusion (F) protein was assessed by enzyme-linked immune sorbent assay (ELISA) using mouse anti-F monoclonal antibody (Sino Biological) and horseradish peroxidase (HRP)-conjugated anti-mouse IgG. .. Extraction and isolation of compounds Air-dried, powdered aerial parts of R. officinalis (12.5 g) were extracted three times with 70% ethanol (EtOH) by maceration.

    Staining:

    Article Title: Development and application of a higher throughput RSV plaque assay by immunofluorescent imaging.
    Article Snippet: The plates were allowed to dry for 20min before washing with PBS supplemented with 0.05% Tween-20 (PBST). .. Fixed cells were stained with an either a mixture of an in-house generated mouse anti-F monoclonal antibody (1.25 μg/ml) and an in-house generated mouse anti-N (nucleoprotein) monoclonal antibody (1.25 μg/ml) or a rabbit anti-F monoclonal antibody (Sino Biologics). .. Primary antibodies were incubated with the fixed cells for 1 h before anti-mouse or anti-rabbit IgG Alex488 conjugated secondary antibodies (Invitrogen) was added (1:500 diluted).

    Generated:

    Article Title: Development and application of a higher throughput RSV plaque assay by immunofluorescent imaging.
    Article Snippet: The plates were allowed to dry for 20min before washing with PBS supplemented with 0.05% Tween-20 (PBST). .. Fixed cells were stained with an either a mixture of an in-house generated mouse anti-F monoclonal antibody (1.25 μg/ml) and an in-house generated mouse anti-N (nucleoprotein) monoclonal antibody (1.25 μg/ml) or a rabbit anti-F monoclonal antibody (Sino Biologics). .. Primary antibodies were incubated with the fixed cells for 1 h before anti-mouse or anti-rabbit IgG Alex488 conjugated secondary antibodies (Invitrogen) was added (1:500 diluted).

    Recombinant:

    Article Title: Amplified visual immunosensor integrated with nanozyme for ultrasensitive detection of avian influenza virus
    Article Snippet: Recombinant influenza virus A (H1N1) (California) (CLIHA014-2; lot: 813PH1N1CA) was purchased from Cedarlane (Ontario, Canada). .. Influenza A (H5N2) hemagglutinin antibodies (Anti-H3N2 antibodies HA MAb, Lot: HB05AP2609), Influenza A (H7N9) hemagglutinin antibodies (Anti-H7N9 antibody HA MAb, Lot: HB05JA1903), recombinant influenza virus A (H5N2) HA1 (A/Ostrich/South Africa/A/109/2006)(lot: LC09AP1021), recombinant influenza virus A (H7N8) HA1 (A/Mallard/Netherlands/33/2006) (lot: LC09AP1323) and recombinant influenza virus A (H7N9) HA1 (A/Shanghai/1/2013) (lot: LC09JA2702) were purchased from Sino Biological, Inc. (Beijing, China). .. Anti-H4 (A/environment/Maryland/1101/06)(H4N6) polyclonal antibody was purchased from MyBioSource Inc., San Diago, USA.

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  • 85
    Sino Biological anti h7n9 ha mouse monoclonal antibody
    Robust HA-specific IgG antibody titers and hemagglutination-inhibition titers in the sera of the immunized mice. (A) IgG antibodies against <t>H7N9</t> A/Shanghai/1/2013 influenza HA protein. (B) IgG antibodies against H7N9 A/Anhui/1/2013 influenza HA protein.
    Anti H7n9 Ha Mouse Monoclonal Antibody, supplied by Sino Biological, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti h7n9 ha mouse monoclonal antibody/product/Sino Biological
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti h7n9 ha mouse monoclonal antibody - by Bioz Stars, 2021-02
    85/100 stars
      Buy from Supplier

    92
    Sino Biological influenza a h7n9 hemagglutinin antibodies
    Robust HA-specific IgG antibody titers and hemagglutination-inhibition titers in the sera of the immunized mice. (A) IgG antibodies against <t>H7N9</t> A/Shanghai/1/2013 influenza HA protein. (B) IgG antibodies against H7N9 A/Anhui/1/2013 influenza HA protein.
    Influenza A H7n9 Hemagglutinin Antibodies, supplied by Sino Biological, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/influenza a h7n9 hemagglutinin antibodies/product/Sino Biological
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    influenza a h7n9 hemagglutinin antibodies - by Bioz Stars, 2021-02
    92/100 stars
      Buy from Supplier

    Image Search Results


    Robust HA-specific IgG antibody titers and hemagglutination-inhibition titers in the sera of the immunized mice. (A) IgG antibodies against H7N9 A/Shanghai/1/2013 influenza HA protein. (B) IgG antibodies against H7N9 A/Anhui/1/2013 influenza HA protein.

    Journal: Vaccine

    Article Title: Protective Immunity to H7N9 Influenza viruses elicited by synthetic DNA Vaccine

    doi: 10.1016/j.vaccine.2014.02.038

    Figure Lengend Snippet: Robust HA-specific IgG antibody titers and hemagglutination-inhibition titers in the sera of the immunized mice. (A) IgG antibodies against H7N9 A/Shanghai/1/2013 influenza HA protein. (B) IgG antibodies against H7N9 A/Anhui/1/2013 influenza HA protein.

    Article Snippet: To determine the expression of the H7HA construct, an indirect immunofluorescent assay was carried out using an anti-H7N9 HA mouse monoclonal antibody.

    Techniques: HI Assay, Mouse Assay

    Protection from H7N9 A/Anhui/1/2013 virus challenge in the immunized mice. (A) Experimental schedule of challenge study. The mice (n=10) were immunized with 25 μg of pH7HA twice, three weeks apart. Four weeks after the second immunization, the

    Journal: Vaccine

    Article Title: Protective Immunity to H7N9 Influenza viruses elicited by synthetic DNA Vaccine

    doi: 10.1016/j.vaccine.2014.02.038

    Figure Lengend Snippet: Protection from H7N9 A/Anhui/1/2013 virus challenge in the immunized mice. (A) Experimental schedule of challenge study. The mice (n=10) were immunized with 25 μg of pH7HA twice, three weeks apart. Four weeks after the second immunization, the

    Article Snippet: To determine the expression of the H7HA construct, an indirect immunofluorescent assay was carried out using an anti-H7N9 HA mouse monoclonal antibody.

    Techniques: Mouse Assay

    The H7N9 HA DNA vaccine design and its expression (A) Phylogenetic tree based on neighbor-joining evaluation of H7HA alignment. Asterisks indicate location of consensus sequence. The strains used to generate the consensus HA are indicated. (B) The plasmid

    Journal: Vaccine

    Article Title: Protective Immunity to H7N9 Influenza viruses elicited by synthetic DNA Vaccine

    doi: 10.1016/j.vaccine.2014.02.038

    Figure Lengend Snippet: The H7N9 HA DNA vaccine design and its expression (A) Phylogenetic tree based on neighbor-joining evaluation of H7HA alignment. Asterisks indicate location of consensus sequence. The strains used to generate the consensus HA are indicated. (B) The plasmid

    Article Snippet: To determine the expression of the H7HA construct, an indirect immunofluorescent assay was carried out using an anti-H7N9 HA mouse monoclonal antibody.

    Techniques: Expressing, Sequencing, Plasmid Preparation