spike s2 protein  (Sino Biological)


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    Name:
    MERS CoV Spike S2 Protein
    Description:
    A DNA sequence encoding the spike protein S2 Subunit MERS CoV AFS88936 1 Asp726 Pro1296 was fused with a polyhistidine tag at the C terminus
    Catalog Number:
    40070-V08B
    Price:
    None
    Category:
    recombinant protein
    Product Aliases:
    coronavirus s1 Protein MERS-CoV, coronavirus s2 Protein MERS-CoV, coronavirus spike Protein MERS-CoV, cov spike Protein MERS-CoV, ncov RBD Protein MERS-CoV, ncov s1 Protein MERS-CoV, ncov s2 Protein MERS-CoV, ncov spike Protein MERS-CoV, RBD Protein MERS-CoV, S Protein MERS-CoV, s1 Protein MERS-CoV, Spike RBD Protein MERS-CoV
    Host:
    Baculovirus-Insect Cells
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    Structured Review

    Sino Biological spike s2 protein
    A DNA sequence encoding the spike protein S2 Subunit MERS CoV AFS88936 1 Asp726 Pro1296 was fused with a polyhistidine tag at the C terminus
    https://www.bioz.com/result/spike s2 protein/product/Sino Biological
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    spike s2 protein - by Bioz Stars, 2021-04
    93/100 stars

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    Article Title: SARS‐CoV‐2 surveillance for a non‐human primate breeding research facility, et al. SARS‐CoV‐2 surveillance for a non‐human primate breeding research facility
    Article Snippet: The spike S1 protein contains the receptor‐binding domain that has affinity to the angiotensin‐converting enzyme 2 region, and the spike S2 protein contains the fusion machinery and is anchored to the virus membrane., The plate also includes wells coated with an uninfected cell control antigen.

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  • 93
    Sino Biological mers cov antigen
    <t>MERS-CoV</t> viral RNA in respiratory tissues of llamas (A) and pigs (B). Viral RNA was determined in tissue homogenates at postinoculation days 4 and 24. Error bars indicate SDs when results were positive in > 1 animal. Dashed lines depict the detection limit of the assays (C t ≤40). C t , cycle threshold; MERS-CoV, Middle East respiratory syndrome coronavirus; PI, postinoculation.
    Mers Cov Antigen, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mers cov antigen/product/Sino Biological
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mers cov antigen - by Bioz Stars, 2021-04
    93/100 stars
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    93
    Sino Biological mers cov anti spike antibody
    Sequencing of <t>MERS-CoV</t> Spike protein and data analysis
    Mers Cov Anti Spike Antibody, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mers cov anti spike antibody/product/Sino Biological
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mers cov anti spike antibody - by Bioz Stars, 2021-04
    93/100 stars
      Buy from Supplier

    93
    Sino Biological mers cov spike protein s2 antibody mouse mab
    Incorporation of SARS-CoV-2 S protein into pseudovirions. a Diagram of full-length SARS-CoV-2 S protein with a 3xFLAG tag. S1, receptor-binding subunit; S2, membrane fusion subunit; TM, transmembrane domain; NTD, N-terminal domain; pFP, potential fusion peptide; HR-N, heptad repeat-N; HR-C, heptad repeat-C; b – f Detection of CoVs S protein in cells lysate by western blot. Mock, 293T cells transfected with empty vector. b Mouse monoclonal anti-FLAG M2 antibody; c Polyclonal goat anti-MHV-A59 S protein antibody AO4. d Polyclonal rabbit anti-SARS S1 antibodies T62. e Mouse monoclonal anti-SARS S1 antibody. f Mouse monoclonal <t>anti-MERS-CoV</t> S2 antibody. g – j Detection of CoVs S protein in pseudovirions by western blot.Gag-p24 served as a loading control. g Anti-FLAG M2. h Polyclonal goat anti-MHV-A59 S protein antibody AO4. i Polyclonal rabbit anti-SARS S1 antibodies T62. j Polyclonal anti-Gag-p24 antibodies. uncleaved S protein, about 180 kDa; cleaved S protein, about 90 kDa. Experiments were done twice and one is shown. Source data are provided as a Source Data file.
    Mers Cov Spike Protein S2 Antibody Mouse Mab, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mers cov spike protein s2 antibody mouse mab/product/Sino Biological
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mers cov spike protein s2 antibody mouse mab - by Bioz Stars, 2021-04
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    Image Search Results


    MERS-CoV viral RNA in respiratory tissues of llamas (A) and pigs (B). Viral RNA was determined in tissue homogenates at postinoculation days 4 and 24. Error bars indicate SDs when results were positive in > 1 animal. Dashed lines depict the detection limit of the assays (C t ≤40). C t , cycle threshold; MERS-CoV, Middle East respiratory syndrome coronavirus; PI, postinoculation.

    Journal: Emerging Infectious Diseases

    Article Title: Livestock Susceptibility to Infection with Middle East Respiratory Syndrome Coronavirus

    doi: 10.3201/eid2302.161239

    Figure Lengend Snippet: MERS-CoV viral RNA in respiratory tissues of llamas (A) and pigs (B). Viral RNA was determined in tissue homogenates at postinoculation days 4 and 24. Error bars indicate SDs when results were positive in > 1 animal. Dashed lines depict the detection limit of the assays (C t ≤40). C t , cycle threshold; MERS-CoV, Middle East respiratory syndrome coronavirus; PI, postinoculation.

    Article Snippet: Sequential slides were either stained with hematoxylin and eosin or used to detect the DPP4 receptor and MERS-CoV antigen by IHC and viral genome by ISH ( , ).

    Techniques:

    Antibody responses after experimental inoculation of MERS-CoV into llamas and pigs. A) MERS-CoV S1 antibody responses were analyzed in serum from all animals at postinoculation days 0, 14, and 24. An ELISA with recombinant MERS-CoV S1 protein was used, and results are represented individually. B) Individual MERS-CoV neutralization titers from llamas and pigs as determined from serum. Dashed lines depict the detection limit of the assays. MERS-CoV, Middle East respiratory syndrome coronavirus; OD, optical density; PRNT 90 , 90% plaque reduction neutralization test.

    Journal: Emerging Infectious Diseases

    Article Title: Livestock Susceptibility to Infection with Middle East Respiratory Syndrome Coronavirus

    doi: 10.3201/eid2302.161239

    Figure Lengend Snippet: Antibody responses after experimental inoculation of MERS-CoV into llamas and pigs. A) MERS-CoV S1 antibody responses were analyzed in serum from all animals at postinoculation days 0, 14, and 24. An ELISA with recombinant MERS-CoV S1 protein was used, and results are represented individually. B) Individual MERS-CoV neutralization titers from llamas and pigs as determined from serum. Dashed lines depict the detection limit of the assays. MERS-CoV, Middle East respiratory syndrome coronavirus; OD, optical density; PRNT 90 , 90% plaque reduction neutralization test.

    Article Snippet: Sequential slides were either stained with hematoxylin and eosin or used to detect the DPP4 receptor and MERS-CoV antigen by IHC and viral genome by ISH ( , ).

    Techniques: Enzyme-linked Immunosorbent Assay, Recombinant, Neutralization, Plaque Reduction Neutralization Test

    Viral shedding of llamas and pigs after experimental inoculation with MERS-CoV. A) Viral RNA and B) infectious MERS-CoV from nasal swab samples collected from llamas (top) and pigs (bottom) at different times after challenge. Each bar represents an individual animal. Dashed lines depict the detection limit of the assays. C t , cycle threshold; MERS-CoV, Middle East respiratory syndrome coronavirus; TCID 50 , 50% tissue culture infective dose.

    Journal: Emerging Infectious Diseases

    Article Title: Livestock Susceptibility to Infection with Middle East Respiratory Syndrome Coronavirus

    doi: 10.3201/eid2302.161239

    Figure Lengend Snippet: Viral shedding of llamas and pigs after experimental inoculation with MERS-CoV. A) Viral RNA and B) infectious MERS-CoV from nasal swab samples collected from llamas (top) and pigs (bottom) at different times after challenge. Each bar represents an individual animal. Dashed lines depict the detection limit of the assays. C t , cycle threshold; MERS-CoV, Middle East respiratory syndrome coronavirus; TCID 50 , 50% tissue culture infective dose.

    Article Snippet: Sequential slides were either stained with hematoxylin and eosin or used to detect the DPP4 receptor and MERS-CoV antigen by IHC and viral genome by ISH ( , ).

    Techniques:

    Histology and expression of viral antigen (IHC) and viral RNA (ISH) at postinoculation day 4 in the nasal respiratory epithelium of sheep, pigs, llamas, and horses inoculated with MERS-CoV. A mild to severe rhinitis with epithelial necrosis and hypertrophy and inflammation of the epithelium and lamina propria was observed in the nasal respiratory tissue of pigs and llamas. Associated with these was presence of virus antigen (IHC) and RNA (ISH). No substantial lesions, virus antigen, or virus RNA were detected in the nasal respiratory tissues of sheep and horses (HE, IHC, ISH). Original magnification ×200 for all images. HE, hematoxylin and eosin; IHC, immunohistochemistry; ISH, in situ hybridization; MERS-CoV, Middle East respiratory syndrome coronavirus.

    Journal: Emerging Infectious Diseases

    Article Title: Livestock Susceptibility to Infection with Middle East Respiratory Syndrome Coronavirus

    doi: 10.3201/eid2302.161239

    Figure Lengend Snippet: Histology and expression of viral antigen (IHC) and viral RNA (ISH) at postinoculation day 4 in the nasal respiratory epithelium of sheep, pigs, llamas, and horses inoculated with MERS-CoV. A mild to severe rhinitis with epithelial necrosis and hypertrophy and inflammation of the epithelium and lamina propria was observed in the nasal respiratory tissue of pigs and llamas. Associated with these was presence of virus antigen (IHC) and RNA (ISH). No substantial lesions, virus antigen, or virus RNA were detected in the nasal respiratory tissues of sheep and horses (HE, IHC, ISH). Original magnification ×200 for all images. HE, hematoxylin and eosin; IHC, immunohistochemistry; ISH, in situ hybridization; MERS-CoV, Middle East respiratory syndrome coronavirus.

    Article Snippet: Sequential slides were either stained with hematoxylin and eosin or used to detect the DPP4 receptor and MERS-CoV antigen by IHC and viral genome by ISH ( , ).

    Techniques: Expressing, Immunohistochemistry, In Situ Hybridization

    Presence of MERS-CoV receptor DPP4 (IHC) and of mucosubstances (PAS) in upper and lower respiratory tract tissues from sheep, pigs, llamas, and horses. A) In the nose, DPP4 (red cytoplasmic or membrane staining) was present on the lining epithelium of pigs, llamas, and horses but not sheep. PAS staining (magenta) demonstrated more mucous cells in the lining epithelium of sheep and horses and a layer of mucus on the lining epithelium of the horses. B) DPP4 (red cytoplasmic or membrane staining) was present on the lining epithelium of the trachea, bronchus/bronchioles, and alveoli in the pigs, llamas and horses but not in the sheep. Original magnification ×400 for all images. DPP4, dipeptidyl peptidase-4; IHC, immunohistochemistry; MERS-CoV, Middle East respiratory syndrome coronavirus; PAS, periodic acid–Schiff; term., terminal.

    Journal: Emerging Infectious Diseases

    Article Title: Livestock Susceptibility to Infection with Middle East Respiratory Syndrome Coronavirus

    doi: 10.3201/eid2302.161239

    Figure Lengend Snippet: Presence of MERS-CoV receptor DPP4 (IHC) and of mucosubstances (PAS) in upper and lower respiratory tract tissues from sheep, pigs, llamas, and horses. A) In the nose, DPP4 (red cytoplasmic or membrane staining) was present on the lining epithelium of pigs, llamas, and horses but not sheep. PAS staining (magenta) demonstrated more mucous cells in the lining epithelium of sheep and horses and a layer of mucus on the lining epithelium of the horses. B) DPP4 (red cytoplasmic or membrane staining) was present on the lining epithelium of the trachea, bronchus/bronchioles, and alveoli in the pigs, llamas and horses but not in the sheep. Original magnification ×400 for all images. DPP4, dipeptidyl peptidase-4; IHC, immunohistochemistry; MERS-CoV, Middle East respiratory syndrome coronavirus; PAS, periodic acid–Schiff; term., terminal.

    Article Snippet: Sequential slides were either stained with hematoxylin and eosin or used to detect the DPP4 receptor and MERS-CoV antigen by IHC and viral genome by ISH ( , ).

    Techniques: Immunohistochemistry, Staining

    Single-dose intranasal immunization with PIV5-MERS-S induced robust MERS-CoV-specific CD8 T cell response in human DPP4 knockin (hDPP4 KI) mice. (A) Schematic diagram showing the experimental plan to examine CD8 T cell response after immunization and challenge. hDPP4 KI mice were intranasally immunized with 10 4 PFU PIV5-MERS-S or PIV5-GFP. At 4 weeks or 4 days following immunization, mice were challenged with MERS MA 6.1.2, single-cell suspensions from the lungs of immunized mice were stimulated with MERS-CoV spike peptides (S343 and S1165), and specific CD8 T cells were determined by IFN-γ intracellular staining. (B to D) Representative FACS plots (B), percentage (C), and total number (D) of MERS-CoV-specific CD8 T cells in the lungs at 4 weeks after immunization. (E to G) Representative FACS plots (E), percentage (F), and total number (G) of MERS-CoV-specific CD8 T cells in the lungs at 4 days after MERS MA 6.1.2 challenge ( n = 6 mice per group). Data are representative of two independent experiments. Data presented represent mean ± SE; * denotes P

    Journal: mBio

    Article Title: Single-Dose, Intranasal Immunization with Recombinant Parainfluenza Virus 5 Expressing Middle East Respiratory Syndrome Coronavirus (MERS-CoV) Spike Protein Protects Mice from Fatal MERS-CoV Infection

    doi: 10.1128/mBio.00554-20

    Figure Lengend Snippet: Single-dose intranasal immunization with PIV5-MERS-S induced robust MERS-CoV-specific CD8 T cell response in human DPP4 knockin (hDPP4 KI) mice. (A) Schematic diagram showing the experimental plan to examine CD8 T cell response after immunization and challenge. hDPP4 KI mice were intranasally immunized with 10 4 PFU PIV5-MERS-S or PIV5-GFP. At 4 weeks or 4 days following immunization, mice were challenged with MERS MA 6.1.2, single-cell suspensions from the lungs of immunized mice were stimulated with MERS-CoV spike peptides (S343 and S1165), and specific CD8 T cells were determined by IFN-γ intracellular staining. (B to D) Representative FACS plots (B), percentage (C), and total number (D) of MERS-CoV-specific CD8 T cells in the lungs at 4 weeks after immunization. (E to G) Representative FACS plots (E), percentage (F), and total number (G) of MERS-CoV-specific CD8 T cells in the lungs at 4 days after MERS MA 6.1.2 challenge ( n = 6 mice per group). Data are representative of two independent experiments. Data presented represent mean ± SE; * denotes P

    Article Snippet: The expression of MERS-CoV spike protein was detected by a rabbit anti-MERS-CoV S2 antibody (Sino Biological, catalog no. 40070-T60) and colocalized using a mouse anti-α-tubulin antibody (Sigma, catalog no. T9026).

    Techniques: Knock-In, Mouse Assay, Staining, FACS

    Serum neutralizing antibodies produced in mice 4 weeks after single-dose intranasal immunization with PIV5-MERS-S. Naive mice were intranasally immunized with PIV5-GFP or PIV5-MERS-S. Sera were collected at 4 weeks postimmunization. Neutralization assay against MERS-CoV spike pseudovirions was performed as described in Materials and Methods. The neutralization results were measured in luciferase activity and plotted relative to mock-treatment value. (A and B) Neutralization assay results from C57BL/6 mice immunized with 10 4 PFU (A) and 10 6 PFU (B) PIV5-MERS-S or PIV5-GFP. (C) Neutralization assay results from BALB/c mice immunized with 10 6 PFU PIV5-MERS-S or PIV5-GFP. Data presented represent means ± SEs.

    Journal: mBio

    Article Title: Single-Dose, Intranasal Immunization with Recombinant Parainfluenza Virus 5 Expressing Middle East Respiratory Syndrome Coronavirus (MERS-CoV) Spike Protein Protects Mice from Fatal MERS-CoV Infection

    doi: 10.1128/mBio.00554-20

    Figure Lengend Snippet: Serum neutralizing antibodies produced in mice 4 weeks after single-dose intranasal immunization with PIV5-MERS-S. Naive mice were intranasally immunized with PIV5-GFP or PIV5-MERS-S. Sera were collected at 4 weeks postimmunization. Neutralization assay against MERS-CoV spike pseudovirions was performed as described in Materials and Methods. The neutralization results were measured in luciferase activity and plotted relative to mock-treatment value. (A and B) Neutralization assay results from C57BL/6 mice immunized with 10 4 PFU (A) and 10 6 PFU (B) PIV5-MERS-S or PIV5-GFP. (C) Neutralization assay results from BALB/c mice immunized with 10 6 PFU PIV5-MERS-S or PIV5-GFP. Data presented represent means ± SEs.

    Article Snippet: The expression of MERS-CoV spike protein was detected by a rabbit anti-MERS-CoV S2 antibody (Sino Biological, catalog no. 40070-T60) and colocalized using a mouse anti-α-tubulin antibody (Sigma, catalog no. T9026).

    Techniques: Produced, Mouse Assay, Neutralization, Luciferase, Activity Assay

    Representative images of lung tissues from mice receiving PBS (A), UV-inactivated MERS-CoV (B), or PIV5-MERS-S (C) treatment, followed by infection with MERS MA 6.1.2. Images obtained from tissues at 3 days after MERS MA 6.1.2 infection. Compared to PBS or PIV5-MERS, perivascular eosinophilic infiltration (arrows) in UV-MERS-treated mice was greatly increased. n = 3 to 4 mice/group. (D) Graph representing eosinophil infiltration in the lung tissues of mice from groups shown in panels A to . * denotes P

    Journal: mBio

    Article Title: Single-Dose, Intranasal Immunization with Recombinant Parainfluenza Virus 5 Expressing Middle East Respiratory Syndrome Coronavirus (MERS-CoV) Spike Protein Protects Mice from Fatal MERS-CoV Infection

    doi: 10.1128/mBio.00554-20

    Figure Lengend Snippet: Representative images of lung tissues from mice receiving PBS (A), UV-inactivated MERS-CoV (B), or PIV5-MERS-S (C) treatment, followed by infection with MERS MA 6.1.2. Images obtained from tissues at 3 days after MERS MA 6.1.2 infection. Compared to PBS or PIV5-MERS, perivascular eosinophilic infiltration (arrows) in UV-MERS-treated mice was greatly increased. n = 3 to 4 mice/group. (D) Graph representing eosinophil infiltration in the lung tissues of mice from groups shown in panels A to . * denotes P

    Article Snippet: The expression of MERS-CoV spike protein was detected by a rabbit anti-MERS-CoV S2 antibody (Sino Biological, catalog no. 40070-T60) and colocalized using a mouse anti-α-tubulin antibody (Sigma, catalog no. T9026).

    Techniques: Mouse Assay, Infection

    Histopathology in immunized mice challenged with MERS-CoV. hDPP4 KI mice were intranasally immunized with 10 4 PFU PIV5-MERS-S or PIV5-GFP. Four weeks after immunization, the mice were intranasally infected with 10 5 PFU MERS MA 6.1.2. (A) Representative images of H E-stained lung sections from PIV5-MERS-S- or PIV5-GFP-immunized hDPP4 KI mice at indicated days after MERS MA 6.1.2 challenge. Note the cellular infiltration (black arrows) and the hyaline membranes (red arrows). (B) Summary scores for disease in the lung sections. n = 3 to 5 mice/group. * denotes P

    Journal: mBio

    Article Title: Single-Dose, Intranasal Immunization with Recombinant Parainfluenza Virus 5 Expressing Middle East Respiratory Syndrome Coronavirus (MERS-CoV) Spike Protein Protects Mice from Fatal MERS-CoV Infection

    doi: 10.1128/mBio.00554-20

    Figure Lengend Snippet: Histopathology in immunized mice challenged with MERS-CoV. hDPP4 KI mice were intranasally immunized with 10 4 PFU PIV5-MERS-S or PIV5-GFP. Four weeks after immunization, the mice were intranasally infected with 10 5 PFU MERS MA 6.1.2. (A) Representative images of H E-stained lung sections from PIV5-MERS-S- or PIV5-GFP-immunized hDPP4 KI mice at indicated days after MERS MA 6.1.2 challenge. Note the cellular infiltration (black arrows) and the hyaline membranes (red arrows). (B) Summary scores for disease in the lung sections. n = 3 to 5 mice/group. * denotes P

    Article Snippet: The expression of MERS-CoV spike protein was detected by a rabbit anti-MERS-CoV S2 antibody (Sino Biological, catalog no. 40070-T60) and colocalized using a mouse anti-α-tubulin antibody (Sigma, catalog no. T9026).

    Techniques: Histopathology, Mouse Assay, Infection, Staining

    Comparison of the protective efficacy between single-dose immunization with UV light-inactivated MERS-CoV and PIV5-MERS-S. hDPP4 KI mice were immunized with 10 4 PFU PIV5-MERS-S via intranasal route; 10 6 PFU UV-inactivated MERS MA 6.1.2, mixed with Imject alum; or PBS via intramuscular route. Four weeks after immunization, immunized mice were infected with 10 5 PFU MERS-CoV. (A) Schematic timeline outlining experimental plan. (B and C) Survival (B) and weight loss (C) were monitored daily until 10 days postinfection. PBS, n = 9; UV MERS-CoV, n = 12; PIV5-MERS-S, n = 8. Data represent mean ± SE.

    Journal: mBio

    Article Title: Single-Dose, Intranasal Immunization with Recombinant Parainfluenza Virus 5 Expressing Middle East Respiratory Syndrome Coronavirus (MERS-CoV) Spike Protein Protects Mice from Fatal MERS-CoV Infection

    doi: 10.1128/mBio.00554-20

    Figure Lengend Snippet: Comparison of the protective efficacy between single-dose immunization with UV light-inactivated MERS-CoV and PIV5-MERS-S. hDPP4 KI mice were immunized with 10 4 PFU PIV5-MERS-S via intranasal route; 10 6 PFU UV-inactivated MERS MA 6.1.2, mixed with Imject alum; or PBS via intramuscular route. Four weeks after immunization, immunized mice were infected with 10 5 PFU MERS-CoV. (A) Schematic timeline outlining experimental plan. (B and C) Survival (B) and weight loss (C) were monitored daily until 10 days postinfection. PBS, n = 9; UV MERS-CoV, n = 12; PIV5-MERS-S, n = 8. Data represent mean ± SE.

    Article Snippet: The expression of MERS-CoV spike protein was detected by a rabbit anti-MERS-CoV S2 antibody (Sino Biological, catalog no. 40070-T60) and colocalized using a mouse anti-α-tubulin antibody (Sigma, catalog no. T9026).

    Techniques: Mouse Assay, Infection

    Generation and characterization of recombinant PIV5 expressing MERS-CoV spike protein. (A) Schematic of PIV5-MERS-S. NP, nucleoprotein; V, V protein; P, phosphoprotein; M, matrix protein; F, fusion protein; SH, small hydrophobic protein; HN, hemagglutinin-neuraminidase protein; L, RNA-dependent RNA polymerase. (B) Confirmation of MERS-CoV spike protein expression by Western blotting. Vero 81 cells were infected with PIV5-MERS-S at MOIs of 0.01, 0.1, and 1.0 or mock infected. At 2 days postinfection, MERS-CoV spike was detected with anti-MERS-S antibody by Western blotting. (C) Immunofluorescence of Vero cells infected with PIV5 and PIV5-MERS-S. Vero cells were infected with PIV5 and PIV5-MERS-S (MOI = 0.1) or mock infected. At 2 days postinfection, cells were fixed, permeabilized, and stained with anti-PIV5 V/P or anti-MERS-spike antibodies. Scale bar = 200 μm. (D) Growth rate of PIV5-MERS-S. MDBK cells were infected with PIV5 or PIV5-MERS-S at an MOI of 0.1. Media were collected daily for 5 days, and titers of viruses in the media were determined using plaque assay.

    Journal: mBio

    Article Title: Single-Dose, Intranasal Immunization with Recombinant Parainfluenza Virus 5 Expressing Middle East Respiratory Syndrome Coronavirus (MERS-CoV) Spike Protein Protects Mice from Fatal MERS-CoV Infection

    doi: 10.1128/mBio.00554-20

    Figure Lengend Snippet: Generation and characterization of recombinant PIV5 expressing MERS-CoV spike protein. (A) Schematic of PIV5-MERS-S. NP, nucleoprotein; V, V protein; P, phosphoprotein; M, matrix protein; F, fusion protein; SH, small hydrophobic protein; HN, hemagglutinin-neuraminidase protein; L, RNA-dependent RNA polymerase. (B) Confirmation of MERS-CoV spike protein expression by Western blotting. Vero 81 cells were infected with PIV5-MERS-S at MOIs of 0.01, 0.1, and 1.0 or mock infected. At 2 days postinfection, MERS-CoV spike was detected with anti-MERS-S antibody by Western blotting. (C) Immunofluorescence of Vero cells infected with PIV5 and PIV5-MERS-S. Vero cells were infected with PIV5 and PIV5-MERS-S (MOI = 0.1) or mock infected. At 2 days postinfection, cells were fixed, permeabilized, and stained with anti-PIV5 V/P or anti-MERS-spike antibodies. Scale bar = 200 μm. (D) Growth rate of PIV5-MERS-S. MDBK cells were infected with PIV5 or PIV5-MERS-S at an MOI of 0.1. Media were collected daily for 5 days, and titers of viruses in the media were determined using plaque assay.

    Article Snippet: The expression of MERS-CoV spike protein was detected by a rabbit anti-MERS-CoV S2 antibody (Sino Biological, catalog no. 40070-T60) and colocalized using a mouse anti-α-tubulin antibody (Sigma, catalog no. T9026).

    Techniques: Recombinant, Expressing, Western Blot, Infection, Immunofluorescence, Staining, Plaque Assay

    Single-dose intranasal immunization with PIV5-MERS-S completely protects hDPP4 KI mice from lethal MERS-CoV challenge. (A) Schematic timeline showing immunization, challenge, and the evaluation of protection. hDPP4 KI mice were intranasally immunized with 10 4 PFU PIV5-MERS-S, PIV5-GFP, or PBS. Four weeks after immunization, the mice were intranasally infected with 10 5 PFU MERS MA 6.1.2. (B and C) Survival (B) and weight loss (C) were monitored daily for 12 days. PIV5-MERS-S or PIV5-GFP, n = 10; PBS, n = 5. (D) At indicated days postinfection, virus lung titers were quantified by plaque assay. Data are representative of two independent experiments. Data presented represent mean ± SE; * denotes P

    Journal: mBio

    Article Title: Single-Dose, Intranasal Immunization with Recombinant Parainfluenza Virus 5 Expressing Middle East Respiratory Syndrome Coronavirus (MERS-CoV) Spike Protein Protects Mice from Fatal MERS-CoV Infection

    doi: 10.1128/mBio.00554-20

    Figure Lengend Snippet: Single-dose intranasal immunization with PIV5-MERS-S completely protects hDPP4 KI mice from lethal MERS-CoV challenge. (A) Schematic timeline showing immunization, challenge, and the evaluation of protection. hDPP4 KI mice were intranasally immunized with 10 4 PFU PIV5-MERS-S, PIV5-GFP, or PBS. Four weeks after immunization, the mice were intranasally infected with 10 5 PFU MERS MA 6.1.2. (B and C) Survival (B) and weight loss (C) were monitored daily for 12 days. PIV5-MERS-S or PIV5-GFP, n = 10; PBS, n = 5. (D) At indicated days postinfection, virus lung titers were quantified by plaque assay. Data are representative of two independent experiments. Data presented represent mean ± SE; * denotes P

    Article Snippet: The expression of MERS-CoV spike protein was detected by a rabbit anti-MERS-CoV S2 antibody (Sino Biological, catalog no. 40070-T60) and colocalized using a mouse anti-α-tubulin antibody (Sigma, catalog no. T9026).

    Techniques: Mouse Assay, Infection, Plaque Assay

    Sequencing of MERS-CoV Spike protein and data analysis

    Journal: Virology

    Article Title: Intratracheal exposure of common marmosets to MERS-CoV Jordan-n3/2012 or MERS-CoV EMC/2012 isolates does not result in lethal disease

    doi: 10.1016/j.virol.2015.07.013

    Figure Lengend Snippet: Sequencing of MERS-CoV Spike protein and data analysis

    Article Snippet: MERS-CoV infection was visualized by fluorescence using a MERS-CoV anti-Spike antibody (Sino Biological) on an Operetta High Content Imager (Perkin-Elmer).

    Techniques: Sequencing

    Gross and Histopathology. (A) Gross lung pathology demonstrating mostly normal lung with multifocal to coalescing moderate interstitial pneumonia in the left caudal lung lobe. (B) Low magnification of lung field from MERS-CoV EMC inoculated subject demonstrating

    Journal: Virology

    Article Title: Intratracheal exposure of common marmosets to MERS-CoV Jordan-n3/2012 or MERS-CoV EMC/2012 isolates does not result in lethal disease

    doi: 10.1016/j.virol.2015.07.013

    Figure Lengend Snippet: Gross and Histopathology. (A) Gross lung pathology demonstrating mostly normal lung with multifocal to coalescing moderate interstitial pneumonia in the left caudal lung lobe. (B) Low magnification of lung field from MERS-CoV EMC inoculated subject demonstrating

    Article Snippet: MERS-CoV infection was visualized by fluorescence using a MERS-CoV anti-Spike antibody (Sino Biological) on an Operetta High Content Imager (Perkin-Elmer).

    Techniques: Histopathology

    Ex-vivo analysis of primary cells. Cells were isolated from lung, kidney, and bronchoalveolar lavage (BAL), and one-step growth kinetics were performed as described in Materials and Methods. Lung, Kidney and BAL demonstrate that MERS-CoV is able to replicate

    Journal: Virology

    Article Title: Intratracheal exposure of common marmosets to MERS-CoV Jordan-n3/2012 or MERS-CoV EMC/2012 isolates does not result in lethal disease

    doi: 10.1016/j.virol.2015.07.013

    Figure Lengend Snippet: Ex-vivo analysis of primary cells. Cells were isolated from lung, kidney, and bronchoalveolar lavage (BAL), and one-step growth kinetics were performed as described in Materials and Methods. Lung, Kidney and BAL demonstrate that MERS-CoV is able to replicate

    Article Snippet: MERS-CoV infection was visualized by fluorescence using a MERS-CoV anti-Spike antibody (Sino Biological) on an Operetta High Content Imager (Perkin-Elmer).

    Techniques: Ex Vivo, Isolation

    Fluorescence Reduction Neutralizing Assay. Sera from all subjects was evaluated for the presence of neutralizing antibody to MERS-CoV as described in Materials and Methods. Neutralizing antibody could be detected above the background of the Mock infected

    Journal: Virology

    Article Title: Intratracheal exposure of common marmosets to MERS-CoV Jordan-n3/2012 or MERS-CoV EMC/2012 isolates does not result in lethal disease

    doi: 10.1016/j.virol.2015.07.013

    Figure Lengend Snippet: Fluorescence Reduction Neutralizing Assay. Sera from all subjects was evaluated for the presence of neutralizing antibody to MERS-CoV as described in Materials and Methods. Neutralizing antibody could be detected above the background of the Mock infected

    Article Snippet: MERS-CoV infection was visualized by fluorescence using a MERS-CoV anti-Spike antibody (Sino Biological) on an Operetta High Content Imager (Perkin-Elmer).

    Techniques: Fluorescence, Neutralizing Assay, Infection

    Incorporation of SARS-CoV-2 S protein into pseudovirions. a Diagram of full-length SARS-CoV-2 S protein with a 3xFLAG tag. S1, receptor-binding subunit; S2, membrane fusion subunit; TM, transmembrane domain; NTD, N-terminal domain; pFP, potential fusion peptide; HR-N, heptad repeat-N; HR-C, heptad repeat-C; b – f Detection of CoVs S protein in cells lysate by western blot. Mock, 293T cells transfected with empty vector. b Mouse monoclonal anti-FLAG M2 antibody; c Polyclonal goat anti-MHV-A59 S protein antibody AO4. d Polyclonal rabbit anti-SARS S1 antibodies T62. e Mouse monoclonal anti-SARS S1 antibody. f Mouse monoclonal anti-MERS-CoV S2 antibody. g – j Detection of CoVs S protein in pseudovirions by western blot.Gag-p24 served as a loading control. g Anti-FLAG M2. h Polyclonal goat anti-MHV-A59 S protein antibody AO4. i Polyclonal rabbit anti-SARS S1 antibodies T62. j Polyclonal anti-Gag-p24 antibodies. uncleaved S protein, about 180 kDa; cleaved S protein, about 90 kDa. Experiments were done twice and one is shown. Source data are provided as a Source Data file.

    Journal: Nature Communications

    Article Title: Characterization of spike glycoprotein of SARS-CoV-2 on virus entry and its immune cross-reactivity with SARS-CoV

    doi: 10.1038/s41467-020-15562-9

    Figure Lengend Snippet: Incorporation of SARS-CoV-2 S protein into pseudovirions. a Diagram of full-length SARS-CoV-2 S protein with a 3xFLAG tag. S1, receptor-binding subunit; S2, membrane fusion subunit; TM, transmembrane domain; NTD, N-terminal domain; pFP, potential fusion peptide; HR-N, heptad repeat-N; HR-C, heptad repeat-C; b – f Detection of CoVs S protein in cells lysate by western blot. Mock, 293T cells transfected with empty vector. b Mouse monoclonal anti-FLAG M2 antibody; c Polyclonal goat anti-MHV-A59 S protein antibody AO4. d Polyclonal rabbit anti-SARS S1 antibodies T62. e Mouse monoclonal anti-SARS S1 antibody. f Mouse monoclonal anti-MERS-CoV S2 antibody. g – j Detection of CoVs S protein in pseudovirions by western blot.Gag-p24 served as a loading control. g Anti-FLAG M2. h Polyclonal goat anti-MHV-A59 S protein antibody AO4. i Polyclonal rabbit anti-SARS S1 antibodies T62. j Polyclonal anti-Gag-p24 antibodies. uncleaved S protein, about 180 kDa; cleaved S protein, about 90 kDa. Experiments were done twice and one is shown. Source data are provided as a Source Data file.

    Article Snippet: Rabbit polyclonal against SARS S1 antibodies (#40150-T62), mouse monoclonal against MERS-CoV S2 antibody (#40070-MM11), mouse monoclonal against SARS S1 antibody (#40150-MM02), rabbit polyclonal against HIV-1 Gag-p24 antibody (11695-RP01) were purchased from Sino Biological Inc. (Beijing, China).

    Techniques: Binding Assay, Western Blot, Transfection, Plasmid Preparation