rabbit anti mers cov antibodies  (Sino Biological)


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    Name:
    MERS CoV Spike Antibody Rabbit MAb
    Description:
    This antibody was obtained from a rabbit immunized with purified recombinant recombinant MERS CoV NCoV Novel coronavirus Spike Protein Catalog 40069 V08B AFS88936 1 Met1 Trp1297
    Catalog Number:
    40069-R723
    Price:
    None
    Category:
    Primary Antibody
    Reactivity:
    MERS CoV
    Applications:
    Microneutralizaiton(MN)
    Immunogen:
    Recombinant MERS-CoV (NCoV / Novel coronavirus) Spike Protein (ECD, aa 1-1297) (Catalog#40069-V08B)
    Product Aliases:
    Anti-coronavirus s1 Antibody, Anti-coronavirus s2 Antibody, Anti-coronavirus spike Antibody, Anti-cov spike Antibody, Anti-ncov RBD Antibody, Anti-ncov s1 Antibody, Anti-ncov s2 Antibody, Anti-ncov spike Antibody, Anti-RBD Antibody, Anti-S Antibody, Anti-s1 Antibody, Anti-Spike RBD Antibody
    Antibody Type:
    MAb
    Host:
    Rabbit
    Isotype:
    Rabbit IgG
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    Structured Review

    Sino Biological rabbit anti mers cov antibodies
    Characterization of <t>anti-MERS-S2P</t> IgGs. ( A ) Immunofluorescence assay of anti-MERS-S2P IgGs on cell lines infected with various human coronaviruses (hCoVs) to determine their <t>MERS-CoV</t> specificity. Scale bar, 200 μm. ( B ) Size-exclusion chromatography analysis of S2A3 (IgG) and S2D5 (IgG). The molecular weights (kDa) of the molecular mass markers are shown above the corresponding peaks at the top chromatogram. ( C ) Surface plasmon resonance (SPR) analysis of S2A3 (IgG) and S2D5 (IgG) on a MERS-S2P-immobilized sensor chip to determine their apparent binding affinities. The fitted-lines are marked by red.
    This antibody was obtained from a rabbit immunized with purified recombinant recombinant MERS CoV NCoV Novel coronavirus Spike Protein Catalog 40069 V08B AFS88936 1 Met1 Trp1297
    https://www.bioz.com/result/rabbit anti mers cov antibodies/product/Sino Biological
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti mers cov antibodies - by Bioz Stars, 2021-04
    95/100 stars

    Images

    1) Product Images from "Selection and Characterization of Monoclonal Antibodies Targeting Middle East Respiratory Syndrome Coronavirus through a Human Synthetic Fab Phage Display Library Panning"

    Article Title: Selection and Characterization of Monoclonal Antibodies Targeting Middle East Respiratory Syndrome Coronavirus through a Human Synthetic Fab Phage Display Library Panning

    Journal: Antibodies

    doi: 10.3390/antib8030042

    Characterization of anti-MERS-S2P IgGs. ( A ) Immunofluorescence assay of anti-MERS-S2P IgGs on cell lines infected with various human coronaviruses (hCoVs) to determine their MERS-CoV specificity. Scale bar, 200 μm. ( B ) Size-exclusion chromatography analysis of S2A3 (IgG) and S2D5 (IgG). The molecular weights (kDa) of the molecular mass markers are shown above the corresponding peaks at the top chromatogram. ( C ) Surface plasmon resonance (SPR) analysis of S2A3 (IgG) and S2D5 (IgG) on a MERS-S2P-immobilized sensor chip to determine their apparent binding affinities. The fitted-lines are marked by red.
    Figure Legend Snippet: Characterization of anti-MERS-S2P IgGs. ( A ) Immunofluorescence assay of anti-MERS-S2P IgGs on cell lines infected with various human coronaviruses (hCoVs) to determine their MERS-CoV specificity. Scale bar, 200 μm. ( B ) Size-exclusion chromatography analysis of S2A3 (IgG) and S2D5 (IgG). The molecular weights (kDa) of the molecular mass markers are shown above the corresponding peaks at the top chromatogram. ( C ) Surface plasmon resonance (SPR) analysis of S2A3 (IgG) and S2D5 (IgG) on a MERS-S2P-immobilized sensor chip to determine their apparent binding affinities. The fitted-lines are marked by red.

    Techniques Used: Immunofluorescence, Infection, Size-exclusion Chromatography, SPR Assay, Chromatin Immunoprecipitation, Binding Assay

    Output of the panning of the phage-displayed synthetic Fab library on MERS-S2P. ( A ) Monitoring of phage titers over three rounds (R1–R3) of panning. Black and gray bars indicate a ratio of phage output to input titers presented as a percentage (%) from panning on MERS-S2P-immobilized and -non-immobilized surfaces, respectively. The ratio of output to input (%) = (phage output titer ÷ phage input titer) × 100. ( B ) Phage ELISAs performed on MERS-S2P-, SARS-CoV spike protein-, a CoV spike protein-immobilized surfaces (blue, red, and green, respectively). ( C ) Amino acid sequences of three unique clones identified from panning (left) and their relative frequencies (%) (right). The sequences were aligned using the Kabat numbering system [ 38 ]. ELISA, enzyme-linked immunosorbent assay; MERS-S2P, Middle East respiratory syndrome-CoV S2 subunit protein; SARS-SP, severe acute respiratory syndrome-CoV S protein; HKU1-SP, hCoV HKU1 S protein; CoV, coronavirus; CDR, complementarity-determining region; FR, framework region.
    Figure Legend Snippet: Output of the panning of the phage-displayed synthetic Fab library on MERS-S2P. ( A ) Monitoring of phage titers over three rounds (R1–R3) of panning. Black and gray bars indicate a ratio of phage output to input titers presented as a percentage (%) from panning on MERS-S2P-immobilized and -non-immobilized surfaces, respectively. The ratio of output to input (%) = (phage output titer ÷ phage input titer) × 100. ( B ) Phage ELISAs performed on MERS-S2P-, SARS-CoV spike protein-, a CoV spike protein-immobilized surfaces (blue, red, and green, respectively). ( C ) Amino acid sequences of three unique clones identified from panning (left) and their relative frequencies (%) (right). The sequences were aligned using the Kabat numbering system [ 38 ]. ELISA, enzyme-linked immunosorbent assay; MERS-S2P, Middle East respiratory syndrome-CoV S2 subunit protein; SARS-SP, severe acute respiratory syndrome-CoV S protein; HKU1-SP, hCoV HKU1 S protein; CoV, coronavirus; CDR, complementarity-determining region; FR, framework region.

    Techniques Used: Clone Assay, Enzyme-linked Immunosorbent Assay

    Detection of MERS-S2P using S2A3 (IgG) on ACCEL ELISA™ plates. ( A ) Schematic depicting the sandwich ELISA format to detect MERS-S2P using S2A3 (IgG) (capture antibody) and rabbit anti-MERS-CoV IgG (detection antibody) on ACCEL ELISA™ plates. ( B ) ELISA detection of MERS-S2P on a capture antibody (S2A3 (IgG)) immobilized using three different concentrations (3 µg/mL, 5 µg/mL, and 10 µg/mL) on ACCEL ELISA™ plates. The goodness of fit is indicated by the R 2 value. LOD, limit of detection.
    Figure Legend Snippet: Detection of MERS-S2P using S2A3 (IgG) on ACCEL ELISA™ plates. ( A ) Schematic depicting the sandwich ELISA format to detect MERS-S2P using S2A3 (IgG) (capture antibody) and rabbit anti-MERS-CoV IgG (detection antibody) on ACCEL ELISA™ plates. ( B ) ELISA detection of MERS-S2P on a capture antibody (S2A3 (IgG)) immobilized using three different concentrations (3 µg/mL, 5 µg/mL, and 10 µg/mL) on ACCEL ELISA™ plates. The goodness of fit is indicated by the R 2 value. LOD, limit of detection.

    Techniques Used: Enzyme-linked Immunosorbent Assay, Sandwich ELISA

    2) Product Images from "Antiviral activity against Middle East Respiratory Syndrome coronavirus by Montelukast, an anti-asthma drug"

    Article Title: Antiviral activity against Middle East Respiratory Syndrome coronavirus by Montelukast, an anti-asthma drug

    Journal: Antiviral Research

    doi: 10.1016/j.antiviral.2020.104996

    MSH binds to MERS-CoV RBD in a dose-dependent manner. (A) Trp fluorescence quenching results of RBD and MSH, showing the extent of dose-dependent quenching from the comparison of peak heights and red-shift. Recombinant MERS-CoV RBD was used as control. (B) Binding curve plotted from quenching data of two independent assays. (C) RBD-MSH interaction analysis by Differential Scanning Fluorimetry (DSF). (D) Fluorescence quenching data on RBD mutants (V555A, R542D, Y540A) comparing the extent of quenching effect with MSH addition.
    Figure Legend Snippet: MSH binds to MERS-CoV RBD in a dose-dependent manner. (A) Trp fluorescence quenching results of RBD and MSH, showing the extent of dose-dependent quenching from the comparison of peak heights and red-shift. Recombinant MERS-CoV RBD was used as control. (B) Binding curve plotted from quenching data of two independent assays. (C) RBD-MSH interaction analysis by Differential Scanning Fluorimetry (DSF). (D) Fluorescence quenching data on RBD mutants (V555A, R542D, Y540A) comparing the extent of quenching effect with MSH addition.

    Techniques Used: Fluorescence, Recombinant, Binding Assay

    Inhibitory effect of MSH on MERS-CoV infection in Vero cells. Dose-response curves and immunofluorescence images of MSH addition compared to Chloroquine diphosphate (CQ) and Lopinavir (LPV) in two different conditions: (A) Pre-incubation, where MSH was mixed with MERS-CoV 1h before infection, and (B) Co-treatment, where MSH was mixed with MERS-CoV and transferred to the cells immediately. In both set-ups, viral inoculums and MSH treatment were incubated with the cells 24 h before fixation and harvesting. Points and blue lines represent the percentage of inhibition of MERS-CoV infection, and those in red refer to cell viability percentages. In the immunofluorescence images, red signals reflect viable cells, and green signals reflect viral progression.
    Figure Legend Snippet: Inhibitory effect of MSH on MERS-CoV infection in Vero cells. Dose-response curves and immunofluorescence images of MSH addition compared to Chloroquine diphosphate (CQ) and Lopinavir (LPV) in two different conditions: (A) Pre-incubation, where MSH was mixed with MERS-CoV 1h before infection, and (B) Co-treatment, where MSH was mixed with MERS-CoV and transferred to the cells immediately. In both set-ups, viral inoculums and MSH treatment were incubated with the cells 24 h before fixation and harvesting. Points and blue lines represent the percentage of inhibition of MERS-CoV infection, and those in red refer to cell viability percentages. In the immunofluorescence images, red signals reflect viable cells, and green signals reflect viral progression.

    Techniques Used: Infection, Immunofluorescence, Incubation, Inhibition

    Potential effects on MERS-CoV RBD-DPP4 complex formation inhibition and complex dissociation by MSH. Analytical gel filtration chromatography experiments exhibited slight but distinct and consistent peak shifts from RBD-DPP4 complex peak towards DPP4 only peak in (A) RBD + MSH followed by the addition of DPP4 and (B) RBD-DPP4 complex + MSH.
    Figure Legend Snippet: Potential effects on MERS-CoV RBD-DPP4 complex formation inhibition and complex dissociation by MSH. Analytical gel filtration chromatography experiments exhibited slight but distinct and consistent peak shifts from RBD-DPP4 complex peak towards DPP4 only peak in (A) RBD + MSH followed by the addition of DPP4 and (B) RBD-DPP4 complex + MSH.

    Techniques Used: Inhibition, Filtration, Chromatography

    In silico screening on the molecular interaction model of MSH with MERS-CoV RBD. (A) Chemical structure of MSH. (B) Cartoon model highlighting molecular interactions of MSH. The salt bridge interactions (black dotted lines) between the acetic acid group of MSH and R542 residue of MERS-CoV RBD. The ligand-binding was further stabilized by (i) π- π stacking interactions (highlighted in orange lines) between the phenyl, chloroquinoline groups of MSH with W553, Y540 residues respectively, (ii) alkyl hydrophobic interactions with surrounding V555, CH 2 sidechain atoms of R542, K502, and S557 residues of MERS-CoV RBD. (C) Potential surface view of MSH binding position on MERS-CoV RBD.
    Figure Legend Snippet: In silico screening on the molecular interaction model of MSH with MERS-CoV RBD. (A) Chemical structure of MSH. (B) Cartoon model highlighting molecular interactions of MSH. The salt bridge interactions (black dotted lines) between the acetic acid group of MSH and R542 residue of MERS-CoV RBD. The ligand-binding was further stabilized by (i) π- π stacking interactions (highlighted in orange lines) between the phenyl, chloroquinoline groups of MSH with W553, Y540 residues respectively, (ii) alkyl hydrophobic interactions with surrounding V555, CH 2 sidechain atoms of R542, K502, and S557 residues of MERS-CoV RBD. (C) Potential surface view of MSH binding position on MERS-CoV RBD.

    Techniques Used: In Silico, Ligand Binding Assay, Binding Assay

    3) Product Images from "Safety and immunogenicity of a candidate Middle East respiratory syndrome coronavirus viral-vectored vaccine: a dose-escalation, open-label, non-randomised, uncontrolled, phase 1 trial"

    Article Title: Safety and immunogenicity of a candidate Middle East respiratory syndrome coronavirus viral-vectored vaccine: a dose-escalation, open-label, non-randomised, uncontrolled, phase 1 trial

    Journal: The Lancet. Infectious Diseases

    doi: 10.1016/S1473-3099(20)30160-2

    MERS-CoV spike-pseudotyped neutralisation p values were calculated using Kruskall-Wallis with Dunn's multiple comparison post test. The dashed lines represent lower limit of detection under our experimental condition. Data points represent geometric means, and error bars represent 95% CIs. MERS-CoV=Middle East respiratory syndrome coronavirus.
    Figure Legend Snippet: MERS-CoV spike-pseudotyped neutralisation p values were calculated using Kruskall-Wallis with Dunn's multiple comparison post test. The dashed lines represent lower limit of detection under our experimental condition. Data points represent geometric means, and error bars represent 95% CIs. MERS-CoV=Middle East respiratory syndrome coronavirus.

    Techniques Used:

    Related Articles

    Positive Control:

    Article Title: Safety and immunogenicity of a candidate Middle East respiratory syndrome coronavirus viral-vectored vaccine: a dose-escalation, open-label, non-randomised, uncontrolled, phase 1 trial
    Article Snippet: Two-fold diluted serum was incubated with 2 × 106 viral particles of pseudotyped virus for 60 min at 37°C and added to 786-O cells, incubated for 6 h, and replaced with fresh medium; luciferase-reporter activity was measured 3 days later. .. A commercially available neutralising monoclonal antibody (40069-R723, Sino Biological, Beijing, China) was used as a positive control. .. To assess cellular immunity, interferon-γ-linked enzyme-linked immunospot (ELISpot) assays were done with fresh peripheral blood mononuclear cells (PBMCs) to determine responses to the MERS-CoV spike vaccine antigen.

    Infection:

    Article Title: Antiviral activity against Middle East Respiratory Syndrome coronavirus by Montelukast, an anti-asthma drug
    Article Snippet: 2.11 Immunofluorescent staining The viral inoculums and drug treatments were incubated with the cells for 24 h. After this, the infected cells were fixed at 24 h post-infection (hpi) with 4% PFA and permeabilized with 0.25% Triton X-100 (Sigma Aldrich, USA) in Phosphate Buffer Saline (PBS) for 30 min. .. Infected cells were visualized by immunofluorescence of viral spike protein, which was detected by rabbit anti-MERS-CoV spike antibodies (Sino-Biological, CN), and cell viability was evaluated with Hoechst 33,342 (Thermo Fisher Scientific) stain. .. The immunofluorescence images were acquired using PerkinElmer Operetta (20x, Waltham, MA) and analyzed by the in-house Image Mining 3.0 (IM 3.0) software to quantify cell numbers and infection ratios.

    Article Title: Recombinant influenza H7 hemagglutinin containing CFLLC minidomain in the transmembrane domain showed enhanced cross-protection in mice.
    Article Snippet: Since February 2013, H7N9 influenza virus, causing human infections with high mortality in China, has been a potential pandemic threat. .. Since February 2013, H7N9 influenza virus, causing human infections with high mortality in China, has been a potential pandemic threat. .. Since February 2013, H7N9 influenza virus, causing human infections with high mortality in China, has been a potential pandemic threat.

    Article Title: CD8+ T Cells Responding to the Middle East Respiratory Syndrome Coronavirus Nucleocapsid Protein Delivered by Vaccinia Virus MVA in Mice
    Article Snippet: Total cell proteins were resolved by electrophoresis in a sodium dodecyl sulfate (SDS)-10% polyacrylamide gel (SDS-PAGE) and subsequently transferred onto a nitrocellulose membrane via electroblotting. .. After 1 h blocking in a phosphate buffered saline (PBS) buffer containing 1% (w/v) non-fat dried milk and 0.1% (v/v) NP-40 detergent, the blots were incubated with monoclonal mouse anti-MERS-CoV Nucleocapsid antibody (Sino Biological, Beijing, China, 1:1000), monoclonal rabbit anti-MERS-CoV Spike Protein S1 Antibody (Sino Biological, 1:500), or polyclonal sera from MERS-CoV infected rabbits or cynomolgus macaques (kindly provided by Dr. Bart Haagmans, Erasmus Medical Center, Rotterdam, 1:1000) [ ] as primary antibodies. .. After washing with 0.1% NP-40 in PBS, the blots were incubated with anti-mouse IgG (1:5000), or anti-rabbit IgG antibody (1:5000), or protein A (1:1000) conjugated to horseradish peroxidase (Cell Signaling Technology, Frankfurt am Main, Germany).

    Article Title: “A recombinant protein SARS-CoV-2 candidate vaccine elicits high-titer neutralizing antibodies in macaques”
    Article Snippet: Nanoluc Luciferase activity in lysates was measured using the Nano-Glo Luciferase Assay System (Promega) with the Modulus II Microplate Reader (Turner BioSystems). .. The raw nanoluc luciferase activity values (relative luminescence units) were normalized to those derived from cells infected with SARS-CoV-2 pseudotyped virus in the absence of serum or a rabbit monoclonal antibody diluted in normal human serum at 0.105 mg/mL (40592-R001, Sinobiological, Wayne, PA). .. The half-maximal inhibitory concentration for serum (NT50) was determined using four-parameter nonlinear regression (GraphPad Prism).

    Immunofluorescence:

    Article Title: Antiviral activity against Middle East Respiratory Syndrome coronavirus by Montelukast, an anti-asthma drug
    Article Snippet: 2.11 Immunofluorescent staining The viral inoculums and drug treatments were incubated with the cells for 24 h. After this, the infected cells were fixed at 24 h post-infection (hpi) with 4% PFA and permeabilized with 0.25% Triton X-100 (Sigma Aldrich, USA) in Phosphate Buffer Saline (PBS) for 30 min. .. Infected cells were visualized by immunofluorescence of viral spike protein, which was detected by rabbit anti-MERS-CoV spike antibodies (Sino-Biological, CN), and cell viability was evaluated with Hoechst 33,342 (Thermo Fisher Scientific) stain. .. The immunofluorescence images were acquired using PerkinElmer Operetta (20x, Waltham, MA) and analyzed by the in-house Image Mining 3.0 (IM 3.0) software to quantify cell numbers and infection ratios.

    Staining:

    Article Title: Antiviral activity against Middle East Respiratory Syndrome coronavirus by Montelukast, an anti-asthma drug
    Article Snippet: 2.11 Immunofluorescent staining The viral inoculums and drug treatments were incubated with the cells for 24 h. After this, the infected cells were fixed at 24 h post-infection (hpi) with 4% PFA and permeabilized with 0.25% Triton X-100 (Sigma Aldrich, USA) in Phosphate Buffer Saline (PBS) for 30 min. .. Infected cells were visualized by immunofluorescence of viral spike protein, which was detected by rabbit anti-MERS-CoV spike antibodies (Sino-Biological, CN), and cell viability was evaluated with Hoechst 33,342 (Thermo Fisher Scientific) stain. .. The immunofluorescence images were acquired using PerkinElmer Operetta (20x, Waltham, MA) and analyzed by the in-house Image Mining 3.0 (IM 3.0) software to quantify cell numbers and infection ratios.

    Article Title: Diffuse High Intensity PD-L1 Staining in Thymic Epithelial Tumors
    Article Snippet: .. The TMA was stained with rabbit monoclonal antibody (clone 15, Sino Biological) to human PD-L1. .. PD-L1 staining was scored based on intensity as follows: 0=none, 1=equivocal/uninterpretable, 2=weak, and 3=intermediate-strong.

    Incubation:

    Article Title: Selection and Characterization of Monoclonal Antibodies Targeting Middle East Respiratory Syndrome Coronavirus through a Human Synthetic Fab Phage Display Library Panning
    Article Snippet: .. The rabbit anti-MERS-CoV antibodies (1:3000, Sino Biological), serially two-fold-diluted MERS-S2P, and HRP-conjugated goat anti-rabbit antibodies (1:6000, Sigma Aldrich) were added and incubated for 30 min at RT. ..

    Article Title: CD8+ T Cells Responding to the Middle East Respiratory Syndrome Coronavirus Nucleocapsid Protein Delivered by Vaccinia Virus MVA in Mice
    Article Snippet: Total cell proteins were resolved by electrophoresis in a sodium dodecyl sulfate (SDS)-10% polyacrylamide gel (SDS-PAGE) and subsequently transferred onto a nitrocellulose membrane via electroblotting. .. After 1 h blocking in a phosphate buffered saline (PBS) buffer containing 1% (w/v) non-fat dried milk and 0.1% (v/v) NP-40 detergent, the blots were incubated with monoclonal mouse anti-MERS-CoV Nucleocapsid antibody (Sino Biological, Beijing, China, 1:1000), monoclonal rabbit anti-MERS-CoV Spike Protein S1 Antibody (Sino Biological, 1:500), or polyclonal sera from MERS-CoV infected rabbits or cynomolgus macaques (kindly provided by Dr. Bart Haagmans, Erasmus Medical Center, Rotterdam, 1:1000) [ ] as primary antibodies. .. After washing with 0.1% NP-40 in PBS, the blots were incubated with anti-mouse IgG (1:5000), or anti-rabbit IgG antibody (1:5000), or protein A (1:1000) conjugated to horseradish peroxidase (Cell Signaling Technology, Frankfurt am Main, Germany).

    Article Title: Recombinant influenza H7 hemagglutinin containing CFLLC minidomain in the transmembrane domain showed enhanced cross-protection in mice.
    Article Snippet: Since February 2013, H7N9 influenza virus, causing human infections with high mortality in China, has been a potential pandemic threat. .. Since February 2013, H7N9 influenza virus, causing human infections with high mortality in China, has been a potential pandemic threat. .. Since February 2013, H7N9 influenza virus, causing human infections with high mortality in China, has been a potential pandemic threat.

    Blocking Assay:

    Article Title: CD8+ T Cells Responding to the Middle East Respiratory Syndrome Coronavirus Nucleocapsid Protein Delivered by Vaccinia Virus MVA in Mice
    Article Snippet: Total cell proteins were resolved by electrophoresis in a sodium dodecyl sulfate (SDS)-10% polyacrylamide gel (SDS-PAGE) and subsequently transferred onto a nitrocellulose membrane via electroblotting. .. After 1 h blocking in a phosphate buffered saline (PBS) buffer containing 1% (w/v) non-fat dried milk and 0.1% (v/v) NP-40 detergent, the blots were incubated with monoclonal mouse anti-MERS-CoV Nucleocapsid antibody (Sino Biological, Beijing, China, 1:1000), monoclonal rabbit anti-MERS-CoV Spike Protein S1 Antibody (Sino Biological, 1:500), or polyclonal sera from MERS-CoV infected rabbits or cynomolgus macaques (kindly provided by Dr. Bart Haagmans, Erasmus Medical Center, Rotterdam, 1:1000) [ ] as primary antibodies. .. After washing with 0.1% NP-40 in PBS, the blots were incubated with anti-mouse IgG (1:5000), or anti-rabbit IgG antibody (1:5000), or protein A (1:1000) conjugated to horseradish peroxidase (Cell Signaling Technology, Frankfurt am Main, Germany).

    Luciferase:

    Article Title: “A recombinant protein SARS-CoV-2 candidate vaccine elicits high-titer neutralizing antibodies in macaques”
    Article Snippet: Nanoluc Luciferase activity in lysates was measured using the Nano-Glo Luciferase Assay System (Promega) with the Modulus II Microplate Reader (Turner BioSystems). .. The raw nanoluc luciferase activity values (relative luminescence units) were normalized to those derived from cells infected with SARS-CoV-2 pseudotyped virus in the absence of serum or a rabbit monoclonal antibody diluted in normal human serum at 0.105 mg/mL (40592-R001, Sinobiological, Wayne, PA). .. The half-maximal inhibitory concentration for serum (NT50) was determined using four-parameter nonlinear regression (GraphPad Prism).

    Activity Assay:

    Article Title: “A recombinant protein SARS-CoV-2 candidate vaccine elicits high-titer neutralizing antibodies in macaques”
    Article Snippet: Nanoluc Luciferase activity in lysates was measured using the Nano-Glo Luciferase Assay System (Promega) with the Modulus II Microplate Reader (Turner BioSystems). .. The raw nanoluc luciferase activity values (relative luminescence units) were normalized to those derived from cells infected with SARS-CoV-2 pseudotyped virus in the absence of serum or a rabbit monoclonal antibody diluted in normal human serum at 0.105 mg/mL (40592-R001, Sinobiological, Wayne, PA). .. The half-maximal inhibitory concentration for serum (NT50) was determined using four-parameter nonlinear regression (GraphPad Prism).

    Derivative Assay:

    Article Title: “A recombinant protein SARS-CoV-2 candidate vaccine elicits high-titer neutralizing antibodies in macaques”
    Article Snippet: Nanoluc Luciferase activity in lysates was measured using the Nano-Glo Luciferase Assay System (Promega) with the Modulus II Microplate Reader (Turner BioSystems). .. The raw nanoluc luciferase activity values (relative luminescence units) were normalized to those derived from cells infected with SARS-CoV-2 pseudotyped virus in the absence of serum or a rabbit monoclonal antibody diluted in normal human serum at 0.105 mg/mL (40592-R001, Sinobiological, Wayne, PA). .. The half-maximal inhibitory concentration for serum (NT50) was determined using four-parameter nonlinear regression (GraphPad Prism).

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  • 95
    Sino Biological rabbit anti mers cov antibodies
    Characterization of <t>anti-MERS-S2P</t> IgGs. ( A ) Immunofluorescence assay of anti-MERS-S2P IgGs on cell lines infected with various human coronaviruses (hCoVs) to determine their <t>MERS-CoV</t> specificity. Scale bar, 200 μm. ( B ) Size-exclusion chromatography analysis of S2A3 (IgG) and S2D5 (IgG). The molecular weights (kDa) of the molecular mass markers are shown above the corresponding peaks at the top chromatogram. ( C ) Surface plasmon resonance (SPR) analysis of S2A3 (IgG) and S2D5 (IgG) on a MERS-S2P-immobilized sensor chip to determine their apparent binding affinities. The fitted-lines are marked by red.
    Rabbit Anti Mers Cov Antibodies, supplied by Sino Biological, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti mers cov antibodies/product/Sino Biological
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti mers cov antibodies - by Bioz Stars, 2021-04
    95/100 stars
      Buy from Supplier

    Image Search Results


    Characterization of anti-MERS-S2P IgGs. ( A ) Immunofluorescence assay of anti-MERS-S2P IgGs on cell lines infected with various human coronaviruses (hCoVs) to determine their MERS-CoV specificity. Scale bar, 200 μm. ( B ) Size-exclusion chromatography analysis of S2A3 (IgG) and S2D5 (IgG). The molecular weights (kDa) of the molecular mass markers are shown above the corresponding peaks at the top chromatogram. ( C ) Surface plasmon resonance (SPR) analysis of S2A3 (IgG) and S2D5 (IgG) on a MERS-S2P-immobilized sensor chip to determine their apparent binding affinities. The fitted-lines are marked by red.

    Journal: Antibodies

    Article Title: Selection and Characterization of Monoclonal Antibodies Targeting Middle East Respiratory Syndrome Coronavirus through a Human Synthetic Fab Phage Display Library Panning

    doi: 10.3390/antib8030042

    Figure Lengend Snippet: Characterization of anti-MERS-S2P IgGs. ( A ) Immunofluorescence assay of anti-MERS-S2P IgGs on cell lines infected with various human coronaviruses (hCoVs) to determine their MERS-CoV specificity. Scale bar, 200 μm. ( B ) Size-exclusion chromatography analysis of S2A3 (IgG) and S2D5 (IgG). The molecular weights (kDa) of the molecular mass markers are shown above the corresponding peaks at the top chromatogram. ( C ) Surface plasmon resonance (SPR) analysis of S2A3 (IgG) and S2D5 (IgG) on a MERS-S2P-immobilized sensor chip to determine their apparent binding affinities. The fitted-lines are marked by red.

    Article Snippet: The rabbit anti-MERS-CoV antibodies (1:3000, Sino Biological), serially two-fold-diluted MERS-S2P, and HRP-conjugated goat anti-rabbit antibodies (1:6000, Sigma Aldrich) were added and incubated for 30 min at RT.

    Techniques: Immunofluorescence, Infection, Size-exclusion Chromatography, SPR Assay, Chromatin Immunoprecipitation, Binding Assay

    Output of the panning of the phage-displayed synthetic Fab library on MERS-S2P. ( A ) Monitoring of phage titers over three rounds (R1–R3) of panning. Black and gray bars indicate a ratio of phage output to input titers presented as a percentage (%) from panning on MERS-S2P-immobilized and -non-immobilized surfaces, respectively. The ratio of output to input (%) = (phage output titer ÷ phage input titer) × 100. ( B ) Phage ELISAs performed on MERS-S2P-, SARS-CoV spike protein-, a CoV spike protein-immobilized surfaces (blue, red, and green, respectively). ( C ) Amino acid sequences of three unique clones identified from panning (left) and their relative frequencies (%) (right). The sequences were aligned using the Kabat numbering system [ 38 ]. ELISA, enzyme-linked immunosorbent assay; MERS-S2P, Middle East respiratory syndrome-CoV S2 subunit protein; SARS-SP, severe acute respiratory syndrome-CoV S protein; HKU1-SP, hCoV HKU1 S protein; CoV, coronavirus; CDR, complementarity-determining region; FR, framework region.

    Journal: Antibodies

    Article Title: Selection and Characterization of Monoclonal Antibodies Targeting Middle East Respiratory Syndrome Coronavirus through a Human Synthetic Fab Phage Display Library Panning

    doi: 10.3390/antib8030042

    Figure Lengend Snippet: Output of the panning of the phage-displayed synthetic Fab library on MERS-S2P. ( A ) Monitoring of phage titers over three rounds (R1–R3) of panning. Black and gray bars indicate a ratio of phage output to input titers presented as a percentage (%) from panning on MERS-S2P-immobilized and -non-immobilized surfaces, respectively. The ratio of output to input (%) = (phage output titer ÷ phage input titer) × 100. ( B ) Phage ELISAs performed on MERS-S2P-, SARS-CoV spike protein-, a CoV spike protein-immobilized surfaces (blue, red, and green, respectively). ( C ) Amino acid sequences of three unique clones identified from panning (left) and their relative frequencies (%) (right). The sequences were aligned using the Kabat numbering system [ 38 ]. ELISA, enzyme-linked immunosorbent assay; MERS-S2P, Middle East respiratory syndrome-CoV S2 subunit protein; SARS-SP, severe acute respiratory syndrome-CoV S protein; HKU1-SP, hCoV HKU1 S protein; CoV, coronavirus; CDR, complementarity-determining region; FR, framework region.

    Article Snippet: The rabbit anti-MERS-CoV antibodies (1:3000, Sino Biological), serially two-fold-diluted MERS-S2P, and HRP-conjugated goat anti-rabbit antibodies (1:6000, Sigma Aldrich) were added and incubated for 30 min at RT.

    Techniques: Clone Assay, Enzyme-linked Immunosorbent Assay

    Detection of MERS-S2P using S2A3 (IgG) on ACCEL ELISA™ plates. ( A ) Schematic depicting the sandwich ELISA format to detect MERS-S2P using S2A3 (IgG) (capture antibody) and rabbit anti-MERS-CoV IgG (detection antibody) on ACCEL ELISA™ plates. ( B ) ELISA detection of MERS-S2P on a capture antibody (S2A3 (IgG)) immobilized using three different concentrations (3 µg/mL, 5 µg/mL, and 10 µg/mL) on ACCEL ELISA™ plates. The goodness of fit is indicated by the R 2 value. LOD, limit of detection.

    Journal: Antibodies

    Article Title: Selection and Characterization of Monoclonal Antibodies Targeting Middle East Respiratory Syndrome Coronavirus through a Human Synthetic Fab Phage Display Library Panning

    doi: 10.3390/antib8030042

    Figure Lengend Snippet: Detection of MERS-S2P using S2A3 (IgG) on ACCEL ELISA™ plates. ( A ) Schematic depicting the sandwich ELISA format to detect MERS-S2P using S2A3 (IgG) (capture antibody) and rabbit anti-MERS-CoV IgG (detection antibody) on ACCEL ELISA™ plates. ( B ) ELISA detection of MERS-S2P on a capture antibody (S2A3 (IgG)) immobilized using three different concentrations (3 µg/mL, 5 µg/mL, and 10 µg/mL) on ACCEL ELISA™ plates. The goodness of fit is indicated by the R 2 value. LOD, limit of detection.

    Article Snippet: The rabbit anti-MERS-CoV antibodies (1:3000, Sino Biological), serially two-fold-diluted MERS-S2P, and HRP-conjugated goat anti-rabbit antibodies (1:6000, Sigma Aldrich) were added and incubated for 30 min at RT.

    Techniques: Enzyme-linked Immunosorbent Assay, Sandwich ELISA

    MSH binds to MERS-CoV RBD in a dose-dependent manner. (A) Trp fluorescence quenching results of RBD and MSH, showing the extent of dose-dependent quenching from the comparison of peak heights and red-shift. Recombinant MERS-CoV RBD was used as control. (B) Binding curve plotted from quenching data of two independent assays. (C) RBD-MSH interaction analysis by Differential Scanning Fluorimetry (DSF). (D) Fluorescence quenching data on RBD mutants (V555A, R542D, Y540A) comparing the extent of quenching effect with MSH addition.

    Journal: Antiviral Research

    Article Title: Antiviral activity against Middle East Respiratory Syndrome coronavirus by Montelukast, an anti-asthma drug

    doi: 10.1016/j.antiviral.2020.104996

    Figure Lengend Snippet: MSH binds to MERS-CoV RBD in a dose-dependent manner. (A) Trp fluorescence quenching results of RBD and MSH, showing the extent of dose-dependent quenching from the comparison of peak heights and red-shift. Recombinant MERS-CoV RBD was used as control. (B) Binding curve plotted from quenching data of two independent assays. (C) RBD-MSH interaction analysis by Differential Scanning Fluorimetry (DSF). (D) Fluorescence quenching data on RBD mutants (V555A, R542D, Y540A) comparing the extent of quenching effect with MSH addition.

    Article Snippet: Infected cells were visualized by immunofluorescence of viral spike protein, which was detected by rabbit anti-MERS-CoV spike antibodies (Sino-Biological, CN), and cell viability was evaluated with Hoechst 33,342 (Thermo Fisher Scientific) stain.

    Techniques: Fluorescence, Recombinant, Binding Assay

    Inhibitory effect of MSH on MERS-CoV infection in Vero cells. Dose-response curves and immunofluorescence images of MSH addition compared to Chloroquine diphosphate (CQ) and Lopinavir (LPV) in two different conditions: (A) Pre-incubation, where MSH was mixed with MERS-CoV 1h before infection, and (B) Co-treatment, where MSH was mixed with MERS-CoV and transferred to the cells immediately. In both set-ups, viral inoculums and MSH treatment were incubated with the cells 24 h before fixation and harvesting. Points and blue lines represent the percentage of inhibition of MERS-CoV infection, and those in red refer to cell viability percentages. In the immunofluorescence images, red signals reflect viable cells, and green signals reflect viral progression.

    Journal: Antiviral Research

    Article Title: Antiviral activity against Middle East Respiratory Syndrome coronavirus by Montelukast, an anti-asthma drug

    doi: 10.1016/j.antiviral.2020.104996

    Figure Lengend Snippet: Inhibitory effect of MSH on MERS-CoV infection in Vero cells. Dose-response curves and immunofluorescence images of MSH addition compared to Chloroquine diphosphate (CQ) and Lopinavir (LPV) in two different conditions: (A) Pre-incubation, where MSH was mixed with MERS-CoV 1h before infection, and (B) Co-treatment, where MSH was mixed with MERS-CoV and transferred to the cells immediately. In both set-ups, viral inoculums and MSH treatment were incubated with the cells 24 h before fixation and harvesting. Points and blue lines represent the percentage of inhibition of MERS-CoV infection, and those in red refer to cell viability percentages. In the immunofluorescence images, red signals reflect viable cells, and green signals reflect viral progression.

    Article Snippet: Infected cells were visualized by immunofluorescence of viral spike protein, which was detected by rabbit anti-MERS-CoV spike antibodies (Sino-Biological, CN), and cell viability was evaluated with Hoechst 33,342 (Thermo Fisher Scientific) stain.

    Techniques: Infection, Immunofluorescence, Incubation, Inhibition

    Potential effects on MERS-CoV RBD-DPP4 complex formation inhibition and complex dissociation by MSH. Analytical gel filtration chromatography experiments exhibited slight but distinct and consistent peak shifts from RBD-DPP4 complex peak towards DPP4 only peak in (A) RBD + MSH followed by the addition of DPP4 and (B) RBD-DPP4 complex + MSH.

    Journal: Antiviral Research

    Article Title: Antiviral activity against Middle East Respiratory Syndrome coronavirus by Montelukast, an anti-asthma drug

    doi: 10.1016/j.antiviral.2020.104996

    Figure Lengend Snippet: Potential effects on MERS-CoV RBD-DPP4 complex formation inhibition and complex dissociation by MSH. Analytical gel filtration chromatography experiments exhibited slight but distinct and consistent peak shifts from RBD-DPP4 complex peak towards DPP4 only peak in (A) RBD + MSH followed by the addition of DPP4 and (B) RBD-DPP4 complex + MSH.

    Article Snippet: Infected cells were visualized by immunofluorescence of viral spike protein, which was detected by rabbit anti-MERS-CoV spike antibodies (Sino-Biological, CN), and cell viability was evaluated with Hoechst 33,342 (Thermo Fisher Scientific) stain.

    Techniques: Inhibition, Filtration, Chromatography

    In silico screening on the molecular interaction model of MSH with MERS-CoV RBD. (A) Chemical structure of MSH. (B) Cartoon model highlighting molecular interactions of MSH. The salt bridge interactions (black dotted lines) between the acetic acid group of MSH and R542 residue of MERS-CoV RBD. The ligand-binding was further stabilized by (i) π- π stacking interactions (highlighted in orange lines) between the phenyl, chloroquinoline groups of MSH with W553, Y540 residues respectively, (ii) alkyl hydrophobic interactions with surrounding V555, CH 2 sidechain atoms of R542, K502, and S557 residues of MERS-CoV RBD. (C) Potential surface view of MSH binding position on MERS-CoV RBD.

    Journal: Antiviral Research

    Article Title: Antiviral activity against Middle East Respiratory Syndrome coronavirus by Montelukast, an anti-asthma drug

    doi: 10.1016/j.antiviral.2020.104996

    Figure Lengend Snippet: In silico screening on the molecular interaction model of MSH with MERS-CoV RBD. (A) Chemical structure of MSH. (B) Cartoon model highlighting molecular interactions of MSH. The salt bridge interactions (black dotted lines) between the acetic acid group of MSH and R542 residue of MERS-CoV RBD. The ligand-binding was further stabilized by (i) π- π stacking interactions (highlighted in orange lines) between the phenyl, chloroquinoline groups of MSH with W553, Y540 residues respectively, (ii) alkyl hydrophobic interactions with surrounding V555, CH 2 sidechain atoms of R542, K502, and S557 residues of MERS-CoV RBD. (C) Potential surface view of MSH binding position on MERS-CoV RBD.

    Article Snippet: Infected cells were visualized by immunofluorescence of viral spike protein, which was detected by rabbit anti-MERS-CoV spike antibodies (Sino-Biological, CN), and cell viability was evaluated with Hoechst 33,342 (Thermo Fisher Scientific) stain.

    Techniques: In Silico, Ligand Binding Assay, Binding Assay

    MERS-CoV spike-pseudotyped neutralisation p values were calculated using Kruskall-Wallis with Dunn's multiple comparison post test. The dashed lines represent lower limit of detection under our experimental condition. Data points represent geometric means, and error bars represent 95% CIs. MERS-CoV=Middle East respiratory syndrome coronavirus.

    Journal: The Lancet. Infectious Diseases

    Article Title: Safety and immunogenicity of a candidate Middle East respiratory syndrome coronavirus viral-vectored vaccine: a dose-escalation, open-label, non-randomised, uncontrolled, phase 1 trial

    doi: 10.1016/S1473-3099(20)30160-2

    Figure Lengend Snippet: MERS-CoV spike-pseudotyped neutralisation p values were calculated using Kruskall-Wallis with Dunn's multiple comparison post test. The dashed lines represent lower limit of detection under our experimental condition. Data points represent geometric means, and error bars represent 95% CIs. MERS-CoV=Middle East respiratory syndrome coronavirus.

    Article Snippet: A commercially available neutralising monoclonal antibody (40069-R723, Sino Biological, Beijing, China) was used as a positive control.

    Techniques: