hku1 s1 protein  (Sino Biological)


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    Name:
    Human coronavirus HKU1 Spike S1 Protein
    Description:
    A DNA sequence encoding the human coronavirus HKU1 isolate N1 HCoV HKU1 spike protein S1 Subunit YP 173238 1 Met 1 Arg 760 was expressed with a polyhistidine tag at the C terminus
    Catalog Number:
    40021-V08H
    Price:
    None
    Category:
    recombinant protein
    Host:
    HEK293 Cells
    Buy from Supplier


    Structured Review

    Sino Biological hku1 s1 protein
    A DNA sequence encoding the human coronavirus HKU1 isolate N1 HCoV HKU1 spike protein S1 Subunit YP 173238 1 Met 1 Arg 760 was expressed with a polyhistidine tag at the C terminus
    https://www.bioz.com/result/hku1 s1 protein/product/Sino Biological
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hku1 s1 protein - by Bioz Stars, 2021-06
    95/100 stars

    Images

    Related Articles

    Incubation:

    Article Title: Intranasal administration of SARS-CoV-2 neutralizing human antibody prevents infection in mice
    Article Snippet: For protein panning, 5 mg/mL SARS-CoV-2 spike protein (Sino biological, 40591-V08H) was coated on high binding plates (Corning, #3361). .. Human scFv phage libraries were incubated with a mixture of 6 other coronaviruses for negative selection: SARS-CoV (Sino biological, 40150-V08B1), MERS-CoV (Sino biological, 40069-V08H), HCoV-HKU1 (Sino biological, 40021-V08H), HCoV-NL63 (Sino biological, 40600-V08H), HCoV-229E (Sino biological, 40601-V08H), HCoV-OC43 (Sino biological, 40607-V08B), before adding to SARS-CoV-2 spike protein coated plates. .. After extended washing with PBS, the bound clones were eluted and used to infect E.coli XL1-Blue cells.

    Article Title: Immune Responses to SARS-CoV-2 Infection in Hospitalized Pediatric and Adult Patients
    Article Snippet: .. Recombinant SARS Cov-2 spike protein (ACROBiosystems #S1N-C52H3), HKU1 S1 protein (Sino Biological #40021-V08H-100), 229E S1 protein (Sino Biological # 40601-V08H-100) or NL63 S1 protein (Sino Biological # 40600-V08H-100) at a concentration of 100 ng in 50μl PBS) were added to 96-well MaxiSorp plates (Thermo Scientific #442404) and incubated overnight at 4°C. .. Plates were washed three times with 250 μL ELISA wash buffer PBS-T (PBS with 0.1% Tween-20) and incubated with 200 μl of blocking solution (PBS with 0.1% Tween-20, 3% milk powder) at room temperature (RT) for 1.5 hours.

    Selection:

    Article Title: Intranasal administration of SARS-CoV-2 neutralizing human antibody prevents infection in mice
    Article Snippet: For protein panning, 5 mg/mL SARS-CoV-2 spike protein (Sino biological, 40591-V08H) was coated on high binding plates (Corning, #3361). .. Human scFv phage libraries were incubated with a mixture of 6 other coronaviruses for negative selection: SARS-CoV (Sino biological, 40150-V08B1), MERS-CoV (Sino biological, 40069-V08H), HCoV-HKU1 (Sino biological, 40021-V08H), HCoV-NL63 (Sino biological, 40600-V08H), HCoV-229E (Sino biological, 40601-V08H), HCoV-OC43 (Sino biological, 40607-V08B), before adding to SARS-CoV-2 spike protein coated plates. .. After extended washing with PBS, the bound clones were eluted and used to infect E.coli XL1-Blue cells.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Comparative Serological Study for the Prevalence of Anti-MERS Coronavirus Antibodies in High- and Low-Risk Groups in Qatar
    Article Snippet: Final confirmation was performed using pseudoparticle neutralization test (ppNT) against two MERS-CoV strains, the EMC strain (GenBank JX869059) and the Jordan N3 strain (GenBank KC776174), as previously described [ ]. .. The determination of cross-reactivity of borderline and reactive samples against other human coronaviruses was performed using: (i) commercially available whole-virus IgM/IgG IIFT for SARS-CoV (Euroimmun, cat no. FI 2601-1010 G/M), (ii) prototype whole-virus IgG IIFT kit for HCoV-229E (Euroimmun, prototype kit), (iii) in-house ELISA for HKU1-CoV using recombinant S1 protein (Sino Biological Inc., catalog # 40021-V08H), and (iv) IgG rS1-IIFT for all other human-CoV (Institute of Virology, Charité - Universitätsmedizin Berlin, Germany) [ ]. .. Initial screening for anti-MERS-CoV antibodies using IgG rS1-ELISA revealed 10/4719 (0.21%) and 1/135 (0.74%) reactive samples from BD (three borderline and seven positive samples) and CC, respectively.

    Recombinant:

    Article Title: Comparative Serological Study for the Prevalence of Anti-MERS Coronavirus Antibodies in High- and Low-Risk Groups in Qatar
    Article Snippet: Final confirmation was performed using pseudoparticle neutralization test (ppNT) against two MERS-CoV strains, the EMC strain (GenBank JX869059) and the Jordan N3 strain (GenBank KC776174), as previously described [ ]. .. The determination of cross-reactivity of borderline and reactive samples against other human coronaviruses was performed using: (i) commercially available whole-virus IgM/IgG IIFT for SARS-CoV (Euroimmun, cat no. FI 2601-1010 G/M), (ii) prototype whole-virus IgG IIFT kit for HCoV-229E (Euroimmun, prototype kit), (iii) in-house ELISA for HKU1-CoV using recombinant S1 protein (Sino Biological Inc., catalog # 40021-V08H), and (iv) IgG rS1-IIFT for all other human-CoV (Institute of Virology, Charité - Universitätsmedizin Berlin, Germany) [ ]. .. Initial screening for anti-MERS-CoV antibodies using IgG rS1-ELISA revealed 10/4719 (0.21%) and 1/135 (0.74%) reactive samples from BD (three borderline and seven positive samples) and CC, respectively.

    Article Title: Immune Responses to SARS-CoV-2 Infection in Hospitalized Pediatric and Adult Patients
    Article Snippet: .. Recombinant SARS Cov-2 spike protein (ACROBiosystems #S1N-C52H3), HKU1 S1 protein (Sino Biological #40021-V08H-100), 229E S1 protein (Sino Biological # 40601-V08H-100) or NL63 S1 protein (Sino Biological # 40600-V08H-100) at a concentration of 100 ng in 50μl PBS) were added to 96-well MaxiSorp plates (Thermo Scientific #442404) and incubated overnight at 4°C. .. Plates were washed three times with 250 μL ELISA wash buffer PBS-T (PBS with 0.1% Tween-20) and incubated with 200 μl of blocking solution (PBS with 0.1% Tween-20, 3% milk powder) at room temperature (RT) for 1.5 hours.

    Concentration Assay:

    Article Title: Immune Responses to SARS-CoV-2 Infection in Hospitalized Pediatric and Adult Patients
    Article Snippet: .. Recombinant SARS Cov-2 spike protein (ACROBiosystems #S1N-C52H3), HKU1 S1 protein (Sino Biological #40021-V08H-100), 229E S1 protein (Sino Biological # 40601-V08H-100) or NL63 S1 protein (Sino Biological # 40600-V08H-100) at a concentration of 100 ng in 50μl PBS) were added to 96-well MaxiSorp plates (Thermo Scientific #442404) and incubated overnight at 4°C. .. Plates were washed three times with 250 μL ELISA wash buffer PBS-T (PBS with 0.1% Tween-20) and incubated with 200 μl of blocking solution (PBS with 0.1% Tween-20, 3% milk powder) at room temperature (RT) for 1.5 hours.

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  • 93
    Sino Biological rabbit polyclonal anti hcov hku1 spike protein antibody
    Output of the panning of the phage-displayed synthetic Fab library on MERS-S2P. ( A ) Monitoring of phage titers over three rounds (R1–R3) of panning. Black and gray bars indicate a ratio of phage output to input titers presented as a percentage (%) from panning on MERS-S2P-immobilized and -non-immobilized surfaces, respectively. The ratio of output to input (%) = (phage output titer ÷ phage input titer) × 100. ( B ) Phage ELISAs performed on MERS-S2P-, SARS-CoV spike protein-, a CoV spike protein-immobilized surfaces (blue, red, and green, respectively). ( C ) Amino acid sequences of three unique clones identified from panning (left) and their relative frequencies (%) (right). The sequences were aligned using the Kabat numbering system [ 38 ]. ELISA, enzyme-linked immunosorbent assay; MERS-S2P, Middle East respiratory syndrome-CoV S2 subunit protein; SARS-SP, severe acute respiratory syndrome-CoV S protein; <t>HKU1-SP,</t> <t>hCoV</t> HKU1 S protein; CoV, coronavirus; CDR, complementarity-determining region; FR, framework region.
    Rabbit Polyclonal Anti Hcov Hku1 Spike Protein Antibody, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal anti hcov hku1 spike protein antibody/product/Sino Biological
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit polyclonal anti hcov hku1 spike protein antibody - by Bioz Stars, 2021-06
    93/100 stars
      Buy from Supplier

    94
    Sino Biological human coronavirus spike glycoprotein antibody mouse mab
    Infection of <t>human</t> lung cells from irradiated aerosolized beta HCoV-OC43 as function of dose of far-UVC light. Representative fluorescent images of WI-38 normal human lung fibroblasts infected with human betacoronavirus OC43 exposed in aerosolized form. The viral solution was collected from the BioSampler after running through the aerosol chamber while being exposed to ( a ) 0, ( b ) 0.5, ( c ) 1 or ( d ) 2 mJ/cm 2 of 222-nm light. Green fluorescence qualitatively indicates infected cells (Green = Alexa Fluor-488 used as secondary <t>antibody</t> against anti-human <t>coronavirus</t> <t>spike</t> <t>glycoprotein</t> antibody; Blue = nuclear stain DAPI). Images were acquired with a 10× objective; the scale bar applies to all the panels in the figure.
    Human Coronavirus Spike Glycoprotein Antibody Mouse Mab, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human coronavirus spike glycoprotein antibody mouse mab/product/Sino Biological
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human coronavirus spike glycoprotein antibody mouse mab - by Bioz Stars, 2021-06
    94/100 stars
      Buy from Supplier

    93
    Sino Biological human coronavirus spike glycoprotein antibody
    Infection of <t>human</t> lung cells from irradiated aerosolized beta HCoV-OC43 as function of dose of far-UVC light. Representative fluorescent images of WI-38 normal human lung fibroblasts infected with human betacoronavirus OC43 exposed in aerosolized form. The viral solution was collected from the BioSampler after running through the aerosol chamber while being exposed to ( a ) 0, ( b ) 0.5, ( c ) 1 or ( d ) 2 mJ/cm 2 of 222-nm light. Green fluorescence qualitatively indicates infected cells (Green = Alexa Fluor-488 used as secondary <t>antibody</t> against anti-human <t>coronavirus</t> <t>spike</t> <t>glycoprotein</t> antibody; Blue = nuclear stain DAPI). Images were acquired with a 10× objective; the scale bar applies to all the panels in the figure.
    Human Coronavirus Spike Glycoprotein Antibody, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human coronavirus spike glycoprotein antibody/product/Sino Biological
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human coronavirus spike glycoprotein antibody - by Bioz Stars, 2021-06
    93/100 stars
      Buy from Supplier

    Image Search Results


    Output of the panning of the phage-displayed synthetic Fab library on MERS-S2P. ( A ) Monitoring of phage titers over three rounds (R1–R3) of panning. Black and gray bars indicate a ratio of phage output to input titers presented as a percentage (%) from panning on MERS-S2P-immobilized and -non-immobilized surfaces, respectively. The ratio of output to input (%) = (phage output titer ÷ phage input titer) × 100. ( B ) Phage ELISAs performed on MERS-S2P-, SARS-CoV spike protein-, a CoV spike protein-immobilized surfaces (blue, red, and green, respectively). ( C ) Amino acid sequences of three unique clones identified from panning (left) and their relative frequencies (%) (right). The sequences were aligned using the Kabat numbering system [ 38 ]. ELISA, enzyme-linked immunosorbent assay; MERS-S2P, Middle East respiratory syndrome-CoV S2 subunit protein; SARS-SP, severe acute respiratory syndrome-CoV S protein; HKU1-SP, hCoV HKU1 S protein; CoV, coronavirus; CDR, complementarity-determining region; FR, framework region.

    Journal: Antibodies

    Article Title: Selection and Characterization of Monoclonal Antibodies Targeting Middle East Respiratory Syndrome Coronavirus through a Human Synthetic Fab Phage Display Library Panning

    doi: 10.3390/antib8030042

    Figure Lengend Snippet: Output of the panning of the phage-displayed synthetic Fab library on MERS-S2P. ( A ) Monitoring of phage titers over three rounds (R1–R3) of panning. Black and gray bars indicate a ratio of phage output to input titers presented as a percentage (%) from panning on MERS-S2P-immobilized and -non-immobilized surfaces, respectively. The ratio of output to input (%) = (phage output titer ÷ phage input titer) × 100. ( B ) Phage ELISAs performed on MERS-S2P-, SARS-CoV spike protein-, a CoV spike protein-immobilized surfaces (blue, red, and green, respectively). ( C ) Amino acid sequences of three unique clones identified from panning (left) and their relative frequencies (%) (right). The sequences were aligned using the Kabat numbering system [ 38 ]. ELISA, enzyme-linked immunosorbent assay; MERS-S2P, Middle East respiratory syndrome-CoV S2 subunit protein; SARS-SP, severe acute respiratory syndrome-CoV S protein; HKU1-SP, hCoV HKU1 S protein; CoV, coronavirus; CDR, complementarity-determining region; FR, framework region.

    Article Snippet: A mouse anti-229E coronavirus nucleoprotein OC-43 antibody (MERCK, Darmstadt, Germany), a mouse anti-coronavirus antibody, hCoV OC-43 (LifeSpan BioScience, Seattle, WA, USA), and a rabbit polyclonal anti-hCoV-HKU1 spike protein antibody (Sino Biological) were also incubated with MRC5 cells infected with the hCoV 229E or OC-43 strain, and with LLC-MK2 cells infected with the hCoV NL63 strain, with both types of cells used as positive controls.

    Techniques: Clone Assay, Enzyme-linked Immunosorbent Assay

    Infection of human lung cells from irradiated aerosolized beta HCoV-OC43 as function of dose of far-UVC light. Representative fluorescent images of WI-38 normal human lung fibroblasts infected with human betacoronavirus OC43 exposed in aerosolized form. The viral solution was collected from the BioSampler after running through the aerosol chamber while being exposed to ( a ) 0, ( b ) 0.5, ( c ) 1 or ( d ) 2 mJ/cm 2 of 222-nm light. Green fluorescence qualitatively indicates infected cells (Green = Alexa Fluor-488 used as secondary antibody against anti-human coronavirus spike glycoprotein antibody; Blue = nuclear stain DAPI). Images were acquired with a 10× objective; the scale bar applies to all the panels in the figure.

    Journal: Scientific Reports

    Article Title: Far-UVC light (222 nm) efficiently and safely inactivates airborne human coronaviruses

    doi: 10.1038/s41598-020-67211-2

    Figure Lengend Snippet: Infection of human lung cells from irradiated aerosolized beta HCoV-OC43 as function of dose of far-UVC light. Representative fluorescent images of WI-38 normal human lung fibroblasts infected with human betacoronavirus OC43 exposed in aerosolized form. The viral solution was collected from the BioSampler after running through the aerosol chamber while being exposed to ( a ) 0, ( b ) 0.5, ( c ) 1 or ( d ) 2 mJ/cm 2 of 222-nm light. Green fluorescence qualitatively indicates infected cells (Green = Alexa Fluor-488 used as secondary antibody against anti-human coronavirus spike glycoprotein antibody; Blue = nuclear stain DAPI). Images were acquired with a 10× objective; the scale bar applies to all the panels in the figure.

    Article Snippet: Infected cells were then fixed in 100% ice cold methanol at 4 °C for 5 minutes and labeled with anti-human coronavirus spike glycoprotein (40021-MM07, Sino Biologicals US Inc., Chesterbrook, PA) 1:200 in HBSS++ containing 1% bovine serum albumin (BSA; Sigma-Aldrich Corp. St. Louis, MO, USA) at room temperature for one hour with gentle shaking.

    Techniques: Infection, Irradiation, Fluorescence, Staining

    Infection of human lung cells from irradiated aerosolized alpha HCoV-229E as function of dose of far-UVC light. Representative fluorescent images of MRC-5 normal human lung fibroblasts infected with human alphacoronavirus 229E exposed in aerosolized form. The viral solution was collected from the BioSampler after running through the aerosol chamber while being exposed to ( a ) 0, ( b ) 0.5, ( c ) 1 or ( d ) 2 mJ/cm 2 of 222-nm light. Green fluorescence qualitatively indicates infected cells (Green = Alexa Fluor-488 used as secondary antibody against anti-human coronavirus spike glycoprotein antibody; Blue = nuclear stain DAPI). Images were acquired with a 10× objective; the scale bar applies to all the panels in the figure.

    Journal: Scientific Reports

    Article Title: Far-UVC light (222 nm) efficiently and safely inactivates airborne human coronaviruses

    doi: 10.1038/s41598-020-67211-2

    Figure Lengend Snippet: Infection of human lung cells from irradiated aerosolized alpha HCoV-229E as function of dose of far-UVC light. Representative fluorescent images of MRC-5 normal human lung fibroblasts infected with human alphacoronavirus 229E exposed in aerosolized form. The viral solution was collected from the BioSampler after running through the aerosol chamber while being exposed to ( a ) 0, ( b ) 0.5, ( c ) 1 or ( d ) 2 mJ/cm 2 of 222-nm light. Green fluorescence qualitatively indicates infected cells (Green = Alexa Fluor-488 used as secondary antibody against anti-human coronavirus spike glycoprotein antibody; Blue = nuclear stain DAPI). Images were acquired with a 10× objective; the scale bar applies to all the panels in the figure.

    Article Snippet: Infected cells were then fixed in 100% ice cold methanol at 4 °C for 5 minutes and labeled with anti-human coronavirus spike glycoprotein (40021-MM07, Sino Biologicals US Inc., Chesterbrook, PA) 1:200 in HBSS++ containing 1% bovine serum albumin (BSA; Sigma-Aldrich Corp. St. Louis, MO, USA) at room temperature for one hour with gentle shaking.

    Techniques: Infection, Irradiation, Fluorescence, Staining