human coronavirus spike glycoprotein antibody mouse mab  (Sino Biological)


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    Name:
    Human coronavirus spike glycoprotein Antibody Mouse MAb
    Description:
    This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified recombinant Human coronavirus spike glycoprotein Catalog 40021 V08H YP 173238 1 Met 1 Arg 760 The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography
    Catalog Number:
    40021-MM07
    Price:
    None
    Category:
    Primary Antibody
    Reactivity:
    HCoV HKU1
    Applications:
    ELISA
    Immunogen:
    Recombinant Human coronavirus spike glycoprotein protein (Catalog#40021-V08H)
    Antibody Type:
    MAb
    Host:
    Mouse
    Isotype:
    Mouse IgG1
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    Structured Review

    Sino Biological human coronavirus spike glycoprotein antibody mouse mab
    Infection of <t>human</t> lung cells from irradiated aerosolized beta HCoV-OC43 as function of dose of far-UVC light. Representative fluorescent images of WI-38 normal human lung fibroblasts infected with human betacoronavirus OC43 exposed in aerosolized form. The viral solution was collected from the BioSampler after running through the aerosol chamber while being exposed to ( a ) 0, ( b ) 0.5, ( c ) 1 or ( d ) 2 mJ/cm 2 of 222-nm light. Green fluorescence qualitatively indicates infected cells (Green = Alexa Fluor-488 used as secondary <t>antibody</t> against anti-human <t>coronavirus</t> <t>spike</t> <t>glycoprotein</t> antibody; Blue = nuclear stain DAPI). Images were acquired with a 10× objective; the scale bar applies to all the panels in the figure.
    This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified recombinant Human coronavirus spike glycoprotein Catalog 40021 V08H YP 173238 1 Met 1 Arg 760 The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography
    https://www.bioz.com/result/human coronavirus spike glycoprotein antibody mouse mab/product/Sino Biological
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human coronavirus spike glycoprotein antibody mouse mab - by Bioz Stars, 2021-06
    94/100 stars

    Images

    1) Product Images from "Far-UVC light (222 nm) efficiently and safely inactivates airborne human coronaviruses"

    Article Title: Far-UVC light (222 nm) efficiently and safely inactivates airborne human coronaviruses

    Journal: Scientific Reports

    doi: 10.1038/s41598-020-67211-2

    Infection of human lung cells from irradiated aerosolized beta HCoV-OC43 as function of dose of far-UVC light. Representative fluorescent images of WI-38 normal human lung fibroblasts infected with human betacoronavirus OC43 exposed in aerosolized form. The viral solution was collected from the BioSampler after running through the aerosol chamber while being exposed to ( a ) 0, ( b ) 0.5, ( c ) 1 or ( d ) 2 mJ/cm 2 of 222-nm light. Green fluorescence qualitatively indicates infected cells (Green = Alexa Fluor-488 used as secondary antibody against anti-human coronavirus spike glycoprotein antibody; Blue = nuclear stain DAPI). Images were acquired with a 10× objective; the scale bar applies to all the panels in the figure.
    Figure Legend Snippet: Infection of human lung cells from irradiated aerosolized beta HCoV-OC43 as function of dose of far-UVC light. Representative fluorescent images of WI-38 normal human lung fibroblasts infected with human betacoronavirus OC43 exposed in aerosolized form. The viral solution was collected from the BioSampler after running through the aerosol chamber while being exposed to ( a ) 0, ( b ) 0.5, ( c ) 1 or ( d ) 2 mJ/cm 2 of 222-nm light. Green fluorescence qualitatively indicates infected cells (Green = Alexa Fluor-488 used as secondary antibody against anti-human coronavirus spike glycoprotein antibody; Blue = nuclear stain DAPI). Images were acquired with a 10× objective; the scale bar applies to all the panels in the figure.

    Techniques Used: Infection, Irradiation, Fluorescence, Staining

    Infection of human lung cells from irradiated aerosolized alpha HCoV-229E as function of dose of far-UVC light. Representative fluorescent images of MRC-5 normal human lung fibroblasts infected with human alphacoronavirus 229E exposed in aerosolized form. The viral solution was collected from the BioSampler after running through the aerosol chamber while being exposed to ( a ) 0, ( b ) 0.5, ( c ) 1 or ( d ) 2 mJ/cm 2 of 222-nm light. Green fluorescence qualitatively indicates infected cells (Green = Alexa Fluor-488 used as secondary antibody against anti-human coronavirus spike glycoprotein antibody; Blue = nuclear stain DAPI). Images were acquired with a 10× objective; the scale bar applies to all the panels in the figure.
    Figure Legend Snippet: Infection of human lung cells from irradiated aerosolized alpha HCoV-229E as function of dose of far-UVC light. Representative fluorescent images of MRC-5 normal human lung fibroblasts infected with human alphacoronavirus 229E exposed in aerosolized form. The viral solution was collected from the BioSampler after running through the aerosol chamber while being exposed to ( a ) 0, ( b ) 0.5, ( c ) 1 or ( d ) 2 mJ/cm 2 of 222-nm light. Green fluorescence qualitatively indicates infected cells (Green = Alexa Fluor-488 used as secondary antibody against anti-human coronavirus spike glycoprotein antibody; Blue = nuclear stain DAPI). Images were acquired with a 10× objective; the scale bar applies to all the panels in the figure.

    Techniques Used: Infection, Irradiation, Fluorescence, Staining

    Related Articles

    Infection:

    Article Title: Far-UVC light (222 nm) efficiently and safely inactivates airborne human coronaviruses
    Article Snippet: The cells were incubated with the virus for one hour, then washed three times with HBSS++ , and then incubated overnight in fresh infection medium. .. Infected cells were then fixed in 100% ice cold methanol at 4 °C for 5 minutes and labeled with anti-human coronavirus spike glycoprotein (40021-MM07, Sino Biologicals US Inc., Chesterbrook, PA) 1:200 in HBSS++ containing 1% bovine serum albumin (BSA; Sigma-Aldrich Corp. St. Louis, MO, USA) at room temperature for one hour with gentle shaking. .. Cells were washed three times in HBSS++ and labeled with goat anti-mouse Alexa Fluor-488 (Life Technologies, Grand Island, NY) 1:800 in HBSS++ containing 1% BSA, at room temperature for 30 minutes in the dark with gentle shaking.

    Labeling:

    Article Title: Far-UVC light (222 nm) efficiently and safely inactivates airborne human coronaviruses
    Article Snippet: The cells were incubated with the virus for one hour, then washed three times with HBSS++ , and then incubated overnight in fresh infection medium. .. Infected cells were then fixed in 100% ice cold methanol at 4 °C for 5 minutes and labeled with anti-human coronavirus spike glycoprotein (40021-MM07, Sino Biologicals US Inc., Chesterbrook, PA) 1:200 in HBSS++ containing 1% bovine serum albumin (BSA; Sigma-Aldrich Corp. St. Louis, MO, USA) at room temperature for one hour with gentle shaking. .. Cells were washed three times in HBSS++ and labeled with goat anti-mouse Alexa Fluor-488 (Life Technologies, Grand Island, NY) 1:800 in HBSS++ containing 1% BSA, at room temperature for 30 minutes in the dark with gentle shaking.

    Transfection:

    Article Title: Characterization of spike glycoprotein of SARS-CoV-2 on virus entry and its immune cross-reactivity with SARS-CoV
    Article Snippet: Detection of S protein of SARS-CoV-2 by western blotThe spike glycoprotein of SARS-CoV-2 in cells and on pseudovirions were detected by using western blot. .. Briefly, cells transfected with plasmids encoding spike glycoprotein of SARS-CoV-2, SARS-CoV, MERS-CoV, and MHV were lysed at 40 h post transfection by RIPA buffer (20 mM Tris-HCl pH 7.5, 150 mM NaCl, 1 mM EDTA, 0.1% SDS, 1% NP40, 1×protease inhibitor cocktail). .. To pellet down pseudovirions, the viral supernatants were centrifuged at 25,000 rpm for 2 h in a Beckman SW41 rotor at 4 °C through a 20% sucrose cushion, and virus pellets were resuspended into 30 μl RIPA buffer.

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    Sino Biological human coronavirus spike glycoprotein antibody mouse mab
    Infection of <t>human</t> lung cells from irradiated aerosolized beta HCoV-OC43 as function of dose of far-UVC light. Representative fluorescent images of WI-38 normal human lung fibroblasts infected with human betacoronavirus OC43 exposed in aerosolized form. The viral solution was collected from the BioSampler after running through the aerosol chamber while being exposed to ( a ) 0, ( b ) 0.5, ( c ) 1 or ( d ) 2 mJ/cm 2 of 222-nm light. Green fluorescence qualitatively indicates infected cells (Green = Alexa Fluor-488 used as secondary <t>antibody</t> against anti-human <t>coronavirus</t> <t>spike</t> <t>glycoprotein</t> antibody; Blue = nuclear stain DAPI). Images were acquired with a 10× objective; the scale bar applies to all the panels in the figure.
    Human Coronavirus Spike Glycoprotein Antibody Mouse Mab, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human coronavirus spike glycoprotein antibody mouse mab/product/Sino Biological
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human coronavirus spike glycoprotein antibody mouse mab - by Bioz Stars, 2021-06
    94/100 stars
      Buy from Supplier

    Image Search Results


    Infection of human lung cells from irradiated aerosolized beta HCoV-OC43 as function of dose of far-UVC light. Representative fluorescent images of WI-38 normal human lung fibroblasts infected with human betacoronavirus OC43 exposed in aerosolized form. The viral solution was collected from the BioSampler after running through the aerosol chamber while being exposed to ( a ) 0, ( b ) 0.5, ( c ) 1 or ( d ) 2 mJ/cm 2 of 222-nm light. Green fluorescence qualitatively indicates infected cells (Green = Alexa Fluor-488 used as secondary antibody against anti-human coronavirus spike glycoprotein antibody; Blue = nuclear stain DAPI). Images were acquired with a 10× objective; the scale bar applies to all the panels in the figure.

    Journal: Scientific Reports

    Article Title: Far-UVC light (222 nm) efficiently and safely inactivates airborne human coronaviruses

    doi: 10.1038/s41598-020-67211-2

    Figure Lengend Snippet: Infection of human lung cells from irradiated aerosolized beta HCoV-OC43 as function of dose of far-UVC light. Representative fluorescent images of WI-38 normal human lung fibroblasts infected with human betacoronavirus OC43 exposed in aerosolized form. The viral solution was collected from the BioSampler after running through the aerosol chamber while being exposed to ( a ) 0, ( b ) 0.5, ( c ) 1 or ( d ) 2 mJ/cm 2 of 222-nm light. Green fluorescence qualitatively indicates infected cells (Green = Alexa Fluor-488 used as secondary antibody against anti-human coronavirus spike glycoprotein antibody; Blue = nuclear stain DAPI). Images were acquired with a 10× objective; the scale bar applies to all the panels in the figure.

    Article Snippet: Infected cells were then fixed in 100% ice cold methanol at 4 °C for 5 minutes and labeled with anti-human coronavirus spike glycoprotein (40021-MM07, Sino Biologicals US Inc., Chesterbrook, PA) 1:200 in HBSS++ containing 1% bovine serum albumin (BSA; Sigma-Aldrich Corp. St. Louis, MO, USA) at room temperature for one hour with gentle shaking.

    Techniques: Infection, Irradiation, Fluorescence, Staining

    Infection of human lung cells from irradiated aerosolized alpha HCoV-229E as function of dose of far-UVC light. Representative fluorescent images of MRC-5 normal human lung fibroblasts infected with human alphacoronavirus 229E exposed in aerosolized form. The viral solution was collected from the BioSampler after running through the aerosol chamber while being exposed to ( a ) 0, ( b ) 0.5, ( c ) 1 or ( d ) 2 mJ/cm 2 of 222-nm light. Green fluorescence qualitatively indicates infected cells (Green = Alexa Fluor-488 used as secondary antibody against anti-human coronavirus spike glycoprotein antibody; Blue = nuclear stain DAPI). Images were acquired with a 10× objective; the scale bar applies to all the panels in the figure.

    Journal: Scientific Reports

    Article Title: Far-UVC light (222 nm) efficiently and safely inactivates airborne human coronaviruses

    doi: 10.1038/s41598-020-67211-2

    Figure Lengend Snippet: Infection of human lung cells from irradiated aerosolized alpha HCoV-229E as function of dose of far-UVC light. Representative fluorescent images of MRC-5 normal human lung fibroblasts infected with human alphacoronavirus 229E exposed in aerosolized form. The viral solution was collected from the BioSampler after running through the aerosol chamber while being exposed to ( a ) 0, ( b ) 0.5, ( c ) 1 or ( d ) 2 mJ/cm 2 of 222-nm light. Green fluorescence qualitatively indicates infected cells (Green = Alexa Fluor-488 used as secondary antibody against anti-human coronavirus spike glycoprotein antibody; Blue = nuclear stain DAPI). Images were acquired with a 10× objective; the scale bar applies to all the panels in the figure.

    Article Snippet: Infected cells were then fixed in 100% ice cold methanol at 4 °C for 5 minutes and labeled with anti-human coronavirus spike glycoprotein (40021-MM07, Sino Biologicals US Inc., Chesterbrook, PA) 1:200 in HBSS++ containing 1% bovine serum albumin (BSA; Sigma-Aldrich Corp. St. Louis, MO, USA) at room temperature for one hour with gentle shaking.

    Techniques: Infection, Irradiation, Fluorescence, Staining