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Proliferation and Immunofluorescence. (a) CCK‐8 assay, Proliferation of HDF‐Htert in the control and in the presence of TVE and HA at 24, 48, and 72 h. (b) Immunofluorescence of COL1A1, COL3A1, and ELS staining after 24 h of the treatments. Magnification: 400×. ∗ Indicates p < 0.05 compared to CTR.

Journal: Advances in Pharmacological and Pharmaceutical Sciences

Article Title: Evaluation of Biological Effect of Triticum vulgare Extract Compared With Hyaluronic Acid in In Vitro Tissue Models

doi: 10.1155/adpp/8857215

Figure Lengend Snippet: Proliferation and Immunofluorescence. (a) CCK‐8 assay, Proliferation of HDF‐Htert in the control and in the presence of TVE and HA at 24, 48, and 72 h. (b) Immunofluorescence of COL1A1, COL3A1, and ELS staining after 24 h of the treatments. Magnification: 400×. ∗ Indicates p < 0.05 compared to CTR.

Article Snippet: Human keratinocytes (HaCaT) (supplied by “Zooprofilattico Institute”, Brescia, Italy) and HDF cell line immortalized with hTERT (HDF cells, BJ‐5ta, ATCC CRL‐4001), were cultured in Dulbecco’s modified Eagle medium (DMEM), supplemented with 10% fetal bovine serum (FBS), 1% penicillin–streptomycin, 1% gentamicin, and 1% amphotericin B.

Techniques: Immunofluorescence, CCK-8 Assay, Control, Staining

Dehydration test (a) Viability assay by MTT test. Reparative effects in HaCaT and HDF (b). Cell viability by MTT assay. ∗ p < 0.05, ∗∗ p < 0.01 compared to CTR‐ and # p < 0.05 compared to HA.

Journal: Advances in Pharmacological and Pharmaceutical Sciences

Article Title: Evaluation of Biological Effect of Triticum vulgare Extract Compared With Hyaluronic Acid in In Vitro Tissue Models

doi: 10.1155/adpp/8857215

Figure Lengend Snippet: Dehydration test (a) Viability assay by MTT test. Reparative effects in HaCaT and HDF (b). Cell viability by MTT assay. ∗ p < 0.05, ∗∗ p < 0.01 compared to CTR‐ and # p < 0.05 compared to HA.

Article Snippet: Human keratinocytes (HaCaT) (supplied by “Zooprofilattico Institute”, Brescia, Italy) and HDF cell line immortalized with hTERT (HDF cells, BJ‐5ta, ATCC CRL‐4001), were cultured in Dulbecco’s modified Eagle medium (DMEM), supplemented with 10% fetal bovine serum (FBS), 1% penicillin–streptomycin, 1% gentamicin, and 1% amphotericin B.

Techniques: Viability Assay, MTT Assay