human influenza viruses  (Sino Biological)


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    Name:
    Influenza A H1N1 Hemagglutinin HA HEK293 Cell Lysate
    Description:
    Human Cell lysate that Influenza A H1N1 A California 04 2009 Hemagglutinin transfected overexpressed for Western blot WB positive control The whole cell lysate is provided in 1X Sample Buffer 1X modified RIPA buffer 1X SDS loading buffer
    Catalog Number:
    11055-v08hl
    Price:
    195
    Applications:
    WB
    Size:
    300µg
    Category:
    Lysate
    Cell Type:
    HEK293 Cells
    Buy from Supplier


    Structured Review

    Sino Biological human influenza viruses
    Human Cell lysate that Influenza A H1N1 A California 04 2009 Hemagglutinin transfected overexpressed for Western blot WB positive control The whole cell lysate is provided in 1X Sample Buffer 1X modified RIPA buffer 1X SDS loading buffer
    https://www.bioz.com/result/human influenza viruses/product/Sino Biological
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human influenza viruses - by Bioz Stars, 2021-02
    92/100 stars

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    Related Articles

    Transfection:

    Article Title: Isoform-specific characterization of class I histone deacetylases and their therapeutic modulation in pulmonary hypertension
    Article Snippet: .. Transient overexpressionHuman vascular cells or ECV304 or HEK293 were transiently transfected with specific plasmids overexpressing HDAC1 (Sino Biological Inc.), HDAC2 (received as a gift from Prof. Edward Seto, George Washington Cancer Center), HDAC8 (OriGene Technologies Inc.) or LEF1 (Sino Biological Inc.) using either Amaxa Basic Nucleofecto Kit for Primary Mammalian Fibroblasts or TurboFect Transfection Reagent (Thermo Scientific), according to the manufacturer’s protocol. .. As a negative control, empty vector plasmids were used.

    Article Title: SMC5/6 acts jointly with Fanconi anemia factors to support DNA repair and genome stability
    Article Snippet: .. For transfection, HEK293 suspension cells were cultured in SMM 293‐TI medium (Sino Biological Inc.) supplemented with 1% Gibco FBS and 1% P/S in an 37°C incubator with shaking at 140 r.p.m. .. Cells were directly lysed with NTEN buffer [20 mM Tris–HCl (pH 7.5), 150 mM NaCl, 10% glycerol, 0.5% NP40, 10 mM NaF, 1 mM PMSF, 1 μg/ml leupeptin, 1 μg/ml aprotinin] 2 days after transfection.

    Article Title: The environment-sensing aryl-hydrocarbon receptor inhibits the chondrogenic fate of modulated smooth muscle cells in atherosclerotic lesions
    Article Snippet: .. Co-immunoprecipitation (co-IP) and ChIP-Western Blot HEK293 cells were transfected with plasmids carrying HA-tagged AHR cDNA (HG10456-CY, Sino Biological) and MYC-tagged TCF21 cDNA (HG16907-CM, Sino Biological). ..

    Article Title: Proteomics Reveals that Methylmalonyl-CoA Mutase Modulates Cell Architecture and Increases Susceptibility to Stress
    Article Snippet: .. In order to rescue the expression of MUT protein in MUT-knocked out cells, MUT-KO clone 2 was transfected as described above (for HEK 293 cells), with 24 µg of “pCMV3-Flag-MUT” plasmid (Sino Biological, Beijing, China). .. After 48 h from the transfection, the culture medium was replaced with a selective medium containing 1 µg/mL puromycin and 150 µg/mL hygromycin.

    Co-Immunoprecipitation Assay:

    Article Title: The environment-sensing aryl-hydrocarbon receptor inhibits the chondrogenic fate of modulated smooth muscle cells in atherosclerotic lesions
    Article Snippet: .. Co-immunoprecipitation (co-IP) and ChIP-Western Blot HEK293 cells were transfected with plasmids carrying HA-tagged AHR cDNA (HG10456-CY, Sino Biological) and MYC-tagged TCF21 cDNA (HG16907-CM, Sino Biological). ..

    Cell Culture:

    Article Title: SMC5/6 acts jointly with Fanconi anemia factors to support DNA repair and genome stability
    Article Snippet: .. For transfection, HEK293 suspension cells were cultured in SMM 293‐TI medium (Sino Biological Inc.) supplemented with 1% Gibco FBS and 1% P/S in an 37°C incubator with shaking at 140 r.p.m. .. Cells were directly lysed with NTEN buffer [20 mM Tris–HCl (pH 7.5), 150 mM NaCl, 10% glycerol, 0.5% NP40, 10 mM NaF, 1 mM PMSF, 1 μg/ml leupeptin, 1 μg/ml aprotinin] 2 days after transfection.

    Mouse Assay:

    Article Title: Epitope mapping of the 2009 pandemic and the A/Brisbane/59/2007 seasonal (H1N1) influenza virus haemagglutinins using mAbs and escape mutants.
    Article Snippet: .. Another group of mice was immunized as per the 11 previous protocol but with an A/Brisbane/59/2007-like isolate and then with the recombinant Bris07 HA 12 produced in human HEK293 cells (Sino Biologicals). ..

    Produced:

    Article Title: Epitope mapping of the 2009 pandemic and the A/Brisbane/59/2007 seasonal (H1N1) influenza virus haemagglutinins using mAbs and escape mutants.
    Article Snippet: .. Three weeks later, a s.c. boost of FIA with 10 µg of recombinant 8 A/California/04/2009 HA protein (recHA) produced in HEK293 human cells (Sino Biologicals, Beijing, China) 9 was performed. ..

    Article Title: Epitope mapping of the 2009 pandemic and the A/Brisbane/59/2007 seasonal (H1N1) influenza virus haemagglutinins using mAbs and escape mutants.
    Article Snippet: .. Another group of mice was immunized as per the 11 previous protocol but with an A/Brisbane/59/2007-like isolate and then with the recombinant Bris07 HA 12 produced in human HEK293 cells (Sino Biologicals). ..

    Derivative Assay:

    Article Title: Kallistatin Ameliorates Influenza Virus Pathogenesis by Inhibition of Kallikrein-Related Peptidase 1-Mediated Cleavage of Viral Hemagglutinin
    Article Snippet: .. Recombinant HA proteins (100 ng in 20 μl saline) derived from influenza A/WSN/33 (H1N1), A/California/04/09 (H1N1), A/Japan/305/57 (H2N2), and A/Brisbane/10/07 (H3N2) viruses were purchased from Sino Biological Inc. (Beijing, China). .. They were incubated with KLK1 at concentrations ranging from 0.1 to 1 μg/ml for 3 or 4 h at 37°C.

    Expressing:

    Article Title: Proteomics Reveals that Methylmalonyl-CoA Mutase Modulates Cell Architecture and Increases Susceptibility to Stress
    Article Snippet: .. In order to rescue the expression of MUT protein in MUT-knocked out cells, MUT-KO clone 2 was transfected as described above (for HEK 293 cells), with 24 µg of “pCMV3-Flag-MUT” plasmid (Sino Biological, Beijing, China). .. After 48 h from the transfection, the culture medium was replaced with a selective medium containing 1 µg/mL puromycin and 150 µg/mL hygromycin.

    Recombinant:

    Article Title: Epitope mapping of the 2009 pandemic and the A/Brisbane/59/2007 seasonal (H1N1) influenza virus haemagglutinins using mAbs and escape mutants.
    Article Snippet: .. Three weeks later, a s.c. boost of FIA with 10 µg of recombinant 8 A/California/04/2009 HA protein (recHA) produced in HEK293 human cells (Sino Biologicals, Beijing, China) 9 was performed. ..

    Article Title: Epitope mapping of the 2009 pandemic and the A/Brisbane/59/2007 seasonal (H1N1) influenza virus haemagglutinins using mAbs and escape mutants.
    Article Snippet: .. Another group of mice was immunized as per the 11 previous protocol but with an A/Brisbane/59/2007-like isolate and then with the recombinant Bris07 HA 12 produced in human HEK293 cells (Sino Biologicals). ..

    Article Title: Kallistatin Ameliorates Influenza Virus Pathogenesis by Inhibition of Kallikrein-Related Peptidase 1-Mediated Cleavage of Viral Hemagglutinin
    Article Snippet: .. Recombinant HA proteins (100 ng in 20 μl saline) derived from influenza A/WSN/33 (H1N1), A/California/04/09 (H1N1), A/Japan/305/57 (H2N2), and A/Brisbane/10/07 (H3N2) viruses were purchased from Sino Biological Inc. (Beijing, China). .. They were incubated with KLK1 at concentrations ranging from 0.1 to 1 μg/ml for 3 or 4 h at 37°C.

    Chromatin Immunoprecipitation:

    Article Title: The environment-sensing aryl-hydrocarbon receptor inhibits the chondrogenic fate of modulated smooth muscle cells in atherosclerotic lesions
    Article Snippet: .. Co-immunoprecipitation (co-IP) and ChIP-Western Blot HEK293 cells were transfected with plasmids carrying HA-tagged AHR cDNA (HG10456-CY, Sino Biological) and MYC-tagged TCF21 cDNA (HG16907-CM, Sino Biological). ..

    Plasmid Preparation:

    Article Title: Proteomics Reveals that Methylmalonyl-CoA Mutase Modulates Cell Architecture and Increases Susceptibility to Stress
    Article Snippet: .. In order to rescue the expression of MUT protein in MUT-knocked out cells, MUT-KO clone 2 was transfected as described above (for HEK 293 cells), with 24 µg of “pCMV3-Flag-MUT” plasmid (Sino Biological, Beijing, China). .. After 48 h from the transfection, the culture medium was replaced with a selective medium containing 1 µg/mL puromycin and 150 µg/mL hygromycin.

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  • 92
    Sino Biological human influenza viruses
    Human Influenza Viruses, supplied by Sino Biological, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human influenza viruses/product/Sino Biological
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human influenza viruses - by Bioz Stars, 2021-02
    92/100 stars
      Buy from Supplier

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    Human Cell lysate that Influenza A H1N1 A New York 18 2009 HA transfected overexpressed for Western blot WB positive control The whole cell lysate is provided in 1X Sample
      Buy from Supplier

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