3xflag c fluc tagged ank1  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier
Bioz Manufacturer Symbol Thermo Fisher manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Thermo Fisher 3xflag c fluc tagged ank1
    PF3D7_0402000 (D90c) interacts with <t>ANK1</t> and 4.1R in co-precipitation assays A) and C) Expression of proteins tagged with C-terminal fragment of firefly luciferase protein <t>(C-FLuc)</t> and 3XFLAG. ANK1 FG1 and 4.1R FG1 as fusions to C-FLuc-3XFLAG and C-FLuc-3XFLAG with no insert were in vitro translated in wheat germ extracts (WGE) and subjected to western blotting with anti-FLAG tag antibody. Molecular weight markers (sizes in kDa) are indicated at left. B) Co-affinity purification of erythrocyte ankyrin and 4.1R with MBP-His-tagged PF3D7_0402000 (MBP-D90c-His). C-FLuc-3XFLAG-tagged proteins from (A) were incubated with MBP-D90c-His and MBP-His and purified with amylose resin. The co-purifying proteins were subjected to SDS-PAGE analysis and western blotting using anti-FLAG antibody (top panel) and anti-MBP antibody (bottom panel). Molecular weight markers (sizes in kDa) are indicated at left. D) Co-affinity purification of erythrocyte ankyrin and 4.1R with GST tagged-PF3D7_0402000 (GST-D90c). GST and GST-D90c were in vitro translated in WGE, incubated with C-FLuc-3XFLAG-tagged proteins from (C) and purified with GST beads. The co-purifying proteins were subjected to SDS-PAGE and western blotting using anti-FLAG (top panel) and anti-GST antibodies (bottom panel). Molecular weight markers (sizes in kDa) are indicated at left.
    3xflag C Fluc Tagged Ank1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/3xflag c fluc tagged ank1/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    3xflag c fluc tagged ank1 - by Bioz Stars, 2021-04
    86/100 stars

    Images

    1) Product Images from "The Plasmodium falciparum exported protein PF3D7_0402000 binds to erythrocyte ankyrin and band 4.1"

    Article Title: The Plasmodium falciparum exported protein PF3D7_0402000 binds to erythrocyte ankyrin and band 4.1

    Journal: Molecular and biochemical parasitology

    doi: 10.1016/j.molbiopara.2017.06.002

    PF3D7_0402000 (D90c) interacts with ANK1 and 4.1R in co-precipitation assays A) and C) Expression of proteins tagged with C-terminal fragment of firefly luciferase protein (C-FLuc) and 3XFLAG. ANK1 FG1 and 4.1R FG1 as fusions to C-FLuc-3XFLAG and C-FLuc-3XFLAG with no insert were in vitro translated in wheat germ extracts (WGE) and subjected to western blotting with anti-FLAG tag antibody. Molecular weight markers (sizes in kDa) are indicated at left. B) Co-affinity purification of erythrocyte ankyrin and 4.1R with MBP-His-tagged PF3D7_0402000 (MBP-D90c-His). C-FLuc-3XFLAG-tagged proteins from (A) were incubated with MBP-D90c-His and MBP-His and purified with amylose resin. The co-purifying proteins were subjected to SDS-PAGE analysis and western blotting using anti-FLAG antibody (top panel) and anti-MBP antibody (bottom panel). Molecular weight markers (sizes in kDa) are indicated at left. D) Co-affinity purification of erythrocyte ankyrin and 4.1R with GST tagged-PF3D7_0402000 (GST-D90c). GST and GST-D90c were in vitro translated in WGE, incubated with C-FLuc-3XFLAG-tagged proteins from (C) and purified with GST beads. The co-purifying proteins were subjected to SDS-PAGE and western blotting using anti-FLAG (top panel) and anti-GST antibodies (bottom panel). Molecular weight markers (sizes in kDa) are indicated at left.
    Figure Legend Snippet: PF3D7_0402000 (D90c) interacts with ANK1 and 4.1R in co-precipitation assays A) and C) Expression of proteins tagged with C-terminal fragment of firefly luciferase protein (C-FLuc) and 3XFLAG. ANK1 FG1 and 4.1R FG1 as fusions to C-FLuc-3XFLAG and C-FLuc-3XFLAG with no insert were in vitro translated in wheat germ extracts (WGE) and subjected to western blotting with anti-FLAG tag antibody. Molecular weight markers (sizes in kDa) are indicated at left. B) Co-affinity purification of erythrocyte ankyrin and 4.1R with MBP-His-tagged PF3D7_0402000 (MBP-D90c-His). C-FLuc-3XFLAG-tagged proteins from (A) were incubated with MBP-D90c-His and MBP-His and purified with amylose resin. The co-purifying proteins were subjected to SDS-PAGE analysis and western blotting using anti-FLAG antibody (top panel) and anti-MBP antibody (bottom panel). Molecular weight markers (sizes in kDa) are indicated at left. D) Co-affinity purification of erythrocyte ankyrin and 4.1R with GST tagged-PF3D7_0402000 (GST-D90c). GST and GST-D90c were in vitro translated in WGE, incubated with C-FLuc-3XFLAG-tagged proteins from (C) and purified with GST beads. The co-purifying proteins were subjected to SDS-PAGE and western blotting using anti-FLAG (top panel) and anti-GST antibodies (bottom panel). Molecular weight markers (sizes in kDa) are indicated at left.

    Techniques Used: Expressing, Luciferase, In Vitro, Western Blot, FLAG-tag, Molecular Weight, Affinity Purification, Incubation, Purification, SDS Page

    PF3D7_0402000 (D90c) targets the 2nd folding domain of ANK1 A) Expression of proteins tagged with the C-terminal fragment of firefly luciferase protein (C-FLuc) and 3XFLAG. D1, D2 and D1+D2 subdomains of ANK1 as fusions to C-FLuc-3XFLAG and C-FLuc-3XFLAG with no insert were in vitro translated in wheat germ extracts (WGE) and subjected to SDS-PAGE and western blotting with anti-FLAG tag antibody. Molecular weight markers (sizes in kDa) are indicated at left. B) Co-affinity purification of ANK1 subdomains with MBP-D90c-His. C-FLuc-3XFLAG-tagged proteins from (A) were incubated with MBP-D90c-His and MBP-His and purified with amylose resin. The co-purifying proteins were subjected to SDS-PAGE and western blotting using anti- FLAG (top panel) and anti-MBP antibodies (bottom panel). Molecular weight markers (sizes in kDa) are indicated at left.
    Figure Legend Snippet: PF3D7_0402000 (D90c) targets the 2nd folding domain of ANK1 A) Expression of proteins tagged with the C-terminal fragment of firefly luciferase protein (C-FLuc) and 3XFLAG. D1, D2 and D1+D2 subdomains of ANK1 as fusions to C-FLuc-3XFLAG and C-FLuc-3XFLAG with no insert were in vitro translated in wheat germ extracts (WGE) and subjected to SDS-PAGE and western blotting with anti-FLAG tag antibody. Molecular weight markers (sizes in kDa) are indicated at left. B) Co-affinity purification of ANK1 subdomains with MBP-D90c-His. C-FLuc-3XFLAG-tagged proteins from (A) were incubated with MBP-D90c-His and MBP-His and purified with amylose resin. The co-purifying proteins were subjected to SDS-PAGE and western blotting using anti- FLAG (top panel) and anti-MBP antibodies (bottom panel). Molecular weight markers (sizes in kDa) are indicated at left.

    Techniques Used: Expressing, Luciferase, In Vitro, SDS Page, Western Blot, FLAG-tag, Molecular Weight, Affinity Purification, Incubation, Purification

    Related Articles

    Incubation:

    Article Title: The Plasmodium falciparum exported protein PF3D7_0402000 binds to erythrocyte ankyrin and band 4.1
    Article Snippet: Glutathione S-transferase (GST) pull-down assays were performed with GST or GST-tagged PF3D7_0402000 (GST-D90c) that was in vitro translated using wheat germ extracts [ ]. .. GST or GST-D90c were mixed with 3XFLAG-C-FLuc tagged ANK1 and 4.1R, incubated with GST beads (Thermo Scientific) overnight at 4° C in presence of protease inhibitor (PI) cocktail (Roche), washed three times with PBS+0.1% TritonX-100, eluted by boiling in Laemmli SDS-PAGE sample loading buffer, and subjected to western blot analysis. ..

    Protease Inhibitor:

    Article Title: The Plasmodium falciparum exported protein PF3D7_0402000 binds to erythrocyte ankyrin and band 4.1
    Article Snippet: Glutathione S-transferase (GST) pull-down assays were performed with GST or GST-tagged PF3D7_0402000 (GST-D90c) that was in vitro translated using wheat germ extracts [ ]. .. GST or GST-D90c were mixed with 3XFLAG-C-FLuc tagged ANK1 and 4.1R, incubated with GST beads (Thermo Scientific) overnight at 4° C in presence of protease inhibitor (PI) cocktail (Roche), washed three times with PBS+0.1% TritonX-100, eluted by boiling in Laemmli SDS-PAGE sample loading buffer, and subjected to western blot analysis. ..

    SDS Page:

    Article Title: The Plasmodium falciparum exported protein PF3D7_0402000 binds to erythrocyte ankyrin and band 4.1
    Article Snippet: Glutathione S-transferase (GST) pull-down assays were performed with GST or GST-tagged PF3D7_0402000 (GST-D90c) that was in vitro translated using wheat germ extracts [ ]. .. GST or GST-D90c were mixed with 3XFLAG-C-FLuc tagged ANK1 and 4.1R, incubated with GST beads (Thermo Scientific) overnight at 4° C in presence of protease inhibitor (PI) cocktail (Roche), washed three times with PBS+0.1% TritonX-100, eluted by boiling in Laemmli SDS-PAGE sample loading buffer, and subjected to western blot analysis. ..

    Western Blot:

    Article Title: The Plasmodium falciparum exported protein PF3D7_0402000 binds to erythrocyte ankyrin and band 4.1
    Article Snippet: Glutathione S-transferase (GST) pull-down assays were performed with GST or GST-tagged PF3D7_0402000 (GST-D90c) that was in vitro translated using wheat germ extracts [ ]. .. GST or GST-D90c were mixed with 3XFLAG-C-FLuc tagged ANK1 and 4.1R, incubated with GST beads (Thermo Scientific) overnight at 4° C in presence of protease inhibitor (PI) cocktail (Roche), washed three times with PBS+0.1% TritonX-100, eluted by boiling in Laemmli SDS-PAGE sample loading buffer, and subjected to western blot analysis. ..

    Similar Products

    No Results Found