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Santa Cruz Biotechnology 34hl
34hl, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 94 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/34hl/product/Santa Cruz Biotechnology
Average 93 stars, based on 94 article reviews
34hl - by Bioz Stars, 2026-06
93/100 stars

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34hl, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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a. Immunohistochemistry using monoclonal antibodies to DEFB1. The corresponding isotype controls were negative. Scale bar: 20 μm. Original magnification: x200.
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a. Immunohistochemistry using monoclonal antibodies to DEFB1. The corresponding isotype controls were negative. Scale bar: 20 μm. Original magnification: x200.
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a mRNA apoE, IL-1beta, and TREM2 expression was evaluated in microglial cells at 1, 3, 7, and 14 d.p.i. b HHV-6A-infected microglial cells (m.o.i. 100:1; 14 d.p.i.) were stained with <t>anti-Iba-1</t> FITC and TREM2 PE moAbs. Images were taken in bright field ( left panel ) or fluorescence ( right panels ) (Nikon Eclipse TE2000S) equipped with a digital camera. Original magnification × 20. c IL-1α, IL-1β, IL-6, IL-10, and tumor necrosis factor-α expression in uninfected (white histogram) and in HHV-6-infected microglial cells (gray histogram). * p value < 0.0001, obtained by Student’s t test. Each experiment was performed in triplicate
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a mRNA apoE, IL-1beta, and TREM2 expression was evaluated in microglial cells at 1, 3, 7, and 14 d.p.i. b HHV-6A-infected microglial cells (m.o.i. 100:1; 14 d.p.i.) were stained with <t>anti-Iba-1</t> FITC and TREM2 PE moAbs. Images were taken in bright field ( left panel ) or fluorescence ( right panels ) (Nikon Eclipse TE2000S) equipped with a digital camera. Original magnification × 20. c IL-1α, IL-1β, IL-6, IL-10, and tumor necrosis factor-α expression in uninfected (white histogram) and in HHV-6-infected microglial cells (gray histogram). * p value < 0.0001, obtained by Student’s t test. Each experiment was performed in triplicate
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a mRNA apoE, IL-1beta, and TREM2 expression was evaluated in microglial cells at 1, 3, 7, and 14 d.p.i. b HHV-6A-infected microglial cells (m.o.i. 100:1; 14 d.p.i.) were stained with <t>anti-Iba-1</t> FITC and TREM2 PE moAbs. Images were taken in bright field ( left panel ) or fluorescence ( right panels ) (Nikon Eclipse TE2000S) equipped with a digital camera. Original magnification × 20. c IL-1α, IL-1β, IL-6, IL-10, and tumor necrosis factor-α expression in uninfected (white histogram) and in HHV-6-infected microglial cells (gray histogram). * p value < 0.0001, obtained by Student’s t test. Each experiment was performed in triplicate
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a mRNA apoE, IL-1beta, and TREM2 expression was evaluated in microglial cells at 1, 3, 7, and 14 d.p.i. b HHV-6A-infected microglial cells (m.o.i. 100:1; 14 d.p.i.) were stained with <t>anti-Iba-1</t> FITC and TREM2 PE moAbs. Images were taken in bright field ( left panel ) or fluorescence ( right panels ) (Nikon Eclipse TE2000S) equipped with a digital camera. Original magnification × 20. c IL-1α, IL-1β, IL-6, IL-10, and tumor necrosis factor-α expression in uninfected (white histogram) and in HHV-6-infected microglial cells (gray histogram). * p value < 0.0001, obtained by Student’s t test. Each experiment was performed in triplicate
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a mRNA apoE, IL-1beta, and TREM2 expression was evaluated in microglial cells at 1, 3, 7, and 14 d.p.i. b HHV-6A-infected microglial cells (m.o.i. 100:1; 14 d.p.i.) were stained with <t>anti-Iba-1</t> FITC and TREM2 PE moAbs. Images were taken in bright field ( left panel ) or fluorescence ( right panels ) (Nikon Eclipse TE2000S) equipped with a digital camera. Original magnification × 20. c IL-1α, IL-1β, IL-6, IL-10, and tumor necrosis factor-α expression in uninfected (white histogram) and in HHV-6-infected microglial cells (gray histogram). * p value < 0.0001, obtained by Student’s t test. Each experiment was performed in triplicate
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a mRNA apoE, IL-1beta, and TREM2 expression was evaluated in microglial cells at 1, 3, 7, and 14 d.p.i. b HHV-6A-infected microglial cells (m.o.i. 100:1; 14 d.p.i.) were stained with <t>anti-Iba-1</t> FITC and TREM2 PE moAbs. Images were taken in bright field ( left panel ) or fluorescence ( right panels ) (Nikon Eclipse TE2000S) equipped with a digital camera. Original magnification × 20. c IL-1α, IL-1β, IL-6, IL-10, and tumor necrosis factor-α expression in uninfected (white histogram) and in HHV-6-infected microglial cells (gray histogram). * p value < 0.0001, obtained by Student’s t test. Each experiment was performed in triplicate
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a mRNA apoE, IL-1beta, and TREM2 expression was evaluated in microglial cells at 1, 3, 7, and 14 d.p.i. b HHV-6A-infected microglial cells (m.o.i. 100:1; 14 d.p.i.) were stained with <t>anti-Iba-1</t> FITC and TREM2 PE moAbs. Images were taken in bright field ( left panel ) or fluorescence ( right panels ) (Nikon Eclipse TE2000S) equipped with a digital camera. Original magnification × 20. c IL-1α, IL-1β, IL-6, IL-10, and tumor necrosis factor-α expression in uninfected (white histogram) and in HHV-6-infected microglial cells (gray histogram). * p value < 0.0001, obtained by Student’s t test. Each experiment was performed in triplicate
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a. Immunohistochemistry using monoclonal antibodies to DEFB1. The corresponding isotype controls were negative. Scale bar: 20 μm. Original magnification: x200.

Journal: Nature immunology

Article Title: The Cellular Architecture of the Antimicrobial Response Network in Human Leprosy Granulomas

doi: 10.1038/s41590-021-00956-8

Figure Lengend Snippet: a. Immunohistochemistry using monoclonal antibodies to DEFB1. The corresponding isotype controls were negative. Scale bar: 20 μm. Original magnification: x200.

Article Snippet: Frozen tissue sections from three RR and three L-lep specimens were blocked with normal horse serum before incubation with DEFB1 (clone M11–14b-D10, Abcam), CXCL14 (clone {"type":"entrez-protein","attrs":{"text":"EPR22807","term_id":"523390812","term_text":"EPR22807"}} EPR22807 –28, Abcam) and TAC1 (clone NC1/34HL, Santa Cruz) monoclonal antibodies, and respective isotype controls (Sigma-Aldrich) for 60 minutes, followed by incubation with biotinylated secondary antibodies (Vector Laboratories) for 30 minutes.

Techniques: Immunohistochemistry, Biomarker Discovery, Expressing, Bioprocessing

a. Immunohistochemistry using monoclonal antibodies to DEFB1. The corresponding isotype controls were negative. Scale bar: 20 μm. Original magnification: x200.

Journal: Nature immunology

Article Title: The Cellular Architecture of the Antimicrobial Response Network in Human Leprosy Granulomas

doi: 10.1038/s41590-021-00956-8

Figure Lengend Snippet: a. Immunohistochemistry using monoclonal antibodies to DEFB1. The corresponding isotype controls were negative. Scale bar: 20 μm. Original magnification: x200.

Article Snippet: Immunohistochemistry Frozen tissue sections from three RR and three L-lep specimens were blocked with normal horse serum before incubation with DEFB1 (clone M11–14b-D10, Abcam), CXCL14 (clone {"type":"entrez-protein","attrs":{"text":"EPR22807","term_id":"523390812","term_text":"EPR22807"}} EPR22807 –28, Abcam) and TAC1 (clone NC1/34HL, Santa Cruz) monoclonal antibodies, and respective isotype controls (Sigma-Aldrich) for 60 minutes, followed by incubation with biotinylated secondary antibodies (Vector Laboratories) for 30 minutes.

Techniques: Immunohistochemistry, Expressing

a mRNA apoE, IL-1beta, and TREM2 expression was evaluated in microglial cells at 1, 3, 7, and 14 d.p.i. b HHV-6A-infected microglial cells (m.o.i. 100:1; 14 d.p.i.) were stained with anti-Iba-1 FITC and TREM2 PE moAbs. Images were taken in bright field ( left panel ) or fluorescence ( right panels ) (Nikon Eclipse TE2000S) equipped with a digital camera. Original magnification × 20. c IL-1α, IL-1β, IL-6, IL-10, and tumor necrosis factor-α expression in uninfected (white histogram) and in HHV-6-infected microglial cells (gray histogram). * p value < 0.0001, obtained by Student’s t test. Each experiment was performed in triplicate

Journal: Alzheimer's Research & Therapy

Article Title: HHV-6A infection induces amyloid-beta expression and activation of microglial cells

doi: 10.1186/s13195-019-0552-6

Figure Lengend Snippet: a mRNA apoE, IL-1beta, and TREM2 expression was evaluated in microglial cells at 1, 3, 7, and 14 d.p.i. b HHV-6A-infected microglial cells (m.o.i. 100:1; 14 d.p.i.) were stained with anti-Iba-1 FITC and TREM2 PE moAbs. Images were taken in bright field ( left panel ) or fluorescence ( right panels ) (Nikon Eclipse TE2000S) equipped with a digital camera. Original magnification × 20. c IL-1α, IL-1β, IL-6, IL-10, and tumor necrosis factor-α expression in uninfected (white histogram) and in HHV-6-infected microglial cells (gray histogram). * p value < 0.0001, obtained by Student’s t test. Each experiment was performed in triplicate

Article Snippet: Microglial cells were stained with anti-substance P FITC (NC1/34HL) mAb anti-Iba-1 PE (1022-5) mAb, anti-TREM2 PE mAb (B-3) (Santa Cruz Biotechnology, USA), anti-Aβ 1-40 (NBP1-44047; Novus Biologicals; Italy), and Aβ 1-42 moAb (ab10148; Abcam; UK).

Techniques: Expressing, Infection, Staining, Fluorescence