Journal: Alzheimer's Research & Therapy
Article Title: HHV-6A infection induces amyloid-beta expression and activation of microglial cells
doi: 10.1186/s13195-019-0552-6
Figure Lengend Snippet: a mRNA apoE, IL-1beta, and TREM2 expression was evaluated in microglial cells at 1, 3, 7, and 14 d.p.i. b HHV-6A-infected microglial cells (m.o.i. 100:1; 14 d.p.i.) were stained with anti-Iba-1 FITC and TREM2 PE moAbs. Images were taken in bright field ( left panel ) or fluorescence ( right panels ) (Nikon Eclipse TE2000S) equipped with a digital camera. Original magnification × 20. c IL-1α, IL-1β, IL-6, IL-10, and tumor necrosis factor-α expression in uninfected (white histogram) and in HHV-6-infected microglial cells (gray histogram). * p value < 0.0001, obtained by Student’s t test. Each experiment was performed in triplicate
Article Snippet: Microglial cells were stained with anti-substance P FITC (NC1/34HL) mAb anti-Iba-1 PE (1022-5) mAb, anti-TREM2 PE mAb (B-3) (Santa Cruz Biotechnology, USA), anti-Aβ 1-40 (NBP1-44047; Novus Biologicals; Italy), and Aβ 1-42 moAb (ab10148; Abcam; UK).
Techniques: Expressing, Infection, Staining, Fluorescence