mirnas  (Qiagen)


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    Name:
    QIAseq miRNA Library Kit
    Description:
    Gel free miRNA Sample to Insight solution for differential expression analysis and novel discovery using next generation sequencing Kit contents For 12 sequencing prep reactions 3 ligation 5 ligation reverse transcription cDNA cleanup library amplification and library cleanup reagents quality control primers Benefits Gel free miRNA sequencing library prep from as little as 1 ng of total RNA Elimination of adapter dimers and unwanted RNA species resulting in the highest fidelity and most efficient data Bead based method to remove adapter dimers and unwanted RNA species Integrated Unique Molecular Indices UMIs enable quantification of individual miRNA molecules Primary read mapping and differential expression analysis via the GeneGlobe Data Analysis Cen
    Catalog Number:
    331502
    Price:
    1120
    Category:
    QIAseq miRNA Library Kit
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    Structured Review

    Qiagen mirnas
    QIAseq miRNA Library Kit
    Gel free miRNA Sample to Insight solution for differential expression analysis and novel discovery using next generation sequencing Kit contents For 12 sequencing prep reactions 3 ligation 5 ligation reverse transcription cDNA cleanup library amplification and library cleanup reagents quality control primers Benefits Gel free miRNA sequencing library prep from as little as 1 ng of total RNA Elimination of adapter dimers and unwanted RNA species resulting in the highest fidelity and most efficient data Bead based method to remove adapter dimers and unwanted RNA species Integrated Unique Molecular Indices UMIs enable quantification of individual miRNA molecules Primary read mapping and differential expression analysis via the GeneGlobe Data Analysis Cen
    https://www.bioz.com/result/mirnas/product/Qiagen
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mirnas - by Bioz Stars, 2021-03
    99/100 stars

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    1) Product Images from "Human bronchial epithelial cell-derived extracellular vesicle therapy for pulmonary fibrosis via inhibition of TGF-β-WNT crosstalk"

    Article Title: Human bronchial epithelial cell-derived extracellular vesicle therapy for pulmonary fibrosis via inhibition of TGF-β-WNT crosstalk

    Journal: bioRxiv

    doi: 10.1101/2020.10.22.349761

    miRNA cargo in HBEC EVs attenuates TGF-β-induced epithelial cell senescence via suppression of WNT3A and WNT5A expression . A Representative immunoblot showing the amount of active β-catenin and β-actin in HBECs treated for 48 h with PBS or HBEC EVs (10 μg/ml) in the presence or absence of TGF-β1 (2 ng/ml). B qRT-PCR analysis of WNT1, WNT3A, WNT5A and WNT10B mRNAs in HBECs treated for 24 h with HBEC EVs (10 μg/ml, n=3). * P
    Figure Legend Snippet: miRNA cargo in HBEC EVs attenuates TGF-β-induced epithelial cell senescence via suppression of WNT3A and WNT5A expression . A Representative immunoblot showing the amount of active β-catenin and β-actin in HBECs treated for 48 h with PBS or HBEC EVs (10 μg/ml) in the presence or absence of TGF-β1 (2 ng/ml). B qRT-PCR analysis of WNT1, WNT3A, WNT5A and WNT10B mRNAs in HBECs treated for 24 h with HBEC EVs (10 μg/ml, n=3). * P

    Techniques Used: Expressing, Quantitative RT-PCR

    HBEC EVs cargo has a unique miRNA profile. A Schematic protocol for the mass spectrometry (MS) and miRNA-seq approach to characterizing EV cargo. EVs were isolated from HBEC and BEAS-2B for MS analysis and miRNA-seq analysis. B Summary of the 30 most abundant miRNAs in HBEC EVs. Data represent read counts for each miRNA species in the miRNA-seq analysis. n= 2. C Relative expression of the 30 most abundant miRNA species in the miRNA microarray dataset. Bars represent the log 2 fold-change in expression of each miRNA, with positive fold-change values indicating higher levels in IPF lung than non-disease lung. Error bars are omitted for clarity. The Gene Expression Omnibus (GEO) database GSE32538 contains 106 IPF/usual interstitial pneumonitis (UIP) samples and 50 non-diseased samples. D Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways from DIANA-mirPath v3.0 analysis, based on the 30 most abundant miRNAs in HBEC EVs. E Gene Ontology from DIANA-mirPath v3.0 analysis, based on the 30 most abundant miRNAs in HBEC EVs.
    Figure Legend Snippet: HBEC EVs cargo has a unique miRNA profile. A Schematic protocol for the mass spectrometry (MS) and miRNA-seq approach to characterizing EV cargo. EVs were isolated from HBEC and BEAS-2B for MS analysis and miRNA-seq analysis. B Summary of the 30 most abundant miRNAs in HBEC EVs. Data represent read counts for each miRNA species in the miRNA-seq analysis. n= 2. C Relative expression of the 30 most abundant miRNA species in the miRNA microarray dataset. Bars represent the log 2 fold-change in expression of each miRNA, with positive fold-change values indicating higher levels in IPF lung than non-disease lung. Error bars are omitted for clarity. The Gene Expression Omnibus (GEO) database GSE32538 contains 106 IPF/usual interstitial pneumonitis (UIP) samples and 50 non-diseased samples. D Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways from DIANA-mirPath v3.0 analysis, based on the 30 most abundant miRNAs in HBEC EVs. E Gene Ontology from DIANA-mirPath v3.0 analysis, based on the 30 most abundant miRNAs in HBEC EVs.

    Techniques Used: Mass Spectrometry, Isolation, Expressing, Microarray

    miRNA cargo in HBEC EVs attenuates TGF-β-induced myofibroblast differentiation via suppression of WNT5A and WNT10B expression . A Ingenuity Pathway Analysis (IPA) of upstream regulators of WNT ligand genes in LFs treated for 48 h with HBEC EVs. B qRT-PCR analysis of WNT1, WNT3A, WNT5A and WNT10B mRNAs in LFs treated for 24h with HBEC EVs (10 μg/ml, n=3). * P
    Figure Legend Snippet: miRNA cargo in HBEC EVs attenuates TGF-β-induced myofibroblast differentiation via suppression of WNT5A and WNT10B expression . A Ingenuity Pathway Analysis (IPA) of upstream regulators of WNT ligand genes in LFs treated for 48 h with HBEC EVs. B qRT-PCR analysis of WNT1, WNT3A, WNT5A and WNT10B mRNAs in LFs treated for 24h with HBEC EVs (10 μg/ml, n=3). * P

    Techniques Used: Expressing, Indirect Immunoperoxidase Assay, Quantitative RT-PCR

    Related Articles

    RNA Sequencing Assay:

    Article Title: Multisite Evaluation of Next-Generation Methods for Small RNA Quantification
    Article Snippet: Typical smRNA library preparation for Illumina sequencing is based on sequential ligation of oligonucleotides to the 5′ and 3′ ends of the smRNAs. .. Most of the kits tested, including Illumina TruSeq Small RNA Library Prep Kit, Lexogen Small RNA-Seq Library Prep Kit, New England Biolabs NEBNext Small RNA Library Prep Set, PerkinElmer (formerly Bioo Scientific) NextFlex Small RNA-Seq Kit v.3, Qiagen QIAseq miRNA Library Kit, and Trilink CleanTag Small RNA Library Prep Kit all use variants of this methodology. .. The Takara SMARTer smRNA Kit and the Diagenode CATS Small RNA-Seq Kit both begin by polyadenylation of the 3′ nucleotide of the smRNA followed by reverse transcription and template switching.

    Article Title: Evaluation of methodologies for microRNA biomarker detection by next generation sequencing
    Article Snippet: .. In this study, we have benchmarked four commercial kits intended for Illumina sequencing: Nextflex Small RNA-Seq Kit v3, Bioo Scientific (BSC); SMARTer smRNA-Seq Kit, Clontech/Takara (CLT); NEBNext Small RNA Library Prep Set, New England Biolabs (NEB) and QIAseq miRNA Library Kit, Qiagen (QIA). .. All methodologies can generate miRNA sequencing libraries from low input amounts (1–100 ng) and are therefore suitable to process RNA derived from biofluids.

    other:

    Article Title: Multicenter Evaluation of Independent High-Throughput and RT-qPCR Technologies for the Development of Analytical Workflows for Circulating miRNA Analysis
    Article Snippet: The following are available online at https://www.mdpi.com/2072-6694/12/5/1166/s1 , Figure S1: Mapping distribution of different small RNAs measured with the QIAseq miRNA library kit.

    Sequencing:

    Article Title: Evaluation of methodologies for microRNA biomarker detection by next generation sequencing
    Article Snippet: .. In this study, we have benchmarked four commercial kits intended for Illumina sequencing: Nextflex Small RNA-Seq Kit v3, Bioo Scientific (BSC); SMARTer smRNA-Seq Kit, Clontech/Takara (CLT); NEBNext Small RNA Library Prep Set, New England Biolabs (NEB) and QIAseq miRNA Library Kit, Qiagen (QIA). .. All methodologies can generate miRNA sequencing libraries from low input amounts (1–100 ng) and are therefore suitable to process RNA derived from biofluids.

    Article Title: Human bronchial epithelial cell-derived extracellular vesicle therapy for pulmonary fibrosis via inhibition of TGF-β-WNT crosstalk
    Article Snippet: Next generation sequencing and bioinformaticsRNA quantity and quality were determined using a NanoDrop ND-1000 spectrophotometer (Thermo Fisher Scientific Inc.) and an Agilent Bioanalyzer (Agilent Technologies). .. To detect miRNAs present in EVs for next-generation sequencing, the sequencing library was prepared using the QIAseq miRNA Library Kit (Qiagen, 331502), which tags each individual miRNA molecule with a unique molecular index (UMI). .. This is used in data analysis to correct for library amplification bias, followed by amplification of each miRNA via cDNA synthesis/PCR.

    Next-Generation Sequencing:

    Article Title: Human bronchial epithelial cell-derived extracellular vesicle therapy for pulmonary fibrosis via inhibition of TGF-β-WNT crosstalk
    Article Snippet: Next generation sequencing and bioinformaticsRNA quantity and quality were determined using a NanoDrop ND-1000 spectrophotometer (Thermo Fisher Scientific Inc.) and an Agilent Bioanalyzer (Agilent Technologies). .. To detect miRNAs present in EVs for next-generation sequencing, the sequencing library was prepared using the QIAseq miRNA Library Kit (Qiagen, 331502), which tags each individual miRNA molecule with a unique molecular index (UMI). .. This is used in data analysis to correct for library amplification bias, followed by amplification of each miRNA via cDNA synthesis/PCR.

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    Qiagen qiaseq mirna library kit
    Overview of the small RNA sequencing workflow, compared methodologies for library preparation and computational analysis pipeline. (a) Schematic illustration of small RNA sequencing (sRNA-seq) library preparation. With exception of the SMARTer smRNA-Seq kit, all kits employed a ligation-based approach to attach 3′ and 5′ adapter to the <t>miRNA.</t> In the <t>QIAseq</t> protocol, a unique barcode is attached at the RT stage (orange dotted line), so-called unique molecular indices (UMI). Illumina adapter (purple and blue) and sequencing index (light purple) for multiplexing are added during the PCR amplification. Several steps of the protocol may introduce bias to the sequencing libraries as indicated by the yellow warning triangles. (b) Table summary of the key features of the four assessed sRNA-seq kits. (c) Small RNA sequencing data analysis pipeline. In the case of QIAseq, UMIs were trimmed alongside the adapters to allow side-by-side comparison by a single computational pipeline. Abbreviations: Nextflex Small RNA-Seq Kit v3 (Nextflex), NEBNext Small RNA Library Prep Set (NEBNext) and QIAseq miRNA Library Kit (QIAseq).
    Qiaseq Mirna Library Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qiaseq mirna library kit/product/Qiagen
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    qiaseq mirna library kit - by Bioz Stars, 2021-03
    99/100 stars
      Buy from Supplier

    Image Search Results


    Overview of the small RNA sequencing workflow, compared methodologies for library preparation and computational analysis pipeline. (a) Schematic illustration of small RNA sequencing (sRNA-seq) library preparation. With exception of the SMARTer smRNA-Seq kit, all kits employed a ligation-based approach to attach 3′ and 5′ adapter to the miRNA. In the QIAseq protocol, a unique barcode is attached at the RT stage (orange dotted line), so-called unique molecular indices (UMI). Illumina adapter (purple and blue) and sequencing index (light purple) for multiplexing are added during the PCR amplification. Several steps of the protocol may introduce bias to the sequencing libraries as indicated by the yellow warning triangles. (b) Table summary of the key features of the four assessed sRNA-seq kits. (c) Small RNA sequencing data analysis pipeline. In the case of QIAseq, UMIs were trimmed alongside the adapters to allow side-by-side comparison by a single computational pipeline. Abbreviations: Nextflex Small RNA-Seq Kit v3 (Nextflex), NEBNext Small RNA Library Prep Set (NEBNext) and QIAseq miRNA Library Kit (QIAseq).

    Journal: RNA Biology

    Article Title: Evaluation of methodologies for microRNA biomarker detection by next generation sequencing

    doi: 10.1080/15476286.2018.1514236

    Figure Lengend Snippet: Overview of the small RNA sequencing workflow, compared methodologies for library preparation and computational analysis pipeline. (a) Schematic illustration of small RNA sequencing (sRNA-seq) library preparation. With exception of the SMARTer smRNA-Seq kit, all kits employed a ligation-based approach to attach 3′ and 5′ adapter to the miRNA. In the QIAseq protocol, a unique barcode is attached at the RT stage (orange dotted line), so-called unique molecular indices (UMI). Illumina adapter (purple and blue) and sequencing index (light purple) for multiplexing are added during the PCR amplification. Several steps of the protocol may introduce bias to the sequencing libraries as indicated by the yellow warning triangles. (b) Table summary of the key features of the four assessed sRNA-seq kits. (c) Small RNA sequencing data analysis pipeline. In the case of QIAseq, UMIs were trimmed alongside the adapters to allow side-by-side comparison by a single computational pipeline. Abbreviations: Nextflex Small RNA-Seq Kit v3 (Nextflex), NEBNext Small RNA Library Prep Set (NEBNext) and QIAseq miRNA Library Kit (QIAseq).

    Article Snippet: In this study, we have benchmarked four commercial kits intended for Illumina sequencing: Nextflex Small RNA-Seq Kit v3, Bioo Scientific (BSC); SMARTer smRNA-Seq Kit, Clontech/Takara (CLT); NEBNext Small RNA Library Prep Set, New England Biolabs (NEB) and QIAseq miRNA Library Kit, Qiagen (QIA).

    Techniques: RNA Sequencing Assay, Ligation, Sequencing, Multiplexing, Polymerase Chain Reaction, Amplification, Introduce

    QIAseq reveals higher read counts in healthy individuals and NSCLC patients compared to hybridization platforms. Box plot analysis showing comparison of miRNA counts between QIAseq, Toray 3D and nCounter in cfmiRNA and EVmiRNA fractions. The horizontal line in each box represents the median; the whiskers indicate the range; statistical analysis was performed using one-way ANOVA where; ** p

    Journal: Cancers

    Article Title: Multicenter Evaluation of Independent High-Throughput and RT-qPCR Technologies for the Development of Analytical Workflows for Circulating miRNA Analysis

    doi: 10.3390/cancers12051166

    Figure Lengend Snippet: QIAseq reveals higher read counts in healthy individuals and NSCLC patients compared to hybridization platforms. Box plot analysis showing comparison of miRNA counts between QIAseq, Toray 3D and nCounter in cfmiRNA and EVmiRNA fractions. The horizontal line in each box represents the median; the whiskers indicate the range; statistical analysis was performed using one-way ANOVA where; ** p

    Article Snippet: QIAseq miRNA Library KitNGS libraries were prepared from 5 µL aliquots using the QIAseq miRNA Library kit (QIAGEN).

    Techniques: Hybridization

    miRNA cargo in HBEC EVs attenuates TGF-β-induced epithelial cell senescence via suppression of WNT3A and WNT5A expression . A Representative immunoblot showing the amount of active β-catenin and β-actin in HBECs treated for 48 h with PBS or HBEC EVs (10 μg/ml) in the presence or absence of TGF-β1 (2 ng/ml). B qRT-PCR analysis of WNT1, WNT3A, WNT5A and WNT10B mRNAs in HBECs treated for 24 h with HBEC EVs (10 μg/ml, n=3). * P

    Journal: bioRxiv

    Article Title: Human bronchial epithelial cell-derived extracellular vesicle therapy for pulmonary fibrosis via inhibition of TGF-β-WNT crosstalk

    doi: 10.1101/2020.10.22.349761

    Figure Lengend Snippet: miRNA cargo in HBEC EVs attenuates TGF-β-induced epithelial cell senescence via suppression of WNT3A and WNT5A expression . A Representative immunoblot showing the amount of active β-catenin and β-actin in HBECs treated for 48 h with PBS or HBEC EVs (10 μg/ml) in the presence or absence of TGF-β1 (2 ng/ml). B qRT-PCR analysis of WNT1, WNT3A, WNT5A and WNT10B mRNAs in HBECs treated for 24 h with HBEC EVs (10 μg/ml, n=3). * P

    Article Snippet: To detect miRNAs present in EVs for next-generation sequencing, the sequencing library was prepared using the QIAseq miRNA Library Kit (Qiagen, 331502), which tags each individual miRNA molecule with a unique molecular index (UMI).

    Techniques: Expressing, Quantitative RT-PCR

    HBEC EVs cargo has a unique miRNA profile. A Schematic protocol for the mass spectrometry (MS) and miRNA-seq approach to characterizing EV cargo. EVs were isolated from HBEC and BEAS-2B for MS analysis and miRNA-seq analysis. B Summary of the 30 most abundant miRNAs in HBEC EVs. Data represent read counts for each miRNA species in the miRNA-seq analysis. n= 2. C Relative expression of the 30 most abundant miRNA species in the miRNA microarray dataset. Bars represent the log 2 fold-change in expression of each miRNA, with positive fold-change values indicating higher levels in IPF lung than non-disease lung. Error bars are omitted for clarity. The Gene Expression Omnibus (GEO) database GSE32538 contains 106 IPF/usual interstitial pneumonitis (UIP) samples and 50 non-diseased samples. D Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways from DIANA-mirPath v3.0 analysis, based on the 30 most abundant miRNAs in HBEC EVs. E Gene Ontology from DIANA-mirPath v3.0 analysis, based on the 30 most abundant miRNAs in HBEC EVs.

    Journal: bioRxiv

    Article Title: Human bronchial epithelial cell-derived extracellular vesicle therapy for pulmonary fibrosis via inhibition of TGF-β-WNT crosstalk

    doi: 10.1101/2020.10.22.349761

    Figure Lengend Snippet: HBEC EVs cargo has a unique miRNA profile. A Schematic protocol for the mass spectrometry (MS) and miRNA-seq approach to characterizing EV cargo. EVs were isolated from HBEC and BEAS-2B for MS analysis and miRNA-seq analysis. B Summary of the 30 most abundant miRNAs in HBEC EVs. Data represent read counts for each miRNA species in the miRNA-seq analysis. n= 2. C Relative expression of the 30 most abundant miRNA species in the miRNA microarray dataset. Bars represent the log 2 fold-change in expression of each miRNA, with positive fold-change values indicating higher levels in IPF lung than non-disease lung. Error bars are omitted for clarity. The Gene Expression Omnibus (GEO) database GSE32538 contains 106 IPF/usual interstitial pneumonitis (UIP) samples and 50 non-diseased samples. D Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways from DIANA-mirPath v3.0 analysis, based on the 30 most abundant miRNAs in HBEC EVs. E Gene Ontology from DIANA-mirPath v3.0 analysis, based on the 30 most abundant miRNAs in HBEC EVs.

    Article Snippet: To detect miRNAs present in EVs for next-generation sequencing, the sequencing library was prepared using the QIAseq miRNA Library Kit (Qiagen, 331502), which tags each individual miRNA molecule with a unique molecular index (UMI).

    Techniques: Mass Spectrometry, Isolation, Expressing, Microarray

    miRNA cargo in HBEC EVs attenuates TGF-β-induced myofibroblast differentiation via suppression of WNT5A and WNT10B expression . A Ingenuity Pathway Analysis (IPA) of upstream regulators of WNT ligand genes in LFs treated for 48 h with HBEC EVs. B qRT-PCR analysis of WNT1, WNT3A, WNT5A and WNT10B mRNAs in LFs treated for 24h with HBEC EVs (10 μg/ml, n=3). * P

    Journal: bioRxiv

    Article Title: Human bronchial epithelial cell-derived extracellular vesicle therapy for pulmonary fibrosis via inhibition of TGF-β-WNT crosstalk

    doi: 10.1101/2020.10.22.349761

    Figure Lengend Snippet: miRNA cargo in HBEC EVs attenuates TGF-β-induced myofibroblast differentiation via suppression of WNT5A and WNT10B expression . A Ingenuity Pathway Analysis (IPA) of upstream regulators of WNT ligand genes in LFs treated for 48 h with HBEC EVs. B qRT-PCR analysis of WNT1, WNT3A, WNT5A and WNT10B mRNAs in LFs treated for 24h with HBEC EVs (10 μg/ml, n=3). * P

    Article Snippet: To detect miRNAs present in EVs for next-generation sequencing, the sequencing library was prepared using the QIAseq miRNA Library Kit (Qiagen, 331502), which tags each individual miRNA molecule with a unique molecular index (UMI).

    Techniques: Expressing, Indirect Immunoperoxidase Assay, Quantitative RT-PCR