qiaseq mirna library kit (Qiagen)
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Name:
QIAseq miRNA Library Kit
Description:
Gel free miRNA Sample to Insight solution for differential expression analysis and novel discovery using next generation sequencing Kit contents For 12 sequencing prep reactions 3 ligation 5 ligation reverse transcription cDNA cleanup library amplification and library cleanup reagents quality control primers Benefits Gel free miRNA sequencing library prep from as little as 1 ng of total RNA Elimination of adapter dimers and unwanted RNA species resulting in the highest fidelity and most efficient data Bead based method to remove adapter dimers and unwanted RNA species Integrated Unique Molecular Indices UMIs enable quantification of individual miRNA molecules Primary read mapping and differential expression analysis via the GeneGlobe Data Analysis Cen
Catalog Number:
331502
Price:
1120
Category:
QIAseq miRNA Library Kit
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Structured Review
Qiagen
qiaseq mirna library kit
Gel free miRNA Sample to Insight solution for differential expression analysis and novel discovery using next generation sequencing Kit contents For 12 sequencing prep reactions 3 ligation 5 ligation reverse transcription cDNA cleanup library amplification and library cleanup reagents quality control primers Benefits Gel free miRNA sequencing library prep from as little as 1 ng of total RNA Elimination of adapter dimers and unwanted RNA species resulting in the highest fidelity and most efficient data Bead based method to remove adapter dimers and unwanted RNA species Integrated Unique Molecular Indices UMIs enable quantification of individual miRNA molecules Primary read mapping and differential expression analysis via the GeneGlobe Data Analysis Cen
https://www.bioz.com/result/qiaseq mirna library kit/product/Qiagen
Average 99 stars, based on 7 article reviews
Price from $9.99 to $1999.99
Gel free miRNA Sample to Insight solution for differential expression analysis and novel discovery using next generation sequencing Kit contents For 12 sequencing prep reactions 3 ligation 5 ligation reverse transcription cDNA cleanup library amplification and library cleanup reagents quality control primers Benefits Gel free miRNA sequencing library prep from as little as 1 ng of total RNA Elimination of adapter dimers and unwanted RNA species resulting in the highest fidelity and most efficient data Bead based method to remove adapter dimers and unwanted RNA species Integrated Unique Molecular Indices UMIs enable quantification of individual miRNA molecules Primary read mapping and differential expression analysis via the GeneGlobe Data Analysis Cen
https://www.bioz.com/result/qiaseq mirna library kit/product/Qiagen
Average 99 stars, based on 7 article reviews
Price from $9.99 to $1999.99
qiaseq mirna library kit - by Bioz Stars,
2020-04
99/100 stars
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Images
1) Product Images from "Evaluation of methodologies for microRNA biomarker detection by next generation sequencing"
Article Title: Evaluation of methodologies for microRNA biomarker detection by next generation sequencing
Journal: RNA Biology
doi: 10.1080/15476286.2018.1514236
Figure Legend Snippet: Overview of the small RNA sequencing workflow, compared methodologies for library preparation and computational analysis pipeline. (a) Schematic illustration of small RNA sequencing (sRNA-seq) library preparation. With exception of the SMARTer smRNA-Seq kit, all kits employed a ligation-based approach to attach 3′ and 5′ adapter to the miRNA. In the QIAseq protocol, a unique barcode is attached at the RT stage (orange dotted line), so-called unique molecular indices (UMI). Illumina adapter (purple and blue) and sequencing index (light purple) for multiplexing are added during the PCR amplification. Several steps of the protocol may introduce bias to the sequencing libraries as indicated by the yellow warning triangles. (b) Table summary of the key features of the four assessed sRNA-seq kits. (c) Small RNA sequencing data analysis pipeline. In the case of QIAseq, UMIs were trimmed alongside the adapters to allow side-by-side comparison by a single computational pipeline. Abbreviations: Nextflex Small RNA-Seq Kit v3 (Nextflex), NEBNext Small RNA Library Prep Set (NEBNext) and QIAseq miRNA Library Kit (QIAseq).
Techniques Used: RNA Sequencing Assay, Ligation, Sequencing, Multiplexing, Polymerase Chain Reaction, Amplification, Introduce
2) Product Images from "Multisite Evaluation of Next-Generation Methods for Small RNA Quantification"
Article Title: Multisite Evaluation of Next-Generation Methods for Small RNA Quantification
Journal: Journal of Biomolecular Techniques : JBT
doi: 10.7171/jbt.20-3102-001
Figure Legend Snippet: miRNA detection and bias. Percentages of Miltenyi Biotec miRXplore miRNA detected above 5 CPM in MUR ( A ) and in MUR-D ( B ) are shown. Percentages and amplitude of Miltenyi Biotec miRXplore miRNA detected that deviated from the median are shown in MUR ( C ) or MUR-D ( D ). The darkest shade is within 2-fold of the median; the medium shade is 2–10-fold either up or down vs. the expected value; the lightest shade is > 10× either increased or decreased. Percentages of reads increased > 10× from median in MUR ( E ) or MUR-D ( F ). CLO-S and C, Takara Bio (Clontech) SMARTer smRNA-Seq Kit; DIA and D, Diagenode CATS Small RNA-Seq Kit; ILMN and I, Illumina TruSeq Small RNA Library Prep Kit; LEX and L, Lexogen Small RNA-Seq Library Prep Kit; Nano, NanoString nCounter miRNA Expression Assay; NEB and N, New England Biolabs NEBNext Small RNA Library Prep Set; PEB and P, PerkinElmer NextFlex Small RNA-Seq Kit v.3; QIA and Q, Qiagen QIAseq miRNA Library Kit; SOM and S, Somagenics RealSeq-AC miRNA Library Kit; TRI and T, Trilink CleanTag Small RNA Library Prep Kit.
Techniques Used: RNA Sequencing Assay, Expressing
Figure Legend Snippet: Study design and workflow metrics. A ) Schematic of study design is shown. MUR, MUR-D, and HBR were processed using 9 different smRNA profiling methods at 4 sites each. The general methodologies included sequential ligation (Illumina, TriLink, Qiagen, NEB, PerkinElmer, Lexogen), template switching (Takara, Diagenode), circularization (Somagenics), and NanoString probe-based hybridization. B ) Total start-to-finish preparation time for each kit as reported by sites. C ) Mean “ease of use” reported by each site (scale: 1 = uncomfortable, 5 = comfortable). D ) Library preparation success rates. Light color blocks are successfully produced libraries. Dark colors are failed libraries. CLO and CLO-S, Takara Bio (Clontech) SMARTer smRNA-Seq Kit; DIA, Diagenode CATS Small RNA-Seq Kit; ILL and ILMN, Illumina TruSeq Small RNA Library Prep Kit; LEX, Lexogen Small RNA-Seq Library Prep Kit; NANO, NanoString nCounter miRNA Expression Assay; NEB, New England Biolabs NEBNext Small RNA Library Prep Set; PEB, PerkinElmer NextFlex Small RNA-Seq Kit v.3; QIA, Qiagen QIAseq miRNA Library Kit; SOM, Somagenics RealSeq-AC miRNA Library Kit; TRI, Trilink CleanTag Small RNA Library Prep Kit.
Techniques Used: Ligation, Hybridization, Produced, RNA Sequencing Assay, Expressing
Figure Legend Snippet: Relative expression of miRNAs. A ) Correlation scatter plots of miRNAs detected in MUR and MUR-D for the QIA method, with miRNAs scored in log2 mapped reads per million. B ) Correlation scatter plot of miRNAs detected in MUR-D using LEX vs. ILL kits, with miRNAs scored in log2 mapped reads per million. C ) Unsupervised hierarchical clustering heatmap of log2 transformed CPM across all methods. C (prep kit), Takara Bio (Clontech) SMARTer smRNA-Seq Kit; C (prep type), circularization; D, Diagenode CATS Small RNA-Seq Kit; ILL and I, Illumina TruSeq Small RNA Library Prep Kit; L (prep type), sequential ligation; LEX and L (prep kit), Lexogen Small RNA-Seq Library Prep Kit; N, New England Biolabs NEBNext Small RNA Library Prep Set; Nano, NanoString nCounter miRNA Expression Assay; NO, No size selection; P, PerkinElmer NextFlex Small RNA-Seq Kit v.3; QIA and Q, Qiagen QIAseq miRNA Library Kit; S, Somagenics RealSeq-AC miRNA Library Kit; SAGE, pippin prep; SPRI, solid phase reversible immobilization; T (prep kit), Trilink CleanTag Small Library Prep Kit; T (prep type), template switching.
Techniques Used: Expressing, Transformation Assay, RNA Sequencing Assay, Ligation, Selection
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In this study, we have benchmarked four commercial kits intended for Illumina sequencing: Nextflex Small RNA-Seq Kit v3, Bioo Scientific (BSC); SMARTer smRNA-Seq Kit, Clontech/Takara (CLT); NEBNext Small RNA Library Prep Set, New England Biolabs (NEB) and Article Title: HNRNPA2/B1 is upregulated in endocrine-resistant LCC9 breast cancer cells and alters the miRNA transcriptome when overexpressed in MCF-7 cells Article Snippet: Libraries were prepared using Construct:Article Title: Systematic Analysis of Transcriptomic Profile of the Effects of Low Dose Atropine Treatment on Scleral Fibroblasts using Next-Generation Sequencing and Bioinformatics Article Snippet: Samples were prepared with the Microarray:Article Title: Multisite Evaluation of Next-Generation Methods for Small RNA Quantification Article Snippet: RNA from the HBR (AM7962; Thermo Fisher Scientific), a commercially available sample from the 2006 Microarray Quality Control study, was used as a second standard because it is commonly used to characterize miRNA chemistries by other studies and provides a highly complex normal sample. .. Most of the kits tested, including Illumina TruSeq Small RNA Library Prep Kit, Lexogen Small RNA-Seq Library Prep Kit, New England Biolabs NEBNext Small RNA Library Prep Set, PerkinElmer (formerly Bioo Scientific) NextFlex Small RNA-Seq Kit v.3, Qiagen Significance Assay:Article Title: Immunophenotyping and transcriptional profiling of in vitro cultured human adipose tissue derived stem cells Article Snippet: Transcripts that showed significant and consistent differences between conditions in our dataset (FDR corrected p-value, q-value < 0.05) were subjected to further interpretation with Ingenuity Pathway Analysis. .. 2 combination (Supplementary Table ). miRNA-seq was performed for the same set of five biological replicate experiments using Expressing:Article Title: PIWI proteins contribute to apoptosis during the UPR in human airway epithelial cells Article Snippet: Small RNA sequencing libraries were prepared using Article Title: Systematic Analysis of Transcriptomic Profile of the Effects of Low Dose Atropine Treatment on Scleral Fibroblasts using Next-Generation Sequencing and Bioinformatics Article Snippet: Paragraph title: Next-Generation Sequencing (NGS) for miRNA and mRNA Expression Profiling ... 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Library preparation and sequencing Libraries were prepared from 5 μl of miRNeasy RNA using NEXTflex® Small RNA Sequencing Kit v3 for Illumina® Platforms (Bioo Scientific) and Article Title: Transforming Growth Factor-β1 Selectively Recruits microRNAs to the RNA-Induced Silencing Complex and Degrades CFTR mRNA under Permissive Conditions in Human Bronchial Epithelial Cells Article Snippet: F508del-CFTR Modulates TGF-β1-Induced Changes in miRNA Landscape We used small RNA-seq to examine TGF-β1 effects on the entire miRNA landscape. .. Cells were treated with TGF-β1 or vehicle for 24 h, total RNA was isolated and used with Isolation:Article Title: A comparison of RNA extraction and sequencing protocols for detection of small RNAs in plasma Article Snippet: Article Title: PIWI proteins contribute to apoptosis during the UPR in human airway epithelial cells Article Snippet: Next generation small RNAs sequencing analyses 16HBE14o- cells were used for the RNA isolation and analyses. .. Small RNA sequencing libraries were prepared using Article Title: HNRNPA2/B1 is upregulated in endocrine-resistant LCC9 breast cancer cells and alters the miRNA transcriptome when overexpressed in MCF-7 cells Article Snippet: For miRNA-seq miRNA was isolated from six separate, biological replicate experiments for each sample group (Control, HNRNPA2/B1 48 h, HNRNPA2/B172 h) using the miRNeasy mini kit from Qiagen (Hilden, Germany) following the manufacturer’s directions. .. Libraries were prepared using Article Title: Transforming Growth Factor-β1 Selectively Recruits microRNAs to the RNA-Induced Silencing Complex and Degrades CFTR mRNA under Permissive Conditions in Human Bronchial Epithelial Cells Article Snippet: .. Cells were treated with TGF-β1 or vehicle for 24 h, total RNA was isolated and used with RNA Extraction:Article Title: A comparison of RNA extraction and sequencing protocols for detection of small RNAs in plasma Article Snippet: Purification:Article Title: Inheritance of a Phenotypically Neutral Epimutation Evokes Gene Silencing in Later Generations Article Snippet: Total RNA from exponentially growing cultures was extracted with the MasterPure Yeast RNA Purification Kit (Epicenter). .. Small RNA libraries were prepared with the Article Title: Systematic Analysis of Transcriptomic Profile of the Effects of Low Dose Atropine Treatment on Scleral Fibroblasts using Next-Generation Sequencing and Bioinformatics Article Snippet: Purified RNA was quantified at an optical density of 260nm with a ND-1000 spectrophotometer (Nanodrop Technologies, Wilmington, DE, USA). .. Samples were prepared with the Polymerase Chain Reaction:Article Title: A comparison of RNA extraction and sequencing protocols for detection of small RNAs in plasma Article Snippet: Article Title: Evaluation of methodologies for microRNA biomarker detection by next generation sequencing Article Snippet: As such, certain adapter-miRNA pairs or sequence compositions may be favoured over others during ligation and PCR amplification, and thereby result in over- or underrepresentation of these miRNAs in the sequencing library [ , ]. .. 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In this study, we have benchmarked four commercial kits intended for Illumina sequencing: Nextflex Small RNA-Seq Kit v3, Bioo Scientific (BSC); SMARTer smRNA-Seq Kit, Clontech/Takara (CLT); NEBNext Small RNA Library Prep Set, New England Biolabs (NEB) and Next-Generation Sequencing:Article Title: Cigarette Smoke Condensate Exposure Changes RNA Content of Extracellular Vesicles Released from Small Airway Epithelial Cells Article Snippet: Paragraph title: 2.5. Extraction of EVs RNA and Next Generation Sequencing (NGS) ... Small RNA libraries were made using the Article Title: HNRNPA2/B1 is upregulated in endocrine-resistant LCC9 breast cancer cells and alters the miRNA transcriptome when overexpressed in MCF-7 cells Article Snippet: .. Libraries were prepared using Article Title: Systematic Analysis of Transcriptomic Profile of the Effects of Low Dose Atropine Treatment on Scleral Fibroblasts using Next-Generation Sequencing and Bioinformatics Article Snippet: Paragraph title: Next-Generation Sequencing (NGS) for miRNA and mRNA Expression Profiling ... Samples were prepared with the Spectrophotometry:Article Title: Systematic Analysis of Transcriptomic Profile of the Effects of Low Dose Atropine Treatment on Scleral Fibroblasts using Next-Generation Sequencing and Bioinformatics Article Snippet: Purified RNA was quantified at an optical density of 260nm with a ND-1000 spectrophotometer (Nanodrop Technologies, Wilmington, DE, USA). .. Samples were prepared with the Concentration Assay:Article Title: A comparison of RNA extraction and sequencing protocols for detection of small RNAs in plasma Article Snippet: Library preparation and sequencing Libraries were prepared from 5 μl of miRNeasy RNA using NEXTflex® Small RNA Sequencing Kit v3 for Illumina® Platforms (Bioo Scientific) and Variant Assay:Article Title: Transforming Growth Factor-β1 Selectively Recruits microRNAs to the RNA-Induced Silencing Complex and Degrades CFTR mRNA under Permissive Conditions in Human Bronchial Epithelial Cells Article Snippet: Cells were treated with TGF-β1 or vehicle for 24 h, total RNA was isolated and used with |