qiaseq mirna library kit (Qiagen)
Qiagen is a verified supplier 
Qiagen manufactures this product 
90
|
Name:
QIAseq miRNA Library Kit
Description:
Gel free miRNA Sample to Insight solution for differential expression analysis and novel discovery using next generation sequencing Kit contents For 12 sequencing prep reactions 3 ligation 5 ligation reverse transcription cDNA cleanup library amplification and library cleanup reagents quality control primers Benefits Gel free miRNA sequencing library prep from as little as 1 ng of total RNA Elimination of adapter dimers and unwanted RNA species resulting in the highest fidelity and most efficient data Bead based method to remove adapter dimers and unwanted RNA species Integrated Unique Molecular Indices UMIs enable quantification of individual miRNA molecules Primary read mapping and differential expression analysis via the GeneGlobe Data Analysis Cen
Catalog Number:
331502
Price:
1120
Category:
QIAseq miRNA Library Kit
|
Buy from Supplier |
Structured Review
Qiagen
qiaseq mirna library kit
Gel free miRNA Sample to Insight solution for differential expression analysis and novel discovery using next generation sequencing Kit contents For 12 sequencing prep reactions 3 ligation 5 ligation reverse transcription cDNA cleanup library amplification and library cleanup reagents quality control primers Benefits Gel free miRNA sequencing library prep from as little as 1 ng of total RNA Elimination of adapter dimers and unwanted RNA species resulting in the highest fidelity and most efficient data Bead based method to remove adapter dimers and unwanted RNA species Integrated Unique Molecular Indices UMIs enable quantification of individual miRNA molecules Primary read mapping and differential expression analysis via the GeneGlobe Data Analysis Cen
https://www.bioz.com/result/qiaseq mirna library kit/product/Qiagen
Average 90 stars, based on 18 article reviews
Price from $9.99 to $1999.99
Gel free miRNA Sample to Insight solution for differential expression analysis and novel discovery using next generation sequencing Kit contents For 12 sequencing prep reactions 3 ligation 5 ligation reverse transcription cDNA cleanup library amplification and library cleanup reagents quality control primers Benefits Gel free miRNA sequencing library prep from as little as 1 ng of total RNA Elimination of adapter dimers and unwanted RNA species resulting in the highest fidelity and most efficient data Bead based method to remove adapter dimers and unwanted RNA species Integrated Unique Molecular Indices UMIs enable quantification of individual miRNA molecules Primary read mapping and differential expression analysis via the GeneGlobe Data Analysis Cen
https://www.bioz.com/result/qiaseq mirna library kit/product/Qiagen
Average 90 stars, based on 18 article reviews
Price from $9.99 to $1999.99
qiaseq mirna library kit - by Bioz Stars,
2020-01
90/100 stars
Related Products / Commonly Used Together
Images
Related Articles
Amplification:Article Title: Bulky DNA adducts, microRNA profiles, and lipid biomarkers in Norwegian tunnel finishing workers occupationally exposed to diesel exhaust Article Snippet: Small RNA sequencing (sRNA-seq) Libraries for sRNA-seq were prepared using Article Title: Evaluation of methodologies for microRNA biomarker detection by next generation sequencing Article Snippet: As such, certain adapter-miRNA pairs or sequence compositions may be favoured over others during ligation and PCR amplification, and thereby result in over- or underrepresentation of these miRNAs in the sequencing library [ , ]. .. In this study, we have benchmarked four commercial kits intended for Illumina sequencing: Nextflex Small RNA-Seq Kit v3, Bioo Scientific (BSC); SMARTer smRNA-Seq Kit, Clontech/Takara (CLT); NEBNext Small RNA Library Prep Set, New England Biolabs (NEB) and Article Title: Mesenchymal Stem Cells Reduce Corneal Fibrosis and Inflammation via Extracellular Vesicle‐Mediated Delivery of miRNA Article Snippet: As a control for amplification or losses during the assay process, a nonmammalian miRNA “spike‐in” (ath‐miR159a) was added to a known concentration to the RNA samples before reverse transcription. miRNA content of CSSC‐derived EVs was determined for 800 human miRNA species by screening RNA isolated from EVs produced by CSSC isolated from eight different donors, from human corneal fibroblasts, and from HEK293T cells using a Nanostring Array (Nanostring, Inc., CSO‐MIR3‐12). .. A strand‐specific small RNA library was produced from EV RNA using the Article Title: Dose-dependent effects of chronic alcohol drinking on peripheral immune responses Article Snippet: Small RNA library preparation Small RNA libraries were generated from total RNA as starting material using Article Title: HNRNPA2/B1 is upregulated in endocrine-resistant LCC9 breast cancer cells and alters the miRNA transcriptome when overexpressed in MCF-7 cells Article Snippet: Libraries were prepared using Construct:Article Title: Systematic Analysis of Transcriptomic Profile of the Effects of Low Dose Atropine Treatment on Scleral Fibroblasts using Next-Generation Sequencing and Bioinformatics Article Snippet: Samples were prepared with the Incubation:Article Title: eNOS expression and NO release during hypoxia is inhibited by miR-200b in human endothelial cells Article Snippet: Next generation sequencing analyses of miRNAs HUVECs at passage 3 were incubated under normoxic or hypoxic conditions for 16 h, and subsequently used for the RNA isolation and analyses. .. MiRNA sequencing libraries were prepared using Significance Assay:Article Title: Immunophenotyping and transcriptional profiling of in vitro cultured human adipose tissue derived stem cells Article Snippet: Transcripts that showed significant and consistent differences between conditions in our dataset (FDR corrected p-value, q-value < 0.05) were subjected to further interpretation with Ingenuity Pathway Analysis. .. 2 combination (Supplementary Table ). miRNA-seq was performed for the same set of five biological replicate experiments using Expressing:Article Title: PIWI proteins contribute to apoptosis during the UPR in human airway epithelial cells Article Snippet: Small RNA sequencing libraries were prepared using Article Title: eNOS expression and NO release during hypoxia is inhibited by miR-200b in human endothelial cells Article Snippet: MiRNA sequencing libraries were prepared using Article Title: Systematic Analysis of Transcriptomic Profile of the Effects of Low Dose Atropine Treatment on Scleral Fibroblasts using Next-Generation Sequencing and Bioinformatics Article Snippet: Paragraph title: Next-Generation Sequencing (NGS) for miRNA and mRNA Expression Profiling ... Samples were prepared with the Modification:Article Title: A comparison of RNA extraction and sequencing protocols for detection of small RNAs in plasma Article Snippet: CleanTag™ Small RNA Library Prep Kit (TriLink) uses modified adapters to reduce adapter dimer formation from low inputs of RNA [ ]. .. Derivative Assay:Article Title: Evaluation of methodologies for microRNA biomarker detection by next generation sequencing Article Snippet: In this study, we have benchmarked four commercial kits intended for Illumina sequencing: Nextflex Small RNA-Seq Kit v3, Bioo Scientific (BSC); SMARTer smRNA-Seq Kit, Clontech/Takara (CLT); NEBNext Small RNA Library Prep Set, New England Biolabs (NEB) and Ligation:Article Title: Evaluation of methodologies for microRNA biomarker detection by next generation sequencing Article Snippet: As such, certain adapter-miRNA pairs or sequence compositions may be favoured over others during ligation and PCR amplification, and thereby result in over- or underrepresentation of these miRNAs in the sequencing library [ , ]. .. In this study, we have benchmarked four commercial kits intended for Illumina sequencing: Nextflex Small RNA-Seq Kit v3, Bioo Scientific (BSC); SMARTer smRNA-Seq Kit, Clontech/Takara (CLT); NEBNext Small RNA Library Prep Set, New England Biolabs (NEB) and Article Title: Systematic Analysis of Transcriptomic Profile of the Effects of Low Dose Atropine Treatment on Scleral Fibroblasts using Next-Generation Sequencing and Bioinformatics Article Snippet: Samples were prepared with the Biomarker Assay:Article Title: A comparison of RNA extraction and sequencing protocols for detection of small RNAs in plasma Article Snippet: Issues particularly pertinent for blood-based biomarker discovery are the ability to work with low miRNA concentrations and reduction of bias. .. Indirect Immunoperoxidase Assay:Article Title: Immunophenotyping and transcriptional profiling of in vitro cultured human adipose tissue derived stem cells Article Snippet: Differentially expressed genes were grouped in functional pathways using Ingenuity Pathway Analysis (IPA Spring Release 2017, Redwood City, USA) on gene level using RefSeq annotation. .. 2 combination (Supplementary Table ). miRNA-seq was performed for the same set of five biological replicate experiments using Generated:Article Title: Dose-dependent effects of chronic alcohol drinking on peripheral immune responses Article Snippet: .. Small RNA library preparation Small RNA libraries were generated from total RNA as starting material using Sequencing:Article Title: A comparison of RNA extraction and sequencing protocols for detection of small RNAs in plasma Article Snippet: NEXTflex® Small RNA Sequencing Kit v3 (Bioo Scientific) uses randomised adapters to reduce sequencing bias and adapter dimer reduction technology to allow low inputs of RNA [ ]. .. Article Title: PIWI proteins contribute to apoptosis during the UPR in human airway epithelial cells Article Snippet: .. Small RNA sequencing libraries were prepared using Article Title: Immunophenotyping and transcriptional profiling of in vitro cultured human adipose tissue derived stem cells Article Snippet: Paragraph title: RNA- and miRNA Sequencing and Bioinformatic Analysis ... 2 combination (Supplementary Table ). miRNA-seq was performed for the same set of five biological replicate experiments using Article Title: Bulky DNA adducts, microRNA profiles, and lipid biomarkers in Norwegian tunnel finishing workers occupationally exposed to diesel exhaust Article Snippet: Small RNA sequencing (sRNA-seq) Libraries for sRNA-seq were prepared using Article Title: Evaluation of methodologies for microRNA biomarker detection by next generation sequencing Article Snippet: .. In this study, we have benchmarked four commercial kits intended for Illumina sequencing: Nextflex Small RNA-Seq Kit v3, Bioo Scientific (BSC); SMARTer smRNA-Seq Kit, Clontech/Takara (CLT); NEBNext Small RNA Library Prep Set, New England Biolabs (NEB) and Article Title: Mesenchymal Stem Cells Reduce Corneal Fibrosis and Inflammation via Extracellular Vesicle‐Mediated Delivery of miRNA Article Snippet: RNA sequence analysis. .. A strand‐specific small RNA library was produced from EV RNA using the Article Title: Exosomes from Endothelial Progenitor Cells Improve the Outcome of a Murine Model of Sepsis Article Snippet: Extracted RNA (100 ng) was used to prepare miRNA-focused NGS libraries using Article Title: A comparison of RNA extraction and sequencing protocols for detection of small RNAs in plasma Article Snippet: .. Library preparation and sequencing Libraries were prepared from 5 μl of miRNeasy RNA using NEXTflex® Small RNA Sequencing Kit v3 for Illumina® Platforms (Bioo Scientific) and Article Title: eNOS expression and NO release during hypoxia is inhibited by miR-200b in human endothelial cells Article Snippet: .. MiRNA sequencing libraries were prepared using Article Title: Systematic Analysis of Transcriptomic Profile of the Effects of Low Dose Atropine Treatment on Scleral Fibroblasts using Next-Generation Sequencing and Bioinformatics Article Snippet: Samples were prepared with the RNA Sequencing Assay:Article Title: A comparison of RNA extraction and sequencing protocols for detection of small RNAs in plasma Article Snippet: NEXTflex® Small RNA Sequencing Kit v3 (Bioo Scientific) uses randomised adapters to reduce sequencing bias and adapter dimer reduction technology to allow low inputs of RNA [ ]. .. Article Title: PIWI proteins contribute to apoptosis during the UPR in human airway epithelial cells Article Snippet: .. Small RNA sequencing libraries were prepared using Article Title: Inheritance of a Phenotypically Neutral Epimutation Evokes Gene Silencing in Later Generations Article Snippet: Paragraph title: Small RNA sequencing and analysis ... Small RNA libraries were prepared with the Article Title: Bulky DNA adducts, microRNA profiles, and lipid biomarkers in Norwegian tunnel finishing workers occupationally exposed to diesel exhaust Article Snippet: .. Small RNA sequencing (sRNA-seq) Libraries for sRNA-seq were prepared using Article Title: Evaluation of methodologies for microRNA biomarker detection by next generation sequencing Article Snippet: .. In this study, we have benchmarked four commercial kits intended for Illumina sequencing: Nextflex Small RNA-Seq Kit v3, Bioo Scientific (BSC); SMARTer smRNA-Seq Kit, Clontech/Takara (CLT); NEBNext Small RNA Library Prep Set, New England Biolabs (NEB) and Article Title: A comparison of RNA extraction and sequencing protocols for detection of small RNAs in plasma Article Snippet: .. Library preparation and sequencing Libraries were prepared from 5 μl of miRNeasy RNA using NEXTflex® Small RNA Sequencing Kit v3 for Illumina® Platforms (Bioo Scientific) and Isolation:Article Title: A comparison of RNA extraction and sequencing protocols for detection of small RNAs in plasma Article Snippet: Article Title: PIWI proteins contribute to apoptosis during the UPR in human airway epithelial cells Article Snippet: Next generation small RNAs sequencing analyses 16HBE14o- cells were used for the RNA isolation and analyses. .. Small RNA sequencing libraries were prepared using Article Title: Mesenchymal Stem Cells Reduce Corneal Fibrosis and Inflammation via Extracellular Vesicle‐Mediated Delivery of miRNA Article Snippet: Paragraph title: miRNA Isolation and Analysis ... A strand‐specific small RNA library was produced from EV RNA using the Article Title: HNRNPA2/B1 is upregulated in endocrine-resistant LCC9 breast cancer cells and alters the miRNA transcriptome when overexpressed in MCF-7 cells Article Snippet: For miRNA-seq miRNA was isolated from six separate, biological replicate experiments for each sample group (Control, HNRNPA2/B1 48 h, HNRNPA2/B172 h) using the miRNeasy mini kit from Qiagen (Hilden, Germany) following the manufacturer’s directions. .. Libraries were prepared using Article Title: eNOS expression and NO release during hypoxia is inhibited by miR-200b in human endothelial cells Article Snippet: Next generation sequencing analyses of miRNAs HUVECs at passage 3 were incubated under normoxic or hypoxic conditions for 16 h, and subsequently used for the RNA isolation and analyses. .. MiRNA sequencing libraries were prepared using RNA Extraction:Article Title: A comparison of RNA extraction and sequencing protocols for detection of small RNAs in plasma Article Snippet: Purification:Article Title: Inheritance of a Phenotypically Neutral Epimutation Evokes Gene Silencing in Later Generations Article Snippet: Total RNA from exponentially growing cultures was extracted with the MasterPure Yeast RNA Purification Kit (Epicenter). .. Small RNA libraries were prepared with the Article Title: Systematic Analysis of Transcriptomic Profile of the Effects of Low Dose Atropine Treatment on Scleral Fibroblasts using Next-Generation Sequencing and Bioinformatics Article Snippet: Purified RNA was quantified at an optical density of 260nm with a ND-1000 spectrophotometer (Nanodrop Technologies, Wilmington, DE, USA). .. Samples were prepared with the Polymerase Chain Reaction:Article Title: A comparison of RNA extraction and sequencing protocols for detection of small RNAs in plasma Article Snippet: Article Title: Evaluation of methodologies for microRNA biomarker detection by next generation sequencing Article Snippet: As such, certain adapter-miRNA pairs or sequence compositions may be favoured over others during ligation and PCR amplification, and thereby result in over- or underrepresentation of these miRNAs in the sequencing library [ , ]. .. In this study, we have benchmarked four commercial kits intended for Illumina sequencing: Nextflex Small RNA-Seq Kit v3, Bioo Scientific (BSC); SMARTer smRNA-Seq Kit, Clontech/Takara (CLT); NEBNext Small RNA Library Prep Set, New England Biolabs (NEB) and Article Title: Dose-dependent effects of chronic alcohol drinking on peripheral immune responses Article Snippet: Small RNA library preparation Small RNA libraries were generated from total RNA as starting material using Software:Article Title: PIWI proteins contribute to apoptosis during the UPR in human airway epithelial cells Article Snippet: Small RNA sequencing libraries were prepared using Article Title: Mesenchymal Stem Cells Reduce Corneal Fibrosis and Inflammation via Extracellular Vesicle‐Mediated Delivery of miRNA Article Snippet: Relative abundance was determined using nSolver software. .. A strand‐specific small RNA library was produced from EV RNA using the Article Title: eNOS expression and NO release during hypoxia is inhibited by miR-200b in human endothelial cells Article Snippet: MiRNA sequencing libraries were prepared using Article Title: Systematic Analysis of Transcriptomic Profile of the Effects of Low Dose Atropine Treatment on Scleral Fibroblasts using Next-Generation Sequencing and Bioinformatics Article Snippet: Samples were prepared with the Functional Assay:Article Title: Immunophenotyping and transcriptional profiling of in vitro cultured human adipose tissue derived stem cells Article Snippet: Differentially expressed genes were grouped in functional pathways using Ingenuity Pathway Analysis (IPA Spring Release 2017, Redwood City, USA) on gene level using RefSeq annotation. .. 2 combination (Supplementary Table ). miRNA-seq was performed for the same set of five biological replicate experiments using Selection:Article Title: Evaluation of methodologies for microRNA biomarker detection by next generation sequencing Article Snippet: In order to generate small RNA sequencing (sRNA-seq) libraries, adapters are ligated to both ends of the miRNA, followed by reverse transcription, template amplification by PCR and size selection of small RNA species. .. In this study, we have benchmarked four commercial kits intended for Illumina sequencing: Nextflex Small RNA-Seq Kit v3, Bioo Scientific (BSC); SMARTer smRNA-Seq Kit, Clontech/Takara (CLT); NEBNext Small RNA Library Prep Set, New England Biolabs (NEB) and Next-Generation Sequencing:Article Title: Dose-dependent effects of chronic alcohol drinking on peripheral immune responses Article Snippet: Small RNA library preparation Small RNA libraries were generated from total RNA as starting material using Article Title: HNRNPA2/B1 is upregulated in endocrine-resistant LCC9 breast cancer cells and alters the miRNA transcriptome when overexpressed in MCF-7 cells Article Snippet: .. Libraries were prepared using Article Title: Exosomes from Endothelial Progenitor Cells Improve the Outcome of a Murine Model of Sepsis Article Snippet: .. Extracted RNA (100 ng) was used to prepare miRNA-focused NGS libraries using Article Title: eNOS expression and NO release during hypoxia is inhibited by miR-200b in human endothelial cells Article Snippet: Paragraph title: Next generation sequencing analyses of miRNAs ... MiRNA sequencing libraries were prepared using Article Title: Systematic Analysis of Transcriptomic Profile of the Effects of Low Dose Atropine Treatment on Scleral Fibroblasts using Next-Generation Sequencing and Bioinformatics Article Snippet: Paragraph title: Next-Generation Sequencing (NGS) for miRNA and mRNA Expression Profiling ... Samples were prepared with the Spectrophotometry:Article Title: Systematic Analysis of Transcriptomic Profile of the Effects of Low Dose Atropine Treatment on Scleral Fibroblasts using Next-Generation Sequencing and Bioinformatics Article Snippet: Purified RNA was quantified at an optical density of 260nm with a ND-1000 spectrophotometer (Nanodrop Technologies, Wilmington, DE, USA). .. Samples were prepared with the Produced:Article Title: Mesenchymal Stem Cells Reduce Corneal Fibrosis and Inflammation via Extracellular Vesicle‐Mediated Delivery of miRNA Article Snippet: .. A strand‐specific small RNA library was produced from EV RNA using the Concentration Assay:Article Title: Mesenchymal Stem Cells Reduce Corneal Fibrosis and Inflammation via Extracellular Vesicle‐Mediated Delivery of miRNA Article Snippet: As a control for amplification or losses during the assay process, a nonmammalian miRNA “spike‐in” (ath‐miR159a) was added to a known concentration to the RNA samples before reverse transcription. miRNA content of CSSC‐derived EVs was determined for 800 human miRNA species by screening RNA isolated from EVs produced by CSSC isolated from eight different donors, from human corneal fibroblasts, and from HEK293T cells using a Nanostring Array (Nanostring, Inc., CSO‐MIR3‐12). .. A strand‐specific small RNA library was produced from EV RNA using the Article Title: A comparison of RNA extraction and sequencing protocols for detection of small RNAs in plasma Article Snippet: Library preparation and sequencing Libraries were prepared from 5 μl of miRNeasy RNA using NEXTflex® Small RNA Sequencing Kit v3 for Illumina® Platforms (Bioo Scientific) and Genomic Sequencing:Article Title: Exosomes from Endothelial Progenitor Cells Improve the Outcome of a Murine Model of Sepsis Article Snippet: Extracted RNA (100 ng) was used to prepare miRNA-focused NGS libraries using |