mirnas (Qiagen)


Name:
QIAseq miRNA Library Kit
Description:
Gel free miRNA Sample to Insight solution for differential expression analysis and novel discovery using next generation sequencing Kit contents For 12 sequencing prep reactions 3 ligation 5 ligation reverse transcription cDNA cleanup library amplification and library cleanup reagents quality control primers Benefits Gel free miRNA sequencing library prep from as little as 1 ng of total RNA Elimination of adapter dimers and unwanted RNA species resulting in the highest fidelity and most efficient data Bead based method to remove adapter dimers and unwanted RNA species Integrated Unique Molecular Indices UMIs enable quantification of individual miRNA molecules Primary read mapping and differential expression analysis via the GeneGlobe Data Analysis Cen
Catalog Number:
331502
Price:
1120
Category:
QIAseq miRNA Library Kit
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Structured Review
Gel free miRNA Sample to Insight solution for differential expression analysis and novel discovery using next generation sequencing Kit contents For 12 sequencing prep reactions 3 ligation 5 ligation reverse transcription cDNA cleanup library amplification and library cleanup reagents quality control primers Benefits Gel free miRNA sequencing library prep from as little as 1 ng of total RNA Elimination of adapter dimers and unwanted RNA species resulting in the highest fidelity and most efficient data Bead based method to remove adapter dimers and unwanted RNA species Integrated Unique Molecular Indices UMIs enable quantification of individual miRNA molecules Primary read mapping and differential expression analysis via the GeneGlobe Data Analysis Cen
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Average 99 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Human bronchial epithelial cell-derived extracellular vesicle therapy for pulmonary fibrosis via inhibition of TGF-β-WNT crosstalk"
Article Title: Human bronchial epithelial cell-derived extracellular vesicle therapy for pulmonary fibrosis via inhibition of TGF-β-WNT crosstalk
Journal: bioRxiv
doi: 10.1101/2020.10.22.349761

Figure Legend Snippet: miRNA cargo in HBEC EVs attenuates TGF-β-induced epithelial cell senescence via suppression of WNT3A and WNT5A expression . A Representative immunoblot showing the amount of active β-catenin and β-actin in HBECs treated for 48 h with PBS or HBEC EVs (10 μg/ml) in the presence or absence of TGF-β1 (2 ng/ml). B qRT-PCR analysis of WNT1, WNT3A, WNT5A and WNT10B mRNAs in HBECs treated for 24 h with HBEC EVs (10 μg/ml, n=3). * P
Techniques Used: Expressing, Quantitative RT-PCR

Figure Legend Snippet: HBEC EVs cargo has a unique miRNA profile. A Schematic protocol for the mass spectrometry (MS) and miRNA-seq approach to characterizing EV cargo. EVs were isolated from HBEC and BEAS-2B for MS analysis and miRNA-seq analysis. B Summary of the 30 most abundant miRNAs in HBEC EVs. Data represent read counts for each miRNA species in the miRNA-seq analysis. n= 2. C Relative expression of the 30 most abundant miRNA species in the miRNA microarray dataset. Bars represent the log 2 fold-change in expression of each miRNA, with positive fold-change values indicating higher levels in IPF lung than non-disease lung. Error bars are omitted for clarity. The Gene Expression Omnibus (GEO) database GSE32538 contains 106 IPF/usual interstitial pneumonitis (UIP) samples and 50 non-diseased samples. D Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways from DIANA-mirPath v3.0 analysis, based on the 30 most abundant miRNAs in HBEC EVs. E Gene Ontology from DIANA-mirPath v3.0 analysis, based on the 30 most abundant miRNAs in HBEC EVs.
Techniques Used: Mass Spectrometry, Isolation, Expressing, Microarray

Figure Legend Snippet: miRNA cargo in HBEC EVs attenuates TGF-β-induced myofibroblast differentiation via suppression of WNT5A and WNT10B expression . A Ingenuity Pathway Analysis (IPA) of upstream regulators of WNT ligand genes in LFs treated for 48 h with HBEC EVs. B qRT-PCR analysis of WNT1, WNT3A, WNT5A and WNT10B mRNAs in LFs treated for 24h with HBEC EVs (10 μg/ml, n=3). * P
Techniques Used: Expressing, Indirect Immunoperoxidase Assay, Quantitative RT-PCR
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