qiaseq mirna library kit  (Qiagen)


Bioz Verified Symbol Qiagen is a verified supplier
Bioz Manufacturer Symbol Qiagen manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90
    Name:
    QIAseq miRNA Library Kit
    Description:
    Gel free miRNA Sample to Insight solution for differential expression analysis and novel discovery using next generation sequencing Kit contents For 12 sequencing prep reactions 3 ligation 5 ligation reverse transcription cDNA cleanup library amplification and library cleanup reagents quality control primers Benefits Gel free miRNA sequencing library prep from as little as 1 ng of total RNA Elimination of adapter dimers and unwanted RNA species resulting in the highest fidelity and most efficient data Bead based method to remove adapter dimers and unwanted RNA species Integrated Unique Molecular Indices UMIs enable quantification of individual miRNA molecules Primary read mapping and differential expression analysis via the GeneGlobe Data Analysis Cen
    Catalog Number:
    331502
    Price:
    1120
    Category:
    QIAseq miRNA Library Kit
    Buy from Supplier


    Structured Review

    Qiagen qiaseq mirna library kit
    QIAseq miRNA Library Kit
    Gel free miRNA Sample to Insight solution for differential expression analysis and novel discovery using next generation sequencing Kit contents For 12 sequencing prep reactions 3 ligation 5 ligation reverse transcription cDNA cleanup library amplification and library cleanup reagents quality control primers Benefits Gel free miRNA sequencing library prep from as little as 1 ng of total RNA Elimination of adapter dimers and unwanted RNA species resulting in the highest fidelity and most efficient data Bead based method to remove adapter dimers and unwanted RNA species Integrated Unique Molecular Indices UMIs enable quantification of individual miRNA molecules Primary read mapping and differential expression analysis via the GeneGlobe Data Analysis Cen
    https://www.bioz.com/result/qiaseq mirna library kit/product/Qiagen
    Average 90 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    qiaseq mirna library kit - by Bioz Stars, 2020-01
    90/100 stars

    Images

    Related Articles

    Amplification:

    Article Title: Bulky DNA adducts, microRNA profiles, and lipid biomarkers in Norwegian tunnel finishing workers occupationally exposed to diesel exhaust
    Article Snippet: Small RNA sequencing (sRNA-seq) Libraries for sRNA-seq were prepared using QIAseq miRNA Library kit (Qiagen, Hilden, Germany). .. Briefly, adapters were ligated to the 3' and 5' ends of miRNAs. sRNAs with ligated adapters underwent reverse transcription with unique molecular identifier (UMI) assignment followed by library amplification (16 cycles) on a Mastercycler Nexus (Eppendorf, Hamburg, Germany). sRNA libraries were validated on an Agilent 2100 Bioanalyzer (Agilent, Santa Clara, California, USA) using high-sensitivity DNA chips.

    Article Title: Evaluation of methodologies for microRNA biomarker detection by next generation sequencing
    Article Snippet: As such, certain adapter-miRNA pairs or sequence compositions may be favoured over others during ligation and PCR amplification, and thereby result in over- or underrepresentation of these miRNAs in the sequencing library [ , ]. .. In this study, we have benchmarked four commercial kits intended for Illumina sequencing: Nextflex Small RNA-Seq Kit v3, Bioo Scientific (BSC); SMARTer smRNA-Seq Kit, Clontech/Takara (CLT); NEBNext Small RNA Library Prep Set, New England Biolabs (NEB) and QIAseq miRNA Library Kit, Qiagen (QIA).

    Article Title: Mesenchymal Stem Cells Reduce Corneal Fibrosis and Inflammation via Extracellular Vesicle‐Mediated Delivery of miRNA
    Article Snippet: As a control for amplification or losses during the assay process, a nonmammalian miRNA “spike‐in” (ath‐miR159a) was added to a known concentration to the RNA samples before reverse transcription. miRNA content of CSSC‐derived EVs was determined for 800 human miRNA species by screening RNA isolated from EVs produced by CSSC isolated from eight different donors, from human corneal fibroblasts, and from HEK293T cells using a Nanostring Array (Nanostring, Inc., CSO‐MIR3‐12). .. A strand‐specific small RNA library was produced from EV RNA using the QIAseq miRNA library kit (Qiagen) following the manufacturer's instructions.

    Article Title: Dose-dependent effects of chronic alcohol drinking on peripheral immune responses
    Article Snippet: Small RNA library preparation Small RNA libraries were generated from total RNA as starting material using QIAseq miRNA Library kit (Qiagen, Valencia CA) per manufacturer’s instructions. .. Following reverse transcription (adapters 1:5 dilution), cDNA was size selected using QIASeq miRNA NGS beads and PCR amplified for 19 cycles.

    Article Title: HNRNPA2/B1 is upregulated in endocrine-resistant LCC9 breast cancer cells and alters the miRNA transcriptome when overexpressed in MCF-7 cells
    Article Snippet: Libraries were prepared using QIAseq miRNA Library Kit (Qiagen). cDNA samples were barcoded with QIAseq miRNA NGS ILM IP primers. .. Adaptor dimers were removed from amplified libraries using QIAseq miRNA NGS beads.

    Construct:

    Article Title: Systematic Analysis of Transcriptomic Profile of the Effects of Low Dose Atropine Treatment on Scleral Fibroblasts using Next-Generation Sequencing and Bioinformatics
    Article Snippet: Samples were prepared with the QIAseq miRNA Library Kit (QIAGEN) following the instruction manual. .. Following the sequential ligation of adaptors to the 3' and 5' ends of miRNAs, universal cDNA synthesis was carried out, and the cDNA constructs containing 18-40 nucleotide (140-155 nucleotides in length with both adaptors) RNA fragments were chosen.

    Incubation:

    Article Title: eNOS expression and NO release during hypoxia is inhibited by miR-200b in human endothelial cells
    Article Snippet: Next generation sequencing analyses of miRNAs HUVECs at passage 3 were incubated under normoxic or hypoxic conditions for 16 h, and subsequently used for the RNA isolation and analyses. .. MiRNA sequencing libraries were prepared using QIAseq miRNA library kit (Qiagen) following the manufacturer’s instructions and followed by sequencing on an Illumina NextSeq instrument.

    Significance Assay:

    Article Title: Immunophenotyping and transcriptional profiling of in vitro cultured human adipose tissue derived stem cells
    Article Snippet: Transcripts that showed significant and consistent differences between conditions in our dataset (FDR corrected p-value, q-value < 0.05) were subjected to further interpretation with Ingenuity Pathway Analysis. .. 2 combination (Supplementary Table ). miRNA-seq was performed for the same set of five biological replicate experiments using QIAseq miRNA Library Kit (Qiagen, Hilden, Germany) following the manufacturer protocol, starting with 500 ng of input RNA for each sample.

    Expressing:

    Article Title: PIWI proteins contribute to apoptosis during the UPR in human airway epithelial cells
    Article Snippet: Small RNA sequencing libraries were prepared using QIAseq miRNA library kit (Qiagen) following the manufacturer’s instructions and followed by sequencing on an Illumina NextSeq instrument. .. The analysis of the differential expression of small RNAs between control and experimental samples were performed with geNorm in the Gene Globe Software.

    Article Title: eNOS expression and NO release during hypoxia is inhibited by miR-200b in human endothelial cells
    Article Snippet: MiRNA sequencing libraries were prepared using QIAseq miRNA library kit (Qiagen) following the manufacturer’s instructions and followed by sequencing on an Illumina NextSeq instrument. .. The analyses of the differential expression of small RNAs between the control (normoxia) and the experimental sample (16 h hypoxia) were performed with geNorm [ ] the Gene Globe Software.

    Article Title: Systematic Analysis of Transcriptomic Profile of the Effects of Low Dose Atropine Treatment on Scleral Fibroblasts using Next-Generation Sequencing and Bioinformatics
    Article Snippet: Paragraph title: Next-Generation Sequencing (NGS) for miRNA and mRNA Expression Profiling ... Samples were prepared with the QIAseq miRNA Library Kit (QIAGEN) following the instruction manual.

    Modification:

    Article Title: A comparison of RNA extraction and sequencing protocols for detection of small RNAs in plasma
    Article Snippet: CleanTag™ Small RNA Library Prep Kit (TriLink) uses modified adapters to reduce adapter dimer formation from low inputs of RNA [ ]. .. QIAseq miRNA Library Kit (QIAGEN) claims to employ optimised reaction chemistry to reduce bias, minimise adapter dimer formation and contaminating non-miRNAs, facilitating low inputs of RNA.

    Derivative Assay:

    Article Title: Evaluation of methodologies for microRNA biomarker detection by next generation sequencing
    Article Snippet: In this study, we have benchmarked four commercial kits intended for Illumina sequencing: Nextflex Small RNA-Seq Kit v3, Bioo Scientific (BSC); SMARTer smRNA-Seq Kit, Clontech/Takara (CLT); NEBNext Small RNA Library Prep Set, New England Biolabs (NEB) and QIAseq miRNA Library Kit, Qiagen (QIA). .. All methodologies can generate miRNA sequencing libraries from low input amounts (1–100 ng) and are therefore suitable to process RNA derived from biofluids.

    Ligation:

    Article Title: Evaluation of methodologies for microRNA biomarker detection by next generation sequencing
    Article Snippet: As such, certain adapter-miRNA pairs or sequence compositions may be favoured over others during ligation and PCR amplification, and thereby result in over- or underrepresentation of these miRNAs in the sequencing library [ , ]. .. In this study, we have benchmarked four commercial kits intended for Illumina sequencing: Nextflex Small RNA-Seq Kit v3, Bioo Scientific (BSC); SMARTer smRNA-Seq Kit, Clontech/Takara (CLT); NEBNext Small RNA Library Prep Set, New England Biolabs (NEB) and QIAseq miRNA Library Kit, Qiagen (QIA).

    Article Title: Systematic Analysis of Transcriptomic Profile of the Effects of Low Dose Atropine Treatment on Scleral Fibroblasts using Next-Generation Sequencing and Bioinformatics
    Article Snippet: Samples were prepared with the QIAseq miRNA Library Kit (QIAGEN) following the instruction manual. .. Following the sequential ligation of adaptors to the 3' and 5' ends of miRNAs, universal cDNA synthesis was carried out, and the cDNA constructs containing 18-40 nucleotide (140-155 nucleotides in length with both adaptors) RNA fragments were chosen.

    Biomarker Assay:

    Article Title: A comparison of RNA extraction and sequencing protocols for detection of small RNAs in plasma
    Article Snippet: Issues particularly pertinent for blood-based biomarker discovery are the ability to work with low miRNA concentrations and reduction of bias. .. QIAseq miRNA Library Kit (QIAGEN) claims to employ optimised reaction chemistry to reduce bias, minimise adapter dimer formation and contaminating non-miRNAs, facilitating low inputs of RNA.

    Indirect Immunoperoxidase Assay:

    Article Title: Immunophenotyping and transcriptional profiling of in vitro cultured human adipose tissue derived stem cells
    Article Snippet: Differentially expressed genes were grouped in functional pathways using Ingenuity Pathway Analysis (IPA Spring Release 2017, Redwood City, USA) on gene level using RefSeq annotation. .. 2 combination (Supplementary Table ). miRNA-seq was performed for the same set of five biological replicate experiments using QIAseq miRNA Library Kit (Qiagen, Hilden, Germany) following the manufacturer protocol, starting with 500 ng of input RNA for each sample.

    Generated:

    Article Title: Dose-dependent effects of chronic alcohol drinking on peripheral immune responses
    Article Snippet: .. Small RNA library preparation Small RNA libraries were generated from total RNA as starting material using QIAseq miRNA Library kit (Qiagen, Valencia CA) per manufacturer’s instructions. ..

    Sequencing:

    Article Title: A comparison of RNA extraction and sequencing protocols for detection of small RNAs in plasma
    Article Snippet: NEXTflex® Small RNA Sequencing Kit v3 (Bioo Scientific) uses randomised adapters to reduce sequencing bias and adapter dimer reduction technology to allow low inputs of RNA [ ]. .. QIAseq miRNA Library Kit (QIAGEN) claims to employ optimised reaction chemistry to reduce bias, minimise adapter dimer formation and contaminating non-miRNAs, facilitating low inputs of RNA.

    Article Title: PIWI proteins contribute to apoptosis during the UPR in human airway epithelial cells
    Article Snippet: .. Small RNA sequencing libraries were prepared using QIAseq miRNA library kit (Qiagen) following the manufacturer’s instructions and followed by sequencing on an Illumina NextSeq instrument. .. Using Qiagen’s Gene Globe Software, sequencing reads were aligned to the human reference genome assembly (hg19) followed by transcript assembly and estimation of the relative abundances.

    Article Title: Immunophenotyping and transcriptional profiling of in vitro cultured human adipose tissue derived stem cells
    Article Snippet: Paragraph title: RNA- and miRNA Sequencing and Bioinformatic Analysis ... 2 combination (Supplementary Table ). miRNA-seq was performed for the same set of five biological replicate experiments using QIAseq miRNA Library Kit (Qiagen, Hilden, Germany) following the manufacturer protocol, starting with 500 ng of input RNA for each sample.

    Article Title: Bulky DNA adducts, microRNA profiles, and lipid biomarkers in Norwegian tunnel finishing workers occupationally exposed to diesel exhaust
    Article Snippet: Small RNA sequencing (sRNA-seq) Libraries for sRNA-seq were prepared using QIAseq miRNA Library kit (Qiagen, Hilden, Germany). .. Sequencing of sRNA libraries was performed on MiSeq system (Illumina, San Diego, California, USA) using MiSeq Reagent Kit V.3.

    Article Title: Evaluation of methodologies for microRNA biomarker detection by next generation sequencing
    Article Snippet: .. In this study, we have benchmarked four commercial kits intended for Illumina sequencing: Nextflex Small RNA-Seq Kit v3, Bioo Scientific (BSC); SMARTer smRNA-Seq Kit, Clontech/Takara (CLT); NEBNext Small RNA Library Prep Set, New England Biolabs (NEB) and QIAseq miRNA Library Kit, Qiagen (QIA). .. All methodologies can generate miRNA sequencing libraries from low input amounts (1–100 ng) and are therefore suitable to process RNA derived from biofluids.

    Article Title: Mesenchymal Stem Cells Reduce Corneal Fibrosis and Inflammation via Extracellular Vesicle‐Mediated Delivery of miRNA
    Article Snippet: RNA sequence analysis. .. A strand‐specific small RNA library was produced from EV RNA using the QIAseq miRNA library kit (Qiagen) following the manufacturer's instructions.

    Article Title: Exosomes from Endothelial Progenitor Cells Improve the Outcome of a Murine Model of Sepsis
    Article Snippet: Extracted RNA (100 ng) was used to prepare miRNA-focused NGS libraries using QIAseq miRNA Library Kit (QIAGEN, Germantown, MD, USA). .. The sequencing was performed on an Illumina HiSeq 2500 instrument at the MUSC Genomic Sequencing Core Facility.

    Article Title: A comparison of RNA extraction and sequencing protocols for detection of small RNAs in plasma
    Article Snippet: .. Library preparation and sequencing Libraries were prepared from 5 μl of miRNeasy RNA using NEXTflex® Small RNA Sequencing Kit v3 for Illumina® Platforms (Bioo Scientific) and QIAseq miRNA Library Kit (QIAGEN) and from 2 μl miRNeasy RNA using CleanTag™ Small RNA Library Prep Kit (TriLink), following each of the manufacturers’ instructions. .. Additionally, libraries were prepared from 5 μl MagnaZol RNA using NEXTflex® Small RNA Sequencing Kit v3 for Illumina® Platforms (Bioo Scientific) and QIAseq miRNA Library Kit (QIAGEN).

    Article Title: eNOS expression and NO release during hypoxia is inhibited by miR-200b in human endothelial cells
    Article Snippet: .. MiRNA sequencing libraries were prepared using QIAseq miRNA library kit (Qiagen) following the manufacturer’s instructions and followed by sequencing on an Illumina NextSeq instrument. .. Using Qiagen’s Gene Globe Software, sequencing reads were aligned to the human reference genome assembly (hg19) followed by transcript assembly and estimation of the relative abundances.

    Article Title: Systematic Analysis of Transcriptomic Profile of the Effects of Low Dose Atropine Treatment on Scleral Fibroblasts using Next-Generation Sequencing and Bioinformatics
    Article Snippet: Samples were prepared with the QIAseq miRNA Library Kit (QIAGEN) following the instruction manual. .. Sequencing data was processed by Illumina software.

    RNA Sequencing Assay:

    Article Title: A comparison of RNA extraction and sequencing protocols for detection of small RNAs in plasma
    Article Snippet: NEXTflex® Small RNA Sequencing Kit v3 (Bioo Scientific) uses randomised adapters to reduce sequencing bias and adapter dimer reduction technology to allow low inputs of RNA [ ]. .. QIAseq miRNA Library Kit (QIAGEN) claims to employ optimised reaction chemistry to reduce bias, minimise adapter dimer formation and contaminating non-miRNAs, facilitating low inputs of RNA.

    Article Title: PIWI proteins contribute to apoptosis during the UPR in human airway epithelial cells
    Article Snippet: .. Small RNA sequencing libraries were prepared using QIAseq miRNA library kit (Qiagen) following the manufacturer’s instructions and followed by sequencing on an Illumina NextSeq instrument. .. Using Qiagen’s Gene Globe Software, sequencing reads were aligned to the human reference genome assembly (hg19) followed by transcript assembly and estimation of the relative abundances.

    Article Title: Inheritance of a Phenotypically Neutral Epimutation Evokes Gene Silencing in Later Generations
    Article Snippet: Paragraph title: Small RNA sequencing and analysis ... Small RNA libraries were prepared with the QIAseq miRNA Library Kit (QIAGEN) according to manufacturer’s instructions and sequenced with an Illumina HiSeq2500.

    Article Title: Bulky DNA adducts, microRNA profiles, and lipid biomarkers in Norwegian tunnel finishing workers occupationally exposed to diesel exhaust
    Article Snippet: .. Small RNA sequencing (sRNA-seq) Libraries for sRNA-seq were prepared using QIAseq miRNA Library kit (Qiagen, Hilden, Germany). .. Briefly, adapters were ligated to the 3' and 5' ends of miRNAs. sRNAs with ligated adapters underwent reverse transcription with unique molecular identifier (UMI) assignment followed by library amplification (16 cycles) on a Mastercycler Nexus (Eppendorf, Hamburg, Germany). sRNA libraries were validated on an Agilent 2100 Bioanalyzer (Agilent, Santa Clara, California, USA) using high-sensitivity DNA chips.

    Article Title: Evaluation of methodologies for microRNA biomarker detection by next generation sequencing
    Article Snippet: .. In this study, we have benchmarked four commercial kits intended for Illumina sequencing: Nextflex Small RNA-Seq Kit v3, Bioo Scientific (BSC); SMARTer smRNA-Seq Kit, Clontech/Takara (CLT); NEBNext Small RNA Library Prep Set, New England Biolabs (NEB) and QIAseq miRNA Library Kit, Qiagen (QIA). .. All methodologies can generate miRNA sequencing libraries from low input amounts (1–100 ng) and are therefore suitable to process RNA derived from biofluids.

    Article Title: A comparison of RNA extraction and sequencing protocols for detection of small RNAs in plasma
    Article Snippet: .. Library preparation and sequencing Libraries were prepared from 5 μl of miRNeasy RNA using NEXTflex® Small RNA Sequencing Kit v3 for Illumina® Platforms (Bioo Scientific) and QIAseq miRNA Library Kit (QIAGEN) and from 2 μl miRNeasy RNA using CleanTag™ Small RNA Library Prep Kit (TriLink), following each of the manufacturers’ instructions. .. Additionally, libraries were prepared from 5 μl MagnaZol RNA using NEXTflex® Small RNA Sequencing Kit v3 for Illumina® Platforms (Bioo Scientific) and QIAseq miRNA Library Kit (QIAGEN).

    Isolation:

    Article Title: A comparison of RNA extraction and sequencing protocols for detection of small RNAs in plasma
    Article Snippet: QIAseq miRNA Library Kit (QIAGEN) claims to employ optimised reaction chemistry to reduce bias, minimise adapter dimer formation and contaminating non-miRNAs, facilitating low inputs of RNA. .. The MagnaZol™ cfRNA Isolation Reagent (Bioo Scientific) and miRNeasy Serum/Plasma kit (QIAGEN) are RNA extraction kits for the extraction of small RNAs from biofluids, specifically designed to work with low input volumes of plasma.

    Article Title: PIWI proteins contribute to apoptosis during the UPR in human airway epithelial cells
    Article Snippet: Next generation small RNAs sequencing analyses 16HBE14o- cells were used for the RNA isolation and analyses. .. Small RNA sequencing libraries were prepared using QIAseq miRNA library kit (Qiagen) following the manufacturer’s instructions and followed by sequencing on an Illumina NextSeq instrument.

    Article Title: Mesenchymal Stem Cells Reduce Corneal Fibrosis and Inflammation via Extracellular Vesicle‐Mediated Delivery of miRNA
    Article Snippet: Paragraph title: miRNA Isolation and Analysis ... A strand‐specific small RNA library was produced from EV RNA using the QIAseq miRNA library kit (Qiagen) following the manufacturer's instructions.

    Article Title: HNRNPA2/B1 is upregulated in endocrine-resistant LCC9 breast cancer cells and alters the miRNA transcriptome when overexpressed in MCF-7 cells
    Article Snippet: For miRNA-seq miRNA was isolated from six separate, biological replicate experiments for each sample group (Control, HNRNPA2/B1 48 h, HNRNPA2/B172 h) using the miRNeasy mini kit from Qiagen (Hilden, Germany) following the manufacturer’s directions. .. Libraries were prepared using QIAseq miRNA Library Kit (Qiagen). cDNA samples were barcoded with QIAseq miRNA NGS ILM IP primers.

    Article Title: eNOS expression and NO release during hypoxia is inhibited by miR-200b in human endothelial cells
    Article Snippet: Next generation sequencing analyses of miRNAs HUVECs at passage 3 were incubated under normoxic or hypoxic conditions for 16 h, and subsequently used for the RNA isolation and analyses. .. MiRNA sequencing libraries were prepared using QIAseq miRNA library kit (Qiagen) following the manufacturer’s instructions and followed by sequencing on an Illumina NextSeq instrument.

    RNA Extraction:

    Article Title: A comparison of RNA extraction and sequencing protocols for detection of small RNAs in plasma
    Article Snippet: QIAseq miRNA Library Kit (QIAGEN) claims to employ optimised reaction chemistry to reduce bias, minimise adapter dimer formation and contaminating non-miRNAs, facilitating low inputs of RNA. .. RNA extraction methods have been reported to affect the profile of miRNAs detected [ , , , ].

    Purification:

    Article Title: Inheritance of a Phenotypically Neutral Epimutation Evokes Gene Silencing in Later Generations
    Article Snippet: Total RNA from exponentially growing cultures was extracted with the MasterPure Yeast RNA Purification Kit (Epicenter). .. Small RNA libraries were prepared with the QIAseq miRNA Library Kit (QIAGEN) according to manufacturer’s instructions and sequenced with an Illumina HiSeq2500.

    Article Title: Systematic Analysis of Transcriptomic Profile of the Effects of Low Dose Atropine Treatment on Scleral Fibroblasts using Next-Generation Sequencing and Bioinformatics
    Article Snippet: Purified RNA was quantified at an optical density of 260nm with a ND-1000 spectrophotometer (Nanodrop Technologies, Wilmington, DE, USA). .. Samples were prepared with the QIAseq miRNA Library Kit (QIAGEN) following the instruction manual.

    Polymerase Chain Reaction:

    Article Title: A comparison of RNA extraction and sequencing protocols for detection of small RNAs in plasma
    Article Snippet: QIAseq miRNA Library Kit (QIAGEN) claims to employ optimised reaction chemistry to reduce bias, minimise adapter dimer formation and contaminating non-miRNAs, facilitating low inputs of RNA. .. QIAseq is the only kit to incorporate unique molecular indices (UMIs) into each cDNA to enable correction for PCR bias.

    Article Title: Evaluation of methodologies for microRNA biomarker detection by next generation sequencing
    Article Snippet: As such, certain adapter-miRNA pairs or sequence compositions may be favoured over others during ligation and PCR amplification, and thereby result in over- or underrepresentation of these miRNAs in the sequencing library [ , ]. .. In this study, we have benchmarked four commercial kits intended for Illumina sequencing: Nextflex Small RNA-Seq Kit v3, Bioo Scientific (BSC); SMARTer smRNA-Seq Kit, Clontech/Takara (CLT); NEBNext Small RNA Library Prep Set, New England Biolabs (NEB) and QIAseq miRNA Library Kit, Qiagen (QIA).

    Article Title: Dose-dependent effects of chronic alcohol drinking on peripheral immune responses
    Article Snippet: Small RNA library preparation Small RNA libraries were generated from total RNA as starting material using QIAseq miRNA Library kit (Qiagen, Valencia CA) per manufacturer’s instructions. .. Following reverse transcription (adapters 1:5 dilution), cDNA was size selected using QIASeq miRNA NGS beads and PCR amplified for 19 cycles.

    Software:

    Article Title: PIWI proteins contribute to apoptosis during the UPR in human airway epithelial cells
    Article Snippet: Small RNA sequencing libraries were prepared using QIAseq miRNA library kit (Qiagen) following the manufacturer’s instructions and followed by sequencing on an Illumina NextSeq instrument. .. Using Qiagen’s Gene Globe Software, sequencing reads were aligned to the human reference genome assembly (hg19) followed by transcript assembly and estimation of the relative abundances.

    Article Title: Mesenchymal Stem Cells Reduce Corneal Fibrosis and Inflammation via Extracellular Vesicle‐Mediated Delivery of miRNA
    Article Snippet: Relative abundance was determined using nSolver software. .. A strand‐specific small RNA library was produced from EV RNA using the QIAseq miRNA library kit (Qiagen) following the manufacturer's instructions.

    Article Title: eNOS expression and NO release during hypoxia is inhibited by miR-200b in human endothelial cells
    Article Snippet: MiRNA sequencing libraries were prepared using QIAseq miRNA library kit (Qiagen) following the manufacturer’s instructions and followed by sequencing on an Illumina NextSeq instrument. .. Using Qiagen’s Gene Globe Software, sequencing reads were aligned to the human reference genome assembly (hg19) followed by transcript assembly and estimation of the relative abundances.

    Article Title: Systematic Analysis of Transcriptomic Profile of the Effects of Low Dose Atropine Treatment on Scleral Fibroblasts using Next-Generation Sequencing and Bioinformatics
    Article Snippet: Samples were prepared with the QIAseq miRNA Library Kit (QIAGEN) following the instruction manual. .. Sequencing data was processed by Illumina software.

    Functional Assay:

    Article Title: Immunophenotyping and transcriptional profiling of in vitro cultured human adipose tissue derived stem cells
    Article Snippet: Differentially expressed genes were grouped in functional pathways using Ingenuity Pathway Analysis (IPA Spring Release 2017, Redwood City, USA) on gene level using RefSeq annotation. .. 2 combination (Supplementary Table ). miRNA-seq was performed for the same set of five biological replicate experiments using QIAseq miRNA Library Kit (Qiagen, Hilden, Germany) following the manufacturer protocol, starting with 500 ng of input RNA for each sample.

    Selection:

    Article Title: Evaluation of methodologies for microRNA biomarker detection by next generation sequencing
    Article Snippet: In order to generate small RNA sequencing (sRNA-seq) libraries, adapters are ligated to both ends of the miRNA, followed by reverse transcription, template amplification by PCR and size selection of small RNA species. .. In this study, we have benchmarked four commercial kits intended for Illumina sequencing: Nextflex Small RNA-Seq Kit v3, Bioo Scientific (BSC); SMARTer smRNA-Seq Kit, Clontech/Takara (CLT); NEBNext Small RNA Library Prep Set, New England Biolabs (NEB) and QIAseq miRNA Library Kit, Qiagen (QIA).

    Next-Generation Sequencing:

    Article Title: Dose-dependent effects of chronic alcohol drinking on peripheral immune responses
    Article Snippet: Small RNA library preparation Small RNA libraries were generated from total RNA as starting material using QIAseq miRNA Library kit (Qiagen, Valencia CA) per manufacturer’s instructions. .. Following reverse transcription (adapters 1:5 dilution), cDNA was size selected using QIASeq miRNA NGS beads and PCR amplified for 19 cycles.

    Article Title: HNRNPA2/B1 is upregulated in endocrine-resistant LCC9 breast cancer cells and alters the miRNA transcriptome when overexpressed in MCF-7 cells
    Article Snippet: .. Libraries were prepared using QIAseq miRNA Library Kit (Qiagen). cDNA samples were barcoded with QIAseq miRNA NGS ILM IP primers. .. Adaptor dimers were removed from amplified libraries using QIAseq miRNA NGS beads.

    Article Title: Exosomes from Endothelial Progenitor Cells Improve the Outcome of a Murine Model of Sepsis
    Article Snippet: .. Extracted RNA (100 ng) was used to prepare miRNA-focused NGS libraries using QIAseq miRNA Library Kit (QIAGEN, Germantown, MD, USA). .. The sequencing was performed on an Illumina HiSeq 2500 instrument at the MUSC Genomic Sequencing Core Facility.

    Article Title: eNOS expression and NO release during hypoxia is inhibited by miR-200b in human endothelial cells
    Article Snippet: Paragraph title: Next generation sequencing analyses of miRNAs ... MiRNA sequencing libraries were prepared using QIAseq miRNA library kit (Qiagen) following the manufacturer’s instructions and followed by sequencing on an Illumina NextSeq instrument.

    Article Title: Systematic Analysis of Transcriptomic Profile of the Effects of Low Dose Atropine Treatment on Scleral Fibroblasts using Next-Generation Sequencing and Bioinformatics
    Article Snippet: Paragraph title: Next-Generation Sequencing (NGS) for miRNA and mRNA Expression Profiling ... Samples were prepared with the QIAseq miRNA Library Kit (QIAGEN) following the instruction manual.

    Spectrophotometry:

    Article Title: Systematic Analysis of Transcriptomic Profile of the Effects of Low Dose Atropine Treatment on Scleral Fibroblasts using Next-Generation Sequencing and Bioinformatics
    Article Snippet: Purified RNA was quantified at an optical density of 260nm with a ND-1000 spectrophotometer (Nanodrop Technologies, Wilmington, DE, USA). .. Samples were prepared with the QIAseq miRNA Library Kit (QIAGEN) following the instruction manual.

    Produced:

    Article Title: Mesenchymal Stem Cells Reduce Corneal Fibrosis and Inflammation via Extracellular Vesicle‐Mediated Delivery of miRNA
    Article Snippet: .. A strand‐specific small RNA library was produced from EV RNA using the QIAseq miRNA library kit (Qiagen) following the manufacturer's instructions. .. Postlibrary concentrations were quantified using Qubit Fluorometric Quantification apparatus (Thermo Fisher Scientific, MA).

    Concentration Assay:

    Article Title: Mesenchymal Stem Cells Reduce Corneal Fibrosis and Inflammation via Extracellular Vesicle‐Mediated Delivery of miRNA
    Article Snippet: As a control for amplification or losses during the assay process, a nonmammalian miRNA “spike‐in” (ath‐miR159a) was added to a known concentration to the RNA samples before reverse transcription. miRNA content of CSSC‐derived EVs was determined for 800 human miRNA species by screening RNA isolated from EVs produced by CSSC isolated from eight different donors, from human corneal fibroblasts, and from HEK293T cells using a Nanostring Array (Nanostring, Inc., CSO‐MIR3‐12). .. A strand‐specific small RNA library was produced from EV RNA using the QIAseq miRNA library kit (Qiagen) following the manufacturer's instructions.

    Article Title: A comparison of RNA extraction and sequencing protocols for detection of small RNAs in plasma
    Article Snippet: Library preparation and sequencing Libraries were prepared from 5 μl of miRNeasy RNA using NEXTflex® Small RNA Sequencing Kit v3 for Illumina® Platforms (Bioo Scientific) and QIAseq miRNA Library Kit (QIAGEN) and from 2 μl miRNeasy RNA using CleanTag™ Small RNA Library Prep Kit (TriLink), following each of the manufacturers’ instructions. .. Quality and concentration of libraries were determined by Fragment Analyzer (Advanced Analytical).

    Genomic Sequencing:

    Article Title: Exosomes from Endothelial Progenitor Cells Improve the Outcome of a Murine Model of Sepsis
    Article Snippet: Extracted RNA (100 ng) was used to prepare miRNA-focused NGS libraries using QIAseq miRNA Library Kit (QIAGEN, Germantown, MD, USA). .. The sequencing was performed on an Illumina HiSeq 2500 instrument at the MUSC Genomic Sequencing Core Facility.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90
    Qiagen qiaseq mirna library kit
    Qiaseq Mirna Library Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qiaseq mirna library kit/product/Qiagen
    Average 90 stars, based on 17 article reviews
    Price from $9.99 to $1999.99
    qiaseq mirna library kit - by Bioz Stars, 2020-01
    90/100 stars
      Buy from Supplier

    N/A
    Gel free miRNA Sample to Insight solution for differential expression analysis and novel discovery using next generation sequencing Kit contents Sequencing adapters primers and indexes compatible with Illumina platforms 12
      Buy from Supplier

    N/A
    For recreating Screening Suite conditions Kit contents 1M BisTris pH 7 5 100 ml
      Buy from Supplier

    Image Search Results