rt² sybr green rox qpcr mastermix  (Qiagen)


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    RT² SYBR Green ROX qPCR Mastermix
    Description:
    For use with RT2 Profiler RT2 lncRNA EpiTect ChIP and EpiTect Methyl II PCR Arrays and qPCR Assays Kit contents Contains 2 x 1 35 ml tubes for 2 x 96 well RT PCR Arrays 1 x 384 well RT PCR Array or 200 x 25 l reactionsor 400 x 10l reactions Benefits Designed exclusively for use with RT 2 and EpiTect Methyl II qPCR Assays Delivers specific amplification with minimal primer dimers Available with ROX fluorescein or without dye For use with qPCR instruments under standard cycling conditions nbsp
    Catalog Number:
    330520
    Price:
    199
    Category:
    RT2 SYBR Green qPCR Mastermixes
    Buy from Supplier


    Structured Review

    Qiagen rt² sybr green rox qpcr mastermix
    RT² SYBR Green ROX qPCR Mastermix
    For use with RT2 Profiler RT2 lncRNA EpiTect ChIP and EpiTect Methyl II PCR Arrays and qPCR Assays Kit contents Contains 2 x 1 35 ml tubes for 2 x 96 well RT PCR Arrays 1 x 384 well RT PCR Array or 200 x 25 l reactionsor 400 x 10l reactions Benefits Designed exclusively for use with RT 2 and EpiTect Methyl II qPCR Assays Delivers specific amplification with minimal primer dimers Available with ROX fluorescein or without dye For use with qPCR instruments under standard cycling conditions nbsp
    https://www.bioz.com/result/rt² sybr green rox qpcr mastermix/product/Qiagen
    Average 90 stars, based on 63 article reviews
    Price from $9.99 to $1999.99
    rt² sybr green rox qpcr mastermix - by Bioz Stars, 2020-01
    90/100 stars

    Images

    1) Product Images from "Innate And Adaptive Immunity are Progressively Activated in Parallel with Renal Injury in the 5/6 Renal Ablation Model"

    Article Title: Innate And Adaptive Immunity are Progressively Activated in Parallel with Renal Injury in the 5/6 Renal Ablation Model

    Journal: Scientific Reports

    doi: 10.1038/s41598-017-02915-6

    Quantitative PCR analysis (qPCR) for Tlr4 ( A ), Nlrp3 ( B ), Casp1 ( C ) and Il1b ( D ) gene expression. Results are presented as median plus range. The number of analyzed animals is represented next to the name of each group. Differences among groups were analyzed by one-way ANOVA with Newman-Keuls post test. a p
    Figure Legend Snippet: Quantitative PCR analysis (qPCR) for Tlr4 ( A ), Nlrp3 ( B ), Casp1 ( C ) and Il1b ( D ) gene expression. Results are presented as median plus range. The number of analyzed animals is represented next to the name of each group. Differences among groups were analyzed by one-way ANOVA with Newman-Keuls post test. a p

    Techniques Used: Real-time Polymerase Chain Reaction, Expressing

    2) Product Images from "Normal stroma suppresses cancer cell proliferation via mechanosensitive regulation of JMJD1a-mediated transcription"

    Article Title: Normal stroma suppresses cancer cell proliferation via mechanosensitive regulation of JMJD1a-mediated transcription

    Journal: Nature Communications

    doi: 10.1038/ncomms12237

    JMJD1a regulates YAP/TAZ transcription ( a ) Representative western blot showing YAP/TAZ expression in MDA-MB-231 on CDM and on plastic. ( b ) Taqman qRT–PCR of CTGF ( n =4) and THBS1 ( n =5) mRNA levels in MDA-MB-231 cells grown on CDM or on plastic. ( c ) Quantification of JMJD1a and YAP/TAZ staining intensity from immunofluorescence images of MDA-MB-231 cells on plastic. Intensity (int den) was quantified using the CellProfiler software. n (cells)=146. R -value indicates correlation. ( d ) ChIP showing the binding of JMJD1a to the TAZ promoter. Analysis was performed by SYBR green-based detection and fold increase in signal relative to the background signal (IgG control antibody) is shown. n =3 (mean±s.d.). Paired t -test was used to calculate P value. ( e ) ChIP showing H3K9me2 levels on TAZ promoter of siControl and siJMJD1a_3-transfected MDA-MB-231 cells. ChIP was performed with two independent H3K9me2 antibodies. Analysis was performed by SYBR green-based detection and fold increase in signal relative to the background signal (IgG control antibody) is shown. Representative results from two independent experiments. ( f , g ) A representative western blot ( e ) and quantification ( f ) showing YAP/TAZ expression in JMJD1a-silenced MDA-MB-231 cells after 3 days of silencing, n =7. ( h ) Taqman qRT–PCR of JMJD1a ( KDM3A ), CTGF and THBS1 mRNA levels in JMJD1a-silenced MDA-MB-231 cells, n =4. ( i ) Taqman qRT–PCR of YAP and TAZ mRNA levels in JMJD1a siRNA-transfected MDA-MB-231 cells. n (JMJD1a and TAZ)=4, n (YAP)=3. ( j , k ) Representative western blot ( i ) and quantification ( j ) showing YAP/TAZ expression in JMJD1a-overexpressing MDA-MB-231 cells normalized to loading control, n =4. ( l ) Taqman qRT–PCR of JMJD1a ( KDM3A) , CTGF and THBS1 mRNA levels in JMJD1a-overexpressing MDA-MB-231 cells, n =4. ( m ) A representative western blot and quantification of YAP/TAZ expression on TIFF-derived CDM in GFP control and JMJD1a-GFP-overexpressing cells after 4 days on CDM. ( n ) Representative western blot of JMJD1a and YAP/TAZ expression in GFP control and JMJD1a-overexpressing MDA-MB-231 cells plated on 4 or 50 kPa hydrogels or on PL.
    Figure Legend Snippet: JMJD1a regulates YAP/TAZ transcription ( a ) Representative western blot showing YAP/TAZ expression in MDA-MB-231 on CDM and on plastic. ( b ) Taqman qRT–PCR of CTGF ( n =4) and THBS1 ( n =5) mRNA levels in MDA-MB-231 cells grown on CDM or on plastic. ( c ) Quantification of JMJD1a and YAP/TAZ staining intensity from immunofluorescence images of MDA-MB-231 cells on plastic. Intensity (int den) was quantified using the CellProfiler software. n (cells)=146. R -value indicates correlation. ( d ) ChIP showing the binding of JMJD1a to the TAZ promoter. Analysis was performed by SYBR green-based detection and fold increase in signal relative to the background signal (IgG control antibody) is shown. n =3 (mean±s.d.). Paired t -test was used to calculate P value. ( e ) ChIP showing H3K9me2 levels on TAZ promoter of siControl and siJMJD1a_3-transfected MDA-MB-231 cells. ChIP was performed with two independent H3K9me2 antibodies. Analysis was performed by SYBR green-based detection and fold increase in signal relative to the background signal (IgG control antibody) is shown. Representative results from two independent experiments. ( f , g ) A representative western blot ( e ) and quantification ( f ) showing YAP/TAZ expression in JMJD1a-silenced MDA-MB-231 cells after 3 days of silencing, n =7. ( h ) Taqman qRT–PCR of JMJD1a ( KDM3A ), CTGF and THBS1 mRNA levels in JMJD1a-silenced MDA-MB-231 cells, n =4. ( i ) Taqman qRT–PCR of YAP and TAZ mRNA levels in JMJD1a siRNA-transfected MDA-MB-231 cells. n (JMJD1a and TAZ)=4, n (YAP)=3. ( j , k ) Representative western blot ( i ) and quantification ( j ) showing YAP/TAZ expression in JMJD1a-overexpressing MDA-MB-231 cells normalized to loading control, n =4. ( l ) Taqman qRT–PCR of JMJD1a ( KDM3A) , CTGF and THBS1 mRNA levels in JMJD1a-overexpressing MDA-MB-231 cells, n =4. ( m ) A representative western blot and quantification of YAP/TAZ expression on TIFF-derived CDM in GFP control and JMJD1a-GFP-overexpressing cells after 4 days on CDM. ( n ) Representative western blot of JMJD1a and YAP/TAZ expression in GFP control and JMJD1a-overexpressing MDA-MB-231 cells plated on 4 or 50 kPa hydrogels or on PL.

    Techniques Used: Western Blot, Expressing, Multiple Displacement Amplification, Quantitative RT-PCR, Staining, Immunofluorescence, Software, Chromatin Immunoprecipitation, Binding Assay, SYBR Green Assay, Transfection, Derivative Assay

    Related Articles

    Transduction:

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    Centrifugation:

    Article Title: Innate And Adaptive Immunity are Progressively Activated in Parallel with Renal Injury in the 5/6 Renal Ablation Model
    Article Snippet: Samples were stirred in the Omni Bead Ruptor 24® homogenizer, then subjected to a multi-step protocol of washing and centrifugation. .. The cDNAs were mixed with the appropriate PCR master mix buffer (Rt² Syber Green ROX qPCR Primer Assay QiagenN® #330523) and analyzed on specific array plates (Rat Innate & Adaptive Immune Responses Rt² Profiler™ PCR, Qiagen®, #330231-PARN052Zc).

    Amplification:

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    Article Snippet: Genomic DNA was amplified by real-time PCR with the 7500 Fast Real-Time PCR System (Applied Biosystems). .. Enzymes were inactivated, and the digested samples were used to seed quantitative PCR (qPCR) reactions using SYBR Green MasterMix (RT2 SYBR Green/ROX qPCR MasterMix, cat # 330520; Qiagen) and an Oxtr profiler primer.

    Article Title: Determinants of drug entry into the developing brain
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    Stable Transfection:

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    Synthesized:

    Article Title: Innate And Adaptive Immunity are Progressively Activated in Parallel with Renal Injury in the 5/6 Renal Ablation Model
    Article Snippet: After evaluation of RNA purity and final concentration, cDNA was synthesized through reverse transcription (RT) using the Rt² Easy First Strand kit (Qiagen® #330401). .. The cDNAs were mixed with the appropriate PCR master mix buffer (Rt² Syber Green ROX qPCR Primer Assay QiagenN® #330523) and analyzed on specific array plates (Rat Innate & Adaptive Immune Responses Rt² Profiler™ PCR, Qiagen®, #330231-PARN052Zc).

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    Article Title: Responses of Multipotent Retinal Stem Cells to IL-1β, IL-18, or IL-17
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    Quantitative RT-PCR:

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    Article Title: Determinants of drug entry into the developing brain
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    Article Title: Responses of Multipotent Retinal Stem Cells to IL-1β, IL-18, or IL-17
    Article Snippet: .. Quantitative RT-PCR (qRT-PCR) was performed using RT² SYBR Green ROX qPCR Mastermix (Qiagen). cDNA was amplified with primers β-actin , Il-6 , Il-17rc , or Il-17a (Qiagen) separately for 50 cycles. ..

    SYBR Green Assay:

    Article Title: The Tumor Suppressor Prostate Apoptosis Response-4 (Par-4) is Regulated by Mutant IDH1 and Kills Glioma Stem Cells
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    Article Title: Immune characteristics correlating with HSV‐1 immune control and effect of squaric acid dibutyl ester on immune characteristics of subjects with frequent herpes labialis episodes. Immune characteristics correlating with hsv‐1 immune control and effect of squaric acid dibutyl ester on immune characteristics of subjects with frequent herpes labialis episodes
    Article Snippet: .. 2.6.2 qPCR qPCR was done with the Qiagen RT PCR profiler gene analysis kit (catalog number 330451) using RT2 Sybr Green Rox qPCR Mastermix (24) (Part 330523) and Custom RT2 PCR array plates (Ref. 330171). .. For this 675 μL 2× RT2 SYBR green mastermix, 51 μL pre‐AMP synthesis reaction, and 623 μL RNase free water were mixed, and then 25 μL of the mixture was added to 48 wells on the left or right half of the custom PCR plate.

    Article Title: Transcriptional response to Wnt activation regulates the regenerative capacity of the mammalian cochlea
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    Two Tailed Test:

    Article Title: Transcriptional response to Wnt activation regulates the regenerative capacity of the mammalian cochlea
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    Luciferase:

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    Proliferation Assay:

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    Expressing:

    Article Title: The Tumor Suppressor Prostate Apoptosis Response-4 (Par-4) is Regulated by Mutant IDH1 and Kills Glioma Stem Cells
    Article Snippet: Briefly, U87 cells were harvested after treatment with or without 2-HG; cells stably expressing either vector-GFP or R132H IDH1-GFP were also harvested. .. The genomic DNAs were digested using the EpiTect Methyl II DNA Restriction Kit (Qiagen, Valencia, CA, Cat. No.335452) and real-time PCR was performed using RT SYBR Green ROX qPCR Mastermix (Qiagen, Valencia, CA, Cat. No 330520).

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    Article Title: Innate And Adaptive Immunity are Progressively Activated in Parallel with Renal Injury in the 5/6 Renal Ablation Model
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    Article Snippet: .. To evaluate expression of specific genes, RT-PCR reactions were performed on cDNA generated from total RNA isolated from the individual CSLC cultures using RT² qPCR Primer Assays and GAPDH (glyceraldehyde 3-phosphate dehydrogenase) as a constitutively active gene control, and RT² SYBR® Green/ROX qPCR Mastermix (Qiagen). .. Expression of selected genes was also determined in normal human lung and isolated normal human bronchial epithelial cells.

    Article Title: SP-A2 contributes to miRNA-mediated sex differences in response to oxidative stress: pro-inflammatory, anti-apoptotic, and anti-oxidant pathways are involved
    Article Snippet: Paragraph title: Gene expression assays ... Real-time PCR was performed using RT2 SYBR Green ROX qPCR Mastermix (#330520, Qiagen) on a QuantStudio 12K Flex Real-Time PCR System (Applied Biosystems, Waltham, MA) at the Pennsylvania State University College of Medicine Genomic Core Facility.

    Article Title: Determinants of drug entry into the developing brain
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    Article Title: Activation of Melatonin Signaling Promotes β-Cell Survival and Function
    Article Snippet: RNA abundance was quantified using the NanoDrop 1000 spectrophotometer (NanoDrop Technologies), and cDNA was synthesized from 0.5 μg total RNA using the RT2 first-strand kit (330520; QIAGEN) according to the manufacturer's instructions. .. Free web-based RT2 Profiler PCR Array Data Analysis Software (version 3.5; SABiosciences) was used to determine δδcycle threshold based fold-changes in expression of target genes using standardized settings (ie, baseline, threshold) and β-actin as the endogenous control gene across all plates.

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    Article Title: Transcriptional response to Wnt activation regulates the regenerative capacity of the mammalian cochlea
    Article Snippet: PCR was performed according to manufacturer's instructions using RT² SYBR Green ROX qPCR Mastermix (Qiagen) and RT² Profiler PCR Array Mouse Wnt Signaling Pathway (Qiagen, PAMM-043ZC) or RT2 Profiler PCR Array Mouse Notch Signaling pathway (Qiagen, PAMM-059ZC). .. Quality control, fold changes in gene expression and statistical analysis (two-tailed Student's t -test with the significance threshold set to P < 0.05) was performed using RT2 Profiler PCR Array Data Analysis Workbook, Version 4 (Qiagen, ).

    Derivative Assay:

    Article Title: Innate And Adaptive Immunity are Progressively Activated in Parallel with Renal Injury in the 5/6 Renal Ablation Model
    Article Snippet: For this, cDNA obtained from 9 animals of each experimental group was distributed among three pools, derived from 3 animals each. .. The cDNAs were mixed with the appropriate PCR master mix buffer (Rt² Syber Green ROX qPCR Primer Assay QiagenN® #330523) and analyzed on specific array plates (Rat Innate & Adaptive Immune Responses Rt² Profiler™ PCR, Qiagen®, #330231-PARN052Zc).

    Article Title: CD95 maintains stem cell-like and non-classical EMT programs in primary human glioblastoma cells
    Article Snippet: Later, cDNA was mixed with RT2 SYBR Green qPCR Mastermix (SABioscienceGermany — QIAGEN GmbH, Hilden, Germany Cat. No. 330522) and loaded on either 96-well or 384-well RT2 Profiler Epithelial to Mesenchymal Transition PCR Array plates (Qiagen, GmbH, Hilden, Germany Cat. No. PAHS-090Z; PAHS-090ZE-4). .. Expectations regarding an upregulation or downregulation in EMT were annotated by the manufacturer's guidelines for some of the genes in the array and was complemented by information derived from searching Pubmed for non-annotated genes.

    Generated:

    Article Title: Isolation of Cancer Stem Like Cells from Human Adenosquamous Carcinoma of the Lung Supports a Monoclonal Origin from a Multipotential Tissue Stem Cell
    Article Snippet: .. To evaluate expression of specific genes, RT-PCR reactions were performed on cDNA generated from total RNA isolated from the individual CSLC cultures using RT² qPCR Primer Assays and GAPDH (glyceraldehyde 3-phosphate dehydrogenase) as a constitutively active gene control, and RT² SYBR® Green/ROX qPCR Mastermix (Qiagen). .. Expression of selected genes was also determined in normal human lung and isolated normal human bronchial epithelial cells.

    Cell Culture:

    Article Title: Transcriptional response to Wnt activation regulates the regenerative capacity of the mammalian cochlea
    Article Snippet: Cochlear explants established on P0 were cultured with DMSO or 10 μM CHIR99021 for 5 DIV. .. PCR was performed according to manufacturer's instructions using RT² SYBR Green ROX qPCR Mastermix (Qiagen) and RT² Profiler PCR Array Mouse Wnt Signaling Pathway (Qiagen, PAMM-043ZC) or RT2 Profiler PCR Array Mouse Notch Signaling pathway (Qiagen, PAMM-059ZC).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Isolation of Cancer Stem Like Cells from Human Adenosquamous Carcinoma of the Lung Supports a Monoclonal Origin from a Multipotential Tissue Stem Cell
    Article Snippet: .. To evaluate expression of specific genes, RT-PCR reactions were performed on cDNA generated from total RNA isolated from the individual CSLC cultures using RT² qPCR Primer Assays and GAPDH (glyceraldehyde 3-phosphate dehydrogenase) as a constitutively active gene control, and RT² SYBR® Green/ROX qPCR Mastermix (Qiagen). .. Expression of selected genes was also determined in normal human lung and isolated normal human bronchial epithelial cells.

    Article Title: Immune characteristics correlating with HSV‐1 immune control and effect of squaric acid dibutyl ester on immune characteristics of subjects with frequent herpes labialis episodes. Immune characteristics correlating with hsv‐1 immune control and effect of squaric acid dibutyl ester on immune characteristics of subjects with frequent herpes labialis episodes
    Article Snippet: .. 2.6.2 qPCR qPCR was done with the Qiagen RT PCR profiler gene analysis kit (catalog number 330451) using RT2 Sybr Green Rox qPCR Mastermix (24) (Part 330523) and Custom RT2 PCR array plates (Ref. 330171). .. For this 675 μL 2× RT2 SYBR green mastermix, 51 μL pre‐AMP synthesis reaction, and 623 μL RNase free water were mixed, and then 25 μL of the mixture was added to 48 wells on the left or right half of the custom PCR plate.

    Affinity Magnetic Separation:

    Article Title: SP-A2 contributes to miRNA-mediated sex differences in response to oxidative stress: pro-inflammatory, anti-apoptotic, and anti-oxidant pathways are involved
    Article Snippet: Gene expression assays AMs were lysed by the addition of QIAzol Lysis Reagent (Qiagen). .. Real-time PCR was performed using RT2 SYBR Green ROX qPCR Mastermix (#330520, Qiagen) on a QuantStudio 12K Flex Real-Time PCR System (Applied Biosystems, Waltham, MA) at the Pennsylvania State University College of Medicine Genomic Core Facility.

    Sequencing:

    Article Title: Isolation of Cancer Stem Like Cells from Human Adenosquamous Carcinoma of the Lung Supports a Monoclonal Origin from a Multipotential Tissue Stem Cell
    Article Snippet: To evaluate expression of specific genes, RT-PCR reactions were performed on cDNA generated from total RNA isolated from the individual CSLC cultures using RT² qPCR Primer Assays and GAPDH (glyceraldehyde 3-phosphate dehydrogenase) as a constitutively active gene control, and RT² SYBR® Green/ROX qPCR Mastermix (Qiagen). .. Threshold Cycle (Ct) for each primer set was determined by running RT-PCR reactions in the ABI PRISM® 7000 Sequence Detection System (Life Technologies Corporation).

    Fluorescence:

    Article Title: Determinants of drug entry into the developing brain
    Article Snippet: Regulation of gene expression was measured using RT-qPCR and amplification detected with SYBR Green fluorescence. .. The final 10µl reaction volume contained 2µl 1/10 diluted cDNA and 8µL SYBR Green master mix (5µl Rt2 SYBR Green ROX qPCR master mix, [QIAGEN, Cat no 330529], 1µl each forward and reverse primer, 1µl RNase free water).

    Methylation:

    Article Title: The Tumor Suppressor Prostate Apoptosis Response-4 (Par-4) is Regulated by Mutant IDH1 and Kills Glioma Stem Cells
    Article Snippet: Paragraph title: Methylation assay ... The genomic DNAs were digested using the EpiTect Methyl II DNA Restriction Kit (Qiagen, Valencia, CA, Cat. No.335452) and real-time PCR was performed using RT SYBR Green ROX qPCR Mastermix (Qiagen, Valencia, CA, Cat. No 330520).

    Article Title: Effect of Normal Pregnancy Followed by Lactation on Long-Term Maternal Health in a Mouse Model
    Article Snippet: Paragraph title: Oxytocin Receptor Methylation Experiments ... Enzymes were inactivated, and the digested samples were used to seed quantitative PCR (qPCR) reactions using SYBR Green MasterMix (RT2 SYBR Green/ROX qPCR MasterMix, cat # 330520; Qiagen) and an Oxtr profiler primer.

    Isolation:

    Article Title: Isolation of Cancer Stem Like Cells from Human Adenosquamous Carcinoma of the Lung Supports a Monoclonal Origin from a Multipotential Tissue Stem Cell
    Article Snippet: .. To evaluate expression of specific genes, RT-PCR reactions were performed on cDNA generated from total RNA isolated from the individual CSLC cultures using RT² qPCR Primer Assays and GAPDH (glyceraldehyde 3-phosphate dehydrogenase) as a constitutively active gene control, and RT² SYBR® Green/ROX qPCR Mastermix (Qiagen). .. Expression of selected genes was also determined in normal human lung and isolated normal human bronchial epithelial cells.

    Article Title: Activation of Melatonin Signaling Promotes β-Cell Survival and Function
    Article Snippet: Subsequently, cells were homogenized using QIAshredder microcentrifuge spin-columns (79654; QIAGEN), and total RNA isolation was performed using the RNeasy mini kit (74104; QIAGEN) according to manufacturer's instructions. .. RNA abundance was quantified using the NanoDrop 1000 spectrophotometer (NanoDrop Technologies), and cDNA was synthesized from 0.5 μg total RNA using the RT2 first-strand kit (330520; QIAGEN) according to the manufacturer's instructions.

    Article Title: Responses of Multipotent Retinal Stem Cells to IL-1β, IL-18, or IL-17
    Article Snippet: Paragraph title: 2.6. RNA Isolation and Quantitative RT-PCR ... Quantitative RT-PCR (qRT-PCR) was performed using RT² SYBR Green ROX qPCR Mastermix (Qiagen). cDNA was amplified with primers β-actin , Il-6 , Il-17rc , or Il-17a (Qiagen) separately for 50 cycles.

    Purification:

    Article Title: Pathological Remodeling of Mitral Valve Leaflets from Unphysiologic Leaflet Mechanics after Undersized Mitral Annuloplasty to Repair Ischemic Mitral Regurgitation
    Article Snippet: Thirty milligrams of leaflet tissue from each pig was homogenized, and total RNA was extracted with RiboPure RNA Purification Kit (AM1924, Thermo Fisher), with the concentration and purity determined with a spectrophotometer (Synergy HT, BioTek). .. PCR components were mixed for each 96‐well plate: 1350 μL 2xRT SYBR Green Mastermix, 102 μL cDNA synthesis reaction and 1248 μL RNase‐free water (330523, Qiagen).

    Article Title: SP-A2 contributes to miRNA-mediated sex differences in response to oxidative stress: pro-inflammatory, anti-apoptotic, and anti-oxidant pathways are involved
    Article Snippet: Total RNA was purified with Direct-zol RNA Mini Prep kit (#R2052, Zymo Research, Irvine, CA), and RNA concentration and quality were confirmed by Nanodrop and Bioanalyzer. .. Real-time PCR was performed using RT2 SYBR Green ROX qPCR Mastermix (#330520, Qiagen) on a QuantStudio 12K Flex Real-Time PCR System (Applied Biosystems, Waltham, MA) at the Pennsylvania State University College of Medicine Genomic Core Facility.

    Polymerase Chain Reaction:

    Article Title: The Tumor Suppressor Prostate Apoptosis Response-4 (Par-4) is Regulated by Mutant IDH1 and Kills Glioma Stem Cells
    Article Snippet: Methylation assay was performed using EpiTect Methyl II PCR Assay (Qiagen, Valencia, CA, Cat. No. 335002) according the manufacturer's instructions. .. The genomic DNAs were digested using the EpiTect Methyl II DNA Restriction Kit (Qiagen, Valencia, CA, Cat. No.335452) and real-time PCR was performed using RT SYBR Green ROX qPCR Mastermix (Qiagen, Valencia, CA, Cat. No 330520).

    Article Title: TLR7 agonist administration to SIV-infected macaques receiving early initiated cART does not induce plasma viremia
    Article Snippet: .. Extracted RNA (150 ng per sample) was next used to generate cDNA using the RT2 First Strand Kit, with ISG sequences in the cDNA quantified by qPCR on an ABI 7500 real-timer PCR system using the Rhesus Macaque Type I Interferon Response RT² Profiler PCR Array with the RT² SYBR Green ROX qPCR Mastermix (Qiagen), all according to the manufacturers’ instructions. ..

    Article Title: Effect of Normal Pregnancy Followed by Lactation on Long-Term Maternal Health in a Mouse Model
    Article Snippet: EpiTect Methyl II PCR Primer Assay for mouse Oxtr (CpG Island 109491, located on chromosome 6: 112439018-112439877, cat # EPMM109491-1A; Qiagen) was used to assay methylation according to the manufacturer’s instructions. .. Enzymes were inactivated, and the digested samples were used to seed quantitative PCR (qPCR) reactions using SYBR Green MasterMix (RT2 SYBR Green/ROX qPCR MasterMix, cat # 330520; Qiagen) and an Oxtr profiler primer.

    Article Title: Pathological Remodeling of Mitral Valve Leaflets from Unphysiologic Leaflet Mechanics after Undersized Mitral Annuloplasty to Repair Ischemic Mitral Regurgitation
    Article Snippet: .. PCR components were mixed for each 96‐well plate: 1350 μL 2xRT SYBR Green Mastermix, 102 μL cDNA synthesis reaction and 1248 μL RNase‐free water (330523, Qiagen). .. Twenty‐five microliters of PCR components were added to each well, and the plates were centrifuged for 1 minute at 1000 g at room temperature.

    Article Title: Innate And Adaptive Immunity are Progressively Activated in Parallel with Renal Injury in the 5/6 Renal Ablation Model
    Article Snippet: .. The cDNAs were mixed with the appropriate PCR master mix buffer (Rt² Syber Green ROX qPCR Primer Assay QiagenN® #330523) and analyzed on specific array plates (Rat Innate & Adaptive Immune Responses Rt² Profiler™ PCR, Qiagen®, #330231-PARN052Zc). .. We further analyzed the expression of selected genes involved in the innate immune response by individual qPCR (10 animals/group).

    Article Title: CD95 maintains stem cell-like and non-classical EMT programs in primary human glioblastoma cells
    Article Snippet: .. Later, cDNA was mixed with RT2 SYBR Green qPCR Mastermix (SABioscienceGermany — QIAGEN GmbH, Hilden, Germany Cat. No. 330522) and loaded on either 96-well or 384-well RT2 Profiler Epithelial to Mesenchymal Transition PCR Array plates (Qiagen, GmbH, Hilden, Germany Cat. No. PAHS-090Z; PAHS-090ZE-4). .. The arrays were run as technical triplicates either on an ABI 7500 Real-Time PCR-System (Applied Bioscience/Thermo Fisher Scientific GmbH, Schwerte, Germany) for the 96-well plates or an CFX384 Real-Time PCR-System (Bio-Rad Laboratories GmbH, München, Germany) following the manufacturers' protocols, and the results for individual samples were averaged between the triplicates.

    Article Title: Isolation of Cancer Stem Like Cells from Human Adenosquamous Carcinoma of the Lung Supports a Monoclonal Origin from a Multipotential Tissue Stem Cell
    Article Snippet: Human DNA was quantified by PCR using human-specific RPL19 gene primers and probes [ ]. .. To evaluate expression of specific genes, RT-PCR reactions were performed on cDNA generated from total RNA isolated from the individual CSLC cultures using RT² qPCR Primer Assays and GAPDH (glyceraldehyde 3-phosphate dehydrogenase) as a constitutively active gene control, and RT² SYBR® Green/ROX qPCR Mastermix (Qiagen).

    Article Title: Determinants of drug entry into the developing brain
    Article Snippet: PCR efficiencies were validated. .. The final 10µl reaction volume contained 2µl 1/10 diluted cDNA and 8µL SYBR Green master mix (5µl Rt2 SYBR Green ROX qPCR master mix, [QIAGEN, Cat no 330529], 1µl each forward and reverse primer, 1µl RNase free water).

    Article Title: Molecular Mechanism Underlying Pathogenesis of Lewisite-Induced Cutaneous Blistering and Inflammation
    Article Snippet: .. Cignal Finder 45-Pathway Reporter Array plate (CCA-901L), RT2 First Strand kit (330401), RT2 qPCR Master Mix (330523), and mouse inflammatory cytokines and receptor PCR arrays (PA-011-12) were from Qiagen (Hilden, Germany). .. Dual-Glo Luciferase Assay System (E2940) and CellTiter 96 AQueous One Solution Cell Proliferation Assay (G3582) were from Promega (Madison, WI).

    Article Title: Activation of Melatonin Signaling Promotes β-Cell Survival and Function
    Article Snippet: Paragraph title: Targeted PCR arrays ... RNA abundance was quantified using the NanoDrop 1000 spectrophotometer (NanoDrop Technologies), and cDNA was synthesized from 0.5 μg total RNA using the RT2 first-strand kit (330520; QIAGEN) according to the manufacturer's instructions.

    Article Title: Immune characteristics correlating with HSV‐1 immune control and effect of squaric acid dibutyl ester on immune characteristics of subjects with frequent herpes labialis episodes. Immune characteristics correlating with hsv‐1 immune control and effect of squaric acid dibutyl ester on immune characteristics of subjects with frequent herpes labialis episodes
    Article Snippet: .. 2.6.2 qPCR qPCR was done with the Qiagen RT PCR profiler gene analysis kit (catalog number 330451) using RT2 Sybr Green Rox qPCR Mastermix (24) (Part 330523) and Custom RT2 PCR array plates (Ref. 330171). .. For this 675 μL 2× RT2 SYBR green mastermix, 51 μL pre‐AMP synthesis reaction, and 623 μL RNase free water were mixed, and then 25 μL of the mixture was added to 48 wells on the left or right half of the custom PCR plate.

    Article Title: Transcriptional response to Wnt activation regulates the regenerative capacity of the mammalian cochlea
    Article Snippet: .. PCR was performed according to manufacturer's instructions using RT² SYBR Green ROX qPCR Mastermix (Qiagen) and RT² Profiler PCR Array Mouse Wnt Signaling Pathway (Qiagen, PAMM-043ZC) or RT2 Profiler PCR Array Mouse Notch Signaling pathway (Qiagen, PAMM-059ZC). ..

    Chloramphenicol Acetyltransferase Assay:

    Article Title: SP-A2 contributes to miRNA-mediated sex differences in response to oxidative stress: pro-inflammatory, anti-apoptotic, and anti-oxidant pathways are involved
    Article Snippet: Real-time PCR was performed using RT2 SYBR Green ROX qPCR Mastermix (#330520, Qiagen) on a QuantStudio 12K Flex Real-Time PCR System (Applied Biosystems, Waltham, MA) at the Pennsylvania State University College of Medicine Genomic Core Facility. .. The expression levels of GAPDH, SOD2, CAT, IL-6, STAT3, BCL2, FOXO1, FOXO3, BECN1, IKKβ, and NF-kB-p65 were detected by qRT-PCR with specific RT2 qPCR Primer Assays (Qiagen).

    Plasmid Preparation:

    Article Title: The Tumor Suppressor Prostate Apoptosis Response-4 (Par-4) is Regulated by Mutant IDH1 and Kills Glioma Stem Cells
    Article Snippet: Briefly, U87 cells were harvested after treatment with or without 2-HG; cells stably expressing either vector-GFP or R132H IDH1-GFP were also harvested. .. The genomic DNAs were digested using the EpiTect Methyl II DNA Restriction Kit (Qiagen, Valencia, CA, Cat. No.335452) and real-time PCR was performed using RT SYBR Green ROX qPCR Mastermix (Qiagen, Valencia, CA, Cat. No 330520).

    Software:

    Article Title: The Tumor Suppressor Prostate Apoptosis Response-4 (Par-4) is Regulated by Mutant IDH1 and Kills Glioma Stem Cells
    Article Snippet: The genomic DNAs were digested using the EpiTect Methyl II DNA Restriction Kit (Qiagen, Valencia, CA, Cat. No.335452) and real-time PCR was performed using RT SYBR Green ROX qPCR Mastermix (Qiagen, Valencia, CA, Cat. No 330520). .. The data was analyzed using the methylation assay software provided by Qiagen.

    Article Title: Innate And Adaptive Immunity are Progressively Activated in Parallel with Renal Injury in the 5/6 Renal Ablation Model
    Article Snippet: The cDNAs were mixed with the appropriate PCR master mix buffer (Rt² Syber Green ROX qPCR Primer Assay QiagenN® #330523) and analyzed on specific array plates (Rat Innate & Adaptive Immune Responses Rt² Profiler™ PCR, Qiagen®, #330231-PARN052Zc). .. Both PCR-array and qPCR analysis were performed using a ViiA™7 Real-Time PCR System (Life Technologies do Brasil, São Paulo) and the results assessed with the 12 K Flex QuantStudioTM software (Applied Biosystems, Foster City, CA).

    Article Title: Activation of Melatonin Signaling Promotes β-Cell Survival and Function
    Article Snippet: RNA abundance was quantified using the NanoDrop 1000 spectrophotometer (NanoDrop Technologies), and cDNA was synthesized from 0.5 μg total RNA using the RT2 first-strand kit (330520; QIAGEN) according to the manufacturer's instructions. .. Free web-based RT2 Profiler PCR Array Data Analysis Software (version 3.5; SABiosciences) was used to determine δδcycle threshold based fold-changes in expression of target genes using standardized settings (ie, baseline, threshold) and β-actin as the endogenous control gene across all plates.

    Real-time Polymerase Chain Reaction:

    Article Title: The Tumor Suppressor Prostate Apoptosis Response-4 (Par-4) is Regulated by Mutant IDH1 and Kills Glioma Stem Cells
    Article Snippet: .. The genomic DNAs were digested using the EpiTect Methyl II DNA Restriction Kit (Qiagen, Valencia, CA, Cat. No.335452) and real-time PCR was performed using RT SYBR Green ROX qPCR Mastermix (Qiagen, Valencia, CA, Cat. No 330520). .. The data was analyzed using the methylation assay software provided by Qiagen.

    Article Title: TLR7 agonist administration to SIV-infected macaques receiving early initiated cART does not induce plasma viremia
    Article Snippet: .. Extracted RNA (150 ng per sample) was next used to generate cDNA using the RT2 First Strand Kit, with ISG sequences in the cDNA quantified by qPCR on an ABI 7500 real-timer PCR system using the Rhesus Macaque Type I Interferon Response RT² Profiler PCR Array with the RT² SYBR Green ROX qPCR Mastermix (Qiagen), all according to the manufacturers’ instructions. ..

    Article Title: Effect of Normal Pregnancy Followed by Lactation on Long-Term Maternal Health in a Mouse Model
    Article Snippet: .. Enzymes were inactivated, and the digested samples were used to seed quantitative PCR (qPCR) reactions using SYBR Green MasterMix (RT2 SYBR Green/ROX qPCR MasterMix, cat # 330520; Qiagen) and an Oxtr profiler primer. .. Changes in critical threshold values from the qPCR reaction were analyzed using a methyl profiler data analysis template and reported as percentages of methylation of samples run in triplicate.

    Article Title: Pathological Remodeling of Mitral Valve Leaflets from Unphysiologic Leaflet Mechanics after Undersized Mitral Annuloplasty to Repair Ischemic Mitral Regurgitation
    Article Snippet: PCR components were mixed for each 96‐well plate: 1350 μL 2xRT SYBR Green Mastermix, 102 μL cDNA synthesis reaction and 1248 μL RNase‐free water (330523, Qiagen). .. Real‐time quantitative PCR reaction was performed in a 96‐well plate array consisting of pig extracellular matrix (ECM) genes, adhesion genes and fibrosis pathway genes.

    Article Title: Innate And Adaptive Immunity are Progressively Activated in Parallel with Renal Injury in the 5/6 Renal Ablation Model
    Article Snippet: .. The cDNAs were mixed with the appropriate PCR master mix buffer (Rt² Syber Green ROX qPCR Primer Assay QiagenN® #330523) and analyzed on specific array plates (Rat Innate & Adaptive Immune Responses Rt² Profiler™ PCR, Qiagen®, #330231-PARN052Zc). .. We further analyzed the expression of selected genes involved in the innate immune response by individual qPCR (10 animals/group).

    Article Title: CD95 maintains stem cell-like and non-classical EMT programs in primary human glioblastoma cells
    Article Snippet: .. Later, cDNA was mixed with RT2 SYBR Green qPCR Mastermix (SABioscienceGermany — QIAGEN GmbH, Hilden, Germany Cat. No. 330522) and loaded on either 96-well or 384-well RT2 Profiler Epithelial to Mesenchymal Transition PCR Array plates (Qiagen, GmbH, Hilden, Germany Cat. No. PAHS-090Z; PAHS-090ZE-4). .. The arrays were run as technical triplicates either on an ABI 7500 Real-Time PCR-System (Applied Bioscience/Thermo Fisher Scientific GmbH, Schwerte, Germany) for the 96-well plates or an CFX384 Real-Time PCR-System (Bio-Rad Laboratories GmbH, München, Germany) following the manufacturers' protocols, and the results for individual samples were averaged between the triplicates.

    Article Title: Isolation of Cancer Stem Like Cells from Human Adenosquamous Carcinoma of the Lung Supports a Monoclonal Origin from a Multipotential Tissue Stem Cell
    Article Snippet: .. To evaluate expression of specific genes, RT-PCR reactions were performed on cDNA generated from total RNA isolated from the individual CSLC cultures using RT² qPCR Primer Assays and GAPDH (glyceraldehyde 3-phosphate dehydrogenase) as a constitutively active gene control, and RT² SYBR® Green/ROX qPCR Mastermix (Qiagen). .. Expression of selected genes was also determined in normal human lung and isolated normal human bronchial epithelial cells.

    Article Title: SP-A2 contributes to miRNA-mediated sex differences in response to oxidative stress: pro-inflammatory, anti-apoptotic, and anti-oxidant pathways are involved
    Article Snippet: .. Real-time PCR was performed using RT2 SYBR Green ROX qPCR Mastermix (#330520, Qiagen) on a QuantStudio 12K Flex Real-Time PCR System (Applied Biosystems, Waltham, MA) at the Pennsylvania State University College of Medicine Genomic Core Facility. .. The expression levels of GAPDH, SOD2, CAT, IL-6, STAT3, BCL2, FOXO1, FOXO3, BECN1, IKKβ, and NF-kB-p65 were detected by qRT-PCR with specific RT2 qPCR Primer Assays (Qiagen).

    Article Title: Determinants of drug entry into the developing brain
    Article Snippet: .. The final 10µl reaction volume contained 2µl 1/10 diluted cDNA and 8µL SYBR Green master mix (5µl Rt2 SYBR Green ROX qPCR master mix, [QIAGEN, Cat no 330529], 1µl each forward and reverse primer, 1µl RNase free water). ..

    Article Title: Molecular Mechanism Underlying Pathogenesis of Lewisite-Induced Cutaneous Blistering and Inflammation
    Article Snippet: .. Cignal Finder 45-Pathway Reporter Array plate (CCA-901L), RT2 First Strand kit (330401), RT2 qPCR Master Mix (330523), and mouse inflammatory cytokines and receptor PCR arrays (PA-011-12) were from Qiagen (Hilden, Germany). .. Dual-Glo Luciferase Assay System (E2940) and CellTiter 96 AQueous One Solution Cell Proliferation Assay (G3582) were from Promega (Madison, WI).

    Article Title: Activation of Melatonin Signaling Promotes β-Cell Survival and Function
    Article Snippet: RNA abundance was quantified using the NanoDrop 1000 spectrophotometer (NanoDrop Technologies), and cDNA was synthesized from 0.5 μg total RNA using the RT2 first-strand kit (330520; QIAGEN) according to the manufacturer's instructions. .. The following targeted PCR arrays were performed in 96-well format using the RT2 SYBR Green ROX quantitative PCR master mix (330520; QIAGEN) and the QuantStudio 6 Flex real-time PCR system (Applied Biosystems) according to the manufacturer's instructions: rat apoptosis RT2 Profiler PCR array (PARN-012ZC-2; QIAGEN) and rat oxidative stress RT2 profiler PCR array (PARN-065ZC-2; QIAGEN).

    Article Title: Responses of Multipotent Retinal Stem Cells to IL-1β, IL-18, or IL-17
    Article Snippet: .. Quantitative RT-PCR (qRT-PCR) was performed using RT² SYBR Green ROX qPCR Mastermix (Qiagen). cDNA was amplified with primers β-actin , Il-6 , Il-17rc , or Il-17a (Qiagen) separately for 50 cycles. ..

    Article Title: Immune characteristics correlating with HSV‐1 immune control and effect of squaric acid dibutyl ester on immune characteristics of subjects with frequent herpes labialis episodes. Immune characteristics correlating with hsv‐1 immune control and effect of squaric acid dibutyl ester on immune characteristics of subjects with frequent herpes labialis episodes
    Article Snippet: .. 2.6.2 qPCR qPCR was done with the Qiagen RT PCR profiler gene analysis kit (catalog number 330451) using RT2 Sybr Green Rox qPCR Mastermix (24) (Part 330523) and Custom RT2 PCR array plates (Ref. 330171). .. For this 675 μL 2× RT2 SYBR green mastermix, 51 μL pre‐AMP synthesis reaction, and 623 μL RNase free water were mixed, and then 25 μL of the mixture was added to 48 wells on the left or right half of the custom PCR plate.

    Article Title: Transcriptional response to Wnt activation regulates the regenerative capacity of the mammalian cochlea
    Article Snippet: .. PCR was performed according to manufacturer's instructions using RT² SYBR Green ROX qPCR Mastermix (Qiagen) and RT² Profiler PCR Array Mouse Wnt Signaling Pathway (Qiagen, PAMM-043ZC) or RT2 Profiler PCR Array Mouse Notch Signaling pathway (Qiagen, PAMM-059ZC). ..

    In Situ:

    Article Title: Molecular Mechanism Underlying Pathogenesis of Lewisite-Induced Cutaneous Blistering and Inflammation
    Article Snippet: Cignal Finder 45-Pathway Reporter Array plate (CCA-901L), RT2 First Strand kit (330401), RT2 qPCR Master Mix (330523), and mouse inflammatory cytokines and receptor PCR arrays (PA-011-12) were from Qiagen (Hilden, Germany). .. In situ Cell Death Detection Kit (1684795) was from Roche (Indianapolis, IN).

    Enzyme-linked Immunosorbent Assay:

    Article Title: Molecular Mechanism Underlying Pathogenesis of Lewisite-Induced Cutaneous Blistering and Inflammation
    Article Snippet: Cignal Finder 45-Pathway Reporter Array plate (CCA-901L), RT2 First Strand kit (330401), RT2 qPCR Master Mix (330523), and mouse inflammatory cytokines and receptor PCR arrays (PA-011-12) were from Qiagen (Hilden, Germany). .. Prostaglandin E2 (PGE2 ) Express Enzyme Immunoassay Kit (500141) was from Cayman Chemical.

    Spectrophotometry:

    Article Title: Pathological Remodeling of Mitral Valve Leaflets from Unphysiologic Leaflet Mechanics after Undersized Mitral Annuloplasty to Repair Ischemic Mitral Regurgitation
    Article Snippet: Thirty milligrams of leaflet tissue from each pig was homogenized, and total RNA was extracted with RiboPure RNA Purification Kit (AM1924, Thermo Fisher), with the concentration and purity determined with a spectrophotometer (Synergy HT, BioTek). .. PCR components were mixed for each 96‐well plate: 1350 μL 2xRT SYBR Green Mastermix, 102 μL cDNA synthesis reaction and 1248 μL RNase‐free water (330523, Qiagen).

    Article Title: Activation of Melatonin Signaling Promotes β-Cell Survival and Function
    Article Snippet: .. RNA abundance was quantified using the NanoDrop 1000 spectrophotometer (NanoDrop Technologies), and cDNA was synthesized from 0.5 μg total RNA using the RT2 first-strand kit (330520; QIAGEN) according to the manufacturer's instructions. .. The following targeted PCR arrays were performed in 96-well format using the RT2 SYBR Green ROX quantitative PCR master mix (330520; QIAGEN) and the QuantStudio 6 Flex real-time PCR system (Applied Biosystems) according to the manufacturer's instructions: rat apoptosis RT2 Profiler PCR array (PARN-012ZC-2; QIAGEN) and rat oxidative stress RT2 profiler PCR array (PARN-065ZC-2; QIAGEN).

    Produced:

    Article Title: Determinants of drug entry into the developing brain
    Article Snippet: The final 10µl reaction volume contained 2µl 1/10 diluted cDNA and 8µL SYBR Green master mix (5µl Rt2 SYBR Green ROX qPCR master mix, [QIAGEN, Cat no 330529], 1µl each forward and reverse primer, 1µl RNase free water). .. NTCs produced no signal, or the occasional negligible signal ( > 38Ct).

    Concentration Assay:

    Article Title: Pathological Remodeling of Mitral Valve Leaflets from Unphysiologic Leaflet Mechanics after Undersized Mitral Annuloplasty to Repair Ischemic Mitral Regurgitation
    Article Snippet: Thirty milligrams of leaflet tissue from each pig was homogenized, and total RNA was extracted with RiboPure RNA Purification Kit (AM1924, Thermo Fisher), with the concentration and purity determined with a spectrophotometer (Synergy HT, BioTek). .. PCR components were mixed for each 96‐well plate: 1350 μL 2xRT SYBR Green Mastermix, 102 μL cDNA synthesis reaction and 1248 μL RNase‐free water (330523, Qiagen).

    Article Title: Innate And Adaptive Immunity are Progressively Activated in Parallel with Renal Injury in the 5/6 Renal Ablation Model
    Article Snippet: After evaluation of RNA purity and final concentration, cDNA was synthesized through reverse transcription (RT) using the Rt² Easy First Strand kit (Qiagen® #330401). .. The cDNAs were mixed with the appropriate PCR master mix buffer (Rt² Syber Green ROX qPCR Primer Assay QiagenN® #330523) and analyzed on specific array plates (Rat Innate & Adaptive Immune Responses Rt² Profiler™ PCR, Qiagen®, #330231-PARN052Zc).

    Article Title: SP-A2 contributes to miRNA-mediated sex differences in response to oxidative stress: pro-inflammatory, anti-apoptotic, and anti-oxidant pathways are involved
    Article Snippet: Total RNA was purified with Direct-zol RNA Mini Prep kit (#R2052, Zymo Research, Irvine, CA), and RNA concentration and quality were confirmed by Nanodrop and Bioanalyzer. .. Real-time PCR was performed using RT2 SYBR Green ROX qPCR Mastermix (#330520, Qiagen) on a QuantStudio 12K Flex Real-Time PCR System (Applied Biosystems, Waltham, MA) at the Pennsylvania State University College of Medicine Genomic Core Facility.

    Article Title: Transcriptional response to Wnt activation regulates the regenerative capacity of the mammalian cochlea
    Article Snippet: Concentration of RNA was determined using a Qubit Fluorometer (Thermo Fisher Scientific) and cDNA was prepared using the RT2 First Strand Kit (Qiagen). .. PCR was performed according to manufacturer's instructions using RT² SYBR Green ROX qPCR Mastermix (Qiagen) and RT² Profiler PCR Array Mouse Wnt Signaling Pathway (Qiagen, PAMM-043ZC) or RT2 Profiler PCR Array Mouse Notch Signaling pathway (Qiagen, PAMM-059ZC).

    CpG Assay:

    Article Title: The Tumor Suppressor Prostate Apoptosis Response-4 (Par-4) is Regulated by Mutant IDH1 and Kills Glioma Stem Cells
    Article Snippet: The human PAWR CpG assay (Cat. No. PM00160664) was purchased from Qiagen (Valencia, CA). .. The genomic DNAs were digested using the EpiTect Methyl II DNA Restriction Kit (Qiagen, Valencia, CA, Cat. No.335452) and real-time PCR was performed using RT SYBR Green ROX qPCR Mastermix (Qiagen, Valencia, CA, Cat. No 330520).

    DNA Purification:

    Article Title: Isolation of Cancer Stem Like Cells from Human Adenosquamous Carcinoma of the Lung Supports a Monoclonal Origin from a Multipotential Tissue Stem Cell
    Article Snippet: Reverse transcriptase (RT-PCR) analysis DNA was isolated from animal samples using the Wizard SV Genomic DNA Purification kit following the manufacturer’s protocol (Promega). .. To evaluate expression of specific genes, RT-PCR reactions were performed on cDNA generated from total RNA isolated from the individual CSLC cultures using RT² qPCR Primer Assays and GAPDH (glyceraldehyde 3-phosphate dehydrogenase) as a constitutively active gene control, and RT² SYBR® Green/ROX qPCR Mastermix (Qiagen).

    Lysis:

    Article Title: Innate And Adaptive Immunity are Progressively Activated in Parallel with Renal Injury in the 5/6 Renal Ablation Model
    Article Snippet: Kidney samples were placed in reinforced polypropylene tubes containing ceramic beads (Omni Bead Ruptor tubes #19-628) and the appropriate lysis buffer. .. The cDNAs were mixed with the appropriate PCR master mix buffer (Rt² Syber Green ROX qPCR Primer Assay QiagenN® #330523) and analyzed on specific array plates (Rat Innate & Adaptive Immune Responses Rt² Profiler™ PCR, Qiagen®, #330231-PARN052Zc).

    Article Title: SP-A2 contributes to miRNA-mediated sex differences in response to oxidative stress: pro-inflammatory, anti-apoptotic, and anti-oxidant pathways are involved
    Article Snippet: Gene expression assays AMs were lysed by the addition of QIAzol Lysis Reagent (Qiagen). .. Real-time PCR was performed using RT2 SYBR Green ROX qPCR Mastermix (#330520, Qiagen) on a QuantStudio 12K Flex Real-Time PCR System (Applied Biosystems, Waltham, MA) at the Pennsylvania State University College of Medicine Genomic Core Facility.

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  • 90
    Qiagen rt² sybr green qpcr mastermix
    Rt² Sybr Green Qpcr Mastermix, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rt² sybr green qpcr mastermix/product/Qiagen
    Average 90 stars, based on 37 article reviews
    Price from $9.99 to $1999.99
    rt² sybr green qpcr mastermix - by Bioz Stars, 2020-01
    90/100 stars
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    90
    Qiagen rt² sybr green rox qpcr mastermix
    Rt² Sybr Green Rox Qpcr Mastermix, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rt² sybr green rox qpcr mastermix/product/Qiagen
    Average 90 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    rt² sybr green rox qpcr mastermix - by Bioz Stars, 2020-01
    90/100 stars
      Buy from Supplier

    N/A
    For recreating Screening Suite conditions Kit contents 200 ml 0 05M Hexadecyltrimethylammonium bromide
      Buy from Supplier

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