rt² profiler pcr array  (Qiagen)


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    Name:
    RT² Profiler PCR Array
    Description:
    RT² Profiler PCR Arrays enable quick reliable gene expression analysis of 170 pathways allowing researchers to spend less time at the bench and more time interpreting results The arrays are pathway focused panels of laboratory verified qPCR assays with integrated patented controls to ensure a successful experiment every time PhD trained application specialists are available to provide technical support for the arrays and each array can also be modified to suit unique experimental needs For biomarker discoveries we offer Pathway Plus PCR arrays and all arrays listed below for the Fluidigm BioMark Real Time PCR System
    Catalog Number:
    330231
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    Category:
    Assay PCR qPCR
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    Structured Review

    Qiagen rt² profiler pcr array
    RT² Profiler PCR Array
    RT² Profiler PCR Arrays enable quick reliable gene expression analysis of 170 pathways allowing researchers to spend less time at the bench and more time interpreting results The arrays are pathway focused panels of laboratory verified qPCR assays with integrated patented controls to ensure a successful experiment every time PhD trained application specialists are available to provide technical support for the arrays and each array can also be modified to suit unique experimental needs For biomarker discoveries we offer Pathway Plus PCR arrays and all arrays listed below for the Fluidigm BioMark Real Time PCR System
    https://www.bioz.com/result/rt² profiler pcr array/product/Qiagen
    Average 90 stars, based on 391 article reviews
    Price from $9.99 to $1999.99
    rt² profiler pcr array - by Bioz Stars, 2020-01
    90/100 stars

    Images

    1) Product Images from "Doxycycline Enhances Survival and Self-Renewal of Human Pluripotent Stem Cells"

    Article Title: Doxycycline Enhances Survival and Self-Renewal of Human Pluripotent Stem Cells

    Journal: Stem Cell Reports

    doi: 10.1016/j.stemcr.2014.06.013

    Practical Benefits of Doxycycline Supplementation in hESC/iPSC Cultures The effects of doxycycline supplementation were examined in undifferentiated hESC/iPSC cultures maintained in clusters without dissociation on MEF feeder cells. (A and B) FACS analyses for determining the fraction of cells undergoing apoptosis (A) and the fraction that had accumulated in each cell-cycle phase (B). Small clusters of H9 hESCs or Lenti-1 hiPSCs (10-50 cells) were plated and cultured in the presence or absence of doxycycline (1 μg/ml) for 6 days. The hESC/iPSC clusters were mechanically harvested, dissociated using TrypLE (A) or Accutase (B), 2 hr prior to Annexin V/PI (A) or PI (B) staining. The stained populations were analyzed using FACS. n = 2 or 3 technical replicates/experiment, n = 2 experiments (A). n = 3 experiments (B) ∗ p
    Figure Legend Snippet: Practical Benefits of Doxycycline Supplementation in hESC/iPSC Cultures The effects of doxycycline supplementation were examined in undifferentiated hESC/iPSC cultures maintained in clusters without dissociation on MEF feeder cells. (A and B) FACS analyses for determining the fraction of cells undergoing apoptosis (A) and the fraction that had accumulated in each cell-cycle phase (B). Small clusters of H9 hESCs or Lenti-1 hiPSCs (10-50 cells) were plated and cultured in the presence or absence of doxycycline (1 μg/ml) for 6 days. The hESC/iPSC clusters were mechanically harvested, dissociated using TrypLE (A) or Accutase (B), 2 hr prior to Annexin V/PI (A) or PI (B) staining. The stained populations were analyzed using FACS. n = 2 or 3 technical replicates/experiment, n = 2 experiments (A). n = 3 experiments (B) ∗ p

    Techniques Used: FACS, Cell Culture, Staining

    Doxycycline Permits Cell Survival and Maintenance of Undifferentiated hESCs through PI3K-AKT Signal Activation. (A–C) Cell dissociation-induced actomyosin hyperactivation is prevented by Y-27632, but not by doxycycline treatment. H9 hESCs were incubated with doxycycline or Y-27632 or without these chemicals (control) for 1 hr prior to cell dissociation. Cells were then harvested 0, 10, and 30 min after cell dissociation and subjected to western blot analyses to detect proteins involved in cell-dissociation-induced apoptosis (A). Shown in (B) and (C) are representative pMLC-immunostained and phase-contrast images of dissociated hESCs in the cultures treated with Y-27632 or doxycycline. Cells pretreated with Y-27632 or doxycycline were dissociated, directly plated onto Matrigel with mTeSR1 and cultured in the continuous presence of the chemicals. The images were captured at the indicated time after plating (dissociation). Scale bars, 30 μm. (D) Estimation of intracellular signals activated by doxycycline. H9 hESCs were incubated without (control) or with doxycycline (1 μg/ml) for 30 min before harvesting and then subjected to immunoblot analyses using the human phosphokinase blot array, which is designed to detect 46 phosphorylated intracellular proteins. The array analyses were performed in technical triplicate. Shown is a representative pair of blots of the untreated control and doxycycline-treated cells. In the blots, spots whose intensities are greater ( > 1.5-fold) in the doxycycline-treated group, compared to the control, are marked with dotted circles and listed with the fold increase (parentheses) in the inset. ∗∗ p
    Figure Legend Snippet: Doxycycline Permits Cell Survival and Maintenance of Undifferentiated hESCs through PI3K-AKT Signal Activation. (A–C) Cell dissociation-induced actomyosin hyperactivation is prevented by Y-27632, but not by doxycycline treatment. H9 hESCs were incubated with doxycycline or Y-27632 or without these chemicals (control) for 1 hr prior to cell dissociation. Cells were then harvested 0, 10, and 30 min after cell dissociation and subjected to western blot analyses to detect proteins involved in cell-dissociation-induced apoptosis (A). Shown in (B) and (C) are representative pMLC-immunostained and phase-contrast images of dissociated hESCs in the cultures treated with Y-27632 or doxycycline. Cells pretreated with Y-27632 or doxycycline were dissociated, directly plated onto Matrigel with mTeSR1 and cultured in the continuous presence of the chemicals. The images were captured at the indicated time after plating (dissociation). Scale bars, 30 μm. (D) Estimation of intracellular signals activated by doxycycline. H9 hESCs were incubated without (control) or with doxycycline (1 μg/ml) for 30 min before harvesting and then subjected to immunoblot analyses using the human phosphokinase blot array, which is designed to detect 46 phosphorylated intracellular proteins. The array analyses were performed in technical triplicate. Shown is a representative pair of blots of the untreated control and doxycycline-treated cells. In the blots, spots whose intensities are greater ( > 1.5-fold) in the doxycycline-treated group, compared to the control, are marked with dotted circles and listed with the fold increase (parentheses) in the inset. ∗∗ p

    Techniques Used: Activation Assay, Incubation, Western Blot, Cell Culture

    2) Product Images from "Doxycycline Enhances Survival and Self-Renewal of Human Pluripotent Stem Cells"

    Article Title: Doxycycline Enhances Survival and Self-Renewal of Human Pluripotent Stem Cells

    Journal: Stem Cell Reports

    doi: 10.1016/j.stemcr.2014.06.013

    Practical Benefits of Doxycycline Supplementation in hESC/iPSC Cultures The effects of doxycycline supplementation were examined in undifferentiated hESC/iPSC cultures maintained in clusters without dissociation on MEF feeder cells. (A and B) FACS analyses for determining the fraction of cells undergoing apoptosis (A) and the fraction that had accumulated in each cell-cycle phase (B). Small clusters of H9 hESCs or Lenti-1 hiPSCs (10-50 cells) were plated and cultured in the presence or absence of doxycycline (1 μg/ml) for 6 days. The hESC/iPSC clusters were mechanically harvested, dissociated using TrypLE (A) or Accutase (B), 2 hr prior to Annexin V/PI (A) or PI (B) staining. The stained populations were analyzed using FACS. n = 2 or 3 technical replicates/experiment, n = 2 experiments (A). n = 3 experiments (B) ∗ p
    Figure Legend Snippet: Practical Benefits of Doxycycline Supplementation in hESC/iPSC Cultures The effects of doxycycline supplementation were examined in undifferentiated hESC/iPSC cultures maintained in clusters without dissociation on MEF feeder cells. (A and B) FACS analyses for determining the fraction of cells undergoing apoptosis (A) and the fraction that had accumulated in each cell-cycle phase (B). Small clusters of H9 hESCs or Lenti-1 hiPSCs (10-50 cells) were plated and cultured in the presence or absence of doxycycline (1 μg/ml) for 6 days. The hESC/iPSC clusters were mechanically harvested, dissociated using TrypLE (A) or Accutase (B), 2 hr prior to Annexin V/PI (A) or PI (B) staining. The stained populations were analyzed using FACS. n = 2 or 3 technical replicates/experiment, n = 2 experiments (A). n = 3 experiments (B) ∗ p

    Techniques Used: FACS, Cell Culture, Staining

    Doxycycline Permits Cell Survival and Maintenance of Undifferentiated hESCs through PI3K-AKT Signal Activation. (A–C) Cell dissociation-induced actomyosin hyperactivation is prevented by Y-27632, but not by doxycycline treatment. H9 hESCs were incubated with doxycycline or Y-27632 or without these chemicals (control) for 1 hr prior to cell dissociation. Cells were then harvested 0, 10, and 30 min after cell dissociation and subjected to western blot analyses to detect proteins involved in cell-dissociation-induced apoptosis (A). Shown in (B) and (C) are representative pMLC-immunostained and phase-contrast images of dissociated hESCs in the cultures treated with Y-27632 or doxycycline. Cells pretreated with Y-27632 or doxycycline were dissociated, directly plated onto Matrigel with mTeSR1 and cultured in the continuous presence of the chemicals. The images were captured at the indicated time after plating (dissociation). Scale bars, 30 μm. (D) Estimation of intracellular signals activated by doxycycline. H9 hESCs were incubated without (control) or with doxycycline (1 μg/ml) for 30 min before harvesting and then subjected to immunoblot analyses using the human phosphokinase blot array, which is designed to detect 46 phosphorylated intracellular proteins. The array analyses were performed in technical triplicate. Shown is a representative pair of blots of the untreated control and doxycycline-treated cells. In the blots, spots whose intensities are greater ( > 1.5-fold) in the doxycycline-treated group, compared to the control, are marked with dotted circles and listed with the fold increase (parentheses) in the inset. ∗∗ p
    Figure Legend Snippet: Doxycycline Permits Cell Survival and Maintenance of Undifferentiated hESCs through PI3K-AKT Signal Activation. (A–C) Cell dissociation-induced actomyosin hyperactivation is prevented by Y-27632, but not by doxycycline treatment. H9 hESCs were incubated with doxycycline or Y-27632 or without these chemicals (control) for 1 hr prior to cell dissociation. Cells were then harvested 0, 10, and 30 min after cell dissociation and subjected to western blot analyses to detect proteins involved in cell-dissociation-induced apoptosis (A). Shown in (B) and (C) are representative pMLC-immunostained and phase-contrast images of dissociated hESCs in the cultures treated with Y-27632 or doxycycline. Cells pretreated with Y-27632 or doxycycline were dissociated, directly plated onto Matrigel with mTeSR1 and cultured in the continuous presence of the chemicals. The images were captured at the indicated time after plating (dissociation). Scale bars, 30 μm. (D) Estimation of intracellular signals activated by doxycycline. H9 hESCs were incubated without (control) or with doxycycline (1 μg/ml) for 30 min before harvesting and then subjected to immunoblot analyses using the human phosphokinase blot array, which is designed to detect 46 phosphorylated intracellular proteins. The array analyses were performed in technical triplicate. Shown is a representative pair of blots of the untreated control and doxycycline-treated cells. In the blots, spots whose intensities are greater ( > 1.5-fold) in the doxycycline-treated group, compared to the control, are marked with dotted circles and listed with the fold increase (parentheses) in the inset. ∗∗ p

    Techniques Used: Activation Assay, Incubation, Western Blot, Cell Culture

    3) Product Images from "Adipocyte arrestin domain-containing 3 protein (Arrdc3) regulates uncoupling protein 1 (Ucp1) expression in white adipose independently of canonical changes in β-adrenergic receptor signaling"

    Article Title: Adipocyte arrestin domain-containing 3 protein (Arrdc3) regulates uncoupling protein 1 (Ucp1) expression in white adipose independently of canonical changes in β-adrenergic receptor signaling

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0173823

    Arrdc3 deletion increases PPAR target gene expression in adipocytes. Quantitative PCR array analysis of gene expression of PPAR target genes, PPAR cofactors and PPARs and associated transcription factors in control versus Arrdc3 SVF-derived adipocytes in vitro (n = 3 mice per group). Only significantly different genes are displayed. *p≤ 0.05.
    Figure Legend Snippet: Arrdc3 deletion increases PPAR target gene expression in adipocytes. Quantitative PCR array analysis of gene expression of PPAR target genes, PPAR cofactors and PPARs and associated transcription factors in control versus Arrdc3 SVF-derived adipocytes in vitro (n = 3 mice per group). Only significantly different genes are displayed. *p≤ 0.05.

    Techniques Used: Expressing, Real-time Polymerase Chain Reaction, Derivative Assay, In Vitro, Mouse Assay

    4) Product Images from "CHAC2, downregulated in gastric and colorectal cancers, acted as a tumor suppressor inducing apoptosis and autophagy through unfolded protein response"

    Article Title: CHAC2, downregulated in gastric and colorectal cancers, acted as a tumor suppressor inducing apoptosis and autophagy through unfolded protein response

    Journal: Cell Death & Disease

    doi: 10.1038/cddis.2017.405

    Analysis of CHAC2 expression and its degradation pathway in gastric and colorectal cancer cell lines. ( a ) The expression of CHAC2 in gastric and colorectal cancer cell lines was determined by RT-PCR and western bolt, using GAPDH as a control. ( b ) CHAC2 expression in stably transfected cells confirmed by RT-PCR and western blot. ( c ) Evaluation of CHAC2 expression after treatment of 5-FU or BFA. ( d ) Analysis of the potential degradation pathway of CHAC2 using different inhibitors. AGS-CHAC2 and SW620-CHAC2 cells were treated with 20 μ g/ml CHX with or without MG-132 (20 μ M) or Leu (100 μ M) or CQ (50 mM) in a time course. Cell lysates were subjected to western blot with primary antibodies including CHAC2, p-ERK and GAPDH. ( e ) The effect of ubiquitin on CHAC2 expression. AGS-CHAC2 and SW620-CHAC2 cells were incubated with indicated rhHA-ubiquitin for 12 h, and cell lysates were subjected to western blot with primary antibodies including CHAC2, HA tag andGAPDH. ( f ) 12 maximal upregulated genes checked by the Human Ubiquitination Pathway RT 2 Profiler PCR Array were shown in the bar graph. Among these genes, RNF148 expression significantly increased nearly 1863 times in SW620 as compared to SW48. ( g ) The expression of RNF148 in gastric and colorectal cancer cell lines was determined by RT-PCR. ( h ) Evaluation of RNF148 expression after treatment of 5-FU or BFA. 5-FU, 5-fluorouracil; BFA, Brefeldin A; CHX, Cycloheximide; Leu, leupeptin; CQ, Chloroquine; Ubi, rhHA-ubiquitin
    Figure Legend Snippet: Analysis of CHAC2 expression and its degradation pathway in gastric and colorectal cancer cell lines. ( a ) The expression of CHAC2 in gastric and colorectal cancer cell lines was determined by RT-PCR and western bolt, using GAPDH as a control. ( b ) CHAC2 expression in stably transfected cells confirmed by RT-PCR and western blot. ( c ) Evaluation of CHAC2 expression after treatment of 5-FU or BFA. ( d ) Analysis of the potential degradation pathway of CHAC2 using different inhibitors. AGS-CHAC2 and SW620-CHAC2 cells were treated with 20 μ g/ml CHX with or without MG-132 (20 μ M) or Leu (100 μ M) or CQ (50 mM) in a time course. Cell lysates were subjected to western blot with primary antibodies including CHAC2, p-ERK and GAPDH. ( e ) The effect of ubiquitin on CHAC2 expression. AGS-CHAC2 and SW620-CHAC2 cells were incubated with indicated rhHA-ubiquitin for 12 h, and cell lysates were subjected to western blot with primary antibodies including CHAC2, HA tag andGAPDH. ( f ) 12 maximal upregulated genes checked by the Human Ubiquitination Pathway RT 2 Profiler PCR Array were shown in the bar graph. Among these genes, RNF148 expression significantly increased nearly 1863 times in SW620 as compared to SW48. ( g ) The expression of RNF148 in gastric and colorectal cancer cell lines was determined by RT-PCR. ( h ) Evaluation of RNF148 expression after treatment of 5-FU or BFA. 5-FU, 5-fluorouracil; BFA, Brefeldin A; CHX, Cycloheximide; Leu, leupeptin; CQ, Chloroquine; Ubi, rhHA-ubiquitin

    Techniques Used: Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot, Stable Transfection, Transfection, Incubation, Polymerase Chain Reaction

    5) Product Images from "Delayed triggering of oestrogen induced apoptosis that contrasts with rapid paclitaxel-induced breast cancer cell death"

    Article Title: Delayed triggering of oestrogen induced apoptosis that contrasts with rapid paclitaxel-induced breast cancer cell death

    Journal: British Journal of Cancer

    doi: 10.1038/bjc.2014.50

    Differential apoptotic effects of E 2 and paclitaxel. MCF7:5C cells were treated with control or ( A ) paclitaxel (1 μ M ) for 12 and 24 h or ( B ) E 2 (1 n M ) for 72 h, and then stained with annexin V-FITC and PI and analysed by flow cytometry. Viable cells (left lower quadrant) are annexin V-FITC− and PI−, early apoptotic cells (right lower quadrant) are annexin V-FITC+ and PI−, dead cells (left upper quadrant) are PI+ and late apoptotic cells (right upper quadrant) are annexin V-FITC+ and PI+. Increased staining for apoptosis is observed maximally in the right upper quadrant.
    Figure Legend Snippet: Differential apoptotic effects of E 2 and paclitaxel. MCF7:5C cells were treated with control or ( A ) paclitaxel (1 μ M ) for 12 and 24 h or ( B ) E 2 (1 n M ) for 72 h, and then stained with annexin V-FITC and PI and analysed by flow cytometry. Viable cells (left lower quadrant) are annexin V-FITC− and PI−, early apoptotic cells (right lower quadrant) are annexin V-FITC+ and PI−, dead cells (left upper quadrant) are PI+ and late apoptotic cells (right upper quadrant) are annexin V-FITC+ and PI+. Increased staining for apoptosis is observed maximally in the right upper quadrant.

    Techniques Used: Staining, Flow Cytometry, Cytometry

    Deciphering the trigger point for E 2 -induced apoptosis. Cells were treated with vehicle (Veh) or E 2 (1 n M ) alone, and 1 μ M 4OHT was added and used to block and reverse E 2 action at 6, 12, 24, 36, 48, 60, 72, 84 and 96 h. The cells were harvested after 7 days of treatment. The extent of apoptosis was determined by measuring the DNA content of the remaining cells in each well. The experiment was done in triplicates, and the data represent the mean of three independent experiments with 95% confidence intervals. The trigger point for E 2 -mediated apoptosis was elucidated at the time when the apoptotic effects of E 2 could not be blocked by 4OHT.
    Figure Legend Snippet: Deciphering the trigger point for E 2 -induced apoptosis. Cells were treated with vehicle (Veh) or E 2 (1 n M ) alone, and 1 μ M 4OHT was added and used to block and reverse E 2 action at 6, 12, 24, 36, 48, 60, 72, 84 and 96 h. The cells were harvested after 7 days of treatment. The extent of apoptosis was determined by measuring the DNA content of the remaining cells in each well. The experiment was done in triplicates, and the data represent the mean of three independent experiments with 95% confidence intervals. The trigger point for E 2 -mediated apoptosis was elucidated at the time when the apoptotic effects of E 2 could not be blocked by 4OHT.

    Techniques Used: Blocking Assay

    E 2 activates both mitochondrial and extrinsic pathway of apoptosis, whereas paclitaxel activates only the extrinsic pathway. MCF7:5C cells were treated with vehicle (Veh), 1 n M E2, 1 μ M 4OHT or combination treatment of E 2 and 4OHT for 24, 36 and 48 h. Total RNA was reverse transcribed and assessed for ( A ) BIM and ( B ) TNF gene expression. Induction of ( C ) BIM and ( D ) TNF mRNA was determined in MCF7:5C cells treated with either Veh or 1 μ M paclitaxel for 12 and 24 h using RT–PCR. PCR data values are presented as fold difference versus Veh-treated cells±s.e.m. (** P
    Figure Legend Snippet: E 2 activates both mitochondrial and extrinsic pathway of apoptosis, whereas paclitaxel activates only the extrinsic pathway. MCF7:5C cells were treated with vehicle (Veh), 1 n M E2, 1 μ M 4OHT or combination treatment of E 2 and 4OHT for 24, 36 and 48 h. Total RNA was reverse transcribed and assessed for ( A ) BIM and ( B ) TNF gene expression. Induction of ( C ) BIM and ( D ) TNF mRNA was determined in MCF7:5C cells treated with either Veh or 1 μ M paclitaxel for 12 and 24 h using RT–PCR. PCR data values are presented as fold difference versus Veh-treated cells±s.e.m. (** P

    Techniques Used: Expressing, Reverse Transcription Polymerase Chain Reaction, Polymerase Chain Reaction

    6) Product Images from "Systematic Mapping of WNT-FZD Protein Interactions Reveals Functional Selectivity by Distinct WNT-FZD Pairs *"

    Article Title: Systematic Mapping of WNT-FZD Protein Interactions Reveals Functional Selectivity by Distinct WNT-FZD Pairs *

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M114.612648

    32D myeloid progenitor cell line expresses little or no endogenous FZDs but does express LRP5/6 co-receptors. A , the bar graph presents the FZD expression profile (FZD 1–10 ) of 32D cells ( hatched gray bars ) and primary microglia cells ( white bars ) determined by qPCR ( n ≥ 3). Error bars represent S.E. GAPDH was used as a housekeeping gene for normalization. By comparing FZD expression in primary microglia cells (previously published by Halleskog et al. )) and 32D cells, it becomes apparent that FZD levels in 32D cells are very low. B , WNT co-receptor profile in 32D cells determined by RT-PCR. LRP5 and LRP6 (but neither RYK nor ROR1/2) are expressed in 32D cells. The negative control consisted of 32D cell cDNA prepared without reverse transcriptase; mouse tail genomic DNA or cDNA from GL261 cells (RYK) was used as a positive control. C , shown are comparative PCR expression profiles of 32D and L929 cells, including WNTs, FZDs, LRPs, DKKs, and SFRPs. The table summarizes the average C t values of two biological replicates. The following genes were not included on the PCR array: Wnt9b , Wnt10b , Fzd9 , Fzd10 , Ryk , Ror1 , and Ror2. D , immunoblotting for HA-tagged FZDs in 32D cells expressing FZD 2 , FZD 4 , or FZD 5 . β-Actin was used as a loading control. The molecular masses of FZDs were determined with Bio-Rad software.
    Figure Legend Snippet: 32D myeloid progenitor cell line expresses little or no endogenous FZDs but does express LRP5/6 co-receptors. A , the bar graph presents the FZD expression profile (FZD 1–10 ) of 32D cells ( hatched gray bars ) and primary microglia cells ( white bars ) determined by qPCR ( n ≥ 3). Error bars represent S.E. GAPDH was used as a housekeeping gene for normalization. By comparing FZD expression in primary microglia cells (previously published by Halleskog et al. )) and 32D cells, it becomes apparent that FZD levels in 32D cells are very low. B , WNT co-receptor profile in 32D cells determined by RT-PCR. LRP5 and LRP6 (but neither RYK nor ROR1/2) are expressed in 32D cells. The negative control consisted of 32D cell cDNA prepared without reverse transcriptase; mouse tail genomic DNA or cDNA from GL261 cells (RYK) was used as a positive control. C , shown are comparative PCR expression profiles of 32D and L929 cells, including WNTs, FZDs, LRPs, DKKs, and SFRPs. The table summarizes the average C t values of two biological replicates. The following genes were not included on the PCR array: Wnt9b , Wnt10b , Fzd9 , Fzd10 , Ryk , Ror1 , and Ror2. D , immunoblotting for HA-tagged FZDs in 32D cells expressing FZD 2 , FZD 4 , or FZD 5 . β-Actin was used as a loading control. The molecular masses of FZDs were determined with Bio-Rad software.

    Techniques Used: Expressing, Real-time Polymerase Chain Reaction, Reverse Transcription Polymerase Chain Reaction, Negative Control, Positive Control, Polymerase Chain Reaction, Software

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    Real-time Polymerase Chain Reaction:

    Article Title: Regulation of the expression of tumor necrosis factor-related genes by abnormal histone H3K27 acetylation: Implications for neural tube defects
    Article Snippet: RNA extraction and reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis Total RNA was isolated from tissue samples using TRIzol (cat. no. 15596026; Invitrogen; Thermo Fisher Scientific, Inc., Waltham, MA, USA) and analyzed on an Applied Biosystems 7500 Real-Time PCR system (Applied Biosystems; Thermo Fisher Scientific, Inc.) using a first strand cDNA synthesis kit (K1612; TransGen Biotech Co., Ltd., Beijing, China). .. The data were analyzed using Qiagen RT2 Profiler PCR Array Data Analysis version 3.5 (Qiagen GmbH; product. no. 330231; cat. no. PAMM-012Z).

    Article Title: The heme and radical scavenger α1-microglobulin (A1M) confers early protection of the immature brain following preterm intraventricular hemorrhage
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    Article Title: Mitotane induces mitochondrial membrane depolarization and apoptosis in thyroid cancer cells
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    RNA Extraction:

    Article Title: Regulation of the expression of tumor necrosis factor-related genes by abnormal histone H3K27 acetylation: Implications for neural tube defects
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    Article Title: Mitotane induces mitochondrial membrane depolarization and apoptosis in thyroid cancer cells
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    Article Title: Antiretroviral therapy, Interferon Sensitivity, and Virologic Set Point in HIV-Hepatitis C Virus Co-infected Patients
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    Polymerase Chain Reaction:

    Article Title: Regulation of the expression of tumor necrosis factor-related genes by abnormal histone H3K27 acetylation: Implications for neural tube defects
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    Article Title: The heme and radical scavenger α1-microglobulin (A1M) confers early protection of the immature brain following preterm intraventricular hemorrhage
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    Article Title: Targeting S1PR1/STAT3 loop abrogates desmoplasia and chemosensitizes pancreatic cancer to gemcitabine
    Article Snippet: .. The cDNA was then mixed with SYBR green PCR master mix (ABI, #4367659) and 25 µL per well was added in RT² Profiler PCR Array (Qiagen, #PAHS-181Z) with , 96-well plate format. .. The data were then exported and uploaded into the RT2 profiler PCR array web portal ( http://pcrdataanalysis.sabiosciences.com/pcr/arrayanalysis.php ) for analysis.

    Article Title: Mitotane induces mitochondrial membrane depolarization and apoptosis in thyroid cancer cells
    Article Snippet: Paragraph title: RNA extraction and reverse transcription-quantitative (RT-q) PCR ... A total of 1 μ g RNA was reverse transcribed into cDNA with the miScript II RT kit (Qiagen, Inc.) as indicated, at 37°C for 1 h and 95°C for 5 min for termination of the reaction. qPCR screening of cancer-associated genes was performed using a pre-fabricated RT-PCR Human Cancer PathwayFinder Array (PAHS-033ZA; cat. no. 330231) and RT2 SYBR® Green qPCR Mastermix (both Qiagen, Inc.).

    Article Title: Antiretroviral therapy, Interferon Sensitivity, and Virologic Set Point in HIV-Hepatitis C Virus Co-infected Patients
    Article Snippet: .. Expression of 89 ISGs was measured in the liver using the RT² Profiler PCR Array (Catalog #: PAHS-016Z, Qiagen, USA). ..

    Article Title: Deregulation of ATG9A by impaired AR signaling induces autophagy in prostate stromal fibroblasts and promotes BPH progression
    Article Snippet: .. RT² Profiler PCR Array (Qiagen, Hilden, Germany) was used for assessing the expression levels of ATG genes in prostate stromal fibroblasts after treatment with different DHT concentrations. .. Relative mRNA expression was calculated by 2−ΔΔCt method.

    Article Title: Antiretroviral therapy, Interferon Sensitivity, and Virologic Set Point in HIV-Hepatitis C Virus Co-infected Patients
    Article Snippet: .. After extraction, mRNA for interferon stimulated genes (ISGs) was quantified according to the RT² Profiler PCR Array (Qiagen, USA) manufacturer protocol with addition of DNAse treatment to remove genomic DNA. .. Expression of 89 ISGs was measured in the liver using the RT² Profiler PCR Array (Catalog #: PAHS-016Z, Qiagen, USA).

    Article Title: The nuclear phosphatase SCP4 regulates FoxO transcription factors during muscle wasting in chronic kidney disease
    Article Snippet: .. RT² Profiler™ PCR Array (Myogenesis & Myopathy, PAMM-099Z) was performed following the manufactory’s instruction (Qiagen Sciences). ..

    Article Title: Acute Noise Exposure Is Associated With Intrinsic Apoptosis in Murine Central Auditory Pathway
    Article Snippet: .. The cDNA templates were mixed with ready-to-use SYBR Green Mastermix (RT2 SYBR Green qPSR Mastermix, Qiagen, Cat. No. 330502), and loaded onto the 96-well RT2 -PCR array (PAMM-212ZF-6- RT2 Profiler™ PCR Array Mouse Cell Death Pathway Finder, Qiagen, Cat. No. 330231; see Table for detailed list of genes). ..

    Article Title: Mechanisms of Diabetes-Induced Endothelial Cell Senescence: Role of Arginase 1
    Article Snippet: .. Quantitative PCR was performed following the manufacturer’s protocol using Mouse Cellular Senescence RT2 Profiler PCR Array (Qiagen Inc., Germantown, MD, USA; Cat. # 330231 PAMM-050ZA) containing primers of 84 testing genes that are involved in the cellular senescence process. β-actin was used as the internal control gene. ..

    Isolation:

    Article Title: Regulation of the expression of tumor necrosis factor-related genes by abnormal histone H3K27 acetylation: Implications for neural tube defects
    Article Snippet: RNA extraction and reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis Total RNA was isolated from tissue samples using TRIzol (cat. no. 15596026; Invitrogen; Thermo Fisher Scientific, Inc., Waltham, MA, USA) and analyzed on an Applied Biosystems 7500 Real-Time PCR system (Applied Biosystems; Thermo Fisher Scientific, Inc.) using a first strand cDNA synthesis kit (K1612; TransGen Biotech Co., Ltd., Beijing, China). .. The data were analyzed using Qiagen RT2 Profiler PCR Array Data Analysis version 3.5 (Qiagen GmbH; product. no. 330231; cat. no. PAMM-012Z).

    Article Title: The heme and radical scavenger α1-microglobulin (A1M) confers early protection of the immature brain following preterm intraventricular hemorrhage
    Article Snippet: Paragraph title: RNA isolation and real-time PCR ... RT2 PCR Profiler Array real-time PCR (QIAGEN custom made, Cat no. CAPN11841 and Wound healing, Cat no. 330231 PANZ-121ZA) were used to quantify the mRNA expression of toll-like receptor (TLR)-4, monocyte chemoatractant protein (MCP)-1, interleukin (IL)-1β, IL-6, IL-8, IL1 receptor (R)1, tumor necrosis factor (TNF) α, matrix metalloprotease (MMP) 9, and heme oxygenase (HO)-1.

    Article Title: Targeting S1PR1/STAT3 loop abrogates desmoplasia and chemosensitizes pancreatic cancer to gemcitabine
    Article Snippet: RT2 profiler PCR array RNA was isolated by RNeasy Lipid Tissue mini kit (Qiagen, #74804) with a DNase treatment step included. .. The cDNA was then mixed with SYBR green PCR master mix (ABI, #4367659) and 25 µL per well was added in RT² Profiler PCR Array (Qiagen, #PAHS-181Z) with , 96-well plate format.

    Quantitative RT-PCR:

    Article Title: Regulation of the expression of tumor necrosis factor-related genes by abnormal histone H3K27 acetylation: Implications for neural tube defects
    Article Snippet: Paragraph title: RNA extraction and reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis ... The data were analyzed using Qiagen RT2 Profiler PCR Array Data Analysis version 3.5 (Qiagen GmbH; product. no. 330231; cat. no. PAMM-012Z).

    Article Title: Deregulation of ATG9A by impaired AR signaling induces autophagy in prostate stromal fibroblasts and promotes BPH progression
    Article Snippet: Paragraph title: qRT-PCR and PCR array ... RT² Profiler PCR Array (Qiagen, Hilden, Germany) was used for assessing the expression levels of ATG genes in prostate stromal fibroblasts after treatment with different DHT concentrations.

    SYBR Green Assay:

    Article Title: Targeting S1PR1/STAT3 loop abrogates desmoplasia and chemosensitizes pancreatic cancer to gemcitabine
    Article Snippet: .. The cDNA was then mixed with SYBR green PCR master mix (ABI, #4367659) and 25 µL per well was added in RT² Profiler PCR Array (Qiagen, #PAHS-181Z) with , 96-well plate format. .. The data were then exported and uploaded into the RT2 profiler PCR array web portal ( http://pcrdataanalysis.sabiosciences.com/pcr/arrayanalysis.php ) for analysis.

    Article Title: Mitotane induces mitochondrial membrane depolarization and apoptosis in thyroid cancer cells
    Article Snippet: .. A total of 1 μ g RNA was reverse transcribed into cDNA with the miScript II RT kit (Qiagen, Inc.) as indicated, at 37°C for 1 h and 95°C for 5 min for termination of the reaction. qPCR screening of cancer-associated genes was performed using a pre-fabricated RT-PCR Human Cancer PathwayFinder Array (PAHS-033ZA; cat. no. 330231) and RT2 SYBR® Green qPCR Mastermix (both Qiagen, Inc.). .. The arrays were run on the QuantStudio™ 6 Flex Real-Time PCR System (Thermo Fisher Scientific, Inc.).

    Article Title: Acute Noise Exposure Is Associated With Intrinsic Apoptosis in Murine Central Auditory Pathway
    Article Snippet: .. The cDNA templates were mixed with ready-to-use SYBR Green Mastermix (RT2 SYBR Green qPSR Mastermix, Qiagen, Cat. No. 330502), and loaded onto the 96-well RT2 -PCR array (PAMM-212ZF-6- RT2 Profiler™ PCR Array Mouse Cell Death Pathway Finder, Qiagen, Cat. No. 330231; see Table for detailed list of genes). ..

    Microarray:

    Article Title: Antiretroviral therapy, Interferon Sensitivity, and Virologic Set Point in HIV-Hepatitis C Virus Co-infected Patients
    Article Snippet: A portion of liver tissue pre- and post-ART for each participant was homogenized and processed for RNA extraction using the RNeasy Microarray Tissue Mini Kit (Qiagen, USA). .. Expression of 89 ISGs was measured in the liver using the RT² Profiler PCR Array (Catalog #: PAHS-016Z, Qiagen, USA).

    Spectrophotometry:

    Article Title: Targeting S1PR1/STAT3 loop abrogates desmoplasia and chemosensitizes pancreatic cancer to gemcitabine
    Article Snippet: RNA was quantified using Nanodrop spectrophotometer 2000. .. The cDNA was then mixed with SYBR green PCR master mix (ABI, #4367659) and 25 µL per well was added in RT² Profiler PCR Array (Qiagen, #PAHS-181Z) with , 96-well plate format.

    Article Title: Mitotane induces mitochondrial membrane depolarization and apoptosis in thyroid cancer cells
    Article Snippet: The quality and quantity of total RNA was assessed with a NanoDrop 1000 spectrophotometer (Thermo Fisher Scientific, Inc.). .. A total of 1 μ g RNA was reverse transcribed into cDNA with the miScript II RT kit (Qiagen, Inc.) as indicated, at 37°C for 1 h and 95°C for 5 min for termination of the reaction. qPCR screening of cancer-associated genes was performed using a pre-fabricated RT-PCR Human Cancer PathwayFinder Array (PAHS-033ZA; cat. no. 330231) and RT2 SYBR® Green qPCR Mastermix (both Qiagen, Inc.).

    Expressing:

    Article Title: Regulation of the expression of tumor necrosis factor-related genes by abnormal histone H3K27 acetylation: Implications for neural tube defects
    Article Snippet: The Mouse Apoptosis PCR Array was used to profile the expression of 84 key genes involved in programmed cell death. .. The data were analyzed using Qiagen RT2 Profiler PCR Array Data Analysis version 3.5 (Qiagen GmbH; product. no. 330231; cat. no. PAMM-012Z).

    Article Title: The heme and radical scavenger α1-microglobulin (A1M) confers early protection of the immature brain following preterm intraventricular hemorrhage
    Article Snippet: .. RT2 PCR Profiler Array real-time PCR (QIAGEN custom made, Cat no. CAPN11841 and Wound healing, Cat no. 330231 PANZ-121ZA) were used to quantify the mRNA expression of toll-like receptor (TLR)-4, monocyte chemoatractant protein (MCP)-1, interleukin (IL)-1β, IL-6, IL-8, IL1 receptor (R)1, tumor necrosis factor (TNF) α, matrix metalloprotease (MMP) 9, and heme oxygenase (HO)-1. .. Data were normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH, custom made by QIAGEN, Cat no. CAPN11841).

    Article Title: Antiretroviral therapy, Interferon Sensitivity, and Virologic Set Point in HIV-Hepatitis C Virus Co-infected Patients
    Article Snippet: .. Expression of 89 ISGs was measured in the liver using the RT² Profiler PCR Array (Catalog #: PAHS-016Z, Qiagen, USA). ..

    Article Title: Deregulation of ATG9A by impaired AR signaling induces autophagy in prostate stromal fibroblasts and promotes BPH progression
    Article Snippet: .. RT² Profiler PCR Array (Qiagen, Hilden, Germany) was used for assessing the expression levels of ATG genes in prostate stromal fibroblasts after treatment with different DHT concentrations. .. Relative mRNA expression was calculated by 2−ΔΔCt method.

    Article Title: Antiretroviral therapy, Interferon Sensitivity, and Virologic Set Point in HIV-Hepatitis C Virus Co-infected Patients
    Article Snippet: After extraction, mRNA for interferon stimulated genes (ISGs) was quantified according to the RT² Profiler PCR Array (Qiagen, USA) manufacturer protocol with addition of DNAse treatment to remove genomic DNA. .. Expression of 89 ISGs was measured in the liver using the RT² Profiler PCR Array (Catalog #: PAHS-016Z, Qiagen, USA).

    Article Title: Acute Noise Exposure Is Associated With Intrinsic Apoptosis in Murine Central Auditory Pathway
    Article Snippet: RT2 -PCR array experiments The noise-induced expression of 84 cell death related genes have been profiled using a commercial PCR array in the first part of experiments. .. The cDNA templates were mixed with ready-to-use SYBR Green Mastermix (RT2 SYBR Green qPSR Mastermix, Qiagen, Cat. No. 330502), and loaded onto the 96-well RT2 -PCR array (PAMM-212ZF-6- RT2 Profiler™ PCR Array Mouse Cell Death Pathway Finder, Qiagen, Cat. No. 330231; see Table for detailed list of genes).

    Article Title: Mechanisms of Diabetes-Induced Endothelial Cell Senescence: Role of Arginase 1
    Article Snippet: PCR Analyses A PCR array was used to examine senescence-related mRNA expression. .. Quantitative PCR was performed following the manufacturer’s protocol using Mouse Cellular Senescence RT2 Profiler PCR Array (Qiagen Inc., Germantown, MD, USA; Cat. # 330231 PAMM-050ZA) containing primers of 84 testing genes that are involved in the cellular senescence process. β-actin was used as the internal control gene.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Mitotane induces mitochondrial membrane depolarization and apoptosis in thyroid cancer cells
    Article Snippet: .. A total of 1 μ g RNA was reverse transcribed into cDNA with the miScript II RT kit (Qiagen, Inc.) as indicated, at 37°C for 1 h and 95°C for 5 min for termination of the reaction. qPCR screening of cancer-associated genes was performed using a pre-fabricated RT-PCR Human Cancer PathwayFinder Array (PAHS-033ZA; cat. no. 330231) and RT2 SYBR® Green qPCR Mastermix (both Qiagen, Inc.). .. The arrays were run on the QuantStudio™ 6 Flex Real-Time PCR System (Thermo Fisher Scientific, Inc.).

    Software:

    Article Title: Mitotane induces mitochondrial membrane depolarization and apoptosis in thyroid cancer cells
    Article Snippet: A total of 1 μ g RNA was reverse transcribed into cDNA with the miScript II RT kit (Qiagen, Inc.) as indicated, at 37°C for 1 h and 95°C for 5 min for termination of the reaction. qPCR screening of cancer-associated genes was performed using a pre-fabricated RT-PCR Human Cancer PathwayFinder Array (PAHS-033ZA; cat. no. 330231) and RT2 SYBR® Green qPCR Mastermix (both Qiagen, Inc.). .. Cycle thresholds were determined for each gene using the QuantStudio 6 Flex software.

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    Qiagen rt² profiler pcr array
    Rt² Profiler Pcr Array, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 81 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rt² profiler pcr array/product/Qiagen
    Average 90 stars, based on 81 article reviews
    Price from $9.99 to $1999.99
    rt² profiler pcr array - by Bioz Stars, 2020-01
    90/100 stars
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    For recreating Screening Suite conditions Kit contents 200 ml 1M Cadmium chloride anhydrous
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