3195l t4 dna ligase reaction buffer new england biolabs cat  (New England Biolabs)


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  • 99
    Name:
    EcoRV HF
    Description:
    EcoRV HF 20 000 units
    Catalog Number:
    r3195l
    Price:
    249
    Size:
    20 000 units
    Category:
    Restriction Enzymes
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    Structured Review

    New England Biolabs 3195l t4 dna ligase reaction buffer new england biolabs cat
    EcoRV HF
    EcoRV HF 20 000 units
    https://www.bioz.com/result/3195l t4 dna ligase reaction buffer new england biolabs cat/product/New England Biolabs
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    3195l t4 dna ligase reaction buffer new england biolabs cat - by Bioz Stars, 2020-08
    99/100 stars

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    Related Articles

    Luciferase:

    Article Title: Ex Vivo Expansion of Murine MSC Impairs Transcription Factor-Induced Differentiation into Pancreatic β-Cells
    Article Snippet: .. The luciferase reporter gene Luc2 (Photinus pyralis ), encoded within the vector pGL4.20 (Luc2 /Puro) (Promega® , Ipswich, USA), was digested with the restriction enzymes, EcoRV-HF® and BamHI-HF® (New England Biolabs® , San Diego, USA), and ligated into the mammalian dual expression plasmid pVITRO2-hygro® -mcs (InvivoGen® , San Diego, USA) to generate the mammalian bioluminescence plasmid pVITRO2-Luc2 (Supplementary ). .. Nucleofection Early passage MSCs (1 × 106 cells) were nucleofected with 5 μ g pVITRO2-Luc2 and 2 μ g pmax-GFP® , according to the manufacturer's instructions (Lonza™, Basel, Switzerland), using the Nucleofector™ 2b device (Lonza™).

    In Vitro:

    Article Title: Structural Features of a 3′ Splice Site in Influenza A
    Article Snippet: .. The plasmid was linearized with EcoRV-HF restriction endonuclease (New England BioLabs) at 37 °C prior to in vitro transcription. ..

    Construct:

    Article Title: Translocation of Dense Granule Effectors across the Parasitophorous Vacuole Membrane in Toxoplasma-Infected Cells Requires the Activity of ROP17, a Rhoptry Protein Kinase
    Article Snippet: .. To construct the pGRA-ROP17-3xHA plasmid, pGRA1plus -HPT-3xHA plasmid ( ) was first digested using EcoRV-HF and NcoI (New England Biolabs) for 4 h at 37°C to remove the GRA1 promoter. .. Product was incubated with Antarctic phosphatase (New England Biolabs) and gel extracted.

    Concentration Assay:

    Article Title: Synthetic Transient Crosslinks Program the Mechanics of Soft, Biopolymer-Based Materials
    Article Snippet: .. Prior to rheology measurements, 1624 ng sPX (final concentration of 0.24 × 10−6 m in 175 μL final sample volume; R = 0.01) were incubated with 5 μL of EcoRV-HF (20 000 units per milliliter, New England Biolabs Inc., USA) and 1.75 μL of 10x CutSmart buffer in a final volume of 20 μL for 1 h at 37 °C. .. Corresponding PAGE analysis showed a digestion rate of at least 80% after 1 h of incubation (Figure S2, ).

    Incubation:

    Article Title: Synthetic Transient Crosslinks Program the Mechanics of Soft, Biopolymer-Based Materials
    Article Snippet: .. Prior to rheology measurements, 1624 ng sPX (final concentration of 0.24 × 10−6 m in 175 μL final sample volume; R = 0.01) were incubated with 5 μL of EcoRV-HF (20 000 units per milliliter, New England Biolabs Inc., USA) and 1.75 μL of 10x CutSmart buffer in a final volume of 20 μL for 1 h at 37 °C. .. Corresponding PAGE analysis showed a digestion rate of at least 80% after 1 h of incubation (Figure S2, ).

    Expressing:

    Article Title: Ex Vivo Expansion of Murine MSC Impairs Transcription Factor-Induced Differentiation into Pancreatic β-Cells
    Article Snippet: .. The luciferase reporter gene Luc2 (Photinus pyralis ), encoded within the vector pGL4.20 (Luc2 /Puro) (Promega® , Ipswich, USA), was digested with the restriction enzymes, EcoRV-HF® and BamHI-HF® (New England Biolabs® , San Diego, USA), and ligated into the mammalian dual expression plasmid pVITRO2-hygro® -mcs (InvivoGen® , San Diego, USA) to generate the mammalian bioluminescence plasmid pVITRO2-Luc2 (Supplementary ). .. Nucleofection Early passage MSCs (1 × 106 cells) were nucleofected with 5 μ g pVITRO2-Luc2 and 2 μ g pmax-GFP® , according to the manufacturer's instructions (Lonza™, Basel, Switzerland), using the Nucleofector™ 2b device (Lonza™).

    Polymerase Chain Reaction:

    Article Title: The Development of a Viral Mediated CRISPR/Cas9 System with Doxycycline Dependent gRNA Expression for Inducible In vitro and In vivo Genome Editing
    Article Snippet: .. Three microliters of PCR product was digested with 5 units of EcoRV-HF (New England Biolabs) for 2 h in a standard 10 μl restriction enzyme reaction following the manufacturer's instructions. .. Each 3 μL of digested PCR product was electrophoresed on a 2.0% agarose, 0.5 X TBE gel to determine if genome editing had occurred.

    Plasmid Preparation:

    Article Title: Structural Features of a 3′ Splice Site in Influenza A
    Article Snippet: .. The plasmid was linearized with EcoRV-HF restriction endonuclease (New England BioLabs) at 37 °C prior to in vitro transcription. ..

    Article Title: A transcriptional circuit filters oscillating circadian hormonal inputs to regulate fat cell differentiation
    Article Snippet: .. The pENTR1a backbone vector was digested with EcoR Ι-HF and BamH Ι-HF (NEB), and assembled together with three DNA fragments coding for homology arm 1, Citrine or mKate2, and homology arm 2 using Gibson assembly. .. The homology arm fragments were PCR amplified from OP9 genomic DNA with primers introducing a 15–20 bp overhang used for Gibson assembly.

    Article Title: Ex Vivo Expansion of Murine MSC Impairs Transcription Factor-Induced Differentiation into Pancreatic β-Cells
    Article Snippet: .. The luciferase reporter gene Luc2 (Photinus pyralis ), encoded within the vector pGL4.20 (Luc2 /Puro) (Promega® , Ipswich, USA), was digested with the restriction enzymes, EcoRV-HF® and BamHI-HF® (New England Biolabs® , San Diego, USA), and ligated into the mammalian dual expression plasmid pVITRO2-hygro® -mcs (InvivoGen® , San Diego, USA) to generate the mammalian bioluminescence plasmid pVITRO2-Luc2 (Supplementary ). .. Nucleofection Early passage MSCs (1 × 106 cells) were nucleofected with 5 μ g pVITRO2-Luc2 and 2 μ g pmax-GFP® , according to the manufacturer's instructions (Lonza™, Basel, Switzerland), using the Nucleofector™ 2b device (Lonza™).

    Article Title: Translocation of Dense Granule Effectors across the Parasitophorous Vacuole Membrane in Toxoplasma-Infected Cells Requires the Activity of ROP17, a Rhoptry Protein Kinase
    Article Snippet: .. To construct the pGRA-ROP17-3xHA plasmid, pGRA1plus -HPT-3xHA plasmid ( ) was first digested using EcoRV-HF and NcoI (New England Biolabs) for 4 h at 37°C to remove the GRA1 promoter. .. Product was incubated with Antarctic phosphatase (New England Biolabs) and gel extracted.

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    New England Biolabs 3195l t4 dna ligase reaction buffer new england biolabs cat
    3195l T4 Dna Ligase Reaction Buffer New England Biolabs Cat, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/3195l t4 dna ligase reaction buffer new england biolabs cat/product/New England Biolabs
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    3195l t4 dna ligase reaction buffer new england biolabs cat - by Bioz Stars, 2020-08
    99/100 stars
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