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(a) The flow chart of <t>NPs-OVA</t> immunized animals. (b) The serum levels <t>of</t> <t>Anti-OVA</t> IgG as measured by <t>ELISA.</t> (c) The serum levels of IL-2 measured by ELISA. (d, e) The ELISpot assay results for the secretion of IL-4 and IFN-γ by splenic cells. The P-value of less than 0.05 was considered statistically significant (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001).
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(a) The flow chart of <t>NPs-OVA</t> immunized animals. (b) The serum levels <t>of</t> <t>Anti-OVA</t> IgG as measured by <t>ELISA.</t> (c) The serum levels of IL-2 measured by ELISA. (d, e) The ELISpot assay results for the secretion of IL-4 and IFN-γ by splenic cells. The P-value of less than 0.05 was considered statistically significant (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001).
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(a) The flow chart of <t>NPs-OVA</t> immunized animals. (b) The serum levels <t>of</t> <t>Anti-OVA</t> IgG as measured by <t>ELISA.</t> (c) The serum levels of IL-2 measured by ELISA. (d, e) The ELISpot assay results for the secretion of IL-4 and IFN-γ by splenic cells. The P-value of less than 0.05 was considered statistically significant (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001).
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(a) The flow chart of <t>NPs-OVA</t> immunized animals. (b) The serum levels <t>of</t> <t>Anti-OVA</t> IgG as measured by <t>ELISA.</t> (c) The serum levels of IL-2 measured by ELISA. (d, e) The ELISpot assay results for the secretion of IL-4 and IFN-γ by splenic cells. The P-value of less than 0.05 was considered statistically significant (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001).
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(a) The flow chart of <t>NPs-OVA</t> immunized animals. (b) The serum levels <t>of</t> <t>Anti-OVA</t> IgG as measured by <t>ELISA.</t> (c) The serum levels of IL-2 measured by ELISA. (d, e) The ELISpot assay results for the secretion of IL-4 and IFN-γ by splenic cells. The P-value of less than 0.05 was considered statistically significant (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001).
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(a) The flow chart of NPs-OVA immunized animals. (b) The serum levels of Anti-OVA IgG as measured by ELISA. (c) The serum levels of IL-2 measured by ELISA. (d, e) The ELISpot assay results for the secretion of IL-4 and IFN-γ by splenic cells. The P-value of less than 0.05 was considered statistically significant (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001).

Journal: bioRxiv

Article Title: Tunable Rigid Spikes on Virus-Like Porous Silica Enable Mechanistically Controlled Nanovaccine Platforms

doi: 10.64898/2026.04.26.720861

Figure Lengend Snippet: (a) The flow chart of NPs-OVA immunized animals. (b) The serum levels of Anti-OVA IgG as measured by ELISA. (c) The serum levels of IL-2 measured by ELISA. (d, e) The ELISpot assay results for the secretion of IL-4 and IFN-γ by splenic cells. The P-value of less than 0.05 was considered statistically significant (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001).

Article Snippet: Mouse IL-4 ELISA Kit (NBS EMC002.96), Mouse IFN-γ ELISA Kit (NBS EMC101g.96), Mouse IFN-γ ELISpotSASIC (HRP) (Mabtech 3321-2H), Mouse IL-4 ELISpotSASIC (HRP) (Mabtech 3311-2H), Tetramethylbenzidine (TMB) substrate for ELISpot (Mabtech 3651-10), OVA IgG ELISA Kit (Chondrex 3011), OVA IgM ELISA Kit (Chondrex 3017), and Low Endotoxin Ovalbumin from Chick Egg White (Chondrex 3022).

Techniques: Enzyme-linked Immunosorbent Assay, Enzyme-linked Immunospot

Results of subcutaneous injection of NPs-rEsxB in mice and subjected to lethal challenge with S. aureus. (a) The experimental flow chart for the lethal challenge. (b) The total serum rEsxB-specific IgG titer assay in NPs-rEsxB-immunized mice. (c, d) ELISA quantification of IL-4 and IFN-γ in mice. (e, f) The ELISpot assay results for the secretion of IL-4 and IFN-γ by splenic cells. (g, h) The survival rate of 1×LD100 S. aureus challenge experiment. (i, j, k) The survival rate of 2×LD100 S. aureus challenge experiment. All data are expressed as mean ± standard deviation (S.D.). The P-value of less than 0.05 was considered statistically significant (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001).

Journal: bioRxiv

Article Title: Tunable Rigid Spikes on Virus-Like Porous Silica Enable Mechanistically Controlled Nanovaccine Platforms

doi: 10.64898/2026.04.26.720861

Figure Lengend Snippet: Results of subcutaneous injection of NPs-rEsxB in mice and subjected to lethal challenge with S. aureus. (a) The experimental flow chart for the lethal challenge. (b) The total serum rEsxB-specific IgG titer assay in NPs-rEsxB-immunized mice. (c, d) ELISA quantification of IL-4 and IFN-γ in mice. (e, f) The ELISpot assay results for the secretion of IL-4 and IFN-γ by splenic cells. (g, h) The survival rate of 1×LD100 S. aureus challenge experiment. (i, j, k) The survival rate of 2×LD100 S. aureus challenge experiment. All data are expressed as mean ± standard deviation (S.D.). The P-value of less than 0.05 was considered statistically significant (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001).

Article Snippet: Mouse IL-4 ELISA Kit (NBS EMC002.96), Mouse IFN-γ ELISA Kit (NBS EMC101g.96), Mouse IFN-γ ELISpotSASIC (HRP) (Mabtech 3321-2H), Mouse IL-4 ELISpotSASIC (HRP) (Mabtech 3311-2H), Tetramethylbenzidine (TMB) substrate for ELISpot (Mabtech 3651-10), OVA IgG ELISA Kit (Chondrex 3011), OVA IgM ELISA Kit (Chondrex 3017), and Low Endotoxin Ovalbumin from Chick Egg White (Chondrex 3022).

Techniques: Injection, Titer Assay, Enzyme-linked Immunosorbent Assay, Enzyme-linked Immunospot, Standard Deviation