3 dithio bis  (Millipore)


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  • 99
    Name:
    3 3 Dithiobis propionitrile
    Description:

    Catalog Number:
    484717
    Price:
    None
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    Structured Review

    Millipore 3 dithio bis
    3 3 Dithiobis propionitrile

    https://www.bioz.com/result/3 dithio bis/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    3 dithio bis - by Bioz Stars, 2020-04
    99/100 stars

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    Related Articles

    Conjugation Assay:

    Article Title: Macrophages offer a paradigm switch for CNS delivery of therapeutic proteins
    Article Snippet: Bis-(sulfosuccinimidyl)suberate sodium salt (BS3), 3,3’-dithiobis-(sulfosuccinimidyl propionate) (DTSSP), 6-hydroxydopamine (6-OHDA), lipopolysaccharides (LPS), PEI (2K, branched, 50% aqueous solution), rhodamine isothiocyanate, and Triton™ X-100 were from Sigma-Aldrich (MO, USA). .. PEI (2K)–PEG (10K) was synthesized as described previously by conjugation of PEI and methoxypolyethylene glycol epoxy [ ].

    Article Title: Microfluidic Devices Integrating Microcavity Surface-Plasmon-Resonance Sensors: Glucose Oxidase Binding-Activity Detection
    Article Snippet: We used 3,3′-dithio-bis(sulfosuccinimidyl propionate) ( DTSSP, Calbiochem, EMD Chemicals, Inc.), a water-soluble, homo-, bi-functional, thiol-cleavable zero-length cross-linker that covalently binds to gold surfaces through its disulfide group and to proteins through its amine-reactive N -hydroxysulfosuccinimide ( sulfo-NHS ) ester. .. DTSSP hydrolyzes rapidly in aqueous solution and both hydrolysis and conjugation release NHS as a leaving group.

    Centrifugation:

    Article Title: Influence of KDEL on the Fate of Trimeric or Assembly-Defective Phaseolin
    Article Snippet: Protoplast (∼300,000, either unlabeled or radioactively labeled with ProMix for 3 hr) were pelleted by addition of 3 volumes of ice-cold W5 medium (154 mM NaCl, 5 mM KCl, 125 mM CaCl2 ·2H2 O, 5 mM glucose) followed by centrifugation at 60 g for 10 min at 4°C and washed once again with W5, leaving 50 μL to cover the pellet. .. Eight microliters of anhydrous DMSO supplemented or not with 12.5 mM dithiobis(succinimidyl propionate) (DSP; Sigma) were added, and the sample was incubated 30 min on ice with occasional gentle mixing.

    Article Title: Generation and Characterization of Antibodies against Opioid Receptors from Zebrafish
    Article Snippet: Triton X-100 was added to the sonicated solution and centrifugation was performed to remove the debris. .. To generate the affinity columns to purify antibodies, recombinant proteins bound to glutathione beads were covalently crosslinked with dithiobis(succinimidyl propionate) reagent (Sigma-Aldrich, St. Louis, MO, USA) following the instructions of the manufacturer.

    UV-Vis Spectroscopy:

    Article Title: Seedless gold nanostars with seed-like advantages for biosensing applications
    Article Snippet: Materials and instrumentation Hydrochloroauric acid (HAuCl4 ), glucose oxidase (GOx), trisodium citrate, silver nitrate (AgNO3 ), ascorbic acid, sodium chloride (NaCl), polyvinylpyrrolidone (PVP) (molecular weight 10 000), hydrochloric acid (HCl), glucose, 3 3′-dithiobis (sulfosuccinimidyl propionate) (DTSSP), 2-(N-morpholino)ethanesulfonic acid (MES) at pH 6, (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) acid (HEPES) at pH 7.4, Tris-acetate-EDTA (TAE) at pH 8, bovine serum albumin (BSA), ethanolamine and phosphate buffered saline (PBS) at pH 7.4 were all purchased from Sigma-Aldrich, South Africa. .. UV–vis spectroscopy analysis was carried out by spectral scanning (300–900 nm) on a HT Synergy (BioTEK) microplate reader.

    Synthesized:

    Article Title: Macrophages offer a paradigm switch for CNS delivery of therapeutic proteins
    Article Snippet: Bis-(sulfosuccinimidyl)suberate sodium salt (BS3), 3,3’-dithiobis-(sulfosuccinimidyl propionate) (DTSSP), 6-hydroxydopamine (6-OHDA), lipopolysaccharides (LPS), PEI (2K, branched, 50% aqueous solution), rhodamine isothiocyanate, and Triton™ X-100 were from Sigma-Aldrich (MO, USA). .. PEI (2K)–PEG (10K) was synthesized as described previously by conjugation of PEI and methoxypolyethylene glycol epoxy [ ].

    Protease Inhibitor:

    Article Title: Syntrophin-dependent expression and localization of Aquaporin-4 water channel protein
    Article Snippet: Sprague–Dawley rat cerebellum (130 mg) was homogenized by Potter–Elvehjem at 4°C in 5 ml of 7.5 mM sodium phosphate (pH 7.0)/0.25 M sucrose/5 mM EDTA/5 mM EGTA, with Complete Protease Inhibitor Cocktail (Roche Diagnostics). .. After a 10-min 1,000 × g spin at 4°C, supernatants were incubated 30 min at 4°C with 150 μM dithiobis(succinimidyl propionate) (Sigma–Aldrich).

    Article Title: Subunit Architecture of the Golgi Dsc E3 Ligase Required for Sterol Regulatory Element-binding Protein (SREBP) Cleavage in Fission Yeast
    Article Snippet: .. General materials were obtained from Fisher and Sigma with the following exceptions: yeast extract, peptone, and agar from BD Biosciences; oligonucleotides were from Integrated DNA Technologies; digitonin were from EMD Chemicals; dithiobis[succinimidyl propionate] and EZ-link plus activated peroxidase were from Thermo Scientific; peptides N -glycosidase (PNGase) F and prestained protein standards were from New England Biolabs; isopropyl-1-thio-β-galactopyranoside and NativeMark unstained protein standard were from Invitrogen; Native PAGE materials were from Novex; MagneGST was from Promega; alkaline phosphatase, Complete EDTA-free protease inhibitor, mouse anti-GFP antibody, and anti-HA monoclonal 12CA5 IgG were from Roche Applied Science; mouse anti-ubiquitin monoclonal P4D1 IgG and anti-Myc monoclonal 9E10 IgG were from Santa Cruz Biotechnology; horseradish peroxidase-conjugated, affinity-purified donkey anti-rabbit and anti-mouse IgG were from Jackson ImmunoResearch Laboratories; protein A-agarose was from Repligen; and In vivo 2 400 Hypoxic Work station was from Biotrace. ..

    In Vivo:

    Article Title: Subunit Architecture of the Golgi Dsc E3 Ligase Required for Sterol Regulatory Element-binding Protein (SREBP) Cleavage in Fission Yeast
    Article Snippet: .. General materials were obtained from Fisher and Sigma with the following exceptions: yeast extract, peptone, and agar from BD Biosciences; oligonucleotides were from Integrated DNA Technologies; digitonin were from EMD Chemicals; dithiobis[succinimidyl propionate] and EZ-link plus activated peroxidase were from Thermo Scientific; peptides N -glycosidase (PNGase) F and prestained protein standards were from New England Biolabs; isopropyl-1-thio-β-galactopyranoside and NativeMark unstained protein standard were from Invitrogen; Native PAGE materials were from Novex; MagneGST was from Promega; alkaline phosphatase, Complete EDTA-free protease inhibitor, mouse anti-GFP antibody, and anti-HA monoclonal 12CA5 IgG were from Roche Applied Science; mouse anti-ubiquitin monoclonal P4D1 IgG and anti-Myc monoclonal 9E10 IgG were from Santa Cruz Biotechnology; horseradish peroxidase-conjugated, affinity-purified donkey anti-rabbit and anti-mouse IgG were from Jackson ImmunoResearch Laboratories; protein A-agarose was from Repligen; and In vivo 2 400 Hypoxic Work station was from Biotrace. ..

    Article Title: Lipid-Modified Azurin of Neisseria gonorrhoeae Is Not Surface Exposed and Does Not Interact With the Nitrite Reductase AniA
    Article Snippet: Paragraph title: In vivo Cross-Linking ... Dithiobis(succinimidyl propionate) (DSP; Sigma) was hydrated to 25 mM in dimethylsulfoxide and added to 100 μL of each suspension to 0, 0.1, 0.25, or 0.4 mM.

    Labeling:

    Article Title: Influence of KDEL on the Fate of Trimeric or Assembly-Defective Phaseolin
    Article Snippet: Protoplast (∼300,000, either unlabeled or radioactively labeled with ProMix for 3 hr) were pelleted by addition of 3 volumes of ice-cold W5 medium (154 mM NaCl, 5 mM KCl, 125 mM CaCl2 ·2H2 O, 5 mM glucose) followed by centrifugation at 60 g for 10 min at 4°C and washed once again with W5, leaving 50 μL to cover the pellet. .. Eight microliters of anhydrous DMSO supplemented or not with 12.5 mM dithiobis(succinimidyl propionate) (DSP; Sigma) were added, and the sample was incubated 30 min on ice with occasional gentle mixing.

    Purification:

    Article Title: Kinetic studies on the interactions of heparin and complement proteins using surface plasmon resonance
    Article Snippet: Porcine intestinal heparin (sodium salt) and 3, 3′-dithio-bis(propionic acid N -hydroxysuccinimide ester) (DTSP-NHS) were from Sigma (St. Louis, MO, USA). mPEG-SH and HCl·H2 N-PEG-COOH were from Nektar (Huntsville, AL, USA). .. Purified human complement proteins: C1 (MW 774 KD), C2 (MW 93 KD) and C3 (MW 190 KD) were from Calbiochem (La Jolla, CA) and are functionally pure; C5 (MW 190 KD, 1 mg/ml in PBS), C6 (MW 128 KD, 1 mg/ml in PBS), C7 (MW 121 KD, 1 mg/ml in PBS), C8 (MW 163 KD, 1 mg/ml in PBS), C9 (MW 79 KD, 1 mg/ml in PBS), factor I (MW 88 KD, 1.8 mg/ml in PBS), factor P (MW 220 KD, 1.0 mg/ml in PBS), factor B (MW 85.5 KD, 1.09 mg/ml in PBS pH 7.2), factor H (MW 150 KD, 1.05 mg/ml in PBS), and factor D (24 KD, 0.1–0.12 mg/ml in PBS pH 7.2) were all purchased from QUIDEL Corp (San Diego, CA, USA).

    Concentration Assay:

    Article Title: Generation and Characterization of Antibodies against Opioid Receptors from Zebrafish
    Article Snippet: GST fusion proteins were eluted from the beads with a solution containing 0.1 M glycine pH 2.5, which was later on neutralized with 1 M Tris pH 9.5 and supplemented with NaCl to a final concentration of 20 mM. .. To generate the affinity columns to purify antibodies, recombinant proteins bound to glutathione beads were covalently crosslinked with dithiobis(succinimidyl propionate) reagent (Sigma-Aldrich, St. Louis, MO, USA) following the instructions of the manufacturer.

    Incubation:

    Article Title: Syntrophin-dependent expression and localization of Aquaporin-4 water channel protein
    Article Snippet: .. After a 10-min 1,000 × g spin at 4°C, supernatants were incubated 30 min at 4°C with 150 μM dithiobis(succinimidyl propionate) (Sigma–Aldrich). ..

    Article Title: Influence of KDEL on the Fate of Trimeric or Assembly-Defective Phaseolin
    Article Snippet: .. Eight microliters of anhydrous DMSO supplemented or not with 12.5 mM dithiobis(succinimidyl propionate) (DSP; Sigma) were added, and the sample was incubated 30 min on ice with occasional gentle mixing. .. Homogenation and immunoprecipitation were performed as described above, using protoplast homogenation buffer and immunoprecipitation buffer at pH 8.0 instead of 7.5 to increase the stability of DSP.

    Article Title: Generation and Characterization of Antibodies against Opioid Receptors from Zebrafish
    Article Snippet: The supernatant was incubated with glutathione beads overnight at 4 °C. .. To generate the affinity columns to purify antibodies, recombinant proteins bound to glutathione beads were covalently crosslinked with dithiobis(succinimidyl propionate) reagent (Sigma-Aldrich, St. Louis, MO, USA) following the instructions of the manufacturer.

    Article Title: Lipid-Modified Azurin of Neisseria gonorrhoeae Is Not Surface Exposed and Does Not Interact With the Nitrite Reductase AniA
    Article Snippet: Dithiobis(succinimidyl propionate) (DSP; Sigma) was hydrated to 25 mM in dimethylsulfoxide and added to 100 μL of each suspension to 0, 0.1, 0.25, or 0.4 mM. .. Cross-linking reactions were incubated at 37°C and 5% CO2 for 30 min and quenched by the addition of 50 mM Tris-HCl pH 8.0 and incubation at room temperature for 10 min.

    Article Title: Nuclear-resident RIG-I senses viral replication inducing antiviral immunity
    Article Snippet: A549 or HEK293T cells were infected with indicated viruses or RNP reconstituted followed by crosslinking in PBS (−/−) containing 1 mM dithiobis[succinimidyl propionate] (DSP, Sigma) for 30 min on ice. .. The nuclear extracts were further pre-cleared with 20 μL protein G Dynabeads (Invitrogen) at 4 °C for 30 min. For co-immunoprecipitation, 35 μL protein G Dynabeads were conjugated with 3 μg of either mouse IgG1 isotype control (CST) or mouse anti-RIG-I antibody (1C3, Millipore) at 4 °C for 1 h. Pre-cleared lysates were then incubated with the conjugated beads at 4 °C for 16 h. On the next day, the beads were washed four times with the wash buffer (50 mM Tris-HCl, pH 7.4, 150 mM NaCl, 1% NP-40, 1 mM EDTA) and eluted in 1× Laemmli buffer containing 355 mM β-mercaptoethanol.

    other:

    Article Title: Anti-angiogenic effects of bumetanide revealed by DCE-MRI with a biodegradable macromolecular contrast agent in a colon cancer model
    Article Snippet: Diethylenetriamine (DETA), [2-(tert-butoxycarbonyloxyimino)-2-phenylacetonitrile] (BOC-ON), N,N-diisopropylethylamine (DIPEA), formic acid, sodium hydroxide (NaOH), 3,3′-dithiobis(sulfosuccinimidylpropionate) (DTSSP), and gadolinium(III) acetate were all purchased from Sigma-Aldrich (St. Louis, MO).

    Article Title: A Miniaturized Impedimetric Immunosensor for the Competitive Detection of Adrenocorticotropic Hormone
    Article Snippet: Streptavidin-conjugated alkaline phosphatase (Streptavidin-ALP), disodium 5-bromo-4-chloro-3-indolyl phosphate (BCIP), 3,3′-dithiobis[sulfosuccinimidylpropionate] (DTSSP) and ethanolamine were purchased from Sigma-Aldrich (Oakville, ON, Canada).

    Immunoprecipitation:

    Article Title: Influence of KDEL on the Fate of Trimeric or Assembly-Defective Phaseolin
    Article Snippet: Eight microliters of anhydrous DMSO supplemented or not with 12.5 mM dithiobis(succinimidyl propionate) (DSP; Sigma) were added, and the sample was incubated 30 min on ice with occasional gentle mixing. .. Homogenation and immunoprecipitation were performed as described above, using protoplast homogenation buffer and immunoprecipitation buffer at pH 8.0 instead of 7.5 to increase the stability of DSP.

    Infection:

    Article Title: Nuclear-resident RIG-I senses viral replication inducing antiviral immunity
    Article Snippet: .. A549 or HEK293T cells were infected with indicated viruses or RNP reconstituted followed by crosslinking in PBS (−/−) containing 1 mM dithiobis[succinimidyl propionate] (DSP, Sigma) for 30 min on ice. .. Cells were subjected to cellular fractionation as described elsewhere and the nuclear pellets were extracted at 4 °C for 30 min with a buffer containing 50 mM Tris-HCl (pH 7.4), 200 mM NaCl, 1% NP-40, 1 mM EDTA, 50 μg/mL RNase A, and 1× cOmplete protease inhibitors (Roche).

    Expressing:

    Article Title: Generation and Characterization of Antibodies against Opioid Receptors from Zebrafish
    Article Snippet: The culture was then diluted 1/100 with fresh media and allowed to grow at 37 °C to the exponential phase (Optic Density600 ≈ 0.4–0.6), when the expression of recombinant protein was induced with IPTG (0.1 mM) for 3 h. Bacteria were harvested, resuspended in ice-cold phosphate-buffered saline supplemented with lysozyme and protease inhibitors and sonicated to break the cells. .. To generate the affinity columns to purify antibodies, recombinant proteins bound to glutathione beads were covalently crosslinked with dithiobis(succinimidyl propionate) reagent (Sigma-Aldrich, St. Louis, MO, USA) following the instructions of the manufacturer.

    Cell Fractionation:

    Article Title: Nuclear-resident RIG-I senses viral replication inducing antiviral immunity
    Article Snippet: A549 or HEK293T cells were infected with indicated viruses or RNP reconstituted followed by crosslinking in PBS (−/−) containing 1 mM dithiobis[succinimidyl propionate] (DSP, Sigma) for 30 min on ice. .. Cells were subjected to cellular fractionation as described elsewhere and the nuclear pellets were extracted at 4 °C for 30 min with a buffer containing 50 mM Tris-HCl (pH 7.4), 200 mM NaCl, 1% NP-40, 1 mM EDTA, 50 μg/mL RNase A, and 1× cOmplete protease inhibitors (Roche).

    Sonication:

    Article Title: Generation and Characterization of Antibodies against Opioid Receptors from Zebrafish
    Article Snippet: Triton X-100 was added to the sonicated solution and centrifugation was performed to remove the debris. .. To generate the affinity columns to purify antibodies, recombinant proteins bound to glutathione beads were covalently crosslinked with dithiobis(succinimidyl propionate) reagent (Sigma-Aldrich, St. Louis, MO, USA) following the instructions of the manufacturer.

    Affinity Purification:

    Article Title: Subunit Architecture of the Golgi Dsc E3 Ligase Required for Sterol Regulatory Element-binding Protein (SREBP) Cleavage in Fission Yeast
    Article Snippet: .. General materials were obtained from Fisher and Sigma with the following exceptions: yeast extract, peptone, and agar from BD Biosciences; oligonucleotides were from Integrated DNA Technologies; digitonin were from EMD Chemicals; dithiobis[succinimidyl propionate] and EZ-link plus activated peroxidase were from Thermo Scientific; peptides N -glycosidase (PNGase) F and prestained protein standards were from New England Biolabs; isopropyl-1-thio-β-galactopyranoside and NativeMark unstained protein standard were from Invitrogen; Native PAGE materials were from Novex; MagneGST was from Promega; alkaline phosphatase, Complete EDTA-free protease inhibitor, mouse anti-GFP antibody, and anti-HA monoclonal 12CA5 IgG were from Roche Applied Science; mouse anti-ubiquitin monoclonal P4D1 IgG and anti-Myc monoclonal 9E10 IgG were from Santa Cruz Biotechnology; horseradish peroxidase-conjugated, affinity-purified donkey anti-rabbit and anti-mouse IgG were from Jackson ImmunoResearch Laboratories; protein A-agarose was from Repligen; and In vivo 2 400 Hypoxic Work station was from Biotrace. ..

    Recombinant:

    Article Title: Generation and Characterization of Antibodies against Opioid Receptors from Zebrafish
    Article Snippet: .. To generate the affinity columns to purify antibodies, recombinant proteins bound to glutathione beads were covalently crosslinked with dithiobis(succinimidyl propionate) reagent (Sigma-Aldrich, St. Louis, MO, USA) following the instructions of the manufacturer. .. Immunization of Rabbits Female rabbits (New Zealand) were initially immunized with 0.5 mg of the recombinant protein mixed with complete Freund adjuvant.

    Clear Native PAGE:

    Article Title: Subunit Architecture of the Golgi Dsc E3 Ligase Required for Sterol Regulatory Element-binding Protein (SREBP) Cleavage in Fission Yeast
    Article Snippet: .. General materials were obtained from Fisher and Sigma with the following exceptions: yeast extract, peptone, and agar from BD Biosciences; oligonucleotides were from Integrated DNA Technologies; digitonin were from EMD Chemicals; dithiobis[succinimidyl propionate] and EZ-link plus activated peroxidase were from Thermo Scientific; peptides N -glycosidase (PNGase) F and prestained protein standards were from New England Biolabs; isopropyl-1-thio-β-galactopyranoside and NativeMark unstained protein standard were from Invitrogen; Native PAGE materials were from Novex; MagneGST was from Promega; alkaline phosphatase, Complete EDTA-free protease inhibitor, mouse anti-GFP antibody, and anti-HA monoclonal 12CA5 IgG were from Roche Applied Science; mouse anti-ubiquitin monoclonal P4D1 IgG and anti-Myc monoclonal 9E10 IgG were from Santa Cruz Biotechnology; horseradish peroxidase-conjugated, affinity-purified donkey anti-rabbit and anti-mouse IgG were from Jackson ImmunoResearch Laboratories; protein A-agarose was from Repligen; and In vivo 2 400 Hypoxic Work station was from Biotrace. ..

    SDS Page:

    Article Title: Lipid-Modified Azurin of Neisseria gonorrhoeae Is Not Surface Exposed and Does Not Interact With the Nitrite Reductase AniA
    Article Snippet: Dithiobis(succinimidyl propionate) (DSP; Sigma) was hydrated to 25 mM in dimethylsulfoxide and added to 100 μL of each suspension to 0, 0.1, 0.25, or 0.4 mM. .. SDS-PAGE separation was performed at 4°C and 150 V followed by immunoblotting.

    Article Title: Kinetic studies on the interactions of heparin and complement proteins using surface plasmon resonance
    Article Snippet: Porcine intestinal heparin (sodium salt) and 3, 3′-dithio-bis(propionic acid N -hydroxysuccinimide ester) (DTSP-NHS) were from Sigma (St. Louis, MO, USA). mPEG-SH and HCl·H2 N-PEG-COOH were from Nektar (Huntsville, AL, USA). .. Factors P, B, H, D and I were ≥85% and the remaining complement proteins from QUIDEL were ≥95% pure by SDS-PAGE.

    Molecular Weight:

    Article Title: Seedless gold nanostars with seed-like advantages for biosensing applications
    Article Snippet: .. Materials and instrumentation Hydrochloroauric acid (HAuCl4 ), glucose oxidase (GOx), trisodium citrate, silver nitrate (AgNO3 ), ascorbic acid, sodium chloride (NaCl), polyvinylpyrrolidone (PVP) (molecular weight 10 000), hydrochloric acid (HCl), glucose, 3 3′-dithiobis (sulfosuccinimidyl propionate) (DTSSP), 2-(N-morpholino)ethanesulfonic acid (MES) at pH 6, (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) acid (HEPES) at pH 7.4, Tris-acetate-EDTA (TAE) at pH 8, bovine serum albumin (BSA), ethanolamine and phosphate buffered saline (PBS) at pH 7.4 were all purchased from Sigma-Aldrich, South Africa. ..

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