3 agei  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier
Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    New England Biolabs 3 agei
    3 Agei, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/3 agei/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    3 agei - by Bioz Stars, 2020-04
    86/100 stars

    Images

    Related Articles

    Dominant Negative Mutation:

    Article Title: TLR4 activation of TRPC6-dependent calcium signaling mediates endotoxin-induced lung vascular permeability and inflammation
    Article Snippet: Dominant-negative (DN)-TRPC6 mutant (T. Sharma, University of Illinois, Chicago, IL) was generated by deleting LFW sequence (Δ678-680 aa) from full-length TRPC6 cDNA. .. In the second step of PCR, a final PCR product, fragment C was generated by combining fragments A and B together as PCR DNA templates with primer pair GFP-494-F and YFP-820-R. Fragment C was digested with restriction enzymes 5′-EcoR1 and 3′-AgeI (New England Biolabs, Inc.) and ligated to the pEYFP-N1 vector (BD) also digested at the same restriction enzyme sites.

    Transfection:

    Article Title: TLR4 activation of TRPC6-dependent calcium signaling mediates endotoxin-induced lung vascular permeability and inflammation
    Article Snippet: Paragraph title: Transfection of cells. ... In the second step of PCR, a final PCR product, fragment C was generated by combining fragments A and B together as PCR DNA templates with primer pair GFP-494-F and YFP-820-R. Fragment C was digested with restriction enzymes 5′-EcoR1 and 3′-AgeI (New England Biolabs, Inc.) and ligated to the pEYFP-N1 vector (BD) also digested at the same restriction enzyme sites.

    Mutagenesis:

    Article Title: TLR4 activation of TRPC6-dependent calcium signaling mediates endotoxin-induced lung vascular permeability and inflammation
    Article Snippet: Dominant-negative (DN)-TRPC6 mutant (T. Sharma, University of Illinois, Chicago, IL) was generated by deleting LFW sequence (Δ678-680 aa) from full-length TRPC6 cDNA. .. In the second step of PCR, a final PCR product, fragment C was generated by combining fragments A and B together as PCR DNA templates with primer pair GFP-494-F and YFP-820-R. Fragment C was digested with restriction enzymes 5′-EcoR1 and 3′-AgeI (New England Biolabs, Inc.) and ligated to the pEYFP-N1 vector (BD) also digested at the same restriction enzyme sites.

    Polymerase Chain Reaction:

    Article Title: TLR4 activation of TRPC6-dependent calcium signaling mediates endotoxin-induced lung vascular permeability and inflammation
    Article Snippet: .. In the second step of PCR, a final PCR product, fragment C was generated by combining fragments A and B together as PCR DNA templates with primer pair GFP-494-F and YFP-820-R. Fragment C was digested with restriction enzymes 5′-EcoR1 and 3′-AgeI (New England Biolabs, Inc.) and ligated to the pEYFP-N1 vector (BD) also digested at the same restriction enzyme sites. ..

    Generated:

    Article Title: TLR4 activation of TRPC6-dependent calcium signaling mediates endotoxin-induced lung vascular permeability and inflammation
    Article Snippet: .. In the second step of PCR, a final PCR product, fragment C was generated by combining fragments A and B together as PCR DNA templates with primer pair GFP-494-F and YFP-820-R. Fragment C was digested with restriction enzymes 5′-EcoR1 and 3′-AgeI (New England Biolabs, Inc.) and ligated to the pEYFP-N1 vector (BD) also digested at the same restriction enzyme sites. ..

    Sequencing:

    Article Title: TLR4 activation of TRPC6-dependent calcium signaling mediates endotoxin-induced lung vascular permeability and inflammation
    Article Snippet: Dominant-negative (DN)-TRPC6 mutant (T. Sharma, University of Illinois, Chicago, IL) was generated by deleting LFW sequence (Δ678-680 aa) from full-length TRPC6 cDNA. .. In the second step of PCR, a final PCR product, fragment C was generated by combining fragments A and B together as PCR DNA templates with primer pair GFP-494-F and YFP-820-R. Fragment C was digested with restriction enzymes 5′-EcoR1 and 3′-AgeI (New England Biolabs, Inc.) and ligated to the pEYFP-N1 vector (BD) also digested at the same restriction enzyme sites.

    Plasmid Preparation:

    Article Title: TLR4 activation of TRPC6-dependent calcium signaling mediates endotoxin-induced lung vascular permeability and inflammation
    Article Snippet: .. In the second step of PCR, a final PCR product, fragment C was generated by combining fragments A and B together as PCR DNA templates with primer pair GFP-494-F and YFP-820-R. Fragment C was digested with restriction enzymes 5′-EcoR1 and 3′-AgeI (New England Biolabs, Inc.) and ligated to the pEYFP-N1 vector (BD) also digested at the same restriction enzyme sites. ..

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86
    New England Biolabs 3 agei
    3 Agei, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/3 agei/product/New England Biolabs
    Average 86 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    3 agei - by Bioz Stars, 2020-04
    86/100 stars
      Buy from Supplier

    Image Search Results