3 3 diaminobenzidine (Thermo Fisher)

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Diaminobenzidine DAB Histochemistry Kit 3

Description:

The DAB Histochemistry Kit for biotinylated probes contains sufficient materials to stain approximately 200 slides using horseradish peroxidase HRP and the HRP substrate DAB The brown colored DAB reaction product can be visualized by bright fieldd light microscopy or following osmication by electron microscopy

Catalog Number:

d22187

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None

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Antibodies Secondary Detection Reagents

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Structured Review

Thermo Fisher 3 3 diaminobenzidine
The DAB Histochemistry Kit for biotinylated probes contains sufficient materials to stain approximately 200 slides using horseradish peroxidase HRP and the HRP substrate DAB The brown colored DAB reaction product can be visualized by bright fieldd light microscopy or following osmication by electron microscopy
https://www.bioz.com/result/3 3 diaminobenzidine/product/Thermo Fisher
Average 96 stars, based on 1 article reviews
Price from $9.99 to $1999.99

3 3 diaminobenzidine - by Bioz Stars, 2021-03

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In Situ Hybridization:

Article Title: Effects of chronic caffeine exposure during adolescence and subsequent acute caffeine challenge during adulthood on rat brain serotonergic systems
Article Snippet: Dehydrated sections were exposed to x-ray film (BioMax® MR; Eastman Kodak, Rochester, NY, USA) for 3 ( tph2 ) or 21 ( slc22a3) days prior to film development. .. Oligonucleotide-based in situ hybridization histochemistry ( htr1a and slc6a4 ) Previously published methods were used for in situ hybridization histochemistry using oligonucleotide probes ( ). .. To detect htr1a mRNA, two synthetic anti-sense oligonucleotides, one 49-base oligonucleotide (5′-ACG AAG TTC CTA AGC TGG TGC CTG CTC CCT TCT TTT CCA CCT TCC TGA C-3′, Integrated DNA Technologies, Coralville, IA, USA) complementary to bases 810–858 of rat htrla mRNA and one 47-base oligonucleotide (5′-GCC TCA CTG CCC CAT TAG TGC ACG GAG TCC CCA CCG CCC TGT TCT CA-3′, Integrated DNA Technologies) complementary to bases 923–969 of rat htr1a mRNA, were used ( ).

Immunohistochemistry:

Article Title: Lentiviral Vector Induced Modeling of High-Grade Spinal Cord Glioma in Minipigs
Article Snippet: Qualitatively, H & E sections were evaluated by a board-certified clinical neuropathologist (S.N. and P.C.) under wide-field microscopy blinded to the study design. .. Immunohistochemical stains were performed with primary antibodies for GFAP (Dako, Z0034), Olig2 (abcam, ab109186), Ki67 (abcam, ab15580) with appropriate secondaries for subsequent diaminobenzidine (DAB) and hematoxylin counterstaining (Thermo Scientific, Autostainer 480S). .. Immunohistochemical stains for glial markers (Olig2, GFAP) were qualitatively evaluated.

Incubation:

Article Title: Effects of Electrospun Fibrous Membranes of PolyCaprolactone and Chitosan/Poly(Ethylene Oxide) on Mouse Acute Skin Lesions
Article Snippet: After this step, the samples were washed with 0.05M PBS and incubated with anti-Rabbit secondary antibody (Thermo Fisher, Waltham, MA, USA), 31460, dilution 1:400) for 60 min, at room temperature. .. Immunoactivity was visualized by incubation with Diaminobenzidine (DAB) for 1 min in the dark. .. The sections were stained with Mayer’s Hematoxylin for 15 s, and finally, the slides were assembled to be analyzed with Nikon DS-Ri1 (Melville, NY, USA).

other:

Article Title: Ameliorative role of antioxidant supplementation on sodium-arsenite induced adverse effects on the developing rat cerebellum
Article Snippet: 3′,3’ diaminobenzidine (DAB) was used as chromogen for visualizing immunoreactivity.

Staining:

Article Title: Fluorescent multiplexing of 3D spheroids: Analysis of biomarkers using automated immunohistochemistry staining platform and multispectral imaging, et al. Fluorescent multiplexing of 3D spheroids: Analysis of biomarkers using automated immunohistochemistry staining platform and multispectral imaging
Article Snippet: For chromogenic (DAB) staining, some slides were developed with the ultraView Universal DAB Detection Kit (Cat. 760‐500) or OptiView DAB IHC Detection Kit (Cat. 760‐700), with or without amplification with OptiView Amplification Kit (760‐099), according to manufacturer's specifications. .. The biomarkers and antibodies for automated chromogenic DAB staining are listed in Table . .. 2.5 Automated IF multiplex staining processFluorescent 4‐plex staining of 3D spheroids and aggregates were performed using the U Discovery 5‐Plex RUO staining procedure, which utilizes tyramide signal amplification (TSA)., The assay was run on the VENTANA DISCOVERY ULTRA automated staining platform (Roche Tissue Diagnostics) using VENTANA/Roche reagents, except where noted.

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3 3 diaminobenzidine dab (Thermo Fisher)

Thermo Fisher 3 3 diaminobenzidine dab

Ultrastructural immunocytochemical evidence for mast cell-derived gonadotropin-releasing hormone (GnRH) in neurons. (A) <t>3,3′</t> <t>diaminobenzidine</t> <t>(DAB),</t> used to detect sites of antibody binding, forms a flocculent deposit (which can obscure finer ultrastructural detail) in some (white arrow) but not all granules within the mast cell. DAB deposits are also present in two adjacent neurons (N 1 and N 2 ; arrowheads). There are multiple layers of mast cell filopodia cut in both longitudinal and cross section (double arrows). Some of these are associated with DAB deposits (long arrows). (B) In this plane of section the mast cell (MC) has few granules. Within the neuron (N), small deposits of immunoreactive material are found associated with vesicles (arrows). Some of these vesicles are close to the Golgi apparatus (g). DAB is also associated with the surface of filopodia (arrowhead). Double arrows indicate the characteristic multiple filopodial layers of the MC.

3 3 Diaminobenzidine Dab, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/3 3 diaminobenzidine dab/product/Thermo Fisher
Average 99 stars, based on 1 article reviews
Price from $9.99 to $1999.99

3 3 diaminobenzidine dab - by Bioz Stars, 2021-03

99/100 stars

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Image Search Results

Ultrastructural immunocytochemical evidence for mast cell-derived gonadotropin-releasing hormone (GnRH) in neurons. (A) 3,3′ diaminobenzidine (DAB), used to detect sites of antibody binding, forms a flocculent deposit (which can obscure finer ultrastructural detail) in some (white arrow) but not all granules within the mast cell. DAB deposits are also present in two adjacent neurons (N 1 and N 2 ; arrowheads). There are multiple layers of mast cell filopodia cut in both longitudinal and cross section (double arrows). Some of these are associated with DAB deposits (long arrows). (B) In this plane of section the mast cell (MC) has few granules. Within the neuron (N), small deposits of immunoreactive material are found associated with vesicles (arrows). Some of these vesicles are close to the Golgi apparatus (g). DAB is also associated with the surface of filopodia (arrowhead). Double arrows indicate the characteristic multiple filopodial layers of the MC.

Journal: The European Journal of Neuroscience

Article Title: Central nervous system neurons acquire mast cell products via transgranulation

doi: 10.1111/j.1460-9568.2005.04429.x

Figure Lengend Snippet: Ultrastructural immunocytochemical evidence for mast cell-derived gonadotropin-releasing hormone (GnRH) in neurons. (A) 3,3′ diaminobenzidine (DAB), used to detect sites of antibody binding, forms a flocculent deposit (which can obscure finer ultrastructural detail) in some (white arrow) but not all granules within the mast cell. DAB deposits are also present in two adjacent neurons (N 1 and N 2 ; arrowheads). There are multiple layers of mast cell filopodia cut in both longitudinal and cross section (double arrows). Some of these are associated with DAB deposits (long arrows). (B) In this plane of section the mast cell (MC) has few granules. Within the neuron (N), small deposits of immunoreactive material are found associated with vesicles (arrows). Some of these vesicles are close to the Golgi apparatus (g). DAB is also associated with the surface of filopodia (arrowhead). Double arrows indicate the characteristic multiple filopodial layers of the MC.

Article Snippet: The first made use of conventional staining with 3,3′ diaminobenzidine (DAB) (Immunopure DAB, Pierce) and the second, a silver intensification followed by gold toning of the DAB product (not shown).

Techniques: Derivative Assay, Binding Assay

Cell surface expression of B5 in porcine cells. (A) Cartoon of the predicted structure of B5 as an integral membrane protein with a predicted C-terminal α-helix. The C terminus and N terminus are labeled. (B) Cells transiently transfected with pB5-myc or vector only (pcDNA-myc) fixed at 48 h posttransfection and stained with anti-myc antibody, a secondary, peroxidase-conjugated antibody and diaminobenzidine substrate. (C) FACS of SK6-A7 cells transiently transfected with pcDNA vector (left) or pB5-myc (right) and stained with anti-myc (9E10) and a secondary, FITC-conjugated antibody (gray lines). Black peaks are cells in the absence of primary anti-myc antibody. (D) FACS of the indicated stable porcine cell line exposed to anti-Xpress and a secondary, FITC-conjugated antibody. Cells were permeabilized (P) with methanol-acetone or not permeabilized (unpermeabilized [U]) before staining. Neo is a vector-only-transformed cell line. NB5 is a stable cell line made with an N-terminal Xpress epitope-tagged B5. HB1-9 is a stable untagged HVEM-expressing porcine cell line. (E and F) NB5 and HB1-9 cells surface labeled with biotin and lysed as described in Materials and Methods. Lysates normalized for protein concentration were immunoprecipitated with preimmune serum (Pre) or with anti-HVEM polyclonal serum (HVEM), or anti-Xpress monoclonal antibody (XPRS). Proteins visualized in Western blots hybridized with streptavidin-conjugated peroxidase and enhanced chemiluminescence substrate (Amersham) (E) or anti-Xpress monoclonal antibody and alkaline phosphatase-conjugated anti-mouse secondary antibody and NBT/BCIP (F). The positions of molecular mass markers (in kilodaltons) are shown to the left of the gels. The arrow points to B5 at about 43 kDa.

Journal: Journal of Virology

Article Title: A New Class of Receptor for Herpes Simplex Virus Has Heptad Repeat Motifs That Are Common to Membrane Fusion Proteins

doi: 10.1128/JVI.79.12.7419-7430.2005

Figure Lengend Snippet: Cell surface expression of B5 in porcine cells. (A) Cartoon of the predicted structure of B5 as an integral membrane protein with a predicted C-terminal α-helix. The C terminus and N terminus are labeled. (B) Cells transiently transfected with pB5-myc or vector only (pcDNA-myc) fixed at 48 h posttransfection and stained with anti-myc antibody, a secondary, peroxidase-conjugated antibody and diaminobenzidine substrate. (C) FACS of SK6-A7 cells transiently transfected with pcDNA vector (left) or pB5-myc (right) and stained with anti-myc (9E10) and a secondary, FITC-conjugated antibody (gray lines). Black peaks are cells in the absence of primary anti-myc antibody. (D) FACS of the indicated stable porcine cell line exposed to anti-Xpress and a secondary, FITC-conjugated antibody. Cells were permeabilized (P) with methanol-acetone or not permeabilized (unpermeabilized [U]) before staining. Neo is a vector-only-transformed cell line. NB5 is a stable cell line made with an N-terminal Xpress epitope-tagged B5. HB1-9 is a stable untagged HVEM-expressing porcine cell line. (E and F) NB5 and HB1-9 cells surface labeled with biotin and lysed as described in Materials and Methods. Lysates normalized for protein concentration were immunoprecipitated with preimmune serum (Pre) or with anti-HVEM polyclonal serum (HVEM), or anti-Xpress monoclonal antibody (XPRS). Proteins visualized in Western blots hybridized with streptavidin-conjugated peroxidase and enhanced chemiluminescence substrate (Amersham) (E) or anti-Xpress monoclonal antibody and alkaline phosphatase-conjugated anti-mouse secondary antibody and NBT/BCIP (F). The positions of molecular mass markers (in kilodaltons) are shown to the left of the gels. The arrow points to B5 at about 43 kDa.

Article Snippet: For immunostaining, cells at about 100% confluency were fixed with 2% paraformaldehyde-0.2% glutaraldehyde and stained with anti-myc (1:1,000) followed by anti-mouse secondary antibody and diaminobenzidine substrate (Lifetech).

Techniques: Expressing, Labeling, Transfection, Plasmid Preparation, Staining, FACS, Transformation Assay, Stable Transfection, Protein Concentration, Immunoprecipitation, Western Blot

Immunolocalization of ANG-(1–12). Paraformaldehyde-fixed, frozen brain sections ( n = 3 and 30 μm) were incubated with the ANG-(1–12) antibody to determine expression of ANG-(1–12) in dorsal medulla of Sprague-Dawley rats. 3,3′-Diaminobenzidine ( A – F ) and immunofluorescence ( G and H ) staining show ANG-(1–12) expression in various brainstem regions, including the NTS, in representative adjacent sections at ∼−13.8 mm caudal to bregma. ANG-(1–12) immunostaining was widely expressed in the medulla ( A and D ; 5×). Control sections (5×) were incubated with 40 μM ANG-(1–12) peptide together with the ANG-(1–12) antibody ( B ) or no ANG-(1–12) antibody ( C ). A higher magnification (40×) shows ANG-(1–12)-like immunoreactivity in cell bodies and fibers in the area postrema ( E ), NTS ( E ), and dorsal motor nucleus of the vagus ( F ). Adjacent sections were incubated with the ANG-(1–12) antibody ( G ) or no primary antibody ( H ) for immunofluorescence localization. ANG-(1–12) fluorescence is shown in green and nuclei in blue (40×). dmnX, Dorsal motor nucleus of the vagus; Cu, cuneate nucleus; Gr, nucleus gracilis; CC, central canal; HyG, hypoglossal nucleus; IO, inferior olivary nucleus. Scale bars: A – D , 100 μm, and E – H , 10 μm.

Journal: American Journal of Physiology - Heart and Circulatory Physiology

Article Title: Angiotensin-(1-12) requires angiotensin converting enzyme and AT1 receptors for cardiovascular actions within the solitary tract nucleus

doi: 10.1152/ajpheart.00345.2010

Figure Lengend Snippet: Immunolocalization of ANG-(1–12). Paraformaldehyde-fixed, frozen brain sections ( n = 3 and 30 μm) were incubated with the ANG-(1–12) antibody to determine expression of ANG-(1–12) in dorsal medulla of Sprague-Dawley rats. 3,3′-Diaminobenzidine ( A – F ) and immunofluorescence ( G and H ) staining show ANG-(1–12) expression in various brainstem regions, including the NTS, in representative adjacent sections at ∼−13.8 mm caudal to bregma. ANG-(1–12) immunostaining was widely expressed in the medulla ( A and D ; 5×). Control sections (5×) were incubated with 40 μM ANG-(1–12) peptide together with the ANG-(1–12) antibody ( B ) or no ANG-(1–12) antibody ( C ). A higher magnification (40×) shows ANG-(1–12)-like immunoreactivity in cell bodies and fibers in the area postrema ( E ), NTS ( E ), and dorsal motor nucleus of the vagus ( F ). Adjacent sections were incubated with the ANG-(1–12) antibody ( G ) or no primary antibody ( H ) for immunofluorescence localization. ANG-(1–12) fluorescence is shown in green and nuclei in blue (40×). dmnX, Dorsal motor nucleus of the vagus; Cu, cuneate nucleus; Gr, nucleus gracilis; CC, central canal; HyG, hypoglossal nucleus; IO, inferior olivary nucleus. Scale bars: A – D , 100 μm, and E – H , 10 μm.

Article Snippet: Color was developed with 3,3′-diaminobenzidine HCl/H2 O2 (Thermo Scientific).

Techniques: Incubation, Expressing, Immunofluorescence, Staining, Immunostaining, Fluorescence

Effects of MMDT treatment on airway remodeling in lung tissue (MLC2 immunohistochemistry). (a) Stainedmouse lung sections and (b) MLC2 area ( μ m 2 ). Mouse lung sections were stained for MLC2 detection. The sections were incubated at 4°C overnight with an anti-MLC2 goat polyclonal antibody (1 : 50). The slides were then incubated with avidin-biotin peroxidase complex, and color was developed using 3,3-diaminobenzidine tetrachloride. The arrows indicate MCL2 positive cells (magnification 200x). Normal control mice treated with PBS only (NC), CKA-challenged mice treated with PBS (CKA), CKA-challenged mice treated with 10 mg/kg of Montelukast (MK), CKA-challenged mice treated with 100 mg/kg of MMDT (MMDT 100 mg/kg), CKA-challenged mice treated with 200 mg/kg of MMDT (MMDT 200 mg/kg), and CKA-challenged mice treated with 400 mg/kg of MMDT (MMDT 400 mg/kg). The data are shown as the mean ± S.E.M. Statistical analysis was conducted by one-way ANOVA followed by the Newman-Keuls Multiple Comparison test (significantly different from NC, ** P

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: The Effects of Maekmoondong-Tang on Cockroach Extract-Induced Allergic Asthma

doi: 10.1155/2014/958965

Figure Lengend Snippet: Effects of MMDT treatment on airway remodeling in lung tissue (MLC2 immunohistochemistry). (a) Stainedmouse lung sections and (b) MLC2 area ( μ m 2 ). Mouse lung sections were stained for MLC2 detection. The sections were incubated at 4°C overnight with an anti-MLC2 goat polyclonal antibody (1 : 50). The slides were then incubated with avidin-biotin peroxidase complex, and color was developed using 3,3-diaminobenzidine tetrachloride. The arrows indicate MCL2 positive cells (magnification 200x). Normal control mice treated with PBS only (NC), CKA-challenged mice treated with PBS (CKA), CKA-challenged mice treated with 10 mg/kg of Montelukast (MK), CKA-challenged mice treated with 100 mg/kg of MMDT (MMDT 100 mg/kg), CKA-challenged mice treated with 200 mg/kg of MMDT (MMDT 200 mg/kg), and CKA-challenged mice treated with 400 mg/kg of MMDT (MMDT 400 mg/kg). The data are shown as the mean ± S.E.M. Statistical analysis was conducted by one-way ANOVA followed by the Newman-Keuls Multiple Comparison test (significantly different from NC, ** P

Article Snippet: After the slides were incubated with avidin-biotin peroxidase complex (ABC kit, Vestor Laboratories, CA, USA), the color was developed with 3,3′-diaminobenzidine tetrachloride (DAB; Zymed Laboratories, CA, USA).

Techniques: Immunohistochemistry, Staining, Incubation, Avidin-Biotin Assay, Mouse Assay