3 3 diaminobenzidine tetrahydrochloride  (Vector Laboratories)


Bioz Verified Symbol Vector Laboratories is a verified supplier
Bioz Manufacturer Symbol Vector Laboratories manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86
    Name:
    DAB Peroxidase HRP Substrate Kit with Nickel 3 3 diaminobenzidine
    Description:
    DAB Peroxidase Substrate Kit with Nickel provides greater sensitivity than conventional substrates Consistent and reliableIdeal for IHC ICC ISH and blotsHeat Stable Permanent mountingIdeal for single and multiple labeling Stock solutions supplied in convenient dropper bottles promoting ease of handlingNo wait times for mixing and dissolving powders or tablets Includes nickel to produce a gray black rather than brown reaction product One year expiry dateSufficient reagents to produce 300 ml of working solution DAB 3 3 diaminobenzidine HRP substrate produces a dark brown reaction product and can be used for both immunohistochemical and blotting applications DAB chromogen is effective as a single label or as a second color for multiple antigen labeling Because of its heat resistance DAB can be used in IHC ISH double labeling applications With the aid of imaging systems and software the spectral profile of DAB can be distinguished from our other proprietary enzyme substrates in applications where antigens are co localized Sections stained with DAB can also be viewed by darkfield and electron microscopy Slides developed with DAB can be dehydrated cleared and permanently mounted The DAB reaction product can be intensified with DAB Enhancing Solution H 2200 This kit contains stock solutions in convenient dropper bottles This kit includes nickel providing the option of changing the reaction product from brown to gray black and increasing sensitivity in blot applications Slides developed with DAB Ni should be dehydrated cleared and permanently mounted This product can also be used on blots This kit provides all of the necessary reagents to prepare about 300 ml of working solution When stored at 4C this kit is stable for one year
    Catalog Number:
    sk-4100
    Price:
    None
    Category:
    Proteins
    Size:
    1 Kit
    Buy from Supplier


    Structured Review

    Vector Laboratories 3 3 diaminobenzidine tetrahydrochloride
    DAB Peroxidase HRP Substrate Kit with Nickel 3 3 diaminobenzidine
    DAB Peroxidase Substrate Kit with Nickel provides greater sensitivity than conventional substrates Consistent and reliableIdeal for IHC ICC ISH and blotsHeat Stable Permanent mountingIdeal for single and multiple labeling Stock solutions supplied in convenient dropper bottles promoting ease of handlingNo wait times for mixing and dissolving powders or tablets Includes nickel to produce a gray black rather than brown reaction product One year expiry dateSufficient reagents to produce 300 ml of working solution DAB 3 3 diaminobenzidine HRP substrate produces a dark brown reaction product and can be used for both immunohistochemical and blotting applications DAB chromogen is effective as a single label or as a second color for multiple antigen labeling Because of its heat resistance DAB can be used in IHC ISH double labeling applications With the aid of imaging systems and software the spectral profile of DAB can be distinguished from our other proprietary enzyme substrates in applications where antigens are co localized Sections stained with DAB can also be viewed by darkfield and electron microscopy Slides developed with DAB can be dehydrated cleared and permanently mounted The DAB reaction product can be intensified with DAB Enhancing Solution H 2200 This kit contains stock solutions in convenient dropper bottles This kit includes nickel providing the option of changing the reaction product from brown to gray black and increasing sensitivity in blot applications Slides developed with DAB Ni should be dehydrated cleared and permanently mounted This product can also be used on blots This kit provides all of the necessary reagents to prepare about 300 ml of working solution When stored at 4C this kit is stable for one year
    https://www.bioz.com/result/3 3 diaminobenzidine tetrahydrochloride/product/Vector Laboratories
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    3 3 diaminobenzidine tetrahydrochloride - by Bioz Stars, 2021-03
    86/100 stars

    Images

    1) Product Images from "Up-Regulation of A1M/?1-Microglobulin in Skin by Heme and Reactive Oxygen Species Gives Protection from Oxidative Damage"

    Article Title: Up-Regulation of A1M/?1-Microglobulin in Skin by Heme and Reactive Oxygen Species Gives Protection from Oxidative Damage

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0027505

    Histochemical distribution and expression of A1M. A. The sections (x10) were stained for A1M using monoclonal mouse anti-A1M BN11.10 (10 µg/mL) followed by peroxidase/diaminobenzidine tetrahydrochloride incubation as described in Materials and Methods . B . Control sections (x10) incubated without primary antibody. C, D and E. Human skin explants (1×10×2 mm) or primary keratinocytes were cultured in serum-free medium as described in Materials and Methods . C. Skin explants were incubated with 20 µM (NH 4 )Fe(SO 4 ) 2 +200 µM ascorbate+40 µM H 2 O 2 ( = Fenton; white bar) or 20 µM heme (grey) for a period of 20 hours. D. Keratinocytes were incubated with 20 µM heme for a period of 3 (white) or 20 (grey) hours. Total RNA was extracted from homogenized cells, cDNA was prepared using reverse transcription and expression of A1M was analyzed using real-time PCR as described in Materials and Methods . A1M threshold cycle values were normalized against G3DPH and ΔΔCt was calculated by normalizing against control samples which were incubated with buffer only for the same time-periods. Consequently, ΔΔCt-values of all controls are zero and correspond to the baseline. E. Keratinocytes were cultured in serum-free medium as described in Materials and Methods . The cells were then incubated with 20 µM heme for a period of 3 (white) or 20 (grey) hours. The cells were homogenized and the A1M concentration was determined using RIA as described in Materials and Methods . The total protein concentrations were determined with Bradford protein assay as described in Materials and Methods . Results are from triplicate experiments and are presented as mean ± SEM. Statistical comparison with control cultures was made using Students t test. * P
    Figure Legend Snippet: Histochemical distribution and expression of A1M. A. The sections (x10) were stained for A1M using monoclonal mouse anti-A1M BN11.10 (10 µg/mL) followed by peroxidase/diaminobenzidine tetrahydrochloride incubation as described in Materials and Methods . B . Control sections (x10) incubated without primary antibody. C, D and E. Human skin explants (1×10×2 mm) or primary keratinocytes were cultured in serum-free medium as described in Materials and Methods . C. Skin explants were incubated with 20 µM (NH 4 )Fe(SO 4 ) 2 +200 µM ascorbate+40 µM H 2 O 2 ( = Fenton; white bar) or 20 µM heme (grey) for a period of 20 hours. D. Keratinocytes were incubated with 20 µM heme for a period of 3 (white) or 20 (grey) hours. Total RNA was extracted from homogenized cells, cDNA was prepared using reverse transcription and expression of A1M was analyzed using real-time PCR as described in Materials and Methods . A1M threshold cycle values were normalized against G3DPH and ΔΔCt was calculated by normalizing against control samples which were incubated with buffer only for the same time-periods. Consequently, ΔΔCt-values of all controls are zero and correspond to the baseline. E. Keratinocytes were cultured in serum-free medium as described in Materials and Methods . The cells were then incubated with 20 µM heme for a period of 3 (white) or 20 (grey) hours. The cells were homogenized and the A1M concentration was determined using RIA as described in Materials and Methods . The total protein concentrations were determined with Bradford protein assay as described in Materials and Methods . Results are from triplicate experiments and are presented as mean ± SEM. Statistical comparison with control cultures was made using Students t test. * P

    Techniques Used: Expressing, Staining, Incubation, Cell Culture, Real-time Polymerase Chain Reaction, Concentration Assay, Bradford Protein Assay

    Related Articles

    Immunohistochemistry:

    Article Title: Cell-based quantification of molecular biomarkers in histopathology specimens
    Article Snippet: Antibodies used for immunostaining included monoclonal mouse anti-human oestrogen receptor (ER), anti-human progesterone receptor (PR), anti-human Ki67, anti-epithelial membrane antigen (EMA), rabbit polyclonal anti-human epidermal growth factor receptor 2 (HER2) (Dako, Carpenteria, CA, USA), rabbit anti-phospho(p)-extracellular signal-related kinase (ERK), anti-p-S6 (Cell Signaling, Danvers, MA, USA), and mouse anti-multi-cytokeratin (CK) monoclonal antibodies (Vector Laboratories, Burlingame, CA, USA). .. ER, PR, Ki67, p-ERK and HER2 were detected by immunohistochemistry with biotinylated species-specific secondary antibodies, avidin-linked horseradish peroxidase (HRP) (ABC Kit) and 3,3-diaminobenzidine or SG Blue (Vector Laboratories) HRP chromogen substrate. .. CK, EMA and p-S6 immunostaining were detected by fluorescence, with the use of Zenon Alexa Fluor-488 mouse IgG1 labelling (Invitrogen, Carlsbad, CA, USA), fluorescently labelled secondary antibodies (Invitrogen) or the ABC fluorescence detection kit.

    Avidin-Biotin Assay:

    Article Title: Cell-based quantification of molecular biomarkers in histopathology specimens
    Article Snippet: Antibodies used for immunostaining included monoclonal mouse anti-human oestrogen receptor (ER), anti-human progesterone receptor (PR), anti-human Ki67, anti-epithelial membrane antigen (EMA), rabbit polyclonal anti-human epidermal growth factor receptor 2 (HER2) (Dako, Carpenteria, CA, USA), rabbit anti-phospho(p)-extracellular signal-related kinase (ERK), anti-p-S6 (Cell Signaling, Danvers, MA, USA), and mouse anti-multi-cytokeratin (CK) monoclonal antibodies (Vector Laboratories, Burlingame, CA, USA). .. ER, PR, Ki67, p-ERK and HER2 were detected by immunohistochemistry with biotinylated species-specific secondary antibodies, avidin-linked horseradish peroxidase (HRP) (ABC Kit) and 3,3-diaminobenzidine or SG Blue (Vector Laboratories) HRP chromogen substrate. .. CK, EMA and p-S6 immunostaining were detected by fluorescence, with the use of Zenon Alexa Fluor-488 mouse IgG1 labelling (Invitrogen, Carlsbad, CA, USA), fluorescently labelled secondary antibodies (Invitrogen) or the ABC fluorescence detection kit.

    Immunolabeling:

    Article Title: Connective Tissue Growth Factor Modulates Adult β-Cell Maturity and Proliferation to Promote β-Cell Regeneration in Mice
    Article Snippet: Imaging was with a ScanScope FL scanner (Aperio Technologies, Inc.) and quantified using Metamorph 6.1 (Molecular Devices). .. β-Cell Mass Ten to 12 slides per animal (1 to 2% of entire pancreas) were immunolabeled for insulin, visualized via the DAB Peroxidase Substrate Kit (Vector Laboratories), and counterstained with eosin. ..

    Incubation:

    Article Title: CRE/CREB-Driven Up-Regulation of Gene Expression by Chronic Social Stress in CRE-Luciferase Transgenic Mice: Reversal by Antidepressant Treatment
    Article Snippet: .. Sections were washed intensively and than incubated with rabbit anti goat antibody (DAKO, Hamburg, Germany, diluted 1∶100) for 4 h. After washing, sections were incubated with anti-rabbit-PAP (DAKO, diluted 1∶100 in blocking buffer) for 2 h. Luciferase immunoreactivity was developed by incubating the sections for 5 min in 50 mM Tris pH 7.2 with 0.6 mg/ml of 3,3′-diaminobenzidine (DAB) and 0.3 mg/ml H2 O2 (Vector Labs/Alexis Deutschland, Grünberg, Germany). ..

    Blocking Assay:

    Article Title: CRE/CREB-Driven Up-Regulation of Gene Expression by Chronic Social Stress in CRE-Luciferase Transgenic Mice: Reversal by Antidepressant Treatment
    Article Snippet: .. Sections were washed intensively and than incubated with rabbit anti goat antibody (DAKO, Hamburg, Germany, diluted 1∶100) for 4 h. After washing, sections were incubated with anti-rabbit-PAP (DAKO, diluted 1∶100 in blocking buffer) for 2 h. Luciferase immunoreactivity was developed by incubating the sections for 5 min in 50 mM Tris pH 7.2 with 0.6 mg/ml of 3,3′-diaminobenzidine (DAB) and 0.3 mg/ml H2 O2 (Vector Labs/Alexis Deutschland, Grünberg, Germany). ..

    Luciferase:

    Article Title: CRE/CREB-Driven Up-Regulation of Gene Expression by Chronic Social Stress in CRE-Luciferase Transgenic Mice: Reversal by Antidepressant Treatment
    Article Snippet: .. Sections were washed intensively and than incubated with rabbit anti goat antibody (DAKO, Hamburg, Germany, diluted 1∶100) for 4 h. After washing, sections were incubated with anti-rabbit-PAP (DAKO, diluted 1∶100 in blocking buffer) for 2 h. Luciferase immunoreactivity was developed by incubating the sections for 5 min in 50 mM Tris pH 7.2 with 0.6 mg/ml of 3,3′-diaminobenzidine (DAB) and 0.3 mg/ml H2 O2 (Vector Labs/Alexis Deutschland, Grünberg, Germany). ..

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 97
    Vector Laboratories 3 3 diaminobenzidine tetrahydrochloride
    Characteristics of CCs during in vitro expansion. (A) IHC staining for type I collagen and IF staining for type II collagen, CD29, and α-SMA were performed in CCs cultured in D, M, DF, or MF at passages 2 and 8 ( n = 5). To visualize total cells, nuclei were counterstained with hematoxylin and 4′,6-diamidino-2-phenylindole for IHC and IF, respectively. (B) Negative control experiments confirmed the specificities of the IHC and IF signals. Scale bar, 100 µm. DAB, <t>3,3′-diaminobenzidine;</t> anti-Rb, antirabbit immunoglobulin; anti-M, antimouse immunoglobulin; CCs, costal chondrocytes; IHC, immunohistochemical; IF, immunofluorescence.
    3 3 Diaminobenzidine Tetrahydrochloride, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/3 3 diaminobenzidine tetrahydrochloride/product/Vector Laboratories
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    3 3 diaminobenzidine tetrahydrochloride - by Bioz Stars, 2021-03
    97/100 stars
      Buy from Supplier

    Image Search Results


    Characteristics of CCs during in vitro expansion. (A) IHC staining for type I collagen and IF staining for type II collagen, CD29, and α-SMA were performed in CCs cultured in D, M, DF, or MF at passages 2 and 8 ( n = 5). To visualize total cells, nuclei were counterstained with hematoxylin and 4′,6-diamidino-2-phenylindole for IHC and IF, respectively. (B) Negative control experiments confirmed the specificities of the IHC and IF signals. Scale bar, 100 µm. DAB, 3,3′-diaminobenzidine; anti-Rb, antirabbit immunoglobulin; anti-M, antimouse immunoglobulin; CCs, costal chondrocytes; IHC, immunohistochemical; IF, immunofluorescence.

    Journal: Cell Transplantation

    Article Title: Fully Dedifferentiated Chondrocytes Expanded in Specific Mesenchymal Stem Cell Growth Medium with FGF2 Obtains Mesenchymal Stem Cell Phenotype In Vitro but Retains Chondrocyte Phenotype In Vivo

    doi: 10.1177/0963689717724794

    Figure Lengend Snippet: Characteristics of CCs during in vitro expansion. (A) IHC staining for type I collagen and IF staining for type II collagen, CD29, and α-SMA were performed in CCs cultured in D, M, DF, or MF at passages 2 and 8 ( n = 5). To visualize total cells, nuclei were counterstained with hematoxylin and 4′,6-diamidino-2-phenylindole for IHC and IF, respectively. (B) Negative control experiments confirmed the specificities of the IHC and IF signals. Scale bar, 100 µm. DAB, 3,3′-diaminobenzidine; anti-Rb, antirabbit immunoglobulin; anti-M, antimouse immunoglobulin; CCs, costal chondrocytes; IHC, immunohistochemical; IF, immunofluorescence.

    Article Snippet: For immunohistochemical (IHC) staining, anti-type I collagen antibody (Southern Biotech Associates) was applied to the cover slips at 4 °C overnight, and the results were developed with 0.1% 3,3′-diaminobenzidine tetrahydrochloride (DAB; Vector Laboratories, Burlingame, CA, USA) in PBS for 5 min.

    Techniques: In Vitro, Immunohistochemistry, Staining, Cell Culture, Negative Control, Immunofluorescence

    Distributions of SCP3 in normal and iCAM-cultured mice testes. Serial sections in panels A through C and D through F (high magnification) were stained for SCP3 and visualized with 3,3′-diaminobenzidine tetrahydrochloride (brown). (A and D) Testis sections from iCAM, cultured at 37.5 °C for 12 d. (B) and (E) Testis sections from day 8 mice. (C) and (F) Testis sections from day 19 mice. Magnification, 100× (panels A through C); 400× (scale bar, 10 µm; panels D through F).

    Journal: Comparative Medicine

    Article Title: Use of In Ovo Chorioallantoic Membrane Engraftment to Culture Testes from Neonatal Mice

    doi:

    Figure Lengend Snippet: Distributions of SCP3 in normal and iCAM-cultured mice testes. Serial sections in panels A through C and D through F (high magnification) were stained for SCP3 and visualized with 3,3′-diaminobenzidine tetrahydrochloride (brown). (A and D) Testis sections from iCAM, cultured at 37.5 °C for 12 d. (B) and (E) Testis sections from day 8 mice. (C) and (F) Testis sections from day 19 mice. Magnification, 100× (panels A through C); 400× (scale bar, 10 µm; panels D through F).

    Article Snippet: After the sections were washed with PBS, they were stained with 3,3′-diaminobenzidine tetrahydrochloride (Vector Laboratories) and evaluated.

    Techniques: Cell Culture, Mouse Assay, Staining