3 3 diaminobenzidine tetrahydrochloride (Vector Laboratories)
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Name:
DAB Peroxidase HRP Substrate Kit with Nickel 3 3 diaminobenzidine
Description:
DAB Peroxidase Substrate Kit with Nickel provides greater sensitivity than conventional substrates Consistent and reliableIdeal for IHC ICC ISH and blotsHeat Stable Permanent mountingIdeal for single and multiple labeling Stock solutions supplied in convenient dropper bottles promoting ease of handlingNo wait times for mixing and dissolving powders or tablets Includes nickel to produce a gray black rather than brown reaction product One year expiry dateSufficient reagents to produce 300 ml of working solution DAB 3 3 diaminobenzidine HRP substrate produces a dark brown reaction product and can be used for both immunohistochemical and blotting applications DAB chromogen is effective as a single label or as a second color for multiple antigen labeling Because of its heat resistance DAB can be used in IHC ISH double labeling applications With the aid of imaging systems and software the spectral profile of DAB can be distinguished from our other proprietary enzyme substrates in applications where antigens are co localized Sections stained with DAB can also be viewed by darkfield and electron microscopy Slides developed with DAB can be dehydrated cleared and permanently mounted The DAB reaction product can be intensified with DAB Enhancing Solution H 2200 This kit contains stock solutions in convenient dropper bottles This kit includes nickel providing the option of changing the reaction product from brown to gray black and increasing sensitivity in blot applications Slides developed with DAB Ni should be dehydrated cleared and permanently mounted This product can also be used on blots This kit provides all of the necessary reagents to prepare about 300 ml of working solution When stored at 4C this kit is stable for one year
Catalog Number:
sk-4100
Price:
None
Category:
Proteins
Size:
1 Kit
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Structured Review
Vector Laboratories
3 3 diaminobenzidine tetrahydrochloride
DAB Peroxidase Substrate Kit with Nickel provides greater sensitivity than conventional substrates Consistent and reliableIdeal for IHC ICC ISH and blotsHeat Stable Permanent mountingIdeal for single and multiple labeling Stock solutions supplied in convenient dropper bottles promoting ease of handlingNo wait times for mixing and dissolving powders or tablets Includes nickel to produce a gray black rather than brown reaction product One year expiry dateSufficient reagents to produce 300 ml of working solution DAB 3 3 diaminobenzidine HRP substrate produces a dark brown reaction product and can be used for both immunohistochemical and blotting applications DAB chromogen is effective as a single label or as a second color for multiple antigen labeling Because of its heat resistance DAB can be used in IHC ISH double labeling applications With the aid of imaging systems and software the spectral profile of DAB can be distinguished from our other proprietary enzyme substrates in applications where antigens are co localized Sections stained with DAB can also be viewed by darkfield and electron microscopy Slides developed with DAB can be dehydrated cleared and permanently mounted The DAB reaction product can be intensified with DAB Enhancing Solution H 2200 This kit contains stock solutions in convenient dropper bottles This kit includes nickel providing the option of changing the reaction product from brown to gray black and increasing sensitivity in blot applications Slides developed with DAB Ni should be dehydrated cleared and permanently mounted This product can also be used on blots This kit provides all of the necessary reagents to prepare about 300 ml of working solution When stored at 4C this kit is stable for one year
https://www.bioz.com/result/3 3 diaminobenzidine tetrahydrochloride/product/Vector Laboratories
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
DAB Peroxidase Substrate Kit with Nickel provides greater sensitivity than conventional substrates Consistent and reliableIdeal for IHC ICC ISH and blotsHeat Stable Permanent mountingIdeal for single and multiple labeling Stock solutions supplied in convenient dropper bottles promoting ease of handlingNo wait times for mixing and dissolving powders or tablets Includes nickel to produce a gray black rather than brown reaction product One year expiry dateSufficient reagents to produce 300 ml of working solution DAB 3 3 diaminobenzidine HRP substrate produces a dark brown reaction product and can be used for both immunohistochemical and blotting applications DAB chromogen is effective as a single label or as a second color for multiple antigen labeling Because of its heat resistance DAB can be used in IHC ISH double labeling applications With the aid of imaging systems and software the spectral profile of DAB can be distinguished from our other proprietary enzyme substrates in applications where antigens are co localized Sections stained with DAB can also be viewed by darkfield and electron microscopy Slides developed with DAB can be dehydrated cleared and permanently mounted The DAB reaction product can be intensified with DAB Enhancing Solution H 2200 This kit contains stock solutions in convenient dropper bottles This kit includes nickel providing the option of changing the reaction product from brown to gray black and increasing sensitivity in blot applications Slides developed with DAB Ni should be dehydrated cleared and permanently mounted This product can also be used on blots This kit provides all of the necessary reagents to prepare about 300 ml of working solution When stored at 4C this kit is stable for one year
https://www.bioz.com/result/3 3 diaminobenzidine tetrahydrochloride/product/Vector Laboratories
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
3 3 diaminobenzidine tetrahydrochloride - by Bioz Stars,
2021-03
86/100 stars
Images
1) Product Images from "Up-Regulation of A1M/?1-Microglobulin in Skin by Heme and Reactive Oxygen Species Gives Protection from Oxidative Damage"
Article Title: Up-Regulation of A1M/?1-Microglobulin in Skin by Heme and Reactive Oxygen Species Gives Protection from Oxidative Damage
Journal: PLoS ONE
doi: 10.1371/journal.pone.0027505
Figure Legend Snippet: Histochemical distribution and expression of A1M. A. The sections (x10) were stained for A1M using monoclonal mouse anti-A1M BN11.10 (10 µg/mL) followed by peroxidase/diaminobenzidine tetrahydrochloride incubation as described in Materials and Methods . B . Control sections (x10) incubated without primary antibody. C, D and E. Human skin explants (1×10×2 mm) or primary keratinocytes were cultured in serum-free medium as described in Materials and Methods . C. Skin explants were incubated with 20 µM (NH 4 )Fe(SO 4 ) 2 +200 µM ascorbate+40 µM H 2 O 2 ( = Fenton; white bar) or 20 µM heme (grey) for a period of 20 hours. D. Keratinocytes were incubated with 20 µM heme for a period of 3 (white) or 20 (grey) hours. Total RNA was extracted from homogenized cells, cDNA was prepared using reverse transcription and expression of A1M was analyzed using real-time PCR as described in Materials and Methods . A1M threshold cycle values were normalized against G3DPH and ΔΔCt was calculated by normalizing against control samples which were incubated with buffer only for the same time-periods. Consequently, ΔΔCt-values of all controls are zero and correspond to the baseline. E. Keratinocytes were cultured in serum-free medium as described in Materials and Methods . The cells were then incubated with 20 µM heme for a period of 3 (white) or 20 (grey) hours. The cells were homogenized and the A1M concentration was determined using RIA as described in Materials and Methods . The total protein concentrations were determined with Bradford protein assay as described in Materials and Methods . Results are from triplicate experiments and are presented as mean ± SEM. Statistical comparison with control cultures was made using Students t test. * P
Techniques Used: Expressing, Staining, Incubation, Cell Culture, Real-time Polymerase Chain Reaction, Concentration Assay, Bradford Protein Assay
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