2x premixed hotstart taq polymerase  (Qiagen)


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    Structured Review

    Qiagen 2x premixed hotstart taq polymerase
    2x Premixed Hotstart Taq Polymerase, supplied by Qiagen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/2x premixed hotstart taq polymerase/product/Qiagen
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    2x premixed hotstart taq polymerase - by Bioz Stars, 2020-04
    86/100 stars

    Related Products / Commonly Used Together

    anchor primer ap1
    cdna
    double-stranded pcr

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    Related Articles

    Clone Assay:

    Article Title: A member of the cathelicidin family of antimicrobial peptides is produced in the upper airway of the chinchilla and its mRNA expression is altered by common viral and bacterial co-pathogens of otitis media
    Article Snippet: Paragraph title: 2.2 Cloning of cCRAMP cDNA ... The resulting cDNA was amplified to make a double-stranded PCR product using 2X premixed HotStart Taq polymerase (Qiagen, Valencia, CA), the Clontech anchor primer AP1 and primer CATH2 ( ).

    Amplification:

    Article Title: A member of the cathelicidin family of antimicrobial peptides is produced in the upper airway of the chinchilla and its mRNA expression is altered by common viral and bacterial co-pathogens of otitis media
    Article Snippet: .. The resulting cDNA was amplified to make a double-stranded PCR product using 2X premixed HotStart Taq polymerase (Qiagen, Valencia, CA), the Clontech anchor primer AP1 and primer CATH2 ( ). .. The sequence of CATH2 was based on conserved regions in the cathelin domain of human CAP-18 and rat CRAMP.

    Agarose Gel Electrophoresis:

    Article Title: A member of the cathelicidin family of antimicrobial peptides is produced in the upper airway of the chinchilla and its mRNA expression is altered by common viral and bacterial co-pathogens of otitis media
    Article Snippet: The resulting cDNA was amplified to make a double-stranded PCR product using 2X premixed HotStart Taq polymerase (Qiagen, Valencia, CA), the Clontech anchor primer AP1 and primer CATH2 ( ). .. The cDNA product was size-fractionated in an agarose gel and a band of approximately 450 bp was extracted using the Qiaquick PCR purification kit (Qiagen).

    Polymerase Chain Reaction:

    Article Title: A member of the cathelicidin family of antimicrobial peptides is produced in the upper airway of the chinchilla and its mRNA expression is altered by common viral and bacterial co-pathogens of otitis media
    Article Snippet: .. The resulting cDNA was amplified to make a double-stranded PCR product using 2X premixed HotStart Taq polymerase (Qiagen, Valencia, CA), the Clontech anchor primer AP1 and primer CATH2 ( ). .. The sequence of CATH2 was based on conserved regions in the cathelin domain of human CAP-18 and rat CRAMP.

    Isolation:

    Article Title: A member of the cathelicidin family of antimicrobial peptides is produced in the upper airway of the chinchilla and its mRNA expression is altered by common viral and bacterial co-pathogens of otitis media
    Article Snippet: Total RNA was isolated from the Eustachian tube and nasopharyngeal mucosae of a chinchilla and a first-strand cDNA product was amplified using a modified oligo(dT) primer ( ) (Marathon cDNA synthesis primer, Clontech, Palo Alto, CA), according to the manufacturer’s suggestions. .. The resulting cDNA was amplified to make a double-stranded PCR product using 2X premixed HotStart Taq polymerase (Qiagen, Valencia, CA), the Clontech anchor primer AP1 and primer CATH2 ( ).

    Purification:

    Article Title: A member of the cathelicidin family of antimicrobial peptides is produced in the upper airway of the chinchilla and its mRNA expression is altered by common viral and bacterial co-pathogens of otitis media
    Article Snippet: The resulting cDNA was amplified to make a double-stranded PCR product using 2X premixed HotStart Taq polymerase (Qiagen, Valencia, CA), the Clontech anchor primer AP1 and primer CATH2 ( ). .. The cDNA product was size-fractionated in an agarose gel and a band of approximately 450 bp was extracted using the Qiaquick PCR purification kit (Qiagen).

    Sequencing:

    Article Title: A member of the cathelicidin family of antimicrobial peptides is produced in the upper airway of the chinchilla and its mRNA expression is altered by common viral and bacterial co-pathogens of otitis media
    Article Snippet: The resulting cDNA was amplified to make a double-stranded PCR product using 2X premixed HotStart Taq polymerase (Qiagen, Valencia, CA), the Clontech anchor primer AP1 and primer CATH2 ( ). .. The sequence of CATH2 was based on conserved regions in the cathelin domain of human CAP-18 and rat CRAMP.

    Modification:

    Article Title: A member of the cathelicidin family of antimicrobial peptides is produced in the upper airway of the chinchilla and its mRNA expression is altered by common viral and bacterial co-pathogens of otitis media
    Article Snippet: Total RNA was isolated from the Eustachian tube and nasopharyngeal mucosae of a chinchilla and a first-strand cDNA product was amplified using a modified oligo(dT) primer ( ) (Marathon cDNA synthesis primer, Clontech, Palo Alto, CA), according to the manufacturer’s suggestions. .. The resulting cDNA was amplified to make a double-stranded PCR product using 2X premixed HotStart Taq polymerase (Qiagen, Valencia, CA), the Clontech anchor primer AP1 and primer CATH2 ( ).

    Transformation Assay:

    Article Title: A member of the cathelicidin family of antimicrobial peptides is produced in the upper airway of the chinchilla and its mRNA expression is altered by common viral and bacterial co-pathogens of otitis media
    Article Snippet: The resulting cDNA was amplified to make a double-stranded PCR product using 2X premixed HotStart Taq polymerase (Qiagen, Valencia, CA), the Clontech anchor primer AP1 and primer CATH2 ( ). .. This amplicon was cloned into pGEM-T ( ; Promega, Madison, WI) via annealing of terminal 3′-thymidine overhangs on the vector to non-template deoxyadenosine bases on the amplified product and was transformed into E. coli ) and a single plasmid containing the cCRAMP insert was saved as pcCRAMP1 ( ).

    Plasmid Preparation:

    Article Title: A member of the cathelicidin family of antimicrobial peptides is produced in the upper airway of the chinchilla and its mRNA expression is altered by common viral and bacterial co-pathogens of otitis media
    Article Snippet: The resulting cDNA was amplified to make a double-stranded PCR product using 2X premixed HotStart Taq polymerase (Qiagen, Valencia, CA), the Clontech anchor primer AP1 and primer CATH2 ( ). .. This amplicon was cloned into pGEM-T ( ; Promega, Madison, WI) via annealing of terminal 3′-thymidine overhangs on the vector to non-template deoxyadenosine bases on the amplified product and was transformed into E. coli ) and a single plasmid containing the cCRAMP insert was saved as pcCRAMP1 ( ).

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  • News
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  • Contact
  • Bioz Stars
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  • 86
    Qiagen 2x premixed hotstart taq polymerase
    2x Premixed Hotstart Taq Polymerase, supplied by Qiagen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/2x premixed hotstart taq polymerase/product/Qiagen
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    2x premixed hotstart taq polymerase - by Bioz Stars, 2020-04
    86/100 stars
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