2x hotstar taq polymerase  (Qiagen)


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    Structured Review

    Qiagen 2x hotstar taq polymerase
    2x Hotstar Taq Polymerase, supplied by Qiagen, used in various techniques. Bioz Stars score: 87/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/2x hotstar taq polymerase/product/Qiagen
    Average 87 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    2x hotstar taq polymerase - by Bioz Stars, 2020-04
    87/100 stars

    Related Products / Commonly Used Together

    pcr
    primer 's anti-sense
    unlabeled primer

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    Related Articles

    Amplification:

    Article Title: A Combined Enrichment and Aptamer Pulldown Assay for Francisella tularensis Detection in Food and Environmental Matrices
    Article Snippet: PCR was performed using a biotin-labeled on the primer’s anti-sense strand (WP20R1; 5 pmol), an unlabeled primer (WP20F1; 5 pmol), 2X Hotstar taq polymerase (Qiagen, Valencia, CA) and the following program: 95°C for 15 min, followed by 15 cycles of 95°C for 30 s, 63°C for 30 s, and 72°C for 7 min ( ). .. The amplicon sense strand was applied to the remaining rounds of SELEX.

    Polymerase Chain Reaction:

    Article Title: A Combined Enrichment and Aptamer Pulldown Assay for Francisella tularensis Detection in Food and Environmental Matrices
    Article Snippet: .. PCR was performed using a biotin-labeled on the primer’s anti-sense strand (WP20R1; 5 pmol), an unlabeled primer (WP20F1; 5 pmol), 2X Hotstar taq polymerase (Qiagen, Valencia, CA) and the following program: 95°C for 15 min, followed by 15 cycles of 95°C for 30 s, 63°C for 30 s, and 72°C for 7 min ( ). .. In order to enrich for aptamer sense strands needed for further rounds of SELEX, amplicons were applied as templates at 1∶10 and 1∶25 dilutions for asymmetric-touchdown PCR using 30 pmol of WP20F1 and 1.2 pmol of biotin labeled WP20R1.

    Cell Culture:

    Article Title: A Combined Enrichment and Aptamer Pulldown Assay for Francisella tularensis Detection in Food and Environmental Matrices
    Article Snippet: The aptamer library was incubated for 30 min with 108 CFU/mL of soil bacteria (previously cultured overnight at both 25°C or 37°C) in TSB containing 0.1% L-cysteine at room temperature with gentle shaking (Labquake tube shaker) , . .. PCR was performed using a biotin-labeled on the primer’s anti-sense strand (WP20R1; 5 pmol), an unlabeled primer (WP20F1; 5 pmol), 2X Hotstar taq polymerase (Qiagen, Valencia, CA) and the following program: 95°C for 15 min, followed by 15 cycles of 95°C for 30 s, 63°C for 30 s, and 72°C for 7 min ( ).

    Modification:

    Article Title: A Combined Enrichment and Aptamer Pulldown Assay for Francisella tularensis Detection in Food and Environmental Matrices
    Article Snippet: Briefly, a modified single-stranded aptamer library (WAP40m) consisting of a 40-mer randomized regions flanked by constant-primer binding regions (Integrated DNA Technologies, Inc., Coralville, IA) underwent four rounds of counter-SELEX iterations. .. PCR was performed using a biotin-labeled on the primer’s anti-sense strand (WP20R1; 5 pmol), an unlabeled primer (WP20F1; 5 pmol), 2X Hotstar taq polymerase (Qiagen, Valencia, CA) and the following program: 95°C for 15 min, followed by 15 cycles of 95°C for 30 s, 63°C for 30 s, and 72°C for 7 min ( ).

    Incubation:

    Article Title: A Combined Enrichment and Aptamer Pulldown Assay for Francisella tularensis Detection in Food and Environmental Matrices
    Article Snippet: The aptamer library was incubated for 30 min with 108 CFU/mL of soil bacteria (previously cultured overnight at both 25°C or 37°C) in TSB containing 0.1% L-cysteine at room temperature with gentle shaking (Labquake tube shaker) , . .. PCR was performed using a biotin-labeled on the primer’s anti-sense strand (WP20R1; 5 pmol), an unlabeled primer (WP20F1; 5 pmol), 2X Hotstar taq polymerase (Qiagen, Valencia, CA) and the following program: 95°C for 15 min, followed by 15 cycles of 95°C for 30 s, 63°C for 30 s, and 72°C for 7 min ( ).

    Selection:

    Article Title: A Combined Enrichment and Aptamer Pulldown Assay for Francisella tularensis Detection in Food and Environmental Matrices
    Article Snippet: Paragraph title: Selection of DNA aptamers against F. tularensis using Systemic Evolution of Ligands by Exponential Enrichment (SELEX) ... PCR was performed using a biotin-labeled on the primer’s anti-sense strand (WP20R1; 5 pmol), an unlabeled primer (WP20F1; 5 pmol), 2X Hotstar taq polymerase (Qiagen, Valencia, CA) and the following program: 95°C for 15 min, followed by 15 cycles of 95°C for 30 s, 63°C for 30 s, and 72°C for 7 min ( ).

    Labeling:

    Article Title: A Combined Enrichment and Aptamer Pulldown Assay for Francisella tularensis Detection in Food and Environmental Matrices
    Article Snippet: PCR was performed using a biotin-labeled on the primer’s anti-sense strand (WP20R1; 5 pmol), an unlabeled primer (WP20F1; 5 pmol), 2X Hotstar taq polymerase (Qiagen, Valencia, CA) and the following program: 95°C for 15 min, followed by 15 cycles of 95°C for 30 s, 63°C for 30 s, and 72°C for 7 min ( ). .. In order to enrich for aptamer sense strands needed for further rounds of SELEX, amplicons were applied as templates at 1∶10 and 1∶25 dilutions for asymmetric-touchdown PCR using 30 pmol of WP20F1 and 1.2 pmol of biotin labeled WP20R1.

    IA:

    Article Title: A Combined Enrichment and Aptamer Pulldown Assay for Francisella tularensis Detection in Food and Environmental Matrices
    Article Snippet: Briefly, a modified single-stranded aptamer library (WAP40m) consisting of a 40-mer randomized regions flanked by constant-primer binding regions (Integrated DNA Technologies, Inc., Coralville, IA) underwent four rounds of counter-SELEX iterations. .. PCR was performed using a biotin-labeled on the primer’s anti-sense strand (WP20R1; 5 pmol), an unlabeled primer (WP20F1; 5 pmol), 2X Hotstar taq polymerase (Qiagen, Valencia, CA) and the following program: 95°C for 15 min, followed by 15 cycles of 95°C for 30 s, 63°C for 30 s, and 72°C for 7 min ( ).

    Binding Assay:

    Article Title: A Combined Enrichment and Aptamer Pulldown Assay for Francisella tularensis Detection in Food and Environmental Matrices
    Article Snippet: Briefly, a modified single-stranded aptamer library (WAP40m) consisting of a 40-mer randomized regions flanked by constant-primer binding regions (Integrated DNA Technologies, Inc., Coralville, IA) underwent four rounds of counter-SELEX iterations. .. PCR was performed using a biotin-labeled on the primer’s anti-sense strand (WP20R1; 5 pmol), an unlabeled primer (WP20F1; 5 pmol), 2X Hotstar taq polymerase (Qiagen, Valencia, CA) and the following program: 95°C for 15 min, followed by 15 cycles of 95°C for 30 s, 63°C for 30 s, and 72°C for 7 min ( ).

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