2sb 3ct (Millipore)

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Name:

SB 3CT

Description:

Catalog Number:

s1326

Price:

None

Applications:

SB−3CT has been used as irreversible inhibitor of matrix metallopeptidase 9 (MMP9) in cell cultures to exclude reciprocal regulation.

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Structured Review

Millipore 2sb 3ct
SB 3CT

https://www.bioz.com/result/2sb 3ct/product/Millipore
Average 96 stars, based on 1 article reviews
Price from $9.99 to $1999.99

2sb 3ct - by Bioz Stars, 2020-09

96/100 stars

Images

Immunohistochemistry:

Article Title: Tissue inhibitor of metalloproteinases 1 enhances rod survival in the rd1 mouse retina
Article Snippet: .. Thus, in the current study, we injected either saline, SB-3CT or TIMP1 to P15 rd1 mice, and then performed rhodopsin immunohistochemical staining on P30, P35, and P45 whole-mount retinas for quantitative analysis to examine the rod cell survival in the rd1 retina. .. For each animal, the left eye was injected with TIMP1 or SB-3CT, and the right eye was injected with saline for comparison. shows the mean rod density measured from the 0.25 mm2 sampling area (for detail, see ) of saline-, SB-3CT-, and TIMP1-treated rd1 retinas.

Cell Culture:

Article Title: Matrix metalloproteinase-9 (MMP9) is involved in the TNF-α-induced fusion of human M13SV1-Cre breast epithelial cells and human MDA-MB-435-pFDR1 cancer cells
Article Snippet: .. Depending on the experiment 100 ng/ml TNF-α (Bio-Techne GmbH, Wiesbaden-Nordenstadt, Germany), up to 10 μg/ml minocycline (Sigma-Aldrich, Taufkirchen, Germany), up to 10 μM SB-3CT (Sigma Aldrich, Taufkirchen, Germany), 10 μg/ml anti-ICAM1/CD54 (mouse monoclonal, clone 11C81; Bio-Techne GmbH, Wiesbaden-Nordenstadt, Germany) and appropriate combinations of inhibitors and TNF-α were added to the cell culture media. .. Hypoxia was induced by culturing the cells in stepwise oxygen-deprivation conditions (24 h with 10% O2 , 24 h with 5% O2 , 24 h with 1% O2 ) in hypoxia chambers (Billups-Rothenberg, Del Mar, CA, USA) with a constant flow of 5% CO2 and the remainder of nitrogen, as previously described [ ].

Mouse Assay:

Inhibition:

Article Title: Inhibition of Matrix Metalloproteinase 9 Enhances Rod Survival in the S334ter-line3 Retinitis Pigmentosa Model
Article Snippet: .. SB-3CT treatment delays rod death in S334ter retina To determine if inhibition of up-regulated MMP-9 via SB-3CT (Figs and ) can affect cell survival, we injected either SB-3CT (25 ug/ml) or saline (for control) to P15 S334ter rats. .. After a single injection at P15, animals were sacrificed at P30, P45 and P60.

Staining:

Injection:

Article Title: Tissue inhibitor of metalloproteinases 1 enhances rod survival in the rd1 mouse retina
Article Snippet: .. To explore these alternative independent pathways, we injected saline, SB-3CT or TIMP1 to P15 rd1 eyes and performed immunoblot analysis on the retinas. ..

Article Title: Tissue inhibitor of metalloproteinases 1 enhances rod survival in the rd1 mouse retina
Article Snippet: .. The developmental stage for the injection of SB-3CT or TIMP1 was either P15 (i.e. time period of peak rod death and time of eye opening) [ ], or P45, when the majority of rod photoreceptor cells were degenerated (see ). .. Surgeries on mice were performed under anesthesia induced by intraperitoneal injection of ketamine (20 mg/kg; KETASET, Fort Dodge, IA, USA) and xylazine (5 mg/kg, X-Ject SA; Butler, Dublin, OH, USA).

other:

Article Title: Inhibition of Matrix Metalloproteinase 9 Enhances Rod Survival in the S334ter-line3 Retinitis Pigmentosa Model
Article Snippet: First, the safety of SB-3CT in concentrations used for intravitreal injections (10, 25 and 50 ug/ml) was tested at P30.

Article Title: Induction of MMP-9 Expression and Endothelial Injury by Oxidative Stress after Spinal Cord Injury
Article Snippet: BBB disruption was evaluated in the Wt and SOD1 Tg rats, as well as in the SB-3CT– and DMSO-treated rats.

Article Title: Effect of ablation or inhibition of stromal matrix metalloproteinase-9 on lung metastasis in a breast cancer model is dependent on genetic background
Article Snippet: The gelatinase-selective inhibitor SB-3CT [(4-phenoxyphenylsulfonyl) methylthiirane] [ ] was stored as a powder at -20°C, and working solution was prepared fresh daily by dissolving in 1/10th volume of dimethylsulfoxide (DMSO; Sigma) and addition of water with constant stirring.