2mercaptoethanol  (Thermo Fisher)


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    Name:
    Gibco 2 Mercaptoethanol
    Description:
    Gibco 2 Mercaptoethanol also known as beta mercaptoethanol or BME is a potent reducing agent used in cell culture media to prevent toxic levels of oxygen radicals 2 Mercaptoethanol is not stable in solution so most protocols require daily supplementation Gibco 2 Mercaptoethanol contains 2 mercaptoethanol at a concentration of 55 mM in Dulbecco s phosphate buffered saline DPBS cGMP manufacturing and quality systemGibco Reducing Agent is manufactured at a cGMP compliant facility located in Grand Island New York The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards
    Catalog Number:
    21985023
    Price:
    None
    Applications:
    Cell Culture|Mammalian Cell Culture
    Category:
    Cell Culture Transfection Reagents
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    Structured Review

    Thermo Fisher 2mercaptoethanol
    Gibco 2 Mercaptoethanol also known as beta mercaptoethanol or BME is a potent reducing agent used in cell culture media to prevent toxic levels of oxygen radicals 2 Mercaptoethanol is not stable in solution so most protocols require daily supplementation Gibco 2 Mercaptoethanol contains 2 mercaptoethanol at a concentration of 55 mM in Dulbecco s phosphate buffered saline DPBS cGMP manufacturing and quality systemGibco Reducing Agent is manufactured at a cGMP compliant facility located in Grand Island New York The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards
    https://www.bioz.com/result/2mercaptoethanol/product/Thermo Fisher
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    2mercaptoethanol - by Bioz Stars, 2020-07
    99/100 stars

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    Related Articles

    Isolation:

    Article Title: Cystathionine Is a Novel Substrate of Cystine/Glutamate Transporter
    Article Snippet: .. Mouse embryonic fibroblasts (MEF) isolated from wild-type and xCT-deficient mice ( ) and xCT-overexpressing MEF ( ) were cultured routinely in Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum, 2-mercaptoethanol (50 μ m ), insulin-transferrin-selenium A (Life Technologies, Inc.), penicillin (50 units/ml), and streptomycin (50 μg/ml) at 37 °C in 5% CO2 and 95% air. .. The addition of 2-mercaptoethanol is necessary for xCT-deficient MEF to grow even under routine cell culture conditions ( , ).

    Knock-Out:

    Article Title: Efficient Generation of Hepatoblasts From Human ES Cells and iPS Cells by Transient Overexpression of Homeobox Gene HEX
    Article Snippet: .. Human ESCs were maintained on a feeder layer of mitomycin-inactivated mouse embryonic fibroblasts (ICR; ReproCELL Incorporated, Tokyo, Japan) with Dulbecco's modified Eagle's medium/F-12 (Sigma, St Louis, MO) supplemented with 0.1 mmol/l 2-mercaptoethanol, 0.1 mmol/l nonessential amino acids, 2 mmol/l -glutamine, 20% GIBCO knockout serum replacement (Invitrogen), and 5 ng/ml bFGF (Sigma) in a humidified atmosphere of 3% CO2 and 97% air at 37 °C. .. Human ESCs were dissociated with 0.1 mg/ml dispase (Roche Diagnostics, Burgess Hill, UK) into small clumps, and subcultured every 5 or 6 days.

    Mouse Assay:

    Article Title: Cystathionine Is a Novel Substrate of Cystine/Glutamate Transporter
    Article Snippet: .. Mouse embryonic fibroblasts (MEF) isolated from wild-type and xCT-deficient mice ( ) and xCT-overexpressing MEF ( ) were cultured routinely in Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum, 2-mercaptoethanol (50 μ m ), insulin-transferrin-selenium A (Life Technologies, Inc.), penicillin (50 units/ml), and streptomycin (50 μg/ml) at 37 °C in 5% CO2 and 95% air. .. The addition of 2-mercaptoethanol is necessary for xCT-deficient MEF to grow even under routine cell culture conditions ( , ).

    Modification:

    Article Title: Efficient Generation of Hepatoblasts From Human ES Cells and iPS Cells by Transient Overexpression of Homeobox Gene HEX
    Article Snippet: .. Human ESCs were maintained on a feeder layer of mitomycin-inactivated mouse embryonic fibroblasts (ICR; ReproCELL Incorporated, Tokyo, Japan) with Dulbecco's modified Eagle's medium/F-12 (Sigma, St Louis, MO) supplemented with 0.1 mmol/l 2-mercaptoethanol, 0.1 mmol/l nonessential amino acids, 2 mmol/l -glutamine, 20% GIBCO knockout serum replacement (Invitrogen), and 5 ng/ml bFGF (Sigma) in a humidified atmosphere of 3% CO2 and 97% air at 37 °C. .. Human ESCs were dissociated with 0.1 mg/ml dispase (Roche Diagnostics, Burgess Hill, UK) into small clumps, and subcultured every 5 or 6 days.

    Article Title: Cystathionine Is a Novel Substrate of Cystine/Glutamate Transporter
    Article Snippet: .. Mouse embryonic fibroblasts (MEF) isolated from wild-type and xCT-deficient mice ( ) and xCT-overexpressing MEF ( ) were cultured routinely in Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum, 2-mercaptoethanol (50 μ m ), insulin-transferrin-selenium A (Life Technologies, Inc.), penicillin (50 units/ml), and streptomycin (50 μg/ml) at 37 °C in 5% CO2 and 95% air. .. The addition of 2-mercaptoethanol is necessary for xCT-deficient MEF to grow even under routine cell culture conditions ( , ).

    Cell Culture:

    Article Title: Potential of Adipose-Derived Mesenchymal Stem Cells and Skeletal Muscle-Derived Satellite Cells for Somatic Cell Nuclear Transfer Mediated Transgenesis in Arbas Cashmere Goats
    Article Snippet: .. Neurogenic Differentiation and Identification For neurogenic induction, 70% confluent gADSCs and gMDSCs were cultured in DMEM/F12 medium supplemented with 10 ng/mL epidermal growth factor (G5021; Promega, Madison, WI) plus 10 ng/mL basic fibroblast growth factor (bFGF) (G5071, Promega, Madison, WI) for 24 h. Subsequently, the medium was changed to DMEM/F12 supplemented with 1 mM β-mercaptoethanol (21985-023, Gibco, Gaithersburg, MD) and 10% FBS for 6 h for induction. .. The cells were then washed three times with PBS to remove β-mercaptoethanol and cultured in DMEM/F12 supplemented with 2% DMSO, 200 μM butylated hydroxyanisole (20021; Sigma–Aldrich, St. Louis, MO) and 20 ng/mL bFGF.

    Article Title: Cystathionine Is a Novel Substrate of Cystine/Glutamate Transporter
    Article Snippet: .. Mouse embryonic fibroblasts (MEF) isolated from wild-type and xCT-deficient mice ( ) and xCT-overexpressing MEF ( ) were cultured routinely in Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum, 2-mercaptoethanol (50 μ m ), insulin-transferrin-selenium A (Life Technologies, Inc.), penicillin (50 units/ml), and streptomycin (50 μg/ml) at 37 °C in 5% CO2 and 95% air. .. The addition of 2-mercaptoethanol is necessary for xCT-deficient MEF to grow even under routine cell culture conditions ( , ).

    Ex Vivo:

    Article Title: EPO receptor circuits for primary erythroblast survival
    Article Snippet: .. Red cells then were lysed, and progenitors were collected through 50% FBS in PBS., For ex vivo expansions, cells were plated at 8 × 105 cells/mL (7 mL per 100-mm dish) in StemPro-34 (Invitrogen) supplemented with 2.5 U/mL EPO, 100 ng/mL mSCF, 1 μM dexamethasone, 1 μM beta-estradiol, 75 μg/mL h-transferrin, (Sigma-Aldrich, St Louis, MO), 0.5% BSA (StemCell Technologies, Vancouver, BC), 0.1 mM 2-mercaptoethanol, 100 U/mL penicillin G, 100 μg/mL streptomycin, 0.25 μg/mL amphotericin B (1XPSF) (Invitrogen), and 1.5 mM l -glutamine (ie, “SP34-EX” medium). .. In the isolation of Kitpos CD71high Ter119neg erythroblasts, Linpos cells first were depleted from ex vivo cultures using biotinylated antibodies to CD5 (Ly-1), CD45R/B220, CDllb (Mac1), Ter119, and Ly6G (Gr1; StemCell Technologies).

    Lysis:

    Article Title: Discovery and Characterization of the First Archaeal Dihydromethanopterin Reductase, an Iron-Sulfur Flavoprotein from Methanosarcina mazei
    Article Snippet: .. About 5 g of cells was suspended with 10 ml of lysis buffer A (50 mM sodium phosphate [pH 8.0], 300 mM sodium chloride, 15 mM 2-mercaptoethanol, 20 mM imidazole) and broken with a Thermo Scientific French press (Fisher Scientific) at 20,000 lb/in2 . .. The mixture was centrifuged with a Sorvall F18S rotor at 27,000 × g for 30 min at 4°C, and the supernatant was filtered with 0.45-μm syringe filters (Millipore, Billerica, MA).