293fectin  (Thermo Fisher)


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    Name:
    293fectin Transfection Reagent
    Description:
    293fectin Transfection Reagent is a proprietary cationic lipid based formulation for transfecting DNA into eukaryotic cells This reagent is optimized for transfecting suspension 293 human embryonic kidney cells e g FreeStyle 293 F cells Cat No R790 07 in defined serum free FreeStyle 293 Expression Medium Cat No 12338 018 and is intended for use with the FreeStyle 293 Expression System Cat No K9000 01 293fectin Transfection Reagent provides the following advantages • Demonstrates high transfection efficiency in suspension 293 cells and is also suitable for transfecting adherent 293 cells• Suspension FreeStyle 293 F cells may be transfected in FreeStyle 293 Expression Medium no medium change is required• Add 293fectin reagent complexes directly to cells in culture medium• No need to remove complexes or change or add medium following transfection
    Catalog Number:
    12347019
    Price:
    None
    Applications:
    Bioproduction|Cell Culture|Mammalian Expression|Mammalian Protein Production|Plasmid Transfection|Protein Biology|Protein Expression|Transient Protein Production & High-Throughput Screening|Antibody & Cell Culture Production|Transfection
    Category:
    Cell Culture Transfection Reagents
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    Structured Review

    Thermo Fisher 293fectin
    293fectin Transfection Reagent is a proprietary cationic lipid based formulation for transfecting DNA into eukaryotic cells This reagent is optimized for transfecting suspension 293 human embryonic kidney cells e g FreeStyle 293 F cells Cat No R790 07 in defined serum free FreeStyle 293 Expression Medium Cat No 12338 018 and is intended for use with the FreeStyle 293 Expression System Cat No K9000 01 293fectin Transfection Reagent provides the following advantages • Demonstrates high transfection efficiency in suspension 293 cells and is also suitable for transfecting adherent 293 cells• Suspension FreeStyle 293 F cells may be transfected in FreeStyle 293 Expression Medium no medium change is required• Add 293fectin reagent complexes directly to cells in culture medium• No need to remove complexes or change or add medium following transfection
    https://www.bioz.com/result/293fectin/product/Thermo Fisher
    Average 99 stars, based on 258 article reviews
    Price from $9.99 to $1999.99
    293fectin - by Bioz Stars, 2020-08
    99/100 stars

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    Related Articles

    Transfection:

    Article Title: A cysteine-based molecular code informs collagen C-propeptide assembly
    Article Snippet: .. Collagen C-Pro expression and purification HEK293 Freestyle cells (Thermo Fisher Scientific) were transfected at a cell density of 1.0 × 106 cells/mL in 80 mL of Freestyle media with 53 μg of appropriate plasmids using 293Fectin (Life Technologies) at a 2:1 ratio of 293Fectin:DNA. .. Cells were split 1:2 3 days post-transfection and the supernatant was harvested 6 days post-transfection after pelleting the cells by centrifugation.

    Article Title: Conformational Plasticity in the HIV-1 Fusion Peptide Facilitates Recognition by Broadly Neutralizing Antibodies
    Article Snippet: .. Briefly, cells were co-transfected with SOSIP and furin plasmids using a ratio of 4:1, and 293Fectin (Invitrogen) as the transfection reagent. ..

    Article Title: Epitope determines efficacy of therapeutic anti-Tau antibodies in a functional assay with human Alzheimer Tau
    Article Snippet: .. Preparation of HEK293F cells expressing human P301S Tau HEK293F cells (Thermo Fisher Scientific) were transiently transfected with the pcDNA3.1(+) vector expressing human Tau 2N4R with a P301S mutation, using 293Fectin (Thermo Fisher Scientific) according to the manufacturer’s instructions. .. Cell seeding For western blot analysis, HEK293F cells expressing untagged human 2N4R P301S Tau were plated on 6-well dishes at a density of 1,000,000 cells per well in complete 293 expression medium (Thermo Fisher Scientific) containing 10% fetal bovine serum and 1% Penicillin–Streptomycin.

    Article Title: Targeted Isolation of Antibodies Directed against Major Sites of SIV Env Vulnerability
    Article Snippet: .. Protein production and purification All SIV proteins and CD4-Ig were expressed by transient transfection of 293Freestyle (293F) cells in serum-free medium using 293fectin transfection reagent (Invitrogen) according to manufacturer’s instructions. .. Cell culture supernatants were harvested 6 days post-transfection, passed through a 0.22 μm filter to remove any cell debris and supplemented with protease inhibitor tablets (Roche).

    Article Title: PROTEIN THERAPEUTICS FOR JUNCTIONAL EPIDERMOLYSIS BULLOSA: INCORPORATION OF RECOMBINANT ?3 CHAIN INTO LAMININ 332 IN ?3-/- KERATINOCYTES IN VITRO
    Article Snippet: .. Optimization of DNA transfection into 293F cells was carried out using 293Fectin™ transfection reagent (Invitrogen, Carlsbad, CA) and Amaxa nucleofection reaction (Amaxa Inc, Köln, Germany). .. Two and 4 μg of the pMaxGFP plasmid encoding green fluorescent protein (Amaxa Inc, Köln, Germany) were used for transfection and nucleofection, respectively.

    Article Title: PLAP efficiently generates mature antigenic peptides in vitro but in patterns distinct from ERAP1 1
    Article Snippet: .. Transfection was performed using the 293Fectin transfection reagent according to the freestyle293™ expression system (Invitrogen) instructions. .. Three days post tranfection the cell supernatant was harvested and expression confirmed by L-AMC assay and by western blotting using an anti-5xHis-HRP conjugate antibody (Qiagen).

    Article Title: Structural Insights into Neonatal Fc Receptor-based Recycling Mechanisms
    Article Snippet: .. HEK 293 cells were transiently transfected with the various HSA constructs using 293Fectin (Invitrogen) and standard protocols. ..

    Mutagenesis:

    Article Title: Epitope determines efficacy of therapeutic anti-Tau antibodies in a functional assay with human Alzheimer Tau
    Article Snippet: .. Preparation of HEK293F cells expressing human P301S Tau HEK293F cells (Thermo Fisher Scientific) were transiently transfected with the pcDNA3.1(+) vector expressing human Tau 2N4R with a P301S mutation, using 293Fectin (Thermo Fisher Scientific) according to the manufacturer’s instructions. .. Cell seeding For western blot analysis, HEK293F cells expressing untagged human 2N4R P301S Tau were plated on 6-well dishes at a density of 1,000,000 cells per well in complete 293 expression medium (Thermo Fisher Scientific) containing 10% fetal bovine serum and 1% Penicillin–Streptomycin.

    Construct:

    Article Title: Structural Insights into Neonatal Fc Receptor-based Recycling Mechanisms
    Article Snippet: .. HEK 293 cells were transiently transfected with the various HSA constructs using 293Fectin (Invitrogen) and standard protocols. ..

    Purification:

    Article Title: A cysteine-based molecular code informs collagen C-propeptide assembly
    Article Snippet: .. Collagen C-Pro expression and purification HEK293 Freestyle cells (Thermo Fisher Scientific) were transfected at a cell density of 1.0 × 106 cells/mL in 80 mL of Freestyle media with 53 μg of appropriate plasmids using 293Fectin (Life Technologies) at a 2:1 ratio of 293Fectin:DNA. .. Cells were split 1:2 3 days post-transfection and the supernatant was harvested 6 days post-transfection after pelleting the cells by centrifugation.

    Article Title: Targeted Isolation of Antibodies Directed against Major Sites of SIV Env Vulnerability
    Article Snippet: .. Protein production and purification All SIV proteins and CD4-Ig were expressed by transient transfection of 293Freestyle (293F) cells in serum-free medium using 293fectin transfection reagent (Invitrogen) according to manufacturer’s instructions. .. Cell culture supernatants were harvested 6 days post-transfection, passed through a 0.22 μm filter to remove any cell debris and supplemented with protease inhibitor tablets (Roche).

    Cell Culture:

    Article Title: Differential processing of HIV envelope glycans on the virus and soluble recombinant trimer
    Article Snippet: .. Membrane-bound Env trimer expression JR-FL ∆CT, BG505 ∆CT N332, BG505 ∆CT T332, and B41 ∆CT expression plasmids were transiently co-transfected with furin plasmids in a 2:1 ratio into 293F cells (Invitrogen), using 293Fectin (Invitrogen), cultured in 293 Freestyle media (Life Technologies) and grown for 72 h at 37 °C. .. Membrane-bound Env trimers were then isolated using PGT151-TEV pull down.

    Expressing:

    Article Title: A cysteine-based molecular code informs collagen C-propeptide assembly
    Article Snippet: .. Collagen C-Pro expression and purification HEK293 Freestyle cells (Thermo Fisher Scientific) were transfected at a cell density of 1.0 × 106 cells/mL in 80 mL of Freestyle media with 53 μg of appropriate plasmids using 293Fectin (Life Technologies) at a 2:1 ratio of 293Fectin:DNA. .. Cells were split 1:2 3 days post-transfection and the supernatant was harvested 6 days post-transfection after pelleting the cells by centrifugation.

    Article Title: Epitope determines efficacy of therapeutic anti-Tau antibodies in a functional assay with human Alzheimer Tau
    Article Snippet: .. Preparation of HEK293F cells expressing human P301S Tau HEK293F cells (Thermo Fisher Scientific) were transiently transfected with the pcDNA3.1(+) vector expressing human Tau 2N4R with a P301S mutation, using 293Fectin (Thermo Fisher Scientific) according to the manufacturer’s instructions. .. Cell seeding For western blot analysis, HEK293F cells expressing untagged human 2N4R P301S Tau were plated on 6-well dishes at a density of 1,000,000 cells per well in complete 293 expression medium (Thermo Fisher Scientific) containing 10% fetal bovine serum and 1% Penicillin–Streptomycin.

    Article Title: Differential processing of HIV envelope glycans on the virus and soluble recombinant trimer
    Article Snippet: .. Membrane-bound Env trimer expression JR-FL ∆CT, BG505 ∆CT N332, BG505 ∆CT T332, and B41 ∆CT expression plasmids were transiently co-transfected with furin plasmids in a 2:1 ratio into 293F cells (Invitrogen), using 293Fectin (Invitrogen), cultured in 293 Freestyle media (Life Technologies) and grown for 72 h at 37 °C. .. Membrane-bound Env trimers were then isolated using PGT151-TEV pull down.

    Article Title: PLAP efficiently generates mature antigenic peptides in vitro but in patterns distinct from ERAP1 1
    Article Snippet: .. Transfection was performed using the 293Fectin transfection reagent according to the freestyle293™ expression system (Invitrogen) instructions. .. Three days post tranfection the cell supernatant was harvested and expression confirmed by L-AMC assay and by western blotting using an anti-5xHis-HRP conjugate antibody (Qiagen).

    Plasmid Preparation:

    Article Title: Epitope determines efficacy of therapeutic anti-Tau antibodies in a functional assay with human Alzheimer Tau
    Article Snippet: .. Preparation of HEK293F cells expressing human P301S Tau HEK293F cells (Thermo Fisher Scientific) were transiently transfected with the pcDNA3.1(+) vector expressing human Tau 2N4R with a P301S mutation, using 293Fectin (Thermo Fisher Scientific) according to the manufacturer’s instructions. .. Cell seeding For western blot analysis, HEK293F cells expressing untagged human 2N4R P301S Tau were plated on 6-well dishes at a density of 1,000,000 cells per well in complete 293 expression medium (Thermo Fisher Scientific) containing 10% fetal bovine serum and 1% Penicillin–Streptomycin.

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  • 91
    Thermo Fisher 293fectin transfection reagent
    293fectin Transfection Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 111 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/293fectin transfection reagent/product/Thermo Fisher
    Average 91 stars, based on 111 article reviews
    Price from $9.99 to $1999.99
    293fectin transfection reagent - by Bioz Stars, 2020-08
    91/100 stars
      Buy from Supplier

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