Journal: Pharmacology Research & Perspectives

Article Title: Lobaric Acid Exhibits Anticancer Potential by Modulating the Wnt/β‐Catenin Signaling Pathway in MCF‐7 Cells

doi: 10.1002/prp2.70142

Figure Lengend Snippet: Effect of LA on Wnt/β‐catenin pathway in MCF‐7 cells. (A) The migration of cells treated with LA into the wound was visualized at 0, 6, 12, and 24 h under an inverted microscope, and the percentage of wound closure was evaluated statistically. (B–I) Expressions of WNT2 , DVL1 , AXIN1 , β‐Catenin , TCF‐4 , CCND1 , c‐MYC , and CDK1 genes analyzed by qPCR in LA‐treated MCF‐7 cells at 48 h. (J–M) Protein expressions of WNT2, GSK3‐ β, and β‐catenin determined by Western blot analysis. The experiment was performed in three biological and technical replicates. * p < 0.05, ** p < 0.01, and *** p < 0.001 in relation to the control. Scale bar, 500 μm.

Article Snippet: Subsequently, membrane incubation was performed overnight at 4°C using P53 antibody (Proteintech, 10442‐1‐AP, 1:1000), BCL2 antibody (Proteintech 12789‐1‐AP 1:500), WNT2 antibody (Proteintech, 66656‐I‐Ig, 1:1000), AXIN antibody (Proteintech, 68093‐1‐Ig, 1:1000), β‐Catenin antibody (Santa Cruz Biotechnology, sc‐7963, 1:1000), GSK3‐ β antibody (Santa Cruz, sc‐377213, 1:1000), and β‐Actin antibody (Santa Cruz Biotechnology, sc‐47778, 1:1000).

Techniques: Migration, Inverted Microscopy, Western Blot, Control

Journal: Parasites & Vectors

Article Title: Excretory/secretory products from Hymenolepis nana adult worms alleviate ulcerative colitis in mice via tuft/IL-13 signaling pathway

doi: 10.1186/s13071-025-06893-x

Figure Lengend Snippet: ESPs promote ISC proliferation in the mouse colon to alleviate UC. A Representative images of IHC for PCNA (brown indicated by red arrowheads in the lower panel, scale bars 100 μm for the upper panel and 50 μm for the lower panel). Representative images of IF for PCNA ( B ) (green) and Lgr5 ( C ) (green) and the nucleus (4′,6-diamidino-2-phenylindole (DAPI), blue) (scale bars 100 μm). Percentages of PCNA-positive area ( D ), the number of PCNA cells ( E ), and ISC ( F ) were semi-quantified using Image J. G The transcription level of Lgr5 was determined using the 2 −ΔΔCt method normalized to GAPDH . H WB results for PCNA and Lgr5 were set as left panel and relative expressions of PCNA and Lgr5 were semi-quantified using Image J as right panel. Data are presented as mean + SD for D – G and the right panel of H . Group size of n = 7 per group. * p < 0.05; ** p < 0.01; *** p < 0.001; ns, not statistically significant

Article Snippet: Primary antibodies for ZO-1 (21773–1-AP), β-tubulin (10094–1-AP), and occludin (66378–1-IG) were offered by Proteintech (Wuhan, China); those for Lgr5 (R380973) were obtained from ZenBio (Chengdu, China); those for IL-13 (A00077-2) were offered by Boster (Wuhan, China); those for GAPDH (AF7021) were purchased from Affinity (Jiangsu, China); those for MUC2 (ab272692), lysozyme (Lyz) (ab108508), MMP7 (ab302893), and Dclk1 (ab31704) were obtained from Abcam (Cambridge, UK); those for Lgr5 (DF2816) were purchased from Affinity (Jiangsu, China); and those for PCNA (PA5-27214, MA5-11358) and fluorescent secondary antibody anti-rabbit 488 (A21206) were offered by Thermo Fisher Scientific (Waltham, MA, USA).

Techniques:

Journal: Parasites & Vectors

Article Title: Excretory/secretory products from Hymenolepis nana adult worms alleviate ulcerative colitis in mice via tuft/IL-13 signaling pathway

doi: 10.1186/s13071-025-06893-x

Figure Lengend Snippet: ESPs promote ISC proliferation in the mouse small intestine to alleviate UC. Representative images of IHC for PCNA ( A ) and Olfm4 ( D ); percentages of PCNA and Olfm4-positive areas were semi-quantified using Image J in A and D (brown indicated by red arrowheads showing PCNA and black arrowheads showing Olfm4 in the lower panels; scale bars 100 μm for the upper panels and 50 μm for the lower panels). Representative images of IF for PCNA ( B ) (green), Olfm4 ( C ) (green), and Lgr5 ( E ) (green) and the nucleus (4′,6-diamidino-2-phenylindole (DAPI), blue) (scale bars 100 μm); percentages of the number of PCNA cells, Olfm4 ISC, and Lgr5 ISC were semi-quantified using Image J in B , C , and E . ( F ) WB result for Lgr5 (left panel) and the relative expression level of Lgr5 (right panel), which was semi-quantified using Image J. ( G ) The transcription level of Lgr5 was determined using the 2 −ΔΔCt method normalized to GAPDH . Data are presented as mean + SD for the right panel of A – F and G . Group size of n = 7 per group. * p < 0.05; ** p < 0.01; *** p < 0.001

Article Snippet: Primary antibodies for ZO-1 (21773–1-AP), β-tubulin (10094–1-AP), and occludin (66378–1-IG) were offered by Proteintech (Wuhan, China); those for Lgr5 (R380973) were obtained from ZenBio (Chengdu, China); those for IL-13 (A00077-2) were offered by Boster (Wuhan, China); those for GAPDH (AF7021) were purchased from Affinity (Jiangsu, China); those for MUC2 (ab272692), lysozyme (Lyz) (ab108508), MMP7 (ab302893), and Dclk1 (ab31704) were obtained from Abcam (Cambridge, UK); those for Lgr5 (DF2816) were purchased from Affinity (Jiangsu, China); and those for PCNA (PA5-27214, MA5-11358) and fluorescent secondary antibody anti-rabbit 488 (A21206) were offered by Thermo Fisher Scientific (Waltham, MA, USA).

Techniques: Expressing

Journal: Parasites & Vectors

Article Title: Excretory/secretory products from Hymenolepis nana adult worms alleviate ulcerative colitis in mice via tuft/IL-13 signaling pathway

doi: 10.1186/s13071-025-06893-x

Figure Lengend Snippet: ESPs enhance ISC proliferation and differentiation to promote intestinal epithelial regeneration and alleviate organoid damage. ( A ) WB results for Lgr5 and PCNA (left panel) and the relative expression levels of Lgr5 and PCNA (right panel) were semi-quantified using Image J. ( B ) WB results for Dclk1, Lyz, and MUC2 (left panel); the relative expression levels of Dclk1, Lyz, and MUC2 were semi-quantified using Image J. The transcription levels of Lgr5 ( C ), Dclk1 ( D ), Lyz1 ( E ), MUC2 ( F ), and IL-13 ( H ) were determined using the 2 −ΔΔCt method normalized to GAPDH . ( G ) WB result for IL-13 (left panel) and the relative expression level of IL-13 was semi-quantified using Image J. Data are presented as mean + SD for the right panels of A , B & G and C – F and H . Group size of n = 3 per group. * p < 0.05; ** p < 0.01; *** p < 0.001

Article Snippet: Primary antibodies for ZO-1 (21773–1-AP), β-tubulin (10094–1-AP), and occludin (66378–1-IG) were offered by Proteintech (Wuhan, China); those for Lgr5 (R380973) were obtained from ZenBio (Chengdu, China); those for IL-13 (A00077-2) were offered by Boster (Wuhan, China); those for GAPDH (AF7021) were purchased from Affinity (Jiangsu, China); those for MUC2 (ab272692), lysozyme (Lyz) (ab108508), MMP7 (ab302893), and Dclk1 (ab31704) were obtained from Abcam (Cambridge, UK); those for Lgr5 (DF2816) were purchased from Affinity (Jiangsu, China); and those for PCNA (PA5-27214, MA5-11358) and fluorescent secondary antibody anti-rabbit 488 (A21206) were offered by Thermo Fisher Scientific (Waltham, MA, USA).

Techniques: Expressing

Journal: Environmental microbiology reports

Article Title: Ecology and Pathogenicity for Honey Bee Brood of Recently Described Paenibacillus melissococcoides and Comparison With Paenibacillus dendritiformis, Paenibacillus thiaminolyticus.

doi: 10.1111/1758-2229.70089

Figure Lengend Snippet: FIGURE 1 | Survival profile of in vitro reared larvae inoculated at the first instar stage with three isolates of Paenibacillus melissococcoides, and one isolate each of Paenibacillus dendritiformis, Paenibacillus thiaminolyticus and Melissococcus plutonius, compared with non-inoculated control larvae. The sample size is indicated in the legend. The asterisks represent significant differences between the groups' survival curves (log-rank tests, p < 0.05, Table S2).

Article Snippet: Three P. melissococcoides isolates, 1.2, 2.1 and 3.2 (Ory et al. 2023), and two closely related Paenibacillus species, P. dendritiformis LMG 21716 (type strain, provided by BCCM LMG) and P. thiaminolyticus DSM 7262 (type strain, provided by DSMZ GmbH) were cultivated on basal medium Petri dishes for four days at 36°C under oxic conditions as recommended in Ory et al. (2023).

Techniques: In Vitro, Control

Journal: Environmental microbiology reports

Article Title: Ecology and Pathogenicity for Honey Bee Brood of Recently Described Paenibacillus melissococcoides and Comparison With Paenibacillus dendritiformis, Paenibacillus thiaminolyticus.

doi: 10.1111/1758-2229.70089

Figure Lengend Snippet: FIGURE 2 | Location of apiaries screened for Paenibacillus melissococcoides in the period 2005–2021 in Switzerland. The number of apiaries an- alysed per year and symptom status is indicated next to the corresponding symbol in the legend: Circles correspond to screened apiaries with EFB- symptomatic colonies, triangles to screened apiaries with asymptomatic colonies, and squares to idiopathic cases. Colours represent the different years. In many occasions, apiaries screened were so close to each other that their symbols overlap completely.

Article Snippet: Three P. melissococcoides isolates, 1.2, 2.1 and 3.2 (Ory et al. 2023), and two closely related Paenibacillus species, P. dendritiformis LMG 21716 (type strain, provided by BCCM LMG) and P. thiaminolyticus DSM 7262 (type strain, provided by DSMZ GmbH) were cultivated on basal medium Petri dishes for four days at 36°C under oxic conditions as recommended in Ory et al. (2023).

Techniques:

Journal: Environmental microbiology reports

Article Title: Ecology and Pathogenicity for Honey Bee Brood of Recently Described Paenibacillus melissococcoides and Comparison With Paenibacillus dendritiformis, Paenibacillus thiaminolyticus.

doi: 10.1111/1758-2229.70089

Figure Lengend Snippet: FIGURE 3 | Paenibacillus melissococcoides pathogenicity to honey bee brood. (A) Assay design and corresponding steps of the Koch's postulates (in bold) testing the pathogenicity of P. melissococcoides at the individual level. Also shown are the results of MALDI-TOF mass-spectrometry identi- fication (column ‘organism’) of P. melissococcoides after sequential isolation and re-isolation from larvae that died following inoculation with isolate 1.2 (column ‘strain’). Score value interpretation: 3.000–2.300: Highly probable species identification; 2.299–2.000: Secure genus identification, prob- able species identification; 1.999–1.700: Probable genus identification; 1.699–0.000: Unreliable identification. (B) Survival profile of in vitro reared larvae inoculated at first instar stage with P. melissococcoides 1.2, which was reisolated from a previously inoculated dead fifth instar larva. Survival was compared with non-inoculated control larvae. The sample size is indicated in the legend. The asterisk represents a significant difference between the groups' survival curves (log-rank test).

Article Snippet: Three P. melissococcoides isolates, 1.2, 2.1 and 3.2 (Ory et al. 2023), and two closely related Paenibacillus species, P. dendritiformis LMG 21716 (type strain, provided by BCCM LMG) and P. thiaminolyticus DSM 7262 (type strain, provided by DSMZ GmbH) were cultivated on basal medium Petri dishes for four days at 36°C under oxic conditions as recommended in Ory et al. (2023).

Techniques: Mass Spectrometry, Isolation, In Vitro, Control

Journal: Environmental microbiology reports

Article Title: Ecology and Pathogenicity for Honey Bee Brood of Recently Described Paenibacillus melissococcoides and Comparison With Paenibacillus dendritiformis, Paenibacillus thiaminolyticus.

doi: 10.1111/1758-2229.70089

Figure Lengend Snippet: FIGURE 4 | Survival profiles of in vitro reared larvae inoculated with Paenibacillus melissococcoides 3.2 and of non-inoculated control larvae. (A) Dose dependence: One-day-old larvae inoculated on the first day of rearing with different doses of P. melissococcoides (in CFUs) and controls. (B) Age dependence: One-, three- and five-day-old larvae inoculated with 2 × 105 P. melissococcoides bacteria (arrows), respectively, and controls. The sample size is indicated in the legend. Different letters to the right of the curves indicate significant differences in the survival of the tested dose and age groups (pairwise log-rank tests, Bonferroni-Holm corrected, p < 0.05).

Article Snippet: Three P. melissococcoides isolates, 1.2, 2.1 and 3.2 (Ory et al. 2023), and two closely related Paenibacillus species, P. dendritiformis LMG 21716 (type strain, provided by BCCM LMG) and P. thiaminolyticus DSM 7262 (type strain, provided by DSMZ GmbH) were cultivated on basal medium Petri dishes for four days at 36°C under oxic conditions as recommended in Ory et al. (2023).

Techniques: In Vitro, Control, Bacteria