kit no 27104  (Qiagen)


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    Structured Review

    Qiagen kit no 27104
    Kit No 27104, supplied by Qiagen, used in various techniques. Bioz Stars score: 81/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/kit no 27104/product/Qiagen
    Average 81 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    kit no 27104 - by Bioz Stars, 2020-01
    81/100 stars

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    Clone Assay:

    Article Title: Ubiquitously expressed transcript is a novel interacting protein of protein inhibitor of activated signal transducer and activator of transcription 2
    Article Snippet: .. Plasmid DNA of prey clones was isolated using QIAprep Spin Miniprep kit according to the manufacturer’s instructions (cat no. 27104; Qiagen, Hilden, Germany) and transformed into E. coli DH5α (cat no. D9057; Takara Bio Inc. Otsu, Japan). .. Subsequently, prey clones were recovered in the lysogeny broth medium (Thermo Fisher Scientific, Inc., Waltham, MA, USA) containing ampicillin. and cDNA inserts were amplified by PCR using the Advantage 2 PCR kit (cat no. 639207; Clontech Laboratories, Inc.).

    Amplification:

    Article Title: Ubiquitously expressed transcript is a novel interacting protein of protein inhibitor of activated signal transducer and activator of transcription 2
    Article Snippet: Plasmid DNA of prey clones was isolated using QIAprep Spin Miniprep kit according to the manufacturer’s instructions (cat no. 27104; Qiagen, Hilden, Germany) and transformed into E. coli DH5α (cat no. D9057; Takara Bio Inc. Otsu, Japan). .. Subsequently, prey clones were recovered in the lysogeny broth medium (Thermo Fisher Scientific, Inc., Waltham, MA, USA) containing ampicillin. and cDNA inserts were amplified by PCR using the Advantage 2 PCR kit (cat no. 639207; Clontech Laboratories, Inc.).

    Isolation:

    Article Title: Ubiquitously expressed transcript is a novel interacting protein of protein inhibitor of activated signal transducer and activator of transcription 2
    Article Snippet: .. Plasmid DNA of prey clones was isolated using QIAprep Spin Miniprep kit according to the manufacturer’s instructions (cat no. 27104; Qiagen, Hilden, Germany) and transformed into E. coli DH5α (cat no. D9057; Takara Bio Inc. Otsu, Japan). .. Subsequently, prey clones were recovered in the lysogeny broth medium (Thermo Fisher Scientific, Inc., Waltham, MA, USA) containing ampicillin. and cDNA inserts were amplified by PCR using the Advantage 2 PCR kit (cat no. 639207; Clontech Laboratories, Inc.).

    Activation Assay:

    Article Title: Ubiquitously expressed transcript is a novel interacting protein of protein inhibitor of activated signal transducer and activator of transcription 2
    Article Snippet: Secretion of this enzyme in response to GAL4 activation leads to hydrolysis of X-α-Gal in the medium, resulting in yeast colonies developing a blue color. .. Plasmid DNA of prey clones was isolated using QIAprep Spin Miniprep kit according to the manufacturer’s instructions (cat no. 27104; Qiagen, Hilden, Germany) and transformed into E. coli DH5α (cat no. D9057; Takara Bio Inc. Otsu, Japan).

    Expressing:

    Article Title: Ubiquitously expressed transcript is a novel interacting protein of protein inhibitor of activated signal transducer and activator of transcription 2
    Article Snippet: When cotransformed pGBKT7-p53 and pGADT7-SV40 into AH109, they can interact and activate transcription of and thus induce expression of the reporter genes (ADE2, HIS3, lacZ, and MEL1). .. Plasmid DNA of prey clones was isolated using QIAprep Spin Miniprep kit according to the manufacturer’s instructions (cat no. 27104; Qiagen, Hilden, Germany) and transformed into E. coli DH5α (cat no. D9057; Takara Bio Inc. Otsu, Japan).

    Polymerase Chain Reaction:

    Article Title: Ubiquitously expressed transcript is a novel interacting protein of protein inhibitor of activated signal transducer and activator of transcription 2
    Article Snippet: Plasmid DNA of prey clones was isolated using QIAprep Spin Miniprep kit according to the manufacturer’s instructions (cat no. 27104; Qiagen, Hilden, Germany) and transformed into E. coli DH5α (cat no. D9057; Takara Bio Inc. Otsu, Japan). .. Subsequently, prey clones were recovered in the lysogeny broth medium (Thermo Fisher Scientific, Inc., Waltham, MA, USA) containing ampicillin. and cDNA inserts were amplified by PCR using the Advantage 2 PCR kit (cat no. 639207; Clontech Laboratories, Inc.).

    Transformation Assay:

    Article Title: Ubiquitously expressed transcript is a novel interacting protein of protein inhibitor of activated signal transducer and activator of transcription 2
    Article Snippet: .. Plasmid DNA of prey clones was isolated using QIAprep Spin Miniprep kit according to the manufacturer’s instructions (cat no. 27104; Qiagen, Hilden, Germany) and transformed into E. coli DH5α (cat no. D9057; Takara Bio Inc. Otsu, Japan). .. Subsequently, prey clones were recovered in the lysogeny broth medium (Thermo Fisher Scientific, Inc., Waltham, MA, USA) containing ampicillin. and cDNA inserts were amplified by PCR using the Advantage 2 PCR kit (cat no. 639207; Clontech Laboratories, Inc.).

    Plasmid Preparation:

    Article Title: Ubiquitously expressed transcript is a novel interacting protein of protein inhibitor of activated signal transducer and activator of transcription 2
    Article Snippet: .. Plasmid DNA of prey clones was isolated using QIAprep Spin Miniprep kit according to the manufacturer’s instructions (cat no. 27104; Qiagen, Hilden, Germany) and transformed into E. coli DH5α (cat no. D9057; Takara Bio Inc. Otsu, Japan). .. Subsequently, prey clones were recovered in the lysogeny broth medium (Thermo Fisher Scientific, Inc., Waltham, MA, USA) containing ampicillin. and cDNA inserts were amplified by PCR using the Advantage 2 PCR kit (cat no. 639207; Clontech Laboratories, Inc.).

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  • 90
    Qiagen qiaprep spin miniprep kit
    Qiaprep Spin Miniprep Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 3423 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qiaprep spin miniprep kit/product/Qiagen
    Average 90 stars, based on 3423 article reviews
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