2611l phusion hot start flex dna polymerase new england biolabs cat  (New England Biolabs)


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  • 99
    Name:
    Gibson Assembly Master Mix
    Description:
    Gibson Assembly Master Mix 50 rxns
    Catalog Number:
    e2611l
    Price:
    649
    Size:
    50 rxns
    Category:
    Cloning and Expression Systems
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    Structured Review

    New England Biolabs 2611l phusion hot start flex dna polymerase new england biolabs cat
    Gibson Assembly Master Mix
    Gibson Assembly Master Mix 50 rxns
    https://www.bioz.com/result/2611l phusion hot start flex dna polymerase new england biolabs cat/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    2611l phusion hot start flex dna polymerase new england biolabs cat - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: CRISPR-based gene replacement reveals evolutionarily conserved axon guidance functions of Drosophila Robo3 and Tribolium Robo2/3
    Article Snippet: .. Construction of robo3TcRobo2/3 donor plasmid The initial robo3 donor construct was assembled from four PCR fragments via Gibson assembly (New England Biolabs E2611). .. The four fragments were derived from pBluescript (plasmid backbone; primer pair 417–418), the wild-type robo3 genomic locus (5′ and 3′ homology regions; primer pairs 501–502 and 505–506), and the robo3 cDNA (robo3 coding region; primer pair 503–504).

    Clone Assay:

    Article Title: TPXL-1 activates Aurora A to clear contractile ring components from the polar cortex during cytokinesis
    Article Snippet: .. Transgene construction Gibson cloning (E2611; NEB) was used to construct transgenes encoding WT and mNeonGreen tagged TPXL-1 (isoform A) in pCFJ350. ..

    Article Title: Discrete and Structurally Unique Proteins (Tāpirins) Mediate Attachment of Extremely Thermophilic Caldicellulosiruptor Species to Cellulose *
    Article Snippet: .. Tāpirins (Calkro_0844, Calkro_0845) were cloned into the vector pCTCON , using conventional ligation (Calkro_0845) or Gibson Assembly® master mix (Calkro_0844) (New England Biolabs), according to the manufacturer's directions. .. The cloning strategy excluded signal peptides and/or transmembrane domains; oligonucleotide primers used for cloning are listed in .

    Article Title: Temperature-dependent regulation of upstream open reading frame translation in S. cerevisiae
    Article Snippet: .. Generation of C-terminally HA-tagged clones of AGA1 gene Gibson assembly master mix (NEB# E2611) was used to generate C-terminally HA-tagged AGA1 clones [AGA 1-HA (WT), see plasmid #12, Additional file : Table S2]. ..

    Construct:

    Article Title: TPXL-1 activates Aurora A to clear contractile ring components from the polar cortex during cytokinesis
    Article Snippet: .. Transgene construction Gibson cloning (E2611; NEB) was used to construct transgenes encoding WT and mNeonGreen tagged TPXL-1 (isoform A) in pCFJ350. ..

    Article Title: CRISPR-based gene replacement reveals evolutionarily conserved axon guidance functions of Drosophila Robo3 and Tribolium Robo2/3
    Article Snippet: .. Construction of robo3TcRobo2/3 donor plasmid The initial robo3 donor construct was assembled from four PCR fragments via Gibson assembly (New England Biolabs E2611). .. The four fragments were derived from pBluescript (plasmid backbone; primer pair 417–418), the wild-type robo3 genomic locus (5′ and 3′ homology regions; primer pairs 501–502 and 505–506), and the robo3 cDNA (robo3 coding region; primer pair 503–504).

    Plasmid Preparation:

    Article Title: Discrete and Structurally Unique Proteins (Tāpirins) Mediate Attachment of Extremely Thermophilic Caldicellulosiruptor Species to Cellulose *
    Article Snippet: .. Tāpirins (Calkro_0844, Calkro_0845) were cloned into the vector pCTCON , using conventional ligation (Calkro_0845) or Gibson Assembly® master mix (Calkro_0844) (New England Biolabs), according to the manufacturer's directions. .. The cloning strategy excluded signal peptides and/or transmembrane domains; oligonucleotide primers used for cloning are listed in .

    Article Title: Temperature-dependent regulation of upstream open reading frame translation in S. cerevisiae
    Article Snippet: .. Generation of C-terminally HA-tagged clones of AGA1 gene Gibson assembly master mix (NEB# E2611) was used to generate C-terminally HA-tagged AGA1 clones [AGA 1-HA (WT), see plasmid #12, Additional file : Table S2]. ..

    Article Title: CRISPR-based gene replacement reveals evolutionarily conserved axon guidance functions of Drosophila Robo3 and Tribolium Robo2/3
    Article Snippet: .. Construction of robo3TcRobo2/3 donor plasmid The initial robo3 donor construct was assembled from four PCR fragments via Gibson assembly (New England Biolabs E2611). .. The four fragments were derived from pBluescript (plasmid backbone; primer pair 417–418), the wild-type robo3 genomic locus (5′ and 3′ homology regions; primer pairs 501–502 and 505–506), and the robo3 cDNA (robo3 coding region; primer pair 503–504).

    Ligation:

    Article Title: Discrete and Structurally Unique Proteins (Tāpirins) Mediate Attachment of Extremely Thermophilic Caldicellulosiruptor Species to Cellulose *
    Article Snippet: .. Tāpirins (Calkro_0844, Calkro_0845) were cloned into the vector pCTCON , using conventional ligation (Calkro_0845) or Gibson Assembly® master mix (Calkro_0844) (New England Biolabs), according to the manufacturer's directions. .. The cloning strategy excluded signal peptides and/or transmembrane domains; oligonucleotide primers used for cloning are listed in .

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  • 99
    New England Biolabs 2611l phusion hot start flex dna polymerase new england biolabs cat
    2611l Phusion Hot Start Flex Dna Polymerase New England Biolabs Cat, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/2611l phusion hot start flex dna polymerase new england biolabs cat/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    2611l phusion hot start flex dna polymerase new england biolabs cat - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

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