mirneasy mini kit  (Qiagen)


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    Name:
    miRNeasy Mini Kit
    Description:
    For purification of microRNA and total RNA from tissues and cells Kit contents Qiagen miRNeasy Mini Kit 50 preps 50 to 100mg Sample 30L Elution Volume Tissue Cells Sample miRNA Total RNA Purification Spin Column Format Silica Technology Ideal for PCR qPCR Real time RT PCR Microarray For Purification of microRNA and Total RNA from Tissues and Cells Includes 50 RNeasy Mini Spin Columns Collection Tubes 1 5 and 2mL QIAzol Lysis Reagent RNase free Reagents and Buffers Benefits Effective purification of miRNA and total RNA Efficient enrichment of miRNA and RNAs 200 nucleotides High purity RNA suitable for all downstream applications Protocols for copurification or isolation of separate fractio
    Catalog Number:
    217004
    Price:
    368
    Category:
    miRNeasy Mini Kit
    Buy from Supplier


    Structured Review

    Qiagen mirneasy mini kit
    miRNeasy Mini Kit
    For purification of microRNA and total RNA from tissues and cells Kit contents Qiagen miRNeasy Mini Kit 50 preps 50 to 100mg Sample 30L Elution Volume Tissue Cells Sample miRNA Total RNA Purification Spin Column Format Silica Technology Ideal for PCR qPCR Real time RT PCR Microarray For Purification of microRNA and Total RNA from Tissues and Cells Includes 50 RNeasy Mini Spin Columns Collection Tubes 1 5 and 2mL QIAzol Lysis Reagent RNase free Reagents and Buffers Benefits Effective purification of miRNA and total RNA Efficient enrichment of miRNA and RNAs 200 nucleotides High purity RNA suitable for all downstream applications Protocols for copurification or isolation of separate fractio
    https://www.bioz.com/result/mirneasy mini kit/product/Qiagen
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    mirneasy mini kit - by Bioz Stars, 2020-01
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    Related Articles

    Clone Assay:

    Article Title: miR-3656 expression enhances the chemosensitivity of pancreatic cancer to gemcitabine through modulation of the RHOF/EMT axis
    Article Snippet: .. miRNA expression profiling Total miRNAs were isolated from the parental PANC-1 clone and three independent GR PANC-1 clones using miRNeasy Mini Kit according to the instructions of the manufacturer (Qiagen, Valencia, CA, USA). .. The miRNA expression profiling was performed by using the miRCURY LNA expression array (Exiqon, Vedbaek, Denmark).

    Amplification:

    Article Title: Whole Genome Expression Analyses of miRNAs and mRNAs Suggest the Involvement of miR-320a and miR-155-3p and their Targeted Genes in Lithium Response in Bipolar Disorder
    Article Snippet: Genome-Wide NGS Analysis of miRNAs Total RNA was extracted using the miRNeasy Mini Kit (QIAGEN GmbH-Hilden, Hilden, Germany). .. During library preparation, small RNAs having the 3′ hydroxyl end and the 5′-phosphate group produced by Dicer were selectively ligated to adapters, retrotranscribed, amplified and indexed, and finally purified to produce pure miRNA libraries.

    Synthesized:

    Article Title: mTOR and autophagy pathways are dysregulated in murine and human models of Schaaf-Yang syndrome
    Article Snippet: .. RNA isolation and qRT-PCR RNA isolation was performed using the miRNeasy mini kit (QIAGEN) according to manufacturer’s instructions. cDNA was synthesized using M-MLV Reverse Transcriptase (Invitrogen) according to the manufacturer’s directions. qRT-PCR reactions were run in triplicate using SYBER Green master mix (BioRad) according to manufacturer’s protocol. qRT-PCR was performed using the CFX96 Real Time System (Bio-Rad). qRT-PCR primer sequences are provided in Supplemental Table . .. Immunoblotting Human fibroblasts were rinsed once with cold PBS and harvested with NP-40 lysis buffer, supplemented with 1X protease and phosphatase inhibitors (Thermo Fisher Scientific).

    Quantitative RT-PCR:

    Article Title: High-glucose-induced miR-214-3p inhibits BMSCs osteogenic differentiation in type 1 diabetes mellitus
    Article Snippet: SYBR Premix Ex Taq kit (Takara Biological Incorporated Company, Kyoto, Japan) was used for qRT-PCR analysis using the Bio-Rad iQ5 real-time PCR system (Bio-Rad, Hercules, CA, USA). .. The miRNeasy Mini Kit (QIAGEN, USA) was used to extract miRNAs from bone specimens or BMSCs.

    Article Title: MicroRNA-448 modulates the progression of neuropathic pain by targeting sirtuin 1
    Article Snippet: .. RNA extraction and reverse-transcription quantitative polymerase chain reaction (RT-qPCR) Total RNA was isolated from tissues or cells using a miRNeasy mini kit (Qiagen) according to the manufacturer's protocol. .. RT was performed using TaqMan miRNA reverse-transcription kit (Applied Biosystems; Thermo Fisher Scientific, Inc.) according to the manufacturer's protocol. qPCR was conducted to detect the expression levels of miR-448 and SIRT1 using a SYBR Premix Ex Taq™ II commercial kit (Takara Biotechnology Co. Ltd.) and the Applied Biosystems 7900 Real-Time PCR System (Applied Biosystems; Thermo Fisher Scientific, Inc.) according to the manufacturer's protocol.

    Article Title: mTOR and autophagy pathways are dysregulated in murine and human models of Schaaf-Yang syndrome
    Article Snippet: .. RNA isolation and qRT-PCR RNA isolation was performed using the miRNeasy mini kit (QIAGEN) according to manufacturer’s instructions. cDNA was synthesized using M-MLV Reverse Transcriptase (Invitrogen) according to the manufacturer’s directions. qRT-PCR reactions were run in triplicate using SYBER Green master mix (BioRad) according to manufacturer’s protocol. qRT-PCR was performed using the CFX96 Real Time System (Bio-Rad). qRT-PCR primer sequences are provided in Supplemental Table . .. Immunoblotting Human fibroblasts were rinsed once with cold PBS and harvested with NP-40 lysis buffer, supplemented with 1X protease and phosphatase inhibitors (Thermo Fisher Scientific).

    Article Title: Expression and significance of miR - 20b in retinal photoreceptor cells exposed to PCB1254
    Article Snippet: Paragraph title: Real-time quantitative polymerase chain reaction (RT-qPCR) assay ... For miR-20b, miRNeasy Mini Kit (Qiagen Inc., Valencia, USA) was used for RNA reverse transcription.

    SYBR Green Assay:

    Article Title: Ube3a-ATS is an atypical RNA polymerase II transcript that represses the paternal expression of Ube3a
    Article Snippet: Total RNA was prepared with miRNeasy Mini Kit (Qiagen, Valencia, CA, USA). .. The cDNA was then generated using 0.2–1 μg of total RNA with SuperScript III First-Strand Synthesis System (Invitrogen), and q-PCR was performed using Applied Biosystems StepOnePlus Real-Time PCR System and SYBR Green Master Mix (Applied Biosystems, Carlsbad, CA, USA).

    Article Title: p53 Represses the Oncogenic Sno-MiR-28 Derived from a SnoRNA
    Article Snippet: Real-time PCR reaction was performed on a CFX Connect Real-Time PCR Detection System (Bio-Rad) using iTaq Universal SYBR Green Supermix (Bio-Rad). .. Small RNAs (including miRNAs and sno-miRNAs) were extracted using the miRNeasy mini kit (Qiagen) following the manufacturer’s instructions.

    Article Title: Expression and significance of miR - 20b in retinal photoreceptor cells exposed to PCB1254
    Article Snippet: For miR-20b, miRNeasy Mini Kit (Qiagen Inc., Valencia, USA) was used for RNA reverse transcription. .. Quantitative real-time RT-qPCR was performed with PowerUp SYBR Green Master Mix (Thermo Fisher Scientific, USA).

    Microarray:

    Article Title: MicroRNA-132-3p suppresses type I IFN response through targeting IRF1 to facilitate H1N1 influenza A virus infection
    Article Snippet: .. MicroRNA microarray analysis RNA was extracted from peripheral blood samples from three influenza patients and three healthy controls using an miRNeasy Mini Kit (QIAGEN GmbH, Hilden, Germany) and then the RNA concentration were analyzed with an NanoDrop ND-2000 spectrophotometry (Thermo Fisher Scientific, Inc., Waltham, MA, U.S.A.). .. About 1 μg of total RNA was used as the input for the labeling reaction and hybridized using the miRCURY Hy3/Hy5 Power Labeling kit and miRCURY™ LNA array (v.16.0; Exiqon A/S, Copenhagen, Denmark) according to the manufacturer’s protocol.

    Article Title: Dietary delphinidin inhibits human colorectal cancer metastasis associating with upregulation of miR-204-3p and suppression of the integrin/FAK axis
    Article Snippet: Paragraph title: Microarray analysis for miR profiling ... In brief, total RNA from DLD-1 cells was extracted using the miRNeasy mini kit (QIAGEN, Mainz, Germany) and labeled with Cy3 during reverse transcription to cDNA using an Agilent miRNA Labeling Kit (Agilent, UK) and Spike Kit (Agilent, UK).

    TLDA Assay:

    Article Title: Comparison of different extraction techniques to profile microRNAs from human sera and peripheral blood mononuclear cells
    Article Snippet: .. Total RNAs were extracted from 1×106 or 3×106 PBMCs using the Qiagen miRNeasy mini kit and further profiled with TLDA cards. ..

    Expressing:

    Article Title: miR-3656 expression enhances the chemosensitivity of pancreatic cancer to gemcitabine through modulation of the RHOF/EMT axis
    Article Snippet: .. miRNA expression profiling Total miRNAs were isolated from the parental PANC-1 clone and three independent GR PANC-1 clones using miRNeasy Mini Kit according to the instructions of the manufacturer (Qiagen, Valencia, CA, USA). .. The miRNA expression profiling was performed by using the miRCURY LNA expression array (Exiqon, Vedbaek, Denmark).

    Article Title: High-glucose-induced miR-214-3p inhibits BMSCs osteogenic differentiation in type 1 diabetes mellitus
    Article Snippet: Fold changes in expression were calculated by the 2−ΔΔCt method. .. The miRNeasy Mini Kit (QIAGEN, USA) was used to extract miRNAs from bone specimens or BMSCs.

    Article Title: MicroRNA-448 modulates the progression of neuropathic pain by targeting sirtuin 1
    Article Snippet: RNA extraction and reverse-transcription quantitative polymerase chain reaction (RT-qPCR) Total RNA was isolated from tissues or cells using a miRNeasy mini kit (Qiagen) according to the manufacturer's protocol. .. RT was performed using TaqMan miRNA reverse-transcription kit (Applied Biosystems; Thermo Fisher Scientific, Inc.) according to the manufacturer's protocol. qPCR was conducted to detect the expression levels of miR-448 and SIRT1 using a SYBR Premix Ex Taq™ II commercial kit (Takara Biotechnology Co. Ltd.) and the Applied Biosystems 7900 Real-Time PCR System (Applied Biosystems; Thermo Fisher Scientific, Inc.) according to the manufacturer's protocol.

    Article Title: p53 Represses the Oncogenic Sno-MiR-28 Derived from a SnoRNA
    Article Snippet: In this study, RNA ( > 250bp) expression was normalized to PSMB4 ( ). .. Small RNAs (including miRNAs and sno-miRNAs) were extracted using the miRNeasy mini kit (Qiagen) following the manufacturer’s instructions.

    Genome Wide:

    Article Title: Whole Genome Expression Analyses of miRNAs and mRNAs Suggest the Involvement of miR-320a and miR-155-3p and their Targeted Genes in Lithium Response in Bipolar Disorder
    Article Snippet: .. Genome-Wide NGS Analysis of miRNAs Total RNA was extracted using the miRNeasy Mini Kit (QIAGEN GmbH-Hilden, Hilden, Germany). .. Quantification and quality evaluation of miRNAs was performed using the Small RNA Analysis Kit (DNF-470) adjusted for the Fragment Analyzer™ Automated CE System (Advanced Analytical Technologies, Ankeny, IA, USA) and the Qubit RNA BR (Broad-Range) Assay Kit for Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA).

    Transformation Assay:

    Article Title: Dietary delphinidin inhibits human colorectal cancer metastasis associating with upregulation of miR-204-3p and suppression of the integrin/FAK axis
    Article Snippet: In brief, total RNA from DLD-1 cells was extracted using the miRNeasy mini kit (QIAGEN, Mainz, Germany) and labeled with Cy3 during reverse transcription to cDNA using an Agilent miRNA Labeling Kit (Agilent, UK) and Spike Kit (Agilent, UK). .. Data were normalized using the Plier algorithm and were log transformed and analyzed using GeneSpring GX 13.0 (Agilent, UK). miRs with P values less than 0.05 and fold changes > 2 or < −2 between control (DMSO) and delphinidin-treated cells were considered significantly changed.

    Hybridization:

    Article Title: Dietary delphinidin inhibits human colorectal cancer metastasis associating with upregulation of miR-204-3p and suppression of the integrin/FAK axis
    Article Snippet: In brief, total RNA from DLD-1 cells was extracted using the miRNeasy mini kit (QIAGEN, Mainz, Germany) and labeled with Cy3 during reverse transcription to cDNA using an Agilent miRNA Labeling Kit (Agilent, UK) and Spike Kit (Agilent, UK). .. The labeled cDNAs were hybridized (in duplicate) to Human miRNA Microarray, Release 19.0, 8 × 60 K (v19) microarray slides (Agilent, UK) according to the microRNA Hybridization Kit protocol (Agilent, UK) and scanned using a Nimblegen MS200 array scanner.

    Concentration Assay:

    Article Title: MicroRNA-132-3p suppresses type I IFN response through targeting IRF1 to facilitate H1N1 influenza A virus infection
    Article Snippet: .. MicroRNA microarray analysis RNA was extracted from peripheral blood samples from three influenza patients and three healthy controls using an miRNeasy Mini Kit (QIAGEN GmbH, Hilden, Germany) and then the RNA concentration were analyzed with an NanoDrop ND-2000 spectrophotometry (Thermo Fisher Scientific, Inc., Waltham, MA, U.S.A.). .. About 1 μg of total RNA was used as the input for the labeling reaction and hybridized using the miRCURY Hy3/Hy5 Power Labeling kit and miRCURY™ LNA array (v.16.0; Exiqon A/S, Copenhagen, Denmark) according to the manufacturer’s protocol.

    Article Title: Circular RNAs in Clear Cell Renal Cell Carcinoma: Their Microarray-Based Identification, Analytical Validation, and Potential Use in a Clinico-Genomic Model to Improve Prognostic Accuracy
    Article Snippet: The isolation procedure from preserved tissue specimens using the miRNeasy Mini Kit (Qiagen) including an on-column DNA digestion step according to the producer's instructions, the spectrophotometric quantification (NanoDrop 1000 Spectrophotometer; NanoDrop Technologies, Wilmington, DE, USA), and the quality assessment of the total RNA samples (ratio of the absorbance at 260 nm to that at 280 nm and RNA integrity number on a Bioanalyzer 21000 (Agilent Technologies, Santa Clara, CA, USA) were detailed described in our previous publications [ , , ]. .. The median RNA concentration in the isolates of 30 µL nuclease-free water was 1118 (95% CI, 1029 to 1209) ng/µL).

    Article Title: p53 Represses the Oncogenic Sno-MiR-28 Derived from a SnoRNA
    Article Snippet: Small RNAs (including miRNAs and sno-miRNAs) were extracted using the miRNeasy mini kit (Qiagen) following the manufacturer’s instructions. .. After extraction, total RNA concentration was determined using a ND-1000 NanoDrop spectrometer (Thermo Scientific).

    Article Title: Selective Histone Deacetylase 6 Inhibition Normalizes B Cell Activation and Germinal Center Formation in a Model of Systemic Lupus Erythematosus
    Article Snippet: mRNA-Isolation and Sequencing Total RNA was isolated from whole splenocytes using the miRNeasy Mini Kit (Qiagen, Germantown, MD, USA) per manufacturer's instructions. .. The RNA concentration was quantified using a NanoDrop 2000.

    Introduce:

    Article Title: Comparison of different extraction techniques to profile microRNAs from human sera and peripheral blood mononuclear cells
    Article Snippet: To investigate whether these observations were limited to the MN kit or whether RNA extraction with the Qiagen kit could introduce similar bias. .. Total RNAs were extracted from 1×106 or 3×106 PBMCs using the Qiagen miRNeasy mini kit and further profiled with TLDA cards.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: p53 Represses the Oncogenic Sno-MiR-28 Derived from a SnoRNA
    Article Snippet: The RT-PCR program was: 95°C for 3min, then start cycles consisting 95°C for 10s and 57/60/61°C for 60s (depending on the primers used) for 40 cycles. .. Small RNAs (including miRNAs and sno-miRNAs) were extracted using the miRNeasy mini kit (Qiagen) following the manufacturer’s instructions.

    Generated:

    Article Title: Ube3a-ATS is an atypical RNA polymerase II transcript that represses the paternal expression of Ube3a
    Article Snippet: Total RNA was prepared with miRNeasy Mini Kit (Qiagen, Valencia, CA, USA). .. The cDNA was then generated using 0.2–1 μg of total RNA with SuperScript III First-Strand Synthesis System (Invitrogen), and q-PCR was performed using Applied Biosystems StepOnePlus Real-Time PCR System and SYBR Green Master Mix (Applied Biosystems, Carlsbad, CA, USA).

    Sequencing:

    Article Title: LATS1 but not LATS2 represses autophagy by a kinase-independent scaffold function
    Article Snippet: .. RNA extraction and sequencing analysis RNA from siRNA-transfected and Srf-treated cells was extracted in biological triplicates using miRNeasy Mini kit (Qiagen) according to the manufacturer’s instructions. ..

    Article Title: Selective Histone Deacetylase 6 Inhibition Normalizes B Cell Activation and Germinal Center Formation in a Model of Systemic Lupus Erythematosus
    Article Snippet: .. mRNA-Isolation and Sequencing Total RNA was isolated from whole splenocytes using the miRNeasy Mini Kit (Qiagen, Germantown, MD, USA) per manufacturer's instructions. .. To remove residual amounts of DNA contamination in isolated RNA, on-column DNase digestion with RNase-Free DNase was performed.

    RNA Sequencing Assay:

    Article Title: Selective Histone Deacetylase 6 Inhibition Normalizes B Cell Activation and Germinal Center Formation in a Model of Systemic Lupus Erythematosus
    Article Snippet: mRNA-Isolation and Sequencing Total RNA was isolated from whole splenocytes using the miRNeasy Mini Kit (Qiagen, Germantown, MD, USA) per manufacturer's instructions. .. Total RNA was sent to Beckman Coulter (Danvers, MA, USA) for 2 × 100 bp paired-end Illumina RNA sequencing with an average of 40 million reads per sample.

    Isolation:

    Article Title: miR-3656 expression enhances the chemosensitivity of pancreatic cancer to gemcitabine through modulation of the RHOF/EMT axis
    Article Snippet: .. miRNA expression profiling Total miRNAs were isolated from the parental PANC-1 clone and three independent GR PANC-1 clones using miRNeasy Mini Kit according to the instructions of the manufacturer (Qiagen, Valencia, CA, USA). .. The miRNA expression profiling was performed by using the miRCURY LNA expression array (Exiqon, Vedbaek, Denmark).

    Article Title: Ube3a-ATS is an atypical RNA polymerase II transcript that represses the paternal expression of Ube3a
    Article Snippet: Paragraph title: RNA isolation and q-PCR ... Total RNA was prepared with miRNeasy Mini Kit (Qiagen, Valencia, CA, USA).

    Article Title: Circular RNAs in Clear Cell Renal Cell Carcinoma: Their Microarray-Based Identification, Analytical Validation, and Potential Use in a Clinico-Genomic Model to Improve Prognostic Accuracy
    Article Snippet: .. The isolation procedure from preserved tissue specimens using the miRNeasy Mini Kit (Qiagen) including an on-column DNA digestion step according to the producer's instructions, the spectrophotometric quantification (NanoDrop 1000 Spectrophotometer; NanoDrop Technologies, Wilmington, DE, USA), and the quality assessment of the total RNA samples (ratio of the absorbance at 260 nm to that at 280 nm and RNA integrity number on a Bioanalyzer 21000 (Agilent Technologies, Santa Clara, CA, USA) were detailed described in our previous publications [ , , ]. .. The median ratio of 260 nm to 280 nm of the isolated RNA samples was 2.04 (95% CI, 2.03 to 2.04) and the median RNA integrity number was 7.70 (95% CI, 7.52 to 7.88).

    Article Title: Comparison of different extraction techniques to profile microRNAs from human sera and peripheral blood mononuclear cells
    Article Snippet: A Bland-Altman plot of their Ct values showed that the miRNAs detected using RNA isolated from 3×106 cells had lower Ct values, with a mean difference in Ct of −1.46 compared to 1×106 cells extraction (Figure A), reminiscent of the results obtained with sera. .. Total RNAs were extracted from 1×106 or 3×106 PBMCs using the Qiagen miRNeasy mini kit and further profiled with TLDA cards.

    Article Title: MicroRNA-448 modulates the progression of neuropathic pain by targeting sirtuin 1
    Article Snippet: .. RNA extraction and reverse-transcription quantitative polymerase chain reaction (RT-qPCR) Total RNA was isolated from tissues or cells using a miRNeasy mini kit (Qiagen) according to the manufacturer's protocol. .. RT was performed using TaqMan miRNA reverse-transcription kit (Applied Biosystems; Thermo Fisher Scientific, Inc.) according to the manufacturer's protocol. qPCR was conducted to detect the expression levels of miR-448 and SIRT1 using a SYBR Premix Ex Taq™ II commercial kit (Takara Biotechnology Co. Ltd.) and the Applied Biosystems 7900 Real-Time PCR System (Applied Biosystems; Thermo Fisher Scientific, Inc.) according to the manufacturer's protocol.

    Article Title: mTOR and autophagy pathways are dysregulated in murine and human models of Schaaf-Yang syndrome
    Article Snippet: .. RNA isolation and qRT-PCR RNA isolation was performed using the miRNeasy mini kit (QIAGEN) according to manufacturer’s instructions. cDNA was synthesized using M-MLV Reverse Transcriptase (Invitrogen) according to the manufacturer’s directions. qRT-PCR reactions were run in triplicate using SYBER Green master mix (BioRad) according to manufacturer’s protocol. qRT-PCR was performed using the CFX96 Real Time System (Bio-Rad). qRT-PCR primer sequences are provided in Supplemental Table . .. Immunoblotting Human fibroblasts were rinsed once with cold PBS and harvested with NP-40 lysis buffer, supplemented with 1X protease and phosphatase inhibitors (Thermo Fisher Scientific).

    Article Title: KRAS regulation by small non-coding RNAs and SNARE proteins
    Article Snippet: A fraction of each sample was set aside for total RNA isolation and another was resuspended 5 volumes CLIP lysis buffer (50 mM Tris, pH 7.5, 10% glycerol, 200 mM NaCl, 5 mM EDTA, 0.5% sarkosyl, 0.2% Tween and 0.1% Igepal). .. At this time both total RNA as well as CLIP fractions were purified using the miRNeasy Mini Kit (Qiagen).

    Article Title: Selective Histone Deacetylase 6 Inhibition Normalizes B Cell Activation and Germinal Center Formation in a Model of Systemic Lupus Erythematosus
    Article Snippet: .. mRNA-Isolation and Sequencing Total RNA was isolated from whole splenocytes using the miRNeasy Mini Kit (Qiagen, Germantown, MD, USA) per manufacturer's instructions. .. To remove residual amounts of DNA contamination in isolated RNA, on-column DNase digestion with RNase-Free DNase was performed.

    Size-exclusion Chromatography:

    Article Title: MicroRNA-448 modulates the progression of neuropathic pain by targeting sirtuin 1
    Article Snippet: RNA extraction and reverse-transcription quantitative polymerase chain reaction (RT-qPCR) Total RNA was isolated from tissues or cells using a miRNeasy mini kit (Qiagen) according to the manufacturer's protocol. .. The PCR conditions were as follows: 95°C for 5 min, followed by 40 cycles of 95°C for 30 sec, 60°C for 30 sec, and 72°C for 40 sec and then a final extension at 72°C for 5 min. mRNA levels were quantified using the 2−ΔΔCq method and normalized to the internal reference gene GAPDH or U6 ( ).

    Labeling:

    Article Title: MicroRNA-132-3p suppresses type I IFN response through targeting IRF1 to facilitate H1N1 influenza A virus infection
    Article Snippet: MicroRNA microarray analysis RNA was extracted from peripheral blood samples from three influenza patients and three healthy controls using an miRNeasy Mini Kit (QIAGEN GmbH, Hilden, Germany) and then the RNA concentration were analyzed with an NanoDrop ND-2000 spectrophotometry (Thermo Fisher Scientific, Inc., Waltham, MA, U.S.A.). .. About 1 μg of total RNA was used as the input for the labeling reaction and hybridized using the miRCURY Hy3/Hy5 Power Labeling kit and miRCURY™ LNA array (v.16.0; Exiqon A/S, Copenhagen, Denmark) according to the manufacturer’s protocol.

    Article Title: Dietary delphinidin inhibits human colorectal cancer metastasis associating with upregulation of miR-204-3p and suppression of the integrin/FAK axis
    Article Snippet: .. In brief, total RNA from DLD-1 cells was extracted using the miRNeasy mini kit (QIAGEN, Mainz, Germany) and labeled with Cy3 during reverse transcription to cDNA using an Agilent miRNA Labeling Kit (Agilent, UK) and Spike Kit (Agilent, UK). .. The labeled cDNAs were hybridized (in duplicate) to Human miRNA Microarray, Release 19.0, 8 × 60 K (v19) microarray slides (Agilent, UK) according to the microRNA Hybridization Kit protocol (Agilent, UK) and scanned using a Nimblegen MS200 array scanner.

    Purification:

    Article Title: Ube3a-ATS is an atypical RNA polymerase II transcript that represses the paternal expression of Ube3a
    Article Snippet: Total RNA was prepared with miRNeasy Mini Kit (Qiagen, Valencia, CA, USA). .. The mRNA was purified from 10 to 30 μg of total RNA with oligo(dT) beads supplied in Illumina mRNA-seq Sample Preparation Kit according to the manufacturer's instructions (Illumina, San Diego, CA, USA).

    Article Title: High-glucose-induced miR-214-3p inhibits BMSCs osteogenic differentiation in type 1 diabetes mellitus
    Article Snippet: After purification, 1 μg of total RNA was reverse transcribed into cDNA using a High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems). .. The miRNeasy Mini Kit (QIAGEN, USA) was used to extract miRNAs from bone specimens or BMSCs.

    Article Title: Whole Genome Expression Analyses of miRNAs and mRNAs Suggest the Involvement of miR-320a and miR-155-3p and their Targeted Genes in Lithium Response in Bipolar Disorder
    Article Snippet: Genome-Wide NGS Analysis of miRNAs Total RNA was extracted using the miRNeasy Mini Kit (QIAGEN GmbH-Hilden, Hilden, Germany). .. During library preparation, small RNAs having the 3′ hydroxyl end and the 5′-phosphate group produced by Dicer were selectively ligated to adapters, retrotranscribed, amplified and indexed, and finally purified to produce pure miRNA libraries.

    Article Title: KRAS regulation by small non-coding RNAs and SNARE proteins
    Article Snippet: .. At this time both total RNA as well as CLIP fractions were purified using the miRNeasy Mini Kit (Qiagen). .. Reverse transcription using the ISCRIPT cDNA synthesis kit (Biorad) with target specific priming for SNORD50A/B was used to generate cDNA, and qPCR was performed using SYBRgreen (ThermoFischer Scientific).

    Polymerase Chain Reaction:

    Article Title: MicroRNA-448 modulates the progression of neuropathic pain by targeting sirtuin 1
    Article Snippet: RNA extraction and reverse-transcription quantitative polymerase chain reaction (RT-qPCR) Total RNA was isolated from tissues or cells using a miRNeasy mini kit (Qiagen) according to the manufacturer's protocol. .. The PCR conditions were as follows: 95°C for 5 min, followed by 40 cycles of 95°C for 30 sec, 60°C for 30 sec, and 72°C for 40 sec and then a final extension at 72°C for 5 min. mRNA levels were quantified using the 2−ΔΔCq method and normalized to the internal reference gene GAPDH or U6 ( ).

    IA:

    Article Title: Whole Genome Expression Analyses of miRNAs and mRNAs Suggest the Involvement of miR-320a and miR-155-3p and their Targeted Genes in Lithium Response in Bipolar Disorder
    Article Snippet: Genome-Wide NGS Analysis of miRNAs Total RNA was extracted using the miRNeasy Mini Kit (QIAGEN GmbH-Hilden, Hilden, Germany). .. Quantification and quality evaluation of miRNAs was performed using the Small RNA Analysis Kit (DNF-470) adjusted for the Fragment Analyzer™ Automated CE System (Advanced Analytical Technologies, Ankeny, IA, USA) and the Qubit RNA BR (Broad-Range) Assay Kit for Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA).

    Software:

    Article Title: MicroRNA-132-3p suppresses type I IFN response through targeting IRF1 to facilitate H1N1 influenza A virus infection
    Article Snippet: MicroRNA microarray analysis RNA was extracted from peripheral blood samples from three influenza patients and three healthy controls using an miRNeasy Mini Kit (QIAGEN GmbH, Hilden, Germany) and then the RNA concentration were analyzed with an NanoDrop ND-2000 spectrophotometry (Thermo Fisher Scientific, Inc., Waltham, MA, U.S.A.). .. Data were analyzed using the ImaGene® 9 (miRCURY LNA™ microRNA Array Analysis Software, Exiqon).

    Real-time Polymerase Chain Reaction:

    Article Title: Ube3a-ATS is an atypical RNA polymerase II transcript that represses the paternal expression of Ube3a
    Article Snippet: Total RNA was prepared with miRNeasy Mini Kit (Qiagen, Valencia, CA, USA). .. The cDNA was then generated using 0.2–1 μg of total RNA with SuperScript III First-Strand Synthesis System (Invitrogen), and q-PCR was performed using Applied Biosystems StepOnePlus Real-Time PCR System and SYBR Green Master Mix (Applied Biosystems, Carlsbad, CA, USA).

    Article Title: High-glucose-induced miR-214-3p inhibits BMSCs osteogenic differentiation in type 1 diabetes mellitus
    Article Snippet: Paragraph title: Quantitative real-time PCR ... The miRNeasy Mini Kit (QIAGEN, USA) was used to extract miRNAs from bone specimens or BMSCs.

    Article Title: MicroRNA-448 modulates the progression of neuropathic pain by targeting sirtuin 1
    Article Snippet: .. RNA extraction and reverse-transcription quantitative polymerase chain reaction (RT-qPCR) Total RNA was isolated from tissues or cells using a miRNeasy mini kit (Qiagen) according to the manufacturer's protocol. .. RT was performed using TaqMan miRNA reverse-transcription kit (Applied Biosystems; Thermo Fisher Scientific, Inc.) according to the manufacturer's protocol. qPCR was conducted to detect the expression levels of miR-448 and SIRT1 using a SYBR Premix Ex Taq™ II commercial kit (Takara Biotechnology Co. Ltd.) and the Applied Biosystems 7900 Real-Time PCR System (Applied Biosystems; Thermo Fisher Scientific, Inc.) according to the manufacturer's protocol.

    Article Title: p53 Represses the Oncogenic Sno-MiR-28 Derived from a SnoRNA
    Article Snippet: Paragraph title: RNA extraction and Real-time PCR ... Small RNAs (including miRNAs and sno-miRNAs) were extracted using the miRNeasy mini kit (Qiagen) following the manufacturer’s instructions.

    Article Title: KRAS regulation by small non-coding RNAs and SNARE proteins
    Article Snippet: At this time both total RNA as well as CLIP fractions were purified using the miRNeasy Mini Kit (Qiagen). .. Reverse transcription using the ISCRIPT cDNA synthesis kit (Biorad) with target specific priming for SNORD50A/B was used to generate cDNA, and qPCR was performed using SYBRgreen (ThermoFischer Scientific).

    Article Title: Expression and significance of miR - 20b in retinal photoreceptor cells exposed to PCB1254
    Article Snippet: Paragraph title: Real-time quantitative polymerase chain reaction (RT-qPCR) assay ... For miR-20b, miRNeasy Mini Kit (Qiagen Inc., Valencia, USA) was used for RNA reverse transcription.

    RNA Extraction:

    Article Title: LATS1 but not LATS2 represses autophagy by a kinase-independent scaffold function
    Article Snippet: .. RNA extraction and sequencing analysis RNA from siRNA-transfected and Srf-treated cells was extracted in biological triplicates using miRNeasy Mini kit (Qiagen) according to the manufacturer’s instructions. ..

    Article Title: Comparison of different extraction techniques to profile microRNAs from human sera and peripheral blood mononuclear cells
    Article Snippet: To investigate whether these observations were limited to the MN kit or whether RNA extraction with the Qiagen kit could introduce similar bias. .. Total RNAs were extracted from 1×106 or 3×106 PBMCs using the Qiagen miRNeasy mini kit and further profiled with TLDA cards.

    Article Title: MicroRNA-448 modulates the progression of neuropathic pain by targeting sirtuin 1
    Article Snippet: .. RNA extraction and reverse-transcription quantitative polymerase chain reaction (RT-qPCR) Total RNA was isolated from tissues or cells using a miRNeasy mini kit (Qiagen) according to the manufacturer's protocol. .. RT was performed using TaqMan miRNA reverse-transcription kit (Applied Biosystems; Thermo Fisher Scientific, Inc.) according to the manufacturer's protocol. qPCR was conducted to detect the expression levels of miR-448 and SIRT1 using a SYBR Premix Ex Taq™ II commercial kit (Takara Biotechnology Co. Ltd.) and the Applied Biosystems 7900 Real-Time PCR System (Applied Biosystems; Thermo Fisher Scientific, Inc.) according to the manufacturer's protocol.

    Article Title: p53 Represses the Oncogenic Sno-MiR-28 Derived from a SnoRNA
    Article Snippet: Paragraph title: RNA extraction and Real-time PCR ... Small RNAs (including miRNAs and sno-miRNAs) were extracted using the miRNeasy mini kit (Qiagen) following the manufacturer’s instructions.

    Sample Prep:

    Article Title: Ube3a-ATS is an atypical RNA polymerase II transcript that represses the paternal expression of Ube3a
    Article Snippet: Total RNA was prepared with miRNeasy Mini Kit (Qiagen, Valencia, CA, USA). .. The mRNA was purified from 10 to 30 μg of total RNA with oligo(dT) beads supplied in Illumina mRNA-seq Sample Preparation Kit according to the manufacturer's instructions (Illumina, San Diego, CA, USA).

    Article Title: LATS1 but not LATS2 represses autophagy by a kinase-independent scaffold function
    Article Snippet: RNA extraction and sequencing analysis RNA from siRNA-transfected and Srf-treated cells was extracted in biological triplicates using miRNeasy Mini kit (Qiagen) according to the manufacturer’s instructions. .. A total of 200 ng of RNA was utilized for library preparation with the TruSeq stranded total RNA LT sample prep Kit (Illumina).

    Next-Generation Sequencing:

    Article Title: Whole Genome Expression Analyses of miRNAs and mRNAs Suggest the Involvement of miR-320a and miR-155-3p and their Targeted Genes in Lithium Response in Bipolar Disorder
    Article Snippet: .. Genome-Wide NGS Analysis of miRNAs Total RNA was extracted using the miRNeasy Mini Kit (QIAGEN GmbH-Hilden, Hilden, Germany). .. Quantification and quality evaluation of miRNAs was performed using the Small RNA Analysis Kit (DNF-470) adjusted for the Fragment Analyzer™ Automated CE System (Advanced Analytical Technologies, Ankeny, IA, USA) and the Qubit RNA BR (Broad-Range) Assay Kit for Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA).

    Incubation:

    Article Title: KRAS regulation by small non-coding RNAs and SNARE proteins
    Article Snippet: The fraction set aside for CLIP was incubated overnight at 4 C with end over end turning with Protein G beads (ThermoFischer Scientific) precoupled to an anti-panras C4 antibody. .. At this time both total RNA as well as CLIP fractions were purified using the miRNeasy Mini Kit (Qiagen).

    Spectrophotometry:

    Article Title: MicroRNA-132-3p suppresses type I IFN response through targeting IRF1 to facilitate H1N1 influenza A virus infection
    Article Snippet: .. MicroRNA microarray analysis RNA was extracted from peripheral blood samples from three influenza patients and three healthy controls using an miRNeasy Mini Kit (QIAGEN GmbH, Hilden, Germany) and then the RNA concentration were analyzed with an NanoDrop ND-2000 spectrophotometry (Thermo Fisher Scientific, Inc., Waltham, MA, U.S.A.). .. About 1 μg of total RNA was used as the input for the labeling reaction and hybridized using the miRCURY Hy3/Hy5 Power Labeling kit and miRCURY™ LNA array (v.16.0; Exiqon A/S, Copenhagen, Denmark) according to the manufacturer’s protocol.

    Article Title: Circular RNAs in Clear Cell Renal Cell Carcinoma: Their Microarray-Based Identification, Analytical Validation, and Potential Use in a Clinico-Genomic Model to Improve Prognostic Accuracy
    Article Snippet: .. The isolation procedure from preserved tissue specimens using the miRNeasy Mini Kit (Qiagen) including an on-column DNA digestion step according to the producer's instructions, the spectrophotometric quantification (NanoDrop 1000 Spectrophotometer; NanoDrop Technologies, Wilmington, DE, USA), and the quality assessment of the total RNA samples (ratio of the absorbance at 260 nm to that at 280 nm and RNA integrity number on a Bioanalyzer 21000 (Agilent Technologies, Santa Clara, CA, USA) were detailed described in our previous publications [ , , ]. .. The median ratio of 260 nm to 280 nm of the isolated RNA samples was 2.04 (95% CI, 2.03 to 2.04) and the median RNA integrity number was 7.70 (95% CI, 7.52 to 7.88).

    Produced:

    Article Title: Whole Genome Expression Analyses of miRNAs and mRNAs Suggest the Involvement of miR-320a and miR-155-3p and their Targeted Genes in Lithium Response in Bipolar Disorder
    Article Snippet: Genome-Wide NGS Analysis of miRNAs Total RNA was extracted using the miRNeasy Mini Kit (QIAGEN GmbH-Hilden, Hilden, Germany). .. During library preparation, small RNAs having the 3′ hydroxyl end and the 5′-phosphate group produced by Dicer were selectively ligated to adapters, retrotranscribed, amplified and indexed, and finally purified to produce pure miRNA libraries.

    Immunoprecipitation:

    Article Title: KRAS regulation by small non-coding RNAs and SNARE proteins
    Article Snippet: Paragraph title: Crosslinking followed by Immunoprecipitation ... At this time both total RNA as well as CLIP fractions were purified using the miRNeasy Mini Kit (Qiagen).

    Fractionation:

    Article Title: KRAS regulation by small non-coding RNAs and SNARE proteins
    Article Snippet: Crosslinking followed by Immunoprecipitation H23 and a SNORD50A/B KO subclone of the parental line were subjected to 0.3 J/cm2 UV-C crosslinking on ice then subjected to fractionation using the Plasma Membrane Isolation Kit (Abcam) according to manufacturer’s instructions. .. At this time both total RNA as well as CLIP fractions were purified using the miRNeasy Mini Kit (Qiagen).

    Lysis:

    Article Title: KRAS regulation by small non-coding RNAs and SNARE proteins
    Article Snippet: A fraction of each sample was set aside for total RNA isolation and another was resuspended 5 volumes CLIP lysis buffer (50 mM Tris, pH 7.5, 10% glycerol, 200 mM NaCl, 5 mM EDTA, 0.5% sarkosyl, 0.2% Tween and 0.1% Igepal). .. At this time both total RNA as well as CLIP fractions were purified using the miRNeasy Mini Kit (Qiagen).

    Cross-linking Immunoprecipitation:

    Article Title: KRAS regulation by small non-coding RNAs and SNARE proteins
    Article Snippet: .. At this time both total RNA as well as CLIP fractions were purified using the miRNeasy Mini Kit (Qiagen). .. Reverse transcription using the ISCRIPT cDNA synthesis kit (Biorad) with target specific priming for SNORD50A/B was used to generate cDNA, and qPCR was performed using SYBRgreen (ThermoFischer Scientific).

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    Qiagen mirneasy mini kit
    Mirneasy Mini Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 967 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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