2100bioanalyzer high sensitivity dna kit agilent technologies  (Agilent technologies)

 
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    Agilent technologies 2100bioanalyzer high sensitivity dna kit agilent technologies
    2100bioanalyzer High Sensitivity Dna Kit Agilent Technologies, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/2100bioanalyzer high sensitivity dna kit agilent technologies/product/Agilent technologies
    Average 79 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    2100bioanalyzer high sensitivity dna kit agilent technologies - by Bioz Stars, 2020-02
    79/100 stars

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    Centrifugation:

    Article Title: Persistent viremia by a novel parvovirus in a slow loris (Nycticebus coucang) with diffuse histiocytic sarcoma
    Article Snippet: After a centrifugation and DNase treatment to remove both intact cells and host DNA derived from broken cells, viral nucleic acids were isolated from 100 μl of serum with the QIAmp DNA mini kit (Qiagen). .. The library was pooled with other samples, the average size of the whole library was estimated with Agilent 2100Bioanalyzer (high sensitivity DNA kit, Agilent Technologies) and the final concentration (copies/μl) was calculated using the KAPA Library Quantification kit (KAPA Biosystems).

    Amplification:

    Article Title: Persistent viremia by a novel parvovirus in a slow loris (Nycticebus coucang) with diffuse histiocytic sarcoma
    Article Snippet: The amplified library was subjected to 2 consecutive purification rounds with Agencourt AMPure XP beads to completely remove excess primers and short fragments and DNA concentration was measured on a Qubit Fluorometer (Quant-it ds DNA HS kit, Invitrogen). .. The library was pooled with other samples, the average size of the whole library was estimated with Agilent 2100Bioanalyzer (high sensitivity DNA kit, Agilent Technologies) and the final concentration (copies/μl) was calculated using the KAPA Library Quantification kit (KAPA Biosystems).

    Ligation:

    Article Title: Persistent viremia by a novel parvovirus in a slow loris (Nycticebus coucang) with diffuse histiocytic sarcoma
    Article Snippet: Subsequent to the ligation of adaptors containing specific Roche-454 primer binding sequences, an amplicon size-selection was performed to prevent the amplification of DNA fragments smaller than 200 bp using Agencourt AMPure XP beads (Beckman Coulter), followed by 30 cycles of PCR amplification. .. The library was pooled with other samples, the average size of the whole library was estimated with Agilent 2100Bioanalyzer (high sensitivity DNA kit, Agilent Technologies) and the final concentration (copies/μl) was calculated using the KAPA Library Quantification kit (KAPA Biosystems).

    Isolation:

    Article Title: Persistent viremia by a novel parvovirus in a slow loris (Nycticebus coucang) with diffuse histiocytic sarcoma
    Article Snippet: After a centrifugation and DNase treatment to remove both intact cells and host DNA derived from broken cells, viral nucleic acids were isolated from 100 μl of serum with the QIAmp DNA mini kit (Qiagen). .. The library was pooled with other samples, the average size of the whole library was estimated with Agilent 2100Bioanalyzer (high sensitivity DNA kit, Agilent Technologies) and the final concentration (copies/μl) was calculated using the KAPA Library Quantification kit (KAPA Biosystems).

    Polymerase Chain Reaction:

    Article Title: Persistent viremia by a novel parvovirus in a slow loris (Nycticebus coucang) with diffuse histiocytic sarcoma
    Article Snippet: Subsequent to the ligation of adaptors containing specific Roche-454 primer binding sequences, an amplicon size-selection was performed to prevent the amplification of DNA fragments smaller than 200 bp using Agencourt AMPure XP beads (Beckman Coulter), followed by 30 cycles of PCR amplification. .. The library was pooled with other samples, the average size of the whole library was estimated with Agilent 2100Bioanalyzer (high sensitivity DNA kit, Agilent Technologies) and the final concentration (copies/μl) was calculated using the KAPA Library Quantification kit (KAPA Biosystems).

    Purification:

    Article Title: Persistent viremia by a novel parvovirus in a slow loris (Nycticebus coucang) with diffuse histiocytic sarcoma
    Article Snippet: The amplified library was subjected to 2 consecutive purification rounds with Agencourt AMPure XP beads to completely remove excess primers and short fragments and DNA concentration was measured on a Qubit Fluorometer (Quant-it ds DNA HS kit, Invitrogen). .. The library was pooled with other samples, the average size of the whole library was estimated with Agilent 2100Bioanalyzer (high sensitivity DNA kit, Agilent Technologies) and the final concentration (copies/μl) was calculated using the KAPA Library Quantification kit (KAPA Biosystems).

    Concentration Assay:

    Article Title: Persistent viremia by a novel parvovirus in a slow loris (Nycticebus coucang) with diffuse histiocytic sarcoma
    Article Snippet: .. The library was pooled with other samples, the average size of the whole library was estimated with Agilent 2100Bioanalyzer (high sensitivity DNA kit, Agilent Technologies) and the final concentration (copies/μl) was calculated using the KAPA Library Quantification kit (KAPA Biosystems). .. Samples were then diluted to a final concentration of 1 million copies/μl and used as input for the emulsion PCR (LIB-A emPCR kit, Roche) and 454 pyrosequencing (Roche).

    Sequencing:

    Article Title: Persistent viremia by a novel parvovirus in a slow loris (Nycticebus coucang) with diffuse histiocytic sarcoma
    Article Snippet: Virus discovery Sequence independent virus discovery was performed on a serum sample collected from Buddha at necropsy (2008) with the previously described VIDISCA-454 procedure (De Vries et al., ) with minor modifications. .. The library was pooled with other samples, the average size of the whole library was estimated with Agilent 2100Bioanalyzer (high sensitivity DNA kit, Agilent Technologies) and the final concentration (copies/μl) was calculated using the KAPA Library Quantification kit (KAPA Biosystems).

    Binding Assay:

    Article Title: Persistent viremia by a novel parvovirus in a slow loris (Nycticebus coucang) with diffuse histiocytic sarcoma
    Article Snippet: Subsequent to the ligation of adaptors containing specific Roche-454 primer binding sequences, an amplicon size-selection was performed to prevent the amplification of DNA fragments smaller than 200 bp using Agencourt AMPure XP beads (Beckman Coulter), followed by 30 cycles of PCR amplification. .. The library was pooled with other samples, the average size of the whole library was estimated with Agilent 2100Bioanalyzer (high sensitivity DNA kit, Agilent Technologies) and the final concentration (copies/μl) was calculated using the KAPA Library Quantification kit (KAPA Biosystems).

    Derivative Assay:

    Article Title: Persistent viremia by a novel parvovirus in a slow loris (Nycticebus coucang) with diffuse histiocytic sarcoma
    Article Snippet: After a centrifugation and DNase treatment to remove both intact cells and host DNA derived from broken cells, viral nucleic acids were isolated from 100 μl of serum with the QIAmp DNA mini kit (Qiagen). .. The library was pooled with other samples, the average size of the whole library was estimated with Agilent 2100Bioanalyzer (high sensitivity DNA kit, Agilent Technologies) and the final concentration (copies/μl) was calculated using the KAPA Library Quantification kit (KAPA Biosystems).

    Software:

    Article Title: Persistent viremia by a novel parvovirus in a slow loris (Nycticebus coucang) with diffuse histiocytic sarcoma
    Article Snippet: The library was pooled with other samples, the average size of the whole library was estimated with Agilent 2100Bioanalyzer (high sensitivity DNA kit, Agilent Technologies) and the final concentration (copies/μl) was calculated using the KAPA Library Quantification kit (KAPA Biosystems). .. After sequencing, primer sequences were trimmed from every read and sequences were assembled with CodonCode Aligner software, version 3.5.6.

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  • 79
    Agilent technologies 2100bioanalyzer high sensitivity dna kit agilent technologies
    2100bioanalyzer High Sensitivity Dna Kit Agilent Technologies, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/2100bioanalyzer high sensitivity dna kit agilent technologies/product/Agilent technologies
    Average 79 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    2100bioanalyzer high sensitivity dna kit agilent technologies - by Bioz Stars, 2020-02
    79/100 stars
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