Structured Review

Agilent technologies 2100 bioanalyzer
Postfixation processing of an RNA 50mer with excess formaldehyde removed. Gel-simulated image (Agilent <t>2100</t> <t>Bioanalyzer)</t> of the RNA 50mer fixed in 5% buffered formaldehyde (pH 7.4) for 2 hours. A: Lane L: RNA ladder. Lane 1: Non–formaldehyde-treated
2100 Bioanalyzer, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 148 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "The Effect of Formaldehyde Fixation on RNA"

Article Title: The Effect of Formaldehyde Fixation on RNA

Journal: The Journal of Molecular Diagnostics : JMD

doi: 10.1016/j.jmoldx.2011.01.010

Postfixation processing of an RNA 50mer with excess formaldehyde removed. Gel-simulated image (Agilent 2100 Bioanalyzer) of the RNA 50mer fixed in 5% buffered formaldehyde (pH 7.4) for 2 hours. A: Lane L: RNA ladder. Lane 1: Non–formaldehyde-treated
Figure Legend Snippet: Postfixation processing of an RNA 50mer with excess formaldehyde removed. Gel-simulated image (Agilent 2100 Bioanalyzer) of the RNA 50mer fixed in 5% buffered formaldehyde (pH 7.4) for 2 hours. A: Lane L: RNA ladder. Lane 1: Non–formaldehyde-treated

Techniques Used:

Effects of fixation and postfixation processing on total cellular RNA: gel-simulated image (Agilent 2100 Bioanalyzer) of total cellular RNA fixed in 5% buffered formaldehyde (pH 7.4) for 2 hours. Lane L: RNA ladder. Lane 1: Native non–formaldehyde-treated
Figure Legend Snippet: Effects of fixation and postfixation processing on total cellular RNA: gel-simulated image (Agilent 2100 Bioanalyzer) of total cellular RNA fixed in 5% buffered formaldehyde (pH 7.4) for 2 hours. Lane L: RNA ladder. Lane 1: Native non–formaldehyde-treated

Techniques Used:

Effects of buffer composition on postfixation processing of an RNA 50mer: gel-simulated image (Agilent 2100 Bioanalyzer). Lane L: RNA ladder. Lane 1: Native RNA 50mer. Lane 2: RNA 50mer fixed in 5% buffered formaldehyde (pH 7.4) for 2 hours. Excess formaldehyde
Figure Legend Snippet: Effects of buffer composition on postfixation processing of an RNA 50mer: gel-simulated image (Agilent 2100 Bioanalyzer). Lane L: RNA ladder. Lane 1: Native RNA 50mer. Lane 2: RNA 50mer fixed in 5% buffered formaldehyde (pH 7.4) for 2 hours. Excess formaldehyde

Techniques Used:

Postformaldehyde fixation processing of an RNA 50mer. Gel-simulated image (Agilent 2100 Bioanalyzer) of the RNA 50mer fixed in 5% buffered formaldehyde (pH 7.4) for 2 hours and then heated at 70°C for 30 minutes, where indicated, in the following
Figure Legend Snippet: Postformaldehyde fixation processing of an RNA 50mer. Gel-simulated image (Agilent 2100 Bioanalyzer) of the RNA 50mer fixed in 5% buffered formaldehyde (pH 7.4) for 2 hours and then heated at 70°C for 30 minutes, where indicated, in the following

Techniques Used:

Effects of fixation and postfixation processing on total HeLa RNA. Electropherograms (Agilent 2100 Bioanalyzer) of total cellular RNA fixed in 5% buffered formaldehyde (pH 7.4) for 2 hours. FU denotes fluorescence units. A: Non–formaldehyde-treated
Figure Legend Snippet: Effects of fixation and postfixation processing on total HeLa RNA. Electropherograms (Agilent 2100 Bioanalyzer) of total cellular RNA fixed in 5% buffered formaldehyde (pH 7.4) for 2 hours. FU denotes fluorescence units. A: Non–formaldehyde-treated

Techniques Used: Fluorescence

2) Product Images from "High quality RNA extraction from Maqui berry for its application in next-generation sequencing"

Article Title: High quality RNA extraction from Maqui berry for its application in next-generation sequencing

Journal: SpringerPlus

doi: 10.1186/s40064-016-2906-x

Electrophoretogram of sequencing libraries. The graph shows the length distribution curves of sequencing libraries obtained with Illumina TruSeqTM RNA sample preparation kit (Low-Throughput protocol) according to manufacturer’s protocol. The length of DNA fragments was between 200 and 700 pb. Curves were generated on a 2100 bioanalyzer using DNA 1000 chip (Agilent Technologies). For each sample three biological replicates were performed
Figure Legend Snippet: Electrophoretogram of sequencing libraries. The graph shows the length distribution curves of sequencing libraries obtained with Illumina TruSeqTM RNA sample preparation kit (Low-Throughput protocol) according to manufacturer’s protocol. The length of DNA fragments was between 200 and 700 pb. Curves were generated on a 2100 bioanalyzer using DNA 1000 chip (Agilent Technologies). For each sample three biological replicates were performed

Techniques Used: Sequencing, Sample Prep, Generated, Chromatin Immunoprecipitation

Electrophoretogram of total RNA obtained with our new method. The 18S and 28S rRNA 25 regions are shown. RNA concentrations and RIN values are shown below. a green fruit. b red fruit. c blue fruit. RNA was analyzed with the Agilent RNA 6000 Nano Assay in a 2100 bioanalyzer (Agilent Technologies). Note that the output from our instrument is completely opposite from the English convention, therefore, it uses commas as an indicator of decimals, and periods to denote thousands. It is shown one result of the three RNA extraction obtained from the same tissue
Figure Legend Snippet: Electrophoretogram of total RNA obtained with our new method. The 18S and 28S rRNA 25 regions are shown. RNA concentrations and RIN values are shown below. a green fruit. b red fruit. c blue fruit. RNA was analyzed with the Agilent RNA 6000 Nano Assay in a 2100 bioanalyzer (Agilent Technologies). Note that the output from our instrument is completely opposite from the English convention, therefore, it uses commas as an indicator of decimals, and periods to denote thousands. It is shown one result of the three RNA extraction obtained from the same tissue

Techniques Used: RNA Extraction

3) Product Images from "The characterization of four gene expression analysis in circulating tumor cells made by Multiplex-PCR from the AdnaTest kit on the lab-on-a-chip Agilent DNA 1000 platform"

Article Title: The characterization of four gene expression analysis in circulating tumor cells made by Multiplex-PCR from the AdnaTest kit on the lab-on-a-chip Agilent DNA 1000 platform

Journal: Biochemia Medica

doi: 10.11613/BM.2016.011

Baseline record of the whole Agilent DNA 1000 chip analysis on 2100 Bioanalyzer. Ladder well is measured first followed by 12 wells filled with the same positive control. The fluorescence intensity is decreasing during the measurement. L - ladder, 1C +…12C + - 12 measurements of one positive control.
Figure Legend Snippet: Baseline record of the whole Agilent DNA 1000 chip analysis on 2100 Bioanalyzer. Ladder well is measured first followed by 12 wells filled with the same positive control. The fluorescence intensity is decreasing during the measurement. L - ladder, 1C +…12C + - 12 measurements of one positive control.

Techniques Used: Chromatin Immunoprecipitation, Positive Control, Fluorescence

A scheme of the experiments investigating the characteristics of the concentration determination by the Agilent 1000 DNA kit on the 2100 Bioanalyzer. a) Repeatability: One positive control stored at -20°C after Multi-PCR was slowly thawed, vortexes and briefly spun down. One µL was put into each of the twelve wells on one chip. b) Robustness: 1) Sample volume changed: 0.8 µL; 0.9 µL; 1.0 µL; 1.1 µL; 1.1 µL; 1.0 µL; 0.9 µL; 0.8 µL of one positive control was put into wells number 1, 2, 3, 4, 5, 6, 7, 8 on one chip instead of the standard 1 µL respectively. The measurement was repeated three times on different chips. 2) Marker mix volume changed: 4.5 µL; 4.8 µL; 5.0 µL and 5.2 µL of the well-mixed Marker mix put into different wells on one chip with 1 µL of positive control. The measurement was repeated three times on different chips. c) Inter-Multi PCR repeatability: Multi-PCR with one positive control from AdnaTest ProstateCancerDetect kit was run in three separate test tubes in the same thermo cycler run using the same master mix. The concentration of each PCR product was measured in three different wells on one chip. d) Inter-Multi PCR reproducibility: One positive control from the AdnaGen ProstateCancerDetect kit was used in six different Multi-PCRs run, measured on six different chips. e) Repeatability: Three different samples (frozen after Multi-PCR) were measured in triplets on one chip. f) Reproducibility: Twelve cDNA samples (obtained after RT) frozen for 10 months were thawed. The Multi-PCR was repeated. New PCR products (1B-12 after 10 months) were measured on one chip. Frozen mixtures of these samples generated by the first Multi-PCR were re measured on the second chip (1A-12 after 10 months) and compared with the previous results (1A-12).
Figure Legend Snippet: A scheme of the experiments investigating the characteristics of the concentration determination by the Agilent 1000 DNA kit on the 2100 Bioanalyzer. a) Repeatability: One positive control stored at -20°C after Multi-PCR was slowly thawed, vortexes and briefly spun down. One µL was put into each of the twelve wells on one chip. b) Robustness: 1) Sample volume changed: 0.8 µL; 0.9 µL; 1.0 µL; 1.1 µL; 1.1 µL; 1.0 µL; 0.9 µL; 0.8 µL of one positive control was put into wells number 1, 2, 3, 4, 5, 6, 7, 8 on one chip instead of the standard 1 µL respectively. The measurement was repeated three times on different chips. 2) Marker mix volume changed: 4.5 µL; 4.8 µL; 5.0 µL and 5.2 µL of the well-mixed Marker mix put into different wells on one chip with 1 µL of positive control. The measurement was repeated three times on different chips. c) Inter-Multi PCR repeatability: Multi-PCR with one positive control from AdnaTest ProstateCancerDetect kit was run in three separate test tubes in the same thermo cycler run using the same master mix. The concentration of each PCR product was measured in three different wells on one chip. d) Inter-Multi PCR reproducibility: One positive control from the AdnaGen ProstateCancerDetect kit was used in six different Multi-PCRs run, measured on six different chips. e) Repeatability: Three different samples (frozen after Multi-PCR) were measured in triplets on one chip. f) Reproducibility: Twelve cDNA samples (obtained after RT) frozen for 10 months were thawed. The Multi-PCR was repeated. New PCR products (1B-12 after 10 months) were measured on one chip. Frozen mixtures of these samples generated by the first Multi-PCR were re measured on the second chip (1A-12 after 10 months) and compared with the previous results (1A-12).

Techniques Used: Concentration Assay, Positive Control, Polymerase Chain Reaction, Chromatin Immunoprecipitation, Marker, Generated

4) Product Images from "Generating Exome Enriched Sequencing Libraries from Formalin-Fixed, Paraffin-Embedded Tissue DNA for Next Generation Sequencing"

Article Title: Generating Exome Enriched Sequencing Libraries from Formalin-Fixed, Paraffin-Embedded Tissue DNA for Next Generation Sequencing

Journal: Current protocols in human genetics

doi: 10.1002/cphg.27

DNA fragmentation quality check. 50ng of FFPE derived DNA was sheared using the Covaris E220 instrument. Following clean up each sample was diluted 1:5 and run on a 2100 BioAnalyzer High Sensitivity DNA chip to check the DNA fragment size distribution and sample concentration. A broad distribution of sizes should be expected between 100-2000bp is typical, with the majority of the fragments in the 200-800bp range.
Figure Legend Snippet: DNA fragmentation quality check. 50ng of FFPE derived DNA was sheared using the Covaris E220 instrument. Following clean up each sample was diluted 1:5 and run on a 2100 BioAnalyzer High Sensitivity DNA chip to check the DNA fragment size distribution and sample concentration. A broad distribution of sizes should be expected between 100-2000bp is typical, with the majority of the fragments in the 200-800bp range.

Techniques Used: Formalin-fixed Paraffin-Embedded, Derivative Assay, Chromatin Immunoprecipitation, Concentration Assay

Pre-Capture amplification quality check. Adapter ligated libraries were amplified prior to hybridization capture. Following clean up each sample was diluted 1:100 and run on a 2100 BioAnalyzer High Sensitivity DNA chip to confirm library fragment size and concentration. A focused size distribution between 200-800bp is typical. Quality of FFPE samples may affect how this distribution appears (average size between 250-550bp). Fragment size includes the added length of bases (123bp) from the ligated adapters.
Figure Legend Snippet: Pre-Capture amplification quality check. Adapter ligated libraries were amplified prior to hybridization capture. Following clean up each sample was diluted 1:100 and run on a 2100 BioAnalyzer High Sensitivity DNA chip to confirm library fragment size and concentration. A focused size distribution between 200-800bp is typical. Quality of FFPE samples may affect how this distribution appears (average size between 250-550bp). Fragment size includes the added length of bases (123bp) from the ligated adapters.

Techniques Used: Amplification, Hybridization, Chromatin Immunoprecipitation, Concentration Assay, Formalin-fixed Paraffin-Embedded

5) Product Images from "Generating Exome Enriched Sequencing Libraries from Formalin-Fixed, Paraffin-Embedded Tissue DNA for Next Generation Sequencing"

Article Title: Generating Exome Enriched Sequencing Libraries from Formalin-Fixed, Paraffin-Embedded Tissue DNA for Next Generation Sequencing

Journal: Current protocols in human genetics

doi: 10.1002/cphg.27

DNA fragmentation quality check. 50ng of FFPE derived DNA was sheared using the Covaris E220 instrument. Following clean up each sample was diluted 1:5 and run on a 2100 BioAnalyzer High Sensitivity DNA chip to check the DNA fragment size distribution and sample concentration. A broad distribution of sizes should be expected between 100-2000bp is typical, with the majority of the fragments in the 200-800bp range.
Figure Legend Snippet: DNA fragmentation quality check. 50ng of FFPE derived DNA was sheared using the Covaris E220 instrument. Following clean up each sample was diluted 1:5 and run on a 2100 BioAnalyzer High Sensitivity DNA chip to check the DNA fragment size distribution and sample concentration. A broad distribution of sizes should be expected between 100-2000bp is typical, with the majority of the fragments in the 200-800bp range.

Techniques Used: Formalin-fixed Paraffin-Embedded, Derivative Assay, Chromatin Immunoprecipitation, Concentration Assay

Pre-Capture amplification quality check. Adapter ligated libraries were amplified prior to hybridization capture. Following clean up each sample was diluted 1:100 and run on a 2100 BioAnalyzer High Sensitivity DNA chip to confirm library fragment size and concentration. A focused size distribution between 200-800bp is typical. Quality of FFPE samples may affect how this distribution appears (average size between 250-550bp). Fragment size includes the added length of bases (123bp) from the ligated adapters.
Figure Legend Snippet: Pre-Capture amplification quality check. Adapter ligated libraries were amplified prior to hybridization capture. Following clean up each sample was diluted 1:100 and run on a 2100 BioAnalyzer High Sensitivity DNA chip to confirm library fragment size and concentration. A focused size distribution between 200-800bp is typical. Quality of FFPE samples may affect how this distribution appears (average size between 250-550bp). Fragment size includes the added length of bases (123bp) from the ligated adapters.

Techniques Used: Amplification, Hybridization, Chromatin Immunoprecipitation, Concentration Assay, Formalin-fixed Paraffin-Embedded

6) Product Images from "Effects of Acute Aerobic Exercise on Rats Serum Extracellular Vesicles Diameter, Concentration and Small RNAs Content"

Article Title: Effects of Acute Aerobic Exercise on Rats Serum Extracellular Vesicles Diameter, Concentration and Small RNAs Content

Journal: Frontiers in Physiology

doi: 10.3389/fphys.2018.00532

Acute aerobic exercise decreases rat serum EVs modal diameter and increases serum EVs concentration. (A) Workflow representing the acute exercise protocol used to provide aerobic exercise for Wistar rats running on a treadmill. (B) Immunoelectron micrograph of rat serum EVs purified by Exoquick. Black dots are 10 nm gold particle linked to the exosome marker CD63. (C) Tunable resistive pulse sensing (TRPS) analysis showed a slightly reduction in EVs modal diameter in high intensity exercised group compared to non-exercised ( p = 0.057) and moderate intensity exercised groups ( p = 0.028). The median groups for EVs modal diameter range from 88 nm in non-exercised, through 87 nm in low intensity, 91.5 nm in moderate intensity and 85 nm in high intensity exercised group (D) . The concentration of EVs purified from rat’s serum after exercise measured by TRPS. There is a statistical increase in median EVs concentration after exercise, which range from 1.1 × 10 9 in non-exercised group to 3 × 10 9 in low intensity exercised group ( p = 0.014), 2.5 × 10 9 in moderate intensity exercised group ( p = 0.021) and 3 × 10 9 in high intensity exercised group ( p = 0.02). (E) The median concentration of rat serum EVs proteins. Proteins were purified from EVs and quantified. There is a statistical significance increase in EVs protein concentration median after exercise ranging from 0.935 mg.mL -1 in non-exercised group to 4.33 mg.mL -1 in low intensity exercised group ( p = 0.014), 4.31 mg.mL -1 in moderate intensity exercised group ( p = 0.014) and 4.31 mg.mL -1 in high intensity exercised group ( p = 0.014). (F) Total rat serum protein profile after Exoquick purification analyzed by SDS-PAGE 12%. Exoquick fraction contains EVs proteins while supernatant fraction contains free proteins that were not precipitated by Exoquick. (G) Western blotting of EVs proteins shows an increase in the exosome marker CD63 while increasing the intensity of exercise. ApoA-IV protein levels remained stable regardless of exercise intensity. Calnexin was used to show the absence of endoplasmic reticulum proteins in purified EVs. (H) The concentration of EVs small RNAs from rat’s serum. Small RNAs were purified, quantified and characterized by 2100 Bioanalyzer (Agilent) using a small RNA chip. There is no statistical difference between group medians.
Figure Legend Snippet: Acute aerobic exercise decreases rat serum EVs modal diameter and increases serum EVs concentration. (A) Workflow representing the acute exercise protocol used to provide aerobic exercise for Wistar rats running on a treadmill. (B) Immunoelectron micrograph of rat serum EVs purified by Exoquick. Black dots are 10 nm gold particle linked to the exosome marker CD63. (C) Tunable resistive pulse sensing (TRPS) analysis showed a slightly reduction in EVs modal diameter in high intensity exercised group compared to non-exercised ( p = 0.057) and moderate intensity exercised groups ( p = 0.028). The median groups for EVs modal diameter range from 88 nm in non-exercised, through 87 nm in low intensity, 91.5 nm in moderate intensity and 85 nm in high intensity exercised group (D) . The concentration of EVs purified from rat’s serum after exercise measured by TRPS. There is a statistical increase in median EVs concentration after exercise, which range from 1.1 × 10 9 in non-exercised group to 3 × 10 9 in low intensity exercised group ( p = 0.014), 2.5 × 10 9 in moderate intensity exercised group ( p = 0.021) and 3 × 10 9 in high intensity exercised group ( p = 0.02). (E) The median concentration of rat serum EVs proteins. Proteins were purified from EVs and quantified. There is a statistical significance increase in EVs protein concentration median after exercise ranging from 0.935 mg.mL -1 in non-exercised group to 4.33 mg.mL -1 in low intensity exercised group ( p = 0.014), 4.31 mg.mL -1 in moderate intensity exercised group ( p = 0.014) and 4.31 mg.mL -1 in high intensity exercised group ( p = 0.014). (F) Total rat serum protein profile after Exoquick purification analyzed by SDS-PAGE 12%. Exoquick fraction contains EVs proteins while supernatant fraction contains free proteins that were not precipitated by Exoquick. (G) Western blotting of EVs proteins shows an increase in the exosome marker CD63 while increasing the intensity of exercise. ApoA-IV protein levels remained stable regardless of exercise intensity. Calnexin was used to show the absence of endoplasmic reticulum proteins in purified EVs. (H) The concentration of EVs small RNAs from rat’s serum. Small RNAs were purified, quantified and characterized by 2100 Bioanalyzer (Agilent) using a small RNA chip. There is no statistical difference between group medians.

Techniques Used: Concentration Assay, Purification, Marker, Tunable Resistive Pulse Sensing, Protein Concentration, SDS Page, Western Blot, Chromatin Immunoprecipitation

7) Product Images from "Effects of Acute Aerobic Exercise on Rats Serum Extracellular Vesicles Diameter, Concentration and Small RNAs Content"

Article Title: Effects of Acute Aerobic Exercise on Rats Serum Extracellular Vesicles Diameter, Concentration and Small RNAs Content

Journal: Frontiers in Physiology

doi: 10.3389/fphys.2018.00532

Acute aerobic exercise decreases rat serum EVs modal diameter and increases serum EVs concentration. (A) Workflow representing the acute exercise protocol used to provide aerobic exercise for Wistar rats running on a treadmill. (B) Immunoelectron micrograph of rat serum EVs purified by Exoquick. Black dots are 10 nm gold particle linked to the exosome marker CD63. (C) Tunable resistive pulse sensing (TRPS) analysis showed a slightly reduction in EVs modal diameter in high intensity exercised group compared to non-exercised ( p = 0.057) and moderate intensity exercised groups ( p = 0.028). The median groups for EVs modal diameter range from 88 nm in non-exercised, through 87 nm in low intensity, 91.5 nm in moderate intensity and 85 nm in high intensity exercised group (D) . The concentration of EVs purified from rat’s serum after exercise measured by TRPS. There is a statistical increase in median EVs concentration after exercise, which range from 1.1 × 10 9 in non-exercised group to 3 × 10 9 in low intensity exercised group ( p = 0.014), 2.5 × 10 9 in moderate intensity exercised group ( p = 0.021) and 3 × 10 9 in high intensity exercised group ( p = 0.02). (E) The median concentration of rat serum EVs proteins. Proteins were purified from EVs and quantified. There is a statistical significance increase in EVs protein concentration median after exercise ranging from 0.935 mg.mL -1 in non-exercised group to 4.33 mg.mL -1 in low intensity exercised group ( p = 0.014), 4.31 mg.mL -1 in moderate intensity exercised group ( p = 0.014) and 4.31 mg.mL -1 in high intensity exercised group ( p = 0.014). (F) Total rat serum protein profile after Exoquick purification analyzed by SDS-PAGE 12%. Exoquick fraction contains EVs proteins while supernatant fraction contains free proteins that were not precipitated by Exoquick. (G) Western blotting of EVs proteins shows an increase in the exosome marker CD63 while increasing the intensity of exercise. ApoA-IV protein levels remained stable regardless of exercise intensity. Calnexin was used to show the absence of endoplasmic reticulum proteins in purified EVs. (H) The concentration of EVs small RNAs from rat’s serum. Small RNAs were purified, quantified and characterized by 2100 Bioanalyzer (Agilent) using a small RNA chip. There is no statistical difference between group medians.
Figure Legend Snippet: Acute aerobic exercise decreases rat serum EVs modal diameter and increases serum EVs concentration. (A) Workflow representing the acute exercise protocol used to provide aerobic exercise for Wistar rats running on a treadmill. (B) Immunoelectron micrograph of rat serum EVs purified by Exoquick. Black dots are 10 nm gold particle linked to the exosome marker CD63. (C) Tunable resistive pulse sensing (TRPS) analysis showed a slightly reduction in EVs modal diameter in high intensity exercised group compared to non-exercised ( p = 0.057) and moderate intensity exercised groups ( p = 0.028). The median groups for EVs modal diameter range from 88 nm in non-exercised, through 87 nm in low intensity, 91.5 nm in moderate intensity and 85 nm in high intensity exercised group (D) . The concentration of EVs purified from rat’s serum after exercise measured by TRPS. There is a statistical increase in median EVs concentration after exercise, which range from 1.1 × 10 9 in non-exercised group to 3 × 10 9 in low intensity exercised group ( p = 0.014), 2.5 × 10 9 in moderate intensity exercised group ( p = 0.021) and 3 × 10 9 in high intensity exercised group ( p = 0.02). (E) The median concentration of rat serum EVs proteins. Proteins were purified from EVs and quantified. There is a statistical significance increase in EVs protein concentration median after exercise ranging from 0.935 mg.mL -1 in non-exercised group to 4.33 mg.mL -1 in low intensity exercised group ( p = 0.014), 4.31 mg.mL -1 in moderate intensity exercised group ( p = 0.014) and 4.31 mg.mL -1 in high intensity exercised group ( p = 0.014). (F) Total rat serum protein profile after Exoquick purification analyzed by SDS-PAGE 12%. Exoquick fraction contains EVs proteins while supernatant fraction contains free proteins that were not precipitated by Exoquick. (G) Western blotting of EVs proteins shows an increase in the exosome marker CD63 while increasing the intensity of exercise. ApoA-IV protein levels remained stable regardless of exercise intensity. Calnexin was used to show the absence of endoplasmic reticulum proteins in purified EVs. (H) The concentration of EVs small RNAs from rat’s serum. Small RNAs were purified, quantified and characterized by 2100 Bioanalyzer (Agilent) using a small RNA chip. There is no statistical difference between group medians.

Techniques Used: Concentration Assay, Purification, Marker, Tunable Resistive Pulse Sensing, Protein Concentration, SDS Page, Western Blot, Chromatin Immunoprecipitation

8) Product Images from "A highly effective and versatile technology for the isolation of RNAs from grapevines and other woody perennials for use in virus diagnostics"

Article Title: A highly effective and versatile technology for the isolation of RNAs from grapevines and other woody perennials for use in virus diagnostics

Journal: Virology Journal

doi: 10.1186/s12985-015-0376-3

Profile of total RNA isolated by using five commercial kits. a Denaturing gel electrophoresis of total RNA isolated from peach. b Denaturing gel electrophoresis of total RNA from grapevine leaves. 50 mg of young peach and grapevine leaves (indicated as Y), and mature (M) grapevine leaves was used in RNA isolation with Spectrum™ Plant Total RNA kit (Sigma), RNeasy Plant mini kit (Qiagen), Plant/fungi total RNA kit (Norgen), AccuPrep viral RNA extraction kit (Bioneer) and TRIzol Reagent (Life Technologies). The total RNA yield (μg), A260/A280 and A260/A230 ratios averaged from two replicates are given below each gel panel. 28S rRNA, 18S rRNA and small RNAs are indicated with arrows. c Capillary electrophoresis of total RNA with Agilent Bioanalyzer. One ml of each of the total RNA preparations isolated using these five systems was used for the analysis with an Agilent Bioanalyzer 2100 equipped with an RNA Nano chip
Figure Legend Snippet: Profile of total RNA isolated by using five commercial kits. a Denaturing gel electrophoresis of total RNA isolated from peach. b Denaturing gel electrophoresis of total RNA from grapevine leaves. 50 mg of young peach and grapevine leaves (indicated as Y), and mature (M) grapevine leaves was used in RNA isolation with Spectrum™ Plant Total RNA kit (Sigma), RNeasy Plant mini kit (Qiagen), Plant/fungi total RNA kit (Norgen), AccuPrep viral RNA extraction kit (Bioneer) and TRIzol Reagent (Life Technologies). The total RNA yield (μg), A260/A280 and A260/A230 ratios averaged from two replicates are given below each gel panel. 28S rRNA, 18S rRNA and small RNAs are indicated with arrows. c Capillary electrophoresis of total RNA with Agilent Bioanalyzer. One ml of each of the total RNA preparations isolated using these five systems was used for the analysis with an Agilent Bioanalyzer 2100 equipped with an RNA Nano chip

Techniques Used: Isolation, Nucleic Acid Electrophoresis, RNA Extraction, Electrophoresis, Chromatin Immunoprecipitation

9) Product Images from "Identification of deleterious rare variants in MTTP, PNPLA3, and TM6SF2 in Japanese males and association studies with NAFLD"

Article Title: Identification of deleterious rare variants in MTTP, PNPLA3, and TM6SF2 in Japanese males and association studies with NAFLD

Journal: Lipids in Health and Disease

doi: 10.1186/s12944-017-0570-y

Functional assessment of variants at intron-exon junctions. a Intron 6 to intron 8 of TM6SF2 was inserted into intron 1 of ACKR1 under control of the CMV promoter. b Intron 4 to 5 of PNPLA3 was inserted into intron 1 of TRIB2 . The constructs were transfected into Huh-7 cells. Twenty-four hours after transfection, total RNA was extracted from the transfected cells, and the corresponding cDNA was used as a template for RT-PCR. The amplicon sizes of each RT-PCR product were measured with a 2100 BioAnalyzer. Estimated splicing variants from amplicon sizes are shown on the left side
Figure Legend Snippet: Functional assessment of variants at intron-exon junctions. a Intron 6 to intron 8 of TM6SF2 was inserted into intron 1 of ACKR1 under control of the CMV promoter. b Intron 4 to 5 of PNPLA3 was inserted into intron 1 of TRIB2 . The constructs were transfected into Huh-7 cells. Twenty-four hours after transfection, total RNA was extracted from the transfected cells, and the corresponding cDNA was used as a template for RT-PCR. The amplicon sizes of each RT-PCR product were measured with a 2100 BioAnalyzer. Estimated splicing variants from amplicon sizes are shown on the left side

Techniques Used: Functional Assay, Construct, Transfection, Reverse Transcription Polymerase Chain Reaction, Amplification

10) Product Images from "Effect of lesion proximity on the regenerative response of long descending propriospinal neurons after spinal transection injury"

Article Title: Effect of lesion proximity on the regenerative response of long descending propriospinal neurons after spinal transection injury

Journal: BMC Neuroscience

doi: 10.1186/s12868-019-0491-y

RNA Quality Pseudogel and R.I.N. Fluorogold retrograde labelled neurons were collected by laser capture microdissection, and processed to collect the RNA that was used to measure the changes in genetic expression. The quality of the RNA was assessed using the Qiagen 2100 bioanalyzer (Agilent Technologies; Santa Clara, CA) which provided both an RNA Integrity Number (RIN), and corresponding pseudo gel. L = Ladder, C = Control Animal, and I = Animal receiving spinal transection injury
Figure Legend Snippet: RNA Quality Pseudogel and R.I.N. Fluorogold retrograde labelled neurons were collected by laser capture microdissection, and processed to collect the RNA that was used to measure the changes in genetic expression. The quality of the RNA was assessed using the Qiagen 2100 bioanalyzer (Agilent Technologies; Santa Clara, CA) which provided both an RNA Integrity Number (RIN), and corresponding pseudo gel. L = Ladder, C = Control Animal, and I = Animal receiving spinal transection injury

Techniques Used: Laser Capture Microdissection, Expressing

11) Product Images from "VR09 Cell Line: An EBV-Positive Lymphoblastoid Cell Line with In Vivo Characteristics of Diffuse Large B Cell Lymphoma of Activated B-Cell Type"

Article Title: VR09 Cell Line: An EBV-Positive Lymphoblastoid Cell Line with In Vivo Characteristics of Diffuse Large B Cell Lymphoma of Activated B-Cell Type

Journal: PLoS ONE

doi: 10.1371/journal.pone.0052811

EBV positivity in the original tumor and in the VR09 cell line. PCR products analysis by Agilent 2100 Bioanalyzer showed the presence of the same 151 bp specific amplicon for EBV RPMS1 gene, thus demonstrating that EBV infection was present in the original cells from patient. A normal DNA from pancreas was used as negative control.
Figure Legend Snippet: EBV positivity in the original tumor and in the VR09 cell line. PCR products analysis by Agilent 2100 Bioanalyzer showed the presence of the same 151 bp specific amplicon for EBV RPMS1 gene, thus demonstrating that EBV infection was present in the original cells from patient. A normal DNA from pancreas was used as negative control.

Techniques Used: Polymerase Chain Reaction, Amplification, Infection, Negative Control

12) Product Images from "A streamlined method for analysing genome-wide DNA methylation patterns from low amounts of FFPE DNA"

Article Title: A streamlined method for analysing genome-wide DNA methylation patterns from low amounts of FFPE DNA

Journal: BMC Medical Genomics

doi: 10.1186/s12920-017-0290-1

Bioanalyser images demonstrating quality of two FFPE RRBS libraries. Each of the RRBS libraries (FFPE1 and FFPE2) was run on an Agilent 2100 Bioanalyzer using the high sensitivity DNA kit. The electropherogram displays a plot of fragment size (bp) versus fluorescence intensity. Peaks at 35 bp and 10,380 bp represent lower and upper markers. The 160–340 bp peaks represent the RRBS library
Figure Legend Snippet: Bioanalyser images demonstrating quality of two FFPE RRBS libraries. Each of the RRBS libraries (FFPE1 and FFPE2) was run on an Agilent 2100 Bioanalyzer using the high sensitivity DNA kit. The electropherogram displays a plot of fragment size (bp) versus fluorescence intensity. Peaks at 35 bp and 10,380 bp represent lower and upper markers. The 160–340 bp peaks represent the RRBS library

Techniques Used: Formalin-fixed Paraffin-Embedded, Fluorescence

13) Product Images from "RNase L Interacts with Filamin A To Regulate Actin Dynamics and Barrier Function for Viral Entry"

Article Title: RNase L Interacts with Filamin A To Regulate Actin Dynamics and Barrier Function for Viral Entry

Journal: mBio

doi: 10.1128/mBio.02012-14

Effect of Filamin A and actin cytoskeleton on RNase L activity. M2 stable cell lines expressing Myc-Filamin A and/or Flag-RNase L WT or Flag-RNase L R667A were analyzed for expression of RNase L and Filamin A on immunoblots (A) and transfected with 10 µM 2-5A (B), and RNase L-mediated cleavage of rRNA (arrows) was analyzed on RNA chips using an Agilent 2100 Bioanalyzer. (C) HT1080 cells were pretreated with Cytochalasin D (Cyto D; 5 µM), Latrunculin A (Lat A; 0.5 µM), or vehicle (DMSO) for 1 h followed by transfection with 10 µM 2-5A or 2 µg/ml of poly(I·C). Characteristic RNase L-generated rRNA cleavage products (arrows) were detected as described for panel B. Representative images from three independent experiments are shown.
Figure Legend Snippet: Effect of Filamin A and actin cytoskeleton on RNase L activity. M2 stable cell lines expressing Myc-Filamin A and/or Flag-RNase L WT or Flag-RNase L R667A were analyzed for expression of RNase L and Filamin A on immunoblots (A) and transfected with 10 µM 2-5A (B), and RNase L-mediated cleavage of rRNA (arrows) was analyzed on RNA chips using an Agilent 2100 Bioanalyzer. (C) HT1080 cells were pretreated with Cytochalasin D (Cyto D; 5 µM), Latrunculin A (Lat A; 0.5 µM), or vehicle (DMSO) for 1 h followed by transfection with 10 µM 2-5A or 2 µg/ml of poly(I·C). Characteristic RNase L-generated rRNA cleavage products (arrows) were detected as described for panel B. Representative images from three independent experiments are shown.

Techniques Used: Activity Assay, Stable Transfection, Expressing, Western Blot, Transfection, Generated

14) Product Images from "Tropomyosin isoforms differentially affect muscle contractility in the head and body regions of the nematode Caenorhabditis elegans"

Article Title: Tropomyosin isoforms differentially affect muscle contractility in the head and body regions of the nematode Caenorhabditis elegans

Journal: Molecular Biology of the Cell

doi: 10.1091/mbc.E17-03-0152

Characterization of mutually exclusive seventh exons of the C. elegans lev-11 tropomyosin gene. (A) Structure of the lev-11 gene is shown schematically (top) with numbered boxes indicating exons. Recently characterized alternative exons 7a and 7b are shown in green. Below the gene structure are splicing patterns of LEV-11A/CeTMI, a newly identified isoform, LEV-11O, and three previously characterized isoforms, LEV-11D/CeTMII, LEV-11E/CeTMIII, and LEV-11C/CeTMIV. Coding and noncoding regions are shown in orange and light yellow, respectively. Note that exon 9c is used in all known isoforms either as a coding region (LEV-11A and LEV-11O) or as a noncoding region when 9a or 9b is used as a coding region (LEV-11C, LEV-11D, and LEV-11E). (B) Analysis of lev-11 mRNAs by RT-PCR. Total RNAs from synchronized wild-type L1 larvae were subjected to RT-PCR with indicated primer pairs and cycle numbers, and the PCR products were analyzed with a 2100 BioAnalyzer (Agilent). DNA size markers are shown on the left. Results are shown in gel-like presentations. Exon combinations of representative bands a–d are shown below. Multiple bands in E7b/E9c (lane 6) were cloned and sequenced, and the exon combinations are indicated on the right. Asterisks indicate artificial PCR products due to excessive cycles. (C) Alignment of amino acid sequences encoded by exons 7a and 7b. Identical residues are indicated with black backgrounds. Point mutations in lev-11(gk334531) and lev-11(x12) are shown at the top and bottom of the sequences, respectively. (D) Probability of coiled-coil formation (0–1) was calculated from the full-length sequences of LEV-11A and LEV-11O by COILS ( Lupas et al. , 1991 ), and plots of exon 7-coded regions are shown.
Figure Legend Snippet: Characterization of mutually exclusive seventh exons of the C. elegans lev-11 tropomyosin gene. (A) Structure of the lev-11 gene is shown schematically (top) with numbered boxes indicating exons. Recently characterized alternative exons 7a and 7b are shown in green. Below the gene structure are splicing patterns of LEV-11A/CeTMI, a newly identified isoform, LEV-11O, and three previously characterized isoforms, LEV-11D/CeTMII, LEV-11E/CeTMIII, and LEV-11C/CeTMIV. Coding and noncoding regions are shown in orange and light yellow, respectively. Note that exon 9c is used in all known isoforms either as a coding region (LEV-11A and LEV-11O) or as a noncoding region when 9a or 9b is used as a coding region (LEV-11C, LEV-11D, and LEV-11E). (B) Analysis of lev-11 mRNAs by RT-PCR. Total RNAs from synchronized wild-type L1 larvae were subjected to RT-PCR with indicated primer pairs and cycle numbers, and the PCR products were analyzed with a 2100 BioAnalyzer (Agilent). DNA size markers are shown on the left. Results are shown in gel-like presentations. Exon combinations of representative bands a–d are shown below. Multiple bands in E7b/E9c (lane 6) were cloned and sequenced, and the exon combinations are indicated on the right. Asterisks indicate artificial PCR products due to excessive cycles. (C) Alignment of amino acid sequences encoded by exons 7a and 7b. Identical residues are indicated with black backgrounds. Point mutations in lev-11(gk334531) and lev-11(x12) are shown at the top and bottom of the sequences, respectively. (D) Probability of coiled-coil formation (0–1) was calculated from the full-length sequences of LEV-11A and LEV-11O by COILS ( Lupas et al. , 1991 ), and plots of exon 7-coded regions are shown.

Techniques Used: Reverse Transcription Polymerase Chain Reaction, Polymerase Chain Reaction, Clone Assay

15) Product Images from "Dynamic Reorganization of Nucleosome Positioning in Somatic Cells after Transfer into Porcine Enucleated Oocytes"

Article Title: Dynamic Reorganization of Nucleosome Positioning in Somatic Cells after Transfer into Porcine Enucleated Oocytes

Journal: Stem Cell Reports

doi: 10.1016/j.stemcr.2017.06.004

Establishment of MNase-Seq Using 1,000 Cells (A) Isolation of mononucleosomes by MNase digestion of 10 6 PEF. M, 100-bp ladder; 1–2, 10 6 PEF; 1N, isolation of mononucleosomes; 2N, isolation of dinucleosomes. (B) Detection of the adaptor-ligated mononucleosome library derived from 1,000 PEF with the Agilent 2100 Bioanalyzer. (C) Heatmap of the Pearson correlations among PEF-1, PEF-2, and PEF-3.
Figure Legend Snippet: Establishment of MNase-Seq Using 1,000 Cells (A) Isolation of mononucleosomes by MNase digestion of 10 6 PEF. M, 100-bp ladder; 1–2, 10 6 PEF; 1N, isolation of mononucleosomes; 2N, isolation of dinucleosomes. (B) Detection of the adaptor-ligated mononucleosome library derived from 1,000 PEF with the Agilent 2100 Bioanalyzer. (C) Heatmap of the Pearson correlations among PEF-1, PEF-2, and PEF-3.

Techniques Used: Isolation, Derivative Assay

16) Product Images from "Compound C Prevents the Unfolded Protein Response during Glucose Deprivation through a Mechanism Independent of AMPK and BMP Signaling"

Article Title: Compound C Prevents the Unfolded Protein Response during Glucose Deprivation through a Mechanism Independent of AMPK and BMP Signaling

Journal: PLoS ONE

doi: 10.1371/journal.pone.0045845

Effects of compound C on the UPR signaling pathway. ( A – C ) Immunoblot analysis of UPR-related proteins. In A, HT1080 ( right ) and 786-O cells ( left ) were treated for 18 h with compound C, versipelostatin or phenformin in the presence (+) or absence (−) of 10 mM 2DG. In B, HT1080 cells were transfected with 100 ng of 7× FLAG-tagged ATF6 plasmid (expressed FLAG-tagged full-length p90ATF6) and treated for 6 h with compound C or versipelostatin in the presence (+) or absence (−) of 10 mM 2DG. For better detection of the p50ATF6/active form, MG132 was included during exposure of cells to 2DG. In C, 786-O cells were treated for 6 h with compound C and versipelostatin in the presence (+) or absence (−) of 10 mM 2DG and MG132. β-actin was used as a loading control. (D) XBP1 reporter assay. XBP1-Luc/HT1080 cells were transfected with phRL-CMV and exposed to stress (10 mM 2DG or 5 µg/mL of tunicamycin) for 18 h with compound C and versipelostatin. Results shown are the means ± SD of quadruplicate determinations. (E, F) Quantitative PCR (E) and real-time PCR (F) analysis of XBP1 transcript. HT1080 cells were treated with compound C and versipelostatin for 18 h under normal or 2DG stress conditions. In E , PCR products were analyzed by Agilent 2100 Bioanalyzer (gel-like image, upper ). Relative ratios of XBP1 mRNA splicing valiant were calculated by setting each total expression amount of XBP1 mRNA in cells as 1 (graph, lower ). Two independent experiments were performed to confirm the reproducibility. In F, relative expression levels of endogenous XBP1 mRNA were calculated by setting each normal expression level from non–drug-treated cells as 1. Results shown are the means ± SD. (G) Immunoblot analysis of UPR-related proteins. TIG-3 cells were treated for 18 h with compound C, versipelostatin or phenformin in the presence (+) or absence (−) of 10 mM 2DG. β-actin was used as a loading control. 2DG, 2-deoxy-D-glucose; TM, tunicamycin; CC, compound C; VST, versipelostatin; Phen, phenformin.
Figure Legend Snippet: Effects of compound C on the UPR signaling pathway. ( A – C ) Immunoblot analysis of UPR-related proteins. In A, HT1080 ( right ) and 786-O cells ( left ) were treated for 18 h with compound C, versipelostatin or phenformin in the presence (+) or absence (−) of 10 mM 2DG. In B, HT1080 cells were transfected with 100 ng of 7× FLAG-tagged ATF6 plasmid (expressed FLAG-tagged full-length p90ATF6) and treated for 6 h with compound C or versipelostatin in the presence (+) or absence (−) of 10 mM 2DG. For better detection of the p50ATF6/active form, MG132 was included during exposure of cells to 2DG. In C, 786-O cells were treated for 6 h with compound C and versipelostatin in the presence (+) or absence (−) of 10 mM 2DG and MG132. β-actin was used as a loading control. (D) XBP1 reporter assay. XBP1-Luc/HT1080 cells were transfected with phRL-CMV and exposed to stress (10 mM 2DG or 5 µg/mL of tunicamycin) for 18 h with compound C and versipelostatin. Results shown are the means ± SD of quadruplicate determinations. (E, F) Quantitative PCR (E) and real-time PCR (F) analysis of XBP1 transcript. HT1080 cells were treated with compound C and versipelostatin for 18 h under normal or 2DG stress conditions. In E , PCR products were analyzed by Agilent 2100 Bioanalyzer (gel-like image, upper ). Relative ratios of XBP1 mRNA splicing valiant were calculated by setting each total expression amount of XBP1 mRNA in cells as 1 (graph, lower ). Two independent experiments were performed to confirm the reproducibility. In F, relative expression levels of endogenous XBP1 mRNA were calculated by setting each normal expression level from non–drug-treated cells as 1. Results shown are the means ± SD. (G) Immunoblot analysis of UPR-related proteins. TIG-3 cells were treated for 18 h with compound C, versipelostatin or phenformin in the presence (+) or absence (−) of 10 mM 2DG. β-actin was used as a loading control. 2DG, 2-deoxy-D-glucose; TM, tunicamycin; CC, compound C; VST, versipelostatin; Phen, phenformin.

Techniques Used: Transfection, Plasmid Preparation, Reporter Assay, Real-time Polymerase Chain Reaction, Polymerase Chain Reaction, Expressing

17) Product Images from "Different gDNA Content in the Subpopulations of Prostate Cancer Extracellular Vesicles: Apoptotic Bodies, Microvesicles, and Exosomes"

Article Title: Different gDNA Content in the Subpopulations of Prostate Cancer Extracellular Vesicles: Apoptotic Bodies, Microvesicles, and Exosomes

Journal: The Prostate

doi: 10.1002/pros.22853

Bioanalyzer analysis of gDNA in plasma-derived EVs from prostate cancer patients. Pre-amplified EV-derived DNA isolated from 2 mL of plasma together with DNA markers were analyzed using a 2100 Bioanalyzer. Vertical axis (FU) represents the fluorescent units and horizontal axis shows the number of base pairs. The two picks at 50 and 10,380 bp represent the DNA markers. Apoptotic bodies (ABs); microvesicles (MVs); exosomes (EXOs). Representative images of prostate cancer patients are showed.
Figure Legend Snippet: Bioanalyzer analysis of gDNA in plasma-derived EVs from prostate cancer patients. Pre-amplified EV-derived DNA isolated from 2 mL of plasma together with DNA markers were analyzed using a 2100 Bioanalyzer. Vertical axis (FU) represents the fluorescent units and horizontal axis shows the number of base pairs. The two picks at 50 and 10,380 bp represent the DNA markers. Apoptotic bodies (ABs); microvesicles (MVs); exosomes (EXOs). Representative images of prostate cancer patients are showed.

Techniques Used: Derivative Assay, Amplification, Isolation

18) Product Images from "High-throughput sequencing of human plasma RNA by using thermostable group II intron reverse transcriptases"

Article Title: High-throughput sequencing of human plasma RNA by using thermostable group II intron reverse transcriptases

Journal: RNA

doi: 10.1261/rna.054809.115

Bioanalyzer traces showing size profiles of plasma RNAs before and after various treatments. Total plasma RNA was prepared by the Direct-zol method, and a 1-µL portion was analyzed with an RNA 6000 Pico Kit (mRNA assay) on a 2100 Bioanalyzer (Agilent)
Figure Legend Snippet: Bioanalyzer traces showing size profiles of plasma RNAs before and after various treatments. Total plasma RNA was prepared by the Direct-zol method, and a 1-µL portion was analyzed with an RNA 6000 Pico Kit (mRNA assay) on a 2100 Bioanalyzer (Agilent)

Techniques Used:

19) Product Images from "Transcriptomic analyses of Hand2 transgenic embryos"

Article Title: Transcriptomic analyses of Hand2 transgenic embryos

Journal: Genomics Data

doi: 10.1016/j.gdata.2016.06.015

RNA quality control. A. RNA quality was measured using the Agilent 2100 Bioanalyzer for wild-type (a, c) and Hand2 NC mutant (b, d) samples at E11.5 (a, b) and E12.5 (c, d). The RNA Integrity Number (RIN; value assigned from 0 to 10) and histograms are shown. B. Scatter plots showing the correlation of signal values between two samples from E11.5 (a) and E12.5 (b) embryos. Data assigned to absent calls were omitted. C. qPCR analysis of the Hand2 transcript levels from wild-type (WT) and Hand2 NC (Tg) embryos at E11.5 (a) and E12.5 (b). Hand2 expression was upregulated in the Hand2 NC embryos. The experimental data were analyzed using two-tailed Student's t -tests and were expressed as the mean ± standard error of the mean (SEM). P -values less than 0.05 were considered as significant.
Figure Legend Snippet: RNA quality control. A. RNA quality was measured using the Agilent 2100 Bioanalyzer for wild-type (a, c) and Hand2 NC mutant (b, d) samples at E11.5 (a, b) and E12.5 (c, d). The RNA Integrity Number (RIN; value assigned from 0 to 10) and histograms are shown. B. Scatter plots showing the correlation of signal values between two samples from E11.5 (a) and E12.5 (b) embryos. Data assigned to absent calls were omitted. C. qPCR analysis of the Hand2 transcript levels from wild-type (WT) and Hand2 NC (Tg) embryos at E11.5 (a) and E12.5 (b). Hand2 expression was upregulated in the Hand2 NC embryos. The experimental data were analyzed using two-tailed Student's t -tests and were expressed as the mean ± standard error of the mean (SEM). P -values less than 0.05 were considered as significant.

Techniques Used: Mutagenesis, Real-time Polymerase Chain Reaction, Expressing, Two Tailed Test

20) Product Images from "Gene expression profiling of the olfactory tissues of sex-separated and sex-combined female and male mice"

Article Title: Gene expression profiling of the olfactory tissues of sex-separated and sex-combined female and male mice

Journal: Scientific Data

doi: 10.1038/sdata.2018.260

RNA integrity analysis. The integrity of ( a ) MOE, ( b ) VNO, and ( c ) OB samples was analyzed using an Agilent Bioanalyzer 2100 instrument. RNA integrity number (RIN) values for all samples are listed in Table 1 .
Figure Legend Snippet: RNA integrity analysis. The integrity of ( a ) MOE, ( b ) VNO, and ( c ) OB samples was analyzed using an Agilent Bioanalyzer 2100 instrument. RNA integrity number (RIN) values for all samples are listed in Table 1 .

Techniques Used:

21) Product Images from "The stage-specific testicular germ cell apoptotic response to low dose X-irradiation and 2,5-hexanedione combined exposure. I. Validation of the laser capture microdissection method for qRT-PCR array application"

Article Title: The stage-specific testicular germ cell apoptotic response to low dose X-irradiation and 2,5-hexanedione combined exposure. I. Validation of the laser capture microdissection method for qRT-PCR array application

Journal: Toxicologic pathology

doi: 10.1177/0192623314526319

LCM-derived seminiferous tubule RNA quality assessment. Digital gel (A) and electropherogram results (B–C) obtained with the Agilent 2100 Bioanalyzer. Electropherogram results are shown for before (B) and after (C) DNase treatment and RNA concentration,
Figure Legend Snippet: LCM-derived seminiferous tubule RNA quality assessment. Digital gel (A) and electropherogram results (B–C) obtained with the Agilent 2100 Bioanalyzer. Electropherogram results are shown for before (B) and after (C) DNase treatment and RNA concentration,

Techniques Used: Laser Capture Microdissection, Derivative Assay, Concentration Assay

22) Product Images from "Iterative Fragmentation Improves the Detection of ChIP-seq Peaks for Inactive Histone Marks"

Article Title: Iterative Fragmentation Improves the Detection of ChIP-seq Peaks for Inactive Histone Marks

Journal: Bioinformatics and Biology Insights

doi: 10.4137/BBI.S40628

Overview and effect of the reshearing method. ( A ) The relevant steps of ChIP-seq sample preparation, using the traditional method. After the fragmentation of the protein, we have a mixture of fragments of different sizes. The ones that carry our protein of interest can be bound by immunoprecipitation. After the decrosslinking and purification step, we get the DNA fragments, where the ones over the optimal size range are shown in red, and the ones under the size range are in green for better visual interpretation. During the size selection before library preparation and sequencing, these fragments are discarded; thus, a significant amount of the sample is lost. ( B ) The reshearing method preserves the fragments that are out of the ideal size range. By doing additional rounds of sonication on the eluted DNA, the long fragments break up into shorter ones (see the fragments in red), which enables them to proceed to library preparation and sonication. Sample loss is reduced significantly. ( C – E ) Demonstrating the effect of the reshearing on the actual H3K27me3 sample. The fragment range distribution is measured by a 2100 Bioanalyzer, images were generated by its software provided by Agilent; the control marks are at 35 bp and 10380 bp. ( C ) The original fragment distribution, before the reshearing step. ( D ) The size distribution after two rounds of five cycles of reshearing. A reduction of the large peak in the large size range and a slight shift toward the smaller sizes is already visible. ( E ) The distribution after the third round of reshearing. Here the shift is already complete: the large fragments have disappeared and the middle short section of the size range is enlarged, showing that we have reached the desired size distribution.
Figure Legend Snippet: Overview and effect of the reshearing method. ( A ) The relevant steps of ChIP-seq sample preparation, using the traditional method. After the fragmentation of the protein, we have a mixture of fragments of different sizes. The ones that carry our protein of interest can be bound by immunoprecipitation. After the decrosslinking and purification step, we get the DNA fragments, where the ones over the optimal size range are shown in red, and the ones under the size range are in green for better visual interpretation. During the size selection before library preparation and sequencing, these fragments are discarded; thus, a significant amount of the sample is lost. ( B ) The reshearing method preserves the fragments that are out of the ideal size range. By doing additional rounds of sonication on the eluted DNA, the long fragments break up into shorter ones (see the fragments in red), which enables them to proceed to library preparation and sonication. Sample loss is reduced significantly. ( C – E ) Demonstrating the effect of the reshearing on the actual H3K27me3 sample. The fragment range distribution is measured by a 2100 Bioanalyzer, images were generated by its software provided by Agilent; the control marks are at 35 bp and 10380 bp. ( C ) The original fragment distribution, before the reshearing step. ( D ) The size distribution after two rounds of five cycles of reshearing. A reduction of the large peak in the large size range and a slight shift toward the smaller sizes is already visible. ( E ) The distribution after the third round of reshearing. Here the shift is already complete: the large fragments have disappeared and the middle short section of the size range is enlarged, showing that we have reached the desired size distribution.

Techniques Used: Chromatin Immunoprecipitation, Sample Prep, Immunoprecipitation, Purification, Selection, Sequencing, Sonication, Generated, Software

23) Product Images from "Defining nonsense-mediated mRNA decay intermediates in human cells"

Article Title: Defining nonsense-mediated mRNA decay intermediates in human cells

Journal: Methods (San Diego, Calif.)

doi: 10.1016/j.ymeth.2018.12.005

Quality check of IP specificity and first and second PCR-sample sizes and amounts used for NMD-DegSeq. (A) Western blot of lysates of HEK293T cells using the specified antibodies prior to (Input) or after IP using anti(α)-p-UFP1 S1116 antibody (i.e. (α)-p-UFPl) or, as a negative control, rabbit (r)IgG. The left-most five lanes are 3-fold dilutions of Input sample (i.e. before IP). (B) SYBR Gold staining after the second PCR-amplification for the purpose of quality control. Analyses are of RNA samples prior to (Input); after (+) IP using anti(α)-p-UFP1 S1116 antibody or, as a negative control, rabbit (r)IgG; or no RNA (None). These RNAs were subjected to the first PCR and, subsequently, as second PCR, after which PCR products were (+) or were not (−) size-selected (the latter has Adapter self-ligation products that should be eliminated prior to library construction). (C) Agilent Bioanalyzer 2100 analyses using capillary electrophoresis (upper) and tracing (lower) of fluorescent samples after the first PCR. Results confirm adequate quality and quantity of samples before (Input) and after IP so that index PCR can be performed to generate samples for Illumina sequencing. FU, fluorescence units; bp, base pairs.
Figure Legend Snippet: Quality check of IP specificity and first and second PCR-sample sizes and amounts used for NMD-DegSeq. (A) Western blot of lysates of HEK293T cells using the specified antibodies prior to (Input) or after IP using anti(α)-p-UFP1 S1116 antibody (i.e. (α)-p-UFPl) or, as a negative control, rabbit (r)IgG. The left-most five lanes are 3-fold dilutions of Input sample (i.e. before IP). (B) SYBR Gold staining after the second PCR-amplification for the purpose of quality control. Analyses are of RNA samples prior to (Input); after (+) IP using anti(α)-p-UFP1 S1116 antibody or, as a negative control, rabbit (r)IgG; or no RNA (None). These RNAs were subjected to the first PCR and, subsequently, as second PCR, after which PCR products were (+) or were not (−) size-selected (the latter has Adapter self-ligation products that should be eliminated prior to library construction). (C) Agilent Bioanalyzer 2100 analyses using capillary electrophoresis (upper) and tracing (lower) of fluorescent samples after the first PCR. Results confirm adequate quality and quantity of samples before (Input) and after IP so that index PCR can be performed to generate samples for Illumina sequencing. FU, fluorescence units; bp, base pairs.

Techniques Used: Polymerase Chain Reaction, Western Blot, Negative Control, Staining, Amplification, Ligation, Electrophoresis, Sequencing, Fluorescence

24) Product Images from "Investigation of Content, Stoichiometry and Transfer of miRNA from Human Neural Stem Cell Line Derived Exosomes"

Article Title: Investigation of Content, Stoichiometry and Transfer of miRNA from Human Neural Stem Cell Line Derived Exosomes

Journal: PLoS ONE

doi: 10.1371/journal.pone.0146353

MiRNA next generation sequencing. Cellular (A) and exosomal (B) total RNAs were processed by an Agilent 2100 Bioanalyzer. The corresponding virtual gel images generated by the software are depicted as electropherograms. (C) Representative diagram of differential miRNA distribution in exosomes compared to hNSC producers. MiRNA types preferentially released in exosomes are presented in red or retained within the hNSCs in blue, data expressed as log 2 ratio of exosomal/cellular miRNAs normalized read counts. Pie chart representation of the distribution of small RNA categories in hNSC (D) and exosome (E) samples.
Figure Legend Snippet: MiRNA next generation sequencing. Cellular (A) and exosomal (B) total RNAs were processed by an Agilent 2100 Bioanalyzer. The corresponding virtual gel images generated by the software are depicted as electropherograms. (C) Representative diagram of differential miRNA distribution in exosomes compared to hNSC producers. MiRNA types preferentially released in exosomes are presented in red or retained within the hNSCs in blue, data expressed as log 2 ratio of exosomal/cellular miRNAs normalized read counts. Pie chart representation of the distribution of small RNA categories in hNSC (D) and exosome (E) samples.

Techniques Used: Next-Generation Sequencing, Generated, Software

25) Product Images from "Alternative splicing of the Caenorhabditis elegans lev-11 tropomyosin gene is regulated in a tissue-specific manner"

Article Title: Alternative splicing of the Caenorhabditis elegans lev-11 tropomyosin gene is regulated in a tissue-specific manner

Journal: Cytoskeleton (Hoboken, N.J.)

doi: 10.1002/cm.21489

Analysis of lev-11 mRNAs by RT-PCR. (Lanes 1–6) Total RNAs from synchronized wild-type L1 larvae were subjected to RT-PCR with indicated primer pairs (shown on top) and cycle numbers (shown on bottom), and the PCR products were analyzed with an on-chip capillary electrophoresis system, 2100 BioAnalyzer (Agilent). Results are shown in gel-like presentations. (Lanes 7–9) Total RNAs from mixed-stage wild-type worms were subjected to RT-PCR with indicated primer pairs (shown on top) and cycle numbers (shown on bottom), and the PCR products were analyzed with conventional agarose gel electrophoresis. Major cDNA species were cloned and sequenced, and identified isoforms are indicated. DNA size markers are shown on the left of lanes 1 and 7. The presented data are representative results from at least three separate experiments.
Figure Legend Snippet: Analysis of lev-11 mRNAs by RT-PCR. (Lanes 1–6) Total RNAs from synchronized wild-type L1 larvae were subjected to RT-PCR with indicated primer pairs (shown on top) and cycle numbers (shown on bottom), and the PCR products were analyzed with an on-chip capillary electrophoresis system, 2100 BioAnalyzer (Agilent). Results are shown in gel-like presentations. (Lanes 7–9) Total RNAs from mixed-stage wild-type worms were subjected to RT-PCR with indicated primer pairs (shown on top) and cycle numbers (shown on bottom), and the PCR products were analyzed with conventional agarose gel electrophoresis. Major cDNA species were cloned and sequenced, and identified isoforms are indicated. DNA size markers are shown on the left of lanes 1 and 7. The presented data are representative results from at least three separate experiments.

Techniques Used: Reverse Transcription Polymerase Chain Reaction, Polymerase Chain Reaction, Chromatin Immunoprecipitation, Electrophoresis, Agarose Gel Electrophoresis, Clone Assay

26) Product Images from "Thioredoxin A Active-Site Mutants Form Mixed Disulfide Dimers That Resemble Enzyme-Substrate Reaction Intermediates"

Article Title: Thioredoxin A Active-Site Mutants Form Mixed Disulfide Dimers That Resemble Enzyme-Substrate Reaction Intermediates

Journal: Journal of Molecular Biology

doi: 10.1016/j.jmb.2008.03.077

Redox states of BsTrxA monomers and dimers. (a) Purified His6-tagged BsTrxA proteins were separated by capillary electrophoresis using a 2100 Bioanalyzer (Agilent Technologies). WT, BsTrxA with wild-type active site; C29S, C29S single-mutant BsTrxA; C32S, C32S single-mutant BsTrxA; C29S–C32S, C29S–C32S double-mutant BsTrxA. To monitor the presence of free thiols in the purified BsTrxA proteins, samples were incubated in the presence or in the absence of 0.3 mM AMS (lanes marked + AMS or − AMS). To test whether the C29S and C32S dimers are formed by disulfide bonding, these proteins were incubated with 10 mM DTT (lanes marked + DTT). DTT was absent from all other samples. The image of the Bioanalyzer chromatogram was generated using the 2100 Expert Software package (Agilent Technologies). (b) C32S BsTrxA protein (∼ 2.5 μg) was reduced with increasing concentrations of DTT (shown on top of the panel) and separated by capillary electrophoresis as described for (a). The dimer-to-monomer ratios (Dimer [%]) are shown at the bottom of the panel.
Figure Legend Snippet: Redox states of BsTrxA monomers and dimers. (a) Purified His6-tagged BsTrxA proteins were separated by capillary electrophoresis using a 2100 Bioanalyzer (Agilent Technologies). WT, BsTrxA with wild-type active site; C29S, C29S single-mutant BsTrxA; C32S, C32S single-mutant BsTrxA; C29S–C32S, C29S–C32S double-mutant BsTrxA. To monitor the presence of free thiols in the purified BsTrxA proteins, samples were incubated in the presence or in the absence of 0.3 mM AMS (lanes marked + AMS or − AMS). To test whether the C29S and C32S dimers are formed by disulfide bonding, these proteins were incubated with 10 mM DTT (lanes marked + DTT). DTT was absent from all other samples. The image of the Bioanalyzer chromatogram was generated using the 2100 Expert Software package (Agilent Technologies). (b) C32S BsTrxA protein (∼ 2.5 μg) was reduced with increasing concentrations of DTT (shown on top of the panel) and separated by capillary electrophoresis as described for (a). The dimer-to-monomer ratios (Dimer [%]) are shown at the bottom of the panel.

Techniques Used: Purification, Electrophoresis, Mutagenesis, Incubation, Affinity Magnetic Separation, Generated, Software

27) Product Images from "Thioredoxin A Active-Site Mutants Form Mixed Disulfide Dimers That Resemble Enzyme-Substrate Reaction Intermediates"

Article Title: Thioredoxin A Active-Site Mutants Form Mixed Disulfide Dimers That Resemble Enzyme-Substrate Reaction Intermediates

Journal: Journal of Molecular Biology

doi: 10.1016/j.jmb.2008.03.077

Redox states of BsTrxA monomers and dimers. (a) Purified His6-tagged BsTrxA proteins were separated by capillary electrophoresis using a 2100 Bioanalyzer (Agilent Technologies). WT, BsTrxA with wild-type active site; C29S, C29S single-mutant BsTrxA; C32S, C32S single-mutant BsTrxA; C29S–C32S, C29S–C32S double-mutant BsTrxA. To monitor the presence of free thiols in the purified BsTrxA proteins, samples were incubated in the presence or in the absence of 0.3 mM AMS (lanes marked + AMS or − AMS). To test whether the C29S and C32S dimers are formed by disulfide bonding, these proteins were incubated with 10 mM DTT (lanes marked + DTT). DTT was absent from all other samples. The image of the Bioanalyzer chromatogram was generated using the 2100 Expert Software package (Agilent Technologies). (b) C32S BsTrxA protein (∼ 2.5 μg) was reduced with increasing concentrations of DTT (shown on top of the panel) and separated by capillary electrophoresis as described for (a). The dimer-to-monomer ratios (Dimer [%]) are shown at the bottom of the panel.
Figure Legend Snippet: Redox states of BsTrxA monomers and dimers. (a) Purified His6-tagged BsTrxA proteins were separated by capillary electrophoresis using a 2100 Bioanalyzer (Agilent Technologies). WT, BsTrxA with wild-type active site; C29S, C29S single-mutant BsTrxA; C32S, C32S single-mutant BsTrxA; C29S–C32S, C29S–C32S double-mutant BsTrxA. To monitor the presence of free thiols in the purified BsTrxA proteins, samples were incubated in the presence or in the absence of 0.3 mM AMS (lanes marked + AMS or − AMS). To test whether the C29S and C32S dimers are formed by disulfide bonding, these proteins were incubated with 10 mM DTT (lanes marked + DTT). DTT was absent from all other samples. The image of the Bioanalyzer chromatogram was generated using the 2100 Expert Software package (Agilent Technologies). (b) C32S BsTrxA protein (∼ 2.5 μg) was reduced with increasing concentrations of DTT (shown on top of the panel) and separated by capillary electrophoresis as described for (a). The dimer-to-monomer ratios (Dimer [%]) are shown at the bottom of the panel.

Techniques Used: Purification, Electrophoresis, Mutagenesis, Incubation, Affinity Magnetic Separation, Generated, Software

28) Product Images from "Thioredoxin A Active-Site Mutants Form Mixed Disulfide Dimers That Resemble Enzyme-Substrate Reaction Intermediates"

Article Title: Thioredoxin A Active-Site Mutants Form Mixed Disulfide Dimers That Resemble Enzyme-Substrate Reaction Intermediates

Journal: Journal of Molecular Biology

doi: 10.1016/j.jmb.2008.03.077

Redox states of BsTrxA monomers and dimers. (a) Purified His6-tagged BsTrxA proteins were separated by capillary electrophoresis using a 2100 Bioanalyzer (Agilent Technologies). WT, BsTrxA with wild-type active site; C29S, C29S single-mutant BsTrxA; C32S, C32S single-mutant BsTrxA; C29S–C32S, C29S–C32S double-mutant BsTrxA. To monitor the presence of free thiols in the purified BsTrxA proteins, samples were incubated in the presence or in the absence of 0.3 mM AMS (lanes marked + AMS or − AMS). To test whether the C29S and C32S dimers are formed by disulfide bonding, these proteins were incubated with 10 mM DTT (lanes marked + DTT). DTT was absent from all other samples. The image of the Bioanalyzer chromatogram was generated using the 2100 Expert Software package (Agilent Technologies). (b) C32S BsTrxA protein (∼ 2.5 μg) was reduced with increasing concentrations of DTT (shown on top of the panel) and separated by capillary electrophoresis as described for (a). The dimer-to-monomer ratios (Dimer [%]) are shown at the bottom of the panel.
Figure Legend Snippet: Redox states of BsTrxA monomers and dimers. (a) Purified His6-tagged BsTrxA proteins were separated by capillary electrophoresis using a 2100 Bioanalyzer (Agilent Technologies). WT, BsTrxA with wild-type active site; C29S, C29S single-mutant BsTrxA; C32S, C32S single-mutant BsTrxA; C29S–C32S, C29S–C32S double-mutant BsTrxA. To monitor the presence of free thiols in the purified BsTrxA proteins, samples were incubated in the presence or in the absence of 0.3 mM AMS (lanes marked + AMS or − AMS). To test whether the C29S and C32S dimers are formed by disulfide bonding, these proteins were incubated with 10 mM DTT (lanes marked + DTT). DTT was absent from all other samples. The image of the Bioanalyzer chromatogram was generated using the 2100 Expert Software package (Agilent Technologies). (b) C32S BsTrxA protein (∼ 2.5 μg) was reduced with increasing concentrations of DTT (shown on top of the panel) and separated by capillary electrophoresis as described for (a). The dimer-to-monomer ratios (Dimer [%]) are shown at the bottom of the panel.

Techniques Used: Purification, Electrophoresis, Mutagenesis, Incubation, Affinity Magnetic Separation, Generated, Software

29) Product Images from "Phase-defined complete sequencing of the HLA genes by next-generation sequencing"

Article Title: Phase-defined complete sequencing of the HLA genes by next-generation sequencing

Journal: BMC Genomics

doi: 10.1186/1471-2164-14-355

Size selection of the Nextera DNA libraries by agarose gel size selection. ( A ) Electropherogram of DNA library analyzed by 2100 Bioanalyzer. The library size of the Nextera DNA Sample Prep Kits was 150 bp to more than 10 kb (mean size: 902 bp). ( B ) Bioanalyzer electropherogram of a selected DNA library by cutting from the agarose gel. We selected large fragments with sizes ranging from 500 to 2,000 bp to remove short DNA fragments for effective HLA gene haplotype phasing. The size selection also determines an actual molar concentration for bridge PCR to generate clusters in flowcell, because DNA fragments with over 1.5 kb size are not efficiently amplified. The mean size of the selected fragments was 1,561 bp.
Figure Legend Snippet: Size selection of the Nextera DNA libraries by agarose gel size selection. ( A ) Electropherogram of DNA library analyzed by 2100 Bioanalyzer. The library size of the Nextera DNA Sample Prep Kits was 150 bp to more than 10 kb (mean size: 902 bp). ( B ) Bioanalyzer electropherogram of a selected DNA library by cutting from the agarose gel. We selected large fragments with sizes ranging from 500 to 2,000 bp to remove short DNA fragments for effective HLA gene haplotype phasing. The size selection also determines an actual molar concentration for bridge PCR to generate clusters in flowcell, because DNA fragments with over 1.5 kb size are not efficiently amplified. The mean size of the selected fragments was 1,561 bp.

Techniques Used: Selection, Agarose Gel Electrophoresis, Sample Prep, Concentration Assay, Bridge PCR, Amplification

30) Product Images from "Genotyping of KRAS Mutational Status by the In-Check Lab-on-Chip Platform"

Article Title: Genotyping of KRAS Mutational Status by the In-Check Lab-on-Chip Platform

Journal: Sensors (Basel, Switzerland)

doi: 10.3390/s18010131

Analysis performed by 2100-Bioanalyzer system of amplicons generated after PCR amplification onto Lab-on chip. The products obtained show an appropriate size difference to allow the discrimination and a good differentiation between the exons 2, 3, and 4.
Figure Legend Snippet: Analysis performed by 2100-Bioanalyzer system of amplicons generated after PCR amplification onto Lab-on chip. The products obtained show an appropriate size difference to allow the discrimination and a good differentiation between the exons 2, 3, and 4.

Techniques Used: Generated, Polymerase Chain Reaction, Amplification, Lab-on-a-Chip

31) Product Images from "Genotyping of KRAS Mutational Status by the In-Check Lab-on-Chip Platform"

Article Title: Genotyping of KRAS Mutational Status by the In-Check Lab-on-Chip Platform

Journal: Sensors (Basel, Switzerland)

doi: 10.3390/s18010131

Analysis performed by 2100-Bioanalyzer system of amplicons generated after PCR amplification onto Lab-on chip. The products obtained show an appropriate size difference to allow the discrimination and a good differentiation between the exons 2, 3, and 4.
Figure Legend Snippet: Analysis performed by 2100-Bioanalyzer system of amplicons generated after PCR amplification onto Lab-on chip. The products obtained show an appropriate size difference to allow the discrimination and a good differentiation between the exons 2, 3, and 4.

Techniques Used: Generated, Polymerase Chain Reaction, Amplification, Lab-on-a-Chip

32) Product Images from "Generation of Genetically Modified Mice using the CRISPR-Cas9 Genome-Editing System"

Article Title: Generation of Genetically Modified Mice using the CRISPR-Cas9 Genome-Editing System

Journal: Cold Spring Harbor protocols

doi: 10.1101/pdb.prot090704

Quality control of Cas9 mRNA in a 2100 Bioanalyzer and ssDNA donor oligo design A . Electrophoresis of in vitro transcribed Cas9 mRNA prior poly-polyadenylation and post poly-adenylation. B . Electropherogram of in vitro transcribed Cas9 mRNA prior poly-polyadenylation and post poly-adenylation. C . Representation of a sequence that can be targeted with a specific sgRNA. Insertion of EcoRI (as an example) adjacent to the PAM sequence will preclude the Cas9 nuclease from cutting again after the genome has been repaired by HDR.
Figure Legend Snippet: Quality control of Cas9 mRNA in a 2100 Bioanalyzer and ssDNA donor oligo design A . Electrophoresis of in vitro transcribed Cas9 mRNA prior poly-polyadenylation and post poly-adenylation. B . Electropherogram of in vitro transcribed Cas9 mRNA prior poly-polyadenylation and post poly-adenylation. C . Representation of a sequence that can be targeted with a specific sgRNA. Insertion of EcoRI (as an example) adjacent to the PAM sequence will preclude the Cas9 nuclease from cutting again after the genome has been repaired by HDR.

Techniques Used: Electrophoresis, In Vitro, Sequencing

33) Product Images from "Global Transcriptional Analysis of Virus-Host Interactions between Phage ϕ29 and Bacillus subtilis"

Article Title: Global Transcriptional Analysis of Virus-Host Interactions between Phage ϕ29 and Bacillus subtilis

Journal: Journal of Virology

doi: 10.1128/JVI.01245-16

Analysis of host growth, phage virions, and nucleic acid content throughout the infection cycle of phage ϕ29 in B. subtilis . (A) Phage-mediated lysis of bacterial cultures. Lysis was monitored by measuring the OD 600 values for samples taken at the indicated times after infection. (B) The ϕ29 virions were counted as PFU per ml of culture. One hundred-fold-diluted cultures were grown in LB medium containing magnesium at 37°C and then infected at an OD 600 of 0.45 with ϕ29 at a multiplicity of 5. (C) The amount of nucleic acid detected at the indicated times postinfection was quantified by using a 2100 Bioanalyzer (Agilent Technologies), and values are expressed as micrograms of nucleic acid per ml of culture. The graphics are representative of four independent experiments.
Figure Legend Snippet: Analysis of host growth, phage virions, and nucleic acid content throughout the infection cycle of phage ϕ29 in B. subtilis . (A) Phage-mediated lysis of bacterial cultures. Lysis was monitored by measuring the OD 600 values for samples taken at the indicated times after infection. (B) The ϕ29 virions were counted as PFU per ml of culture. One hundred-fold-diluted cultures were grown in LB medium containing magnesium at 37°C and then infected at an OD 600 of 0.45 with ϕ29 at a multiplicity of 5. (C) The amount of nucleic acid detected at the indicated times postinfection was quantified by using a 2100 Bioanalyzer (Agilent Technologies), and values are expressed as micrograms of nucleic acid per ml of culture. The graphics are representative of four independent experiments.

Techniques Used: Infection, Lysis

34) Product Images from "Arabidopsis RIBOSOMAL RNA PROCESSING7 Is Required for 18S rRNA Maturation"

Article Title: Arabidopsis RIBOSOMAL RNA PROCESSING7 Is Required for 18S rRNA Maturation

Journal: The Plant Cell

doi: 10.1105/tpc.18.00245

45S rDNA VAR Expression in smo4-3 and rrp7-1 . (A) and (B) Schematic representation of the 45S pre-rRNA (A) and its 3′-ETS polymorphic region (B) ). (C) to (E) PCR analysis of the relative abundance of 45S rDNA variants ( VAR1-VAR4 ) in reverse-transcribed RNA (C) , and genomic DNA (D) and (E) , from Col-0, smo4-3 , and rrp7-1 plants as indicated. Relative amounts of each 45S rDNA variant (E) were determined using the Agilent DNA 1000 kit on an Agilent 2100 Bioanalyzer. VAR4 was not detected. The ORNITHINE TRANSCARBAMYLASE ( OTC ) was used as an internal control in (C) .
Figure Legend Snippet: 45S rDNA VAR Expression in smo4-3 and rrp7-1 . (A) and (B) Schematic representation of the 45S pre-rRNA (A) and its 3′-ETS polymorphic region (B) ). (C) to (E) PCR analysis of the relative abundance of 45S rDNA variants ( VAR1-VAR4 ) in reverse-transcribed RNA (C) , and genomic DNA (D) and (E) , from Col-0, smo4-3 , and rrp7-1 plants as indicated. Relative amounts of each 45S rDNA variant (E) were determined using the Agilent DNA 1000 kit on an Agilent 2100 Bioanalyzer. VAR4 was not detected. The ORNITHINE TRANSCARBAMYLASE ( OTC ) was used as an internal control in (C) .

Techniques Used: Expressing, Polymerase Chain Reaction, Variant Assay

35) Product Images from "MicroRNA preparations from individual monogenean Gyrodactylus salaris-a comparison of six commercially available totalRNA extraction kits"

Article Title: MicroRNA preparations from individual monogenean Gyrodactylus salaris-a comparison of six commercially available totalRNA extraction kits

Journal: BMC Research Notes

doi: 10.1186/1756-0500-4-217

Boxplot summary of all data . Semilogarithmic boxplot breakdown of RNA yield for totalRNA, smallRNA and microRNA of all assessed totalRNA extraction kits for 1, 10 and 100 Gyrodactylus salaris specimens. RQI/RIN values of the totalRNA as determined by the instrument software following the Experion and 2100 Bioanalyzer electrophoresis systems are depicted at the bottom. Boxes indicate the 25-75% intervals, squares the mean, and whiskers the standard deviation, where this lies outside the 25-75% interval.
Figure Legend Snippet: Boxplot summary of all data . Semilogarithmic boxplot breakdown of RNA yield for totalRNA, smallRNA and microRNA of all assessed totalRNA extraction kits for 1, 10 and 100 Gyrodactylus salaris specimens. RQI/RIN values of the totalRNA as determined by the instrument software following the Experion and 2100 Bioanalyzer electrophoresis systems are depicted at the bottom. Boxes indicate the 25-75% intervals, squares the mean, and whiskers the standard deviation, where this lies outside the 25-75% interval.

Techniques Used: Software, Electrophoresis, Standard Deviation

36) Product Images from "Retinoid Expression in Onchocercal Skin Disease: Pilot Study"

Article Title: Retinoid Expression in Onchocercal Skin Disease: Pilot Study

Journal: Infectious Diseases

doi: 10.1177/1178633617731741

RNA quality results from the 2100 Bioanalyzer.
Figure Legend Snippet: RNA quality results from the 2100 Bioanalyzer.

Techniques Used:

37) Product Images from "MicroRNA preparations from individual monogenean Gyrodactylus salaris-a comparison of six commercially available totalRNA extraction kits"

Article Title: MicroRNA preparations from individual monogenean Gyrodactylus salaris-a comparison of six commercially available totalRNA extraction kits

Journal: BMC Research Notes

doi: 10.1186/1756-0500-4-217

Boxplot summary of all data . Semilogarithmic boxplot breakdown of RNA yield for totalRNA, smallRNA and microRNA of all assessed totalRNA extraction kits for 1, 10 and 100 Gyrodactylus salaris specimens. RQI/RIN values of the totalRNA as determined by the instrument software following the Experion and 2100 Bioanalyzer electrophoresis systems are depicted at the bottom. Boxes indicate the 25-75% intervals, squares the mean, and whiskers the standard deviation, where this lies outside the 25-75% interval.
Figure Legend Snippet: Boxplot summary of all data . Semilogarithmic boxplot breakdown of RNA yield for totalRNA, smallRNA and microRNA of all assessed totalRNA extraction kits for 1, 10 and 100 Gyrodactylus salaris specimens. RQI/RIN values of the totalRNA as determined by the instrument software following the Experion and 2100 Bioanalyzer electrophoresis systems are depicted at the bottom. Boxes indicate the 25-75% intervals, squares the mean, and whiskers the standard deviation, where this lies outside the 25-75% interval.

Techniques Used: Software, Electrophoresis, Standard Deviation

38) Product Images from "Method Enabling Gene Expression Studies of Pathogens in a Complex Food Matrix ▿"

Article Title: Method Enabling Gene Expression Studies of Pathogens in a Complex Food Matrix ▿

Journal: Applied and Environmental Microbiology

doi: 10.1128/AEM.05471-11

Electropherograms (created using an Agilent 2100 Bioanalyzer) showing integrity of RNA samples extracted from an E. coli mixed culture inoculated into minced beef (A) and an E. coli mixed culture in LB broth (B). FU, fluorescence units.
Figure Legend Snippet: Electropherograms (created using an Agilent 2100 Bioanalyzer) showing integrity of RNA samples extracted from an E. coli mixed culture inoculated into minced beef (A) and an E. coli mixed culture in LB broth (B). FU, fluorescence units.

Techniques Used: Fluorescence

Related Articles

Methylation Sequencing:

Article Title: A streamlined method for analysing genome-wide DNA methylation patterns from low amounts of FFPE DNA
Article Snippet: .. Preparation of libraries for reduced representation bisulfite sequencing (RRBS) or other genome-wide methylation applications (from FFPE samples) The quality of the RRBS libraries prepared from melanoma FFPE slices was assessed on a 2100 Bioanalyzer (Agilent Technologies) using the high sensitivity DNA chip. .. Bioanalyzer analysis of two representative FFPE derived RRBS libraries are shown in Fig. .

Centrifugation:

Article Title: Effect of lesion proximity on the regenerative response of long descending propriospinal neurons after spinal transection injury
Article Snippet: RNA was purified using the RNeasy Mini kit (Qiagen; Valencia, CA), eluted in 30 mL nuclease-free water and concentrated down to 10 mL by vacuum centrifugation. .. Quality of the RNA extraction was determined utilizing a 2100 bioanalyzer (Agilent Technologies; Santa Clara, CA) which provided an RNA Integrity Number (RIN), and corresponding pseudo gel (Fig. ).

Article Title: Effects of Acute Aerobic Exercise on Rats Serum Extracellular Vesicles Diameter, Concentration and Small RNAs Content
Article Snippet: The columns were washed using 100 μl of 1BF washing solution and centrifugation for 30 s at 11,000 × g . .. The RNA was eluted from the column after washing it twice with 50 μl of RNase-free water, incubation for 1 min at room temperature and centrifuging for 1 min at 11,000 × g. The 2100 Bioanalyzer (Agilent) small RNA chip was used to analyze concentration and size from the purified small RNA samples.

Amplification:

Article Title: Identification of deleterious rare variants in MTTP, PNPLA3, and TM6SF2 in Japanese males and association studies with NAFLD
Article Snippet: .. The amplicon sizes of RT-PCR products were measured by a 2100 BioAnalyzer (Agilent Technologies, Santa Clara, CA). .. MTP activity was measured using a fluorometric assay kit (Sigma-Aldrich, St. Louis, MO) according to the manufacturer’s instructions.

Article Title: VR09 Cell Line: An EBV-Positive Lymphoblastoid Cell Line with In Vivo Characteristics of Diffuse Large B Cell Lymphoma of Activated B-Cell Type
Article Snippet: To verify that EBV infection of VR09 derived directly from the patient, the presence of EBV gene RPMS1 was detected by PCR amplification on both DNA from the patient and VR09 cell line. .. PCR products were analyzed on a 2100 Bioanalyzer (Agilent Technologies, Waldbronn, Germany), using the Agilent DNA1000 chip and reagents.

Polymerase Chain Reaction:

Article Title: Effect of lesion proximity on the regenerative response of long descending propriospinal neurons after spinal transection injury
Article Snippet: RNA purification Laser-dissected neurons were collected directly into a nuclease-free PCR tube cap, containing 30 mL RLT lysis buffer (Qiagen; Valencia, CA) with freshly-added 1% 2-mercaptoethanol (Sigma Aldrich; St. Louis, MO). .. Quality of the RNA extraction was determined utilizing a 2100 bioanalyzer (Agilent Technologies; Santa Clara, CA) which provided an RNA Integrity Number (RIN), and corresponding pseudo gel (Fig. ).

Article Title: The characterization of four gene expression analysis in circulating tumor cells made by Multiplex-PCR from the AdnaTest kit on the lab-on-a-chip Agilent DNA 1000 platform
Article Snippet: .. Subjects The study describes the PCR fragment size and concentration determination performed with the commercially available Agilent DNA 1000 reagent kit (Agilent Technologies, USA, Santa Clara) on the 2100 Bioanalyzer (Agilent Technologies, USA, Santa Clara). ..

Article Title: Identification of deleterious rare variants in MTTP, PNPLA3, and TM6SF2 in Japanese males and association studies with NAFLD
Article Snippet: The PCR fragment of introns 6–8 of TM6SF2 from the c.712-2A > T mutation carrier and introns 4–5 of PNPLA3 from the rs148469440 variant carrier were inserted into intron 1 of ACKR1 and TRIB2 , respectively. .. The amplicon sizes of RT-PCR products were measured by a 2100 BioAnalyzer (Agilent Technologies, Santa Clara, CA).

Article Title: VR09 Cell Line: An EBV-Positive Lymphoblastoid Cell Line with In Vivo Characteristics of Diffuse Large B Cell Lymphoma of Activated B-Cell Type
Article Snippet: .. PCR products were analyzed on a 2100 Bioanalyzer (Agilent Technologies, Waldbronn, Germany), using the Agilent DNA1000 chip and reagents. .. Electronic data were analyzed using the manufacturer’s software (Agilent 2100 Expert).

Article Title: Generating Exome Enriched Sequencing Libraries from Formalin-Fixed, Paraffin-Embedded Tissue DNA for Next Generation Sequencing
Article Snippet: .. Universal Blocker Oligonucleotide (Integrated DNA Technology) – See Reagents and Solutions Indexed Blocker Oligonucleotide (Integrated DNA Technology) – See Reagents and Solutions Nuclease Free Water (ThermoFisher Scientific; pn: AM9930) SureSelect XT Human All Exon Kit (Agilent; pn:5190-8863) SureSelect Hyb #1, 2, 3, 4 SureSelect Block # 1, 2 RNase Block SureSelect Human All Exon Capture Sure Select Binding Buffer Sure Select Wash Buffer I Sure Select Wash Buffer II Dynabeads My One Streptavidin T1 (ThermoFisher Scientific; pn: 65602) HiFi HotStart ReadyMix (2×) (KapaBiosystems; pn: KK2612) PCR Primer 1 (Integrated DNA Technology) – See Reagents and Solutions PCR Primer 2 (Integrated DNA Technology) – See Reagents and Solutions Agencourt Ampure XP (Beckman Coulter; pn: ) Ethanol 100% molecular biology grade Elution Buffer (Qiagen; pn: 19086) 2100 BioAnalyzer (Agilent) Vacuum concentrator MicroAmp Optical 8-Cap Strips (ThermoFisher Scientific; pn: 4323032) High Sensitivity DNA Kit (Agilent; 5067-4626) MicroAmp Optical 96 well Reaction Plate (ThermoFisher Scientific; pn: N8010560) MicroAmp Clear Adhesive Film (ThermoFisher Scientific; pn: 4306311) DynaMag-2 Magnet (Invitrogen; pn: 12321D) DynaMag-96 Side Skirted Plate Magnet (Invitrogen; pn: 12027 or 12331D) DNA LoBind Tubes (Eppendorf; pn: 22431021) Tube Rocker BD Clay Adams Nutator Mixer (BD Diagnostics; pn: 421105) Torrey Pines Echotherm Heat Block (Torrey Pines) ..

Construct:

Article Title: Identification of deleterious rare variants in MTTP, PNPLA3, and TM6SF2 in Japanese males and association studies with NAFLD
Article Snippet: After validation of the construct sequences, the mini-genes were inserted under control of the CMV promoter into the pCR3 vector and transfected into Huh-7 cells. .. The amplicon sizes of RT-PCR products were measured by a 2100 BioAnalyzer (Agilent Technologies, Santa Clara, CA).

Electrophoresis:

Article Title: A highly effective and versatile technology for the isolation of RNAs from grapevines and other woody perennials for use in virus diagnostics
Article Snippet: RNA integrity was verified based on the 28S and 18S rRNA bands after electrophoresis on 1.5 % formaldehyde–agarose gel, stained with ethidium bromide and visualization with UV light. .. The integrity and size distribution of purified RNAs were also evaluated with a 2100 Bioanalyzer (Agilent Technologies, Inc. Santa Clara, CA) equipped with an RNA Nano chip.

Article Title: The Effect of Formaldehyde Fixation on RNA
Article Snippet: .. The compositions of the total HeLa RNA and RNA 50mer preparations were characterized by capillary electrophoresis using a 2100 bioanalyzer (Agilent Technologies, Santa Clara, CA). .. Each RNA solution, 1 μL (0.5 to 1 μg per well), was loaded into a nano total RNA chip (Agilent 6000) and run according to the manufacturer's instructions.

Incubation:

Article Title: Effects of Acute Aerobic Exercise on Rats Serum Extracellular Vesicles Diameter, Concentration and Small RNAs Content
Article Snippet: .. The RNA was eluted from the column after washing it twice with 50 μl of RNase-free water, incubation for 1 min at room temperature and centrifuging for 1 min at 11,000 × g. The 2100 Bioanalyzer (Agilent) small RNA chip was used to analyze concentration and size from the purified small RNA samples. ..

Formalin-fixed Paraffin-Embedded:

Article Title: A streamlined method for analysing genome-wide DNA methylation patterns from low amounts of FFPE DNA
Article Snippet: .. Preparation of libraries for reduced representation bisulfite sequencing (RRBS) or other genome-wide methylation applications (from FFPE samples) The quality of the RRBS libraries prepared from melanoma FFPE slices was assessed on a 2100 Bioanalyzer (Agilent Technologies) using the high sensitivity DNA chip. .. Bioanalyzer analysis of two representative FFPE derived RRBS libraries are shown in Fig. .

Activity Assay:

Article Title: RNase L Interacts with Filamin A To Regulate Actin Dynamics and Barrier Function for Viral Entry
Article Snippet: .. RNAs (2 µg) were separated on RNA chips and analyzed with a 2100 Bioanalyzer (Agilent Technologies) to monitor characteristic rRNA cleavage products generated by RNase L activity as described previously ( ). ..

Article Title: Identification of deleterious rare variants in MTTP, PNPLA3, and TM6SF2 in Japanese males and association studies with NAFLD
Article Snippet: The amplicon sizes of RT-PCR products were measured by a 2100 BioAnalyzer (Agilent Technologies, Santa Clara, CA). .. MTP activity was measured using a fluorometric assay kit (Sigma-Aldrich, St. Louis, MO) according to the manufacturer’s instructions.

Cell Culture:

Article Title: VR09 Cell Line: An EBV-Positive Lymphoblastoid Cell Line with In Vivo Characteristics of Diffuse Large B Cell Lymphoma of Activated B-Cell Type
Article Snippet: EBV Status Evaluation The presence of EBV was assessed on both cells cultured in vitro and cells obtained from the tumoral mass developing after in vivo injection of VR09 cell line. .. PCR products were analyzed on a 2100 Bioanalyzer (Agilent Technologies, Waldbronn, Germany), using the Agilent DNA1000 chip and reagents.

Genome Wide:

Article Title: A streamlined method for analysing genome-wide DNA methylation patterns from low amounts of FFPE DNA
Article Snippet: .. Preparation of libraries for reduced representation bisulfite sequencing (RRBS) or other genome-wide methylation applications (from FFPE samples) The quality of the RRBS libraries prepared from melanoma FFPE slices was assessed on a 2100 Bioanalyzer (Agilent Technologies) using the high sensitivity DNA chip. .. Bioanalyzer analysis of two representative FFPE derived RRBS libraries are shown in Fig. .

Derivative Assay:

Article Title: A streamlined method for analysing genome-wide DNA methylation patterns from low amounts of FFPE DNA
Article Snippet: Preparation of libraries for reduced representation bisulfite sequencing (RRBS) or other genome-wide methylation applications (from FFPE samples) The quality of the RRBS libraries prepared from melanoma FFPE slices was assessed on a 2100 Bioanalyzer (Agilent Technologies) using the high sensitivity DNA chip. .. Bioanalyzer analysis of two representative FFPE derived RRBS libraries are shown in Fig. .

Article Title: VR09 Cell Line: An EBV-Positive Lymphoblastoid Cell Line with In Vivo Characteristics of Diffuse Large B Cell Lymphoma of Activated B-Cell Type
Article Snippet: To verify that EBV infection of VR09 derived directly from the patient, the presence of EBV gene RPMS1 was detected by PCR amplification on both DNA from the patient and VR09 cell line. .. PCR products were analyzed on a 2100 Bioanalyzer (Agilent Technologies, Waldbronn, Germany), using the Agilent DNA1000 chip and reagents.

Hybridization:

Article Title: VR09 Cell Line: An EBV-Positive Lymphoblastoid Cell Line with In Vivo Characteristics of Diffuse Large B Cell Lymphoma of Activated B-Cell Type
Article Snippet: Epstein-Barr virus-encoded RNA (EBER) hybridization was performed on cytoinclusion by means of specific fluorescein-conjugated EBER probes (Bond ISH EBER Probe, Vision-Biosystem, Menarini, Florence, Italy). .. PCR products were analyzed on a 2100 Bioanalyzer (Agilent Technologies, Waldbronn, Germany), using the Agilent DNA1000 chip and reagents.

Transfection:

Article Title: RNase L Interacts with Filamin A To Regulate Actin Dynamics and Barrier Function for Viral Entry
Article Snippet: At the indicated times, the total RNA was isolated from transfected cells using Trizol reagent (Invitrogen/Life Technologies) and quantitated by measuring absorbance at 260 nm using a NanoDrop spectrophotometer (Thermo Scientific, Wilmington, DE). .. RNAs (2 µg) were separated on RNA chips and analyzed with a 2100 Bioanalyzer (Agilent Technologies) to monitor characteristic rRNA cleavage products generated by RNase L activity as described previously ( ).

Article Title: Identification of deleterious rare variants in MTTP, PNPLA3, and TM6SF2 in Japanese males and association studies with NAFLD
Article Snippet: Twenty-four hours after transfection, total RNA was extracted from the transfected cells, and the corresponding cDNA was used as a template for RT-PCR. .. The amplicon sizes of RT-PCR products were measured by a 2100 BioAnalyzer (Agilent Technologies, Santa Clara, CA).

Ligation:

Article Title: Generating Exome Enriched Sequencing Libraries from Formalin-Fixed, Paraffin-Embedded Tissue DNA for Next Generation Sequencing
Article Snippet: .. Hyper Prep Kit (KapaBiosystems; pn: KK8504) End Repair/A Tailing Buffer End Repair/A Tailing Enzyme Ligation Buffer Kapa T4 DNA Ligase Kapa HiFi Master Mix Kapa Primer Premix Indexed Adapter oligonucleotide (Integrated DNA Technology) – See Reagents and Solutions 20% PEG/2.5M NaCl solution – See Reagents and Solutions Ethanol 100% molecular biology grade Nuclease Free Water (ThermoFisher Scientific; pn: AM9930) Agencourt Ampure XP (Beckman Coulter; pn: ) MicroAmp Optical 96 well Reaction Plate (ThermoFisher Scientific; pn: N8010560) MicroAmp Clear Adhesive Film (ThermoFisher Scientific; pn: 4306311) 2100 BioAnalyzer (Agilent) Elution Buffer (Qiagen; pn: 19086) High Sensitivity DNA Kit (Agilent; 5067-4626) Vortex Centrifuge Thermal cycler DynaMag-96 Side Skirted Plate Magnet (Invitrogen; pn:12027 or 12331D) ..

Infection:

Article Title: VR09 Cell Line: An EBV-Positive Lymphoblastoid Cell Line with In Vivo Characteristics of Diffuse Large B Cell Lymphoma of Activated B-Cell Type
Article Snippet: To verify that EBV infection of VR09 derived directly from the patient, the presence of EBV gene RPMS1 was detected by PCR amplification on both DNA from the patient and VR09 cell line. .. PCR products were analyzed on a 2100 Bioanalyzer (Agilent Technologies, Waldbronn, Germany), using the Agilent DNA1000 chip and reagents.

Generated:

Article Title: RNase L Interacts with Filamin A To Regulate Actin Dynamics and Barrier Function for Viral Entry
Article Snippet: .. RNAs (2 µg) were separated on RNA chips and analyzed with a 2100 Bioanalyzer (Agilent Technologies) to monitor characteristic rRNA cleavage products generated by RNase L activity as described previously ( ). ..

Sequencing:

Article Title: High quality RNA extraction from Maqui berry for its application in next-generation sequencing
Article Snippet: The quality of these libraries was assessed with a DNA 1000 kit in a 2100 Bioanalyzer (Agilent Technologies). .. The libraries were ready to be sequenced using next-generation sequencing platform and was achieved a high quality de novo sequence assembly.

Article Title: Tropomyosin isoforms differentially affect muscle contractility in the head and body regions of the nematode Caenorhabditis elegans
Article Snippet: Paragraph title: RT-PCR and sequencing ... RT-PCR products were analyzed by using a 2100 BioAnalyzer (Agilent Technologies).

Article Title: VR09 Cell Line: An EBV-Positive Lymphoblastoid Cell Line with In Vivo Characteristics of Diffuse Large B Cell Lymphoma of Activated B-Cell Type
Article Snippet: EBER sequence was detected by anti-fluorescin antibody associated with a sensitive ‘Bond polymer Refine’ chromogenic detection system in an automated immunostainer (Bondmax, Vision-Biosystem, Menarini, Florence, Italy). .. PCR products were analyzed on a 2100 Bioanalyzer (Agilent Technologies, Waldbronn, Germany), using the Agilent DNA1000 chip and reagents.

Sonication:

Article Title: Identification of deleterious rare variants in MTTP, PNPLA3, and TM6SF2 in Japanese males and association studies with NAFLD
Article Snippet: The amplicon sizes of RT-PCR products were measured by a 2100 BioAnalyzer (Agilent Technologies, Santa Clara, CA). .. Twenty-four hours after the transfection, cells were harvested in 400 μL buffer containing protease inhibitors and homogenized by sonication.

Injection:

Article Title: VR09 Cell Line: An EBV-Positive Lymphoblastoid Cell Line with In Vivo Characteristics of Diffuse Large B Cell Lymphoma of Activated B-Cell Type
Article Snippet: EBV Status Evaluation The presence of EBV was assessed on both cells cultured in vitro and cells obtained from the tumoral mass developing after in vivo injection of VR09 cell line. .. PCR products were analyzed on a 2100 Bioanalyzer (Agilent Technologies, Waldbronn, Germany), using the Agilent DNA1000 chip and reagents.

Binding Assay:

Article Title: Generating Exome Enriched Sequencing Libraries from Formalin-Fixed, Paraffin-Embedded Tissue DNA for Next Generation Sequencing
Article Snippet: .. Universal Blocker Oligonucleotide (Integrated DNA Technology) – See Reagents and Solutions Indexed Blocker Oligonucleotide (Integrated DNA Technology) – See Reagents and Solutions Nuclease Free Water (ThermoFisher Scientific; pn: AM9930) SureSelect XT Human All Exon Kit (Agilent; pn:5190-8863) SureSelect Hyb #1, 2, 3, 4 SureSelect Block # 1, 2 RNase Block SureSelect Human All Exon Capture Sure Select Binding Buffer Sure Select Wash Buffer I Sure Select Wash Buffer II Dynabeads My One Streptavidin T1 (ThermoFisher Scientific; pn: 65602) HiFi HotStart ReadyMix (2×) (KapaBiosystems; pn: KK2612) PCR Primer 1 (Integrated DNA Technology) – See Reagents and Solutions PCR Primer 2 (Integrated DNA Technology) – See Reagents and Solutions Agencourt Ampure XP (Beckman Coulter; pn: ) Ethanol 100% molecular biology grade Elution Buffer (Qiagen; pn: 19086) 2100 BioAnalyzer (Agilent) Vacuum concentrator MicroAmp Optical 8-Cap Strips (ThermoFisher Scientific; pn: 4323032) High Sensitivity DNA Kit (Agilent; 5067-4626) MicroAmp Optical 96 well Reaction Plate (ThermoFisher Scientific; pn: N8010560) MicroAmp Clear Adhesive Film (ThermoFisher Scientific; pn: 4306311) DynaMag-2 Magnet (Invitrogen; pn: 12321D) DynaMag-96 Side Skirted Plate Magnet (Invitrogen; pn: 12027 or 12331D) DNA LoBind Tubes (Eppendorf; pn: 22431021) Tube Rocker BD Clay Adams Nutator Mixer (BD Diagnostics; pn: 421105) Torrey Pines Echotherm Heat Block (Torrey Pines) ..

In Vivo:

Article Title: VR09 Cell Line: An EBV-Positive Lymphoblastoid Cell Line with In Vivo Characteristics of Diffuse Large B Cell Lymphoma of Activated B-Cell Type
Article Snippet: EBV Status Evaluation The presence of EBV was assessed on both cells cultured in vitro and cells obtained from the tumoral mass developing after in vivo injection of VR09 cell line. .. PCR products were analyzed on a 2100 Bioanalyzer (Agilent Technologies, Waldbronn, Germany), using the Agilent DNA1000 chip and reagents.

Fluorescence:

Article Title: Identification of deleterious rare variants in MTTP, PNPLA3, and TM6SF2 in Japanese males and association studies with NAFLD
Article Snippet: The stained cells were visualized under a fluorescence microscope, and the ratios of GFP-PNPLA3 on lipid droplets to total GFP fluorescence and LipidTOX fluorescence in GFP-expressing cells were measured from 10 randomly selected fields using the associated software (KEYENCE Japan, Osaka, Japan). .. The amplicon sizes of RT-PCR products were measured by a 2100 BioAnalyzer (Agilent Technologies, Santa Clara, CA).

Methylation:

Article Title: A streamlined method for analysing genome-wide DNA methylation patterns from low amounts of FFPE DNA
Article Snippet: .. Preparation of libraries for reduced representation bisulfite sequencing (RRBS) or other genome-wide methylation applications (from FFPE samples) The quality of the RRBS libraries prepared from melanoma FFPE slices was assessed on a 2100 Bioanalyzer (Agilent Technologies) using the high sensitivity DNA chip. .. Bioanalyzer analysis of two representative FFPE derived RRBS libraries are shown in Fig. .

Mutagenesis:

Article Title: Identification of deleterious rare variants in MTTP, PNPLA3, and TM6SF2 in Japanese males and association studies with NAFLD
Article Snippet: The PCR fragment of introns 6–8 of TM6SF2 from the c.712-2A > T mutation carrier and introns 4–5 of PNPLA3 from the rs148469440 variant carrier were inserted into intron 1 of ACKR1 and TRIB2 , respectively. .. The amplicon sizes of RT-PCR products were measured by a 2100 BioAnalyzer (Agilent Technologies, Santa Clara, CA).

Isolation:

Article Title: RNase L Interacts with Filamin A To Regulate Actin Dynamics and Barrier Function for Viral Entry
Article Snippet: At the indicated times, the total RNA was isolated from transfected cells using Trizol reagent (Invitrogen/Life Technologies) and quantitated by measuring absorbance at 260 nm using a NanoDrop spectrophotometer (Thermo Scientific, Wilmington, DE). .. RNAs (2 µg) were separated on RNA chips and analyzed with a 2100 Bioanalyzer (Agilent Technologies) to monitor characteristic rRNA cleavage products generated by RNase L activity as described previously ( ).

Article Title: A highly effective and versatile technology for the isolation of RNAs from grapevines and other woody perennials for use in virus diagnostics
Article Snippet: The integrity and size distribution of purified RNAs were also evaluated with a 2100 Bioanalyzer (Agilent Technologies, Inc. Santa Clara, CA) equipped with an RNA Nano chip. .. Small RNAs and miRNAs from the total RNA samples isolated from young grapevine leaves with the five commercial kits were analyzed by the Agilent Bioanalyzer equipped with Small RNA Analysis kit (Agilent Technologies, Inc.) and the yields of small RNAs and miRNAs were calculated using the software Bio sizing version B.02.08.

Article Title: Comprehensive Investigation of miRNome Identifies Novel Candidate miRNA-mRNA Interactions Implicated in T-Cell Acute Lymphoblastic Leukemia
Article Snippet: Paragraph title: RNA Isolation and Quality Control ... RNA integrity was determined with 4200 Tapestation using High Sensitivity RNA ScreenTape (Agilent Technologies) and 2100 Bioanalyzer using RNA 6000 Nano Assay (Agilent Technologies).

RNA Extraction:

Article Title: Effect of lesion proximity on the regenerative response of long descending propriospinal neurons after spinal transection injury
Article Snippet: .. Quality of the RNA extraction was determined utilizing a 2100 bioanalyzer (Agilent Technologies; Santa Clara, CA) which provided an RNA Integrity Number (RIN), and corresponding pseudo gel (Fig. ). ..

Microscopy:

Article Title: Identification of deleterious rare variants in MTTP, PNPLA3, and TM6SF2 in Japanese males and association studies with NAFLD
Article Snippet: The stained cells were visualized under a fluorescence microscope, and the ratios of GFP-PNPLA3 on lipid droplets to total GFP fluorescence and LipidTOX fluorescence in GFP-expressing cells were measured from 10 randomly selected fields using the associated software (KEYENCE Japan, Osaka, Japan). .. The amplicon sizes of RT-PCR products were measured by a 2100 BioAnalyzer (Agilent Technologies, Santa Clara, CA).

Purification:

Article Title: Effect of lesion proximity on the regenerative response of long descending propriospinal neurons after spinal transection injury
Article Snippet: Paragraph title: RNA purification ... Quality of the RNA extraction was determined utilizing a 2100 bioanalyzer (Agilent Technologies; Santa Clara, CA) which provided an RNA Integrity Number (RIN), and corresponding pseudo gel (Fig. ).

Article Title: Effects of Acute Aerobic Exercise on Rats Serum Extracellular Vesicles Diameter, Concentration and Small RNAs Content
Article Snippet: .. The RNA was eluted from the column after washing it twice with 50 μl of RNase-free water, incubation for 1 min at room temperature and centrifuging for 1 min at 11,000 × g. The 2100 Bioanalyzer (Agilent) small RNA chip was used to analyze concentration and size from the purified small RNA samples. ..

Article Title: A highly effective and versatile technology for the isolation of RNAs from grapevines and other woody perennials for use in virus diagnostics
Article Snippet: .. The integrity and size distribution of purified RNAs were also evaluated with a 2100 Bioanalyzer (Agilent Technologies, Inc. Santa Clara, CA) equipped with an RNA Nano chip. .. Small RNAs and miRNAs from the total RNA samples isolated from young grapevine leaves with the five commercial kits were analyzed by the Agilent Bioanalyzer equipped with Small RNA Analysis kit (Agilent Technologies, Inc.) and the yields of small RNAs and miRNAs were calculated using the software Bio sizing version B.02.08.

Article Title: Comprehensive Investigation of miRNome Identifies Novel Candidate miRNA-mRNA Interactions Implicated in T-Cell Acute Lymphoblastic Leukemia
Article Snippet: RNA isolates were DNase treated and purified with use of RNA Clean and Concentrator Kit (Zymo Research). .. RNA integrity was determined with 4200 Tapestation using High Sensitivity RNA ScreenTape (Agilent Technologies) and 2100 Bioanalyzer using RNA 6000 Nano Assay (Agilent Technologies).

Reverse Transcription Polymerase Chain Reaction:

Article Title: Identification of deleterious rare variants in MTTP, PNPLA3, and TM6SF2 in Japanese males and association studies with NAFLD
Article Snippet: .. The amplicon sizes of RT-PCR products were measured by a 2100 BioAnalyzer (Agilent Technologies, Santa Clara, CA). .. MTP activity was measured using a fluorometric assay kit (Sigma-Aldrich, St. Louis, MO) according to the manufacturer’s instructions.

Article Title: Tropomyosin isoforms differentially affect muscle contractility in the head and body regions of the nematode Caenorhabditis elegans
Article Snippet: .. RT-PCR products were analyzed by using a 2100 BioAnalyzer (Agilent Technologies). ..

Blocking Assay:

Article Title: Generating Exome Enriched Sequencing Libraries from Formalin-Fixed, Paraffin-Embedded Tissue DNA for Next Generation Sequencing
Article Snippet: .. Universal Blocker Oligonucleotide (Integrated DNA Technology) – See Reagents and Solutions Indexed Blocker Oligonucleotide (Integrated DNA Technology) – See Reagents and Solutions Nuclease Free Water (ThermoFisher Scientific; pn: AM9930) SureSelect XT Human All Exon Kit (Agilent; pn:5190-8863) SureSelect Hyb #1, 2, 3, 4 SureSelect Block # 1, 2 RNase Block SureSelect Human All Exon Capture Sure Select Binding Buffer Sure Select Wash Buffer I Sure Select Wash Buffer II Dynabeads My One Streptavidin T1 (ThermoFisher Scientific; pn: 65602) HiFi HotStart ReadyMix (2×) (KapaBiosystems; pn: KK2612) PCR Primer 1 (Integrated DNA Technology) – See Reagents and Solutions PCR Primer 2 (Integrated DNA Technology) – See Reagents and Solutions Agencourt Ampure XP (Beckman Coulter; pn: ) Ethanol 100% molecular biology grade Elution Buffer (Qiagen; pn: 19086) 2100 BioAnalyzer (Agilent) Vacuum concentrator MicroAmp Optical 8-Cap Strips (ThermoFisher Scientific; pn: 4323032) High Sensitivity DNA Kit (Agilent; 5067-4626) MicroAmp Optical 96 well Reaction Plate (ThermoFisher Scientific; pn: N8010560) MicroAmp Clear Adhesive Film (ThermoFisher Scientific; pn: 4306311) DynaMag-2 Magnet (Invitrogen; pn: 12321D) DynaMag-96 Side Skirted Plate Magnet (Invitrogen; pn: 12027 or 12331D) DNA LoBind Tubes (Eppendorf; pn: 22431021) Tube Rocker BD Clay Adams Nutator Mixer (BD Diagnostics; pn: 421105) Torrey Pines Echotherm Heat Block (Torrey Pines) ..

Lysis:

Article Title: Effect of lesion proximity on the regenerative response of long descending propriospinal neurons after spinal transection injury
Article Snippet: RNA purification Laser-dissected neurons were collected directly into a nuclease-free PCR tube cap, containing 30 mL RLT lysis buffer (Qiagen; Valencia, CA) with freshly-added 1% 2-mercaptoethanol (Sigma Aldrich; St. Louis, MO). .. Quality of the RNA extraction was determined utilizing a 2100 bioanalyzer (Agilent Technologies; Santa Clara, CA) which provided an RNA Integrity Number (RIN), and corresponding pseudo gel (Fig. ).

Chromatin Immunoprecipitation:

Article Title: A streamlined method for analysing genome-wide DNA methylation patterns from low amounts of FFPE DNA
Article Snippet: .. Preparation of libraries for reduced representation bisulfite sequencing (RRBS) or other genome-wide methylation applications (from FFPE samples) The quality of the RRBS libraries prepared from melanoma FFPE slices was assessed on a 2100 Bioanalyzer (Agilent Technologies) using the high sensitivity DNA chip. .. Bioanalyzer analysis of two representative FFPE derived RRBS libraries are shown in Fig. .

Article Title: Effects of Acute Aerobic Exercise on Rats Serum Extracellular Vesicles Diameter, Concentration and Small RNAs Content
Article Snippet: .. The RNA was eluted from the column after washing it twice with 50 μl of RNase-free water, incubation for 1 min at room temperature and centrifuging for 1 min at 11,000 × g. The 2100 Bioanalyzer (Agilent) small RNA chip was used to analyze concentration and size from the purified small RNA samples. ..

Article Title: A highly effective and versatile technology for the isolation of RNAs from grapevines and other woody perennials for use in virus diagnostics
Article Snippet: .. The integrity and size distribution of purified RNAs were also evaluated with a 2100 Bioanalyzer (Agilent Technologies, Inc. Santa Clara, CA) equipped with an RNA Nano chip. .. Small RNAs and miRNAs from the total RNA samples isolated from young grapevine leaves with the five commercial kits were analyzed by the Agilent Bioanalyzer equipped with Small RNA Analysis kit (Agilent Technologies, Inc.) and the yields of small RNAs and miRNAs were calculated using the software Bio sizing version B.02.08.

Article Title: VR09 Cell Line: An EBV-Positive Lymphoblastoid Cell Line with In Vivo Characteristics of Diffuse Large B Cell Lymphoma of Activated B-Cell Type
Article Snippet: .. PCR products were analyzed on a 2100 Bioanalyzer (Agilent Technologies, Waldbronn, Germany), using the Agilent DNA1000 chip and reagents. .. Electronic data were analyzed using the manufacturer’s software (Agilent 2100 Expert).

Article Title: The Effect of Formaldehyde Fixation on RNA
Article Snippet: The compositions of the total HeLa RNA and RNA 50mer preparations were characterized by capillary electrophoresis using a 2100 bioanalyzer (Agilent Technologies, Santa Clara, CA). .. Each RNA solution, 1 μL (0.5 to 1 μg per well), was loaded into a nano total RNA chip (Agilent 6000) and run according to the manufacturer's instructions.

In Situ Hybridization:

Article Title: VR09 Cell Line: An EBV-Positive Lymphoblastoid Cell Line with In Vivo Characteristics of Diffuse Large B Cell Lymphoma of Activated B-Cell Type
Article Snippet: Epstein-Barr virus-encoded RNA (EBER) hybridization was performed on cytoinclusion by means of specific fluorescein-conjugated EBER probes (Bond ISH EBER Probe, Vision-Biosystem, Menarini, Florence, Italy). .. PCR products were analyzed on a 2100 Bioanalyzer (Agilent Technologies, Waldbronn, Germany), using the Agilent DNA1000 chip and reagents.

Plasmid Preparation:

Article Title: Identification of deleterious rare variants in MTTP, PNPLA3, and TM6SF2 in Japanese males and association studies with NAFLD
Article Snippet: After validation of the construct sequences, the mini-genes were inserted under control of the CMV promoter into the pCR3 vector and transfected into Huh-7 cells. .. The amplicon sizes of RT-PCR products were measured by a 2100 BioAnalyzer (Agilent Technologies, Santa Clara, CA).

Software:

Article Title: Identification of deleterious rare variants in MTTP, PNPLA3, and TM6SF2 in Japanese males and association studies with NAFLD
Article Snippet: The stained cells were visualized under a fluorescence microscope, and the ratios of GFP-PNPLA3 on lipid droplets to total GFP fluorescence and LipidTOX fluorescence in GFP-expressing cells were measured from 10 randomly selected fields using the associated software (KEYENCE Japan, Osaka, Japan). .. The amplicon sizes of RT-PCR products were measured by a 2100 BioAnalyzer (Agilent Technologies, Santa Clara, CA).

Article Title: A highly effective and versatile technology for the isolation of RNAs from grapevines and other woody perennials for use in virus diagnostics
Article Snippet: The integrity and size distribution of purified RNAs were also evaluated with a 2100 Bioanalyzer (Agilent Technologies, Inc. Santa Clara, CA) equipped with an RNA Nano chip. .. Small RNAs and miRNAs from the total RNA samples isolated from young grapevine leaves with the five commercial kits were analyzed by the Agilent Bioanalyzer equipped with Small RNA Analysis kit (Agilent Technologies, Inc.) and the yields of small RNAs and miRNAs were calculated using the software Bio sizing version B.02.08.

Article Title: VR09 Cell Line: An EBV-Positive Lymphoblastoid Cell Line with In Vivo Characteristics of Diffuse Large B Cell Lymphoma of Activated B-Cell Type
Article Snippet: PCR products were analyzed on a 2100 Bioanalyzer (Agilent Technologies, Waldbronn, Germany), using the Agilent DNA1000 chip and reagents. .. Electronic data were analyzed using the manufacturer’s software (Agilent 2100 Expert).

Article Title: The Effect of Formaldehyde Fixation on RNA
Article Snippet: The compositions of the total HeLa RNA and RNA 50mer preparations were characterized by capillary electrophoresis using a 2100 bioanalyzer (Agilent Technologies, Santa Clara, CA). .. RNA size and concentration were calculated relative to the total RNA nano kit's ladder using computer software (Agilent 2100 Expert Software).

Functional Assay:

Article Title: Identification of deleterious rare variants in MTTP, PNPLA3, and TM6SF2 in Japanese males and association studies with NAFLD
Article Snippet: Paragraph title: Functional evaluation of the rare variants observed in PNPLA3 ... The amplicon sizes of RT-PCR products were measured by a 2100 BioAnalyzer (Agilent Technologies, Santa Clara, CA).

Sample Prep:

Article Title: High quality RNA extraction from Maqui berry for its application in next-generation sequencing
Article Snippet: Three Maqui berry libraries (green fruit, red fruit and blue fruit) were prepared using the TruSeq™ RNA Sample Preparation Kits v2 (Illumina® ). .. The quality of these libraries was assessed with a DNA 1000 kit in a 2100 Bioanalyzer (Agilent Technologies).

In Vitro:

Article Title: VR09 Cell Line: An EBV-Positive Lymphoblastoid Cell Line with In Vivo Characteristics of Diffuse Large B Cell Lymphoma of Activated B-Cell Type
Article Snippet: EBV Status Evaluation The presence of EBV was assessed on both cells cultured in vitro and cells obtained from the tumoral mass developing after in vivo injection of VR09 cell line. .. PCR products were analyzed on a 2100 Bioanalyzer (Agilent Technologies, Waldbronn, Germany), using the Agilent DNA1000 chip and reagents.

Next-Generation Sequencing:

Article Title: High quality RNA extraction from Maqui berry for its application in next-generation sequencing
Article Snippet: Transcriptomics using next-generation sequencing requires high concentration and quality as clean RNA material. .. The quality of these libraries was assessed with a DNA 1000 kit in a 2100 Bioanalyzer (Agilent Technologies).

Spectrophotometry:

Article Title: RNase L Interacts with Filamin A To Regulate Actin Dynamics and Barrier Function for Viral Entry
Article Snippet: At the indicated times, the total RNA was isolated from transfected cells using Trizol reagent (Invitrogen/Life Technologies) and quantitated by measuring absorbance at 260 nm using a NanoDrop spectrophotometer (Thermo Scientific, Wilmington, DE). .. RNAs (2 µg) were separated on RNA chips and analyzed with a 2100 Bioanalyzer (Agilent Technologies) to monitor characteristic rRNA cleavage products generated by RNase L activity as described previously ( ).

Article Title: A highly effective and versatile technology for the isolation of RNAs from grapevines and other woody perennials for use in virus diagnostics
Article Snippet: Assessment of yield and quality of total RNA and small RNA The purity and concentration of the total RNA preparations were assessed with a NanoDrop spectrophotometer (ND-1000, Thermo Scientific, Delaware, USA) at the wavelengths of 230, 260, and 280 nm. .. The integrity and size distribution of purified RNAs were also evaluated with a 2100 Bioanalyzer (Agilent Technologies, Inc. Santa Clara, CA) equipped with an RNA Nano chip.

Concentration Assay:

Article Title: Effect of lesion proximity on the regenerative response of long descending propriospinal neurons after spinal transection injury
Article Snippet: Total RNA concentration was determined by the RNA 6000 Pico RNA Assay (Agilent Technologies; Santa Clara, CA). .. Quality of the RNA extraction was determined utilizing a 2100 bioanalyzer (Agilent Technologies; Santa Clara, CA) which provided an RNA Integrity Number (RIN), and corresponding pseudo gel (Fig. ).

Article Title: High quality RNA extraction from Maqui berry for its application in next-generation sequencing
Article Snippet: Transcriptomics using next-generation sequencing requires high concentration and quality as clean RNA material. .. The quality of these libraries was assessed with a DNA 1000 kit in a 2100 Bioanalyzer (Agilent Technologies).

Article Title: The characterization of four gene expression analysis in circulating tumor cells made by Multiplex-PCR from the AdnaTest kit on the lab-on-a-chip Agilent DNA 1000 platform
Article Snippet: .. Subjects The study describes the PCR fragment size and concentration determination performed with the commercially available Agilent DNA 1000 reagent kit (Agilent Technologies, USA, Santa Clara) on the 2100 Bioanalyzer (Agilent Technologies, USA, Santa Clara). ..

Article Title: Effects of Acute Aerobic Exercise on Rats Serum Extracellular Vesicles Diameter, Concentration and Small RNAs Content
Article Snippet: .. The RNA was eluted from the column after washing it twice with 50 μl of RNase-free water, incubation for 1 min at room temperature and centrifuging for 1 min at 11,000 × g. The 2100 Bioanalyzer (Agilent) small RNA chip was used to analyze concentration and size from the purified small RNA samples. ..

Article Title: A highly effective and versatile technology for the isolation of RNAs from grapevines and other woody perennials for use in virus diagnostics
Article Snippet: Assessment of yield and quality of total RNA and small RNA The purity and concentration of the total RNA preparations were assessed with a NanoDrop spectrophotometer (ND-1000, Thermo Scientific, Delaware, USA) at the wavelengths of 230, 260, and 280 nm. .. The integrity and size distribution of purified RNAs were also evaluated with a 2100 Bioanalyzer (Agilent Technologies, Inc. Santa Clara, CA) equipped with an RNA Nano chip.

Article Title: Comprehensive Investigation of miRNome Identifies Novel Candidate miRNA-mRNA Interactions Implicated in T-Cell Acute Lymphoblastic Leukemia
Article Snippet: RNA concentration was measured with Quantus Fluorometer (Promega) using Qubit HS RNA Assay Kit (Thermo Fisher Scientific). .. RNA integrity was determined with 4200 Tapestation using High Sensitivity RNA ScreenTape (Agilent Technologies) and 2100 Bioanalyzer using RNA 6000 Nano Assay (Agilent Technologies).

Article Title: The Effect of Formaldehyde Fixation on RNA
Article Snippet: The compositions of the total HeLa RNA and RNA 50mer preparations were characterized by capillary electrophoresis using a 2100 bioanalyzer (Agilent Technologies, Santa Clara, CA). .. RNA size and concentration were calculated relative to the total RNA nano kit's ladder using computer software (Agilent 2100 Expert Software).

Staining:

Article Title: Identification of deleterious rare variants in MTTP, PNPLA3, and TM6SF2 in Japanese males and association studies with NAFLD
Article Snippet: The stained cells were visualized under a fluorescence microscope, and the ratios of GFP-PNPLA3 on lipid droplets to total GFP fluorescence and LipidTOX fluorescence in GFP-expressing cells were measured from 10 randomly selected fields using the associated software (KEYENCE Japan, Osaka, Japan). .. The amplicon sizes of RT-PCR products were measured by a 2100 BioAnalyzer (Agilent Technologies, Santa Clara, CA).

Article Title: A highly effective and versatile technology for the isolation of RNAs from grapevines and other woody perennials for use in virus diagnostics
Article Snippet: RNA integrity was verified based on the 28S and 18S rRNA bands after electrophoresis on 1.5 % formaldehyde–agarose gel, stained with ethidium bromide and visualization with UV light. .. The integrity and size distribution of purified RNAs were also evaluated with a 2100 Bioanalyzer (Agilent Technologies, Inc. Santa Clara, CA) equipped with an RNA Nano chip.

Article Title: The Effect of Formaldehyde Fixation on RNA
Article Snippet: The compositions of the total HeLa RNA and RNA 50mer preparations were characterized by capillary electrophoresis using a 2100 bioanalyzer (Agilent Technologies, Santa Clara, CA). .. Some experiments used 1.5% 4-morpholinepropane sulfonic acid–formaldehyde denaturing agarose gels stained with ethidium bromide and photographed, as described.

Variant Assay:

Article Title: Identification of deleterious rare variants in MTTP, PNPLA3, and TM6SF2 in Japanese males and association studies with NAFLD
Article Snippet: The PCR fragment of introns 6–8 of TM6SF2 from the c.712-2A > T mutation carrier and introns 4–5 of PNPLA3 from the rs148469440 variant carrier were inserted into intron 1 of ACKR1 and TRIB2 , respectively. .. The amplicon sizes of RT-PCR products were measured by a 2100 BioAnalyzer (Agilent Technologies, Santa Clara, CA).

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    Agilent technologies 2100 bioanalyzer
    Postfixation processing of an RNA 50mer with excess formaldehyde removed. Gel-simulated image (Agilent <t>2100</t> <t>Bioanalyzer)</t> of the RNA 50mer fixed in 5% buffered formaldehyde (pH 7.4) for 2 hours. A: Lane L: RNA ladder. Lane 1: Non–formaldehyde-treated
    2100 Bioanalyzer, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 148 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/2100 bioanalyzer/product/Agilent technologies
    Average 99 stars, based on 148 article reviews
    Price from $9.99 to $1999.99
    2100 bioanalyzer - by Bioz Stars, 2020-01
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    99
    Agilent technologies dna high sensitivity kit
    Analysis of plasma <t>cfDNA</t> obtained from one representative pregnant donor blood stored at 22°C using Agilent Bioanalyzer 2100 instrument and Agilent <t>DNA</t> high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in K 3 EDTA tubes at days 0, 3, 7, 14 and 28. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in ProTeck tubes at days 0, 3, 7, 14 and 28. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.
    Dna High Sensitivity Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna high sensitivity kit/product/Agilent technologies
    Average 99 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    dna high sensitivity kit - by Bioz Stars, 2020-01
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    99
    Agilent technologies bioanalyzer 2100 instrument
    Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 22°C using Agilent <t>Bioanalyzer</t> 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in K 3 EDTA tubes at days 0, 3, 7, 14 and 28. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in ProTeck tubes at days 0, 3, 7, 14 and 28. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.
    Bioanalyzer 2100 Instrument, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 90 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bioanalyzer 2100 instrument/product/Agilent technologies
    Average 99 stars, based on 90 article reviews
    Price from $9.99 to $1999.99
    bioanalyzer 2100 instrument - by Bioz Stars, 2020-01
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    Image Search Results


    Postfixation processing of an RNA 50mer with excess formaldehyde removed. Gel-simulated image (Agilent 2100 Bioanalyzer) of the RNA 50mer fixed in 5% buffered formaldehyde (pH 7.4) for 2 hours. A: Lane L: RNA ladder. Lane 1: Non–formaldehyde-treated

    Journal: The Journal of Molecular Diagnostics : JMD

    Article Title: The Effect of Formaldehyde Fixation on RNA

    doi: 10.1016/j.jmoldx.2011.01.010

    Figure Lengend Snippet: Postfixation processing of an RNA 50mer with excess formaldehyde removed. Gel-simulated image (Agilent 2100 Bioanalyzer) of the RNA 50mer fixed in 5% buffered formaldehyde (pH 7.4) for 2 hours. A: Lane L: RNA ladder. Lane 1: Non–formaldehyde-treated

    Article Snippet: The compositions of the total HeLa RNA and RNA 50mer preparations were characterized by capillary electrophoresis using a 2100 bioanalyzer (Agilent Technologies, Santa Clara, CA).

    Techniques:

    Effects of fixation and postfixation processing on total cellular RNA: gel-simulated image (Agilent 2100 Bioanalyzer) of total cellular RNA fixed in 5% buffered formaldehyde (pH 7.4) for 2 hours. Lane L: RNA ladder. Lane 1: Native non–formaldehyde-treated

    Journal: The Journal of Molecular Diagnostics : JMD

    Article Title: The Effect of Formaldehyde Fixation on RNA

    doi: 10.1016/j.jmoldx.2011.01.010

    Figure Lengend Snippet: Effects of fixation and postfixation processing on total cellular RNA: gel-simulated image (Agilent 2100 Bioanalyzer) of total cellular RNA fixed in 5% buffered formaldehyde (pH 7.4) for 2 hours. Lane L: RNA ladder. Lane 1: Native non–formaldehyde-treated

    Article Snippet: The compositions of the total HeLa RNA and RNA 50mer preparations were characterized by capillary electrophoresis using a 2100 bioanalyzer (Agilent Technologies, Santa Clara, CA).

    Techniques:

    Effects of buffer composition on postfixation processing of an RNA 50mer: gel-simulated image (Agilent 2100 Bioanalyzer). Lane L: RNA ladder. Lane 1: Native RNA 50mer. Lane 2: RNA 50mer fixed in 5% buffered formaldehyde (pH 7.4) for 2 hours. Excess formaldehyde

    Journal: The Journal of Molecular Diagnostics : JMD

    Article Title: The Effect of Formaldehyde Fixation on RNA

    doi: 10.1016/j.jmoldx.2011.01.010

    Figure Lengend Snippet: Effects of buffer composition on postfixation processing of an RNA 50mer: gel-simulated image (Agilent 2100 Bioanalyzer). Lane L: RNA ladder. Lane 1: Native RNA 50mer. Lane 2: RNA 50mer fixed in 5% buffered formaldehyde (pH 7.4) for 2 hours. Excess formaldehyde

    Article Snippet: The compositions of the total HeLa RNA and RNA 50mer preparations were characterized by capillary electrophoresis using a 2100 bioanalyzer (Agilent Technologies, Santa Clara, CA).

    Techniques:

    Postformaldehyde fixation processing of an RNA 50mer. Gel-simulated image (Agilent 2100 Bioanalyzer) of the RNA 50mer fixed in 5% buffered formaldehyde (pH 7.4) for 2 hours and then heated at 70°C for 30 minutes, where indicated, in the following

    Journal: The Journal of Molecular Diagnostics : JMD

    Article Title: The Effect of Formaldehyde Fixation on RNA

    doi: 10.1016/j.jmoldx.2011.01.010

    Figure Lengend Snippet: Postformaldehyde fixation processing of an RNA 50mer. Gel-simulated image (Agilent 2100 Bioanalyzer) of the RNA 50mer fixed in 5% buffered formaldehyde (pH 7.4) for 2 hours and then heated at 70°C for 30 minutes, where indicated, in the following

    Article Snippet: The compositions of the total HeLa RNA and RNA 50mer preparations were characterized by capillary electrophoresis using a 2100 bioanalyzer (Agilent Technologies, Santa Clara, CA).

    Techniques:

    Effects of fixation and postfixation processing on total HeLa RNA. Electropherograms (Agilent 2100 Bioanalyzer) of total cellular RNA fixed in 5% buffered formaldehyde (pH 7.4) for 2 hours. FU denotes fluorescence units. A: Non–formaldehyde-treated

    Journal: The Journal of Molecular Diagnostics : JMD

    Article Title: The Effect of Formaldehyde Fixation on RNA

    doi: 10.1016/j.jmoldx.2011.01.010

    Figure Lengend Snippet: Effects of fixation and postfixation processing on total HeLa RNA. Electropherograms (Agilent 2100 Bioanalyzer) of total cellular RNA fixed in 5% buffered formaldehyde (pH 7.4) for 2 hours. FU denotes fluorescence units. A: Non–formaldehyde-treated

    Article Snippet: The compositions of the total HeLa RNA and RNA 50mer preparations were characterized by capillary electrophoresis using a 2100 bioanalyzer (Agilent Technologies, Santa Clara, CA).

    Techniques: Fluorescence

    Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 22°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in K 3 EDTA tubes at days 0, 3, 7, 14 and 28. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in ProTeck tubes at days 0, 3, 7, 14 and 28. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Journal: PLoS ONE

    Article Title: A novel approach to stabilize fetal cell-free DNA fraction in maternal blood samples for extended period of time

    doi: 10.1371/journal.pone.0208508

    Figure Lengend Snippet: Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 22°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in K 3 EDTA tubes at days 0, 3, 7, 14 and 28. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in ProTeck tubes at days 0, 3, 7, 14 and 28. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Article Snippet: Concentrated maternal cfDNA was analyzed by Agilent Bioanalyzer 2100 instrument and Agilent DNA High Sensitivity Kit following manufacturer’s recommended protocol.

    Techniques:

    Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 30°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored in K 3 EDTA tubes at days 0, 2, 3, 7 and 14. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored in ProTeck tubes at days 0, 2, 3, 7 and 14. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Journal: PLoS ONE

    Article Title: A novel approach to stabilize fetal cell-free DNA fraction in maternal blood samples for extended period of time

    doi: 10.1371/journal.pone.0208508

    Figure Lengend Snippet: Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 30°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored in K 3 EDTA tubes at days 0, 2, 3, 7 and 14. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored in ProTeck tubes at days 0, 2, 3, 7 and 14. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Article Snippet: Concentrated maternal cfDNA was analyzed by Agilent Bioanalyzer 2100 instrument and Agilent DNA High Sensitivity Kit following manufacturer’s recommended protocol.

    Techniques:

    Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 4°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored in K 3 EDTA tubes at days 0, 3, 7, 14 and 21. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored in ProTeck tubes at days 0, 3, 7, 14 and 21. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Journal: PLoS ONE

    Article Title: A novel approach to stabilize fetal cell-free DNA fraction in maternal blood samples for extended period of time

    doi: 10.1371/journal.pone.0208508

    Figure Lengend Snippet: Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 4°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored in K 3 EDTA tubes at days 0, 3, 7, 14 and 21. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored in ProTeck tubes at days 0, 3, 7, 14 and 21. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Article Snippet: Concentrated maternal cfDNA was analyzed by Agilent Bioanalyzer 2100 instrument and Agilent DNA High Sensitivity Kit following manufacturer’s recommended protocol.

    Techniques:

    Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 22°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in K 3 EDTA tubes at days 0, 3, 7, 14 and 28. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in ProTeck tubes at days 0, 3, 7, 14 and 28. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Journal: PLoS ONE

    Article Title: A novel approach to stabilize fetal cell-free DNA fraction in maternal blood samples for extended period of time

    doi: 10.1371/journal.pone.0208508

    Figure Lengend Snippet: Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 22°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in K 3 EDTA tubes at days 0, 3, 7, 14 and 28. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in ProTeck tubes at days 0, 3, 7, 14 and 28. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Article Snippet: Concentrated maternal cfDNA was analyzed by Agilent Bioanalyzer 2100 instrument and Agilent DNA High Sensitivity Kit following manufacturer’s recommended protocol.

    Techniques:

    Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 30°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored in K 3 EDTA tubes at days 0, 2, 3, 7 and 14. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored in ProTeck tubes at days 0, 2, 3, 7 and 14. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Journal: PLoS ONE

    Article Title: A novel approach to stabilize fetal cell-free DNA fraction in maternal blood samples for extended period of time

    doi: 10.1371/journal.pone.0208508

    Figure Lengend Snippet: Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 30°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored in K 3 EDTA tubes at days 0, 2, 3, 7 and 14. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored in ProTeck tubes at days 0, 2, 3, 7 and 14. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Article Snippet: Concentrated maternal cfDNA was analyzed by Agilent Bioanalyzer 2100 instrument and Agilent DNA High Sensitivity Kit following manufacturer’s recommended protocol.

    Techniques:

    Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 4°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored in K 3 EDTA tubes at days 0, 3, 7, 14 and 21. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored in ProTeck tubes at days 0, 3, 7, 14 and 21. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Journal: PLoS ONE

    Article Title: A novel approach to stabilize fetal cell-free DNA fraction in maternal blood samples for extended period of time

    doi: 10.1371/journal.pone.0208508

    Figure Lengend Snippet: Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 4°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored in K 3 EDTA tubes at days 0, 3, 7, 14 and 21. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored in ProTeck tubes at days 0, 3, 7, 14 and 21. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Article Snippet: Concentrated maternal cfDNA was analyzed by Agilent Bioanalyzer 2100 instrument and Agilent DNA High Sensitivity Kit following manufacturer’s recommended protocol.

    Techniques:

    Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 22°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in K 3 EDTA tubes at days 0, 3, 7, 14 and 28. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in ProTeck tubes at days 0, 3, 7, 14 and 28. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Journal: PLoS ONE

    Article Title: A novel approach to stabilize fetal cell-free DNA fraction in maternal blood samples for extended period of time

    doi: 10.1371/journal.pone.0208508

    Figure Lengend Snippet: Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 22°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in K 3 EDTA tubes at days 0, 3, 7, 14 and 28. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in ProTeck tubes at days 0, 3, 7, 14 and 28. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Article Snippet: Concentrated maternal cfDNA was analyzed by Agilent Bioanalyzer 2100 instrument and Agilent DNA High Sensitivity Kit following manufacturer’s recommended protocol.

    Techniques:

    Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 30°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored in K 3 EDTA tubes at days 0, 2, 3, 7 and 14. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored in ProTeck tubes at days 0, 2, 3, 7 and 14. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Journal: PLoS ONE

    Article Title: A novel approach to stabilize fetal cell-free DNA fraction in maternal blood samples for extended period of time

    doi: 10.1371/journal.pone.0208508

    Figure Lengend Snippet: Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 30°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored in K 3 EDTA tubes at days 0, 2, 3, 7 and 14. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored in ProTeck tubes at days 0, 2, 3, 7 and 14. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Article Snippet: Concentrated maternal cfDNA was analyzed by Agilent Bioanalyzer 2100 instrument and Agilent DNA High Sensitivity Kit following manufacturer’s recommended protocol.

    Techniques:

    Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 4°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored in K 3 EDTA tubes at days 0, 3, 7, 14 and 21. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored in ProTeck tubes at days 0, 3, 7, 14 and 21. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Journal: PLoS ONE

    Article Title: A novel approach to stabilize fetal cell-free DNA fraction in maternal blood samples for extended period of time

    doi: 10.1371/journal.pone.0208508

    Figure Lengend Snippet: Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 4°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored in K 3 EDTA tubes at days 0, 3, 7, 14 and 21. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored in ProTeck tubes at days 0, 3, 7, 14 and 21. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Article Snippet: Concentrated maternal cfDNA was analyzed by Agilent Bioanalyzer 2100 instrument and Agilent DNA High Sensitivity Kit following manufacturer’s recommended protocol.

    Techniques: