2100 bioanalyser rna 6000 nano labchip kit agilent technologies santa clara usa  (Agilent technologies)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    Agilent technologies 2100 bioanalyser rna 6000 nano labchip kit agilent technologies santa clara usa
    2100 Bioanalyser Rna 6000 Nano Labchip Kit Agilent Technologies Santa Clara Usa, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/2100 bioanalyser rna 6000 nano labchip kit agilent technologies santa clara usa/product/Agilent technologies
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    2100 bioanalyser rna 6000 nano labchip kit agilent technologies santa clara usa - by Bioz Stars, 2019-12
    99/100 stars

    Images

    Related Articles

    Centrifugation:

    Article Title: Sublethal Concentrations of Antibiotics Cause Shift to Anaerobic Metabolism in Listeria monocytogenes and Induce Phenotypes Linked to Antibiotic Tolerance
    Article Snippet: Cells were pelleted by centrifugation and stored at -80°C until total RNA extraction using Trizol (Invitrogen) according to . .. Total RNA quality and quantity were assessed by an Agilent 2100 Bioanalyser using an RNA 6000 Nano chip and Nanodrop spectrophotometer, respectively.

    Article Title: Transcriptomic profiling of host-parasite interactions in the microsporidian Trachipleistophora hominis
    Article Snippet: Cells suspended in RNAprotect (Qiagen) were thawed and pelleted by centrifugation at 400 g. Total RNA was purified from each sample using the standard TRIzol (Invitrogen) extraction protocol, with the addition of a bead beating step (three times for 20 s at 5 m/s, with 10 mins incubation on ice between beating). .. The RNA integrity and concentration was assessed using the Agilent RNA 6000 Nano Kit on the Agilent 2100 BioAnalyser.

    Article Title: Generation of novel pharmacogenomic candidates in the response to methotrexate in juvenile idiopathic arthritis: correlation between gene expression and genotype
    Article Snippet: PBMC were prepared from venous blood by standard density centrifugation. .. High quality and purity of RNA were confirmed using the Bioanalyser 2100 (Agilent Technologies, Palo Alto, CA). cDNA and subsequent cRNA synthesis was performed as described.

    Amplification:

    Article Title: Identification of ovule transcripts from the Apospory-Specific Genomic Region (ASGR)-carrier chromosome
    Article Snippet: Paragraph title: RNA amplification and ds-cDNA synthesis for Roche 454 sequencing ... Size-range and quantity of ds-cDNA were also analyzed by both gel electrophoresis and using the Agilent 2100 Bioanalyser before submitting the samples for sequencing.

    Article Title: Accurate diagnosis of mismatch repair deficiency in colorectal cancer using high-quality DNA samples from cultured stem cells
    Article Snippet: Three electrophoretic runs were performed for each sample (BAT25 /D2S123, D5S346 /D17S250 , and BAT26 ) according to the standard protocol [ ] ( ). .. Amplified DNA fragments were purified with QIAquick PCR Purification Kit (Qiagen), applied on the DNA LabChip of the Agilent DNA 1000 Kit (Agilent Technologies, Santa Clara, CA, USA), and analyzed with an Agilent 2100 Bioanalyser instrument (Agilent Technologies) according to the previous protocol [ ]. .. The electropherogram of each cancer spheroid line was overlaid with that of the normal epithelial spheroid line derived from the same patient.

    Article Title: Full-length transcriptome of Misgurnus anguillicaudatus provides insights into evolution of genus Misgurnus
    Article Snippet: RNA quality and concentration were determined using NanoDrop 2000 (Thermo Scientific, DE, USA) and Agilent Bioanalyser 2100 (Agilent Technologies, CA, USA), respectively. .. The first-strand cDNA was synthesized using SMARTer PCR cDNA Synthesis Kit (Clontech, CA, USA).

    DNA Synthesis:

    Article Title: Expression of Genes for Drug Transporters in the Human Female Genital Tract and Modulatory Effect of Antiretroviral Drugs
    Article Snippet: Integrity and quantification of RNA were evaluated using the Agilent RNA 6000 Nano Kit (Agilent Technologies, Milan, Italy) on the Agilent 2100 Bioanalyser (Agilent Technologies). .. Integrity and quantification of RNA were evaluated using the Agilent RNA 6000 Nano Kit (Agilent Technologies, Milan, Italy) on the Agilent 2100 Bioanalyser (Agilent Technologies).

    Mass Spectrometry:

    Article Title: Full-length transcriptome of Misgurnus anguillicaudatus provides insights into evolution of genus Misgurnus
    Article Snippet: After anaesthetizing with MS-222 (100 mg/L), 12 tissues (i.e. blood, gill, skin, muscle, liver, spleen, intestine, ovary, testis, kidney, heart and brain) of a male and a female individual were immediately sampled separately. .. RNA quality and concentration were determined using NanoDrop 2000 (Thermo Scientific, DE, USA) and Agilent Bioanalyser 2100 (Agilent Technologies, CA, USA), respectively.

    Synthesized:

    Article Title: Heart function and thoracic aorta gene expression profiling studies of ginseng combined with different herbal medicines in eNOS knockout mice
    Article Snippet: Total RNA was extracted using mirVana RNA Isolation Kit (Thermo Scientific, Waltham, USA), and quantified using the NanoDrop ND-2000 (Thermo Scientific, Waltham, USA) and the RNA integrity was evaluated using Agilent Bioanalyser 2100 (Agilent Technologies, Palo Alto, USA). .. The Agilent SurePrint G3 Mouse Gene Expression (8*60 K, Design ID: 028005) was used in this experiment.

    Article Title: Full-length transcriptome of Misgurnus anguillicaudatus provides insights into evolution of genus Misgurnus
    Article Snippet: RNA quality and concentration were determined using NanoDrop 2000 (Thermo Scientific, DE, USA) and Agilent Bioanalyser 2100 (Agilent Technologies, CA, USA), respectively. .. RNA quality and concentration were determined using NanoDrop 2000 (Thermo Scientific, DE, USA) and Agilent Bioanalyser 2100 (Agilent Technologies, CA, USA), respectively.

    Quantitative RT-PCR:

    Article Title: Molecular and proteomic analyses highlight the importance of ubiquitination for the stress resistance, metabolic adaptation, morphogenetic regulation and virulence of Candida albicans
    Article Snippet: UBI4 mRNA levels were measured by qRT-PCR. .. RNA was extracted with Trizol (GibcoBRL, Grand Island, NY, USA) as described previously , and RNA integrity assayed on an Agilent Bioanalyser 2100 (Stockport, UK).

    Real-time Polymerase Chain Reaction:

    Article Title: The Anti-Proliferative Effects of Enterolactone in Prostate Cancer Cells: Evidence for the Role of DNA Licencing Genes, mi-R106b Cluster Expression, and PTEN Dosage
    Article Snippet: For each gene and time point, two biological replicates (with triplicate qPCR measurements) of 5 × 105 cells were used. .. RNA quantity and integrity was determined based on A260:280 and A260:230 nm ratios using a NanoDrop 1000 spectrophotometer (Thermo Fisher Scientific, Melbourne, Australia) and a Agilent 2100 bioanalyser (Agilent, Santa Clara, CA, USA).

    Article Title: Expression of Genes for Drug Transporters in the Human Female Genital Tract and Modulatory Effect of Antiretroviral Drugs
    Article Snippet: Paragraph title: Purification of total RNA, cDNA synthesis and quantitative PCR ... Integrity and quantification of RNA were evaluated using the Agilent RNA 6000 Nano Kit (Agilent Technologies, Milan, Italy) on the Agilent 2100 Bioanalyser (Agilent Technologies).

    Article Title: Molecular and proteomic analyses highlight the importance of ubiquitination for the stress resistance, metabolic adaptation, morphogenetic regulation and virulence of Candida albicans
    Article Snippet: RNA was extracted with Trizol (GibcoBRL, Grand Island, NY, USA) as described previously , and RNA integrity assayed on an Agilent Bioanalyser 2100 (Stockport, UK). .. For qRT-PCR, samples were incubated at room temperature for 15 min in a 20 µl reaction mix containing 2 µg RNA, 2 µl DNase I buffer (Invitrogen; Paisley, UK), 1.5 µl DNase I and 1.5 µl RNase OUT (Invitrogen). cDNA was prepared using Superscript II (Invitrogen) as per the manufacturer's protocol.

    Article Title: Identification of superior reference genes for data normalisation of expression studies via quantitative PCR in hybrid roses (Rosa hybrida)
    Article Snippet: The RNA quality was assessed by gel electrophoresis and for a subset of samples using a Bioanalyser 2100 lab chip (Agilent, Santa Clara). .. The RNA quality was assessed by gel electrophoresis and for a subset of samples using a Bioanalyser 2100 lab chip (Agilent, Santa Clara).

    Microarray:

    Article Title: HMA4 expression in tobacco reduces Cd accumulation due to the induction of the apoplastic barrier
    Article Snippet: All RNA samples were quantified at A 260 using a Nanodrop spectrophotometer ND100 (Nanodrop, Wilimington, DE, USA). .. The quality of RNA used for microarray analysis was checked using an Agilent 2100 Bioanalyser (Agilent Technologies, Santa Clara, CA, USA), according to the manufacturer’s instructions. .. Microarray-based analysis was performed to compare root gene expression profiles in tobacco expressing 35S-AtHMA4 (line no. 9) and wild-type plants, which were grown in the presence of 0.25 μM Cd.

    Article Title: Sialic Acid Utilisation and Synthesis in the Neonatal Rat Revisited
    Article Snippet: Paragraph title: Microarray Analysis ... After extraction, RNA was quantified with the RiboGreen RNA Quantification Kit (Molecular Probes) and monitored for quality on a Agilent 2100 Bioanalyser (RNA integrity numbers ≧8 for high quality).

    Article Title: Nutrigenomic and Nutritional Analyses Reveal the Effects of Pelleted Feeds on Asian Seabass (Lates calcarifer)
    Article Snippet: Paragraph title: 2.8 Microarray hybridization and analysis ... The RNA integrity number (RIN) of extracted total RNA was determined by using Agilent 2100 Bioanalyser (Agilent Technologies, Nærum, Denmark).

    Article Title: Heart function and thoracic aorta gene expression profiling studies of ginseng combined with different herbal medicines in eNOS knockout mice
    Article Snippet: Paragraph title: Microarray Analysis ... Total RNA was extracted using mirVana RNA Isolation Kit (Thermo Scientific, Waltham, USA), and quantified using the NanoDrop ND-2000 (Thermo Scientific, Waltham, USA) and the RNA integrity was evaluated using Agilent Bioanalyser 2100 (Agilent Technologies, Palo Alto, USA).

    Article Title: The Intensity of IUGR-Induced Transcriptome Deregulations Is Inversely Correlated with the Onset of Organ Function in a Rat Model
    Article Snippet: Paragraph title: RNA preparation, cDNA synthesis, and microarray hybridizations ... RNA isolation was performed according to standard protocols using Trizol reagent and controlled (precise integrity checks and sample quantitation) by Agilent bioanalyser 2100.

    Incubation:

    Article Title: Transcriptomic profiling of host-parasite interactions in the microsporidian Trachipleistophora hominis
    Article Snippet: Cells suspended in RNAprotect (Qiagen) were thawed and pelleted by centrifugation at 400 g. Total RNA was purified from each sample using the standard TRIzol (Invitrogen) extraction protocol, with the addition of a bead beating step (three times for 20 s at 5 m/s, with 10 mins incubation on ice between beating). .. The RNA integrity and concentration was assessed using the Agilent RNA 6000 Nano Kit on the Agilent 2100 BioAnalyser.

    Expressing:

    Article Title: A Splice Mutation in the PHKG1 Gene Causes High Glycogen Content and Low Meat Quality in Pig Skeletal Muscle
    Article Snippet: RNA quantity and integrity were assessed using a NanoDrop ND-1000 Spectrophotometer (Thermo Fisher Scientific) and a 2100 Bioanalyser (Agilent). .. For mapping clean tags to reference transcript sets or to the pig reference genome (Sus Scrofa Build 10.2) , we created virtual libraries containing all the possible 17-base length sequences of these resources located next to an Nla III restriction site.

    Article Title: The Anti-Proliferative Effects of Enterolactone in Prostate Cancer Cells: Evidence for the Role of DNA Licencing Genes, mi-R106b Cluster Expression, and PTEN Dosage
    Article Snippet: Paragraph title: 2.5. Quantification of Gene Expression—mRNA and miRNA Genes ... RNA quantity and integrity was determined based on A260:280 and A260:230 nm ratios using a NanoDrop 1000 spectrophotometer (Thermo Fisher Scientific, Melbourne, Australia) and a Agilent 2100 bioanalyser (Agilent, Santa Clara, CA, USA).

    Article Title: Sialic Acid Utilisation and Synthesis in the Neonatal Rat Revisited
    Article Snippet: After extraction, RNA was quantified with the RiboGreen RNA Quantification Kit (Molecular Probes) and monitored for quality on a Agilent 2100 Bioanalyser (RNA integrity numbers ≧8 for high quality). .. 750 ng biotin-labelled cRNA was added to the hybridization mix, which contained control oligonucleotides (such as negative and hybridization controls), hybridization buffer, and water.

    Article Title: Nutrigenomic and Nutritional Analyses Reveal the Effects of Pelleted Feeds on Asian Seabass (Lates calcarifer)
    Article Snippet: The RNA integrity number (RIN) of extracted total RNA was determined by using Agilent 2100 Bioanalyser (Agilent Technologies, Nærum, Denmark). .. Microarray-based transcriptomics were carried out using Agilent SurePrint G3 custom gene expression microarrays (8X60K; Cat No. G4102A) with probes covering an estimated ~70% of the seabass transcriptome (estimated based on BLASTX-search against the Nile tilapia RefSeq protein dataset).

    Article Title: Heart function and thoracic aorta gene expression profiling studies of ginseng combined with different herbal medicines in eNOS knockout mice
    Article Snippet: Total RNA was extracted using mirVana RNA Isolation Kit (Thermo Scientific, Waltham, USA), and quantified using the NanoDrop ND-2000 (Thermo Scientific, Waltham, USA) and the RNA integrity was evaluated using Agilent Bioanalyser 2100 (Agilent Technologies, Palo Alto, USA). .. Sample labelling, microarray hybridization and washing were successively performed according to the manufacturer’s instructions.

    Article Title: Generation of novel pharmacogenomic candidates in the response to methotrexate in juvenile idiopathic arthritis: correlation between gene expression and genotype
    Article Snippet: Paragraph title: Gene expression profiling ... High quality and purity of RNA were confirmed using the Bioanalyser 2100 (Agilent Technologies, Palo Alto, CA). cDNA and subsequent cRNA synthesis was performed as described.

    Transformation Assay:

    Article Title: Nutrigenomic and Nutritional Analyses Reveal the Effects of Pelleted Feeds on Asian Seabass (Lates calcarifer)
    Article Snippet: The RNA integrity number (RIN) of extracted total RNA was determined by using Agilent 2100 Bioanalyser (Agilent Technologies, Nærum, Denmark). .. The RNA integrity number (RIN) of extracted total RNA was determined by using Agilent 2100 Bioanalyser (Agilent Technologies, Nærum, Denmark).

    Hybridization:

    Article Title: Sialic Acid Utilisation and Synthesis in the Neonatal Rat Revisited
    Article Snippet: After extraction, RNA was quantified with the RiboGreen RNA Quantification Kit (Molecular Probes) and monitored for quality on a Agilent 2100 Bioanalyser (RNA integrity numbers ≧8 for high quality). .. Briefly, 200 ng total RNA was used to produce double-stranded cDNA followed by in vitro transcription and cRNA labelling with biotin.

    Article Title: Nutrigenomic and Nutritional Analyses Reveal the Effects of Pelleted Feeds on Asian Seabass (Lates calcarifer)
    Article Snippet: Paragraph title: 2.8 Microarray hybridization and analysis ... The RNA integrity number (RIN) of extracted total RNA was determined by using Agilent 2100 Bioanalyser (Agilent Technologies, Nærum, Denmark).

    Sequencing:

    Article Title: Identification of ovule transcripts from the Apospory-Specific Genomic Region (ASGR)-carrier chromosome
    Article Snippet: For each sample, an equal amount of amplified mRNA from the three biological replicates was pooled for ds-cDNA synthesis following the protocol developed by the Schnable lab [ ]. .. Size-range and quantity of ds-cDNA were also analyzed by both gel electrophoresis and using the Agilent 2100 Bioanalyser before submitting the samples for sequencing. .. About 6 μg of ds-cDNA from both PS26 and BC8 was submitted to the Genome Sequencing Center at Washington University for 454-FLX sequencing.

    Article Title: Transcriptomic profiling of host-parasite interactions in the microsporidian Trachipleistophora hominis
    Article Snippet: Paragraph title: Preparation of RNA for sequencing ... The RNA integrity and concentration was assessed using the Agilent RNA 6000 Nano Kit on the Agilent 2100 BioAnalyser.

    Article Title: Nanopore-based detection and characterization of yam viruses
    Article Snippet: Paragraph title: Small RNA extraction and Illumina sequencing ... The small RNA fraction was quantified in a Qubit fluorimeter using a Qubit RNA HS Assay Kit (Thermo Fischer Scientific, Waltham, USA) and checked for quality in a bioanalyser 2100 (Agilent).

    Article Title: Full-length transcriptome of Misgurnus anguillicaudatus provides insights into evolution of genus Misgurnus
    Article Snippet: RNA quality and concentration were determined using NanoDrop 2000 (Thermo Scientific, DE, USA) and Agilent Bioanalyser 2100 (Agilent Technologies, CA, USA), respectively. .. The first-strand cDNA was synthesized using SMARTer PCR cDNA Synthesis Kit (Clontech, CA, USA).

    Nucleic Acid Electrophoresis:

    Article Title: Identification of ovule transcripts from the Apospory-Specific Genomic Region (ASGR)-carrier chromosome
    Article Snippet: For each sample, an equal amount of amplified mRNA from the three biological replicates was pooled for ds-cDNA synthesis following the protocol developed by the Schnable lab [ ]. .. Size-range and quantity of ds-cDNA were also analyzed by both gel electrophoresis and using the Agilent 2100 Bioanalyser before submitting the samples for sequencing. .. About 6 μg of ds-cDNA from both PS26 and BC8 was submitted to the Genome Sequencing Center at Washington University for 454-FLX sequencing.

    Article Title: Identification of superior reference genes for data normalisation of expression studies via quantitative PCR in hybrid roses (Rosa hybrida)
    Article Snippet: The RNA concentration was assessed spectrophotometrically at 260 nm and was checked for purity by determining the OD 260 nm/280 nm and the OD 260 nm/230 nm ratios, respectively. .. The RNA quality was assessed by gel electrophoresis and for a subset of samples using a Bioanalyser 2100 lab chip (Agilent, Santa Clara). .. For each sample, 300 ng of total RNA was reverse-transcribed using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Austin) and 1 μg of an oligo-(dT)18 Primer (Fermentas, St. Leon-Rot) according to the manufacturer's instructions.

    RNA Sequencing Assay:

    Article Title: Sublethal Concentrations of Antibiotics Cause Shift to Anaerobic Metabolism in Listeria monocytogenes and Induce Phenotypes Linked to Antibiotic Tolerance
    Article Snippet: Samples for RNA sequencing were harvested from antibiotic-exposed and control cells at 0 and 3 h and were quenched for 30 min in ice-cold 2% phenol, 38% ethanol, 62% water, to stabilize RNA ( ). .. Total RNA quality and quantity were assessed by an Agilent 2100 Bioanalyser using an RNA 6000 Nano chip and Nanodrop spectrophotometer, respectively.

    Article Title: The Beta-adrenergic agonist, Ractopamine, increases skeletal muscle expression of Asparagine Synthetase as part of an integrated stress response gene program
    Article Snippet: The RNA samples selected for RNA-sequencing were purified using the Qiagen miRNeasy kit, by following the manufacturer’s instructions. .. RNA quantity was measured using a Nanodrop ND-1000 (Nanodrop Technologies, Wilmington, US) and RNA integrity number (RIN) was determined using an Agilent 2100 Bioanalyser (Agilent, Stockport, UK).

    Article Title: Transcriptomic profiling of host-parasite interactions in the microsporidian Trachipleistophora hominis
    Article Snippet: The RNA integrity and concentration was assessed using the Agilent RNA 6000 Nano Kit on the Agilent 2100 BioAnalyser. .. PolyA RNA was isolated from 5 μg of purified total RNA.

    RNA HS Assay:

    Article Title: Nanopore-based detection and characterization of yam viruses
    Article Snippet: Small RNA (sRNA) molecules, including small 21–24 nucleotides (nt) interfering RNAs, were extracted from symptomatic leaves of the water yam sample using a RNAzolRT kit (WAK Chemie, Steinbach, Germany) following the manufacturers protocol (RNAzol®RT Brochure, 2010, Molecular Research Center, Inc. Cincinnati, OH). .. The small RNA fraction was quantified in a Qubit fluorimeter using a Qubit RNA HS Assay Kit (Thermo Fischer Scientific, Waltham, USA) and checked for quality in a bioanalyser 2100 (Agilent). .. RNA passing the quality check was used for library preparation and subjected to high-throughput sequencing on an Illumina “Hi-Seq 2000” instrument using the services of a commercial company (Fasteris SA, Plan-les-Ouates, Switzerland).

    Isolation:

    Article Title: HMA4 expression in tobacco reduces Cd accumulation due to the induction of the apoplastic barrier
    Article Snippet: Paragraph title: RNA isolation ... The quality of RNA used for microarray analysis was checked using an Agilent 2100 Bioanalyser (Agilent Technologies, Santa Clara, CA, USA), according to the manufacturer’s instructions.

    Article Title: Heart function and thoracic aorta gene expression profiling studies of ginseng combined with different herbal medicines in eNOS knockout mice
    Article Snippet: The peri-adventitial, fibro-adipose tissues were carefully isolated. .. Total RNA was extracted using mirVana RNA Isolation Kit (Thermo Scientific, Waltham, USA), and quantified using the NanoDrop ND-2000 (Thermo Scientific, Waltham, USA) and the RNA integrity was evaluated using Agilent Bioanalyser 2100 (Agilent Technologies, Palo Alto, USA). .. Sample labelling, microarray hybridization and washing were successively performed according to the manufacturer’s instructions.

    Article Title: The Intensity of IUGR-Induced Transcriptome Deregulations Is Inversely Correlated with the Onset of Organ Function in a Rat Model
    Article Snippet: This way, we hypothesize that the most drastic cases of IUGR have been considered in the study. .. RNA isolation was performed according to standard protocols using Trizol reagent and controlled (precise integrity checks and sample quantitation) by Agilent bioanalyser 2100. .. Ten micrograms total RNA from a pool of more than 30 fetal tissues from LP group and a pool of more 30 fetal tissues (those displaying the larger weight reduction) for the control group were hybridized to long oligonucleotides microarrays, at the Nimblegen platform in Reykjavik, Iceland, following described protocols.

    Article Title: Full-length transcriptome of Misgurnus anguillicaudatus provides insights into evolution of genus Misgurnus
    Article Snippet: The total RNA of each sample was isolated using TRIzol reagent (TaKaRa, Dalian, China), and then genomic DNA was removed using gDNA eraser (TaKaRa, Dalian, China). .. RNA quality and concentration were determined using NanoDrop 2000 (Thermo Scientific, DE, USA) and Agilent Bioanalyser 2100 (Agilent Technologies, CA, USA), respectively.

    RNA Extraction:

    Article Title: Sublethal Concentrations of Antibiotics Cause Shift to Anaerobic Metabolism in Listeria monocytogenes and Induce Phenotypes Linked to Antibiotic Tolerance
    Article Snippet: Cells were pelleted by centrifugation and stored at -80°C until total RNA extraction using Trizol (Invitrogen) according to . .. Total RNA quality and quantity were assessed by an Agilent 2100 Bioanalyser using an RNA 6000 Nano chip and Nanodrop spectrophotometer, respectively.

    Article Title: Transcriptome analysis of human ageing in male skin shows mid-life period of variability and central role of NF-κB
    Article Snippet: Paragraph title: RNA extraction ... Quantity and quality of RNA was determined using an Agilent 2100 BioAnalyser (Agilent Technologies).

    Article Title: The Promoter Methylation Status and mRNA Expression Levels of CTCF and SIRT6 in Sporadic Breast Cancer
    Article Snippet: Paragraph title: DNA and RNA extraction ... RNA quality was verified using an Agilent Bioanalyser 2100 (Agilent Technologies, Santa Clara, CA).

    Article Title: Nanopore-based detection and characterization of yam viruses
    Article Snippet: Paragraph title: Small RNA extraction and Illumina sequencing ... The small RNA fraction was quantified in a Qubit fluorimeter using a Qubit RNA HS Assay Kit (Thermo Fischer Scientific, Waltham, USA) and checked for quality in a bioanalyser 2100 (Agilent).

    Article Title: Full-length transcriptome of Misgurnus anguillicaudatus provides insights into evolution of genus Misgurnus
    Article Snippet: Paragraph title: Sampling and RNA extraction ... RNA quality and concentration were determined using NanoDrop 2000 (Thermo Scientific, DE, USA) and Agilent Bioanalyser 2100 (Agilent Technologies, CA, USA), respectively.

    Article Title: Identification of superior reference genes for data normalisation of expression studies via quantitative PCR in hybrid roses (Rosa hybrida)
    Article Snippet: Paragraph title: Total RNA extraction and cDNA synthesis ... The RNA quality was assessed by gel electrophoresis and for a subset of samples using a Bioanalyser 2100 lab chip (Agilent, Santa Clara).

    Labeling:

    Article Title: The Intensity of IUGR-Induced Transcriptome Deregulations Is Inversely Correlated with the Onset of Organ Function in a Rat Model
    Article Snippet: RNA isolation was performed according to standard protocols using Trizol reagent and controlled (precise integrity checks and sample quantitation) by Agilent bioanalyser 2100. .. RNA isolation was performed according to standard protocols using Trizol reagent and controlled (precise integrity checks and sample quantitation) by Agilent bioanalyser 2100.

    Purification:

    Article Title: Sialic Acid Utilisation and Synthesis in the Neonatal Rat Revisited
    Article Snippet: Total RNA was then extracted and purified with the RNAdvance tissue kit (Agencourt) through an automated procedure on a Hamilton robotic station. .. After extraction, RNA was quantified with the RiboGreen RNA Quantification Kit (Molecular Probes) and monitored for quality on a Agilent 2100 Bioanalyser (RNA integrity numbers ≧8 for high quality).

    Article Title: Sublethal Concentrations of Antibiotics Cause Shift to Anaerobic Metabolism in Listeria monocytogenes and Induce Phenotypes Linked to Antibiotic Tolerance
    Article Snippet: Paragraph title: RNA Purification ... Total RNA quality and quantity were assessed by an Agilent 2100 Bioanalyser using an RNA 6000 Nano chip and Nanodrop spectrophotometer, respectively.

    Article Title: The Beta-adrenergic agonist, Ractopamine, increases skeletal muscle expression of Asparagine Synthetase as part of an integrated stress response gene program
    Article Snippet: The RNA samples selected for RNA-sequencing were purified using the Qiagen miRNeasy kit, by following the manufacturer’s instructions. .. RNA quantity was measured using a Nanodrop ND-1000 (Nanodrop Technologies, Wilmington, US) and RNA integrity number (RIN) was determined using an Agilent 2100 Bioanalyser (Agilent, Stockport, UK).

    Article Title: Transcriptomic profiling of host-parasite interactions in the microsporidian Trachipleistophora hominis
    Article Snippet: An additional cleanup step using the GeneJet RNA purification kit (Thermo Scientific) was added to remove residual organic solvents from the purified total RNA. .. The RNA integrity and concentration was assessed using the Agilent RNA 6000 Nano Kit on the Agilent 2100 BioAnalyser.

    Article Title: Transcriptome analysis of human ageing in male skin shows mid-life period of variability and central role of NF-κB
    Article Snippet: Samples were dissected with scalpel in 0.5 ml of TRIzol reagent (Invitrogen) then homogenized on ice using a hand-held rotor-stator homogenizer (TissueRuptor, Qiagen) with disposable probes, in 10 s bursts for a total of 60 s. Samples were then processed with a standard TRIzol/chloroform and RNA Easy kit (Qiagen) purification protocol as per the manufacturer’s instructions. .. Quantity and quality of RNA was determined using an Agilent 2100 BioAnalyser (Agilent Technologies).

    Article Title: Expression of Genes for Drug Transporters in the Human Female Genital Tract and Modulatory Effect of Antiretroviral Drugs
    Article Snippet: Paragraph title: Purification of total RNA, cDNA synthesis and quantitative PCR ... Integrity and quantification of RNA were evaluated using the Agilent RNA 6000 Nano Kit (Agilent Technologies, Milan, Italy) on the Agilent 2100 Bioanalyser (Agilent Technologies).

    Article Title: Accurate diagnosis of mismatch repair deficiency in colorectal cancer using high-quality DNA samples from cultured stem cells
    Article Snippet: Three electrophoretic runs were performed for each sample (BAT25 /D2S123, D5S346 /D17S250 , and BAT26 ) according to the standard protocol [ ] ( ). .. Amplified DNA fragments were purified with QIAquick PCR Purification Kit (Qiagen), applied on the DNA LabChip of the Agilent DNA 1000 Kit (Agilent Technologies, Santa Clara, CA, USA), and analyzed with an Agilent 2100 Bioanalyser instrument (Agilent Technologies) according to the previous protocol [ ]. .. The electropherogram of each cancer spheroid line was overlaid with that of the normal epithelial spheroid line derived from the same patient.

    Polymerase Chain Reaction:

    Article Title: Accurate diagnosis of mismatch repair deficiency in colorectal cancer using high-quality DNA samples from cultured stem cells
    Article Snippet: Three electrophoretic runs were performed for each sample (BAT25 /D2S123, D5S346 /D17S250 , and BAT26 ) according to the standard protocol [ ] ( ). .. Amplified DNA fragments were purified with QIAquick PCR Purification Kit (Qiagen), applied on the DNA LabChip of the Agilent DNA 1000 Kit (Agilent Technologies, Santa Clara, CA, USA), and analyzed with an Agilent 2100 Bioanalyser instrument (Agilent Technologies) according to the previous protocol [ ]. .. The electropherogram of each cancer spheroid line was overlaid with that of the normal epithelial spheroid line derived from the same patient.

    Article Title: Molecular and proteomic analyses highlight the importance of ubiquitination for the stress resistance, metabolic adaptation, morphogenetic regulation and virulence of Candida albicans
    Article Snippet: RNA was extracted with Trizol (GibcoBRL, Grand Island, NY, USA) as described previously , and RNA integrity assayed on an Agilent Bioanalyser 2100 (Stockport, UK). .. Briefly, for the target transcripts UBI4 and ACT1 , probes were chosen using the ProbeFinder Software Version 2.45 (Roche, http://www.universalprobelibrary.com ).

    Article Title: Full-length transcriptome of Misgurnus anguillicaudatus provides insights into evolution of genus Misgurnus
    Article Snippet: RNA quality and concentration were determined using NanoDrop 2000 (Thermo Scientific, DE, USA) and Agilent Bioanalyser 2100 (Agilent Technologies, CA, USA), respectively. .. RNA quality and concentration were determined using NanoDrop 2000 (Thermo Scientific, DE, USA) and Agilent Bioanalyser 2100 (Agilent Technologies, CA, USA), respectively.

    Construct:

    Article Title: Full-length transcriptome of Misgurnus anguillicaudatus provides insights into evolution of genus Misgurnus
    Article Snippet: RNA quality and concentration were determined using NanoDrop 2000 (Thermo Scientific, DE, USA) and Agilent Bioanalyser 2100 (Agilent Technologies, CA, USA), respectively. .. After a round of PCR amplification, the amplified cDNA was size selected into different size fractions to prevent preferential small template sequencing, using the Blue Pippin (Sage Science; MA, USA).

    Mouse Assay:

    Article Title: Heart function and thoracic aorta gene expression profiling studies of ginseng combined with different herbal medicines in eNOS knockout mice
    Article Snippet: Total RNA was extracted using mirVana RNA Isolation Kit (Thermo Scientific, Waltham, USA), and quantified using the NanoDrop ND-2000 (Thermo Scientific, Waltham, USA) and the RNA integrity was evaluated using Agilent Bioanalyser 2100 (Agilent Technologies, Palo Alto, USA). .. Total RNA was extracted using mirVana RNA Isolation Kit (Thermo Scientific, Waltham, USA), and quantified using the NanoDrop ND-2000 (Thermo Scientific, Waltham, USA) and the RNA integrity was evaluated using Agilent Bioanalyser 2100 (Agilent Technologies, Palo Alto, USA).

    Chromatin Immunoprecipitation:

    Article Title: Sublethal Concentrations of Antibiotics Cause Shift to Anaerobic Metabolism in Listeria monocytogenes and Induce Phenotypes Linked to Antibiotic Tolerance
    Article Snippet: Cells were pelleted by centrifugation and stored at -80°C until total RNA extraction using Trizol (Invitrogen) according to . .. Total RNA quality and quantity were assessed by an Agilent 2100 Bioanalyser using an RNA 6000 Nano chip and Nanodrop spectrophotometer, respectively. .. To reduce the amount of rRNA reads for the RNA sequencing, we isolated mRNA from good quality total RNA (RIN 9.8-10) using the MICROBExpress kit (Ambion AM1905) according to the manufacture’s protocol.

    Article Title: Identification of superior reference genes for data normalisation of expression studies via quantitative PCR in hybrid roses (Rosa hybrida)
    Article Snippet: The RNA concentration was assessed spectrophotometrically at 260 nm and was checked for purity by determining the OD 260 nm/280 nm and the OD 260 nm/230 nm ratios, respectively. .. The RNA quality was assessed by gel electrophoresis and for a subset of samples using a Bioanalyser 2100 lab chip (Agilent, Santa Clara). .. For each sample, 300 ng of total RNA was reverse-transcribed using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Austin) and 1 μg of an oligo-(dT)18 Primer (Fermentas, St. Leon-Rot) according to the manufacturer's instructions.

    Software:

    Article Title: Molecular and proteomic analyses highlight the importance of ubiquitination for the stress resistance, metabolic adaptation, morphogenetic regulation and virulence of Candida albicans
    Article Snippet: RNA was extracted with Trizol (GibcoBRL, Grand Island, NY, USA) as described previously , and RNA integrity assayed on an Agilent Bioanalyser 2100 (Stockport, UK). .. Real-time PCR was performed in triplicate in optical multiwall plate 384 using the LightCycler 480 Probes Master (Roche Applied Science; Burgess Hill, UK) as per the manufacturer's guidelines.

    Article Title: The Intensity of IUGR-Induced Transcriptome Deregulations Is Inversely Correlated with the Onset of Organ Function in a Rat Model
    Article Snippet: RNA isolation was performed according to standard protocols using Trizol reagent and controlled (precise integrity checks and sample quantitation) by Agilent bioanalyser 2100. .. A total of 390,000 oligonucleotides representing 23,456 transcripts from the rat genome were hybridized with fluorescently labeled cDNAs from each fetal tissue (LP group vs control group).

    Multiplex Assay:

    Article Title: Accurate diagnosis of mismatch repair deficiency in colorectal cancer using high-quality DNA samples from cultured stem cells
    Article Snippet: Microsatellite DNA fragments were amplified from spheroid DNA samples using Multiplex PCR Kit (Qiagen) and primer pairs for the Bethesda panel markers [ , ] ( ). .. Amplified DNA fragments were purified with QIAquick PCR Purification Kit (Qiagen), applied on the DNA LabChip of the Agilent DNA 1000 Kit (Agilent Technologies, Santa Clara, CA, USA), and analyzed with an Agilent 2100 Bioanalyser instrument (Agilent Technologies) according to the previous protocol [ ].

    Agarose Gel Electrophoresis:

    Article Title: The Promoter Methylation Status and mRNA Expression Levels of CTCF and SIRT6 in Sporadic Breast Cancer
    Article Snippet: DNA and RNA integrity was checked by agarose gel electrophoresis. .. RNA quality was verified using an Agilent Bioanalyser 2100 (Agilent Technologies, Santa Clara, CA).

    In Vitro:

    Article Title: Sialic Acid Utilisation and Synthesis in the Neonatal Rat Revisited
    Article Snippet: After extraction, RNA was quantified with the RiboGreen RNA Quantification Kit (Molecular Probes) and monitored for quality on a Agilent 2100 Bioanalyser (RNA integrity numbers ≧8 for high quality). .. All cRNA targets were synthesized, labelled and purified according to the Illumina TotalPrep RNA amplification protocol (Applied Biosystems/Ambion) using the Hamilton robotic station.

    Article Title: Identification of ovule transcripts from the Apospory-Specific Genomic Region (ASGR)-carrier chromosome
    Article Snippet: With total RNA as starting material, mRNA was amplified by T7-based in vitro transcription following the manual of TargetAmp™2-Round aRNA Amplification Kit 2.0 (Epicentre, Madison, WI). .. Size-range and quantity of ds-cDNA were also analyzed by both gel electrophoresis and using the Agilent 2100 Bioanalyser before submitting the samples for sequencing.

    Quantitation Assay:

    Article Title: The Intensity of IUGR-Induced Transcriptome Deregulations Is Inversely Correlated with the Onset of Organ Function in a Rat Model
    Article Snippet: This way, we hypothesize that the most drastic cases of IUGR have been considered in the study. .. RNA isolation was performed according to standard protocols using Trizol reagent and controlled (precise integrity checks and sample quantitation) by Agilent bioanalyser 2100. .. Ten micrograms total RNA from a pool of more than 30 fetal tissues from LP group and a pool of more 30 fetal tissues (those displaying the larger weight reduction) for the control group were hybridized to long oligonucleotides microarrays, at the Nimblegen platform in Reykjavik, Iceland, following described protocols.

    Spectrophotometry:

    Article Title: A Splice Mutation in the PHKG1 Gene Causes High Glycogen Content and Low Meat Quality in Pig Skeletal Muscle
    Article Snippet: Total RNA was extracted from longissimus dorsi muscle samples of 497 F2 animals using Trizol (Invitrogen). .. RNA quantity and integrity were assessed using a NanoDrop ND-1000 Spectrophotometer (Thermo Fisher Scientific) and a 2100 Bioanalyser (Agilent). .. Genome-wide transcripts were assayed by digital gene expression (DGE) system and data processing was conducted as described previously , .

    Article Title: The Anti-Proliferative Effects of Enterolactone in Prostate Cancer Cells: Evidence for the Role of DNA Licencing Genes, mi-R106b Cluster Expression, and PTEN Dosage
    Article Snippet: The NucleoSpin® miRNA kit (Macherey-Nagel, Düren, Germany) was used to extract large and small RNA in separate fractions from each of the samples according to the manufacturer’s instructions. .. RNA quantity and integrity was determined based on A260:280 and A260:230 nm ratios using a NanoDrop 1000 spectrophotometer (Thermo Fisher Scientific, Melbourne, Australia) and a Agilent 2100 bioanalyser (Agilent, Santa Clara, CA, USA). .. Only RNA with both absorbance ratios of 1.8 to 2.1 and with a RIN value of 9 or greater were considered to be of sufficient quality and integrity.

    Article Title: HMA4 expression in tobacco reduces Cd accumulation due to the induction of the apoplastic barrier
    Article Snippet: All RNA samples were quantified at A 260 using a Nanodrop spectrophotometer ND100 (Nanodrop, Wilimington, DE, USA). .. The quality of RNA used for microarray analysis was checked using an Agilent 2100 Bioanalyser (Agilent Technologies, Santa Clara, CA, USA), according to the manufacturer’s instructions.

    Article Title: Sublethal Concentrations of Antibiotics Cause Shift to Anaerobic Metabolism in Listeria monocytogenes and Induce Phenotypes Linked to Antibiotic Tolerance
    Article Snippet: Cells were pelleted by centrifugation and stored at -80°C until total RNA extraction using Trizol (Invitrogen) according to . .. Total RNA quality and quantity were assessed by an Agilent 2100 Bioanalyser using an RNA 6000 Nano chip and Nanodrop spectrophotometer, respectively. .. To reduce the amount of rRNA reads for the RNA sequencing, we isolated mRNA from good quality total RNA (RIN 9.8-10) using the MICROBExpress kit (Ambion AM1905) according to the manufacture’s protocol.

    Sampling:

    Article Title: Full-length transcriptome of Misgurnus anguillicaudatus provides insights into evolution of genus Misgurnus
    Article Snippet: Paragraph title: Sampling and RNA extraction ... RNA quality and concentration were determined using NanoDrop 2000 (Thermo Scientific, DE, USA) and Agilent Bioanalyser 2100 (Agilent Technologies, CA, USA), respectively.

    Concentration Assay:

    Article Title: Transcriptomic profiling of host-parasite interactions in the microsporidian Trachipleistophora hominis
    Article Snippet: An additional cleanup step using the GeneJet RNA purification kit (Thermo Scientific) was added to remove residual organic solvents from the purified total RNA. .. The RNA integrity and concentration was assessed using the Agilent RNA 6000 Nano Kit on the Agilent 2100 BioAnalyser. .. PolyA RNA was isolated from 5 μg of purified total RNA.

    Article Title: Nutrigenomic and Nutritional Analyses Reveal the Effects of Pelleted Feeds on Asian Seabass (Lates calcarifer)
    Article Snippet: RNA concentration was determined using NanoDrop 1000 (NanoDrop Technologies). .. The RNA integrity number (RIN) of extracted total RNA was determined by using Agilent 2100 Bioanalyser (Agilent Technologies, Nærum, Denmark).

    Article Title: Full-length transcriptome of Misgurnus anguillicaudatus provides insights into evolution of genus Misgurnus
    Article Snippet: The total RNA of each sample was isolated using TRIzol reagent (TaKaRa, Dalian, China), and then genomic DNA was removed using gDNA eraser (TaKaRa, Dalian, China). .. RNA quality and concentration were determined using NanoDrop 2000 (Thermo Scientific, DE, USA) and Agilent Bioanalyser 2100 (Agilent Technologies, CA, USA), respectively. .. Equal amounts of the total RNA from each of the 12 tissues were pooled to generate one sample for library preparation.

    Article Title: Identification of superior reference genes for data normalisation of expression studies via quantitative PCR in hybrid roses (Rosa hybrida)
    Article Snippet: The RNA concentration was assessed spectrophotometrically at 260 nm and was checked for purity by determining the OD 260 nm/280 nm and the OD 260 nm/230 nm ratios, respectively. .. The RNA quality was assessed by gel electrophoresis and for a subset of samples using a Bioanalyser 2100 lab chip (Agilent, Santa Clara).

    Marker:

    Article Title: Accurate diagnosis of mismatch repair deficiency in colorectal cancer using high-quality DNA samples from cultured stem cells
    Article Snippet: Amplified DNA fragments were purified with QIAquick PCR Purification Kit (Qiagen), applied on the DNA LabChip of the Agilent DNA 1000 Kit (Agilent Technologies, Santa Clara, CA, USA), and analyzed with an Agilent 2100 Bioanalyser instrument (Agilent Technologies) according to the previous protocol [ ]. .. The electropherogram of each cancer spheroid line was overlaid with that of the normal epithelial spheroid line derived from the same patient.

    Lysis:

    Article Title: Sialic Acid Utilisation and Synthesis in the Neonatal Rat Revisited
    Article Snippet: Brain and colon tissue samples (approximately 10 mg wet weight) were disrupted and homogenized in lysis buffer using a FastPrep instrument and lysing tubes containing ceramic beads (MP Biomedicals). .. After extraction, RNA was quantified with the RiboGreen RNA Quantification Kit (Molecular Probes) and monitored for quality on a Agilent 2100 Bioanalyser (RNA integrity numbers ≧8 for high quality).

    Article Title: Expression of Genes for Drug Transporters in the Human Female Genital Tract and Modulatory Effect of Antiretroviral Drugs
    Article Snippet: Biopsies were homogenized in RA1 Lysis Buffer using 5 mm stainless steel beads in the Tissues Lyser apparatus (Qiagen s.r.l., Milan, Italy). .. Integrity and quantification of RNA were evaluated using the Agilent RNA 6000 Nano Kit (Agilent Technologies, Milan, Italy) on the Agilent 2100 Bioanalyser (Agilent Technologies).

    T-Test:

    Article Title: The Intensity of IUGR-Induced Transcriptome Deregulations Is Inversely Correlated with the Onset of Organ Function in a Rat Model
    Article Snippet: RNA isolation was performed according to standard protocols using Trizol reagent and controlled (precise integrity checks and sample quantitation) by Agilent bioanalyser 2100. .. Image analysis was performed with the NimbleScan software (Nimblegen), and feature intensities were exported as .pair files.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Agilent technologies 2100 bioanalyser rna 6000 nano labchip kit agilent technologies santa clara usa
    2100 Bioanalyser Rna 6000 Nano Labchip Kit Agilent Technologies Santa Clara Usa, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/2100 bioanalyser rna 6000 nano labchip kit agilent technologies santa clara usa/product/Agilent technologies
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    2100 bioanalyser rna 6000 nano labchip kit agilent technologies santa clara usa - by Bioz Stars, 2019-12
    99/100 stars
      Buy from Supplier

    Image Search Results