Structured Review

Promega 20u milliliter rnasin
20u Milliliter Rnasin, supplied by Promega, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/20u milliliter rnasin/product/Promega
Average 94 stars, based on 2 article reviews
Price from $9.99 to $1999.99
20u milliliter rnasin - by Bioz Stars, 2020-03
94/100 stars

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Related Articles

Centrifugation:

Article Title: Impact of two neighbouring ribosomal protein clusters on biogenesis factor binding and assembly of yeast late small ribosomal subunit precursors
Article Snippet: Cells were thawed on ice, washed once in 5 milliliters of buffer A200 (200 mM potassium chloride, 20mM Tris pH8, 5mM magnesium acetate, 1 mM phenylmethylsulfonyl fluoride, 2 mM benzamidin) and were then suspended in 1.5 milliliters buffer A200 with 20U/milliliter RNasin (Promega) and 1mM dithiothreitol. .. The crude extract was cleared by two consecutive centrifugation steps for 5 and 10 minutes at 15000g at 4°C.

Mutagenesis:

Article Title: Impact of two neighbouring ribosomal protein clusters on biogenesis factor binding and assembly of yeast late small ribosomal subunit precursors
Article Snippet: Cells were thawed on ice, washed once in 5 milliliters of buffer A200 (200 mM potassium chloride, 20mM Tris pH8, 5mM magnesium acetate, 1 mM phenylmethylsulfonyl fluoride, 2 mM benzamidin) and were then suspended in 1.5 milliliters buffer A200 with 20U/milliliter RNasin (Promega) and 1mM dithiothreitol. .. The protein concentration of the resulting supernatant was determined by Bradford protein assay (Bio-Rad) and concentrations were adjusted with buffer A200 (with 20U/milliliter RNasin and 1mM dithiothreitol) to match among extracts of mutant and wildtype strains included in the respective analysis.

Bradford Protein Assay:

Article Title: Impact of two neighbouring ribosomal protein clusters on biogenesis factor binding and assembly of yeast late small ribosomal subunit precursors
Article Snippet: Cells were thawed on ice, washed once in 5 milliliters of buffer A200 (200 mM potassium chloride, 20mM Tris pH8, 5mM magnesium acetate, 1 mM phenylmethylsulfonyl fluoride, 2 mM benzamidin) and were then suspended in 1.5 milliliters buffer A200 with 20U/milliliter RNasin (Promega) and 1mM dithiothreitol. .. The protein concentration of the resulting supernatant was determined by Bradford protein assay (Bio-Rad) and concentrations were adjusted with buffer A200 (with 20U/milliliter RNasin and 1mM dithiothreitol) to match among extracts of mutant and wildtype strains included in the respective analysis.

Protein Concentration:

Article Title: Impact of two neighbouring ribosomal protein clusters on biogenesis factor binding and assembly of yeast late small ribosomal subunit precursors
Article Snippet: Cells were thawed on ice, washed once in 5 milliliters of buffer A200 (200 mM potassium chloride, 20mM Tris pH8, 5mM magnesium acetate, 1 mM phenylmethylsulfonyl fluoride, 2 mM benzamidin) and were then suspended in 1.5 milliliters buffer A200 with 20U/milliliter RNasin (Promega) and 1mM dithiothreitol. .. The protein concentration of the resulting supernatant was determined by Bradford protein assay (Bio-Rad) and concentrations were adjusted with buffer A200 (with 20U/milliliter RNasin and 1mM dithiothreitol) to match among extracts of mutant and wildtype strains included in the respective analysis.

Affinity Purification:

Article Title: Impact of two neighbouring ribosomal protein clusters on biogenesis factor binding and assembly of yeast late small ribosomal subunit precursors
Article Snippet: Paragraph title: Affinity purification of TAP tagged ribosome biogenesis factors for subsequent RNA analyses ... Cells were thawed on ice, washed once in 5 milliliters of buffer A200 (200 mM potassium chloride, 20mM Tris pH8, 5mM magnesium acetate, 1 mM phenylmethylsulfonyl fluoride, 2 mM benzamidin) and were then suspended in 1.5 milliliters buffer A200 with 20U/milliliter RNasin (Promega) and 1mM dithiothreitol.

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    Promega 20u milliliter rnasin
    20u Milliliter Rnasin, supplied by Promega, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/20u milliliter rnasin/product/Promega
    Average 94 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    20u milliliter rnasin - by Bioz Stars, 2020-03
    94/100 stars
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