qiagen multiplex pcr kit  (Qiagen)


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    Name:
    QIAGEN Multiplex PCR Kit
    Description:
    For highly specific and sensitive multiplex PCR without optimization requirements Kit contents Qiagen Multiplex PCR Kit 100 x 50L rxns Genomic DNA and cDNA Sample PCR Amplification Reaction 5 3 Exonuclease Enzyme Activity Easy to use and Cost effective For Highly Specific and Sensitive Multiplex PCR Without Optimization Requirements Includes 2x Qiagen Multiplex PCR Master Mix Providing a Final Concentration of 3mM MgCl2 3 x 0 85mL 5x Q Solution 1 x 2 0mL RNase free Water 2 x 1 7mL Benefits No optimization required High specificity and sensitivity with a built in hot start Highly suited for many types of multiplex PCR applications Easy to use and cost effectiv
    Catalog Number:
    206143
    Price:
    284
    Category:
    QIAGEN Multiplex PCR Kit
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    Structured Review

    Qiagen qiagen multiplex pcr kit
    QIAGEN Multiplex PCR Kit
    For highly specific and sensitive multiplex PCR without optimization requirements Kit contents Qiagen Multiplex PCR Kit 100 x 50L rxns Genomic DNA and cDNA Sample PCR Amplification Reaction 5 3 Exonuclease Enzyme Activity Easy to use and Cost effective For Highly Specific and Sensitive Multiplex PCR Without Optimization Requirements Includes 2x Qiagen Multiplex PCR Master Mix Providing a Final Concentration of 3mM MgCl2 3 x 0 85mL 5x Q Solution 1 x 2 0mL RNase free Water 2 x 1 7mL Benefits No optimization required High specificity and sensitivity with a built in hot start Highly suited for many types of multiplex PCR applications Easy to use and cost effectiv
    https://www.bioz.com/result/qiagen multiplex pcr kit/product/Qiagen
    Average 90 stars, based on 1105 article reviews
    Price from $9.99 to $1999.99
    qiagen multiplex pcr kit - by Bioz Stars, 2020-01
    90/100 stars

    Images

    1) Product Images from "An efficient technique for primer development and application that integrates fluorescent labeling and multiplex PCR 1"

    Article Title: An efficient technique for primer development and application that integrates fluorescent labeling and multiplex PCR 1

    Journal: Applications in Plant Sciences

    doi: 10.3732/apps.1300027

    (see p. 4).The effect of multiplex PCR thermocycler programs during the dual labeling process on the amplitude and signal strength of fragment peaks. Shown are electropherograms from microsatellite loci KA16, ch0203b, and ch01h01 in the ‘Redspire’ cultivar of Pyrus calleryana when the thermocycler cycling program was that (A) suggested by Schuelke (2000) as two series of cycles or recommended by the QIAGEN Multiplexing Kit for (B) 35 cycles, (C) 40 cycles, (D) 45 cycles, and (E) 50 cycles. For comparison, the same samples are presented (F) following traditional multiplexed PCR with prelabeled primers and (G) when analyzed individually with a single prelabeled primer pair; in both of these cases, the normal QIAGEN-recommended protocol was used with 35 cycles.
    Figure Legend Snippet: (see p. 4).The effect of multiplex PCR thermocycler programs during the dual labeling process on the amplitude and signal strength of fragment peaks. Shown are electropherograms from microsatellite loci KA16, ch0203b, and ch01h01 in the ‘Redspire’ cultivar of Pyrus calleryana when the thermocycler cycling program was that (A) suggested by Schuelke (2000) as two series of cycles or recommended by the QIAGEN Multiplexing Kit for (B) 35 cycles, (C) 40 cycles, (D) 45 cycles, and (E) 50 cycles. For comparison, the same samples are presented (F) following traditional multiplexed PCR with prelabeled primers and (G) when analyzed individually with a single prelabeled primer pair; in both of these cases, the normal QIAGEN-recommended protocol was used with 35 cycles.

    Techniques Used: Multiplex Assay, Polymerase Chain Reaction, Labeling, Multiplexing

    Related Articles

    Diagnostic Assay:

    Article Title: Clinical Phenotypes Associated to Engrailed 2 Gene Alterations in a Series of Neuropediatric Patients
    Article Snippet: The molecular analysis was carried out by multiplex (QIAGEN Multiplex PCR Kit, catalog number 206145) using the primers described in Table , and the results visualized with QIAxcel System (capillary electrophoresis device, catalog number 9001421, Qiagen, Madrid). .. The results of the genetic study distributed the patients in three groups: 12 patients presented mutations in EN2 gene (EN2-g), n = 12, 20 patients presented mutations in other genes studied in the panel used in our laboratory facilities to detect genetic anomalies in patients with diagnostic of cortical dysplasia (LIS1[PAFAH1B1], PTAFR, PAFAH1B2, PAFAH1B3, FGF8, PAX2, D17S79, D17S1866 , and D17S5 , Table ), but not in EN2 , (OG-g); and 62 patients showing neither alterations in genes of the panel or EN2 ( Table ).

    Amplification:

    Article Title: Role of Pneumocystis jirovecii in patients with different pulmonary underlying condition using a nested-PCR
    Article Snippet: The external primers pAZ102-E and pAZ102-H to mtLSUrRNA P. jirovecii gene amplification were used in the first amplification round which produced a 346 bp amplicon, being included in the same reaction as an internal control 16s rRNA gene amplification primers (U1 and U2), which produced a 996 bp product [ ]. .. Each PCR reaction contained 5 μL of the extraction product, 12.5 μL QIAGEN Multiplex PCR MasterMix and 0.2 μM concentration of each primer in a total volume of 25 μL (QIAGEN Multiplex PCR kit,Qiagen, Hilden, Germany);5 μL of the first PCR product was used as the DNA template for the second PCR reaction.

    Article Title: Landscape features and helminth co-infection shape bank vole immunoheterogeneity, with consequences for Puumala virus epidemiology
    Article Snippet: PCR was performed on anEPgradient S MasterCycler (Qiagen, Venlo, Netherlands), in a final volume of25 μl containing 1 × Qiagen Multiplex PCR Kit,0.2 μ M of each primer and 2 μl of template cDNA diluted1:5. .. Based on the cDNA consensus sequencesobtained for these two M. glareolus samples (GenBank AN: ), we designed the following specific primers:Mx2q2-F (5′-TCAGAGAGAAGGAAGCCGA-3′) and Mx2q2-R(5′-GAGATGCGGTTGTGAGC-3′), binding to exons 13 and 14,respectively, (amplicon size 142 bp).

    Article Title: MTHFRC677T and A1298C Genotypes and Haplotypes in Slovenian Couples with Unexplained Infertility Problems and in Embryonic Tissues from Spontaneous Abortions
    Article Snippet: Two reactions were performed per sample with the QIAGEN Multiplex PCR Kit (Cat. #206143; Qiagen) by amplifying one allele of each polymorphism (two duplex reactions). .. In reaction 1, alleles 677T and 1298A were amplified with MTHFR 677T-F (5′-GAA GGT GTC TGC GGG CGT-3′), MTHFR C677TR (5′-AGC AAC GCT GTG CAA GTT CTG-3′), MTHFR 1298A-F (5′-AGG AGC TGA CCA GTG AGG A-3′) and MTHFR 1298C-R (5′-TTC TCC CTT TGC CAT GTC C-3′).

    Article Title: Evidence of Habitat Structuring Aedes albopictus Populations in R?union Island
    Article Snippet: .. Genomic (10 ng) DNA was used for amplification with the QIAGEN multiplex PCR Master Mix kit (ref. 206145) according to the manufacturer's instructions in a final volume of 15 µL. .. One of each pair of primers was fluorescently end-labelled with the fluorochromes NED, VIC, PET or FAM.

    Article Title: All-trans retinoic acid impairs the vasculogenic mimicry formation ability of U87 stem-like cells through promoting differentiation
    Article Snippet: PCR was performed in a 50 μ l reaction volume containing 1 μ l complementary DNA, 6X reaction buffer, 0.75 mM MgCl2 , 0.04 mM deoxynucleotide mix, 0.2 pmol/μ l forward primer, 0.2 pmol/μ l reverse primer and 1 Unit Taq polymerase (Qiagen Multiplex PCR kit, cat. no. 206143; Qiagen, Inc., Valencia, CA, USA) for 35 cycles of 30 sec at 94°C, 58°C and 72°C using a Life ECO Thermal Cycler (BYQ6078; Bioer Technology Co., Ltd., Hangzhou, China). .. The primers used for amplification were as follows: Vascular endothelial growth factor (VEGF) sense, 5′-CAGCTACTGCCATCCAATC-3′ and antisense, 5′-CAAATGCTTTCTCCGCTCTG-3′ (313 bp); and GAPDH sense, 5′-TGCCAGTGGTAATACGATT-3′ and antisense, 5′-TAGGAATACTGCCATCACAA-3′ (458 bp).

    Article Title: Germline BRCA1 mutations increase prostate cancer risk
    Article Snippet: Using Qiagen multiplex PCR kit 206145 (Qiagen, Hilden, Germany), the 29 primer pairs were grouped into four multiplexs (3 × 7 multiplexes and 1 × 6 multiplex) and two singleton reactions. .. Multiplex ligation-dependent probe amplification was performed on a subset of 460 samples using a modified protocol of the SALSA MLPA kit P002-C2 (MRC-Holland, Amsterdam, The Netherlands).

    Article Title: Serum Interleukin-18 and Its Gene Haplotypes Profile as Predictors in Patients with Diabetic Nephropathy
    Article Snippet: PCR reactions were carried on a thermocycler (Bio-Rad, USA) using 2X master mix (Qiagen, Cat No. 206143 Valencia, USA) according to the manufacturer’s instructions. .. The PCR amplified products were run on 1.5% agarose gel and the bands corresponding to the predicted size were cut and purified using the gel extraction kit (QIA quick columns, Qiagen, Cat No. 28104, Valencia, USA) following the manufacturer’s instructions.

    Article Title: Does host socio-spatial behavior lead to a fine-scale spatial genetic structure in its associated parasites?
    Article Snippet: .. For each sample, seven microsatellites (Hcms25, Hcms27, Hcms33, Hcms36, Hcms40, Hcms22co3 and Hcms8a20 , see [ , ], three multiplexes, see Supplementary Table 1 ) were amplified through polymerase chain reaction (PCR) in a final volume of 15 μL composed of QIAGEN Multiplex PCR kit Mastermix (ref. 206145), 40 nM of each primer, and 2 μL of DNA solution. .. PCR cycles consisted of 15 min of activation (95 °C), followed by 40 cycles of denaturation (30 s, 94 °C), annealing (1 min 30 s at primer-specific annealing temperature, see Supplementary Data 2 ) and extension (1 min, 72 °C).

    Article Title: The nasty neighbour in the striped mouse (Rhabdomys pumilio) steals paternity and elicits aggression
    Article Snippet: .. We used 9 polymorphic microsatellite loci from the house mouse genome (Chr13_1, Chr1_12, Chr1_21, Chr2_3, Chr7_64, D3Mit211, Chr11_81, Chr19_18, Chr5_38), primarily from [ ], and amplified them for all individuals in two multiplexes using the Qiagen PCR-Multiplex-Kit with a final concentration of 0.1/0.2 μM primer for 35 cycles at an annealing temperature of 60°C). ..

    Positive Control:

    Article Title: Role of Pneumocystis jirovecii in patients with different pulmonary underlying condition using a nested-PCR
    Article Snippet: Each PCR reaction contained 5 μL of the extraction product, 12.5 μL QIAGEN Multiplex PCR MasterMix and 0.2 μM concentration of each primer in a total volume of 25 μL (QIAGEN Multiplex PCR kit,Qiagen, Hilden, Germany);5 μL of the first PCR product was used as the DNA template for the second PCR reaction. .. During each PCR, a positive control (a BAL fluid sample from a patient with PJP, P. jirovecii positive immunofluorescence assay) and ultra-pure water as the negative control were used.

    Article Title: Prevalence and antibiotic susceptibility patterns of enteric bacterial pathogens in human and non-human sources in an urban informal settlement in Cape Town, South Africa
    Article Snippet: The presence of the fimbrial adhesion gene (daaC) for diffusely adherent E. coli (DAEC), the anti-aggregation protein transporter gene (aat) for enteroaggregative E. coli (EAggEC) heat-stable (ST ) and heat-labile (LT ) enterotoxin genes for enterotoxigenic E. coli (ETEC), the invasive plasmid antigen (ipa ) gene for enteroinvasive E. coli (EIEC), the bundle-forming pili (bfp ) gene for typical enteropathogenic E. coli (EPEC) and the intimin coding gene (eae ) for EPEC were determined using end-point PCR on an Applied Biosystems™ 2720 Thermal Cycler platform using the QIAGEN PCR-multiplex kit (QIAGEN GmbH, Hilden, Germany) followed by agarose gel detection as previously described [ ], using primers as shown in Table (Inqaba Biotec Laboratory, South Africa). .. The positive control strains that were used for the different diarrheic E. coli pathotypes are shown below (Table ) ([ ]): Similarly, sample TSB enrichment broths from all sources were subjected to nucleic acid extraction using the MagNApure bacterial/fungal DNA extraction kit on a MagNApure LC instrument (Roche Diagnostics, Industriestrasse, Switzerland).

    Polymerase Chain Reaction:

    Article Title: A High-Speed Congenic Strategy Using First-Wave Male Germ Cells
    Article Snippet: .. PCR execution was performed using the QIAGEN Multiplex PCR kit (#206143; QIAGEN GmbH) and the length polymorphism was detected by agarose gel electrophoresis. .. Map locations and primer sequences of the microsatellite loci were used according to the Mouse Genome Informatics (MGI) of the Jackson Laboratory, USA, and Mouse Microsatellite Data Base of Japan (MMDBJ).

    Article Title: Role of Pneumocystis jirovecii in patients with different pulmonary underlying condition using a nested-PCR
    Article Snippet: .. Each PCR reaction contained 5 μL of the extraction product, 12.5 μL QIAGEN Multiplex PCR MasterMix and 0.2 μM concentration of each primer in a total volume of 25 μL (QIAGEN Multiplex PCR kit,Qiagen, Hilden, Germany);5 μL of the first PCR product was used as the DNA template for the second PCR reaction. .. The PCR products were analysed by electrophoresis on 1% agarose gel stained with RedSafe TM Nucleic Acid Staining Solution (iNtRON Biotechnology Inc., Sungnam, Kyungki-Do, Republic of Korea) ( ).

    Article Title: Landscape features and helminth co-infection shape bank vole immunoheterogeneity, with consequences for Puumala virus epidemiology
    Article Snippet: .. PCR was performed on anEPgradient S MasterCycler (Qiagen, Venlo, Netherlands), in a final volume of25 μl containing 1 × Qiagen Multiplex PCR Kit,0.2 μ M of each primer and 2 μl of template cDNA diluted1:5. .. Cycling conditions were as follows: initial denaturation at 95 °C for15 min followed by 40 cycles of denaturation at 94 °C for 30 s,annealing at 50 °C for 30 s and elongation at 72 °C for60 s and a final extension phase at 72 °C for 10 min. Sequencesfor both strands were obtained for each sample (Eurofins MWG Synthesis GmbH, Ebersberg,Germany) with the MxUniv2-F and MxUniv2-R primers.

    Article Title: Prevalence and antibiotic susceptibility patterns of enteric bacterial pathogens in human and non-human sources in an urban informal settlement in Cape Town, South Africa
    Article Snippet: .. The presence of the fimbrial adhesion gene (daaC) for diffusely adherent E. coli (DAEC), the anti-aggregation protein transporter gene (aat) for enteroaggregative E. coli (EAggEC) heat-stable (ST ) and heat-labile (LT ) enterotoxin genes for enterotoxigenic E. coli (ETEC), the invasive plasmid antigen (ipa ) gene for enteroinvasive E. coli (EIEC), the bundle-forming pili (bfp ) gene for typical enteropathogenic E. coli (EPEC) and the intimin coding gene (eae ) for EPEC were determined using end-point PCR on an Applied Biosystems™ 2720 Thermal Cycler platform using the QIAGEN PCR-multiplex kit (QIAGEN GmbH, Hilden, Germany) followed by agarose gel detection as previously described [ ], using primers as shown in Table (Inqaba Biotec Laboratory, South Africa). .. The positive control strains that were used for the different diarrheic E. coli pathotypes are shown below (Table ) ([ ]): Similarly, sample TSB enrichment broths from all sources were subjected to nucleic acid extraction using the MagNApure bacterial/fungal DNA extraction kit on a MagNApure LC instrument (Roche Diagnostics, Industriestrasse, Switzerland).

    Article Title: MTHFRC677T and A1298C Genotypes and Haplotypes in Slovenian Couples with Unexplained Infertility Problems and in Embryonic Tissues from Spontaneous Abortions
    Article Snippet: .. Two reactions were performed per sample with the QIAGEN Multiplex PCR Kit (Cat. #206143; Qiagen) by amplifying one allele of each polymorphism (two duplex reactions). ..

    Article Title: Clinical Phenotypes Associated to Engrailed 2 Gene Alterations in a Series of Neuropediatric Patients
    Article Snippet: .. The molecular analysis was carried out by multiplex (QIAGEN Multiplex PCR Kit, catalog number 206145) using the primers described in Table , and the results visualized with QIAxcel System (capillary electrophoresis device, catalog number 9001421, Qiagen, Madrid). .. The results of the genetic study distributed the patients in three groups: 12 patients presented mutations in EN2 gene (EN2-g), n = 12, 20 patients presented mutations in other genes studied in the panel used in our laboratory facilities to detect genetic anomalies in patients with diagnostic of cortical dysplasia (LIS1[PAFAH1B1], PTAFR, PAFAH1B2, PAFAH1B3, FGF8, PAX2, D17S79, D17S1866 , and D17S5 , Table ), but not in EN2 , (OG-g); and 62 patients showing neither alterations in genes of the panel or EN2 ( Table ).

    Article Title: Evidence of Habitat Structuring Aedes albopictus Populations in R?union Island
    Article Snippet: .. Genomic (10 ng) DNA was used for amplification with the QIAGEN multiplex PCR Master Mix kit (ref. 206145) according to the manufacturer's instructions in a final volume of 15 µL. .. One of each pair of primers was fluorescently end-labelled with the fluorochromes NED, VIC, PET or FAM.

    Article Title: All-trans retinoic acid impairs the vasculogenic mimicry formation ability of U87 stem-like cells through promoting differentiation
    Article Snippet: .. PCR was performed in a 50 μ l reaction volume containing 1 μ l complementary DNA, 6X reaction buffer, 0.75 mM MgCl2 , 0.04 mM deoxynucleotide mix, 0.2 pmol/μ l forward primer, 0.2 pmol/μ l reverse primer and 1 Unit Taq polymerase (Qiagen Multiplex PCR kit, cat. no. 206143; Qiagen, Inc., Valencia, CA, USA) for 35 cycles of 30 sec at 94°C, 58°C and 72°C using a Life ECO Thermal Cycler (BYQ6078; Bioer Technology Co., Ltd., Hangzhou, China). .. The primers used for amplification were as follows: Vascular endothelial growth factor (VEGF) sense, 5′-CAGCTACTGCCATCCAATC-3′ and antisense, 5′-CAAATGCTTTCTCCGCTCTG-3′ (313 bp); and GAPDH sense, 5′-TGCCAGTGGTAATACGATT-3′ and antisense, 5′-TAGGAATACTGCCATCACAA-3′ (458 bp).

    Article Title: Identification of Subsets of Enteroaggregative Escherichia coli Associated with Diarrheal Disease among Under 5 Years of Age Children from Rural Gambia
    Article Snippet: .. On group 1 (sat , sepA , pic , sigA , plasmid-encoded enterotoxin [pet ], and astA ), multiplex PCR master mix was achieved using Qiagen kit (Catalogue no. 206143 [Manchester, United Kingdom]) following the manufacturer’s instructions. .. Multiplex PCR assay was performed in a final reaction volume of 25 μL consists of 12.5 μL mastemix (MM), 2.5 μL Q-solution, 6 μL primer (MM), 2.5 μL H2 O, and 1.5 μL DNA template.

    Article Title: Serum Interleukin-18 and Its Gene Haplotypes Profile as Predictors in Patients with Diabetic Nephropathy
    Article Snippet: .. PCR reactions were carried on a thermocycler (Bio-Rad, USA) using 2X master mix (Qiagen, Cat No. 206143 Valencia, USA) according to the manufacturer’s instructions. .. The PCR amplified products were run on 1.5% agarose gel and the bands corresponding to the predicted size were cut and purified using the gel extraction kit (QIA quick columns, Qiagen, Cat No. 28104, Valencia, USA) following the manufacturer’s instructions.

    Article Title: Does host socio-spatial behavior lead to a fine-scale spatial genetic structure in its associated parasites?
    Article Snippet: .. For each sample, seven microsatellites (Hcms25, Hcms27, Hcms33, Hcms36, Hcms40, Hcms22co3 and Hcms8a20 , see [ , ], three multiplexes, see Supplementary Table 1 ) were amplified through polymerase chain reaction (PCR) in a final volume of 15 μL composed of QIAGEN Multiplex PCR kit Mastermix (ref. 206145), 40 nM of each primer, and 2 μL of DNA solution. .. PCR cycles consisted of 15 min of activation (95 °C), followed by 40 cycles of denaturation (30 s, 94 °C), annealing (1 min 30 s at primer-specific annealing temperature, see Supplementary Data 2 ) and extension (1 min, 72 °C).

    Article Title: The SNPforID Assay as a Supplementary Method in Kinship and Trace Analysis
    Article Snippet: .. The application of the Qiagen Multiplex-PCR kit gave more robust and sensitive results compared to the ImmoMixTM (Bioline, Luckenwalde, Germany) and to a self-made PCR mix using Immolase (Bioline; data not shown). .. This necessary procedure is in accordance with studies from other groups such as that of Børsting and colleagues, who also reduced the number of SNPs from 52 to 49 and modified specific PCR conditions [ ].

    Quantitative RT-PCR:

    Article Title: All-trans retinoic acid impairs the vasculogenic mimicry formation ability of U87 stem-like cells through promoting differentiation
    Article Snippet: Paragraph title: Reverse transcription quantitative polymerase chain reaction (RT-qPCR) ... PCR was performed in a 50 μ l reaction volume containing 1 μ l complementary DNA, 6X reaction buffer, 0.75 mM MgCl2 , 0.04 mM deoxynucleotide mix, 0.2 pmol/μ l forward primer, 0.2 pmol/μ l reverse primer and 1 Unit Taq polymerase (Qiagen Multiplex PCR kit, cat. no. 206143; Qiagen, Inc., Valencia, CA, USA) for 35 cycles of 30 sec at 94°C, 58°C and 72°C using a Life ECO Thermal Cycler (BYQ6078; Bioer Technology Co., Ltd., Hangzhou, China).

    Concentration Assay:

    Article Title: Role of Pneumocystis jirovecii in patients with different pulmonary underlying condition using a nested-PCR
    Article Snippet: .. Each PCR reaction contained 5 μL of the extraction product, 12.5 μL QIAGEN Multiplex PCR MasterMix and 0.2 μM concentration of each primer in a total volume of 25 μL (QIAGEN Multiplex PCR kit,Qiagen, Hilden, Germany);5 μL of the first PCR product was used as the DNA template for the second PCR reaction. .. The PCR products were analysed by electrophoresis on 1% agarose gel stained with RedSafe TM Nucleic Acid Staining Solution (iNtRON Biotechnology Inc., Sungnam, Kyungki-Do, Republic of Korea) ( ).

    Article Title: Prevalence and antibiotic susceptibility patterns of enteric bacterial pathogens in human and non-human sources in an urban informal settlement in Cape Town, South Africa
    Article Snippet: All isolates were identified, and their minimum inhibitory concentration (MIC) values determined using VITEK 2 (bioMérieux, USA). .. The presence of the fimbrial adhesion gene (daaC) for diffusely adherent E. coli (DAEC), the anti-aggregation protein transporter gene (aat) for enteroaggregative E. coli (EAggEC) heat-stable (ST ) and heat-labile (LT ) enterotoxin genes for enterotoxigenic E. coli (ETEC), the invasive plasmid antigen (ipa ) gene for enteroinvasive E. coli (EIEC), the bundle-forming pili (bfp ) gene for typical enteropathogenic E. coli (EPEC) and the intimin coding gene (eae ) for EPEC were determined using end-point PCR on an Applied Biosystems™ 2720 Thermal Cycler platform using the QIAGEN PCR-multiplex kit (QIAGEN GmbH, Hilden, Germany) followed by agarose gel detection as previously described [ ], using primers as shown in Table (Inqaba Biotec Laboratory, South Africa).

    Article Title: The nasty neighbour in the striped mouse (Rhabdomys pumilio) steals paternity and elicits aggression
    Article Snippet: .. We used 9 polymorphic microsatellite loci from the house mouse genome (Chr13_1, Chr1_12, Chr1_21, Chr2_3, Chr7_64, D3Mit211, Chr11_81, Chr19_18, Chr5_38), primarily from [ ], and amplified them for all individuals in two multiplexes using the Qiagen PCR-Multiplex-Kit with a final concentration of 0.1/0.2 μM primer for 35 cycles at an annealing temperature of 60°C). ..

    Electrophoresis:

    Article Title: Role of Pneumocystis jirovecii in patients with different pulmonary underlying condition using a nested-PCR
    Article Snippet: Each PCR reaction contained 5 μL of the extraction product, 12.5 μL QIAGEN Multiplex PCR MasterMix and 0.2 μM concentration of each primer in a total volume of 25 μL (QIAGEN Multiplex PCR kit,Qiagen, Hilden, Germany);5 μL of the first PCR product was used as the DNA template for the second PCR reaction. .. The PCR products were analysed by electrophoresis on 1% agarose gel stained with RedSafe TM Nucleic Acid Staining Solution (iNtRON Biotechnology Inc., Sungnam, Kyungki-Do, Republic of Korea) ( ).

    Article Title: Clinical Phenotypes Associated to Engrailed 2 Gene Alterations in a Series of Neuropediatric Patients
    Article Snippet: .. The molecular analysis was carried out by multiplex (QIAGEN Multiplex PCR Kit, catalog number 206145) using the primers described in Table , and the results visualized with QIAxcel System (capillary electrophoresis device, catalog number 9001421, Qiagen, Madrid). .. The results of the genetic study distributed the patients in three groups: 12 patients presented mutations in EN2 gene (EN2-g), n = 12, 20 patients presented mutations in other genes studied in the panel used in our laboratory facilities to detect genetic anomalies in patients with diagnostic of cortical dysplasia (LIS1[PAFAH1B1], PTAFR, PAFAH1B2, PAFAH1B3, FGF8, PAX2, D17S79, D17S1866 , and D17S5 , Table ), but not in EN2 , (OG-g); and 62 patients showing neither alterations in genes of the panel or EN2 ( Table ).

    Article Title: All-trans retinoic acid impairs the vasculogenic mimicry formation ability of U87 stem-like cells through promoting differentiation
    Article Snippet: Reverse transcription quantitative polymerase chain reaction (RT-qPCR) Total RNA was extracted from cells from each group using TRIzol® (Invitrogen Life Technologies SAS) and then verified by electrophoresis using the Sebia Hydrasys 2 agarose gel electrophoresis system (Sebia Inc., Norcross, GA, USA). .. PCR was performed in a 50 μ l reaction volume containing 1 μ l complementary DNA, 6X reaction buffer, 0.75 mM MgCl2 , 0.04 mM deoxynucleotide mix, 0.2 pmol/μ l forward primer, 0.2 pmol/μ l reverse primer and 1 Unit Taq polymerase (Qiagen Multiplex PCR kit, cat. no. 206143; Qiagen, Inc., Valencia, CA, USA) for 35 cycles of 30 sec at 94°C, 58°C and 72°C using a Life ECO Thermal Cycler (BYQ6078; Bioer Technology Co., Ltd., Hangzhou, China).

    Incubation:

    Article Title: Prevalence and antibiotic susceptibility patterns of enteric bacterial pathogens in human and non-human sources in an urban informal settlement in Cape Town, South Africa
    Article Snippet: For P. shigelloides , a loopful of TSB was used to inoculate MacConkey agar with crystal violet (McA) and Cefsulodin – Irgasan - Novobiocin (CIN) agar and incubated at 35 °C for 24 h. Non- lactose fermenting colonies on McA and opaque colonies with a pink center on CIN, were then tested for oxidase activity before confirmatory identification. .. The presence of the fimbrial adhesion gene (daaC) for diffusely adherent E. coli (DAEC), the anti-aggregation protein transporter gene (aat) for enteroaggregative E. coli (EAggEC) heat-stable (ST ) and heat-labile (LT ) enterotoxin genes for enterotoxigenic E. coli (ETEC), the invasive plasmid antigen (ipa ) gene for enteroinvasive E. coli (EIEC), the bundle-forming pili (bfp ) gene for typical enteropathogenic E. coli (EPEC) and the intimin coding gene (eae ) for EPEC were determined using end-point PCR on an Applied Biosystems™ 2720 Thermal Cycler platform using the QIAGEN PCR-multiplex kit (QIAGEN GmbH, Hilden, Germany) followed by agarose gel detection as previously described [ ], using primers as shown in Table (Inqaba Biotec Laboratory, South Africa).

    Article Title: Does host socio-spatial behavior lead to a fine-scale spatial genetic structure in its associated parasites?
    Article Snippet: Following supplier recommendations but adjusting volumes to the small size of samples, the lysis was performed in 100 μL of ACL buffer and 7 μL of proteinase K. Samples were incubated for 1 h at 55 °C under agitation (400 rpm). .. For each sample, seven microsatellites (Hcms25, Hcms27, Hcms33, Hcms36, Hcms40, Hcms22co3 and Hcms8a20 , see [ , ], three multiplexes, see Supplementary Table 1 ) were amplified through polymerase chain reaction (PCR) in a final volume of 15 μL composed of QIAGEN Multiplex PCR kit Mastermix (ref. 206145), 40 nM of each primer, and 2 μL of DNA solution.

    Activity Assay:

    Article Title: Prevalence and antibiotic susceptibility patterns of enteric bacterial pathogens in human and non-human sources in an urban informal settlement in Cape Town, South Africa
    Article Snippet: For P. shigelloides , a loopful of TSB was used to inoculate MacConkey agar with crystal violet (McA) and Cefsulodin – Irgasan - Novobiocin (CIN) agar and incubated at 35 °C for 24 h. Non- lactose fermenting colonies on McA and opaque colonies with a pink center on CIN, were then tested for oxidase activity before confirmatory identification. .. The presence of the fimbrial adhesion gene (daaC) for diffusely adherent E. coli (DAEC), the anti-aggregation protein transporter gene (aat) for enteroaggregative E. coli (EAggEC) heat-stable (ST ) and heat-labile (LT ) enterotoxin genes for enterotoxigenic E. coli (ETEC), the invasive plasmid antigen (ipa ) gene for enteroinvasive E. coli (EIEC), the bundle-forming pili (bfp ) gene for typical enteropathogenic E. coli (EPEC) and the intimin coding gene (eae ) for EPEC were determined using end-point PCR on an Applied Biosystems™ 2720 Thermal Cycler platform using the QIAGEN PCR-multiplex kit (QIAGEN GmbH, Hilden, Germany) followed by agarose gel detection as previously described [ ], using primers as shown in Table (Inqaba Biotec Laboratory, South Africa).

    Expressing:

    Article Title: Landscape features and helminth co-infection shape bank vole immunoheterogeneity, with consequences for Puumala virus epidemiology
    Article Snippet: Paragraph title: Quantification of Tnf-α and Mx2 gene expression ... PCR was performed on anEPgradient S MasterCycler (Qiagen, Venlo, Netherlands), in a final volume of25 μl containing 1 × Qiagen Multiplex PCR Kit,0.2 μ M of each primer and 2 μl of template cDNA diluted1:5.

    Modification:

    Article Title: Germline BRCA1 mutations increase prostate cancer risk
    Article Snippet: Using Qiagen multiplex PCR kit 206145 (Qiagen, Hilden, Germany), the 29 primer pairs were grouped into four multiplexs (3 × 7 multiplexes and 1 × 6 multiplex) and two singleton reactions. .. Multiplex ligation-dependent probe amplification was performed on a subset of 460 samples using a modified protocol of the SALSA MLPA kit P002-C2 (MRC-Holland, Amsterdam, The Netherlands).

    Article Title: The SNPforID Assay as a Supplementary Method in Kinship and Trace Analysis
    Article Snippet: The application of the Qiagen Multiplex-PCR kit gave more robust and sensitive results compared to the ImmoMixTM (Bioline, Luckenwalde, Germany) and to a self-made PCR mix using Immolase (Bioline; data not shown). .. This necessary procedure is in accordance with studies from other groups such as that of Børsting and colleagues, who also reduced the number of SNPs from 52 to 49 and modified specific PCR conditions [ ].

    Agglutination:

    Article Title: Prevalence and antibiotic susceptibility patterns of enteric bacterial pathogens in human and non-human sources in an urban informal settlement in Cape Town, South Africa
    Article Snippet: The presence of the fimbrial adhesion gene (daaC) for diffusely adherent E. coli (DAEC), the anti-aggregation protein transporter gene (aat) for enteroaggregative E. coli (EAggEC) heat-stable (ST ) and heat-labile (LT ) enterotoxin genes for enterotoxigenic E. coli (ETEC), the invasive plasmid antigen (ipa ) gene for enteroinvasive E. coli (EIEC), the bundle-forming pili (bfp ) gene for typical enteropathogenic E. coli (EPEC) and the intimin coding gene (eae ) for EPEC were determined using end-point PCR on an Applied Biosystems™ 2720 Thermal Cycler platform using the QIAGEN PCR-multiplex kit (QIAGEN GmbH, Hilden, Germany) followed by agarose gel detection as previously described [ ], using primers as shown in Table (Inqaba Biotec Laboratory, South Africa). .. Shigella were serotyped using the Wellcolex* colour Shigella Rapid latex agglutination test (Oxoid, Basingstoke, UK), while Salmonella and E. coli (O-typing) [ ] were serotyped at the National Institute for Communicable Disease (NICD), Sandringham, Johannesburg using standard serological methods.

    Ligation:

    Article Title: Germline BRCA1 mutations increase prostate cancer risk
    Article Snippet: Using Qiagen multiplex PCR kit 206145 (Qiagen, Hilden, Germany), the 29 primer pairs were grouped into four multiplexs (3 × 7 multiplexes and 1 × 6 multiplex) and two singleton reactions. .. Multiplex ligation-dependent probe amplification was performed on a subset of 460 samples using a modified protocol of the SALSA MLPA kit P002-C2 (MRC-Holland, Amsterdam, The Netherlands).

    Indirect Immunoperoxidase Assay:

    Article Title: Prevalence and antibiotic susceptibility patterns of enteric bacterial pathogens in human and non-human sources in an urban informal settlement in Cape Town, South Africa
    Article Snippet: .. The presence of the fimbrial adhesion gene (daaC) for diffusely adherent E. coli (DAEC), the anti-aggregation protein transporter gene (aat) for enteroaggregative E. coli (EAggEC) heat-stable (ST ) and heat-labile (LT ) enterotoxin genes for enterotoxigenic E. coli (ETEC), the invasive plasmid antigen (ipa ) gene for enteroinvasive E. coli (EIEC), the bundle-forming pili (bfp ) gene for typical enteropathogenic E. coli (EPEC) and the intimin coding gene (eae ) for EPEC were determined using end-point PCR on an Applied Biosystems™ 2720 Thermal Cycler platform using the QIAGEN PCR-multiplex kit (QIAGEN GmbH, Hilden, Germany) followed by agarose gel detection as previously described [ ], using primers as shown in Table (Inqaba Biotec Laboratory, South Africa). .. The positive control strains that were used for the different diarrheic E. coli pathotypes are shown below (Table ) ([ ]): Similarly, sample TSB enrichment broths from all sources were subjected to nucleic acid extraction using the MagNApure bacterial/fungal DNA extraction kit on a MagNApure LC instrument (Roche Diagnostics, Industriestrasse, Switzerland).

    Generated:

    Article Title: Landscape features and helminth co-infection shape bank vole immunoheterogeneity, with consequences for Puumala virus epidemiology
    Article Snippet: We generated cDNA from 2 μl of extractedRNA (500 ng per reaction), in a 20 μl reaction, with the Improm-IIReverse Transcription System (Promega, Madison, WI, USA), according to the conditionsspecified by the manufacturer for oligo (dT)15 primers. .. PCR was performed on anEPgradient S MasterCycler (Qiagen, Venlo, Netherlands), in a final volume of25 μl containing 1 × Qiagen Multiplex PCR Kit,0.2 μ M of each primer and 2 μl of template cDNA diluted1:5.

    Article Title: The SNPforID Assay as a Supplementary Method in Kinship and Trace Analysis
    Article Snippet: To obtain a satisfactory detection threshold, the remaining 50 PCR products were generated in 2 different multiplex reactions (21- and 29-plex). .. The application of the Qiagen Multiplex-PCR kit gave more robust and sensitive results compared to the ImmoMixTM (Bioline, Luckenwalde, Germany) and to a self-made PCR mix using Immolase (Bioline; data not shown).

    Sequencing:

    Article Title: A High-Speed Congenic Strategy Using First-Wave Male Germ Cells
    Article Snippet: The microsatellite markers were selected out of sequence length polymorphisms between B6 and DBA/2 and B6 and 129 strains (Mekada et al., unpublished data) ( and ). .. PCR execution was performed using the QIAGEN Multiplex PCR kit (#206143; QIAGEN GmbH) and the length polymorphism was detected by agarose gel electrophoresis.

    Article Title: Germline BRCA1 mutations increase prostate cancer risk
    Article Snippet: Using Qiagen multiplex PCR kit 206145 (Qiagen, Hilden, Germany), the 29 primer pairs were grouped into four multiplexs (3 × 7 multiplexes and 1 × 6 multiplex) and two singleton reactions. .. Deleterious mutations were confirmed by Sanger sequencing using a different aliquot from stock DNA of each sample.

    Binding Assay:

    Article Title: A High-Speed Congenic Strategy Using First-Wave Male Germ Cells
    Article Snippet: PCR execution was performed using the QIAGEN Multiplex PCR kit (#206143; QIAGEN GmbH) and the length polymorphism was detected by agarose gel electrophoresis. .. SNP genotyping was carried out using a TaqMan Minor Groove Binding (MGB) assay (Applied Biosystems, Foster City, CA).

    Article Title: Landscape features and helminth co-infection shape bank vole immunoheterogeneity, with consequences for Puumala virus epidemiology
    Article Snippet: We analyzed Tnf-α geneexpression by reverse transcriptase-PCR with the specific primers described in aprevious study ( ):Tnf-a-ex1-F (5′-TTCTGTCTGCTGAACTTCGGA-3′) binding to exon1, Tnf-a-ex3-4-R (5′-GGGTTTGCTACAACGTGG-3′) binding toexons 3 and 4; Actinb-ex3-F (5′-CTTCTACAACGAGCTGCG-3′)binding to exon 3 and Actinb-ex4-R(5′-CCGGAGTCCATCACAAT-3′) binding to exon 4. .. PCR was performed on anEPgradient S MasterCycler (Qiagen, Venlo, Netherlands), in a final volume of25 μl containing 1 × Qiagen Multiplex PCR Kit,0.2 μ M of each primer and 2 μl of template cDNA diluted1:5.

    Cellular Antioxidant Activity Assay:

    Article Title: MTHFRC677T and A1298C Genotypes and Haplotypes in Slovenian Couples with Unexplained Infertility Problems and in Embryonic Tissues from Spontaneous Abortions
    Article Snippet: Two reactions were performed per sample with the QIAGEN Multiplex PCR Kit (Cat. #206143; Qiagen) by amplifying one allele of each polymorphism (two duplex reactions). .. In reaction 1, alleles 677T and 1298A were amplified with MTHFR 677T-F (5′-GAA GGT GTC TGC GGG CGT-3′), MTHFR C677TR (5′-AGC AAC GCT GTG CAA GTT CTG-3′), MTHFR 1298A-F (5′-AGG AGC TGA CCA GTG AGG A-3′) and MTHFR 1298C-R (5′-TTC TCC CTT TGC CAT GTC C-3′).

    Immunofluorescence:

    Article Title: Role of Pneumocystis jirovecii in patients with different pulmonary underlying condition using a nested-PCR
    Article Snippet: Each PCR reaction contained 5 μL of the extraction product, 12.5 μL QIAGEN Multiplex PCR MasterMix and 0.2 μM concentration of each primer in a total volume of 25 μL (QIAGEN Multiplex PCR kit,Qiagen, Hilden, Germany);5 μL of the first PCR product was used as the DNA template for the second PCR reaction. .. During each PCR, a positive control (a BAL fluid sample from a patient with PJP, P. jirovecii positive immunofluorescence assay) and ultra-pure water as the negative control were used.

    Staining:

    Article Title: Role of Pneumocystis jirovecii in patients with different pulmonary underlying condition using a nested-PCR
    Article Snippet: Each PCR reaction contained 5 μL of the extraction product, 12.5 μL QIAGEN Multiplex PCR MasterMix and 0.2 μM concentration of each primer in a total volume of 25 μL (QIAGEN Multiplex PCR kit,Qiagen, Hilden, Germany);5 μL of the first PCR product was used as the DNA template for the second PCR reaction. .. The PCR products were analysed by electrophoresis on 1% agarose gel stained with RedSafe TM Nucleic Acid Staining Solution (iNtRON Biotechnology Inc., Sungnam, Kyungki-Do, Republic of Korea) ( ).

    Article Title: All-trans retinoic acid impairs the vasculogenic mimicry formation ability of U87 stem-like cells through promoting differentiation
    Article Snippet: PCR was performed in a 50 μ l reaction volume containing 1 μ l complementary DNA, 6X reaction buffer, 0.75 mM MgCl2 , 0.04 mM deoxynucleotide mix, 0.2 pmol/μ l forward primer, 0.2 pmol/μ l reverse primer and 1 Unit Taq polymerase (Qiagen Multiplex PCR kit, cat. no. 206143; Qiagen, Inc., Valencia, CA, USA) for 35 cycles of 30 sec at 94°C, 58°C and 72°C using a Life ECO Thermal Cycler (BYQ6078; Bioer Technology Co., Ltd., Hangzhou, China). .. RT-qPCR products were electrophoretically analyzed in 1% agarose and visualized with ethidium bromide staining (Shanghai Haoran Biotechnology Co., Ltd.).

    DNA Extraction:

    Article Title: A High-Speed Congenic Strategy Using First-Wave Male Germ Cells
    Article Snippet: Genotyping for MASP Tail clips about 0.3 cm long were collected for DNA extraction, using the Wizard Genomic DNA Purification Kit (Promega, Madison, WI) and the DNeasy 96 Blood & Tissue Kit (#69582; QIAGEN GmbH, Hilden, Germany) according to the manufacturers' instructions. .. PCR execution was performed using the QIAGEN Multiplex PCR kit (#206143; QIAGEN GmbH) and the length polymorphism was detected by agarose gel electrophoresis.

    Article Title: Prevalence and antibiotic susceptibility patterns of enteric bacterial pathogens in human and non-human sources in an urban informal settlement in Cape Town, South Africa
    Article Snippet: The presence of the fimbrial adhesion gene (daaC) for diffusely adherent E. coli (DAEC), the anti-aggregation protein transporter gene (aat) for enteroaggregative E. coli (EAggEC) heat-stable (ST ) and heat-labile (LT ) enterotoxin genes for enterotoxigenic E. coli (ETEC), the invasive plasmid antigen (ipa ) gene for enteroinvasive E. coli (EIEC), the bundle-forming pili (bfp ) gene for typical enteropathogenic E. coli (EPEC) and the intimin coding gene (eae ) for EPEC were determined using end-point PCR on an Applied Biosystems™ 2720 Thermal Cycler platform using the QIAGEN PCR-multiplex kit (QIAGEN GmbH, Hilden, Germany) followed by agarose gel detection as previously described [ ], using primers as shown in Table (Inqaba Biotec Laboratory, South Africa). .. The positive control strains that were used for the different diarrheic E. coli pathotypes are shown below (Table ) ([ ]): Similarly, sample TSB enrichment broths from all sources were subjected to nucleic acid extraction using the MagNApure bacterial/fungal DNA extraction kit on a MagNApure LC instrument (Roche Diagnostics, Industriestrasse, Switzerland).

    Article Title: MTHFRC677T and A1298C Genotypes and Haplotypes in Slovenian Couples with Unexplained Infertility Problems and in Embryonic Tissues from Spontaneous Abortions
    Article Snippet: Paragraph title: DNA Extraction and Analysis ... Two reactions were performed per sample with the QIAGEN Multiplex PCR Kit (Cat. #206143; Qiagen) by amplifying one allele of each polymorphism (two duplex reactions).

    Fluorescence:

    Article Title: The SNPforID Assay as a Supplementary Method in Kinship and Trace Analysis
    Article Snippet: The application of the Qiagen Multiplex-PCR kit gave more robust and sensitive results compared to the ImmoMixTM (Bioline, Luckenwalde, Germany) and to a self-made PCR mix using Immolase (Bioline; data not shown). .. Fragment analysis conditions were also optimized to achieve signals between 1,000 and 6,000 relative fluorescence units (rfu).

    Mutagenesis:

    Article Title: Germline BRCA1 mutations increase prostate cancer risk
    Article Snippet: Paragraph title: Mutation detection ... Using Qiagen multiplex PCR kit 206145 (Qiagen, Hilden, Germany), the 29 primer pairs were grouped into four multiplexs (3 × 7 multiplexes and 1 × 6 multiplex) and two singleton reactions.

    Isolation:

    Article Title: The nasty neighbour in the striped mouse (Rhabdomys pumilio) steals paternity and elicits aggression
    Article Snippet: Paternity analysis We isolated DNA from mouse tissue using magnetic particle purification (BioSprint 96 DNA Blood Kit, Qiagen). .. We used 9 polymorphic microsatellite loci from the house mouse genome (Chr13_1, Chr1_12, Chr1_21, Chr2_3, Chr7_64, D3Mit211, Chr11_81, Chr19_18, Chr5_38), primarily from [ ], and amplified them for all individuals in two multiplexes using the Qiagen PCR-Multiplex-Kit with a final concentration of 0.1/0.2 μM primer for 35 cycles at an annealing temperature of 60°C).

    Multiplex PCR:

    Article Title: The SNPforID Assay as a Supplementary Method in Kinship and Trace Analysis
    Article Snippet: .. The application of the Qiagen Multiplex-PCR kit gave more robust and sensitive results compared to the ImmoMixTM (Bioline, Luckenwalde, Germany) and to a self-made PCR mix using Immolase (Bioline; data not shown). .. This necessary procedure is in accordance with studies from other groups such as that of Børsting and colleagues, who also reduced the number of SNPs from 52 to 49 and modified specific PCR conditions [ ].

    Multiplex Assay:

    Article Title: A High-Speed Congenic Strategy Using First-Wave Male Germ Cells
    Article Snippet: .. PCR execution was performed using the QIAGEN Multiplex PCR kit (#206143; QIAGEN GmbH) and the length polymorphism was detected by agarose gel electrophoresis. .. Map locations and primer sequences of the microsatellite loci were used according to the Mouse Genome Informatics (MGI) of the Jackson Laboratory, USA, and Mouse Microsatellite Data Base of Japan (MMDBJ).

    Article Title: Role of Pneumocystis jirovecii in patients with different pulmonary underlying condition using a nested-PCR
    Article Snippet: .. Each PCR reaction contained 5 μL of the extraction product, 12.5 μL QIAGEN Multiplex PCR MasterMix and 0.2 μM concentration of each primer in a total volume of 25 μL (QIAGEN Multiplex PCR kit,Qiagen, Hilden, Germany);5 μL of the first PCR product was used as the DNA template for the second PCR reaction. .. The PCR products were analysed by electrophoresis on 1% agarose gel stained with RedSafe TM Nucleic Acid Staining Solution (iNtRON Biotechnology Inc., Sungnam, Kyungki-Do, Republic of Korea) ( ).

    Article Title: Landscape features and helminth co-infection shape bank vole immunoheterogeneity, with consequences for Puumala virus epidemiology
    Article Snippet: .. PCR was performed on anEPgradient S MasterCycler (Qiagen, Venlo, Netherlands), in a final volume of25 μl containing 1 × Qiagen Multiplex PCR Kit,0.2 μ M of each primer and 2 μl of template cDNA diluted1:5. .. Cycling conditions were as follows: initial denaturation at 95 °C for15 min followed by 40 cycles of denaturation at 94 °C for 30 s,annealing at 50 °C for 30 s and elongation at 72 °C for60 s and a final extension phase at 72 °C for 10 min. Sequencesfor both strands were obtained for each sample (Eurofins MWG Synthesis GmbH, Ebersberg,Germany) with the MxUniv2-F and MxUniv2-R primers.

    Article Title: MTHFRC677T and A1298C Genotypes and Haplotypes in Slovenian Couples with Unexplained Infertility Problems and in Embryonic Tissues from Spontaneous Abortions
    Article Snippet: .. Two reactions were performed per sample with the QIAGEN Multiplex PCR Kit (Cat. #206143; Qiagen) by amplifying one allele of each polymorphism (two duplex reactions). ..

    Article Title: Clinical Phenotypes Associated to Engrailed 2 Gene Alterations in a Series of Neuropediatric Patients
    Article Snippet: .. The molecular analysis was carried out by multiplex (QIAGEN Multiplex PCR Kit, catalog number 206145) using the primers described in Table , and the results visualized with QIAxcel System (capillary electrophoresis device, catalog number 9001421, Qiagen, Madrid). .. The results of the genetic study distributed the patients in three groups: 12 patients presented mutations in EN2 gene (EN2-g), n = 12, 20 patients presented mutations in other genes studied in the panel used in our laboratory facilities to detect genetic anomalies in patients with diagnostic of cortical dysplasia (LIS1[PAFAH1B1], PTAFR, PAFAH1B2, PAFAH1B3, FGF8, PAX2, D17S79, D17S1866 , and D17S5 , Table ), but not in EN2 , (OG-g); and 62 patients showing neither alterations in genes of the panel or EN2 ( Table ).

    Article Title: Evidence of Habitat Structuring Aedes albopictus Populations in R?union Island
    Article Snippet: .. Genomic (10 ng) DNA was used for amplification with the QIAGEN multiplex PCR Master Mix kit (ref. 206145) according to the manufacturer's instructions in a final volume of 15 µL. .. One of each pair of primers was fluorescently end-labelled with the fluorochromes NED, VIC, PET or FAM.

    Article Title: All-trans retinoic acid impairs the vasculogenic mimicry formation ability of U87 stem-like cells through promoting differentiation
    Article Snippet: .. PCR was performed in a 50 μ l reaction volume containing 1 μ l complementary DNA, 6X reaction buffer, 0.75 mM MgCl2 , 0.04 mM deoxynucleotide mix, 0.2 pmol/μ l forward primer, 0.2 pmol/μ l reverse primer and 1 Unit Taq polymerase (Qiagen Multiplex PCR kit, cat. no. 206143; Qiagen, Inc., Valencia, CA, USA) for 35 cycles of 30 sec at 94°C, 58°C and 72°C using a Life ECO Thermal Cycler (BYQ6078; Bioer Technology Co., Ltd., Hangzhou, China). .. The primers used for amplification were as follows: Vascular endothelial growth factor (VEGF) sense, 5′-CAGCTACTGCCATCCAATC-3′ and antisense, 5′-CAAATGCTTTCTCCGCTCTG-3′ (313 bp); and GAPDH sense, 5′-TGCCAGTGGTAATACGATT-3′ and antisense, 5′-TAGGAATACTGCCATCACAA-3′ (458 bp).

    Article Title: Identification of Subsets of Enteroaggregative Escherichia coli Associated with Diarrheal Disease among Under 5 Years of Age Children from Rural Gambia
    Article Snippet: .. On group 1 (sat , sepA , pic , sigA , plasmid-encoded enterotoxin [pet ], and astA ), multiplex PCR master mix was achieved using Qiagen kit (Catalogue no. 206143 [Manchester, United Kingdom]) following the manufacturer’s instructions. .. Multiplex PCR assay was performed in a final reaction volume of 25 μL consists of 12.5 μL mastemix (MM), 2.5 μL Q-solution, 6 μL primer (MM), 2.5 μL H2 O, and 1.5 μL DNA template.

    Article Title: Does host socio-spatial behavior lead to a fine-scale spatial genetic structure in its associated parasites?
    Article Snippet: .. For each sample, seven microsatellites (Hcms25, Hcms27, Hcms33, Hcms36, Hcms40, Hcms22co3 and Hcms8a20 , see [ , ], three multiplexes, see Supplementary Table 1 ) were amplified through polymerase chain reaction (PCR) in a final volume of 15 μL composed of QIAGEN Multiplex PCR kit Mastermix (ref. 206145), 40 nM of each primer, and 2 μL of DNA solution. .. PCR cycles consisted of 15 min of activation (95 °C), followed by 40 cycles of denaturation (30 s, 94 °C), annealing (1 min 30 s at primer-specific annealing temperature, see Supplementary Data 2 ) and extension (1 min, 72 °C).

    Article Title: The SNPforID Assay as a Supplementary Method in Kinship and Trace Analysis
    Article Snippet: Figure shows a PAG of different samples inserted into the 2 multiplex reactions. .. The application of the Qiagen Multiplex-PCR kit gave more robust and sensitive results compared to the ImmoMixTM (Bioline, Luckenwalde, Germany) and to a self-made PCR mix using Immolase (Bioline; data not shown).

    Purification:

    Article Title: Clinical Phenotypes Associated to Engrailed 2 Gene Alterations in a Series of Neuropediatric Patients
    Article Snippet: We extracted DNA and RNA from whole blood using QIAamp DNA Mini Kit (catalog number 51306, Qiagen, Madrid) and RNeasy Mini Kit, respectively (catalog number 74106, Qiagen, Madrid) with a robotic workstation for automated purification of DNA and RNA (Qiacube, catalog number 9001292, Qiagen, Madrid). .. The molecular analysis was carried out by multiplex (QIAGEN Multiplex PCR Kit, catalog number 206145) using the primers described in Table , and the results visualized with QIAxcel System (capillary electrophoresis device, catalog number 9001421, Qiagen, Madrid).

    Article Title: Serum Interleukin-18 and Its Gene Haplotypes Profile as Predictors in Patients with Diabetic Nephropathy
    Article Snippet: PCR reactions were carried on a thermocycler (Bio-Rad, USA) using 2X master mix (Qiagen, Cat No. 206143 Valencia, USA) according to the manufacturer’s instructions. .. The PCR amplified products were run on 1.5% agarose gel and the bands corresponding to the predicted size were cut and purified using the gel extraction kit (QIA quick columns, Qiagen, Cat No. 28104, Valencia, USA) following the manufacturer’s instructions.

    Article Title: Does host socio-spatial behavior lead to a fine-scale spatial genetic structure in its associated parasites?
    Article Snippet: Purification and the two washing steps were performed using 150 μL of AB solution and 200 μL of buffer for each washing. .. For each sample, seven microsatellites (Hcms25, Hcms27, Hcms33, Hcms36, Hcms40, Hcms22co3 and Hcms8a20 , see [ , ], three multiplexes, see Supplementary Table 1 ) were amplified through polymerase chain reaction (PCR) in a final volume of 15 μL composed of QIAGEN Multiplex PCR kit Mastermix (ref. 206145), 40 nM of each primer, and 2 μL of DNA solution.

    Article Title: The nasty neighbour in the striped mouse (Rhabdomys pumilio) steals paternity and elicits aggression
    Article Snippet: Paternity analysis We isolated DNA from mouse tissue using magnetic particle purification (BioSprint 96 DNA Blood Kit, Qiagen). .. We used 9 polymorphic microsatellite loci from the house mouse genome (Chr13_1, Chr1_12, Chr1_21, Chr2_3, Chr7_64, D3Mit211, Chr11_81, Chr19_18, Chr5_38), primarily from [ ], and amplified them for all individuals in two multiplexes using the Qiagen PCR-Multiplex-Kit with a final concentration of 0.1/0.2 μM primer for 35 cycles at an annealing temperature of 60°C).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: All-trans retinoic acid impairs the vasculogenic mimicry formation ability of U87 stem-like cells through promoting differentiation
    Article Snippet: RNA was then reverse transcribed using the SuperScript™ III First Strand Synthesis System for RT-PCR (Invitrogen Life Technologies). .. PCR was performed in a 50 μ l reaction volume containing 1 μ l complementary DNA, 6X reaction buffer, 0.75 mM MgCl2 , 0.04 mM deoxynucleotide mix, 0.2 pmol/μ l forward primer, 0.2 pmol/μ l reverse primer and 1 Unit Taq polymerase (Qiagen Multiplex PCR kit, cat. no. 206143; Qiagen, Inc., Valencia, CA, USA) for 35 cycles of 30 sec at 94°C, 58°C and 72°C using a Life ECO Thermal Cycler (BYQ6078; Bioer Technology Co., Ltd., Hangzhou, China).

    Salting Out:

    Article Title: MTHFRC677T and A1298C Genotypes and Haplotypes in Slovenian Couples with Unexplained Infertility Problems and in Embryonic Tissues from Spontaneous Abortions
    Article Snippet: Genomic DNA was extracted from blood leukocytes with a simple salting-out method [ ] . .. Two reactions were performed per sample with the QIAGEN Multiplex PCR Kit (Cat. #206143; Qiagen) by amplifying one allele of each polymorphism (two duplex reactions).

    Nested PCR:

    Article Title: Role of Pneumocystis jirovecii in patients with different pulmonary underlying condition using a nested-PCR
    Article Snippet: The nested-PCR protocol for amplification of mtLSUrRNA in P. jirovecii was performed as previously described by Wakefield et al.[ ]. .. Each PCR reaction contained 5 μL of the extraction product, 12.5 μL QIAGEN Multiplex PCR MasterMix and 0.2 μM concentration of each primer in a total volume of 25 μL (QIAGEN Multiplex PCR kit,Qiagen, Hilden, Germany);5 μL of the first PCR product was used as the DNA template for the second PCR reaction.

    Multiplex Ligation-dependent Probe Amplification:

    Article Title: Germline BRCA1 mutations increase prostate cancer risk
    Article Snippet: Using Qiagen multiplex PCR kit 206145 (Qiagen, Hilden, Germany), the 29 primer pairs were grouped into four multiplexs (3 × 7 multiplexes and 1 × 6 multiplex) and two singleton reactions. .. Multiplex ligation-dependent probe amplification was performed on a subset of 460 samples using a modified protocol of the SALSA MLPA kit P002-C2 (MRC-Holland, Amsterdam, The Netherlands).

    Chloramphenicol Acetyltransferase Assay:

    Article Title: MTHFRC677T and A1298C Genotypes and Haplotypes in Slovenian Couples with Unexplained Infertility Problems and in Embryonic Tissues from Spontaneous Abortions
    Article Snippet: Two reactions were performed per sample with the QIAGEN Multiplex PCR Kit (Cat. #206143; Qiagen) by amplifying one allele of each polymorphism (two duplex reactions). .. In reaction 1, alleles 677T and 1298A were amplified with MTHFR 677T-F (5′-GAA GGT GTC TGC GGG CGT-3′), MTHFR C677TR (5′-AGC AAC GCT GTG CAA GTT CTG-3′), MTHFR 1298A-F (5′-AGG AGC TGA CCA GTG AGG A-3′) and MTHFR 1298C-R (5′-TTC TCC CTT TGC CAT GTC C-3′).

    Plasmid Preparation:

    Article Title: Prevalence and antibiotic susceptibility patterns of enteric bacterial pathogens in human and non-human sources in an urban informal settlement in Cape Town, South Africa
    Article Snippet: .. The presence of the fimbrial adhesion gene (daaC) for diffusely adherent E. coli (DAEC), the anti-aggregation protein transporter gene (aat) for enteroaggregative E. coli (EAggEC) heat-stable (ST ) and heat-labile (LT ) enterotoxin genes for enterotoxigenic E. coli (ETEC), the invasive plasmid antigen (ipa ) gene for enteroinvasive E. coli (EIEC), the bundle-forming pili (bfp ) gene for typical enteropathogenic E. coli (EPEC) and the intimin coding gene (eae ) for EPEC were determined using end-point PCR on an Applied Biosystems™ 2720 Thermal Cycler platform using the QIAGEN PCR-multiplex kit (QIAGEN GmbH, Hilden, Germany) followed by agarose gel detection as previously described [ ], using primers as shown in Table (Inqaba Biotec Laboratory, South Africa). .. The positive control strains that were used for the different diarrheic E. coli pathotypes are shown below (Table ) ([ ]): Similarly, sample TSB enrichment broths from all sources were subjected to nucleic acid extraction using the MagNApure bacterial/fungal DNA extraction kit on a MagNApure LC instrument (Roche Diagnostics, Industriestrasse, Switzerland).

    Article Title: Identification of Subsets of Enteroaggregative Escherichia coli Associated with Diarrheal Disease among Under 5 Years of Age Children from Rural Gambia
    Article Snippet: .. On group 1 (sat , sepA , pic , sigA , plasmid-encoded enterotoxin [pet ], and astA ), multiplex PCR master mix was achieved using Qiagen kit (Catalogue no. 206143 [Manchester, United Kingdom]) following the manufacturer’s instructions. .. Multiplex PCR assay was performed in a final reaction volume of 25 μL consists of 12.5 μL mastemix (MM), 2.5 μL Q-solution, 6 μL primer (MM), 2.5 μL H2 O, and 1.5 μL DNA template.

    Real-time Polymerase Chain Reaction:

    Article Title: Landscape features and helminth co-infection shape bank vole immunoheterogeneity, with consequences for Puumala virus epidemiology
    Article Snippet: PCR was performed on anEPgradient S MasterCycler (Qiagen, Venlo, Netherlands), in a final volume of25 μl containing 1 × Qiagen Multiplex PCR Kit,0.2 μ M of each primer and 2 μl of template cDNA diluted1:5. .. Real-time PCR quantification was performed on a LightCycler 480 (Roche Diagnostics),using the 384-multiwell plate format to quantify Tnf-α and Mx2 gene expression in our samples.

    Article Title: Prevalence and antibiotic susceptibility patterns of enteric bacterial pathogens in human and non-human sources in an urban informal settlement in Cape Town, South Africa
    Article Snippet: E. coli isolates from CHROMagar™STEC were tested for the presence of stx using real-time PCR on a LightCycler®480 II Instrument (Roche Life Sciences, Industriestrasse, Switzerland) using LightCycler® 480 Probes Master mastermix and primers as previously described. (Table ) [ ]. .. The presence of the fimbrial adhesion gene (daaC) for diffusely adherent E. coli (DAEC), the anti-aggregation protein transporter gene (aat) for enteroaggregative E. coli (EAggEC) heat-stable (ST ) and heat-labile (LT ) enterotoxin genes for enterotoxigenic E. coli (ETEC), the invasive plasmid antigen (ipa ) gene for enteroinvasive E. coli (EIEC), the bundle-forming pili (bfp ) gene for typical enteropathogenic E. coli (EPEC) and the intimin coding gene (eae ) for EPEC were determined using end-point PCR on an Applied Biosystems™ 2720 Thermal Cycler platform using the QIAGEN PCR-multiplex kit (QIAGEN GmbH, Hilden, Germany) followed by agarose gel detection as previously described [ ], using primers as shown in Table (Inqaba Biotec Laboratory, South Africa).

    Article Title: All-trans retinoic acid impairs the vasculogenic mimicry formation ability of U87 stem-like cells through promoting differentiation
    Article Snippet: Paragraph title: Reverse transcription quantitative polymerase chain reaction (RT-qPCR) ... PCR was performed in a 50 μ l reaction volume containing 1 μ l complementary DNA, 6X reaction buffer, 0.75 mM MgCl2 , 0.04 mM deoxynucleotide mix, 0.2 pmol/μ l forward primer, 0.2 pmol/μ l reverse primer and 1 Unit Taq polymerase (Qiagen Multiplex PCR kit, cat. no. 206143; Qiagen, Inc., Valencia, CA, USA) for 35 cycles of 30 sec at 94°C, 58°C and 72°C using a Life ECO Thermal Cycler (BYQ6078; Bioer Technology Co., Ltd., Hangzhou, China).

    Negative Control:

    Article Title: Role of Pneumocystis jirovecii in patients with different pulmonary underlying condition using a nested-PCR
    Article Snippet: Each PCR reaction contained 5 μL of the extraction product, 12.5 μL QIAGEN Multiplex PCR MasterMix and 0.2 μM concentration of each primer in a total volume of 25 μL (QIAGEN Multiplex PCR kit,Qiagen, Hilden, Germany);5 μL of the first PCR product was used as the DNA template for the second PCR reaction. .. During each PCR, a positive control (a BAL fluid sample from a patient with PJP, P. jirovecii positive immunofluorescence assay) and ultra-pure water as the negative control were used.

    Positron Emission Tomography:

    Article Title: Evidence of Habitat Structuring Aedes albopictus Populations in R?union Island
    Article Snippet: Genomic (10 ng) DNA was used for amplification with the QIAGEN multiplex PCR Master Mix kit (ref. 206145) according to the manufacturer's instructions in a final volume of 15 µL. .. One of each pair of primers was fluorescently end-labelled with the fluorochromes NED, VIC, PET or FAM.

    Article Title: Identification of Subsets of Enteroaggregative Escherichia coli Associated with Diarrheal Disease among Under 5 Years of Age Children from Rural Gambia
    Article Snippet: .. On group 1 (sat , sepA , pic , sigA , plasmid-encoded enterotoxin [pet ], and astA ), multiplex PCR master mix was achieved using Qiagen kit (Catalogue no. 206143 [Manchester, United Kingdom]) following the manufacturer’s instructions. .. Multiplex PCR assay was performed in a final reaction volume of 25 μL consists of 12.5 μL mastemix (MM), 2.5 μL Q-solution, 6 μL primer (MM), 2.5 μL H2 O, and 1.5 μL DNA template.

    Agarose Gel Electrophoresis:

    Article Title: A High-Speed Congenic Strategy Using First-Wave Male Germ Cells
    Article Snippet: .. PCR execution was performed using the QIAGEN Multiplex PCR kit (#206143; QIAGEN GmbH) and the length polymorphism was detected by agarose gel electrophoresis. .. Map locations and primer sequences of the microsatellite loci were used according to the Mouse Genome Informatics (MGI) of the Jackson Laboratory, USA, and Mouse Microsatellite Data Base of Japan (MMDBJ).

    Article Title: Role of Pneumocystis jirovecii in patients with different pulmonary underlying condition using a nested-PCR
    Article Snippet: Each PCR reaction contained 5 μL of the extraction product, 12.5 μL QIAGEN Multiplex PCR MasterMix and 0.2 μM concentration of each primer in a total volume of 25 μL (QIAGEN Multiplex PCR kit,Qiagen, Hilden, Germany);5 μL of the first PCR product was used as the DNA template for the second PCR reaction. .. The PCR products were analysed by electrophoresis on 1% agarose gel stained with RedSafe TM Nucleic Acid Staining Solution (iNtRON Biotechnology Inc., Sungnam, Kyungki-Do, Republic of Korea) ( ).

    Article Title: Prevalence and antibiotic susceptibility patterns of enteric bacterial pathogens in human and non-human sources in an urban informal settlement in Cape Town, South Africa
    Article Snippet: .. The presence of the fimbrial adhesion gene (daaC) for diffusely adherent E. coli (DAEC), the anti-aggregation protein transporter gene (aat) for enteroaggregative E. coli (EAggEC) heat-stable (ST ) and heat-labile (LT ) enterotoxin genes for enterotoxigenic E. coli (ETEC), the invasive plasmid antigen (ipa ) gene for enteroinvasive E. coli (EIEC), the bundle-forming pili (bfp ) gene for typical enteropathogenic E. coli (EPEC) and the intimin coding gene (eae ) for EPEC were determined using end-point PCR on an Applied Biosystems™ 2720 Thermal Cycler platform using the QIAGEN PCR-multiplex kit (QIAGEN GmbH, Hilden, Germany) followed by agarose gel detection as previously described [ ], using primers as shown in Table (Inqaba Biotec Laboratory, South Africa). .. The positive control strains that were used for the different diarrheic E. coli pathotypes are shown below (Table ) ([ ]): Similarly, sample TSB enrichment broths from all sources were subjected to nucleic acid extraction using the MagNApure bacterial/fungal DNA extraction kit on a MagNApure LC instrument (Roche Diagnostics, Industriestrasse, Switzerland).

    Article Title: All-trans retinoic acid impairs the vasculogenic mimicry formation ability of U87 stem-like cells through promoting differentiation
    Article Snippet: Reverse transcription quantitative polymerase chain reaction (RT-qPCR) Total RNA was extracted from cells from each group using TRIzol® (Invitrogen Life Technologies SAS) and then verified by electrophoresis using the Sebia Hydrasys 2 agarose gel electrophoresis system (Sebia Inc., Norcross, GA, USA). .. PCR was performed in a 50 μ l reaction volume containing 1 μ l complementary DNA, 6X reaction buffer, 0.75 mM MgCl2 , 0.04 mM deoxynucleotide mix, 0.2 pmol/μ l forward primer, 0.2 pmol/μ l reverse primer and 1 Unit Taq polymerase (Qiagen Multiplex PCR kit, cat. no. 206143; Qiagen, Inc., Valencia, CA, USA) for 35 cycles of 30 sec at 94°C, 58°C and 72°C using a Life ECO Thermal Cycler (BYQ6078; Bioer Technology Co., Ltd., Hangzhou, China).

    Article Title: Serum Interleukin-18 and Its Gene Haplotypes Profile as Predictors in Patients with Diabetic Nephropathy
    Article Snippet: PCR reactions were carried on a thermocycler (Bio-Rad, USA) using 2X master mix (Qiagen, Cat No. 206143 Valencia, USA) according to the manufacturer’s instructions. .. The PCR amplified products were run on 1.5% agarose gel and the bands corresponding to the predicted size were cut and purified using the gel extraction kit (QIA quick columns, Qiagen, Cat No. 28104, Valencia, USA) following the manufacturer’s instructions.

    Size-exclusion Chromatography:

    Article Title: All-trans retinoic acid impairs the vasculogenic mimicry formation ability of U87 stem-like cells through promoting differentiation
    Article Snippet: .. PCR was performed in a 50 μ l reaction volume containing 1 μ l complementary DNA, 6X reaction buffer, 0.75 mM MgCl2 , 0.04 mM deoxynucleotide mix, 0.2 pmol/μ l forward primer, 0.2 pmol/μ l reverse primer and 1 Unit Taq polymerase (Qiagen Multiplex PCR kit, cat. no. 206143; Qiagen, Inc., Valencia, CA, USA) for 35 cycles of 30 sec at 94°C, 58°C and 72°C using a Life ECO Thermal Cycler (BYQ6078; Bioer Technology Co., Ltd., Hangzhou, China). .. The primers used for amplification were as follows: Vascular endothelial growth factor (VEGF) sense, 5′-CAGCTACTGCCATCCAATC-3′ and antisense, 5′-CAAATGCTTTCTCCGCTCTG-3′ (313 bp); and GAPDH sense, 5′-TGCCAGTGGTAATACGATT-3′ and antisense, 5′-TAGGAATACTGCCATCACAA-3′ (458 bp).

    Produced:

    Article Title: Role of Pneumocystis jirovecii in patients with different pulmonary underlying condition using a nested-PCR
    Article Snippet: The external primers pAZ102-E and pAZ102-H to mtLSUrRNA P. jirovecii gene amplification were used in the first amplification round which produced a 346 bp amplicon, being included in the same reaction as an internal control 16s rRNA gene amplification primers (U1 and U2), which produced a 996 bp product [ ]. .. Each PCR reaction contained 5 μL of the extraction product, 12.5 μL QIAGEN Multiplex PCR MasterMix and 0.2 μM concentration of each primer in a total volume of 25 μL (QIAGEN Multiplex PCR kit,Qiagen, Hilden, Germany);5 μL of the first PCR product was used as the DNA template for the second PCR reaction.

    Activation Assay:

    Article Title: Does host socio-spatial behavior lead to a fine-scale spatial genetic structure in its associated parasites?
    Article Snippet: For each sample, seven microsatellites (Hcms25, Hcms27, Hcms33, Hcms36, Hcms40, Hcms22co3 and Hcms8a20 , see [ , ], three multiplexes, see Supplementary Table 1 ) were amplified through polymerase chain reaction (PCR) in a final volume of 15 μL composed of QIAGEN Multiplex PCR kit Mastermix (ref. 206145), 40 nM of each primer, and 2 μL of DNA solution. .. PCR cycles consisted of 15 min of activation (95 °C), followed by 40 cycles of denaturation (30 s, 94 °C), annealing (1 min 30 s at primer-specific annealing temperature, see Supplementary Data 2 ) and extension (1 min, 72 °C).

    DNA Purification:

    Article Title: A High-Speed Congenic Strategy Using First-Wave Male Germ Cells
    Article Snippet: Genotyping for MASP Tail clips about 0.3 cm long were collected for DNA extraction, using the Wizard Genomic DNA Purification Kit (Promega, Madison, WI) and the DNeasy 96 Blood & Tissue Kit (#69582; QIAGEN GmbH, Hilden, Germany) according to the manufacturers' instructions. .. PCR execution was performed using the QIAGEN Multiplex PCR kit (#206143; QIAGEN GmbH) and the length polymorphism was detected by agarose gel electrophoresis.

    Lysis:

    Article Title: Does host socio-spatial behavior lead to a fine-scale spatial genetic structure in its associated parasites?
    Article Snippet: Following supplier recommendations but adjusting volumes to the small size of samples, the lysis was performed in 100 μL of ACL buffer and 7 μL of proteinase K. Samples were incubated for 1 h at 55 °C under agitation (400 rpm). .. For each sample, seven microsatellites (Hcms25, Hcms27, Hcms33, Hcms36, Hcms40, Hcms22co3 and Hcms8a20 , see [ , ], three multiplexes, see Supplementary Table 1 ) were amplified through polymerase chain reaction (PCR) in a final volume of 15 μL composed of QIAGEN Multiplex PCR kit Mastermix (ref. 206145), 40 nM of each primer, and 2 μL of DNA solution.

    CTG Assay:

    Article Title: MTHFRC677T and A1298C Genotypes and Haplotypes in Slovenian Couples with Unexplained Infertility Problems and in Embryonic Tissues from Spontaneous Abortions
    Article Snippet: Two reactions were performed per sample with the QIAGEN Multiplex PCR Kit (Cat. #206143; Qiagen) by amplifying one allele of each polymorphism (two duplex reactions). .. In reaction 1, alleles 677T and 1298A were amplified with MTHFR 677T-F (5′-GAA GGT GTC TGC GGG CGT-3′), MTHFR C677TR (5′-AGC AAC GCT GTG CAA GTT CTG-3′), MTHFR 1298A-F (5′-AGG AGC TGA CCA GTG AGG A-3′) and MTHFR 1298C-R (5′-TTC TCC CTT TGC CAT GTC C-3′).

    Gel Extraction:

    Article Title: Serum Interleukin-18 and Its Gene Haplotypes Profile as Predictors in Patients with Diabetic Nephropathy
    Article Snippet: PCR reactions were carried on a thermocycler (Bio-Rad, USA) using 2X master mix (Qiagen, Cat No. 206143 Valencia, USA) according to the manufacturer’s instructions. .. The PCR amplified products were run on 1.5% agarose gel and the bands corresponding to the predicted size were cut and purified using the gel extraction kit (QIA quick columns, Qiagen, Cat No. 28104, Valencia, USA) following the manufacturer’s instructions.

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    Qiagen qiagen multiplex pcr kit
    Qiagen Multiplex Pcr Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qiagen multiplex pcr kit/product/Qiagen
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    qiagen multiplex pcr kit - by Bioz Stars, 2020-01
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