quantitect rev transcription kit  (Qiagen)


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    Name:
    QuantiTect Rev Transcription Kit
    Description:
    For fast cDNA synthesis enabling sensitive real time two step RT PCR for gene expression analysis Kit contents Qiagen QuantiTect Reverse Transcription Kit 10 x 20L rxns RNA Template Sample Two step cDNA Production Genomic DNA Digestion Reaction Type For Fast cDNA Synthesis Enabling Sensitive Real time Two step RT PCR for Gene Expression Analysis Includes 10 x 20L rxns 100L 7x gDNA Wipeout Buffer 10L Quantiscript Reverse Transcriptase 200L 5x Quantiscript RT Buffer 50L RT Primer Mix 1 9mL RNase free Water Benefits cDNA synthesis and gDNA removal in only 20 minutes High cDNA yields even from low abundance transcripts cDNA synthesis from 5 and 3 regions of transcripts No need to design RNA specific primers or prob
    Catalog Number:
    205310
    Price:
    101
    Category:
    QuantiTect Reverse Transcription Kit
    Buy from Supplier


    Structured Review

    Qiagen quantitect rev transcription kit
    QuantiTect Rev Transcription Kit
    For fast cDNA synthesis enabling sensitive real time two step RT PCR for gene expression analysis Kit contents Qiagen QuantiTect Reverse Transcription Kit 10 x 20L rxns RNA Template Sample Two step cDNA Production Genomic DNA Digestion Reaction Type For Fast cDNA Synthesis Enabling Sensitive Real time Two step RT PCR for Gene Expression Analysis Includes 10 x 20L rxns 100L 7x gDNA Wipeout Buffer 10L Quantiscript Reverse Transcriptase 200L 5x Quantiscript RT Buffer 50L RT Primer Mix 1 9mL RNase free Water Benefits cDNA synthesis and gDNA removal in only 20 minutes High cDNA yields even from low abundance transcripts cDNA synthesis from 5 and 3 regions of transcripts No need to design RNA specific primers or prob
    https://www.bioz.com/result/quantitect rev transcription kit/product/Qiagen
    Average 90 stars, based on 227 article reviews
    Price from $9.99 to $1999.99
    quantitect rev transcription kit - by Bioz Stars, 2020-01
    90/100 stars

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    Related Articles

    Amplification:

    Article Title: Modelling TFE renal cell carcinoma in mice reveals a critical role of WNT signaling
    Article Snippet: Reverse transcription was performed using QuantiTect Rev Transcription Kit (Qiagen). .. The parameters of real-time PCR amplification were defined according to Roche recommendations.

    Article Title: l-Lactate Production from Biodiesel-Derived Crude Glycerol by Metabolically Engineered Enterococcus faecalis: Cytotoxic Evaluation of Biodiesel Waste and Development of a Glycerol-Inducible Gene Expression System
    Article Snippet: Total RNA was purified using the RNeasy Protect Bacteria minikit (Qiagen, Hilden, Germany) with the RNase-Free DNase Set (Qiagen), and cDNA was synthesized using the QuantiTect Rev Transcription Kit (Qiagen). .. The specific amplicons for the 16S rRNA, glycerol dehydrogenase ( gldA ), DHA kinase ( dhaK ), glycerol kinase ( glpK ), glycerol-3P oxidase ( glpO ), alkyl hydroperoxide reductase ( ahpC ), thioredoxin peroxidase ( tpx ), and NADH peroxidase ( npr ) genes were amplified by PCR using cDNA as a template, the primers shown in , and KOD-Plus DNA polymerase (Toyobo).

    Article Title: Placental Underperfusion in a Rat Model of Intrauterine Growth Restriction Induced by a Reduced Plasma Volume Expansion
    Article Snippet: Single-strand cDNA was synthesized according to the procedure in the QuantiTect Rev Transcription Kit (Qiagen Toronto, ON, Canada). .. The targeted and referenced genes were amplified in duplicate in the same run using the Mx3000P Q-PCR System (Stratagene, CA, USA).

    Article Title: Tanacetumpolycephalum (L.) Schultz-Bip. Induces Mitochondrial-Mediated Apoptosis and Inhibits Migration and Invasion in MCF7 Cells
    Article Snippet: The reverse transcription reaction for the isolated RNA was carried out using the QuantiTect Rev Transcription Kit (Qiagen). .. The transcribed cDNA (1 μL) was applied for the RT-PCR amplification, with specific primers for Bax (sense, 5′-TTCTGACGGCAACTTCAACTG-3′ and antisense, 5′-AGCACTCCCGCCACAAAGA-3′), Bcl-2 (sense, 5′-AGACATCAGCATGGCTCAAA-3′, and antisense, 5′-GATTCACTGGGTAAGACTAAAGGA-3′) and β-actin (sense, 5′-CGGGAAATCGTGCGTGAC-3′, and antisense, 5′-GCCTAGAAGCATTTGCGGTG-3′) genes. β-actin gene served as the loading control in this study.

    Article Title: Expression of Dentin Sialophosphoprotein in Non-mineralized Tissues
    Article Snippet: The RNA (1 µg/per sample) was reverse transcribed into first-strand cDNA using the QuantiTect Rev Transcription Kit (Qiagen; Germantown, MD). .. The PCR amplification of the DSPP cDNA was then performed using a forward primer, 5′-TTGTTGAAAACTCTGTGGCTGTGC-3′, and a reverse primer, 5′-GGCATCGTTGAAAATGCGGAGGAA-3′.

    Article Title: An "ex vivo model" contributing to the diagnosis and evaluation of new drugs in cystic fibrosis
    Article Snippet: RNA concentration and purity was determined with a Nano- Drop ND-1000 spectrophotometer; reverse transcription was carried out on 1 μg of total RNA resuspended in DEPC-treated nano-pure water using a QuantiTect Rev Transcription Kit (Qiagen, CA) using the protocol supplied by the manufacturer. .. Amplification was carried out with a LightCycler 480 System for real-time PCR (Roche) with a two-step PCR protocol (preincubation for 10 min at + 95° C followed by 45 cycles of amplification: 95°C for 10 sec, 60°C for 25 sec, 72°C for 1 sec). mRNA quantification results were normalised using glyceraldehyde 3-phosphate dehydrogenase (GAPDH ) gene (Roche, ID.

    Article Title: Spatial Engineering of Osteochondral Tissue Constructs Through Microfluidically Directed Differentiation of Mesenchymal Stem Cells
    Article Snippet: Reverse transcription of the RNA into cDNA was achieved using a QuantiTect Rev Transcription Kit (QIAGEN). .. Real-Time PCR amplification was performed (StepOnePlus™; Applied Biosystems) in the presence of SYBR Green/ROX master mix and primers for target osteochondral lineage markers ( ).

    Article Title: Induction of Interferon Pathways Mediates In Vivo Resistance to Oncolytic Adenovirus
    Article Snippet: .. Total cellular RNA of SKOV3.ip1 xenografts was reverse-transcribed into cDNA using Qiagen QuantiTect Reverse Transcription Kit (205311; Hilden, Germany), which included genomic DNA wipeout, and concentrations of the cDNAs were balanced to 100 ng/µl using NanoDrop 2000 Spectrophotometer (Thermo Scientific, Waltham, WA). cDNA samples were amplified using LightCycler480 SYBR Green I Master mix (Roche, Mannheim, Germany) and specific primers to analyze mRNA levels for human MxA (5′-ACCTACAGCTGGCTCCTGAA-3′ and 5′-CGGCTAACGGATAAGCAGAG-3′), IFI27 (5′-ACCTCATCAGCAGTGACCAGT-3′ and 5′-ACATCATCTTGGCTGCTATGG-3′), IFI16 (5-ACTGAGTACAACAAAGCCATTTGA-3 and 5′-TTGTGACATTGTCCTGTCCCCAC-3′). ..

    Article Title: Genetic Interactions Underlying the Biosynthesis and Inhibition of ?-Diketones in Wheat and Their Impact on Glaucousness and Cuticle Permeability
    Article Snippet: After RNA integrity evaluation by agarose gel electrophoresis and quantification using Nanodrop ND-1000 (Thermo Scientific), 1 µg of total RNA was used for cDNA synthesis in a 20-µl reaction using the QuantiTect Rev Transcription Kit (Qiagen). .. The comparative ΔΔCT method was used to evaluate the relative quantities of each amplified product using TaRPII36 as an internal reference in the same run.

    Synthesized:

    Article Title: l-Lactate Production from Biodiesel-Derived Crude Glycerol by Metabolically Engineered Enterococcus faecalis: Cytotoxic Evaluation of Biodiesel Waste and Development of a Glycerol-Inducible Gene Expression System
    Article Snippet: .. Total RNA was purified using the RNeasy Protect Bacteria minikit (Qiagen, Hilden, Germany) with the RNase-Free DNase Set (Qiagen), and cDNA was synthesized using the QuantiTect Rev Transcription Kit (Qiagen). .. The specific amplicons for the 16S rRNA, glycerol dehydrogenase ( gldA ), DHA kinase ( dhaK ), glycerol kinase ( glpK ), glycerol-3P oxidase ( glpO ), alkyl hydroperoxide reductase ( ahpC ), thioredoxin peroxidase ( tpx ), and NADH peroxidase ( npr ) genes were amplified by PCR using cDNA as a template, the primers shown in , and KOD-Plus DNA polymerase (Toyobo).

    Article Title: Placental Underperfusion in a Rat Model of Intrauterine Growth Restriction Induced by a Reduced Plasma Volume Expansion
    Article Snippet: .. Single-strand cDNA was synthesized according to the procedure in the QuantiTect Rev Transcription Kit (Qiagen Toronto, ON, Canada). .. Q-PCR reactions were carried out using the Biotool SYBR® Green (Biotool, Cedarlane, Montreal, QC, Canada) and specific primers.

    Quantitative RT-PCR:

    Article Title: Longitudinal field studies reveal early infection and persistence of influenza A virus in piglets despite the presence of maternally derived antibodies
    Article Snippet: Paragraph title: Reverse transcription real-time RT-PCR ... The QuantiTect Rev transcription kit (QIAGEN) was used with the primers and probes from a previous study [ ] with a few primer adjustments, as listed in Table .

    Article Title: Heat in Wheat: Exploit Reverse Genetic Techniques to Discover New Alleles Within the Triticum durum sHsp26 Family
    Article Snippet: Cham1 was extracted with RNeasy Plant Mini Kit (Qiagen, Hilden, Germany),cDNA was generated with QuantiTect Rev.Transcription Kit (Qiagen). .. 20 ng/μL cDNA were used for Real Time (RT) qPCR analysis by using the ABI PRISM® 7000 Sequence Detection System (Applied Biosystems, Foster City, USA).

    Article Title: Spatial Engineering of Osteochondral Tissue Constructs Through Microfluidically Directed Differentiation of Mesenchymal Stem Cells
    Article Snippet: mRNA expression Quantitative reverse transcription polymerase chain reaction (RT-qPCR) was used to quantify gene expression within the constructs in a region specific manner. .. Reverse transcription of the RNA into cDNA was achieved using a QuantiTect Rev Transcription Kit (QIAGEN).

    Real-time Polymerase Chain Reaction:

    Article Title: Modelling TFE renal cell carcinoma in mice reveals a critical role of WNT signaling
    Article Snippet: Paragraph title: Quantitative real-time PCR ... Reverse transcription was performed using QuantiTect Rev Transcription Kit (Qiagen).

    Article Title: Lgt: a Family of Cytotoxic Glucosyltransferases Produced by Legionella pneumophila
    Article Snippet: For reverse transcription (RT), a QuantiTect Rev transcription kit (Qiagen) was utilized as suggested by the manufacturer. .. Real-time PCR was performed with a QuantiTect SYBR green PCR kit (Qiagen) on an Mx3000P cycler controlled by MxPro software (Stratagene, Amsterdam, Netherlands).

    Article Title: Placental Underperfusion in a Rat Model of Intrauterine Growth Restriction Induced by a Reduced Plasma Volume Expansion
    Article Snippet: Paragraph title: Real-Time Quantitative PCR (qPCR) ... Single-strand cDNA was synthesized according to the procedure in the QuantiTect Rev Transcription Kit (Qiagen Toronto, ON, Canada).

    Article Title: Role of NH2-terminal fragment of Dentin Sialophosphoprotein (DSPP) in dentinogenesis
    Article Snippet: Quantitative real-time PCR (qPCR) was performed to determine the DSP/DSP-PG mRNA expression level in the incisors of the eight founder mice in the wild type genetic background as previously described ( , ). .. The RNA (1 ug/per sample) was reverse-transcribed into cDNA using the QuantiTect Rev Transcription Kit (Qiagen; Germantown, MD).

    Article Title: Expression of Dentin Sialophosphoprotein in Non-mineralized Tissues
    Article Snippet: Paragraph title: Isolation of RNA, RT-PCR, and Real-time PCR ... The RNA (1 µg/per sample) was reverse transcribed into first-strand cDNA using the QuantiTect Rev Transcription Kit (Qiagen; Germantown, MD).

    Article Title: An "ex vivo model" contributing to the diagnosis and evaluation of new drugs in cystic fibrosis
    Article Snippet: Paragraph title: Real-time PCR for quantitative analysis of CFTR mRNA ... RNA concentration and purity was determined with a Nano- Drop ND-1000 spectrophotometer; reverse transcription was carried out on 1 μg of total RNA resuspended in DEPC-treated nano-pure water using a QuantiTect Rev Transcription Kit (Qiagen, CA) using the protocol supplied by the manufacturer.

    Article Title: Spatial Engineering of Osteochondral Tissue Constructs Through Microfluidically Directed Differentiation of Mesenchymal Stem Cells
    Article Snippet: Reverse transcription of the RNA into cDNA was achieved using a QuantiTect Rev Transcription Kit (QIAGEN). .. Real-Time PCR amplification was performed (StepOnePlus™; Applied Biosystems) in the presence of SYBR Green/ROX master mix and primers for target osteochondral lineage markers ( ).

    Article Title: Genetic Interactions Underlying the Biosynthesis and Inhibition of ?-Diketones in Wheat and Their Impact on Glaucousness and Cuticle Permeability
    Article Snippet: The maximal cut-off value was set at E-20. qPCR primers were designed using the Primer3 program ( http://frodo.wi.mit.edu/ ), with the PCR product size ranging from 80 to 200 bp ( ). .. After RNA integrity evaluation by agarose gel electrophoresis and quantification using Nanodrop ND-1000 (Thermo Scientific), 1 µg of total RNA was used for cDNA synthesis in a 20-µl reaction using the QuantiTect Rev Transcription Kit (Qiagen).

    Microarray:

    Article Title: Transparent Testa16 Plays Multiple Roles in Plant Development and Is Involved in Lipid Synthesis and Embryo Development in Canola 1 Plays Multiple Roles in Plant Development and Is Involved in Lipid Synthesis and Embryo Development in Canola 1 [W] Plays Multiple Roles in Plant Development and Is Involved in Lipid Synthesis and Embryo Development in Canola 1 [W] [OA]
    Article Snippet: DNase-treated RNA was then reverse transcribed using the QuantiTect Rev Transcription kit (Qiagen). .. Quantitative reverse transcription-PCR was performed to confirm microarray results according to the method reported previously by .

    Two Tailed Test:

    Article Title: Induction of Interferon Pathways Mediates In Vivo Resistance to Oncolytic Adenovirus
    Article Snippet: Total cellular RNA of SKOV3.ip1 xenografts was reverse-transcribed into cDNA using Qiagen QuantiTect Reverse Transcription Kit (205311; Hilden, Germany), which included genomic DNA wipeout, and concentrations of the cDNAs were balanced to 100 ng/µl using NanoDrop 2000 Spectrophotometer (Thermo Scientific, Waltham, WA). cDNA samples were amplified using LightCycler480 SYBR Green I Master mix (Roche, Mannheim, Germany) and specific primers to analyze mRNA levels for human MxA (5′-ACCTACAGCTGGCTCCTGAA-3′ and 5′-CGGCTAACGGATAAGCAGAG-3′), IFI27 (5′-ACCTCATCAGCAGTGACCAGT-3′ and 5′-ACATCATCTTGGCTGCTATGG-3′), IFI16 (5-ACTGAGTACAACAAAGCCATTTGA-3 and 5′-TTGTGACATTGTCCTGTCCCCAC-3′). .. The expression levels of each gene were expressed as fold increase in five treated tumors compared to five nontreated tumors, and analyzed using two-tailed Student's t -test (SPSS 15.0; SPSS).

    In Silico:

    Article Title: Heat in Wheat: Exploit Reverse Genetic Techniques to Discover New Alleles Within the Triticum durum sHsp26 Family
    Article Snippet: Paragraph title: In vitro and in silico TdHsp26 transcription profiling ... Cham1 was extracted with RNeasy Plant Mini Kit (Qiagen, Hilden, Germany),cDNA was generated with QuantiTect Rev.Transcription Kit (Qiagen).

    Expressing:

    Article Title: Modelling TFE renal cell carcinoma in mice reveals a critical role of WNT signaling
    Article Snippet: Reverse transcription was performed using QuantiTect Rev Transcription Kit (Qiagen). .. To quantify gene expression, Gapdh mRNA expression was used as an internal reference.

    Article Title: Placental Underperfusion in a Rat Model of Intrauterine Growth Restriction Induced by a Reduced Plasma Volume Expansion
    Article Snippet: Single-strand cDNA was synthesized according to the procedure in the QuantiTect Rev Transcription Kit (Qiagen Toronto, ON, Canada). .. The mRNA levels were normalized to 18S expression levels.

    Article Title: Role of NH2-terminal fragment of Dentin Sialophosphoprotein (DSPP) in dentinogenesis
    Article Snippet: Paragraph title: Expression levels of the DSP transgenes in teeth ... The RNA (1 ug/per sample) was reverse-transcribed into cDNA using the QuantiTect Rev Transcription Kit (Qiagen; Germantown, MD).

    Article Title: Formation of 53BP1 foci and ATM activation under oxidative stress is facilitated by RNA:DNA hybrids and loss of ATM-53BP1 expression promotes photoreceptor cell survival in mice
    Article Snippet: Paragraph title: Primers and PCR for ATM expression in neural retina ... RNA was quantified using Nanodrop ND-100 Spectrophotometer (Manufactured by NanoDrop Technologies), normalized and reverse transcribed to cDNA using QuantiTect Reverse Transcription Kit (Qiagen,#205311).

    Article Title: Tanacetumpolycephalum (L.) Schultz-Bip. Induces Mitochondrial-Mediated Apoptosis and Inhibits Migration and Invasion in MCF7 Cells
    Article Snippet: Paragraph title: 3.10. Analysis of mRNA Expression of Bax and Bcl-2 by RT-PCR ... The reverse transcription reaction for the isolated RNA was carried out using the QuantiTect Rev Transcription Kit (Qiagen).

    Article Title: Spatial Engineering of Osteochondral Tissue Constructs Through Microfluidically Directed Differentiation of Mesenchymal Stem Cells
    Article Snippet: Paragraph title: mRNA expression ... Reverse transcription of the RNA into cDNA was achieved using a QuantiTect Rev Transcription Kit (QIAGEN).

    Article Title: Induction of Interferon Pathways Mediates In Vivo Resistance to Oncolytic Adenovirus
    Article Snippet: Total cellular RNA of SKOV3.ip1 xenografts was reverse-transcribed into cDNA using Qiagen QuantiTect Reverse Transcription Kit (205311; Hilden, Germany), which included genomic DNA wipeout, and concentrations of the cDNAs were balanced to 100 ng/µl using NanoDrop 2000 Spectrophotometer (Thermo Scientific, Waltham, WA). cDNA samples were amplified using LightCycler480 SYBR Green I Master mix (Roche, Mannheim, Germany) and specific primers to analyze mRNA levels for human MxA (5′-ACCTACAGCTGGCTCCTGAA-3′ and 5′-CGGCTAACGGATAAGCAGAG-3′), IFI27 (5′-ACCTCATCAGCAGTGACCAGT-3′ and 5′-ACATCATCTTGGCTGCTATGG-3′), IFI16 (5-ACTGAGTACAACAAAGCCATTTGA-3 and 5′-TTGTGACATTGTCCTGTCCCCAC-3′). .. The expression levels of each gene were expressed as fold increase in five treated tumors compared to five nontreated tumors, and analyzed using two-tailed Student's t -test (SPSS 15.0; SPSS).

    Western Blot:

    Article Title: Formation of 53BP1 foci and ATM activation under oxidative stress is facilitated by RNA:DNA hybrids and loss of ATM-53BP1 expression promotes photoreceptor cell survival in mice
    Article Snippet: Primers and PCR for ATM expression in neural retina The neural retina of two mice for each time point (at postnatal days 10, 20, 60 and 270) was microdissected; 1 eye each was used for western blot and one eye was used to prepare total RNA. .. RNA was quantified using Nanodrop ND-100 Spectrophotometer (Manufactured by NanoDrop Technologies), normalized and reverse transcribed to cDNA using QuantiTect Reverse Transcription Kit (Qiagen,#205311).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Lgt: a Family of Cytotoxic Glucosyltransferases Produced by Legionella pneumophila
    Article Snippet: Paragraph title: RT-PCR. ... For reverse transcription (RT), a QuantiTect Rev transcription kit (Qiagen) was utilized as suggested by the manufacturer.

    Article Title: l-Lactate Production from Biodiesel-Derived Crude Glycerol by Metabolically Engineered Enterococcus faecalis: Cytotoxic Evaluation of Biodiesel Waste and Development of a Glycerol-Inducible Gene Expression System
    Article Snippet: Paragraph title: Reverse transcription (RT)-PCR. ... Total RNA was purified using the RNeasy Protect Bacteria minikit (Qiagen, Hilden, Germany) with the RNase-Free DNase Set (Qiagen), and cDNA was synthesized using the QuantiTect Rev Transcription Kit (Qiagen).

    Article Title: Tanacetumpolycephalum (L.) Schultz-Bip. Induces Mitochondrial-Mediated Apoptosis and Inhibits Migration and Invasion in MCF7 Cells
    Article Snippet: Paragraph title: 3.10. Analysis of mRNA Expression of Bax and Bcl-2 by RT-PCR ... The reverse transcription reaction for the isolated RNA was carried out using the QuantiTect Rev Transcription Kit (Qiagen).

    Article Title: Expression of Dentin Sialophosphoprotein in Non-mineralized Tissues
    Article Snippet: Paragraph title: Isolation of RNA, RT-PCR, and Real-time PCR ... The RNA (1 µg/per sample) was reverse transcribed into first-strand cDNA using the QuantiTect Rev Transcription Kit (Qiagen; Germantown, MD).

    Article Title: Spatial Engineering of Osteochondral Tissue Constructs Through Microfluidically Directed Differentiation of Mesenchymal Stem Cells
    Article Snippet: mRNA expression Quantitative reverse transcription polymerase chain reaction (RT-qPCR) was used to quantify gene expression within the constructs in a region specific manner. .. Reverse transcription of the RNA into cDNA was achieved using a QuantiTect Rev Transcription Kit (QIAGEN).

    Generated:

    Article Title: Heat in Wheat: Exploit Reverse Genetic Techniques to Discover New Alleles Within the Triticum durum sHsp26 Family
    Article Snippet: .. Cham1 was extracted with RNeasy Plant Mini Kit (Qiagen, Hilden, Germany),cDNA was generated with QuantiTect Rev.Transcription Kit (Qiagen). .. 20 ng/μL cDNA were used for Real Time (RT) qPCR analysis by using the ABI PRISM® 7000 Sequence Detection System (Applied Biosystems, Foster City, USA).

    Sequencing:

    Article Title: Placental Underperfusion in a Rat Model of Intrauterine Growth Restriction Induced by a Reduced Plasma Volume Expansion
    Article Snippet: PCR primers specific for genes of interest ( ) were designed with PRIMER3 ( www.genome.wi.mit.edu/cgi-bin/primer/primer3_www.cgi ) based on sequence data from the National Center for Biotechnology Information and obtained from Life Technologies (Burlington, ON, Canada). .. Single-strand cDNA was synthesized according to the procedure in the QuantiTect Rev Transcription Kit (Qiagen Toronto, ON, Canada).

    Article Title: Formation of 53BP1 foci and ATM activation under oxidative stress is facilitated by RNA:DNA hybrids and loss of ATM-53BP1 expression promotes photoreceptor cell survival in mice
    Article Snippet: RNA was quantified using Nanodrop ND-100 Spectrophotometer (Manufactured by NanoDrop Technologies), normalized and reverse transcribed to cDNA using QuantiTect Reverse Transcription Kit (Qiagen,#205311). .. Primers were designed using the Primer3Plus online tool, on the selected exons of ATM cDNA sequence (from the ENSEMBL database).

    Article Title: Expression of Dentin Sialophosphoprotein in Non-mineralized Tissues
    Article Snippet: The RNA (1 µg/per sample) was reverse transcribed into first-strand cDNA using the QuantiTect Rev Transcription Kit (Qiagen; Germantown, MD). .. The sequence of the forward primer came from exon 3, whereas that of reverse primer was from exon 4 of the mouse Dspp .

    Article Title: Heat in Wheat: Exploit Reverse Genetic Techniques to Discover New Alleles Within the Triticum durum sHsp26 Family
    Article Snippet: Cham1 was extracted with RNeasy Plant Mini Kit (Qiagen, Hilden, Germany),cDNA was generated with QuantiTect Rev.Transcription Kit (Qiagen). .. 20 ng/μL cDNA were used for Real Time (RT) qPCR analysis by using the ABI PRISM® 7000 Sequence Detection System (Applied Biosystems, Foster City, USA).

    Fluorescence:

    Article Title: Lgt: a Family of Cytotoxic Glucosyltransferases Produced by Legionella pneumophila
    Article Snippet: For reverse transcription (RT), a QuantiTect Rev transcription kit (Qiagen) was utilized as suggested by the manufacturer. .. The following equation was used to calculate induction in the RT-PCR experiments: ratio = , where Δ CT = CT (sample 1) − CT (sample 2) ( ) and CT means the cycle threshold at which the fluorescence level reaches the cutoff value, determined by the computer program.

    Article Title: Spatial Engineering of Osteochondral Tissue Constructs Through Microfluidically Directed Differentiation of Mesenchymal Stem Cells
    Article Snippet: Reverse transcription of the RNA into cDNA was achieved using a QuantiTect Rev Transcription Kit (QIAGEN). .. Regulation of the target genes over day 0 controls was determined by processing the raw fluorescence data using LinRegPCR (v12.11; www.hartfaalcentrum.nl ) with glyceraldehyde-3-phosphate dehydrogenase (GAPDH ) and β-actin (ACTB ) serving as the endogenous controls through geometric averaging.

    Isolation:

    Article Title: Modelling TFE renal cell carcinoma in mice reveals a critical role of WNT signaling
    Article Snippet: Quantitative real-time PCR Total RNA was isolated from frozen samples lysed in Trizol (Life Technologies) using a TissueLyser (Qiagen) and following the recommended manufacturer’s protocol. .. Reverse transcription was performed using QuantiTect Rev Transcription Kit (Qiagen).

    Article Title: Lgt: a Family of Cytotoxic Glucosyltransferases Produced by Legionella pneumophila
    Article Snippet: Total RNA from L. pneumophila Philadelphia-1 cells, grown in proteose-peptone broth or in A. castellanii for certain periods, was isolated using an RNeasy mini kit with RNAprotect bacterial reagent (Qiagen, Hilden, Germany). .. For reverse transcription (RT), a QuantiTect Rev transcription kit (Qiagen) was utilized as suggested by the manufacturer.

    Article Title: Placental Underperfusion in a Rat Model of Intrauterine Growth Restriction Induced by a Reduced Plasma Volume Expansion
    Article Snippet: Real-Time Quantitative PCR (qPCR) Total RNA was isolated from frozen placental tissue using Qiazol reagent according to the manufacturer's instructions RNeasy Lipid Tissue Mini Kit (Qiagen, Toronto, ON, Canada). .. Single-strand cDNA was synthesized according to the procedure in the QuantiTect Rev Transcription Kit (Qiagen Toronto, ON, Canada).

    Article Title: Tanacetumpolycephalum (L.) Schultz-Bip. Induces Mitochondrial-Mediated Apoptosis and Inhibits Migration and Invasion in MCF7 Cells
    Article Snippet: .. The reverse transcription reaction for the isolated RNA was carried out using the QuantiTect Rev Transcription Kit (Qiagen). .. The transcribed cDNA (1 μL) was applied for the RT-PCR amplification, with specific primers for Bax (sense, 5′-TTCTGACGGCAACTTCAACTG-3′ and antisense, 5′-AGCACTCCCGCCACAAAGA-3′), Bcl-2 (sense, 5′-AGACATCAGCATGGCTCAAA-3′, and antisense, 5′-GATTCACTGGGTAAGACTAAAGGA-3′) and β-actin (sense, 5′-CGGGAAATCGTGCGTGAC-3′, and antisense, 5′-GCCTAGAAGCATTTGCGGTG-3′) genes. β-actin gene served as the loading control in this study.

    Article Title: Expression of Dentin Sialophosphoprotein in Non-mineralized Tissues
    Article Snippet: Paragraph title: Isolation of RNA, RT-PCR, and Real-time PCR ... The RNA (1 µg/per sample) was reverse transcribed into first-strand cDNA using the QuantiTect Rev Transcription Kit (Qiagen; Germantown, MD).

    Article Title: An "ex vivo model" contributing to the diagnosis and evaluation of new drugs in cystic fibrosis
    Article Snippet: Real-time PCR for quantitative analysis of CFTR mRNA Total RNA was isolated from nasal epithelial cells using TRIzol reagent (Invitrogen, Italy) as previously described . .. RNA concentration and purity was determined with a Nano- Drop ND-1000 spectrophotometer; reverse transcription was carried out on 1 μg of total RNA resuspended in DEPC-treated nano-pure water using a QuantiTect Rev Transcription Kit (Qiagen, CA) using the protocol supplied by the manufacturer.

    Article Title: Genetic Interactions Underlying the Biosynthesis and Inhibition of ?-Diketones in Wheat and Their Impact on Glaucousness and Cuticle Permeability
    Article Snippet: Total RNA was isolated from flag leaf sheaths using Trizol® reagent (Invitrogen), following the manufacturer’s instructions. .. After RNA integrity evaluation by agarose gel electrophoresis and quantification using Nanodrop ND-1000 (Thermo Scientific), 1 µg of total RNA was used for cDNA synthesis in a 20-µl reaction using the QuantiTect Rev Transcription Kit (Qiagen).

    Size-exclusion Chromatography:

    Article Title: An "ex vivo model" contributing to the diagnosis and evaluation of new drugs in cystic fibrosis
    Article Snippet: RNA concentration and purity was determined with a Nano- Drop ND-1000 spectrophotometer; reverse transcription was carried out on 1 μg of total RNA resuspended in DEPC-treated nano-pure water using a QuantiTect Rev Transcription Kit (Qiagen, CA) using the protocol supplied by the manufacturer. .. Amplification was carried out with a LightCycler 480 System for real-time PCR (Roche) with a two-step PCR protocol (preincubation for 10 min at + 95° C followed by 45 cycles of amplification: 95°C for 10 sec, 60°C for 25 sec, 72°C for 1 sec). mRNA quantification results were normalised using glyceraldehyde 3-phosphate dehydrogenase (GAPDH ) gene (Roche, ID.

    Mouse Assay:

    Article Title: Role of NH2-terminal fragment of Dentin Sialophosphoprotein (DSPP) in dentinogenesis
    Article Snippet: Quantitative real-time PCR (qPCR) was performed to determine the DSP/DSP-PG mRNA expression level in the incisors of the eight founder mice in the wild type genetic background as previously described ( , ). .. The RNA (1 ug/per sample) was reverse-transcribed into cDNA using the QuantiTect Rev Transcription Kit (Qiagen; Germantown, MD).

    Article Title: Formation of 53BP1 foci and ATM activation under oxidative stress is facilitated by RNA:DNA hybrids and loss of ATM-53BP1 expression promotes photoreceptor cell survival in mice
    Article Snippet: Primers and PCR for ATM expression in neural retina The neural retina of two mice for each time point (at postnatal days 10, 20, 60 and 270) was microdissected; 1 eye each was used for western blot and one eye was used to prepare total RNA. .. RNA was quantified using Nanodrop ND-100 Spectrophotometer (Manufactured by NanoDrop Technologies), normalized and reverse transcribed to cDNA using QuantiTect Reverse Transcription Kit (Qiagen,#205311).

    Article Title: Expression of Dentin Sialophosphoprotein in Non-mineralized Tissues
    Article Snippet: Total RNA was extracted from the tissues of 1-month-old mice (C57BL/6J) with the RNeasy mini kit (Qiagen; Germantown, MD) according to the manufacturer’s protocol. .. The RNA (1 µg/per sample) was reverse transcribed into first-strand cDNA using the QuantiTect Rev Transcription Kit (Qiagen; Germantown, MD).

    Polymerase Chain Reaction:

    Article Title: Lgt: a Family of Cytotoxic Glucosyltransferases Produced by Legionella pneumophila
    Article Snippet: For reverse transcription (RT), a QuantiTect Rev transcription kit (Qiagen) was utilized as suggested by the manufacturer. .. Real-time PCR was performed with a QuantiTect SYBR green PCR kit (Qiagen) on an Mx3000P cycler controlled by MxPro software (Stratagene, Amsterdam, Netherlands).

    Article Title: Longitudinal field studies reveal early infection and persistence of influenza A virus in piglets despite the presence of maternally derived antibodies
    Article Snippet: The QuantiTect Rev transcription kit (QIAGEN) was used with the primers and probes from a previous study [ ] with a few primer adjustments, as listed in Table . .. The QuantiTect Rev transcription kit (QIAGEN) was used with the primers and probes from a previous study [ ] with a few primer adjustments, as listed in Table .

    Article Title: l-Lactate Production from Biodiesel-Derived Crude Glycerol by Metabolically Engineered Enterococcus faecalis: Cytotoxic Evaluation of Biodiesel Waste and Development of a Glycerol-Inducible Gene Expression System
    Article Snippet: Total RNA was purified using the RNeasy Protect Bacteria minikit (Qiagen, Hilden, Germany) with the RNase-Free DNase Set (Qiagen), and cDNA was synthesized using the QuantiTect Rev Transcription Kit (Qiagen). .. The specific amplicons for the 16S rRNA, glycerol dehydrogenase ( gldA ), DHA kinase ( dhaK ), glycerol kinase ( glpK ), glycerol-3P oxidase ( glpO ), alkyl hydroperoxide reductase ( ahpC ), thioredoxin peroxidase ( tpx ), and NADH peroxidase ( npr ) genes were amplified by PCR using cDNA as a template, the primers shown in , and KOD-Plus DNA polymerase (Toyobo).

    Article Title: Placental Underperfusion in a Rat Model of Intrauterine Growth Restriction Induced by a Reduced Plasma Volume Expansion
    Article Snippet: PCR primers specific for genes of interest ( ) were designed with PRIMER3 ( www.genome.wi.mit.edu/cgi-bin/primer/primer3_www.cgi ) based on sequence data from the National Center for Biotechnology Information and obtained from Life Technologies (Burlington, ON, Canada). .. Single-strand cDNA was synthesized according to the procedure in the QuantiTect Rev Transcription Kit (Qiagen Toronto, ON, Canada).

    Article Title: Role of NH2-terminal fragment of Dentin Sialophosphoprotein (DSPP) in dentinogenesis
    Article Snippet: The RNA (1 ug/per sample) was reverse-transcribed into cDNA using the QuantiTect Rev Transcription Kit (Qiagen; Germantown, MD). .. The real-time PCR reactions were performed using PCR conditions as we previously reported ( ).

    Article Title: Formation of 53BP1 foci and ATM activation under oxidative stress is facilitated by RNA:DNA hybrids and loss of ATM-53BP1 expression promotes photoreceptor cell survival in mice
    Article Snippet: Paragraph title: Primers and PCR for ATM expression in neural retina ... RNA was quantified using Nanodrop ND-100 Spectrophotometer (Manufactured by NanoDrop Technologies), normalized and reverse transcribed to cDNA using QuantiTect Reverse Transcription Kit (Qiagen,#205311).

    Article Title: Expression of Dentin Sialophosphoprotein in Non-mineralized Tissues
    Article Snippet: The RNA (1 µg/per sample) was reverse transcribed into first-strand cDNA using the QuantiTect Rev Transcription Kit (Qiagen; Germantown, MD). .. The PCR amplification of the DSPP cDNA was then performed using a forward primer, 5′-TTGTTGAAAACTCTGTGGCTGTGC-3′, and a reverse primer, 5′-GGCATCGTTGAAAATGCGGAGGAA-3′.

    Article Title: An "ex vivo model" contributing to the diagnosis and evaluation of new drugs in cystic fibrosis
    Article Snippet: RNA concentration and purity was determined with a Nano- Drop ND-1000 spectrophotometer; reverse transcription was carried out on 1 μg of total RNA resuspended in DEPC-treated nano-pure water using a QuantiTect Rev Transcription Kit (Qiagen, CA) using the protocol supplied by the manufacturer. .. Amplification was carried out with a LightCycler 480 System for real-time PCR (Roche) with a two-step PCR protocol (preincubation for 10 min at + 95° C followed by 45 cycles of amplification: 95°C for 10 sec, 60°C for 25 sec, 72°C for 1 sec). mRNA quantification results were normalised using glyceraldehyde 3-phosphate dehydrogenase (GAPDH ) gene (Roche, ID.

    Article Title: Genetic Interactions Underlying the Biosynthesis and Inhibition of ?-Diketones in Wheat and Their Impact on Glaucousness and Cuticle Permeability
    Article Snippet: The maximal cut-off value was set at E-20. qPCR primers were designed using the Primer3 program ( http://frodo.wi.mit.edu/ ), with the PCR product size ranging from 80 to 200 bp ( ). .. After RNA integrity evaluation by agarose gel electrophoresis and quantification using Nanodrop ND-1000 (Thermo Scientific), 1 µg of total RNA was used for cDNA synthesis in a 20-µl reaction using the QuantiTect Rev Transcription Kit (Qiagen).

    Construct:

    Article Title: Spatial Engineering of Osteochondral Tissue Constructs Through Microfluidically Directed Differentiation of Mesenchymal Stem Cells
    Article Snippet: mRNA expression Quantitative reverse transcription polymerase chain reaction (RT-qPCR) was used to quantify gene expression within the constructs in a region specific manner. .. Reverse transcription of the RNA into cDNA was achieved using a QuantiTect Rev Transcription Kit (QIAGEN).

    Purification:

    Article Title: l-Lactate Production from Biodiesel-Derived Crude Glycerol by Metabolically Engineered Enterococcus faecalis: Cytotoxic Evaluation of Biodiesel Waste and Development of a Glycerol-Inducible Gene Expression System
    Article Snippet: .. Total RNA was purified using the RNeasy Protect Bacteria minikit (Qiagen, Hilden, Germany) with the RNase-Free DNase Set (Qiagen), and cDNA was synthesized using the QuantiTect Rev Transcription Kit (Qiagen). .. The specific amplicons for the 16S rRNA, glycerol dehydrogenase ( gldA ), DHA kinase ( dhaK ), glycerol kinase ( glpK ), glycerol-3P oxidase ( glpO ), alkyl hydroperoxide reductase ( ahpC ), thioredoxin peroxidase ( tpx ), and NADH peroxidase ( npr ) genes were amplified by PCR using cDNA as a template, the primers shown in , and KOD-Plus DNA polymerase (Toyobo).

    Software:

    Article Title: Lgt: a Family of Cytotoxic Glucosyltransferases Produced by Legionella pneumophila
    Article Snippet: For reverse transcription (RT), a QuantiTect Rev transcription kit (Qiagen) was utilized as suggested by the manufacturer. .. Real-time PCR was performed with a QuantiTect SYBR green PCR kit (Qiagen) on an Mx3000P cycler controlled by MxPro software (Stratagene, Amsterdam, Netherlands).

    SYBR Green Assay:

    Article Title: Modelling TFE renal cell carcinoma in mice reveals a critical role of WNT signaling
    Article Snippet: Reverse transcription was performed using QuantiTect Rev Transcription Kit (Qiagen). .. Finally, real-time PCR was performed using SYBR Green (Roche Diagnostics) and performing the reaction in the LightCycler System 2.0 (Roche Applied Science).

    Article Title: Lgt: a Family of Cytotoxic Glucosyltransferases Produced by Legionella pneumophila
    Article Snippet: For reverse transcription (RT), a QuantiTect Rev transcription kit (Qiagen) was utilized as suggested by the manufacturer. .. Real-time PCR was performed with a QuantiTect SYBR green PCR kit (Qiagen) on an Mx3000P cycler controlled by MxPro software (Stratagene, Amsterdam, Netherlands).

    Article Title: Placental Underperfusion in a Rat Model of Intrauterine Growth Restriction Induced by a Reduced Plasma Volume Expansion
    Article Snippet: Single-strand cDNA was synthesized according to the procedure in the QuantiTect Rev Transcription Kit (Qiagen Toronto, ON, Canada). .. Q-PCR reactions were carried out using the Biotool SYBR® Green (Biotool, Cedarlane, Montreal, QC, Canada) and specific primers.

    Article Title: Induction of Interferon Pathways Mediates In Vivo Resistance to Oncolytic Adenovirus
    Article Snippet: .. Total cellular RNA of SKOV3.ip1 xenografts was reverse-transcribed into cDNA using Qiagen QuantiTect Reverse Transcription Kit (205311; Hilden, Germany), which included genomic DNA wipeout, and concentrations of the cDNAs were balanced to 100 ng/µl using NanoDrop 2000 Spectrophotometer (Thermo Scientific, Waltham, WA). cDNA samples were amplified using LightCycler480 SYBR Green I Master mix (Roche, Mannheim, Germany) and specific primers to analyze mRNA levels for human MxA (5′-ACCTACAGCTGGCTCCTGAA-3′ and 5′-CGGCTAACGGATAAGCAGAG-3′), IFI27 (5′-ACCTCATCAGCAGTGACCAGT-3′ and 5′-ACATCATCTTGGCTGCTATGG-3′), IFI16 (5-ACTGAGTACAACAAAGCCATTTGA-3 and 5′-TTGTGACATTGTCCTGTCCCCAC-3′). ..

    Article Title: Genetic Interactions Underlying the Biosynthesis and Inhibition of ?-Diketones in Wheat and Their Impact on Glaucousness and Cuticle Permeability
    Article Snippet: After RNA integrity evaluation by agarose gel electrophoresis and quantification using Nanodrop ND-1000 (Thermo Scientific), 1 µg of total RNA was used for cDNA synthesis in a 20-µl reaction using the QuantiTect Rev Transcription Kit (Qiagen). .. After dilution, ∼5 ng of cDNA was used as template for qPCR in a volume of 20 µl. qPCR was performed in 96-well plates with an ABI 7900HT High-Throughput Real-Time Thermocycler (Life Tech) using the iTaq™ Fast SYBR® Green Supermix with ROX (Bio-Rad).

    Multiplex Assay:

    Article Title: Longitudinal field studies reveal early infection and persistence of influenza A virus in piglets despite the presence of maternally derived antibodies
    Article Snippet: All positive individual samples with a ct value < 31 were then retested using a multiplex RT-rtPCR assay to determine the influenza A subtype. .. The QuantiTect Rev transcription kit (QIAGEN) was used with the primers and probes from a previous study [ ] with a few primer adjustments, as listed in Table .

    Agarose Gel Electrophoresis:

    Article Title: Genetic Interactions Underlying the Biosynthesis and Inhibition of ?-Diketones in Wheat and Their Impact on Glaucousness and Cuticle Permeability
    Article Snippet: .. After RNA integrity evaluation by agarose gel electrophoresis and quantification using Nanodrop ND-1000 (Thermo Scientific), 1 µg of total RNA was used for cDNA synthesis in a 20-µl reaction using the QuantiTect Rev Transcription Kit (Qiagen). .. After dilution, ∼5 ng of cDNA was used as template for qPCR in a volume of 20 µl. qPCR was performed in 96-well plates with an ABI 7900HT High-Throughput Real-Time Thermocycler (Life Tech) using the iTaq™ Fast SYBR® Green Supermix with ROX (Bio-Rad).

    In Vitro:

    Article Title: Heat in Wheat: Exploit Reverse Genetic Techniques to Discover New Alleles Within the Triticum durum sHsp26 Family
    Article Snippet: Paragraph title: In vitro and in silico TdHsp26 transcription profiling ... Cham1 was extracted with RNeasy Plant Mini Kit (Qiagen, Hilden, Germany),cDNA was generated with QuantiTect Rev.Transcription Kit (Qiagen).

    Spectrophotometry:

    Article Title: Formation of 53BP1 foci and ATM activation under oxidative stress is facilitated by RNA:DNA hybrids and loss of ATM-53BP1 expression promotes photoreceptor cell survival in mice
    Article Snippet: .. RNA was quantified using Nanodrop ND-100 Spectrophotometer (Manufactured by NanoDrop Technologies), normalized and reverse transcribed to cDNA using QuantiTect Reverse Transcription Kit (Qiagen,#205311). .. Primers were designed using the Primer3Plus online tool, on the selected exons of ATM cDNA sequence (from the ENSEMBL database).

    Article Title: An "ex vivo model" contributing to the diagnosis and evaluation of new drugs in cystic fibrosis
    Article Snippet: .. RNA concentration and purity was determined with a Nano- Drop ND-1000 spectrophotometer; reverse transcription was carried out on 1 μg of total RNA resuspended in DEPC-treated nano-pure water using a QuantiTect Rev Transcription Kit (Qiagen, CA) using the protocol supplied by the manufacturer. .. To evaluate levels of CFTR transcript in nasal epithelial cells, relative quantification by real-time PCR was performed in duplicates using LightCycler 480 Probes Master containing CFTR primers (Roche, Italy) and a TaqMan CFTR probe (ID.

    Article Title: Induction of Interferon Pathways Mediates In Vivo Resistance to Oncolytic Adenovirus
    Article Snippet: .. Total cellular RNA of SKOV3.ip1 xenografts was reverse-transcribed into cDNA using Qiagen QuantiTect Reverse Transcription Kit (205311; Hilden, Germany), which included genomic DNA wipeout, and concentrations of the cDNAs were balanced to 100 ng/µl using NanoDrop 2000 Spectrophotometer (Thermo Scientific, Waltham, WA). cDNA samples were amplified using LightCycler480 SYBR Green I Master mix (Roche, Mannheim, Germany) and specific primers to analyze mRNA levels for human MxA (5′-ACCTACAGCTGGCTCCTGAA-3′ and 5′-CGGCTAACGGATAAGCAGAG-3′), IFI27 (5′-ACCTCATCAGCAGTGACCAGT-3′ and 5′-ACATCATCTTGGCTGCTATGG-3′), IFI16 (5-ACTGAGTACAACAAAGCCATTTGA-3 and 5′-TTGTGACATTGTCCTGTCCCCAC-3′). ..

    Concentration Assay:

    Article Title: An "ex vivo model" contributing to the diagnosis and evaluation of new drugs in cystic fibrosis
    Article Snippet: .. RNA concentration and purity was determined with a Nano- Drop ND-1000 spectrophotometer; reverse transcription was carried out on 1 μg of total RNA resuspended in DEPC-treated nano-pure water using a QuantiTect Rev Transcription Kit (Qiagen, CA) using the protocol supplied by the manufacturer. .. To evaluate levels of CFTR transcript in nasal epithelial cells, relative quantification by real-time PCR was performed in duplicates using LightCycler 480 Probes Master containing CFTR primers (Roche, Italy) and a TaqMan CFTR probe (ID.

    High Throughput Screening Assay:

    Article Title: Genetic Interactions Underlying the Biosynthesis and Inhibition of ?-Diketones in Wheat and Their Impact on Glaucousness and Cuticle Permeability
    Article Snippet: After RNA integrity evaluation by agarose gel electrophoresis and quantification using Nanodrop ND-1000 (Thermo Scientific), 1 µg of total RNA was used for cDNA synthesis in a 20-µl reaction using the QuantiTect Rev Transcription Kit (Qiagen). .. After dilution, ∼5 ng of cDNA was used as template for qPCR in a volume of 20 µl. qPCR was performed in 96-well plates with an ABI 7900HT High-Throughput Real-Time Thermocycler (Life Tech) using the iTaq™ Fast SYBR® Green Supermix with ROX (Bio-Rad).

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    Qiagen quantitect rev transcription kit
    Quantitect Rev Transcription Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 227 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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