quantitect probe pcr kit  (Qiagen)


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    Name:
    QuantiTect Probe PCR Kit
    Description:
    For real time PCR and two step RT PCR using sequence specific probes Kit contents Qiagen QuantiTect Probe PCR Kit 200 x 50L rxns DNA cDNA RNA Sample PCR and Two step RT PCR Reaction Sequence specific Probes Ideal for Real time Quantification of DNA cDNA or RNA Targets For Real time PCR and Two step RT PCR Using Sequence specific Probes Includes 3 x 1 7mL 2x QuantiTect Probe PCR Master Mix 2 x 2mL RNase free Water Benefits Highly sensitive detection of low copy targets Accurate quantification over several logs of template Use of any sequence specific probe on any real time cycler Available with or without uracil N glycosylase UNG No need to optimize reaction and cycling cond
    Catalog Number:
    204343
    Price:
    460
    Category:
    QuantiTect Probe PCR Kits
    Buy from Supplier


    Structured Review

    Qiagen quantitect probe pcr kit
    QuantiTect Probe PCR Kit
    For real time PCR and two step RT PCR using sequence specific probes Kit contents Qiagen QuantiTect Probe PCR Kit 200 x 50L rxns DNA cDNA RNA Sample PCR and Two step RT PCR Reaction Sequence specific Probes Ideal for Real time Quantification of DNA cDNA or RNA Targets For Real time PCR and Two step RT PCR Using Sequence specific Probes Includes 3 x 1 7mL 2x QuantiTect Probe PCR Master Mix 2 x 2mL RNase free Water Benefits Highly sensitive detection of low copy targets Accurate quantification over several logs of template Use of any sequence specific probe on any real time cycler Available with or without uracil N glycosylase UNG No need to optimize reaction and cycling cond
    https://www.bioz.com/result/quantitect probe pcr kit/product/Qiagen
    Average 90 stars, based on 135 article reviews
    Price from $9.99 to $1999.99
    quantitect probe pcr kit - by Bioz Stars, 2020-01
    90/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Natural Single-Nucleotide Variations in the HIV-1 Genomic SA1prox Region Can Alter Viral Replication Ability by Regulating Vif Expression Levels
    Article Snippet: For qRT-PCR analysis, samples used for semiquantitative RT-PCR analysis were subjected to qRT-PCR analysis by using the QuantiTect Probe PCR kit (Qiagen GmbH), using the primer pairs for qRT-PCR analysis of viral transcripts described above. .. Briefly, Proviral clones and an LTR-driven luciferase reporter clone, 5RLTR-Luc , were cotransfected into HEK 293T cells with Lipofectamine 2000 (Life Technologies Corporation).

    Amplification:

    Article Title: Recombinant human erythropoietin increases survival and reduces neuronal apoptosis in a murine model of cerebral malaria
    Article Snippet: All real time-PCR reactions were carried out on a MX-3000p (Stratagene, USA) and run for 50 cycles using the QuantiTect Probe PCR Kit (Qiagen, USA) according to the manufacturers protocol and the following protein specific kits: β-actin, cat. no. 241014, caspase 3, cat. no. 241124, no. 241054, caspase 1, cat. no. 241151, TNF, cat. no. 241034, IFN-γ, cat. no. 241036, IL-1β, cat. and LT: cat no. 241174. .. Cycle-threshold (Ct) values were defined as the PCR cycle at which amplified product was first detected.

    Article Title: Kaposi's Sarcoma-Associated Herpesvirus Inhibits Expression and Function of Endothelial Cell Major Histocompatibility Complex Class II via Suppressor of Cytokine Signaling 3
    Article Snippet: RNA was extracted from EC using TRIzol (Invitrogen), and quantitative reverse transcriptase PCR (qRT-PCR) was conducted either in one step with a QuantiTect probe PCR kit (Qiagen) ( SOCS3 and CIITA ) or in two steps with conversion to cDNA, followed by qPCR, using TaqMan reagents (Applied Biosystems). .. Samples were amplified using the 7900 HT real-time PCR machine and analyzed using the software package SDS 2.2 (Applied Biosystems).

    Article Title: Membrane-Free Stem Cell Components Inhibit Interleukin-1α-Stimulated Inflammation and Cartilage Degradation In Vitro and In Vivo: A Rat Model of Osteoarthritis
    Article Snippet: Obtained total RNA was quantified using a NanoDrop spectrophotometer and were reverse-transcribed at 42 °C into cDNA for 30 min using QuantiTect Probe PCR kit (Qiagen, GmbH, Hilden, Germany). .. The cDNA was successively amplified by PCR using the PCR Master Mix (Promega, Madison, WI, USA) according to the manufacturer’s instructions in a Thermal Cycler Dice® Real-Time PCR system (TaKaRa Bio, Kyoto, Japan).

    Article Title: Analysis of MicroRNA Expression in Newborns with Differential Birth Weight Using Newborn Screening Cards
    Article Snippet: Real Time Quantitative PCR Sequences of specific forward primers are listed on , a universal reverse primer (URP) and universal probe library probe #21 (UPL-21) were the same for all miRNAs, according to Czimmerer et al. method [ ]. qPCR reactions were carried out on a Roche’s LightCycler 2.0 (Roche Diagnostics, Mannheim, Germany) using QuantiTect Probe PCR Kit (Qiagen, Hilden, Germany) in a final volume of 20 µL containing: 5 µL of cDNA dilution, 0.1 µL of 100 µM specific reverse primer, 0.1 µL of 100 µM URP, 0.2 µL of probe UPL #21 (Roche) and 4.6 µL of nuclease free water; PCR reactions were performed by duplicate. .. There was no detection signal in non-template control (NTC) or omitting reverse transcriptase enzyme reaction (negative control) after 50 cycles of amplification.

    Article Title: A Copine family member, Cpne8, is a candidate quantitative trait gene for prion disease incubation time in mouse
    Article Snippet: MGB probes labelled with either Vic or Fam were purchased from Applied Biosystems, and primers for amplification were obtained from Sigma-Genosys. .. All reactions were carried out in 5 μl (5 pmol of each primer and 1 pmol of each probe) using the Allelic Discrimination function on a 7500 Fast real-time PCR system (Applied Biosystems) using QuantiTect probe PCR kit (Qiagen).

    Article Title: No influence of the P-glycoprotein polymorphisms MDR1 G2677T/A and C3435T on the virological and immunological response in treatment na?ve HIV-positive patients
    Article Snippet: .. Briefly, DNA was amplified on the LightCycler with the Quantitect Probe PCR Kit (Qiagen, Hilden, Germany) by using the primers 5'-gcaggagttgttgaaatgaaaatg-3' (forward) and 5'-cgcctgctttagtttgactca-3' (reverse). .. Hybridization probes were added to the master mix to a final concentration of 0.05 μM (sensor probe: 5'-ttcccagTaccttct-fluorescein; locked nucleic acid base in upper case letter) and 0.15 μM (anchor probe: 5'-LC Red640-ctttcttatctttcagtgcttgtcc-p).

    Article Title: Detection and Genetic Analysis of Noroviruses and Sapoviruses in Sea Snail
    Article Snippet: .. Real-Time PCR to Quantify NoV and SaV Real-time PCR amplification of the NoV and SaV genomes was performed with a StepOne™ Real-Time PCR System (Applied Biosystems, Foster City, CA, USA), using a QuantiTect Probe PCR Kit (Qiagen). ..

    Positive Control:

    Article Title: Detection and Genetic Analysis of Noroviruses and Sapoviruses in Sea Snail
    Article Snippet: Real-Time PCR to Quantify NoV and SaV Real-time PCR amplification of the NoV and SaV genomes was performed with a StepOne™ Real-Time PCR System (Applied Biosystems, Foster City, CA, USA), using a QuantiTect Probe PCR Kit (Qiagen). .. The samples were analyzed with real-time PCR using standard plasmid (pcDNA3.1/V5-His TOPO; Invitrogen) as the positive control to create the standard curve, and nuclease-free water as the negative control.

    Synthesized:

    Article Title: Recombinant human erythropoietin increases survival and reduces neuronal apoptosis in a murine model of cerebral malaria
    Article Snippet: Quantification of RNA content was done by using the Rediplate Ribogreen kit (Invitrogen, USA) and cDNA was synthesized using the QuantiTect Reverse Transcription Kit (Qiagen, USA). .. All real time-PCR reactions were carried out on a MX-3000p (Stratagene, USA) and run for 50 cycles using the QuantiTect Probe PCR Kit (Qiagen, USA) according to the manufacturers protocol and the following protein specific kits: β-actin, cat. no. 241014, caspase 3, cat. no. 241124, no. 241054, caspase 1, cat. no. 241151, TNF, cat. no. 241034, IFN-γ, cat. no. 241036, IL-1β, cat. and LT: cat no. 241174.

    Article Title: Diacylglycerol Acyltransferase-1 Localizes Hepatitis C Virus NS5A Protein to Lipid Droplets and Enhances NS5A Interaction with the Viral Capsid Core *
    Article Snippet: Total cellular RNA was isolated with RNA Stat reagent (TelTest) according to the manufacturer's protocol and treated with the TURBO DNA-free DNase (Ambion). cDNAs were synthesized with Superscript III reverse transcriptase (Invitrogen) with random hexamer primers. .. Real-time PCR was performed with a QuantiTect Probe PCR Kit (Qiagen) on a 7900HT Fast Real-time RT-PCR System (Applied Biosystems).

    Article Title: MAPK Signaling has Stage-dependent Osteogenic Effects on Human Adipose-derived Stem Cells in vitro
    Article Snippet: OM-induced and inhibitor-treated ASC samples (n=8) were harvested after 21 days and cDNA was synthesized from 1 μg total RNA using Taqman Gold reagents (Applied Biosystems). .. Real-time PCR reactions were prepared using the Quantitect Probe PCR Kit (Qiagen) and run on an ABI 7700 Fastcycler.

    Quantitative RT-PCR:

    Article Title: Assessment of Heat Shock Protein 70 Induction by Heat in Alfalfa Varieties and Constitutive Overexpression in Transgenic Plants
    Article Snippet: .. The expression level of HSC70 and HSP70 in the heat treated samples was verified by real-time quantitative reverse transcription PCR (RT-qPCR) with oligonucleotides designed on the basis of the AtHSC70 (AL162971) and AtHSP70 (AJ002551) sequences [ ], using the Beacon designer 2.0 software (BIO-RAD) and the iCycler iQ Multicolor Real Time PCR detection system (BIO-RAD) with the Quantitect probe PCR kit (Qiagen). ..

    Article Title: Natural Single-Nucleotide Variations in the HIV-1 Genomic SA1prox Region Can Alter Viral Replication Ability by Regulating Vif Expression Levels
    Article Snippet: .. For qRT-PCR analysis, samples used for semiquantitative RT-PCR analysis were subjected to qRT-PCR analysis by using the QuantiTect Probe PCR kit (Qiagen GmbH), using the primer pairs for qRT-PCR analysis of viral transcripts described above. .. Tat trans -activation assays were performed as described previously ( ).

    Article Title: Kaposi's Sarcoma-Associated Herpesvirus Inhibits Expression and Function of Endothelial Cell Major Histocompatibility Complex Class II via Suppressor of Cytokine Signaling 3
    Article Snippet: .. RNA was extracted from EC using TRIzol (Invitrogen), and quantitative reverse transcriptase PCR (qRT-PCR) was conducted either in one step with a QuantiTect probe PCR kit (Qiagen) ( SOCS3 and CIITA ) or in two steps with conversion to cDNA, followed by qPCR, using TaqMan reagents (Applied Biosystems). .. Assay-on-Demand primers and probes were used for SOCS3 , CIITA , and HLA-DRA (Applied Biosystems) and 18S as a control gene (Eurogentec or Applied Biosystems).

    Article Title: Membrane-Free Stem Cell Components Inhibit Interleukin-1α-Stimulated Inflammation and Cartilage Degradation In Vitro and In Vivo: A Rat Model of Osteoarthritis
    Article Snippet: Paragraph title: 4.8. qRT-PCR ... Obtained total RNA was quantified using a NanoDrop spectrophotometer and were reverse-transcribed at 42 °C into cDNA for 30 min using QuantiTect Probe PCR kit (Qiagen, GmbH, Hilden, Germany).

    Article Title: HIV-1 Vpr- and Reverse Transcription-Induced Apoptosis in Resting Peripheral Blood CD4 T Cells and Protection by Common Gamma-Chain Cytokines
    Article Snippet: .. DNA was analyzed using the QuantiTect probe PCR kit (Qiagen), and RNA was analyzed by RT-qPCR using the QuantiTect probe RT-PCR kit (Qiagen). .. Integrated HIV-1 was detect by Alu PCR as described previously ( ).

    Article Title: Diacylglycerol Acyltransferase-1 Localizes Hepatitis C Virus NS5A Protein to Lipid Droplets and Enhances NS5A Interaction with the Viral Capsid Core *
    Article Snippet: .. Real-time PCR was performed with a QuantiTect Probe PCR Kit (Qiagen) on a 7900HT Fast Real-time RT-PCR System (Applied Biosystems). .. Hepatoma cells in 6-well plates were washed with PBS and incubated with 1 ml of hexane:isopropyl alcohol (3:2) on a vertical shaker at 100 rpm, 3 × 10 min.

    Electrophoresis:

    Article Title: Natural Single-Nucleotide Variations in the HIV-1 Genomic SA1prox Region Can Alter Viral Replication Ability by Regulating Vif Expression Levels
    Article Snippet: PCR amplicons were separated on a 2% Metaphor agarose (Lonza Ltd.) gel in 0.5× Tris-borate-EDTA buffer by using the Mupid exU submarine electrophoresis system (Advance Co. Ltd., Tokyo, Japan) and stained with ethidium bromide. .. For qRT-PCR analysis, samples used for semiquantitative RT-PCR analysis were subjected to qRT-PCR analysis by using the QuantiTect Probe PCR kit (Qiagen GmbH), using the primer pairs for qRT-PCR analysis of viral transcripts described above.

    Random Hexamer Labeling:

    Article Title: Diacylglycerol Acyltransferase-1 Localizes Hepatitis C Virus NS5A Protein to Lipid Droplets and Enhances NS5A Interaction with the Viral Capsid Core *
    Article Snippet: Total cellular RNA was isolated with RNA Stat reagent (TelTest) according to the manufacturer's protocol and treated with the TURBO DNA-free DNase (Ambion). cDNAs were synthesized with Superscript III reverse transcriptase (Invitrogen) with random hexamer primers. .. Real-time PCR was performed with a QuantiTect Probe PCR Kit (Qiagen) on a 7900HT Fast Real-time RT-PCR System (Applied Biosystems).

    Luciferase:

    Article Title: Natural Single-Nucleotide Variations in the HIV-1 Genomic SA1prox Region Can Alter Viral Replication Ability by Regulating Vif Expression Levels
    Article Snippet: For qRT-PCR analysis, samples used for semiquantitative RT-PCR analysis were subjected to qRT-PCR analysis by using the QuantiTect Probe PCR kit (Qiagen GmbH), using the primer pairs for qRT-PCR analysis of viral transcripts described above. .. Briefly, Proviral clones and an LTR-driven luciferase reporter clone, 5RLTR-Luc , were cotransfected into HEK 293T cells with Lipofectamine 2000 (Life Technologies Corporation).

    Expressing:

    Article Title: Recombinant human erythropoietin increases survival and reduces neuronal apoptosis in a murine model of cerebral malaria
    Article Snippet: Paragraph title: Gene expression ... All real time-PCR reactions were carried out on a MX-3000p (Stratagene, USA) and run for 50 cycles using the QuantiTect Probe PCR Kit (Qiagen, USA) according to the manufacturers protocol and the following protein specific kits: β-actin, cat. no. 241014, caspase 3, cat. no. 241124, no. 241054, caspase 1, cat. no. 241151, TNF, cat. no. 241034, IFN-γ, cat. no. 241036, IL-1β, cat. and LT: cat no. 241174.

    Article Title: Assessment of Heat Shock Protein 70 Induction by Heat in Alfalfa Varieties and Constitutive Overexpression in Transgenic Plants
    Article Snippet: .. The expression level of HSC70 and HSP70 in the heat treated samples was verified by real-time quantitative reverse transcription PCR (RT-qPCR) with oligonucleotides designed on the basis of the AtHSC70 (AL162971) and AtHSP70 (AJ002551) sequences [ ], using the Beacon designer 2.0 software (BIO-RAD) and the iCycler iQ Multicolor Real Time PCR detection system (BIO-RAD) with the Quantitect probe PCR kit (Qiagen). ..

    Article Title: Natural Single-Nucleotide Variations in the HIV-1 Genomic SA1prox Region Can Alter Viral Replication Ability by Regulating Vif Expression Levels
    Article Snippet: Paragraph title: Analysis of HIV-1 gene expression. ... For qRT-PCR analysis, samples used for semiquantitative RT-PCR analysis were subjected to qRT-PCR analysis by using the QuantiTect Probe PCR kit (Qiagen GmbH), using the primer pairs for qRT-PCR analysis of viral transcripts described above.

    Article Title: Kaposi's Sarcoma-Associated Herpesvirus Inhibits Expression and Function of Endothelial Cell Major Histocompatibility Complex Class II via Suppressor of Cytokine Signaling 3
    Article Snippet: Paragraph title: Evaluation of gene expression by qRT-PCR. ... RNA was extracted from EC using TRIzol (Invitrogen), and quantitative reverse transcriptase PCR (qRT-PCR) was conducted either in one step with a QuantiTect probe PCR kit (Qiagen) ( SOCS3 and CIITA ) or in two steps with conversion to cDNA, followed by qPCR, using TaqMan reagents (Applied Biosystems).

    Derivative Assay:

    Article Title: Differential Plasma MicroRNA Profiles in HBeAg Positive and HBeAg Negative Children with Chronic Hepatitis B
    Article Snippet: RT-PCR was performed using 15 µl of extraction per analysis, the QuantiTect Probe PCR kit (Qiagen, Hilden, Germany), 600 nM forward primer HBCPF (5′-ATCTTATCAACACTTCCGGA-3′, nucleotides 2315–2334), 800 nM reverse primer HBCPR (5′-AGATTGAGATCTTCTGCGAC-3′, nucleotides 2434–2415) and 150 nM FAM labelled Tagman Probe HBCPP (5′-FAM-AGGTCCCCTAGAAGAAGAACTCCCT-TAMRA-3′, nucleotides 2360–2384) targeting a 120 bp region (nucleotides 2315–2434) of the core protein gene in hepatitis B virus. .. The quantification was based on an absolute standard curve derived from a HepG2-2.2.15 cell-suspension, which was calibrated using the WHO standard NIBSC (97/750).

    Hybridization:

    Article Title: No influence of the P-glycoprotein polymorphisms MDR1 G2677T/A and C3435T on the virological and immunological response in treatment na?ve HIV-positive patients
    Article Snippet: Genotype analysis DNA was extracted from 200 μl blood and the MDR1 3435 genotype was determined with genotype specific hybridisation probes and melting curve analysis on the LightCycler (Roche, Mannheim, Germany) as previously described [ ]. .. Briefly, DNA was amplified on the LightCycler with the Quantitect Probe PCR Kit (Qiagen, Hilden, Germany) by using the primers 5'-gcaggagttgttgaaatgaaaatg-3' (forward) and 5'-cgcctgctttagtttgactca-3' (reverse).

    Concentration Assay:

    Article Title: Assessment of Heat Shock Protein 70 Induction by Heat in Alfalfa Varieties and Constitutive Overexpression in Transgenic Plants
    Article Snippet: Total RNA concentration was measured using the NanoDrop 1000 spectrophotometer (Thermo Scientific, USA) and the integrity was checked by agarose gel analysis. .. The expression level of HSC70 and HSP70 in the heat treated samples was verified by real-time quantitative reverse transcription PCR (RT-qPCR) with oligonucleotides designed on the basis of the AtHSC70 (AL162971) and AtHSP70 (AJ002551) sequences [ ], using the Beacon designer 2.0 software (BIO-RAD) and the iCycler iQ Multicolor Real Time PCR detection system (BIO-RAD) with the Quantitect probe PCR kit (Qiagen).

    Article Title: No influence of the P-glycoprotein polymorphisms MDR1 G2677T/A and C3435T on the virological and immunological response in treatment na?ve HIV-positive patients
    Article Snippet: Briefly, DNA was amplified on the LightCycler with the Quantitect Probe PCR Kit (Qiagen, Hilden, Germany) by using the primers 5'-gcaggagttgttgaaatgaaaatg-3' (forward) and 5'-cgcctgctttagtttgactca-3' (reverse). .. Hybridization probes were added to the master mix to a final concentration of 0.05 μM (sensor probe: 5'-ttcccagTaccttct-fluorescein; locked nucleic acid base in upper case letter) and 0.15 μM (anchor probe: 5'-LC Red640-ctttcttatctttcagtgcttgtcc-p).

    Infection:

    Article Title: Recombinant human erythropoietin increases survival and reduces neuronal apoptosis in a murine model of cerebral malaria
    Article Snippet: Gene expression For quantitative PCR, separate groups of mice (n = 27) were either infected as described above and treated with rhEpo (100 U i.p. on day 1,4 and 7 post-inoculation) or vehicle or left uninfected. .. All real time-PCR reactions were carried out on a MX-3000p (Stratagene, USA) and run for 50 cycles using the QuantiTect Probe PCR Kit (Qiagen, USA) according to the manufacturers protocol and the following protein specific kits: β-actin, cat. no. 241014, caspase 3, cat. no. 241124, no. 241054, caspase 1, cat. no. 241151, TNF, cat. no. 241034, IFN-γ, cat. no. 241036, IL-1β, cat. and LT: cat no. 241174.

    Polymerase Chain Reaction:

    Article Title: Recombinant human erythropoietin increases survival and reduces neuronal apoptosis in a murine model of cerebral malaria
    Article Snippet: .. All real time-PCR reactions were carried out on a MX-3000p (Stratagene, USA) and run for 50 cycles using the QuantiTect Probe PCR Kit (Qiagen, USA) according to the manufacturers protocol and the following protein specific kits: β-actin, cat. no. 241014, caspase 3, cat. no. 241124, no. 241054, caspase 1, cat. no. 241151, TNF, cat. no. 241034, IFN-γ, cat. no. 241036, IL-1β, cat. and LT: cat no. 241174. .. Cycle-threshold (Ct) values were defined as the PCR cycle at which amplified product was first detected.

    Article Title: Differential Plasma MicroRNA Profiles in HBeAg Positive and HBeAg Negative Children with Chronic Hepatitis B
    Article Snippet: .. RT-PCR was performed using 15 µl of extraction per analysis, the QuantiTect Probe PCR kit (Qiagen, Hilden, Germany), 600 nM forward primer HBCPF (5′-ATCTTATCAACACTTCCGGA-3′, nucleotides 2315–2334), 800 nM reverse primer HBCPR (5′-AGATTGAGATCTTCTGCGAC-3′, nucleotides 2434–2415) and 150 nM FAM labelled Tagman Probe HBCPP (5′-FAM-AGGTCCCCTAGAAGAAGAACTCCCT-TAMRA-3′, nucleotides 2360–2384) targeting a 120 bp region (nucleotides 2315–2434) of the core protein gene in hepatitis B virus. .. The analyses were run on a Stratagene Mx3005p (Agilent Technologies, Santa Clara, California, USA).

    Article Title: Assessment of Heat Shock Protein 70 Induction by Heat in Alfalfa Varieties and Constitutive Overexpression in Transgenic Plants
    Article Snippet: .. The expression level of HSC70 and HSP70 in the heat treated samples was verified by real-time quantitative reverse transcription PCR (RT-qPCR) with oligonucleotides designed on the basis of the AtHSC70 (AL162971) and AtHSP70 (AJ002551) sequences [ ], using the Beacon designer 2.0 software (BIO-RAD) and the iCycler iQ Multicolor Real Time PCR detection system (BIO-RAD) with the Quantitect probe PCR kit (Qiagen). ..

    Article Title: Prevalence and Phylogenetic Analysis of Mycoplasma Synoviae Strains Isolated from Polish Chicken Layer Flocks
    Article Snippet: .. The reaction was carried out using a QuantiTect Probe PCR Kit (Qiagen, Germany) in a total volume of 25 μl with 1.3 μL of each 10 μM primer (MSF: 5’-CTA AAT ACA ATA GCC CAA GGC AA-3’; MSR: 5’-CCT CCT TTC TTA CGG AGT ACA-3’), 0.5 μL of probe, 7.4 μL of distilled water, and 2 μL of DNA in an ABI 7500 thermal cycler (Applied Biosystems, part of Thermo Fisher Scientific, USA) under the following conditions: 50°C for 2 min, 95°C for 15 min, 40 cycles of 94°C for 30 sec, and 60°C for 1 min. LAMP method . ..

    Article Title: Natural Single-Nucleotide Variations in the HIV-1 Genomic SA1prox Region Can Alter Viral Replication Ability by Regulating Vif Expression Levels
    Article Snippet: .. For qRT-PCR analysis, samples used for semiquantitative RT-PCR analysis were subjected to qRT-PCR analysis by using the QuantiTect Probe PCR kit (Qiagen GmbH), using the primer pairs for qRT-PCR analysis of viral transcripts described above. .. Tat trans -activation assays were performed as described previously ( ).

    Article Title: HECTD2 Is Associated with Susceptibility to Mouse and Human Prion Disease
    Article Snippet: .. Hectd2 real-time PCR was carried out on a 7500 Fast Real-time PCR System (Applied Biosystems) in a total volume of 15 µl using 1 µl cDNA (200–300 ng) and QuantiTect probe PCR kit (Qiagen) according to the manufacturer's instructions. .. Primers (6 pmoles) F- 5′-CCCCTGAGCTAGGCATTTCC-3′ , R- 5′ –GAGTTACTGCACCCTTGAATTCTG-3′ and probe (3 pmoles) 5′-FamTGTCGCTGTGCTTTCTGCACCCAACTamra-3′ were designed using PrimerExpress software (Applied Biosystems) and supplied by Sigma Genosys.

    Article Title: Kaposi's Sarcoma-Associated Herpesvirus Inhibits Expression and Function of Endothelial Cell Major Histocompatibility Complex Class II via Suppressor of Cytokine Signaling 3
    Article Snippet: .. RNA was extracted from EC using TRIzol (Invitrogen), and quantitative reverse transcriptase PCR (qRT-PCR) was conducted either in one step with a QuantiTect probe PCR kit (Qiagen) ( SOCS3 and CIITA ) or in two steps with conversion to cDNA, followed by qPCR, using TaqMan reagents (Applied Biosystems). .. Assay-on-Demand primers and probes were used for SOCS3 , CIITA , and HLA-DRA (Applied Biosystems) and 18S as a control gene (Eurogentec or Applied Biosystems).

    Article Title: Membrane-Free Stem Cell Components Inhibit Interleukin-1α-Stimulated Inflammation and Cartilage Degradation In Vitro and In Vivo: A Rat Model of Osteoarthritis
    Article Snippet: .. Obtained total RNA was quantified using a NanoDrop spectrophotometer and were reverse-transcribed at 42 °C into cDNA for 30 min using QuantiTect Probe PCR kit (Qiagen, GmbH, Hilden, Germany). .. The cDNA was successively amplified by PCR using the PCR Master Mix (Promega, Madison, WI, USA) according to the manufacturer’s instructions in a Thermal Cycler Dice® Real-Time PCR system (TaKaRa Bio, Kyoto, Japan).

    Article Title: Analysis of MicroRNA Expression in Newborns with Differential Birth Weight Using Newborn Screening Cards
    Article Snippet: .. Real Time Quantitative PCR Sequences of specific forward primers are listed on , a universal reverse primer (URP) and universal probe library probe #21 (UPL-21) were the same for all miRNAs, according to Czimmerer et al. method [ ]. qPCR reactions were carried out on a Roche’s LightCycler 2.0 (Roche Diagnostics, Mannheim, Germany) using QuantiTect Probe PCR Kit (Qiagen, Hilden, Germany) in a final volume of 20 µL containing: 5 µL of cDNA dilution, 0.1 µL of 100 µM specific reverse primer, 0.1 µL of 100 µM URP, 0.2 µL of probe UPL #21 (Roche) and 4.6 µL of nuclease free water; PCR reactions were performed by duplicate. .. PCR conditions were: initial denaturation at 95 °C for 10 min, followed by 50 cycles of 95 °C for 10 s, 60 °C for 30 s and 72 °C for 1 s, with a final hold at 40 °C for 10 min.

    Article Title: HIV-1 Vpr- and Reverse Transcription-Induced Apoptosis in Resting Peripheral Blood CD4 T Cells and Protection by Common Gamma-Chain Cytokines
    Article Snippet: .. DNA was analyzed using the QuantiTect probe PCR kit (Qiagen), and RNA was analyzed by RT-qPCR using the QuantiTect probe RT-PCR kit (Qiagen). .. Integrated HIV-1 was detect by Alu PCR as described previously ( ).

    Article Title: A Copine family member, Cpne8, is a candidate quantitative trait gene for prion disease incubation time in mouse
    Article Snippet: .. All reactions were carried out in 5 μl (5 pmol of each primer and 1 pmol of each probe) using the Allelic Discrimination function on a 7500 Fast real-time PCR system (Applied Biosystems) using QuantiTect probe PCR kit (Qiagen). .. Sequencing Genomic DNA for the parental strains were obtained from the Jackson Laboratory (Bar Harbor, Maine, USA).

    Article Title: No influence of the P-glycoprotein polymorphisms MDR1 G2677T/A and C3435T on the virological and immunological response in treatment na?ve HIV-positive patients
    Article Snippet: .. Briefly, DNA was amplified on the LightCycler with the Quantitect Probe PCR Kit (Qiagen, Hilden, Germany) by using the primers 5'-gcaggagttgttgaaatgaaaatg-3' (forward) and 5'-cgcctgctttagtttgactca-3' (reverse). .. Hybridization probes were added to the master mix to a final concentration of 0.05 μM (sensor probe: 5'-ttcccagTaccttct-fluorescein; locked nucleic acid base in upper case letter) and 0.15 μM (anchor probe: 5'-LC Red640-ctttcttatctttcagtgcttgtcc-p).

    Article Title: Diacylglycerol Acyltransferase-1 Localizes Hepatitis C Virus NS5A Protein to Lipid Droplets and Enhances NS5A Interaction with the Viral Capsid Core *
    Article Snippet: .. Real-time PCR was performed with a QuantiTect Probe PCR Kit (Qiagen) on a 7900HT Fast Real-time RT-PCR System (Applied Biosystems). .. Hepatoma cells in 6-well plates were washed with PBS and incubated with 1 ml of hexane:isopropyl alcohol (3:2) on a vertical shaker at 100 rpm, 3 × 10 min.

    Article Title: Detection and Genetic Analysis of Noroviruses and Sapoviruses in Sea Snail
    Article Snippet: .. Real-Time PCR to Quantify NoV and SaV Real-time PCR amplification of the NoV and SaV genomes was performed with a StepOne™ Real-Time PCR System (Applied Biosystems, Foster City, CA, USA), using a QuantiTect Probe PCR Kit (Qiagen). ..

    Article Title: MAPK Signaling has Stage-dependent Osteogenic Effects on Human Adipose-derived Stem Cells in vitro
    Article Snippet: .. Real-time PCR reactions were prepared using the Quantitect Probe PCR Kit (Qiagen) and run on an ABI 7700 Fastcycler. .. Customized primer/probe mixes to Runx-2, Osteonectin (ON), Osteopontin (OP), Osteocalcin (OC) and Alkaline Phosphatase (AP) were purchased from Applied Biosystems (FAM-labelled probes, TAMRA quencher).

    Injection:

    Article Title: A Copine family member, Cpne8, is a candidate quantitative trait gene for prion disease incubation time in mouse
    Article Snippet: Samples were injected at 3 kV for 45 s and run at 10 kV for 60 min. Fragment sizes were analysed using Genetic Profiler v1.1 (GE Healthcare). .. All reactions were carried out in 5 μl (5 pmol of each primer and 1 pmol of each probe) using the Allelic Discrimination function on a 7500 Fast real-time PCR system (Applied Biosystems) using QuantiTect probe PCR kit (Qiagen).

    Cellular Antioxidant Activity Assay:

    Article Title: Prevalence and Phylogenetic Analysis of Mycoplasma Synoviae Strains Isolated from Polish Chicken Layer Flocks
    Article Snippet: .. The reaction was carried out using a QuantiTect Probe PCR Kit (Qiagen, Germany) in a total volume of 25 μl with 1.3 μL of each 10 μM primer (MSF: 5’-CTA AAT ACA ATA GCC CAA GGC AA-3’; MSR: 5’-CCT CCT TTC TTA CGG AGT ACA-3’), 0.5 μL of probe, 7.4 μL of distilled water, and 2 μL of DNA in an ABI 7500 thermal cycler (Applied Biosystems, part of Thermo Fisher Scientific, USA) under the following conditions: 50°C for 2 min, 95°C for 15 min, 40 cycles of 94°C for 30 sec, and 60°C for 1 min. LAMP method . ..

    Isolation:

    Article Title: Recombinant human erythropoietin increases survival and reduces neuronal apoptosis in a murine model of cerebral malaria
    Article Snippet: RNA was isolated from homogenized tissue using a standard RNeasy lipid tissue kit (Qiagen, USA) according to the manufacturers' protocol followed by dilution in first Qiazol lysis reagent (Qiagen, USA) and secondly chloroform 99.9% (Sigma, Denmark) to separate phases. .. All real time-PCR reactions were carried out on a MX-3000p (Stratagene, USA) and run for 50 cycles using the QuantiTect Probe PCR Kit (Qiagen, USA) according to the manufacturers protocol and the following protein specific kits: β-actin, cat. no. 241014, caspase 3, cat. no. 241124, no. 241054, caspase 1, cat. no. 241151, TNF, cat. no. 241034, IFN-γ, cat. no. 241036, IL-1β, cat. and LT: cat no. 241174.

    Article Title: Assessment of Heat Shock Protein 70 Induction by Heat in Alfalfa Varieties and Constitutive Overexpression in Transgenic Plants
    Article Snippet: Paragraph title: Arabidopsis HSP70 RNA isolation and expression analysis ... The expression level of HSC70 and HSP70 in the heat treated samples was verified by real-time quantitative reverse transcription PCR (RT-qPCR) with oligonucleotides designed on the basis of the AtHSC70 (AL162971) and AtHSP70 (AJ002551) sequences [ ], using the Beacon designer 2.0 software (BIO-RAD) and the iCycler iQ Multicolor Real Time PCR detection system (BIO-RAD) with the Quantitect probe PCR kit (Qiagen).

    Article Title: Diacylglycerol Acyltransferase-1 Localizes Hepatitis C Virus NS5A Protein to Lipid Droplets and Enhances NS5A Interaction with the Viral Capsid Core *
    Article Snippet: Paragraph title: RNA Isolation and Real-time RT-PCR ... Real-time PCR was performed with a QuantiTect Probe PCR Kit (Qiagen) on a 7900HT Fast Real-time RT-PCR System (Applied Biosystems).

    Size-exclusion Chromatography:

    Article Title: Prevalence and Phylogenetic Analysis of Mycoplasma Synoviae Strains Isolated from Polish Chicken Layer Flocks
    Article Snippet: .. The reaction was carried out using a QuantiTect Probe PCR Kit (Qiagen, Germany) in a total volume of 25 μl with 1.3 μL of each 10 μM primer (MSF: 5’-CTA AAT ACA ATA GCC CAA GGC AA-3’; MSR: 5’-CCT CCT TTC TTA CGG AGT ACA-3’), 0.5 μL of probe, 7.4 μL of distilled water, and 2 μL of DNA in an ABI 7500 thermal cycler (Applied Biosystems, part of Thermo Fisher Scientific, USA) under the following conditions: 50°C for 2 min, 95°C for 15 min, 40 cycles of 94°C for 30 sec, and 60°C for 1 min. LAMP method . ..

    Mouse Assay:

    Article Title: Recombinant human erythropoietin increases survival and reduces neuronal apoptosis in a murine model of cerebral malaria
    Article Snippet: Mice were perfused trans-cardially with NaCl (0.9%) with 0.3% heparin (15,000 IU/L) for 30 – 60 seconds. .. All real time-PCR reactions were carried out on a MX-3000p (Stratagene, USA) and run for 50 cycles using the QuantiTect Probe PCR Kit (Qiagen, USA) according to the manufacturers protocol and the following protein specific kits: β-actin, cat. no. 241014, caspase 3, cat. no. 241124, no. 241054, caspase 1, cat. no. 241151, TNF, cat. no. 241034, IFN-γ, cat. no. 241036, IL-1β, cat. and LT: cat no. 241174.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Differential Plasma MicroRNA Profiles in HBeAg Positive and HBeAg Negative Children with Chronic Hepatitis B
    Article Snippet: .. RT-PCR was performed using 15 µl of extraction per analysis, the QuantiTect Probe PCR kit (Qiagen, Hilden, Germany), 600 nM forward primer HBCPF (5′-ATCTTATCAACACTTCCGGA-3′, nucleotides 2315–2334), 800 nM reverse primer HBCPR (5′-AGATTGAGATCTTCTGCGAC-3′, nucleotides 2434–2415) and 150 nM FAM labelled Tagman Probe HBCPP (5′-FAM-AGGTCCCCTAGAAGAAGAACTCCCT-TAMRA-3′, nucleotides 2360–2384) targeting a 120 bp region (nucleotides 2315–2434) of the core protein gene in hepatitis B virus. .. The analyses were run on a Stratagene Mx3005p (Agilent Technologies, Santa Clara, California, USA).

    Article Title: Natural Single-Nucleotide Variations in the HIV-1 Genomic SA1prox Region Can Alter Viral Replication Ability by Regulating Vif Expression Levels
    Article Snippet: .. For qRT-PCR analysis, samples used for semiquantitative RT-PCR analysis were subjected to qRT-PCR analysis by using the QuantiTect Probe PCR kit (Qiagen GmbH), using the primer pairs for qRT-PCR analysis of viral transcripts described above. .. Tat trans -activation assays were performed as described previously ( ).

    Article Title: HIV-1 Vpr- and Reverse Transcription-Induced Apoptosis in Resting Peripheral Blood CD4 T Cells and Protection by Common Gamma-Chain Cytokines
    Article Snippet: .. DNA was analyzed using the QuantiTect probe PCR kit (Qiagen), and RNA was analyzed by RT-qPCR using the QuantiTect probe RT-PCR kit (Qiagen). .. Integrated HIV-1 was detect by Alu PCR as described previously ( ).

    Staining:

    Article Title: Natural Single-Nucleotide Variations in the HIV-1 Genomic SA1prox Region Can Alter Viral Replication Ability by Regulating Vif Expression Levels
    Article Snippet: PCR amplicons were separated on a 2% Metaphor agarose (Lonza Ltd.) gel in 0.5× Tris-borate-EDTA buffer by using the Mupid exU submarine electrophoresis system (Advance Co. Ltd., Tokyo, Japan) and stained with ethidium bromide. .. For qRT-PCR analysis, samples used for semiquantitative RT-PCR analysis were subjected to qRT-PCR analysis by using the QuantiTect Probe PCR kit (Qiagen GmbH), using the primer pairs for qRT-PCR analysis of viral transcripts described above.

    Purification:

    Article Title: Prevalence and Phylogenetic Analysis of Mycoplasma Synoviae Strains Isolated from Polish Chicken Layer Flocks
    Article Snippet: Purified DNA samples (100 μL) were stored at −20°C for further downstream molecular analysis. .. The reaction was carried out using a QuantiTect Probe PCR Kit (Qiagen, Germany) in a total volume of 25 μl with 1.3 μL of each 10 μM primer (MSF: 5’-CTA AAT ACA ATA GCC CAA GGC AA-3’; MSR: 5’-CCT CCT TTC TTA CGG AGT ACA-3’), 0.5 μL of probe, 7.4 μL of distilled water, and 2 μL of DNA in an ABI 7500 thermal cycler (Applied Biosystems, part of Thermo Fisher Scientific, USA) under the following conditions: 50°C for 2 min, 95°C for 15 min, 40 cycles of 94°C for 30 sec, and 60°C for 1 min. LAMP method .

    Article Title: HECTD2 Is Associated with Susceptibility to Mouse and Human Prion Disease
    Article Snippet: Samples were treated with DNaseI (Qiagen) and purified further using RNeasy Mini (Qiagen) columns according to the manufacturer's instructions. .. Hectd2 real-time PCR was carried out on a 7500 Fast Real-time PCR System (Applied Biosystems) in a total volume of 15 µl using 1 µl cDNA (200–300 ng) and QuantiTect probe PCR kit (Qiagen) according to the manufacturer's instructions.

    Article Title: HIV-1 Vpr- and Reverse Transcription-Induced Apoptosis in Resting Peripheral Blood CD4 T Cells and Protection by Common Gamma-Chain Cytokines
    Article Snippet: Briefly, DNA from cells was purified using the DNeasy blood and tissue kit or the AllPrep kit (Qiagen). .. DNA was analyzed using the QuantiTect probe PCR kit (Qiagen), and RNA was analyzed by RT-qPCR using the QuantiTect probe RT-PCR kit (Qiagen).

    Plasmid Preparation:

    Article Title: Detection and Genetic Analysis of Noroviruses and Sapoviruses in Sea Snail
    Article Snippet: Real-Time PCR to Quantify NoV and SaV Real-time PCR amplification of the NoV and SaV genomes was performed with a StepOne™ Real-Time PCR System (Applied Biosystems, Foster City, CA, USA), using a QuantiTect Probe PCR Kit (Qiagen). .. The samples were analyzed with real-time PCR using standard plasmid (pcDNA3.1/V5-His TOPO; Invitrogen) as the positive control to create the standard curve, and nuclease-free water as the negative control.

    Software:

    Article Title: Assessment of Heat Shock Protein 70 Induction by Heat in Alfalfa Varieties and Constitutive Overexpression in Transgenic Plants
    Article Snippet: .. The expression level of HSC70 and HSP70 in the heat treated samples was verified by real-time quantitative reverse transcription PCR (RT-qPCR) with oligonucleotides designed on the basis of the AtHSC70 (AL162971) and AtHSP70 (AJ002551) sequences [ ], using the Beacon designer 2.0 software (BIO-RAD) and the iCycler iQ Multicolor Real Time PCR detection system (BIO-RAD) with the Quantitect probe PCR kit (Qiagen). ..

    Article Title: HECTD2 Is Associated with Susceptibility to Mouse and Human Prion Disease
    Article Snippet: Hectd2 real-time PCR was carried out on a 7500 Fast Real-time PCR System (Applied Biosystems) in a total volume of 15 µl using 1 µl cDNA (200–300 ng) and QuantiTect probe PCR kit (Qiagen) according to the manufacturer's instructions. .. Primers (6 pmoles) F- 5′-CCCCTGAGCTAGGCATTTCC-3′ , R- 5′ –GAGTTACTGCACCCTTGAATTCTG-3′ and probe (3 pmoles) 5′-FamTGTCGCTGTGCTTTCTGCACCCAACTamra-3′ were designed using PrimerExpress software (Applied Biosystems) and supplied by Sigma Genosys.

    Article Title: Kaposi's Sarcoma-Associated Herpesvirus Inhibits Expression and Function of Endothelial Cell Major Histocompatibility Complex Class II via Suppressor of Cytokine Signaling 3
    Article Snippet: RNA was extracted from EC using TRIzol (Invitrogen), and quantitative reverse transcriptase PCR (qRT-PCR) was conducted either in one step with a QuantiTect probe PCR kit (Qiagen) ( SOCS3 and CIITA ) or in two steps with conversion to cDNA, followed by qPCR, using TaqMan reagents (Applied Biosystems). .. Samples were amplified using the 7900 HT real-time PCR machine and analyzed using the software package SDS 2.2 (Applied Biosystems).

    Article Title: A Copine family member, Cpne8, is a candidate quantitative trait gene for prion disease incubation time in mouse
    Article Snippet: Primers and probes for genotyping SNPs (Electronic supplementary material, Table ) were designed using criteria defined by Applied Biosystems and PrimerExpress software (Applied Biosystems). .. All reactions were carried out in 5 μl (5 pmol of each primer and 1 pmol of each probe) using the Allelic Discrimination function on a 7500 Fast real-time PCR system (Applied Biosystems) using QuantiTect probe PCR kit (Qiagen).

    Real-time Polymerase Chain Reaction:

    Article Title: Recombinant human erythropoietin increases survival and reduces neuronal apoptosis in a murine model of cerebral malaria
    Article Snippet: .. All real time-PCR reactions were carried out on a MX-3000p (Stratagene, USA) and run for 50 cycles using the QuantiTect Probe PCR Kit (Qiagen, USA) according to the manufacturers protocol and the following protein specific kits: β-actin, cat. no. 241014, caspase 3, cat. no. 241124, no. 241054, caspase 1, cat. no. 241151, TNF, cat. no. 241034, IFN-γ, cat. no. 241036, IL-1β, cat. and LT: cat no. 241174. .. Cycle-threshold (Ct) values were defined as the PCR cycle at which amplified product was first detected.

    Article Title: Differential Plasma MicroRNA Profiles in HBeAg Positive and HBeAg Negative Children with Chronic Hepatitis B
    Article Snippet: HBV DNA was quantified using real-time polymerase-chain-reaction (RT-PCR). .. RT-PCR was performed using 15 µl of extraction per analysis, the QuantiTect Probe PCR kit (Qiagen, Hilden, Germany), 600 nM forward primer HBCPF (5′-ATCTTATCAACACTTCCGGA-3′, nucleotides 2315–2334), 800 nM reverse primer HBCPR (5′-AGATTGAGATCTTCTGCGAC-3′, nucleotides 2434–2415) and 150 nM FAM labelled Tagman Probe HBCPP (5′-FAM-AGGTCCCCTAGAAGAAGAACTCCCT-TAMRA-3′, nucleotides 2360–2384) targeting a 120 bp region (nucleotides 2315–2434) of the core protein gene in hepatitis B virus.

    Article Title: Assessment of Heat Shock Protein 70 Induction by Heat in Alfalfa Varieties and Constitutive Overexpression in Transgenic Plants
    Article Snippet: .. The expression level of HSC70 and HSP70 in the heat treated samples was verified by real-time quantitative reverse transcription PCR (RT-qPCR) with oligonucleotides designed on the basis of the AtHSC70 (AL162971) and AtHSP70 (AJ002551) sequences [ ], using the Beacon designer 2.0 software (BIO-RAD) and the iCycler iQ Multicolor Real Time PCR detection system (BIO-RAD) with the Quantitect probe PCR kit (Qiagen). ..

    Article Title: HECTD2 Is Associated with Susceptibility to Mouse and Human Prion Disease
    Article Snippet: .. Hectd2 real-time PCR was carried out on a 7500 Fast Real-time PCR System (Applied Biosystems) in a total volume of 15 µl using 1 µl cDNA (200–300 ng) and QuantiTect probe PCR kit (Qiagen) according to the manufacturer's instructions. .. Primers (6 pmoles) F- 5′-CCCCTGAGCTAGGCATTTCC-3′ , R- 5′ –GAGTTACTGCACCCTTGAATTCTG-3′ and probe (3 pmoles) 5′-FamTGTCGCTGTGCTTTCTGCACCCAACTamra-3′ were designed using PrimerExpress software (Applied Biosystems) and supplied by Sigma Genosys.

    Article Title: Kaposi's Sarcoma-Associated Herpesvirus Inhibits Expression and Function of Endothelial Cell Major Histocompatibility Complex Class II via Suppressor of Cytokine Signaling 3
    Article Snippet: .. RNA was extracted from EC using TRIzol (Invitrogen), and quantitative reverse transcriptase PCR (qRT-PCR) was conducted either in one step with a QuantiTect probe PCR kit (Qiagen) ( SOCS3 and CIITA ) or in two steps with conversion to cDNA, followed by qPCR, using TaqMan reagents (Applied Biosystems). .. Assay-on-Demand primers and probes were used for SOCS3 , CIITA , and HLA-DRA (Applied Biosystems) and 18S as a control gene (Eurogentec or Applied Biosystems).

    Article Title: Membrane-Free Stem Cell Components Inhibit Interleukin-1α-Stimulated Inflammation and Cartilage Degradation In Vitro and In Vivo: A Rat Model of Osteoarthritis
    Article Snippet: Obtained total RNA was quantified using a NanoDrop spectrophotometer and were reverse-transcribed at 42 °C into cDNA for 30 min using QuantiTect Probe PCR kit (Qiagen, GmbH, Hilden, Germany). .. The cDNA was successively amplified by PCR using the PCR Master Mix (Promega, Madison, WI, USA) according to the manufacturer’s instructions in a Thermal Cycler Dice® Real-Time PCR system (TaKaRa Bio, Kyoto, Japan).

    Article Title: Analysis of MicroRNA Expression in Newborns with Differential Birth Weight Using Newborn Screening Cards
    Article Snippet: .. Real Time Quantitative PCR Sequences of specific forward primers are listed on , a universal reverse primer (URP) and universal probe library probe #21 (UPL-21) were the same for all miRNAs, according to Czimmerer et al. method [ ]. qPCR reactions were carried out on a Roche’s LightCycler 2.0 (Roche Diagnostics, Mannheim, Germany) using QuantiTect Probe PCR Kit (Qiagen, Hilden, Germany) in a final volume of 20 µL containing: 5 µL of cDNA dilution, 0.1 µL of 100 µM specific reverse primer, 0.1 µL of 100 µM URP, 0.2 µL of probe UPL #21 (Roche) and 4.6 µL of nuclease free water; PCR reactions were performed by duplicate. .. PCR conditions were: initial denaturation at 95 °C for 10 min, followed by 50 cycles of 95 °C for 10 s, 60 °C for 30 s and 72 °C for 1 s, with a final hold at 40 °C for 10 min.

    Article Title: A Copine family member, Cpne8, is a candidate quantitative trait gene for prion disease incubation time in mouse
    Article Snippet: .. All reactions were carried out in 5 μl (5 pmol of each primer and 1 pmol of each probe) using the Allelic Discrimination function on a 7500 Fast real-time PCR system (Applied Biosystems) using QuantiTect probe PCR kit (Qiagen). .. Sequencing Genomic DNA for the parental strains were obtained from the Jackson Laboratory (Bar Harbor, Maine, USA).

    Article Title: Diacylglycerol Acyltransferase-1 Localizes Hepatitis C Virus NS5A Protein to Lipid Droplets and Enhances NS5A Interaction with the Viral Capsid Core *
    Article Snippet: .. Real-time PCR was performed with a QuantiTect Probe PCR Kit (Qiagen) on a 7900HT Fast Real-time RT-PCR System (Applied Biosystems). .. Hepatoma cells in 6-well plates were washed with PBS and incubated with 1 ml of hexane:isopropyl alcohol (3:2) on a vertical shaker at 100 rpm, 3 × 10 min.

    Article Title: Detection and Genetic Analysis of Noroviruses and Sapoviruses in Sea Snail
    Article Snippet: .. Real-Time PCR to Quantify NoV and SaV Real-time PCR amplification of the NoV and SaV genomes was performed with a StepOne™ Real-Time PCR System (Applied Biosystems, Foster City, CA, USA), using a QuantiTect Probe PCR Kit (Qiagen). ..

    Article Title: MAPK Signaling has Stage-dependent Osteogenic Effects on Human Adipose-derived Stem Cells in vitro
    Article Snippet: .. Real-time PCR reactions were prepared using the Quantitect Probe PCR Kit (Qiagen) and run on an ABI 7700 Fastcycler. .. Customized primer/probe mixes to Runx-2, Osteonectin (ON), Osteopontin (OP), Osteocalcin (OC) and Alkaline Phosphatase (AP) were purchased from Applied Biosystems (FAM-labelled probes, TAMRA quencher).

    Negative Control:

    Article Title: Analysis of MicroRNA Expression in Newborns with Differential Birth Weight Using Newborn Screening Cards
    Article Snippet: Real Time Quantitative PCR Sequences of specific forward primers are listed on , a universal reverse primer (URP) and universal probe library probe #21 (UPL-21) were the same for all miRNAs, according to Czimmerer et al. method [ ]. qPCR reactions were carried out on a Roche’s LightCycler 2.0 (Roche Diagnostics, Mannheim, Germany) using QuantiTect Probe PCR Kit (Qiagen, Hilden, Germany) in a final volume of 20 µL containing: 5 µL of cDNA dilution, 0.1 µL of 100 µM specific reverse primer, 0.1 µL of 100 µM URP, 0.2 µL of probe UPL #21 (Roche) and 4.6 µL of nuclease free water; PCR reactions were performed by duplicate. .. There was no detection signal in non-template control (NTC) or omitting reverse transcriptase enzyme reaction (negative control) after 50 cycles of amplification.

    Article Title: Detection and Genetic Analysis of Noroviruses and Sapoviruses in Sea Snail
    Article Snippet: Real-Time PCR to Quantify NoV and SaV Real-time PCR amplification of the NoV and SaV genomes was performed with a StepOne™ Real-Time PCR System (Applied Biosystems, Foster City, CA, USA), using a QuantiTect Probe PCR Kit (Qiagen). .. The samples were analyzed with real-time PCR using standard plasmid (pcDNA3.1/V5-His TOPO; Invitrogen) as the positive control to create the standard curve, and nuclease-free water as the negative control.

    Agarose Gel Electrophoresis:

    Article Title: Assessment of Heat Shock Protein 70 Induction by Heat in Alfalfa Varieties and Constitutive Overexpression in Transgenic Plants
    Article Snippet: Total RNA concentration was measured using the NanoDrop 1000 spectrophotometer (Thermo Scientific, USA) and the integrity was checked by agarose gel analysis. .. The expression level of HSC70 and HSP70 in the heat treated samples was verified by real-time quantitative reverse transcription PCR (RT-qPCR) with oligonucleotides designed on the basis of the AtHSC70 (AL162971) and AtHSP70 (AJ002551) sequences [ ], using the Beacon designer 2.0 software (BIO-RAD) and the iCycler iQ Multicolor Real Time PCR detection system (BIO-RAD) with the Quantitect probe PCR kit (Qiagen).

    Spectrophotometry:

    Article Title: Assessment of Heat Shock Protein 70 Induction by Heat in Alfalfa Varieties and Constitutive Overexpression in Transgenic Plants
    Article Snippet: Total RNA concentration was measured using the NanoDrop 1000 spectrophotometer (Thermo Scientific, USA) and the integrity was checked by agarose gel analysis. .. The expression level of HSC70 and HSP70 in the heat treated samples was verified by real-time quantitative reverse transcription PCR (RT-qPCR) with oligonucleotides designed on the basis of the AtHSC70 (AL162971) and AtHSP70 (AJ002551) sequences [ ], using the Beacon designer 2.0 software (BIO-RAD) and the iCycler iQ Multicolor Real Time PCR detection system (BIO-RAD) with the Quantitect probe PCR kit (Qiagen).

    Article Title: Membrane-Free Stem Cell Components Inhibit Interleukin-1α-Stimulated Inflammation and Cartilage Degradation In Vitro and In Vivo: A Rat Model of Osteoarthritis
    Article Snippet: .. Obtained total RNA was quantified using a NanoDrop spectrophotometer and were reverse-transcribed at 42 °C into cDNA for 30 min using QuantiTect Probe PCR kit (Qiagen, GmbH, Hilden, Germany). .. The cDNA was successively amplified by PCR using the PCR Master Mix (Promega, Madison, WI, USA) according to the manufacturer’s instructions in a Thermal Cycler Dice® Real-Time PCR system (TaKaRa Bio, Kyoto, Japan).

    Activation Assay:

    Article Title: Natural Single-Nucleotide Variations in the HIV-1 Genomic SA1prox Region Can Alter Viral Replication Ability by Regulating Vif Expression Levels
    Article Snippet: For qRT-PCR analysis, samples used for semiquantitative RT-PCR analysis were subjected to qRT-PCR analysis by using the QuantiTect Probe PCR kit (Qiagen GmbH), using the primer pairs for qRT-PCR analysis of viral transcripts described above. .. Tat trans -activation assays were performed as described previously ( ).

    Lysis:

    Article Title: Recombinant human erythropoietin increases survival and reduces neuronal apoptosis in a murine model of cerebral malaria
    Article Snippet: RNA was isolated from homogenized tissue using a standard RNeasy lipid tissue kit (Qiagen, USA) according to the manufacturers' protocol followed by dilution in first Qiazol lysis reagent (Qiagen, USA) and secondly chloroform 99.9% (Sigma, Denmark) to separate phases. .. All real time-PCR reactions were carried out on a MX-3000p (Stratagene, USA) and run for 50 cycles using the QuantiTect Probe PCR Kit (Qiagen, USA) according to the manufacturers protocol and the following protein specific kits: β-actin, cat. no. 241014, caspase 3, cat. no. 241124, no. 241054, caspase 1, cat. no. 241151, TNF, cat. no. 241034, IFN-γ, cat. no. 241036, IL-1β, cat. and LT: cat no. 241174.

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    Qiagen quantitect probe pcr kit
    Quantitect Probe Pcr Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 134 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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