hsa mir 342 3p  (Qiagen)

 
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    Name:
    QuantiTect Probe PCR Kit
    Description:
    For real time PCR and two step RT PCR using sequence specific probes Kit contents Qiagen QuantiTect Probe PCR Kit 200 x 50L rxns DNA cDNA RNA Sample PCR and Two step RT PCR Reaction Sequence specific Probes Ideal for Real time Quantification of DNA cDNA or RNA Targets For Real time PCR and Two step RT PCR Using Sequence specific Probes Includes 3 x 1 7mL 2x QuantiTect Probe PCR Master Mix 2 x 2mL RNase free Water Benefits Highly sensitive detection of low copy targets Accurate quantification over several logs of template Use of any sequence specific probe on any real time cycler Available with or without uracil N glycosylase UNG No need to optimize reaction and cycling cond
    Catalog Number:
    204343
    Price:
    460
    Category:
    QuantiTect Probe PCR Kits
    Buy from Supplier


    Structured Review

    Qiagen hsa mir 342 3p
    QuantiTect Probe PCR Kit
    For real time PCR and two step RT PCR using sequence specific probes Kit contents Qiagen QuantiTect Probe PCR Kit 200 x 50L rxns DNA cDNA RNA Sample PCR and Two step RT PCR Reaction Sequence specific Probes Ideal for Real time Quantification of DNA cDNA or RNA Targets For Real time PCR and Two step RT PCR Using Sequence specific Probes Includes 3 x 1 7mL 2x QuantiTect Probe PCR Master Mix 2 x 2mL RNase free Water Benefits Highly sensitive detection of low copy targets Accurate quantification over several logs of template Use of any sequence specific probe on any real time cycler Available with or without uracil N glycosylase UNG No need to optimize reaction and cycling cond
    https://www.bioz.com/result/hsa mir 342 3p/product/Qiagen
    Average 85 stars, based on 387 article reviews
    Price from $9.99 to $1999.99
    hsa mir 342 3p - by Bioz Stars, 2020-07
    85/100 stars

    Images

    1) Product Images from "Population differences in microRNA expression and biological implications"

    Article Title: Population differences in microRNA expression and biological implications

    Journal: RNA Biology

    doi: 10.4161/rna.8.4.16029

    The relationship between the expression of miR-342-3p and ANKRD49 in CEU and YRI samples. (A) The relationship between the expression of miR-342-3p and ANKRD49 in the discovery CEU I/II (open square) and YRI I/II (solid triangle) samples. miR-342-3p expression
    Figure Legend Snippet: The relationship between the expression of miR-342-3p and ANKRD49 in CEU and YRI samples. (A) The relationship between the expression of miR-342-3p and ANKRD49 in the discovery CEU I/II (open square) and YRI I/II (solid triangle) samples. miR-342-3p expression

    Techniques Used: Expressing

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    Real-time Polymerase Chain Reaction:

    Article Title: Estrogen Induces Global Reorganization of Chromatin Structure in Human Breast Cancer Cells
    Article Snippet: .. Real-time PCR was performed on a StepOnePlus Real-Time PCR System apparatus (Applied Biosystems) using the TaqMan technology (QuantiTect Probe PCR Master Mix, Qiagen). .. We used a 5′FAM-3′BHQ1 oligonucleotidic probe (Invitrogen).

    Article Title: Morpholino Antisense Oligomers as a Potential Therapeutic Option for the Correction of Alternative Splicing in PMD, SPG2, and HEMS
    Article Snippet: .. PrimeTime predesigned probe-based qPCR assays (IDT) were used with QuantiTect Probe PCR Master mix (QIAGEN), following the manufacturer’s instructions, to assess transcript levels on inflammatory markers chemokine (C-C motif) ligand 3 (Ccl3 , Mm.PT.58.29283216), cystatin F (Cst7 , Mm.PT.58.8810317), glial fibrillary acidic protein (Gfap , Mm.PT.58.6609337), and integrin alpha X (Itgax , Mm.PT.58.42516719). ..

    Article Title: The Antiviral Alkaloid Berberine Reduces Chikungunya Virus-Induced Mitogen-Activated Protein Kinase Signaling
    Article Snippet: .. Quantification of CHIKV viral RNA copies with primers specific for nsP1 was performed using QuantiTect Probe reverse transcription-PCR (RT-PCR) (Qiagen) and the 7900HT Fast real-time PCR system machine (Applied Biosystems) under conditions described previously ( , ). .. Mice were euthanized, and joint footpad samples were removed and stored in TRIzol (Invitrogen) at −80°C.

    Article Title: Genome-wide analysis uncovers high frequency, strong differential chromosomal interactions and their associated epigenetic patterns in E2-mediated gene regulation
    Article Snippet: .. Real-time PCR was performed on a 7500 Real-Time PCR System apparatus (Applied Biosystems) using the TaqMan technology (QuantiTect Probe PCR Master Mix, Qiagen). ..

    Polymerase Chain Reaction:

    Article Title: Estrogen Induces Global Reorganization of Chromatin Structure in Human Breast Cancer Cells
    Article Snippet: .. Real-time PCR was performed on a StepOnePlus Real-Time PCR System apparatus (Applied Biosystems) using the TaqMan technology (QuantiTect Probe PCR Master Mix, Qiagen). .. We used a 5′FAM-3′BHQ1 oligonucleotidic probe (Invitrogen).

    Article Title: Molecular Detection of Cyclospora cayetanensis in Human Stool Specimens using UNEX-based DNA extraction and real-time PCR
    Article Snippet: .. The results presented here were obtained using QuantiTect Probe PCR Master Mix (QIAGEN) instead of AmpliTaq Master Mix with BSA. .. We improved the specificity of the Verweij assay by making two modifications: 1) increasing the annealing/extension temperature from 60°C to 67°C, and 2) correcting the reverse primer sequence to 5’-AAT GCC ACG GTA GGC CAA TA-3’ (the reverse primer sequence in the original publication by Verweij et al. was missing a base, in comparison with the target gene).

    Article Title: Morpholino Antisense Oligomers as a Potential Therapeutic Option for the Correction of Alternative Splicing in PMD, SPG2, and HEMS
    Article Snippet: .. PrimeTime predesigned probe-based qPCR assays (IDT) were used with QuantiTect Probe PCR Master mix (QIAGEN), following the manufacturer’s instructions, to assess transcript levels on inflammatory markers chemokine (C-C motif) ligand 3 (Ccl3 , Mm.PT.58.29283216), cystatin F (Cst7 , Mm.PT.58.8810317), glial fibrillary acidic protein (Gfap , Mm.PT.58.6609337), and integrin alpha X (Itgax , Mm.PT.58.42516719). ..

    Article Title: Rescue of germline transmission from chimeras by IVF after sperm analysis
    Article Snippet: .. Briefly, 1 μL of each DNA sample was tested in a total reaction volume of 10 μl in quadruplicate utilizing Qiagen QuantiTect PCR kit with ROX chemistry. .. Plates with 384 wells each were processed on an AB7900HT system with the following cycles: 95 °C for 15 min and then 40 cycles of 95 °C for 30 s, 60 °C for 1 min. Primers and probe for detection of mutant sequence (LacZ) in the final concentration of each reaction were forward primer ATCAGGATATG TGGCGGATGA (0.84 μM), reverse primer TGATT TGTGTAGTCGGTTTATGCA (0.84 μM) and probe 6FAM-CGGCATTTTCCGTG-MGB/NFQ (0.28 μM).

    Article Title: HDAC1 Expression in Invasive Ductal Carcinoma of the Breast and Its Value as a Good Prognostic Factor
    Article Snippet: .. The PCR reaction mixture was a mixture of 400 ng cDNA, 5 µL 2× QuantiTect probe PCR master mix (Qiagen), 10 pmol primer, and 30 pmol probe. .. PCR was performed using the RotorGene real-time Q-PCR system (Corbett, Sydney, Australia).

    Article Title: Hypoxemia is an independent predictor of bronchiolitis obliterans following respiratory adenoviral infection in children
    Article Snippet: .. Diagnosis of adenovirus infection PCRs with DNA targets utilized 10 μl purified nucleic acid from Qiagen Quantitect Probe PCR kit (Qiagen, Crawley, UK). .. Thermal cycling conditions were as described previously, except for removal of the 50 °C hold for reverse transcription, and the enzyme activation hold at 95 °C was extended to fifteen minutes for the Qiagen Quantitect Probe PCR kit.

    Article Title: Genome-wide analysis uncovers high frequency, strong differential chromosomal interactions and their associated epigenetic patterns in E2-mediated gene regulation
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    Reverse Transcription Polymerase Chain Reaction:

    Article Title: The Antiviral Alkaloid Berberine Reduces Chikungunya Virus-Induced Mitogen-Activated Protein Kinase Signaling
    Article Snippet: .. Quantification of CHIKV viral RNA copies with primers specific for nsP1 was performed using QuantiTect Probe reverse transcription-PCR (RT-PCR) (Qiagen) and the 7900HT Fast real-time PCR system machine (Applied Biosystems) under conditions described previously ( , ). .. Mice were euthanized, and joint footpad samples were removed and stored in TRIzol (Invitrogen) at −80°C.

    Infection:

    Article Title: Hypoxemia is an independent predictor of bronchiolitis obliterans following respiratory adenoviral infection in children
    Article Snippet: .. Diagnosis of adenovirus infection PCRs with DNA targets utilized 10 μl purified nucleic acid from Qiagen Quantitect Probe PCR kit (Qiagen, Crawley, UK). .. Thermal cycling conditions were as described previously, except for removal of the 50 °C hold for reverse transcription, and the enzyme activation hold at 95 °C was extended to fifteen minutes for the Qiagen Quantitect Probe PCR kit.

    Purification:

    Article Title: Hypoxemia is an independent predictor of bronchiolitis obliterans following respiratory adenoviral infection in children
    Article Snippet: .. Diagnosis of adenovirus infection PCRs with DNA targets utilized 10 μl purified nucleic acid from Qiagen Quantitect Probe PCR kit (Qiagen, Crawley, UK). .. Thermal cycling conditions were as described previously, except for removal of the 50 °C hold for reverse transcription, and the enzyme activation hold at 95 °C was extended to fifteen minutes for the Qiagen Quantitect Probe PCR kit.

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  • 93
    Qiagen quantitect probe pcr kit
    Quantitect Probe Pcr Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 93/100, based on 309 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quantitect probe pcr kit/product/Qiagen
    Average 93 stars, based on 309 article reviews
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