hotstartaq master mix kit  (Qiagen)


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    Name:
    HotStarTaq Master Mix Kit
    Description:
    For highly specific amplification for any PCR application Kit contents Qiagen HotStarTaq Master Mix Kit 250U 5U L 10 min at 97C 60 min at 94C Half life Genomic DNA and cDNA Sample Recombinant Enzyme PCR Amplification Ready to use Ideal for Highly Specific Amplification for any PCR Application Includes 3 x 0 85mL HotStarTaq Master Mix Contains 250U HotStarTaq DNA Polymerase PCR Buffer with 3mM MgCl2 and 400mM of Each dNTP and 2 x 1 7mL RNase free Water Benefits High PCR specificity without the need for optimization Easy reaction setup at room temperature Ready to use master mix format reduces pipetting step
    Catalog Number:
    203443
    Price:
    220
    Category:
    HotStarTaq Master Mix Kit
    Buy from Supplier


    Structured Review

    Qiagen hotstartaq master mix kit
    HotStarTaq Master Mix Kit
    For highly specific amplification for any PCR application Kit contents Qiagen HotStarTaq Master Mix Kit 250U 5U L 10 min at 97C 60 min at 94C Half life Genomic DNA and cDNA Sample Recombinant Enzyme PCR Amplification Ready to use Ideal for Highly Specific Amplification for any PCR Application Includes 3 x 0 85mL HotStarTaq Master Mix Contains 250U HotStarTaq DNA Polymerase PCR Buffer with 3mM MgCl2 and 400mM of Each dNTP and 2 x 1 7mL RNase free Water Benefits High PCR specificity without the need for optimization Easy reaction setup at room temperature Ready to use master mix format reduces pipetting step
    https://www.bioz.com/result/hotstartaq master mix kit/product/Qiagen
    Average 90 stars, based on 264 article reviews
    Price from $9.99 to $1999.99
    hotstartaq master mix kit - by Bioz Stars, 2020-01
    90/100 stars

    Images

    1) Product Images from "Zygosity Determination in Hairless Mice by PCR Based onHrhr Gene Analysis"

    Article Title: Zygosity Determination in Hairless Mice by PCR Based onHrhr Gene Analysis

    Journal: Experimental Animals

    doi: 10.1538/expanim.62.267

    Analysis of Hr alleles in homozygous (H) and wild-type (W) HR mice. The normal (wild-type) Hr allele is ~19 kbp in length and consists of 19 exons. A total of 15 overlapping PCRs covering the entire Hr coding sequence revealed that an insertion mutation was localized in intron 6 of the Hr x allele. KOD FX neo was used for PCR amplification of regions 2–7, and HotStarTaq was used for PCR of other regions. PCRs shown in gray typeface (1, 2, 4, 6, 9, 12, 14, 15), no difference between homozygous and wild-type HR mice. PCRs shown in black typeface (3, 5, 7, 8, 10, 11, 13), no band or different bands were obtained in homozygous HR mice. Six agarose gel electropherograms show the band patterns of all PCRs. The primer sets used were: (1) F224 and R1151, (2) F193 and R1873, (3) F193 and R2433, (4) F2463 and R3455, (5) F1843 and R2433, (6) F2052 and R2433, (7) F1843 and R2078, (8) F1843 and R1972, (9) F1913 and R2078, (10) F1843 and Int6-R1458, (11) F1843 and Int6-R979, (12) Int6-F1290 and R1972, (13) F1843 and Int6-R850, (14) F1843 and Int6-R642, and (15) F1843 and Int6-R539. The primer sequences and elongation time are shown in Tables 1 and 2 , respectively. The primer positions for long PCR shown in Fig. 2 are also indicated in this figure.
    Figure Legend Snippet: Analysis of Hr alleles in homozygous (H) and wild-type (W) HR mice. The normal (wild-type) Hr allele is ~19 kbp in length and consists of 19 exons. A total of 15 overlapping PCRs covering the entire Hr coding sequence revealed that an insertion mutation was localized in intron 6 of the Hr x allele. KOD FX neo was used for PCR amplification of regions 2–7, and HotStarTaq was used for PCR of other regions. PCRs shown in gray typeface (1, 2, 4, 6, 9, 12, 14, 15), no difference between homozygous and wild-type HR mice. PCRs shown in black typeface (3, 5, 7, 8, 10, 11, 13), no band or different bands were obtained in homozygous HR mice. Six agarose gel electropherograms show the band patterns of all PCRs. The primer sets used were: (1) F224 and R1151, (2) F193 and R1873, (3) F193 and R2433, (4) F2463 and R3455, (5) F1843 and R2433, (6) F2052 and R2433, (7) F1843 and R2078, (8) F1843 and R1972, (9) F1913 and R2078, (10) F1843 and Int6-R1458, (11) F1843 and Int6-R979, (12) Int6-F1290 and R1972, (13) F1843 and Int6-R850, (14) F1843 and Int6-R642, and (15) F1843 and Int6-R539. The primer sequences and elongation time are shown in Tables 1 and 2 , respectively. The primer positions for long PCR shown in Fig. 2 are also indicated in this figure.

    Techniques Used: Mouse Assay, Sequencing, Mutagenesis, Polymerase Chain Reaction, Amplification, Agarose Gel Electrophoresis

    Related Articles

    Clone Assay:

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    Amplification:

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    Article Title: Progastrin-releasing peptide and gastrin-releasing peptide receptor mRNA expression in non-tumor tissues of the human gastrointestinal tract
    Article Snippet: Paragraph title: PCR- amplification of GRP and GRP-receptor ss-cDNA ... PCR was performed using a HotStarTaq Master mix kit (Qiagen, Hilden, Germany) in a final reaction volume of 25 μL.

    Article Title: The 6-Kilodalton Early Secreted Antigenic Target-Responsive, Asymptomatic Contacts of Tuberculosis Patients Express Elevated Levels of Interleukin-4 and Reduced Levels of Gamma Interferon
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    Synthesized:

    Article Title: Neural stem cells express melatonin receptors and neurotrophic factors: colocalization of the MT1 receptor with neuronal and glial markers
    Article Snippet: After DNase treatment, cDNA was synthesized from 1–2 μg of total RNA using the Omniscript reverse transcriptase kit (Qiagen Inc., Mississauga, ON) and oligo dT primers. .. PCR was carried out using 1.5 μl (or 3 μl for melatonin MT1 and MT2 receptors) of the RT product and the HotStarTaq master mix kit (Qiagen Inc., Mississauga, ON), together with appropriate primers (Table ).

    Article Title: Cysteine Peptidases, Secreted by Trichomonas gallinae, Are Involved in the Cytopathogenic Effects on a Permanent Chicken Liver Cell Culture
    Article Snippet: All primers were synthesized by Eurofins MWG Operon Ebersberg, Germany. .. Hot start procedures were used for PCR amplification using the “HotStarTaq Master Mix Kit” (Qiagen, Vienna, Austria).

    Lambda DNA Preparation:

    Article Title: Attachment of oligonucleotide probes to poly carbodiimide-coated glass for microarray applications
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    Quantitative RT-PCR:

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    Electrophoresis:

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    Plasmid Purification:

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    Incubation:

    Article Title: Neural stem cells express melatonin receptors and neurotrophic factors: colocalization of the MT1 receptor with neuronal and glial markers
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    Electron Microscopy:

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    Cell Culture:

    Article Title: High Frequency of a Novel Filamentous Phage, VCY?, within an Environmental Vibrio cholerae Population
    Article Snippet: .. In a typical reaction mixture, 20 ng genomic DNA or 2 μl 1:10-diluted cultured strains were used as the template and mixed with 0.4 μM RF-specific or IF-specific primers and a polymerase mixture from the Qiagen HotStarTaq Master Mix Kit using a three-step cycling program consisting of initial denaturation at 95°C for 15 min; 30 cycles of a three-step procedure including denaturation at 94°C for 30s, annealing at 52°C for 30s, and extension at 72°C for 30s; and a final extension at 72°C for 5 min. .. The PCR products were separated on a 1.8% agarose gel prepared with 0.5× Tris-borate-EDTA buffer.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Neural stem cells express melatonin receptors and neurotrophic factors: colocalization of the MT1 receptor with neuronal and glial markers
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    Article Title: Gene expression profile of the nucleus accumbens of human cocaine abusers: evidence for dysregulation of myelin
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    Generated:

    Article Title: The molecular function and clinical phenotype of partial deletions of the IGF2/H19 imprinting control region depends on the spatial arrangement of the remaining CTCF-binding sites
    Article Snippet: Generation of bisulfite amplicon libraries Locus-specific amplicon libraries for each individual were generated. .. First, PCR on bisulfite treated DNA was performed with tagged primers using the Qiagen HotStarTaq Master Mix Kit (Qiagen, Hilden, Germany) and standard protocols.

    Article Title: Concurrent genotyping of Helicobacter pylori virulence genes and human cytokine SNP sites using whole genome amplified DNA derived from minute amounts of gastric biopsy specimen DNA
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    other:

    Article Title: Pancreatic Physiology/Pathophysiology: P21-activated kinase 4 in pancreatic acinar cells is activated by numerous gastrointestinal hormones/neurotransmitters and growth factors by novel signaling, and its activation stimulates secretory/growth cascades
    Article Snippet: RNeasy Mini Kit, DNase Digestion, and HotStarTaq Master Mix Kit were from Qiagen (Valencia, CA).

    Polymerase Chain Reaction:

    Article Title: Neural stem cells express melatonin receptors and neurotrophic factors: colocalization of the MT1 receptor with neuronal and glial markers
    Article Snippet: .. PCR was carried out using 1.5 μl (or 3 μl for melatonin MT1 and MT2 receptors) of the RT product and the HotStarTaq master mix kit (Qiagen Inc., Mississauga, ON), together with appropriate primers (Table ). .. Following a hot start at 95°C for 15 min, samples were amplified for 36 cycles (or 38 cycles for MT1 and MT2 ) at 94°C for 30 s, 57°C for 30 s and 72°C for 1 min, followed by a final incubation at 72°C for 10 min.

    Article Title: The molecular function and clinical phenotype of partial deletions of the IGF2/H19 imprinting control region depends on the spatial arrangement of the remaining CTCF-binding sites
    Article Snippet: .. First, PCR on bisulfite treated DNA was performed with tagged primers using the Qiagen HotStarTaq Master Mix Kit (Qiagen, Hilden, Germany) and standard protocols. ..

    Article Title: Gene expression profile of the nucleus accumbens of human cocaine abusers: evidence for dysregulation of myelin
    Article Snippet: .. Because of the lower basal transcript abundance and greater inducibility of CART, 15 ng input RNA was used for the RT reaction, PCR was performed using the Qiagen HotStarTaq Master Mix Kit, and transcript abundance was quantified using an Agilent DNA 500 LabChip Kit on the Agilent Bioanalyzer 2100. .. For sample normalization, individual transcript values were divided by the subject’s β-actin values determined using the same method. β-Actin transcript levels did not differ between cocaine abusers and control subjects, as determined by either RT–PCR ( p = 0.6468) or microarray ( p = 0.6641).

    Article Title: High Frequency of a Novel Filamentous Phage, VCY?, within an Environmental Vibrio cholerae Population
    Article Snippet: Paragraph title: PCR-based phage identification and screening for the RF or IF in host cells. ... In a typical reaction mixture, 20 ng genomic DNA or 2 μl 1:10-diluted cultured strains were used as the template and mixed with 0.4 μM RF-specific or IF-specific primers and a polymerase mixture from the Qiagen HotStarTaq Master Mix Kit using a three-step cycling program consisting of initial denaturation at 95°C for 15 min; 30 cycles of a three-step procedure including denaturation at 94°C for 30s, annealing at 52°C for 30s, and extension at 72°C for 30s; and a final extension at 72°C for 5 min.

    Article Title: Quantitative trait locus analysis for hemostasis and thrombosis
    Article Snippet: .. PCR was performed using HotstarTaq Master Mix Kit (Qiagen, Valencia, CA). .. The PCR products were detected by electrophoresis on 10% polyacrylamide gel (National Diagnostics, Atlanta, GA) or on 1.5% agarose gel after digestion with restriction endonucleases (New England Biolabs, Beverly, MA) and visualized by ethidium bromide staining.

    Article Title: FGF–2 is required to prevent astrogliosis in the facial nucleus after facial nerve injury and mechanical stimulation of denervated vibrissal muscles
    Article Snippet: .. Genotyping was performed by PCR (Cycling conditions: 30 minutes 95°C, 30 cycles: 30 seconds 95°C, 30 seconds 61°C, 90 seconds 72°C, final elongation for 10 minutes 72°C) using the HotStarTaq Master Mix Kit (Qiagen) from mouse tail DNA and subsequent agarosegel-electrophoresis. .. To differentiate between WT and transgenes, we used the following primers: - neo6: 5′-GAT CTG GAC GAA GAG CAT CAG GGG-3′, - wt6: 5′-CAA GTT TCT AAC TTT CTC CGC TCC TGC-3′, and - wt5: 5ʹ-CAA TCT ATT GGG GTC AAG CCT ATT GGG-3′.

    Article Title: Cysteine Peptidases, Secreted by Trichomonas gallinae, Are Involved in the Cytopathogenic Effects on a Permanent Chicken Liver Cell Culture
    Article Snippet: .. Hot start procedures were used for PCR amplification using the “HotStarTaq Master Mix Kit” (Qiagen, Vienna, Austria). .. PCR was carried out in a 25-μl reaction mixture by using 100ng of trichomonad DNA and each of the primers at 1µM final concentration.

    Article Title: Concurrent genotyping of Helicobacter pylori virulence genes and human cytokine SNP sites using whole genome amplified DNA derived from minute amounts of gastric biopsy specimen DNA
    Article Snippet: .. For that purpose, broad-range 16S rDNA PCR amplicons were purified using a GFX PCR DNA and Gel-band purification kit (GE Healthcare, Uppsala, Sweden) and pyrosequencing PCR amplicons were generated using an appropriate amount (usually 1 μl) of 16S rDNA template, a HotStarTaq Master mix kit (Qiagen AB, Solna, Sweden), 5 pmol each of Helicobacter -specific primer 5'-biotin HJ-HP-JBS.V3 and broad-range primer B-V3.AS (Table ), both flanking the 16S rDNA V3 region only, and PCR condition No 1 (Table ). .. Pyrosequencing analysis was carried out as described elsewhere [ ] using a PSQ 96 MA System (Biotage AB, Uppsala, Sweden) and sequencing primer B-V3.AS (Table ).

    Article Title: KLK10 exon 3 unmethylated PCR product concentration: a new potential early diagnostic marker in ovarian cancer? - A pilot study
    Article Snippet: Commercially available HotStarTaq Master Mix kit (Qiagen) was used. .. Methylated- and unmethylated-specific PCRs were run in parallel in separate PCR tubes where each tube contained either methylated- or unmethylated-specific primer pair, respectively.

    Article Title: A novel RHCE*ce 48C, 733G allele with nucleotide 941C in exon 7, encodes an altered red cell e antigen
    Article Snippet: .. Five microliters of DNA per reaction were amplified by 5U Taq DNA polymerase (HotStarTaq Master Mix Kit; Qiagen) in a 50 μL final reaction mixture containing 3.0 mM MgCl2 , 1X PCR buffer, 0.2 mM dNTPs, and 100 ng of forward and reverse primer. .. Amplification was achieved over 35 cycles with an annealing temperature of 58°C and a final extension time of 10min.

    Article Title: Attachment of oligonucleotide probes to poly carbodiimide-coated glass for microarray applications
    Article Snippet: .. PCR was performed on a mixture of 100 ng of human genome DNA (Roche), 50 pmol of non-fluorescently-labeled forward primer, 50 pmol of non-fluorescently-labeled reverse primer and 25 µl of HotStarTaq Master Mix kit (Qiagen) in a total volume of 50 µl. .. Finally, the PCR mix was incubated at 72°C for 3 min. Amplification of bacteriophage lambda DNA for reuse of the array .

    Article Title: The X chromosome is necessary for ovule production in Silene latifolia
    Article Snippet: .. All PCR reactions were in a 20 μl reaction volume and used HotStarTaq Master Mix Kit (Qiagen) with final primer concentration 2 μM and 1.5 mM MgCl2 . ..

    Article Title: Progastrin-releasing peptide and gastrin-releasing peptide receptor mRNA expression in non-tumor tissues of the human gastrointestinal tract
    Article Snippet: .. PCR was performed using a HotStarTaq Master mix kit (Qiagen, Hilden, Germany) in a final reaction volume of 25 μL. ..

    Article Title: The 6-Kilodalton Early Secreted Antigenic Target-Responsive, Asymptomatic Contacts of Tuberculosis Patients Express Elevated Levels of Interleukin-4 and Reduced Levels of Gamma Interferon
    Article Snippet: .. PCR was carried out in a total volume of 50 μl with 1 μg of cDNA, using the HotStarTaq Master Mix kit (QIAGEN, Dusseldorf, Germany) according to the manufacturer's instructions. .. Primers were designed to span introns so that amplification from genomic DNA should not occur, and this was confirmed by comparing the results from PCR of RNA preparations and the cDNA that was prepared from it.

    Molecular Weight:

    Article Title: The X chromosome is necessary for ovule production in Silene latifolia
    Article Snippet: All PCR reactions were in a 20 μl reaction volume and used HotStarTaq Master Mix Kit (Qiagen) with final primer concentration 2 μM and 1.5 mM MgCl2 . .. Some females also amplified higher molecular weight products with the Sl4RomR and Sl4RomF primers which were never seen in males ( ).

    DNA Extraction:

    Article Title: A novel RHCE*ce 48C, 733G allele with nucleotide 941C in exon 7, encodes an altered red cell e antigen
    Article Snippet: Genomic DNA was prepared from 200 μL of the buffy coat layer of peripheral blood using a DNA extraction kit (QIAamp DNA Blood Mini Kit, Qiagen, Valenica, CA, USA). .. Five microliters of DNA per reaction were amplified by 5U Taq DNA polymerase (HotStarTaq Master Mix Kit; Qiagen) in a 50 μL final reaction mixture containing 3.0 mM MgCl2 , 1X PCR buffer, 0.2 mM dNTPs, and 100 ng of forward and reverse primer.

    Methylation:

    Article Title: KLK10 exon 3 unmethylated PCR product concentration: a new potential early diagnostic marker in ovarian cancer? - A pilot study
    Article Snippet: Commercially available HotStarTaq Master Mix kit (Qiagen) was used. .. Methylated- and unmethylated-specific PCRs were run in parallel in separate PCR tubes where each tube contained either methylated- or unmethylated-specific primer pair, respectively.

    Isolation:

    Article Title: Neural stem cells express melatonin receptors and neurotrophic factors: colocalization of the MT1 receptor with neuronal and glial markers
    Article Snippet: RT-PCR Total RNA was isolated from C17.2 cells with TRIzol as described by the supplier (Invitrogen Canada Inc., Burlington, ON). .. PCR was carried out using 1.5 μl (or 3 μl for melatonin MT1 and MT2 receptors) of the RT product and the HotStarTaq master mix kit (Qiagen Inc., Mississauga, ON), together with appropriate primers (Table ).

    Multiplex Assay:

    Article Title: The molecular function and clinical phenotype of partial deletions of the IGF2/H19 imprinting control region depends on the spatial arrangement of the remaining CTCF-binding sites
    Article Snippet: First, PCR on bisulfite treated DNA was performed with tagged primers using the Qiagen HotStarTaq Master Mix Kit (Qiagen, Hilden, Germany) and standard protocols. .. Sample-specific barcode sequences [(MIDs) multiplex identifiers] and universal linker tags (454 adaptor sequences, A- or B-primer and key) were added in a second PCR conducted as follows: 10 min denaturation at 95°C, then 35 cycles with 95°C for 20 s and 72°C for 30 s and a final elongation step for 7 min at 72°C.

    Mouse Assay:

    Article Title: Quantitative trait locus analysis for hemostasis and thrombosis
    Article Snippet: Genomic DNA was prepared from ear punches of the mice and genotyping was performed using polymerase chain reaction (PCR) for microsatellite markers (Mouse Mappairs, Invitrogen, Carlsbad, CA) and primers for restriction fragment length polymorphism (RFLP) markers (Operon, Huntsville, AL). .. PCR was performed using HotstarTaq Master Mix Kit (Qiagen, Valencia, CA).

    Article Title: FGF–2 is required to prevent astrogliosis in the facial nucleus after facial nerve injury and mechanical stimulation of denervated vibrissal muscles
    Article Snippet: Animals and groups Homozygous mice constitutively lacking all iso- forms of FGF-2, strain Fgf2tm1Zllr C57/Bl6 were used as well as WT animals (C57/Bl6). .. Genotyping was performed by PCR (Cycling conditions: 30 minutes 95°C, 30 cycles: 30 seconds 95°C, 30 seconds 61°C, 90 seconds 72°C, final elongation for 10 minutes 72°C) using the HotStarTaq Master Mix Kit (Qiagen) from mouse tail DNA and subsequent agarosegel-electrophoresis.

    Sequencing:

    Article Title: Gene expression profile of the nucleus accumbens of human cocaine abusers: evidence for dysregulation of myelin
    Article Snippet: RT was performed (Sensiscript RT Kit, Qiagen) using random hexamers while the PCR used sequence-specific primers (amplicons: MBP 70–126, PLP 573–635, MOBP 164–215, CART 189–237 and β-actin 2366–2631). .. Because of the lower basal transcript abundance and greater inducibility of CART, 15 ng input RNA was used for the RT reaction, PCR was performed using the Qiagen HotStarTaq Master Mix Kit, and transcript abundance was quantified using an Agilent DNA 500 LabChip Kit on the Agilent Bioanalyzer 2100.

    Article Title: High Frequency of a Novel Filamentous Phage, VCY?, within an Environmental Vibrio cholerae Population
    Article Snippet: Because different strains were used for sequencing and electron microscopy (EM) of phage and to ensure that the RF and IF are similar phages, we devised specific PCR primers targeting a gene (open reading frame 9 [ORF9]) currently unique to VCYϕ ( ). .. In a typical reaction mixture, 20 ng genomic DNA or 2 μl 1:10-diluted cultured strains were used as the template and mixed with 0.4 μM RF-specific or IF-specific primers and a polymerase mixture from the Qiagen HotStarTaq Master Mix Kit using a three-step cycling program consisting of initial denaturation at 95°C for 15 min; 30 cycles of a three-step procedure including denaturation at 94°C for 30s, annealing at 52°C for 30s, and extension at 72°C for 30s; and a final extension at 72°C for 5 min.

    Article Title: Cysteine Peptidases, Secreted by Trichomonas gallinae, Are Involved in the Cytopathogenic Effects on a Permanent Chicken Liver Cell Culture
    Article Snippet: Paragraph title: PCR amplification and sequence analysis ... Hot start procedures were used for PCR amplification using the “HotStarTaq Master Mix Kit” (Qiagen, Vienna, Austria).

    Article Title: Concurrent genotyping of Helicobacter pylori virulence genes and human cytokine SNP sites using whole genome amplified DNA derived from minute amounts of gastric biopsy specimen DNA
    Article Snippet: For that purpose, broad-range 16S rDNA PCR amplicons were purified using a GFX PCR DNA and Gel-band purification kit (GE Healthcare, Uppsala, Sweden) and pyrosequencing PCR amplicons were generated using an appropriate amount (usually 1 μl) of 16S rDNA template, a HotStarTaq Master mix kit (Qiagen AB, Solna, Sweden), 5 pmol each of Helicobacter -specific primer 5'-biotin HJ-HP-JBS.V3 and broad-range primer B-V3.AS (Table ), both flanking the 16S rDNA V3 region only, and PCR condition No 1 (Table ). .. Pyrosequencing analysis was carried out as described elsewhere [ ] using a PSQ 96 MA System (Biotage AB, Uppsala, Sweden) and sequencing primer B-V3.AS (Table ).

    Article Title: A novel RHCE*ce 48C, 733G allele with nucleotide 941C in exon 7, encodes an altered red cell e antigen
    Article Snippet: Five microliters of DNA per reaction were amplified by 5U Taq DNA polymerase (HotStarTaq Master Mix Kit; Qiagen) in a 50 μL final reaction mixture containing 3.0 mM MgCl2 , 1X PCR buffer, 0.2 mM dNTPs, and 100 ng of forward and reverse primer. .. The 456 bp amplicon was then treated using ExoSAP-IT (USB, Cleveland, OH) prior to direct sequencing of the amplicon and cloning of the amplicon (GENEWIZ, Inc., South Plainfield, NJ).

    Staining:

    Article Title: Quantitative trait locus analysis for hemostasis and thrombosis
    Article Snippet: PCR was performed using HotstarTaq Master Mix Kit (Qiagen, Valencia, CA). .. The PCR products were detected by electrophoresis on 10% polyacrylamide gel (National Diagnostics, Atlanta, GA) or on 1.5% agarose gel after digestion with restriction endonucleases (New England Biolabs, Beverly, MA) and visualized by ethidium bromide staining.

    Article Title: Cysteine Peptidases, Secreted by Trichomonas gallinae, Are Involved in the Cytopathogenic Effects on a Permanent Chicken Liver Cell Culture
    Article Snippet: Hot start procedures were used for PCR amplification using the “HotStarTaq Master Mix Kit” (Qiagen, Vienna, Austria). .. Amplification products (25 µl) were electrophoresed in a 1.0% Tris acetate-EDTA-agarose gel for 60 minutes at 100 V. The gels were stained with ethidium bromide, visualized under UV light (Bio-Rad Universal Hood II, Bio-Rad Laboratories, California, USA), 900bp fragments were excised and then purified with the QIAquick® Gel Extraction Kit (Qiagen, Vienna, Austria).

    Chloramphenicol Acetyltransferase Assay:

    Article Title: FGF–2 is required to prevent astrogliosis in the facial nucleus after facial nerve injury and mechanical stimulation of denervated vibrissal muscles
    Article Snippet: Genotyping was performed by PCR (Cycling conditions: 30 minutes 95°C, 30 cycles: 30 seconds 95°C, 30 seconds 61°C, 90 seconds 72°C, final elongation for 10 minutes 72°C) using the HotStarTaq Master Mix Kit (Qiagen) from mouse tail DNA and subsequent agarosegel-electrophoresis. .. To differentiate between WT and transgenes, we used the following primers: - neo6: 5′-GAT CTG GAC GAA GAG CAT CAG GGG-3′, - wt6: 5′-CAA GTT TCT AAC TTT CTC CGC TCC TGC-3′, and - wt5: 5ʹ-CAA TCT ATT GGG GTC AAG CCT ATT GGG-3′.

    Purification:

    Article Title: Cysteine Peptidases, Secreted by Trichomonas gallinae, Are Involved in the Cytopathogenic Effects on a Permanent Chicken Liver Cell Culture
    Article Snippet: Hot start procedures were used for PCR amplification using the “HotStarTaq Master Mix Kit” (Qiagen, Vienna, Austria). .. Amplification products (25 µl) were electrophoresed in a 1.0% Tris acetate-EDTA-agarose gel for 60 minutes at 100 V. The gels were stained with ethidium bromide, visualized under UV light (Bio-Rad Universal Hood II, Bio-Rad Laboratories, California, USA), 900bp fragments were excised and then purified with the QIAquick® Gel Extraction Kit (Qiagen, Vienna, Austria).

    Article Title: Concurrent genotyping of Helicobacter pylori virulence genes and human cytokine SNP sites using whole genome amplified DNA derived from minute amounts of gastric biopsy specimen DNA
    Article Snippet: .. For that purpose, broad-range 16S rDNA PCR amplicons were purified using a GFX PCR DNA and Gel-band purification kit (GE Healthcare, Uppsala, Sweden) and pyrosequencing PCR amplicons were generated using an appropriate amount (usually 1 μl) of 16S rDNA template, a HotStarTaq Master mix kit (Qiagen AB, Solna, Sweden), 5 pmol each of Helicobacter -specific primer 5'-biotin HJ-HP-JBS.V3 and broad-range primer B-V3.AS (Table ), both flanking the 16S rDNA V3 region only, and PCR condition No 1 (Table ). .. Pyrosequencing analysis was carried out as described elsewhere [ ] using a PSQ 96 MA System (Biotage AB, Uppsala, Sweden) and sequencing primer B-V3.AS (Table ).

    Article Title: Attachment of oligonucleotide probes to poly carbodiimide-coated glass for microarray applications
    Article Snippet: PCR was performed on a mixture of 100 ng of human genome DNA (Roche), 50 pmol of non-fluorescently-labeled forward primer, 50 pmol of non-fluorescently-labeled reverse primer and 25 µl of HotStarTaq Master Mix kit (Qiagen) in a total volume of 50 µl. .. Finally, the PCR mix was incubated at 72°C for 3 min. Purification and cloning of the PCR products .

    SYBR Green Assay:

    Article Title: Gene expression profile of the nucleus accumbens of human cocaine abusers: evidence for dysregulation of myelin
    Article Snippet: For myelin-related transcripts, PCR was performed in the LightCycler version 3.3 with the Qiagen SYBR Green PCR Kit (Roche, Indianapolis, IN, USA). .. Because of the lower basal transcript abundance and greater inducibility of CART, 15 ng input RNA was used for the RT reaction, PCR was performed using the Qiagen HotStarTaq Master Mix Kit, and transcript abundance was quantified using an Agilent DNA 500 LabChip Kit on the Agilent Bioanalyzer 2100.

    Negative Control:

    Article Title: Progastrin-releasing peptide and gastrin-releasing peptide receptor mRNA expression in non-tumor tissues of the human gastrointestinal tract
    Article Snippet: PCR was performed using a HotStarTaq Master mix kit (Qiagen, Hilden, Germany) in a final reaction volume of 25 μL. .. Quick-clone human pancreas and stomach cDNA (Clontech, BD Biosciences Stockholm, Sweden) were used as positive PCR amplification controls, whereas HotStar PCR amplification mix without ss-cDNA addition was used as a negative control.

    Agarose Gel Electrophoresis:

    Article Title: High Frequency of a Novel Filamentous Phage, VCY?, within an Environmental Vibrio cholerae Population
    Article Snippet: In a typical reaction mixture, 20 ng genomic DNA or 2 μl 1:10-diluted cultured strains were used as the template and mixed with 0.4 μM RF-specific or IF-specific primers and a polymerase mixture from the Qiagen HotStarTaq Master Mix Kit using a three-step cycling program consisting of initial denaturation at 95°C for 15 min; 30 cycles of a three-step procedure including denaturation at 94°C for 30s, annealing at 52°C for 30s, and extension at 72°C for 30s; and a final extension at 72°C for 5 min. .. The PCR products were separated on a 1.8% agarose gel prepared with 0.5× Tris-borate-EDTA buffer.

    Article Title: Quantitative trait locus analysis for hemostasis and thrombosis
    Article Snippet: PCR was performed using HotstarTaq Master Mix Kit (Qiagen, Valencia, CA). .. The PCR products were detected by electrophoresis on 10% polyacrylamide gel (National Diagnostics, Atlanta, GA) or on 1.5% agarose gel after digestion with restriction endonucleases (New England Biolabs, Beverly, MA) and visualized by ethidium bromide staining.

    Microarray:

    Article Title: Gene expression profile of the nucleus accumbens of human cocaine abusers: evidence for dysregulation of myelin
    Article Snippet: RNA from all 20 subjects was used for verification of the microarray data. .. Because of the lower basal transcript abundance and greater inducibility of CART, 15 ng input RNA was used for the RT reaction, PCR was performed using the Qiagen HotStarTaq Master Mix Kit, and transcript abundance was quantified using an Agilent DNA 500 LabChip Kit on the Agilent Bioanalyzer 2100.

    Spectrophotometry:

    Article Title: The 6-Kilodalton Early Secreted Antigenic Target-Responsive, Asymptomatic Contacts of Tuberculosis Patients Express Elevated Levels of Interleukin-4 and Reduced Levels of Gamma Interferon
    Article Snippet: The mRNA was transcribed into cDNA, using the Omniscript reverse transcription kit (QIAGEN, Dusseldorf, Germany) with oligo(dT) primers according to the manufacturer's instructions; the concentration was calculated from the optical density using a GeneQuant spectrophotometer (Amersham Biosciences, Amersham, United Kingdom); and the sample was stored at −20°C until use. .. PCR was carried out in a total volume of 50 μl with 1 μg of cDNA, using the HotStarTaq Master Mix kit (QIAGEN, Dusseldorf, Germany) according to the manufacturer's instructions.

    Concentration Assay:

    Article Title: Cysteine Peptidases, Secreted by Trichomonas gallinae, Are Involved in the Cytopathogenic Effects on a Permanent Chicken Liver Cell Culture
    Article Snippet: Hot start procedures were used for PCR amplification using the “HotStarTaq Master Mix Kit” (Qiagen, Vienna, Austria). .. PCR was carried out in a 25-μl reaction mixture by using 100ng of trichomonad DNA and each of the primers at 1µM final concentration.

    Article Title: The X chromosome is necessary for ovule production in Silene latifolia
    Article Snippet: .. All PCR reactions were in a 20 μl reaction volume and used HotStarTaq Master Mix Kit (Qiagen) with final primer concentration 2 μM and 1.5 mM MgCl2 . ..

    Article Title: The 6-Kilodalton Early Secreted Antigenic Target-Responsive, Asymptomatic Contacts of Tuberculosis Patients Express Elevated Levels of Interleukin-4 and Reduced Levels of Gamma Interferon
    Article Snippet: The mRNA was transcribed into cDNA, using the Omniscript reverse transcription kit (QIAGEN, Dusseldorf, Germany) with oligo(dT) primers according to the manufacturer's instructions; the concentration was calculated from the optical density using a GeneQuant spectrophotometer (Amersham Biosciences, Amersham, United Kingdom); and the sample was stored at −20°C until use. .. PCR was carried out in a total volume of 50 μl with 1 μg of cDNA, using the HotStarTaq Master Mix kit (QIAGEN, Dusseldorf, Germany) according to the manufacturer's instructions.

    CTG Assay:

    Article Title: FGF–2 is required to prevent astrogliosis in the facial nucleus after facial nerve injury and mechanical stimulation of denervated vibrissal muscles
    Article Snippet: Genotyping was performed by PCR (Cycling conditions: 30 minutes 95°C, 30 cycles: 30 seconds 95°C, 30 seconds 61°C, 90 seconds 72°C, final elongation for 10 minutes 72°C) using the HotStarTaq Master Mix Kit (Qiagen) from mouse tail DNA and subsequent agarosegel-electrophoresis. .. To differentiate between WT and transgenes, we used the following primers: - neo6: 5′-GAT CTG GAC GAA GAG CAT CAG GGG-3′, - wt6: 5′-CAA GTT TCT AAC TTT CTC CGC TCC TGC-3′, and - wt5: 5ʹ-CAA TCT ATT GGG GTC AAG CCT ATT GGG-3′.

    Gel Extraction:

    Article Title: Cysteine Peptidases, Secreted by Trichomonas gallinae, Are Involved in the Cytopathogenic Effects on a Permanent Chicken Liver Cell Culture
    Article Snippet: Hot start procedures were used for PCR amplification using the “HotStarTaq Master Mix Kit” (Qiagen, Vienna, Austria). .. Amplification products (25 µl) were electrophoresed in a 1.0% Tris acetate-EDTA-agarose gel for 60 minutes at 100 V. The gels were stained with ethidium bromide, visualized under UV light (Bio-Rad Universal Hood II, Bio-Rad Laboratories, California, USA), 900bp fragments were excised and then purified with the QIAquick® Gel Extraction Kit (Qiagen, Vienna, Austria).

    Hood:

    Article Title: Cysteine Peptidases, Secreted by Trichomonas gallinae, Are Involved in the Cytopathogenic Effects on a Permanent Chicken Liver Cell Culture
    Article Snippet: Hot start procedures were used for PCR amplification using the “HotStarTaq Master Mix Kit” (Qiagen, Vienna, Austria). .. Amplification products (25 µl) were electrophoresed in a 1.0% Tris acetate-EDTA-agarose gel for 60 minutes at 100 V. The gels were stained with ethidium bromide, visualized under UV light (Bio-Rad Universal Hood II, Bio-Rad Laboratories, California, USA), 900bp fragments were excised and then purified with the QIAquick® Gel Extraction Kit (Qiagen, Vienna, Austria).

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    Qiagen hotstartaq master mix kit
    Hotstartaq Master Mix Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 263 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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