phosphatase inhibitor mixtures  (Millipore)


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    Structured Review

    Millipore phosphatase inhibitor mixtures
    Phosphatase Inhibitor Mixtures, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 1536 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phosphatase inhibitor mixtures/product/Millipore
    Average 92 stars, based on 1536 article reviews
    Price from $9.99 to $1999.99
    phosphatase inhibitor mixtures - by Bioz Stars, 2020-09
    92/100 stars

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    Isolation:

    Article Title: Functional interaction of human Ssu72 with RNA polymerase II complexes
    Article Snippet: .. If RNA was to be isolated, elongation complexes were stopped with stop solution containing 100 mM Tris pH 7.6, 0.2 mg/ml Torula Yeast RNA, 20 mM EDTA, and 1% Sarkosyl (Sigma, R6625). .. The RNA was then isolated by phenol extraction and precipitated by addition of 3 volumes of 95% ethanol containing 0.5 M ammonium acetate.

    Incubation:

    Article Title: Domain Stability in the AAA+ ATPase ClpB from Escherichia coli a
    Article Snippet: .. ClpB samples were incubated for 15 min at 37 °C in 100 mM Tris-HCl pH 8, 10 mM MgCl2 , 5 mM ATP, 1 mM EDTA, 1 mM DTT without or with 0.1 mg/ml κ-casein (Sigma). ..

    Purification:

    Article Title: Lyso-Phospholipid Micelles Sustain the Stability and Catalytic Activity of Diacylglycerol Kinase in the Absence of Lipids †
    Article Snippet: .. When the protein was purified into LMPC and DPC, D2 O and EDTA were added to 10% and 0.5 mM, respectively, and the sample was concentrated using centrifugal ultrafiltration (Millipore Ultracel, 10 ml, 10kDa cutoff) and the sample was transferred to an NMR tube. .. For the protein in TDPC and LMPG the pH 7.8 purification buffer containing 250 mM imidazole (pH 7.8) was exchanged for a pH 6.5 10 mM Bis-Tris buffer by repeated centrifugal ultrafiltration/redilution cycles.

    Nuclear Magnetic Resonance:

    Article Title: Lyso-Phospholipid Micelles Sustain the Stability and Catalytic Activity of Diacylglycerol Kinase in the Absence of Lipids †
    Article Snippet: .. When the protein was purified into LMPC and DPC, D2 O and EDTA were added to 10% and 0.5 mM, respectively, and the sample was concentrated using centrifugal ultrafiltration (Millipore Ultracel, 10 ml, 10kDa cutoff) and the sample was transferred to an NMR tube. .. For the protein in TDPC and LMPG the pH 7.8 purification buffer containing 250 mM imidazole (pH 7.8) was exchanged for a pH 6.5 10 mM Bis-Tris buffer by repeated centrifugal ultrafiltration/redilution cycles.

    other:

    Article Title: Novel Methylselenoesters as Antiproliferative Agents
    Article Snippet: A mixture of 0.1 M Tris pH 8, 2 mM EDTA pH = 8, 0.1 mg/mL BSA, 1 mM GSH, 200 μM NADPH, and 0.008 OD/mL yeast GR was prepared (all reagents were purchased from Sigma-Aldrich).

    Article Title: A lipocalin mediates unidirectional haem biomineralization in malaria parasites
    Article Snippet: Membrane pellets were resuspended in 0.1 M Na2CO3 in PBS or in 1% Triton X-100 in PBS, respectively, and spun 50 minutes at 100,000 ×g .

    Article Title: The 20S proteasome activator PA28γ controls the compaction of chromatin
    Article Snippet: In some specific experiments, cells were permeabilized prior the fixation with 0.5% Triton X-100 in PBS for 5 min at 4°C for the colocalization between endogenous PA28γ and HP1 β in U2OS cells.

    Protease Inhibitor:

    Article Title: Estrogen receptor α dependent regulation of estrogen related receptor β and its role in cell cycle in breast cancer
    Article Snippet: .. Cells were lysed in SDS lysis buffer (1% ( w / v ) SDS, 10 mM EDTA, 50 mM Tris-HCl (pH 8.1)) with protease inhibitor cocktail (Sigma-Aldrich) and were sonicated using Bioruptor ultrasonicator device (Diagenode S.A., Seraing, Belgium) at M2 amplitude strength. .. The sonicated samples were subjected to pre-clearing with protein A/G agarose beads (GE Healthcare Life Sciences).

    Sonication:

    Article Title: Estrogen receptor α dependent regulation of estrogen related receptor β and its role in cell cycle in breast cancer
    Article Snippet: .. Cells were lysed in SDS lysis buffer (1% ( w / v ) SDS, 10 mM EDTA, 50 mM Tris-HCl (pH 8.1)) with protease inhibitor cocktail (Sigma-Aldrich) and were sonicated using Bioruptor ultrasonicator device (Diagenode S.A., Seraing, Belgium) at M2 amplitude strength. .. The sonicated samples were subjected to pre-clearing with protein A/G agarose beads (GE Healthcare Life Sciences).

    Lysis:

    Article Title: Estrogen receptor α dependent regulation of estrogen related receptor β and its role in cell cycle in breast cancer
    Article Snippet: .. Cells were lysed in SDS lysis buffer (1% ( w / v ) SDS, 10 mM EDTA, 50 mM Tris-HCl (pH 8.1)) with protease inhibitor cocktail (Sigma-Aldrich) and were sonicated using Bioruptor ultrasonicator device (Diagenode S.A., Seraing, Belgium) at M2 amplitude strength. .. The sonicated samples were subjected to pre-clearing with protein A/G agarose beads (GE Healthcare Life Sciences).

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    Millipore 200 ãŽâ¼m na3 vo4
    200 ãŽâ¼m Na3 Vo4, supplied by Millipore, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/200 ãŽâ¼m na3 vo4/product/Millipore
    Average 84 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    200 ãŽâ¼m na3 vo4 - by Bioz Stars, 2020-09
    84/100 stars
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