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TaKaRa 20 amino acid farnesylation signal
20 Amino Acid Farnesylation Signal, supplied by TaKaRa, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/20 amino acid farnesylation signal/product/TaKaRa
Average 79 stars, based on 1 article reviews
Price from $9.99 to $1999.99
20 amino acid farnesylation signal - by Bioz Stars, 2020-02
79/100 stars

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Polymerase Chain Reaction:

Article Title: Non-invasive intravital imaging of cellular differentiation with a bright red-excitable fluorescent protein
Article Snippet: A cDNA fragment encoding each protein domain was PCR amplified with primers containing the appropriate restriction enzyme sites and ligated into pmCardinal-C1 or pmCardinal-N1. .. To prepare C-terminal fusions to mCardinal, the following digests were performed: human β-actin (NM_001101.3, Clontech), NheI and BglII; human α-tubulin (NM_006082, Clontech), NheI and BglII; human Rab4a (NM_004578.2, Viki Allen, University of Manchester, U.K.), BspEI and BamHI; human lamin B1 (NM_005573.2, George Patterson, NIH), EcoRI and BamHI; human myotilin (NM_006790.1, Origene), AgeI and BspEI; human fibrillarin (NM_001436.3, Evrogen), BglII and BamHI; human tight junction protein ZO1 (NM_003257.1, Origene), AgeI and BspEI; human VE cadherin (NM_001795.3, Origene), BglII and EcoRI; and the 20-amino-acid farnesylation signal from c-Ha-Ras (NM_001130442.1, Clontech), AgeI and BspEI.

Clone Assay:

Article Title: Non-invasive intravital imaging of cellular differentiation with a bright red-excitable fluorescent protein
Article Snippet: The purified and digested PCR products were ligated into similarly digested pEGFP-C1 and pEGFP-N1 cloning vectors to create pmCardinal-C1 and pmCardinal-N1. .. To prepare C-terminal fusions to mCardinal, the following digests were performed: human β-actin (NM_001101.3, Clontech), NheI and BglII; human α-tubulin (NM_006082, Clontech), NheI and BglII; human Rab4a (NM_004578.2, Viki Allen, University of Manchester, U.K.), BspEI and BamHI; human lamin B1 (NM_005573.2, George Patterson, NIH), EcoRI and BamHI; human myotilin (NM_006790.1, Origene), AgeI and BspEI; human fibrillarin (NM_001436.3, Evrogen), BglII and BamHI; human tight junction protein ZO1 (NM_003257.1, Origene), AgeI and BspEI; human VE cadherin (NM_001795.3, Origene), BglII and EcoRI; and the 20-amino-acid farnesylation signal from c-Ha-Ras (NM_001130442.1, Clontech), AgeI and BspEI.

Amplification:

Article Title: Non-invasive intravital imaging of cellular differentiation with a bright red-excitable fluorescent protein
Article Snippet: A cDNA fragment encoding each protein domain was PCR amplified with primers containing the appropriate restriction enzyme sites and ligated into pmCardinal-C1 or pmCardinal-N1. .. To prepare C-terminal fusions to mCardinal, the following digests were performed: human β-actin (NM_001101.3, Clontech), NheI and BglII; human α-tubulin (NM_006082, Clontech), NheI and BglII; human Rab4a (NM_004578.2, Viki Allen, University of Manchester, U.K.), BspEI and BamHI; human lamin B1 (NM_005573.2, George Patterson, NIH), EcoRI and BamHI; human myotilin (NM_006790.1, Origene), AgeI and BspEI; human fibrillarin (NM_001436.3, Evrogen), BglII and BamHI; human tight junction protein ZO1 (NM_003257.1, Origene), AgeI and BspEI; human VE cadherin (NM_001795.3, Origene), BglII and EcoRI; and the 20-amino-acid farnesylation signal from c-Ha-Ras (NM_001130442.1, Clontech), AgeI and BspEI.

Purification:

Article Title: Non-invasive intravital imaging of cellular differentiation with a bright red-excitable fluorescent protein
Article Snippet: The purified and digested PCR products were ligated into similarly digested pEGFP-C1 and pEGFP-N1 cloning vectors to create pmCardinal-C1 and pmCardinal-N1. .. To prepare C-terminal fusions to mCardinal, the following digests were performed: human β-actin (NM_001101.3, Clontech), NheI and BglII; human α-tubulin (NM_006082, Clontech), NheI and BglII; human Rab4a (NM_004578.2, Viki Allen, University of Manchester, U.K.), BspEI and BamHI; human lamin B1 (NM_005573.2, George Patterson, NIH), EcoRI and BamHI; human myotilin (NM_006790.1, Origene), AgeI and BspEI; human fibrillarin (NM_001436.3, Evrogen), BglII and BamHI; human tight junction protein ZO1 (NM_003257.1, Origene), AgeI and BspEI; human VE cadherin (NM_001795.3, Origene), BglII and EcoRI; and the 20-amino-acid farnesylation signal from c-Ha-Ras (NM_001130442.1, Clontech), AgeI and BspEI.

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    TaKaRa 20 amino acid farnesylation signal
    20 Amino Acid Farnesylation Signal, supplied by TaKaRa, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/20 amino acid farnesylation signal/product/TaKaRa
    Average 79 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    20 amino acid farnesylation signal - by Bioz Stars, 2020-02
    79/100 stars
      Buy from Supplier

    83
    TaKaRa amino acid aa farnesylation signal
    Amino Acid Aa Farnesylation Signal, supplied by TaKaRa, used in various techniques. Bioz Stars score: 83/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amino acid aa farnesylation signal/product/TaKaRa
    Average 83 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    amino acid aa farnesylation signal - by Bioz Stars, 2020-02
    83/100 stars
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