Structured Review

TaKaRa 2 step pcr strategy
2 Step Pcr Strategy, supplied by TaKaRa, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/2 step pcr strategy/product/TaKaRa
Average 91 stars, based on 1 article reviews
Price from $9.99 to $1999.99
2 step pcr strategy - by Bioz Stars, 2020-07
91/100 stars

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Related Articles

Sequencing:

Article Title: Systematic evaluation of supervised classifiers for fecal microbiota-based prediction of colorectal cancer
Article Snippet: .. The V1∼V3 hypervariable regions of the 16S rRNA gene(27F 5′-AGAGTTTGATCCTGGCTCAG-3′, 533R 5′-TTACCGC GGCTGCTGGCAC-3′) incorporating the FLX Titanium adaptors and a sample barcode sequence from each sample were amplified using a 2-step PCR strategy(Takara Bio Inc).After the PCR reaction, electrophoresis was immediately performed to isolate the enriched V1-V3 region DNA fragments from the reaction mixture. .. All of the products were harvested by using a gel extraction kit (OMEGA Bio-tek) according to the manufacturer's instructions.

Polymerase Chain Reaction:

Article Title: Systematic evaluation of supervised classifiers for fecal microbiota-based prediction of colorectal cancer
Article Snippet: .. The V1∼V3 hypervariable regions of the 16S rRNA gene(27F 5′-AGAGTTTGATCCTGGCTCAG-3′, 533R 5′-TTACCGC GGCTGCTGGCAC-3′) incorporating the FLX Titanium adaptors and a sample barcode sequence from each sample were amplified using a 2-step PCR strategy(Takara Bio Inc).After the PCR reaction, electrophoresis was immediately performed to isolate the enriched V1-V3 region DNA fragments from the reaction mixture. .. All of the products were harvested by using a gel extraction kit (OMEGA Bio-tek) according to the manufacturer's instructions.

Amplification:

Article Title: Systematic evaluation of supervised classifiers for fecal microbiota-based prediction of colorectal cancer
Article Snippet: .. The V1∼V3 hypervariable regions of the 16S rRNA gene(27F 5′-AGAGTTTGATCCTGGCTCAG-3′, 533R 5′-TTACCGC GGCTGCTGGCAC-3′) incorporating the FLX Titanium adaptors and a sample barcode sequence from each sample were amplified using a 2-step PCR strategy(Takara Bio Inc).After the PCR reaction, electrophoresis was immediately performed to isolate the enriched V1-V3 region DNA fragments from the reaction mixture. .. All of the products were harvested by using a gel extraction kit (OMEGA Bio-tek) according to the manufacturer's instructions.

Electrophoresis:

Article Title: Systematic evaluation of supervised classifiers for fecal microbiota-based prediction of colorectal cancer
Article Snippet: .. The V1∼V3 hypervariable regions of the 16S rRNA gene(27F 5′-AGAGTTTGATCCTGGCTCAG-3′, 533R 5′-TTACCGC GGCTGCTGGCAC-3′) incorporating the FLX Titanium adaptors and a sample barcode sequence from each sample were amplified using a 2-step PCR strategy(Takara Bio Inc).After the PCR reaction, electrophoresis was immediately performed to isolate the enriched V1-V3 region DNA fragments from the reaction mixture. .. All of the products were harvested by using a gel extraction kit (OMEGA Bio-tek) according to the manufacturer's instructions.

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    TaKaRa 2 step pcr strategy
    2 Step Pcr Strategy, supplied by TaKaRa, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/2 step pcr strategy/product/TaKaRa
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    2 step pcr strategy - by Bioz Stars, 2020-07
    91/100 stars
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