2 7 difluorofluorescein maleimide  (Thermo Fisher)


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  • 93
    Name:
    2 7 Difluorofluorescein Oregon Green 488
    Description:
    Oregon Green 488 can be used as a reference standard for Oregon Green 488 conjugates or a pH sensor of moderately acidic solutions pKa 4 7
    Catalog Number:
    d6145
    Price:
    None
    Applications:
    Amine-Reactive Fluorophores|Cell Analysis|Labeling Amines|Protein Biology|Protein Labeling|Protein Labeling & Crosslinking|Protein and Antibody Labeling|Labeling Chemistry
    Category:
    Labeling Detection Products
    Buy from Supplier


    Structured Review

    Thermo Fisher 2 7 difluorofluorescein maleimide
    Oregon Green 488 can be used as a reference standard for Oregon Green 488 conjugates or a pH sensor of moderately acidic solutions pKa 4 7
    https://www.bioz.com/result/2 7 difluorofluorescein maleimide/product/Thermo Fisher
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    2 7 difluorofluorescein maleimide - by Bioz Stars, 2020-08
    93/100 stars

    Related Products / Commonly Used Together

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    Related Articles

    IA:

    Article Title: Secretory ribonucleases are internalized by a dynamin-independent endocytic pathway
    Article Snippet: .. The fluorescent probes 5-iodoacetamidofluorescein (5-IAF), N-(4,4-difluoro-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-s-indacene-2-yl)iodoacetamide (BODIPY® 507/545 IA), and 2′,7′-difluorofluorescein-iodoacetamide (Oregon Green™ 488 IA) were from Molecular Probes (Eugene, OR). .. Excess, unreacted probe was removed by gel filtration chromatography with a NICK column (Pharmacia Biotech; Piscataway, NJ).

    other:

    Article Title: Three-level spaser for next-generation luminescent nanoprobe
    Article Snippet: Under continuous stirring, 10 μl of 3-mercaptopropyltrimethoxysilane (Sigma-Aldrich)–bonded 2′,7′-difluorofluorescein maleimide (4.56 mM; Invitrogen), 1 μl of tetraethoxysilane, and 2 μl of ammonium hydroxide were added to the above solution ( ).

    Article Title: TCA cycle inactivation in Staphylococcus aureus alters nitric oxide production in RAW 264.7 cells
    Article Snippet: To determine intracellular production of ROS and NO• in RAW 264.7 cells exposed to bacteria, 5-(and-6)-chloromethyl-2′,7′-dichlorodihydrofluorescein diacetate acetyl ester (CM-H2 DCFDA) and 4-amino-5-methylamino-2′, 7′-difluorofluorescein (DAF-FM; Invitrogen, Eugene, CA) were used as ROS and NO• indicators, respectively [ – ].

    Article Title: P2X7 Receptor Activation Induces Reactive Oxygen Species Formation and Cell Death in Murine EOC13 Microglia
    Article Snippet: Reagents and Antibodies RPMI-1640 and DMEM/F12 media, GlutaMAX, normal horse serum (NHS), 0.05% trypsin, YO-PRO-12+ , 2′,7′-dichlorodihydrofluorescein diacetate (H2 DCFDA), and 2′,7′-difluorofluorescein diacetate (DAF-FM DA) were from Invitrogen (Grand Island, NY).

    Article Title: Contact-free determination of viscosity in multiple parallel samples
    Article Snippet: Oregon Green® 488 (D6145, Molecular Probes, Inc., Eugene, OR, USA) is a fluorinated analog of fluorescein exhibiting the same excitation and emission wavelength and was used as an alternative fluorescent dye.

    Cell Culture:

    Article Title: Increasing TRPV4 expression restores flow-induced dilation impaired in mesenteric arteries with aging
    Article Snippet: .. Measurement of NO production in primary cultured MAECs MAECs seeded on circular coverslips were loaded with 2 μM 4-amino-5-methylamino-2′, 7′-difluorofluorescein (DAF-FM DA; Molecular Probes) at room temperature for 10 min. .. The fluorescence intensity excited at 495 nm and emitted at 515 nm was determined using a Leica TCS SP5 confocal laser system.

    Sampling:

    Article Title: Utilizing transmembrane convection to enhance solute sampling and delivery by microdialysis: theory and in vitro validation
    Article Snippet: .. In an experiment to concurrently measure extraction fractions for sampling and delivery, probes were perfused with a solution containing difluorofluorescein (2FF, D6145) while immersed in a solution of fluorescein (FLR, F1300), both dyes purchased from Molecular Probes, Eugene, OR. .. The dialysate samples were analyzed for both compounds using HPLC with fluorescence detection and the concentration-based extraction fractions were calculated from as E C w s [ 2 FF loss ] = C i n − C out C i n and E C w s [ FLR gain ] = C out C e ∞ . (80) The corresponding mass-based extraction fractions, E M w s , were calculated from .