m nacl  (Thermo Fisher)


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  • 99
    Name:
    NaCl 5 M
    Description:
    Ambion Molecular biology grade 5 M NaCl solution is supplied in one bottle containing 500 mL The solution is certified RNase free economical and ready to use Due to the ubiquitous presence of RNases manufacturing products for use with RNA is especially challenging Ambion s nuclease free reagents and buffers are manufactured in facilities specifically designed to prevent the introduction of nucleases Highly sensitive RNase assays are performed at several different stages of the manufacturing process to ensure the highest quality These reagents are rigorously tested for contaminating nonspecific endonuclease exonuclease and RNase activity
    Catalog Number:
    am9759
    Price:
    None
    Category:
    Lab Reagents and Chemicals
    Buy from Supplier


    Structured Review

    Thermo Fisher m nacl
    Ambion Molecular biology grade 5 M NaCl solution is supplied in one bottle containing 500 mL The solution is certified RNase free economical and ready to use Due to the ubiquitous presence of RNases manufacturing products for use with RNA is especially challenging Ambion s nuclease free reagents and buffers are manufactured in facilities specifically designed to prevent the introduction of nucleases Highly sensitive RNase assays are performed at several different stages of the manufacturing process to ensure the highest quality These reagents are rigorously tested for contaminating nonspecific endonuclease exonuclease and RNase activity
    https://www.bioz.com/result/m nacl/product/Thermo Fisher
    Average 99 stars, based on 392 article reviews
    Price from $9.99 to $1999.99
    m nacl - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Amplification:

    Article Title: High NaCl-induced inhibition of PTG contributes to activation of NFAT5 through attenuation of the negative effect of SHP-1
    Article Snippet: .. HEK293 cells were treated with high NaCl or replaced by fresh control medium for 16 h in a 96-well plate, and then cells were lysed and cDNAs were synthesized using the Cells-to-Ct kit (Applied Biosystems) and amplified by Taqman 7900HT (Applied Biosystems). ..

    Chromatography:

    Article Title: Ultracentrifugation-free chromatography-mediated large-scale purification of recombinant adeno-associated virus serotype 1 (rAAV1)
    Article Snippet: .. After 1/3→1/2 AS, the sample was dissolved in 3.3 mmol/l morpholinoethanesulfonic acid, 3.3 mmol/l HEPES, and 3.3 mmol/l sodium acetate (MHN) buffer (pH 6.5) containing 50 mmol/l NaCl and 0.01% Pluronic F-68 (Gibco, Invitrogen, Grand Island, NY) and was loaded onto the dual ion-exchange column configuration (cationic: Mustang SXT; anionic: Mustang QXT, Pall Corporation, Ann Arbor, MI) chromatography. ..

    Synthesized:

    Article Title: High NaCl-induced inhibition of PTG contributes to activation of NFAT5 through attenuation of the negative effect of SHP-1
    Article Snippet: .. HEK293 cells were treated with high NaCl or replaced by fresh control medium for 16 h in a 96-well plate, and then cells were lysed and cDNAs were synthesized using the Cells-to-Ct kit (Applied Biosystems) and amplified by Taqman 7900HT (Applied Biosystems). ..

    Cell Culture:

    Article Title: Calcineurin and Calcium Channel CchA Coordinate the Salt Stress Response by Regulating Cytoplasmic Ca2+ Homeostasis in Aspergillus nidulans
    Article Snippet: .. A total of 1 × 106 spores were cultured on either MMPDRUU or MMPDRUU with 800 mM NaCl in a 96-well microtiter plate (Thermo Fischer, United Kingdom) and then incubated at 37°C for 18 h. The medium was then removed, and the mycelia were rinsed twice with PGM: 20 mM PIPES [piperazine- N , N ′-bis(2-ethanesulfonic acid)] (pH 6.7), 50 mM glucose, and 1 mM MgCl2 . .. Aequorin was reconstituted by incubation of mycelia in 100 μl PGM containing 2.5 μM coelenterazine for 4 h at 4°C in the dark.

    Incubation:

    Article Title: Calcineurin and Calcium Channel CchA Coordinate the Salt Stress Response by Regulating Cytoplasmic Ca2+ Homeostasis in Aspergillus nidulans
    Article Snippet: .. A total of 1 × 106 spores were cultured on either MMPDRUU or MMPDRUU with 800 mM NaCl in a 96-well microtiter plate (Thermo Fischer, United Kingdom) and then incubated at 37°C for 18 h. The medium was then removed, and the mycelia were rinsed twice with PGM: 20 mM PIPES [piperazine- N , N ′-bis(2-ethanesulfonic acid)] (pH 6.7), 50 mM glucose, and 1 mM MgCl2 . .. Aequorin was reconstituted by incubation of mycelia in 100 μl PGM containing 2.5 μM coelenterazine for 4 h at 4°C in the dark.

    Sonication:

    Article Title: Interaction between the H2 Sensor HupUV and the Histidine Kinase HupT Controls HupSL Hydrogenase Synthesis in Rhodobacter capsulatus
    Article Snippet: .. The cells were resuspended in 75 ml of ice-cold IMAC-5 (20 mM Tris-HCl [pH 8], 0.5 mM NaCl, 10% glycerol, 5 mM imidazole) containing 1 mM phenylmethylsulfonyl fluoride, and disrupted by passage twice through a French press cell (Thermo Spectronic) at 15,000 lb/in2 (followed by 30 s of sonication). .. The cytosolic fraction was then isolated by centrifugation at 200,000 × g twice during 1 h at 4°C in a 60 Ti rotor (Beckman Corp.).

    SDS Page:

    Article Title: Monitoring Intracellular pH change with a Genetically Encoded and Ratiometric Luminescence Sensor in Yeast and Mammalian Cells
    Article Snippet: .. 50-mL flask and 2-L flask LB medium with ampicillin (60 μg/mL) TALON metal affinity resin (Clontech) Equilibration/Wash buffer: 50 mM sodium phosphate, 300 mM NaCl, pH 7.0 Elution buffer: 50 mM sodium phosphate, 300 mM NaCl, 150 mM imidazole, pH 7.0 10% SDS-PAGE gel Dialysis cassette (20K MWCO) (Thermo Scientific) Dialysis buffer: 30 mM MOPS, 5% Glycerol, pH 7.2 BSA standard solution and Protein Assay Dye Reagent Concentrate (Bio-Rad) ..