1x t4 dna polymerase buffer  (Thermo Fisher)


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    Name:
    T4 DNA Polymerase 5 U µL
    Description:
    Thermo Scientific T4 DNA Polymerase is a template dependent DNA polymerase that catalyzes 5 3 synthesis from primed single stranded DNA The enzyme has a 3 5 exonuclease activity but lacks 5 3 exonuclease activity Highlights• Stronger 3 5 exonuclease activity on single stranded than on double stranded DNA and greater more than 200 times than DNA polymerase I E coli and Klenow fragment• Active in Thermo Scientific restriction enzyme PCR RT and T4 DNA Ligase buffersApplications• Blunting of DNA ends fill in of 5 overhangs or and removal of 3 overhangs see​ References1 2 • Blunting of PCR products with 3 dA overhangs• Synthesis of labeled DNA probes by the replacement reaction see​ Reference3 • Oligonucleotide directed site specific mutagenesis see​ Reference4 • Ligation independent cloning of PCR products
    Catalog Number:
    ep0061
    Price:
    None
    Applications:
    Cloning|PCR Cloning|Restriction Enzyme Cloning|Mutagenesis
    Category:
    Proteins Enzymes Peptides
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    Structured Review

    Thermo Fisher 1x t4 dna polymerase buffer
    Thermo Scientific T4 DNA Polymerase is a template dependent DNA polymerase that catalyzes 5 3 synthesis from primed single stranded DNA The enzyme has a 3 5 exonuclease activity but lacks 5 3 exonuclease activity Highlights• Stronger 3 5 exonuclease activity on single stranded than on double stranded DNA and greater more than 200 times than DNA polymerase I E coli and Klenow fragment• Active in Thermo Scientific restriction enzyme PCR RT and T4 DNA Ligase buffersApplications• Blunting of DNA ends fill in of 5 overhangs or and removal of 3 overhangs see​ References1 2 • Blunting of PCR products with 3 dA overhangs• Synthesis of labeled DNA probes by the replacement reaction see​ Reference3 • Oligonucleotide directed site specific mutagenesis see​ Reference4 • Ligation independent cloning of PCR products
    https://www.bioz.com/result/1x t4 dna polymerase buffer/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    1x t4 dna polymerase buffer - by Bioz Stars, 2020-05
    99/100 stars

    Related Products / Commonly Used Together

    second strand synthesis primer

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Anti-cytokine autoantibodies suggest pathogenetic links with autoimmune regulator deficiency in humans and mice
    Article Snippet: .. The PCR products were ligated into the Bam HI/ Not I site of pPK-CMV-F4 (PromoCell GmbH, Heidelberg, Germany) mammalian expression vector using T4 ligase (Invitrogen, Carlsbad, CA, USA). .. All plasmids containing correct inserts (as confirmed by DNA sequencing) were propagated in Escherichia coli NOVA XG cells, amplified, extracted and purified using conventional methods.

    Incubation:

    Article Title: A Rapid Cloning Method Employing Orthogonal End Protection
    Article Snippet: .. Equal molar amounts (typically 250–500 ng at ∼ 100 – 250 ng/µl ) of orthogonally protected synthons were mixed, 0.5–1 unit T4 ligase (Fermentas) and T4 ligase buffer (Fermentas) were added and the ligation mixture was incubated for 10–20 min at 16°C. .. Adding additional ligase had little effect on ligation efficiency.

    Expressing:

    Article Title: Anti-cytokine autoantibodies suggest pathogenetic links with autoimmune regulator deficiency in humans and mice
    Article Snippet: .. The PCR products were ligated into the Bam HI/ Not I site of pPK-CMV-F4 (PromoCell GmbH, Heidelberg, Germany) mammalian expression vector using T4 ligase (Invitrogen, Carlsbad, CA, USA). .. All plasmids containing correct inserts (as confirmed by DNA sequencing) were propagated in Escherichia coli NOVA XG cells, amplified, extracted and purified using conventional methods.

    Plasmid Preparation:

    Article Title: Anti-cytokine autoantibodies suggest pathogenetic links with autoimmune regulator deficiency in humans and mice
    Article Snippet: .. The PCR products were ligated into the Bam HI/ Not I site of pPK-CMV-F4 (PromoCell GmbH, Heidelberg, Germany) mammalian expression vector using T4 ligase (Invitrogen, Carlsbad, CA, USA). .. All plasmids containing correct inserts (as confirmed by DNA sequencing) were propagated in Escherichia coli NOVA XG cells, amplified, extracted and purified using conventional methods.

    Ligation:

    Article Title: Golden GATEway Cloning - A Combinatorial Approach to Generate Fusion and Recombination Constructs
    Article Snippet: .. The 10nM annealed double-stranded oligo dilution was used as an insert in a standard ligation reaction with T4 ligase (5U, Thermo, Fisher) .. Golden Gate Protocol Reactions were set up using 20 fmol of each Golden Gate entry vector and the destination vector, 10 U of BsaI Restriction Enzyme (Thermo, Fisher Fast Digest Enzyme Eco31I) and 30 U of T4 DNA Ligase (Thermo, Fisher) in 2 µl 10x ligation buffer (10x ligation buffer was prepared by supplementing the Thermo, Fisher FastDigest buffer with 10mM dATP and 100mM DTT) to a final reaction volume of 20 µl with ddH2O.

    Article Title: A Rapid Cloning Method Employing Orthogonal End Protection
    Article Snippet: .. Equal molar amounts (typically 250–500 ng at ∼ 100 – 250 ng/µl ) of orthogonally protected synthons were mixed, 0.5–1 unit T4 ligase (Fermentas) and T4 ligase buffer (Fermentas) were added and the ligation mixture was incubated for 10–20 min at 16°C. .. Adding additional ligase had little effect on ligation efficiency.

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  • 99
    Thermo Fisher 1x t4 dna polymerase buffer
    1x T4 Dna Polymerase Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/1x t4 dna polymerase buffer/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    1x t4 dna polymerase buffer - by Bioz Stars, 2020-05
    99/100 stars
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