1x sds gel loading buffer  (Eppendorf AG)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 86
      Buy from Supplier

    Structured Review

    Eppendorf AG 1x sds gel loading buffer
    1x Sds Gel Loading Buffer, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/1x sds gel loading buffer/product/Eppendorf AG
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    1x sds gel loading buffer - by Bioz Stars, 2024-10
    86/100 stars

    Images

    1x sodium dodecyl sulfatepolyacrylamide gel electrophoresis sds page sample loading buffer  (Beyotime)

     
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 86
      Buy from Supplier

    Structured Review

    Beyotime 1x sodium dodecyl sulfatepolyacrylamide gel electrophoresis sds page sample loading buffer
    1x Sodium Dodecyl Sulfatepolyacrylamide Gel Electrophoresis Sds Page Sample Loading Buffer, supplied by Beyotime, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/1x sodium dodecyl sulfatepolyacrylamide gel electrophoresis sds page sample loading buffer/product/Beyotime
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    1x sodium dodecyl sulfatepolyacrylamide gel electrophoresis sds page sample loading buffer - by Bioz Stars, 2024-10
    86/100 stars

    Images

    1x sds page gel loading buffer  (Proteintech)

     
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 86
      Buy from Supplier

    Structured Review

    Proteintech 1x sds page gel loading buffer
    A. Structures of AA132 and AA147 with 2-amino-p-cresol substructure highlighted in red. B. Mechanism of AA132 metabolic activation and covalent protein modification C. <t>Representative</t> <t>SDS-PAGE</t> gel of Cy5-conjugated proteins from HEK293T cells treated for 4 h with vehicle (0.1% DMSO), AA147 yne (10 μM), the combination of AA147 yne (10 μM) and AA147 (40 μM), or the combination of AA147 yne (10 μM) and AA132 (40 μM). D. Bar graph showing the activation of the ERSE.FLuc ATF6 reporter in HEK293T cells treated with AA132 (10 μM) or thapsigargin (Tg, 500 nM) +/− β-mercaptoethanol (BME; 55 μM or 110 μM) for 18 hr. Error bars show SEM for 6 independent experiments . *** p < 0.001, **** p < 0.0001. E. Bar graph showing the activation of the ERSE.FLuc ATF6 reporter in HEK293T cells treated with AA132 (10 μM) or Tg (500 nM) +/− resveratrol (2.5 μM) for 18 hr. Error bars show SEM for 6 independent experiments. **** p < 0.0001.
    1x Sds Page Gel Loading Buffer, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/1x sds page gel loading buffer/product/Proteintech
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    1x sds page gel loading buffer - by Bioz Stars, 2024-10
    86/100 stars

    Images

    1) Product Images from "Divergent Proteome Reactivity Influences Arm-Selective Activation of Pharmacological Endoplasmic Reticulum Proteostasis Regulators"

    Article Title: Divergent Proteome Reactivity Influences Arm-Selective Activation of Pharmacological Endoplasmic Reticulum Proteostasis Regulators

    Journal: bioRxiv

    doi: 10.1101/2023.01.16.524237

    A. Structures of AA132 and AA147 with 2-amino-p-cresol substructure highlighted in red. B. Mechanism of AA132 metabolic activation and covalent protein modification C. Representative SDS-PAGE gel of Cy5-conjugated proteins from HEK293T cells treated for 4 h with vehicle (0.1% DMSO), AA147 yne (10 μM), the combination of AA147 yne (10 μM) and AA147 (40 μM), or the combination of AA147 yne (10 μM) and AA132 (40 μM). D. Bar graph showing the activation of the ERSE.FLuc ATF6 reporter in HEK293T cells treated with AA132 (10 μM) or thapsigargin (Tg, 500 nM) +/− β-mercaptoethanol (BME; 55 μM or 110 μM) for 18 hr. Error bars show SEM for 6 independent experiments . *** p < 0.001, **** p < 0.0001. E. Bar graph showing the activation of the ERSE.FLuc ATF6 reporter in HEK293T cells treated with AA132 (10 μM) or Tg (500 nM) +/− resveratrol (2.5 μM) for 18 hr. Error bars show SEM for 6 independent experiments. **** p < 0.0001.
    Figure Legend Snippet: A. Structures of AA132 and AA147 with 2-amino-p-cresol substructure highlighted in red. B. Mechanism of AA132 metabolic activation and covalent protein modification C. Representative SDS-PAGE gel of Cy5-conjugated proteins from HEK293T cells treated for 4 h with vehicle (0.1% DMSO), AA147 yne (10 μM), the combination of AA147 yne (10 μM) and AA147 (40 μM), or the combination of AA147 yne (10 μM) and AA132 (40 μM). D. Bar graph showing the activation of the ERSE.FLuc ATF6 reporter in HEK293T cells treated with AA132 (10 μM) or thapsigargin (Tg, 500 nM) +/− β-mercaptoethanol (BME; 55 μM or 110 μM) for 18 hr. Error bars show SEM for 6 independent experiments . *** p < 0.001, **** p < 0.0001. E. Bar graph showing the activation of the ERSE.FLuc ATF6 reporter in HEK293T cells treated with AA132 (10 μM) or Tg (500 nM) +/− resveratrol (2.5 μM) for 18 hr. Error bars show SEM for 6 independent experiments. **** p < 0.0001.

    Techniques Used: Activation Assay, Modification, SDS Page

    A. Synthetic scheme for synthesis of AA132 yne . B. Bar graph showing the activation of the ERSE.FLuc ATF6 reporter in HEK293T cells treated with Veh (0.1% DMSO), thapsigargin (Tg; 500 nM), AA132(10 μM), or AA132 yne (10 μM) for 18 hr. ** p < 0.01, *** p < 0.001. C. Graph showing qPCR of the ATF6 target gene BiP, PERK target gene CHOP , and XBP1s target gene DNAJB9 in HEK293T cells treated for 6 h with the indicated compound (10 μM). N = 3 biological replicates. * p < 0.05, ** p < 0.01, *** p < 0.001. D . Representative SDS-PAGE gel of Cy5-conjugated proteins from HEK293T cells treated for 4h with vehicle (0.1% DMSO), AA132 yne (10 μM), the combination of AA132 yne (10 μM) and AA147 (40 μM), or the combination of AA132 yne (10 μM) and AA132 (40 μM).
    Figure Legend Snippet: A. Synthetic scheme for synthesis of AA132 yne . B. Bar graph showing the activation of the ERSE.FLuc ATF6 reporter in HEK293T cells treated with Veh (0.1% DMSO), thapsigargin (Tg; 500 nM), AA132(10 μM), or AA132 yne (10 μM) for 18 hr. ** p < 0.01, *** p < 0.001. C. Graph showing qPCR of the ATF6 target gene BiP, PERK target gene CHOP , and XBP1s target gene DNAJB9 in HEK293T cells treated for 6 h with the indicated compound (10 μM). N = 3 biological replicates. * p < 0.05, ** p < 0.01, *** p < 0.001. D . Representative SDS-PAGE gel of Cy5-conjugated proteins from HEK293T cells treated for 4h with vehicle (0.1% DMSO), AA132 yne (10 μM), the combination of AA132 yne (10 μM) and AA147 (40 μM), or the combination of AA132 yne (10 μM) and AA132 (40 μM).

    Techniques Used: Activation Assay, SDS Page

    A. Representative SDS-PAGE gel of Cy5-conjugated proteins from HEK293T cells treated for the indicated time point with AA147 yne (10 μM). B. Representative SDS-PAGE gel of Cy5-conjugated proteins from HEK293T cells treated for the indicated time point with AA132 yne (10 μM). C. Quantification of gels described in and . Y-axis labeling relative intensity represents PDIA4 intensity normalized to labeling at 6h. Error bars represent S.E.M (N = 4 Biological Replicates). * p < 0.05, ** p < 0.01.
    Figure Legend Snippet: A. Representative SDS-PAGE gel of Cy5-conjugated proteins from HEK293T cells treated for the indicated time point with AA147 yne (10 μM). B. Representative SDS-PAGE gel of Cy5-conjugated proteins from HEK293T cells treated for the indicated time point with AA132 yne (10 μM). C. Quantification of gels described in and . Y-axis labeling relative intensity represents PDIA4 intensity normalized to labeling at 6h. Error bars represent S.E.M (N = 4 Biological Replicates). * p < 0.05, ** p < 0.01.

    Techniques Used: SDS Page, Labeling

    1x sds page gel loading buffer  (Proteintech)

     
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 86
      Buy from Supplier

    Structured Review

    Proteintech 1x sds page gel loading buffer
    1x Sds Page Gel Loading Buffer, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/1x sds page gel loading buffer/product/Proteintech
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    1x sds page gel loading buffer - by Bioz Stars, 2024-10
    86/100 stars

    Images

    1x sds page gel loading buffer  (Proteintech)

     
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 86
      Buy from Supplier

    Structured Review

    Proteintech 1x sds page gel loading buffer
    A. Structures of AA132 and AA147 with 2-amino- p -cresol substructure highlighted in red. B. Mechanism of AA132 metabolic activation and covalent protein modification C. <t>Representative</t> <t>SDS-PAGE</t> gel of Cy5-conjugated proteins from HEK293T cells treated for 4 h with vehicle (0.1% DMSO), AA147 yne (10 µM), the combination of AA147 yne (10 µM) and AA147 (40 µM), or the combination of AA147 yne (10 µM) and AA132 (40 µM). D. Bar graph showing the activation of the ERSE.FLuc ATF6 reporter in HEK293T cells treated with AA132 (10 µM) or thapsigargin (Tg, 500 nM) +/- β-mercaptoethanol (BME; 55 μM or 110 μM) for 18 hr. Error bars show SEM for 6 independent experiments. *** p < 0.001, **** p < 0.0001. E. Bar graph showing the activation of the ERSE.FLuc ATF6 reporter in HEK293T cells treated with AA132 (10 µM) or Tg (500 nM) +/- resveratrol (2.5 µM) for 18 hr. Error bars show SEM for 6 independent experiments. **** p < 0.0001.
    1x Sds Page Gel Loading Buffer, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/1x sds page gel loading buffer/product/Proteintech
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    1x sds page gel loading buffer - by Bioz Stars, 2024-10
    86/100 stars

    Images

    1) Product Images from "Divergent Proteome Reactivity Influences Arm-Selective Activation of Pharmacological Endoplasmic Reticulum Proteostasis Regulators"

    Article Title: Divergent Proteome Reactivity Influences Arm-Selective Activation of Pharmacological Endoplasmic Reticulum Proteostasis Regulators

    Journal: bioRxiv

    doi: 10.1101/2023.01.16.524237

    A. Structures of AA132 and AA147 with 2-amino- p -cresol substructure highlighted in red. B. Mechanism of AA132 metabolic activation and covalent protein modification C. Representative SDS-PAGE gel of Cy5-conjugated proteins from HEK293T cells treated for 4 h with vehicle (0.1% DMSO), AA147 yne (10 µM), the combination of AA147 yne (10 µM) and AA147 (40 µM), or the combination of AA147 yne (10 µM) and AA132 (40 µM). D. Bar graph showing the activation of the ERSE.FLuc ATF6 reporter in HEK293T cells treated with AA132 (10 µM) or thapsigargin (Tg, 500 nM) +/- β-mercaptoethanol (BME; 55 μM or 110 μM) for 18 hr. Error bars show SEM for 6 independent experiments. *** p < 0.001, **** p < 0.0001. E. Bar graph showing the activation of the ERSE.FLuc ATF6 reporter in HEK293T cells treated with AA132 (10 µM) or Tg (500 nM) +/- resveratrol (2.5 µM) for 18 hr. Error bars show SEM for 6 independent experiments. **** p < 0.0001.
    Figure Legend Snippet: A. Structures of AA132 and AA147 with 2-amino- p -cresol substructure highlighted in red. B. Mechanism of AA132 metabolic activation and covalent protein modification C. Representative SDS-PAGE gel of Cy5-conjugated proteins from HEK293T cells treated for 4 h with vehicle (0.1% DMSO), AA147 yne (10 µM), the combination of AA147 yne (10 µM) and AA147 (40 µM), or the combination of AA147 yne (10 µM) and AA132 (40 µM). D. Bar graph showing the activation of the ERSE.FLuc ATF6 reporter in HEK293T cells treated with AA132 (10 µM) or thapsigargin (Tg, 500 nM) +/- β-mercaptoethanol (BME; 55 μM or 110 μM) for 18 hr. Error bars show SEM for 6 independent experiments. *** p < 0.001, **** p < 0.0001. E. Bar graph showing the activation of the ERSE.FLuc ATF6 reporter in HEK293T cells treated with AA132 (10 µM) or Tg (500 nM) +/- resveratrol (2.5 µM) for 18 hr. Error bars show SEM for 6 independent experiments. **** p < 0.0001.

    Techniques Used: Activation Assay, Modification, SDS Page

    A. Synthetic scheme for synthesis of AA132 yne . B. Bar graph showing the activation of the ERSE.FLuc ATF6 reporter in HEK293T cells treated with Veh (0.1% DMSO), thapsigargin (Tg; 500 nM), AA132(10 µM), or AA132 yne (10 µM) for 18 hr. ** p < 0.01, *** p < 0.001. C. Graph showing qPCR of the ATF6 target gene BiP , PERK target gene CHOP , and XBP1s target gene DNAJB9 in HEK293T cells treated for 6 h with the indicated compound (10 µM). N = 3 biological replicates. * p < 0.05, ** p < 0.01, *** p < 0.001. D . Representative SDS-PAGE gel of Cy5-conjugated proteins from HEK293T cells treated for 4h with vehicle (0.1% DMSO), AA132 yne (10 µM), the combination of AA132 yne (10 µM) and AA147 (40 µM), or the combination of AA132 yne (10 µM) and AA132 (40 µM).
    Figure Legend Snippet: A. Synthetic scheme for synthesis of AA132 yne . B. Bar graph showing the activation of the ERSE.FLuc ATF6 reporter in HEK293T cells treated with Veh (0.1% DMSO), thapsigargin (Tg; 500 nM), AA132(10 µM), or AA132 yne (10 µM) for 18 hr. ** p < 0.01, *** p < 0.001. C. Graph showing qPCR of the ATF6 target gene BiP , PERK target gene CHOP , and XBP1s target gene DNAJB9 in HEK293T cells treated for 6 h with the indicated compound (10 µM). N = 3 biological replicates. * p < 0.05, ** p < 0.01, *** p < 0.001. D . Representative SDS-PAGE gel of Cy5-conjugated proteins from HEK293T cells treated for 4h with vehicle (0.1% DMSO), AA132 yne (10 µM), the combination of AA132 yne (10 µM) and AA147 (40 µM), or the combination of AA132 yne (10 µM) and AA132 (40 µM).

    Techniques Used: Activation Assay, SDS Page

    A. Representative immunoblot of the soluble fraction of PDIA1 from heat-treated ALMC2 cells at the temperatures indicated (40-72°C) preincubated with listed compound (10 µM, 2h). B. Graph of percent soluble fraction (y-axis) versus temperature (x-axis) for data in . Fitted curves calculated using Boltzmann Sigmoidal Fit in Prism and plotted with 95% confidence intervals (dotted lines). Tm is temperature on calculated sigmoidal curve with 50% soluble PDIA1 fraction remaining. Error bars represent S.E.M (N = 3 biological replicates). C. Representative SDS-PAGE gel of Cy5-conjugated proteins from ALMC2 cells treated at indicated time point with AA147 yne (10 µM). D. Representative SDS-PAGE gel of Cy5-conjugated proteins from ALMC2 cells treated at indicated time point with AA132 yne (10 µM). E. Representative gel of AA132 yne and AA147 yne labeled proteins in HEK293T cells cotreated with indicated concentrations of β-mercaptoethanol or resveratrol for 4h. F. Quantification of . Error bars represent standard error of mean. p values calculated using two-sided Student T Test. Percent labeling calculated as lane intensity relative to cotreatment with vehicle (0.1% DPBS) * p < 0.05, **p < 0.01.
    Figure Legend Snippet: A. Representative immunoblot of the soluble fraction of PDIA1 from heat-treated ALMC2 cells at the temperatures indicated (40-72°C) preincubated with listed compound (10 µM, 2h). B. Graph of percent soluble fraction (y-axis) versus temperature (x-axis) for data in . Fitted curves calculated using Boltzmann Sigmoidal Fit in Prism and plotted with 95% confidence intervals (dotted lines). Tm is temperature on calculated sigmoidal curve with 50% soluble PDIA1 fraction remaining. Error bars represent S.E.M (N = 3 biological replicates). C. Representative SDS-PAGE gel of Cy5-conjugated proteins from ALMC2 cells treated at indicated time point with AA147 yne (10 µM). D. Representative SDS-PAGE gel of Cy5-conjugated proteins from ALMC2 cells treated at indicated time point with AA132 yne (10 µM). E. Representative gel of AA132 yne and AA147 yne labeled proteins in HEK293T cells cotreated with indicated concentrations of β-mercaptoethanol or resveratrol for 4h. F. Quantification of . Error bars represent standard error of mean. p values calculated using two-sided Student T Test. Percent labeling calculated as lane intensity relative to cotreatment with vehicle (0.1% DPBS) * p < 0.05, **p < 0.01.

    Techniques Used: Western Blot, SDS Page, Labeling

    A. Representative SDS-PAGE gel of Cy5-conjugated proteins from HEK293T cells treated for the indicated time point with AA147 yne (10 µM). B. Representative SDS-PAGE gel of Cy5-conjugated proteins from HEK293T cells treated for the indicated time point with AA132 yne (10 µM). C. Quantification of gels described in and . Y-axis labeling relative intensity represents PDIA4 intensity normalized to labeling at 6h. Error bars represent S.E.M (N = 4 Biological Replicates). * p < 0.05, ** p < 0.01.
    Figure Legend Snippet: A. Representative SDS-PAGE gel of Cy5-conjugated proteins from HEK293T cells treated for the indicated time point with AA147 yne (10 µM). B. Representative SDS-PAGE gel of Cy5-conjugated proteins from HEK293T cells treated for the indicated time point with AA132 yne (10 µM). C. Quantification of gels described in and . Y-axis labeling relative intensity represents PDIA4 intensity normalized to labeling at 6h. Error bars represent S.E.M (N = 4 Biological Replicates). * p < 0.05, ** p < 0.01.

    Techniques Used: SDS Page, Labeling

    A. Fluorescence and coomassie-stained SDS-PAGE of lysates prepared from HEK293T cells treated with the indicated concentration of AA132 yne (4 h) and then conjugated to azide-cyanine. B-D . Top-10 GO terms for significantly induced genes (fold change >1.3, p<0.05) identified by RNAseq in HEK293T cells treated with 10 µM ( A ), 15 µM ( B ), or 30 µM ( C ) AA132 for 6 h. RNAseq data is included in Table S3 Full GO analysis is included in Table S4 .
    Figure Legend Snippet: A. Fluorescence and coomassie-stained SDS-PAGE of lysates prepared from HEK293T cells treated with the indicated concentration of AA132 yne (4 h) and then conjugated to azide-cyanine. B-D . Top-10 GO terms for significantly induced genes (fold change >1.3, p<0.05) identified by RNAseq in HEK293T cells treated with 10 µM ( A ), 15 µM ( B ), or 30 µM ( C ) AA132 for 6 h. RNAseq data is included in Table S3 Full GO analysis is included in Table S4 .

    Techniques Used: Fluorescence, Staining, SDS Page, Concentration Assay

    sds gel loading buffer 1x  (Sigma-Genosys)

     
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 86
      Buy from Supplier

    Structured Review

    Sigma-Genosys sds gel loading buffer 1x
    Sds Gel Loading Buffer 1x, supplied by Sigma-Genosys, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sds gel loading buffer 1x/product/Sigma-Genosys
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sds gel loading buffer 1x - by Bioz Stars, 2024-10
    86/100 stars

    Images

    1x sds gel loading buffer  (Millipore)

     
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 86
      Buy from Supplier

    Structured Review

    Millipore 1x sds gel loading buffer
    1x Sds Gel Loading Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/1x sds gel loading buffer/product/Millipore
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    1x sds gel loading buffer - by Bioz Stars, 2024-10
    86/100 stars

    Images

    1x sds gel loading buffer  (Thermo Fisher)


    Bioz Verified Symbol Thermo Fisher is a verified supplier
    Bioz Manufacturer Symbol Thermo Fisher manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 86
      Buy from Supplier

    Structured Review

    Thermo Fisher 1x sds gel loading buffer
    1x Sds Gel Loading Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/1x sds gel loading buffer/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    1x sds gel loading buffer - by Bioz Stars, 2024-10
    86/100 stars

    Images

    1x sds gel loading buffer  (Millipore)

     
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 86
      Buy from Supplier

    Structured Review

    Millipore 1x sds gel loading buffer
    1x Sds Gel Loading Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/1x sds gel loading buffer/product/Millipore
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    1x sds gel loading buffer - by Bioz Stars, 2024-10
    86/100 stars

    Images

    1x sds gel loading buffer  (Thermo Fisher)


    Bioz Verified Symbol Thermo Fisher is a verified supplier
    Bioz Manufacturer Symbol Thermo Fisher manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 86
      Buy from Supplier

    Structured Review

    Thermo Fisher 1x sds gel loading buffer
    1x Sds Gel Loading Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/1x sds gel loading buffer/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    1x sds gel loading buffer - by Bioz Stars, 2024-10
    86/100 stars

    Images

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 1x sds gel loading buffer  (Eppendorf AG)
    86
    Eppendorf AG 1x sds gel loading buffer
    1x Sds Gel Loading Buffer, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/1x sds gel loading buffer/product/Eppendorf AG
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    1x sds gel loading buffer - by Bioz Stars, 2024-10
    86/100 stars
    		  Buy from Supplier
    1x sodium dodecyl sulfatepolyacrylamide gel electrophoresis sds page sample loading buffer  (Beyotime)
    86
    Beyotime 1x sodium dodecyl sulfatepolyacrylamide gel electrophoresis sds page sample loading buffer
    1x Sodium Dodecyl Sulfatepolyacrylamide Gel Electrophoresis Sds Page Sample Loading Buffer, supplied by Beyotime, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/1x sodium dodecyl sulfatepolyacrylamide gel electrophoresis sds page sample loading buffer/product/Beyotime
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    1x sodium dodecyl sulfatepolyacrylamide gel electrophoresis sds page sample loading buffer - by Bioz Stars, 2024-10
    86/100 stars
    		  Buy from Supplier
    1x sds page gel loading buffer  (Proteintech)
    86
    Proteintech 1x sds page gel loading buffer
    A. Structures of AA132 and AA147 with 2-amino-p-cresol substructure highlighted in red. B. Mechanism of AA132 metabolic activation and covalent protein modification C. <t>Representative</t> <t>SDS-PAGE</t> gel of Cy5-conjugated proteins from HEK293T cells treated for 4 h with vehicle (0.1% DMSO), AA147 yne (10 μM), the combination of AA147 yne (10 μM) and AA147 (40 μM), or the combination of AA147 yne (10 μM) and AA132 (40 μM). D. Bar graph showing the activation of the ERSE.FLuc ATF6 reporter in HEK293T cells treated with AA132 (10 μM) or thapsigargin (Tg, 500 nM) +/− β-mercaptoethanol (BME; 55 μM or 110 μM) for 18 hr. Error bars show SEM for 6 independent experiments . *** p < 0.001, **** p < 0.0001. E. Bar graph showing the activation of the ERSE.FLuc ATF6 reporter in HEK293T cells treated with AA132 (10 μM) or Tg (500 nM) +/− resveratrol (2.5 μM) for 18 hr. Error bars show SEM for 6 independent experiments. **** p < 0.0001.
    1x Sds Page Gel Loading Buffer, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/1x sds page gel loading buffer/product/Proteintech
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    1x sds page gel loading buffer - by Bioz Stars, 2024-10
    86/100 stars
    		  Buy from Supplier
    sds gel loading buffer 1x  (Sigma-Genosys)
    86
    Sigma-Genosys sds gel loading buffer 1x
    A. Structures of AA132 and AA147 with 2-amino-p-cresol substructure highlighted in red. B. Mechanism of AA132 metabolic activation and covalent protein modification C. <t>Representative</t> <t>SDS-PAGE</t> gel of Cy5-conjugated proteins from HEK293T cells treated for 4 h with vehicle (0.1% DMSO), AA147 yne (10 μM), the combination of AA147 yne (10 μM) and AA147 (40 μM), or the combination of AA147 yne (10 μM) and AA132 (40 μM). D. Bar graph showing the activation of the ERSE.FLuc ATF6 reporter in HEK293T cells treated with AA132 (10 μM) or thapsigargin (Tg, 500 nM) +/− β-mercaptoethanol (BME; 55 μM or 110 μM) for 18 hr. Error bars show SEM for 6 independent experiments . *** p < 0.001, **** p < 0.0001. E. Bar graph showing the activation of the ERSE.FLuc ATF6 reporter in HEK293T cells treated with AA132 (10 μM) or Tg (500 nM) +/− resveratrol (2.5 μM) for 18 hr. Error bars show SEM for 6 independent experiments. **** p < 0.0001.
    Sds Gel Loading Buffer 1x, supplied by Sigma-Genosys, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sds gel loading buffer 1x/product/Sigma-Genosys
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sds gel loading buffer 1x - by Bioz Stars, 2024-10
    86/100 stars
    		  Buy from Supplier
    1x sds gel loading buffer  (Millipore)
    86
    Millipore 1x sds gel loading buffer
    A. Structures of AA132 and AA147 with 2-amino-p-cresol substructure highlighted in red. B. Mechanism of AA132 metabolic activation and covalent protein modification C. <t>Representative</t> <t>SDS-PAGE</t> gel of Cy5-conjugated proteins from HEK293T cells treated for 4 h with vehicle (0.1% DMSO), AA147 yne (10 μM), the combination of AA147 yne (10 μM) and AA147 (40 μM), or the combination of AA147 yne (10 μM) and AA132 (40 μM). D. Bar graph showing the activation of the ERSE.FLuc ATF6 reporter in HEK293T cells treated with AA132 (10 μM) or thapsigargin (Tg, 500 nM) +/− β-mercaptoethanol (BME; 55 μM or 110 μM) for 18 hr. Error bars show SEM for 6 independent experiments . *** p < 0.001, **** p < 0.0001. E. Bar graph showing the activation of the ERSE.FLuc ATF6 reporter in HEK293T cells treated with AA132 (10 μM) or Tg (500 nM) +/− resveratrol (2.5 μM) for 18 hr. Error bars show SEM for 6 independent experiments. **** p < 0.0001.
    1x Sds Gel Loading Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/1x sds gel loading buffer/product/Millipore
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    1x sds gel loading buffer - by Bioz Stars, 2024-10
    86/100 stars
    		  Buy from Supplier
    1x sds gel loading buffer  (Thermo Fisher)
    86
    Thermo Fisher 1x sds gel loading buffer
    A. Structures of AA132 and AA147 with 2-amino-p-cresol substructure highlighted in red. B. Mechanism of AA132 metabolic activation and covalent protein modification C. <t>Representative</t> <t>SDS-PAGE</t> gel of Cy5-conjugated proteins from HEK293T cells treated for 4 h with vehicle (0.1% DMSO), AA147 yne (10 μM), the combination of AA147 yne (10 μM) and AA147 (40 μM), or the combination of AA147 yne (10 μM) and AA132 (40 μM). D. Bar graph showing the activation of the ERSE.FLuc ATF6 reporter in HEK293T cells treated with AA132 (10 μM) or thapsigargin (Tg, 500 nM) +/− β-mercaptoethanol (BME; 55 μM or 110 μM) for 18 hr. Error bars show SEM for 6 independent experiments . *** p < 0.001, **** p < 0.0001. E. Bar graph showing the activation of the ERSE.FLuc ATF6 reporter in HEK293T cells treated with AA132 (10 μM) or Tg (500 nM) +/− resveratrol (2.5 μM) for 18 hr. Error bars show SEM for 6 independent experiments. **** p < 0.0001.
    1x Sds Gel Loading Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/1x sds gel loading buffer/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    1x sds gel loading buffer - by Bioz Stars, 2024-10
    86/100 stars
    		  Buy from Supplier

    Image Search Results


    A. Structures of AA132 and AA147 with 2-amino-p-cresol substructure highlighted in red. B. Mechanism of AA132 metabolic activation and covalent protein modification C. Representative SDS-PAGE gel of Cy5-conjugated proteins from HEK293T cells treated for 4 h with vehicle (0.1% DMSO), AA147 yne (10 μM), the combination of AA147 yne (10 μM) and AA147 (40 μM), or the combination of AA147 yne (10 μM) and AA132 (40 μM). D. Bar graph showing the activation of the ERSE.FLuc ATF6 reporter in HEK293T cells treated with AA132 (10 μM) or thapsigargin (Tg, 500 nM) +/− β-mercaptoethanol (BME; 55 μM or 110 μM) for 18 hr. Error bars show SEM for 6 independent experiments . *** p < 0.001, **** p < 0.0001. E. Bar graph showing the activation of the ERSE.FLuc ATF6 reporter in HEK293T cells treated with AA132 (10 μM) or Tg (500 nM) +/− resveratrol (2.5 μM) for 18 hr. Error bars show SEM for 6 independent experiments. **** p < 0.0001.

    Journal: bioRxiv

    Article Title: Divergent Proteome Reactivity Influences Arm-Selective Activation of Pharmacological Endoplasmic Reticulum Proteostasis Regulators

    doi: 10.1101/2023.01.16.524237

    Figure Lengend Snippet: A. Structures of AA132 and AA147 with 2-amino-p-cresol substructure highlighted in red. B. Mechanism of AA132 metabolic activation and covalent protein modification C. Representative SDS-PAGE gel of Cy5-conjugated proteins from HEK293T cells treated for 4 h with vehicle (0.1% DMSO), AA147 yne (10 μM), the combination of AA147 yne (10 μM) and AA147 (40 μM), or the combination of AA147 yne (10 μM) and AA132 (40 μM). D. Bar graph showing the activation of the ERSE.FLuc ATF6 reporter in HEK293T cells treated with AA132 (10 μM) or thapsigargin (Tg, 500 nM) +/− β-mercaptoethanol (BME; 55 μM or 110 μM) for 18 hr. Error bars show SEM for 6 independent experiments . *** p < 0.001, **** p < 0.0001. E. Bar graph showing the activation of the ERSE.FLuc ATF6 reporter in HEK293T cells treated with AA132 (10 μM) or Tg (500 nM) +/− resveratrol (2.5 μM) for 18 hr. Error bars show SEM for 6 independent experiments. **** p < 0.0001.

    Article Snippet: Proteins resuspended in 1x SDS-PAGE gel loading buffer (20 μL) and heated for 5 min at 95 °C prior to separation via SDS-PAGE and transfer to PVDF membranes for immunoblot analysis of indicated proteins (rabbit PDIA4 (1:2000) (Protein Tech), rabbit PDIA3 (1:1000) (Protein Tech), mouse GAPDH (1:1000) (Cell Signaling), Goat anti-rabbit IRdye 800-cw (Licor) (1:10000), Goat anti-mouse IRdye-680RD (Licor) (1:10000).

    Techniques: Activation Assay, Modification, SDS Page

    A. Synthetic scheme for synthesis of AA132 yne . B. Bar graph showing the activation of the ERSE.FLuc ATF6 reporter in HEK293T cells treated with Veh (0.1% DMSO), thapsigargin (Tg; 500 nM), AA132(10 μM), or AA132 yne (10 μM) for 18 hr. ** p < 0.01, *** p < 0.001. C. Graph showing qPCR of the ATF6 target gene BiP, PERK target gene CHOP , and XBP1s target gene DNAJB9 in HEK293T cells treated for 6 h with the indicated compound (10 μM). N = 3 biological replicates. * p < 0.05, ** p < 0.01, *** p < 0.001. D . Representative SDS-PAGE gel of Cy5-conjugated proteins from HEK293T cells treated for 4h with vehicle (0.1% DMSO), AA132 yne (10 μM), the combination of AA132 yne (10 μM) and AA147 (40 μM), or the combination of AA132 yne (10 μM) and AA132 (40 μM).

    Journal: bioRxiv

    Article Title: Divergent Proteome Reactivity Influences Arm-Selective Activation of Pharmacological Endoplasmic Reticulum Proteostasis Regulators

    doi: 10.1101/2023.01.16.524237

    Figure Lengend Snippet: A. Synthetic scheme for synthesis of AA132 yne . B. Bar graph showing the activation of the ERSE.FLuc ATF6 reporter in HEK293T cells treated with Veh (0.1% DMSO), thapsigargin (Tg; 500 nM), AA132(10 μM), or AA132 yne (10 μM) for 18 hr. ** p < 0.01, *** p < 0.001. C. Graph showing qPCR of the ATF6 target gene BiP, PERK target gene CHOP , and XBP1s target gene DNAJB9 in HEK293T cells treated for 6 h with the indicated compound (10 μM). N = 3 biological replicates. * p < 0.05, ** p < 0.01, *** p < 0.001. D . Representative SDS-PAGE gel of Cy5-conjugated proteins from HEK293T cells treated for 4h with vehicle (0.1% DMSO), AA132 yne (10 μM), the combination of AA132 yne (10 μM) and AA147 (40 μM), or the combination of AA132 yne (10 μM) and AA132 (40 μM).

    Article Snippet: Proteins resuspended in 1x SDS-PAGE gel loading buffer (20 μL) and heated for 5 min at 95 °C prior to separation via SDS-PAGE and transfer to PVDF membranes for immunoblot analysis of indicated proteins (rabbit PDIA4 (1:2000) (Protein Tech), rabbit PDIA3 (1:1000) (Protein Tech), mouse GAPDH (1:1000) (Cell Signaling), Goat anti-rabbit IRdye 800-cw (Licor) (1:10000), Goat anti-mouse IRdye-680RD (Licor) (1:10000).

    Techniques: Activation Assay, SDS Page

    A. Representative SDS-PAGE gel of Cy5-conjugated proteins from HEK293T cells treated for the indicated time point with AA147 yne (10 μM). B. Representative SDS-PAGE gel of Cy5-conjugated proteins from HEK293T cells treated for the indicated time point with AA132 yne (10 μM). C. Quantification of gels described in and . Y-axis labeling relative intensity represents PDIA4 intensity normalized to labeling at 6h. Error bars represent S.E.M (N = 4 Biological Replicates). * p < 0.05, ** p < 0.01.

    Journal: bioRxiv

    Article Title: Divergent Proteome Reactivity Influences Arm-Selective Activation of Pharmacological Endoplasmic Reticulum Proteostasis Regulators

    doi: 10.1101/2023.01.16.524237

    Figure Lengend Snippet: A. Representative SDS-PAGE gel of Cy5-conjugated proteins from HEK293T cells treated for the indicated time point with AA147 yne (10 μM). B. Representative SDS-PAGE gel of Cy5-conjugated proteins from HEK293T cells treated for the indicated time point with AA132 yne (10 μM). C. Quantification of gels described in and . Y-axis labeling relative intensity represents PDIA4 intensity normalized to labeling at 6h. Error bars represent S.E.M (N = 4 Biological Replicates). * p < 0.05, ** p < 0.01.

    Article Snippet: Proteins resuspended in 1x SDS-PAGE gel loading buffer (20 μL) and heated for 5 min at 95 °C prior to separation via SDS-PAGE and transfer to PVDF membranes for immunoblot analysis of indicated proteins (rabbit PDIA4 (1:2000) (Protein Tech), rabbit PDIA3 (1:1000) (Protein Tech), mouse GAPDH (1:1000) (Cell Signaling), Goat anti-rabbit IRdye 800-cw (Licor) (1:10000), Goat anti-mouse IRdye-680RD (Licor) (1:10000).

    Techniques: SDS Page, Labeling