1x roche mini complete protease inhibitor cocktail solution  (Roche)

 
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    Structured Review

    Roche 1x roche mini complete protease inhibitor cocktail solution
    1x Roche Mini Complete Protease Inhibitor Cocktail Solution, supplied by Roche, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/1x roche mini complete protease inhibitor cocktail solution/product/Roche
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    1x roche mini complete protease inhibitor cocktail solution - by Bioz Stars, 2020-04
    91/100 stars

    Related Products / Commonly Used Together

    electrophoretic mobility shift assay emsa nuclear protein

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    Negative Control:

    Article Title: siRNA-induced TRAF6 knockdown promotes the apoptosis and inhibits the invasion of human lung cancer SPC-A1 cells
    Article Snippet: Electrophoretic mobility shift assay (EMSA) Nuclear protein was extracted as described previously, and all nuclear extractions contained 1X Roche Mini Complete protease inhibitor cocktail solution (Roche Applied Science, Burgess Hill, UK). .. An amount of 10 mg of nuclear extract was used per binding reaction as detailed in the manufacturer's protocol, with some control reactions using NF-κB unlabeled oligonucleotides or nonspecific negative control OCT1 consensus oligonucleotides (Promega) for competition or the NF-κB p65 antibody (Thermo Fisher Scientific) for supershift reactions.

    Protease Inhibitor:

    Article Title: siRNA-induced TRAF6 knockdown promotes the apoptosis and inhibits the invasion of human lung cancer SPC-A1 cells
    Article Snippet: .. Electrophoretic mobility shift assay (EMSA) Nuclear protein was extracted as described previously, and all nuclear extractions contained 1X Roche Mini Complete protease inhibitor cocktail solution (Roche Applied Science, Burgess Hill, UK). .. A 22-mer NF-κB consensus oligonucleotide (Promega) was labeled according to the manufacturer's instructions using the DIG gel-shift kit (Roche Applied Science).

    Immunodetection:

    Article Title: siRNA-induced TRAF6 knockdown promotes the apoptosis and inhibits the invasion of human lung cancer SPC-A1 cells
    Article Snippet: Electrophoretic mobility shift assay (EMSA) Nuclear protein was extracted as described previously, and all nuclear extractions contained 1X Roche Mini Complete protease inhibitor cocktail solution (Roche Applied Science, Burgess Hill, UK). .. Reactions were run on 6% (v/v) DNA retardation gels (Invitrogen, Paisley, UK), and then blotted and cross-linked onto positively charged nylon membranes (Roche Applied Science) before immunodetection.

    Electrophoretic Mobility Shift Assay:

    Article Title: siRNA-induced TRAF6 knockdown promotes the apoptosis and inhibits the invasion of human lung cancer SPC-A1 cells
    Article Snippet: .. Electrophoretic mobility shift assay (EMSA) Nuclear protein was extracted as described previously, and all nuclear extractions contained 1X Roche Mini Complete protease inhibitor cocktail solution (Roche Applied Science, Burgess Hill, UK). .. A 22-mer NF-κB consensus oligonucleotide (Promega) was labeled according to the manufacturer's instructions using the DIG gel-shift kit (Roche Applied Science).

    Labeling:

    Article Title: siRNA-induced TRAF6 knockdown promotes the apoptosis and inhibits the invasion of human lung cancer SPC-A1 cells
    Article Snippet: Electrophoretic mobility shift assay (EMSA) Nuclear protein was extracted as described previously, and all nuclear extractions contained 1X Roche Mini Complete protease inhibitor cocktail solution (Roche Applied Science, Burgess Hill, UK). .. A 22-mer NF-κB consensus oligonucleotide (Promega) was labeled according to the manufacturer's instructions using the DIG gel-shift kit (Roche Applied Science).

    Binding Assay:

    Article Title: siRNA-induced TRAF6 knockdown promotes the apoptosis and inhibits the invasion of human lung cancer SPC-A1 cells
    Article Snippet: Electrophoretic mobility shift assay (EMSA) Nuclear protein was extracted as described previously, and all nuclear extractions contained 1X Roche Mini Complete protease inhibitor cocktail solution (Roche Applied Science, Burgess Hill, UK). .. An amount of 10 mg of nuclear extract was used per binding reaction as detailed in the manufacturer's protocol, with some control reactions using NF-κB unlabeled oligonucleotides or nonspecific negative control OCT1 consensus oligonucleotides (Promega) for competition or the NF-κB p65 antibody (Thermo Fisher Scientific) for supershift reactions.

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  • 91
    Roche 1x roche mini complete protease inhibitor cocktail solution
    1x Roche Mini Complete Protease Inhibitor Cocktail Solution, supplied by Roche, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/1x roche mini complete protease inhibitor cocktail solution/product/Roche
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    1x roche mini complete protease inhibitor cocktail solution - by Bioz Stars, 2020-04
    91/100 stars
      Buy from Supplier

    87
    Roche mini complete protease inhibitor cocktail solution
    Mini Complete Protease Inhibitor Cocktail Solution, supplied by Roche, used in various techniques. Bioz Stars score: 87/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mini complete protease inhibitor cocktail solution/product/Roche
    Average 87 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mini complete protease inhibitor cocktail solution - by Bioz Stars, 2020-04
    87/100 stars
      Buy from Supplier

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