Structured Review

Thermo Fisher 1x pbs t
1x Pbs T, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/1x pbs t/product/Thermo Fisher
Average 89 stars, based on 1 article reviews
Price from $9.99 to $1999.99
1x pbs t - by Bioz Stars, 2020-07
89/100 stars

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Staining:

Article Title: Crosstalk between mitogen-activated protein kinase inhibitors and transforming growth factor-β signaling results in variable activation of human dermal fibroblasts
Article Snippet: .. Cells were then washed three times for 5 min each in PBS-T prior to being incubated in 500 ng/ml Hoechst 33342 in 1X PBS-T (Thermo Fisher Scientific, Inc.) for 15 min at room temperature to stain the nuclei. .. Cells were washed twice for 5 min each in PBS and coverslips were mounted onto glass slides using Prolong Gold (Thermo Fisher Scientific, Inc.) and stored at 4°C until imaging.

Incubation:

Article Title: Crosstalk between mitogen-activated protein kinase inhibitors and transforming growth factor-β signaling results in variable activation of human dermal fibroblasts
Article Snippet: .. Cells were then washed three times for 5 min each in PBS-T prior to being incubated in 500 ng/ml Hoechst 33342 in 1X PBS-T (Thermo Fisher Scientific, Inc.) for 15 min at room temperature to stain the nuclei. .. Cells were washed twice for 5 min each in PBS and coverslips were mounted onto glass slides using Prolong Gold (Thermo Fisher Scientific, Inc.) and stored at 4°C until imaging.

Article Title: A Comparison of Neuroinflammation to Implanted Microelectrodes in Rat and Mouse Models
Article Snippet: .. Unbound primary antibodies were then removed using six subsequent washes with 1X PBS-T. Next, species-specific secondary antibodies (anti-rabbit, anti-goat, anti-mouse or anti-rat Alexa-Fluor 488 and anti-rabbit, anti-goat or anti-mouse Alexa-Fluor 594, Molecular Probes) were diluted 1:1000 in blocking buffer and incubated with tissue sections for two hours at RT. .. Total cell population was also labeled in all tissue samples using 4′,6-diamidino-2-phenylindole (DAPI, Life Technologies).

Article Title: Implications of Chronic Daily Anti-Oxidant Administration on the Inflammatory Response to Intracortical Microelectrodes
Article Snippet: .. Following blocking, the primary antibody, diluted in blocking buffer, was added directly to tissue sections and allowed to incubate for 18 hours at 4° C. Unbound primary antibodies were removed using six subsequent washes in 1X PBS-T. Species-specific secondary antibodies (anti-rabbit or mouse Alexa-Fluor 488 and anti-rabbit or mouse Alexa-Fluor 594, Life Technologies) were diluted 1:1000 in blocking buffer, applied directly to tissue sections and incubated at room temperature for 2 hours. .. In addition to secondary antibodies, 4’6-diamidino-2-phenylindole (DAPI; Life Technologies) was diluted at 1:32,000 to co-label total cell nuclei.

Article Title: Condensin-Driven Remodeling of X-Chromosome Topology during Dosage Compensation
Article Snippet: .. Slides were washed 3 times 10 min with 1X PBS-T at room temperature and then incubated in secondary antibody (Alexa-Fluor-647 goat anti-rat antibody (Invitrogen), 1:250) for 6 hrs to overnight. ..

Imaging:

Article Title: Enhanced gut barrier integrity sensitizes colon cancer to immune therapy
Article Snippet: .. Sections were washed twice with 1X PBS-T and Prolong Gold anti-fade reagent (Thermo Fisher, Waltham, MA) was added to the slides prior to imaging. .. Images were captured using a Leica SP5 confocal laser scanning microscope (Leica, Wetzlar, Germany) and processed using Fiji Software.

Blocking Assay:

Article Title: Transverse tubule remodeling enhances Orai1-dependent Ca2+ entry in skeletal muscle
Article Snippet: .. Following blocking for 1 hr at room temperature in 10% BSA in 1X PBS-T (PBS + 0.1% Triton X-100), fibers were co-labeled overnight at 4°C using primary antibodies for GFP (Rabbit polyclonal antibody, Thermo Fisher Scientific, Cat. # A11122, 1:3000) and either α-actinin (Mouse anti α-actinin, Sigma, Cat # A7811, 1:750) or the type1 ryanodine receptor (RYR1- Mouse anti RYR1 Cat # 34C, DHSB University of Iowa, 1:30). ..

Article Title: A Comparison of Neuroinflammation to Implanted Microelectrodes in Rat and Mouse Models
Article Snippet: .. Unbound primary antibodies were then removed using six subsequent washes with 1X PBS-T. Next, species-specific secondary antibodies (anti-rabbit, anti-goat, anti-mouse or anti-rat Alexa-Fluor 488 and anti-rabbit, anti-goat or anti-mouse Alexa-Fluor 594, Molecular Probes) were diluted 1:1000 in blocking buffer and incubated with tissue sections for two hours at RT. .. Total cell population was also labeled in all tissue samples using 4′,6-diamidino-2-phenylindole (DAPI, Life Technologies).

Article Title: Implications of Chronic Daily Anti-Oxidant Administration on the Inflammatory Response to Intracortical Microelectrodes
Article Snippet: .. Following blocking, the primary antibody, diluted in blocking buffer, was added directly to tissue sections and allowed to incubate for 18 hours at 4° C. Unbound primary antibodies were removed using six subsequent washes in 1X PBS-T. Species-specific secondary antibodies (anti-rabbit or mouse Alexa-Fluor 488 and anti-rabbit or mouse Alexa-Fluor 594, Life Technologies) were diluted 1:1000 in blocking buffer, applied directly to tissue sections and incubated at room temperature for 2 hours. .. In addition to secondary antibodies, 4’6-diamidino-2-phenylindole (DAPI; Life Technologies) was diluted at 1:32,000 to co-label total cell nuclei.

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    Pbt2 Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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