Structured Review

Roche 1x gc rich solution
1x Gc Rich Solution, supplied by Roche, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/1x gc rich solution/product/Roche
Average 90 stars, based on 3 article reviews
Price from $9.99 to $1999.99
1x gc rich solution - by Bioz Stars, 2020-07
90/100 stars

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Related Articles

Polymerase Chain Reaction:

Article Title: Creating cancer translocations in human cells using Cas9 DSBs and nCas9 paired nicks
Article Snippet: .. 2X Master Mix 2: 1X GC-RICH solution (Roche FastStart Taq), 2X PCR Buffer with 20 mM MgCl2 (Roche FastStart Taq), 400 μM dNTP mix (Roche FastStart Taq), 4% DMSO, 0.01% Tween20, 0.01% NP40, 0.25 μl of SYBR® Green I (10,000x in DMSO, Sigma Aldrich) diluted in 2 mL 20% DMSO and Rox (concentrations depend on the real time PCR machine; 30 nM of Rox is used with MX3005P from Agilent). .. Primers for nested PCR FastStart Taq DNA polymerase (Roche) Real-time quantitative PCR machine (MX3005P, Agilent)

Article Title: Creating cancer translocations in human cells using Cas9 DSBs and nCas9 paired nicks
Article Snippet: .. 10X Lysis buffer: 100 mM Tris-HCL pH 8, 4.5% NP40, 4.5% Tween20 Proteinase K (New England Biolabs) 2X Master Mix 1: 1X GC-RICH solution (Roche FastStart Taq), 2X PCR Buffer with 20 mM MgCl2 (Roche FastStart Taq), 400 μM dNTP mix (Roche FastStart Taq), 4% DMSO, 0.01% Tween20, 0.01% NP40. .. 2X Master Mix 2: 1X GC-RICH solution (Roche FastStart Taq), 2X PCR Buffer with 20 mM MgCl2 (Roche FastStart Taq), 400 μM dNTP mix (Roche FastStart Taq), 4% DMSO, 0.01% Tween20, 0.01% NP40, 0.25 μl of SYBR® Green I (10,000x in DMSO, Sigma Aldrich) diluted in 2 mL 20% DMSO and Rox (concentrations depend on the real time PCR machine; 30 nM of Rox is used with MX3005P from Agilent).

Article Title: Preimplantation genetic diagnosis of hemophilia A
Article Snippet: .. The first PCR was carried out in a 40 μL reaction, consisting of 1X PCR buffer, 1.25 mmol/L MgCl2 , 0.35 mmol/L dNTP, each 0.5 μmol/L of OF and OR primer, 1X GC-RICH solution, and 1U Faststart Taq DNA polymerase (Roche Diagnostics GmbH, Mannheim, Germany). .. The PCR products were directly subjected to the second round of PCR by adding 10 μL PCR supplement with a similar PCR mixture to that used in the first PCR, except for the primer set (IF and IR).

SYBR Green Assay:

Article Title: Creating cancer translocations in human cells using Cas9 DSBs and nCas9 paired nicks
Article Snippet: .. 2X Master Mix 2: 1X GC-RICH solution (Roche FastStart Taq), 2X PCR Buffer with 20 mM MgCl2 (Roche FastStart Taq), 400 μM dNTP mix (Roche FastStart Taq), 4% DMSO, 0.01% Tween20, 0.01% NP40, 0.25 μl of SYBR® Green I (10,000x in DMSO, Sigma Aldrich) diluted in 2 mL 20% DMSO and Rox (concentrations depend on the real time PCR machine; 30 nM of Rox is used with MX3005P from Agilent). .. Primers for nested PCR FastStart Taq DNA polymerase (Roche) Real-time quantitative PCR machine (MX3005P, Agilent)

Real-time Polymerase Chain Reaction:

Article Title: Creating cancer translocations in human cells using Cas9 DSBs and nCas9 paired nicks
Article Snippet: .. 2X Master Mix 2: 1X GC-RICH solution (Roche FastStart Taq), 2X PCR Buffer with 20 mM MgCl2 (Roche FastStart Taq), 400 μM dNTP mix (Roche FastStart Taq), 4% DMSO, 0.01% Tween20, 0.01% NP40, 0.25 μl of SYBR® Green I (10,000x in DMSO, Sigma Aldrich) diluted in 2 mL 20% DMSO and Rox (concentrations depend on the real time PCR machine; 30 nM of Rox is used with MX3005P from Agilent). .. Primers for nested PCR FastStart Taq DNA polymerase (Roche) Real-time quantitative PCR machine (MX3005P, Agilent)

Lysis:

Article Title: Creating cancer translocations in human cells using Cas9 DSBs and nCas9 paired nicks
Article Snippet: .. 10X Lysis buffer: 100 mM Tris-HCL pH 8, 4.5% NP40, 4.5% Tween20 Proteinase K (New England Biolabs) 2X Master Mix 1: 1X GC-RICH solution (Roche FastStart Taq), 2X PCR Buffer with 20 mM MgCl2 (Roche FastStart Taq), 400 μM dNTP mix (Roche FastStart Taq), 4% DMSO, 0.01% Tween20, 0.01% NP40. .. 2X Master Mix 2: 1X GC-RICH solution (Roche FastStart Taq), 2X PCR Buffer with 20 mM MgCl2 (Roche FastStart Taq), 400 μM dNTP mix (Roche FastStart Taq), 4% DMSO, 0.01% Tween20, 0.01% NP40, 0.25 μl of SYBR® Green I (10,000x in DMSO, Sigma Aldrich) diluted in 2 mL 20% DMSO and Rox (concentrations depend on the real time PCR machine; 30 nM of Rox is used with MX3005P from Agilent).

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  • 90
    Roche 1x gc rich solution
    1x Gc Rich Solution, supplied by Roche, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/1x gc rich solution/product/Roche
    Average 90 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    1x gc rich solution - by Bioz Stars, 2020-07
    90/100 stars
      Buy from Supplier

    89
    Roche gc rich solution
    Gc Rich Solution, supplied by Roche, used in various techniques. Bioz Stars score: 89/100, based on 62 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gc rich solution/product/Roche
    Average 89 stars, based on 62 article reviews
    Price from $9.99 to $1999.99
    gc rich solution - by Bioz Stars, 2020-07
    89/100 stars
      Buy from Supplier

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