Structured Review

Thermo Fisher 1x dtt
1x Dtt, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/1x dtt/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
Price from $9.99 to $1999.99
1x dtt - by Bioz Stars, 2020-03
90/100 stars

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1x lds loading buffer
ief

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Related Articles

Western Blot:

Article Title: Retinoid receptor turnover mediated by sumoylation, ubiquitination and the valosin-containing protein is disrupted in glioblastoma
Article Snippet: .. Following IEF, the strip was equilibrated in 1X LDS loading buffer with 1X DTT (Invitrogen) and loaded into the single well of the ZOOM NuPAGE gel and the standard Western protocol was followed. .. RNA interference For siRNA transfections, cells were seeded into six well dishes at a density of 200,000 cells/well.

Electrofocusing:

Article Title: Retinoid receptor turnover mediated by sumoylation, ubiquitination and the valosin-containing protein is disrupted in glioblastoma
Article Snippet: .. Following IEF, the strip was equilibrated in 1X LDS loading buffer with 1X DTT (Invitrogen) and loaded into the single well of the ZOOM NuPAGE gel and the standard Western protocol was followed. .. RNA interference For siRNA transfections, cells were seeded into six well dishes at a density of 200,000 cells/well.

Stripping Membranes:

Article Title: Retinoid receptor turnover mediated by sumoylation, ubiquitination and the valosin-containing protein is disrupted in glioblastoma
Article Snippet: .. Following IEF, the strip was equilibrated in 1X LDS loading buffer with 1X DTT (Invitrogen) and loaded into the single well of the ZOOM NuPAGE gel and the standard Western protocol was followed. .. RNA interference For siRNA transfections, cells were seeded into six well dishes at a density of 200,000 cells/well.

Molecular Weight:

Article Title: Retinoid receptor turnover mediated by sumoylation, ubiquitination and the valosin-containing protein is disrupted in glioblastoma
Article Snippet: Proteins were separated by isoelectric focusing using the Ettan IPGphor II isoelectric focusing system (GE) and separated by molecular weight using 4–12% Bis-Tris ZOOM NuPAGE gel (Invitrogen). .. Following IEF, the strip was equilibrated in 1X LDS loading buffer with 1X DTT (Invitrogen) and loaded into the single well of the ZOOM NuPAGE gel and the standard Western protocol was followed.

Lysis:

Article Title: Retinoid receptor turnover mediated by sumoylation, ubiquitination and the valosin-containing protein is disrupted in glioblastoma
Article Snippet: 2D Western blot Cells were lysed in IEF lysis buffer (8 M urea, 1% NP-40, 10 mM DTT) with fresh protease and phosphatase inhibitors (Sigma-Aldrich). .. Following IEF, the strip was equilibrated in 1X LDS loading buffer with 1X DTT (Invitrogen) and loaded into the single well of the ZOOM NuPAGE gel and the standard Western protocol was followed.

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